Animal Reproduction最新文献

{"title":"Animal Reproduction journal and International Symposium on Animal Biology of Reproduction: Historical and personal reflections on two decades of exciting amalgamation of young and old science and scientists.","authors":"Luiz Renato de França","doi":"10.1590/1984-3143-AR2024-0078","DOIUrl":"https://doi.org/10.1590/1984-3143-AR2024-0078","url":null,"abstract":"<p><p>In 2024, the Brazilian College of Animal Reproduction (CBRA in Portuguese) is proudly celebrating its golden 50^th anniversary. Founded in 1974, CBRA has had a very productive and challenging journey of five decades, achieving many important milestones that have established it as a major society and its journal as a major reference in the field of animal reproduction, both in Brazil and internationally. Coincidentally, the <i>Animal Reproduction</i> journal and the International Symposium on Animal Biology (ISABR), both created and sponsored by CBRA, are also celebrating their 20^th and 10^th anniversary and edition, respectively, this year. These remarkable events are being celebrated in the city of Fortaleza, Brazil, during the 10^th edition of ISABR. As someone who had the privilege of playing a leading role in the creation and establishment of both <i>Animal Reproduction</i> journal and ISABR, I am honored to describe here the favorable circumstances that led to these significant achievements. The crucial steps and combined efforts required to make these institutions successful were unconditionally supported by the CBRA. Additionally, significant global networking and scientific collaborations, both individual and collective, have been pivotal in advancing the science and connecting the scientific community, spanning both young and experienced members, for decades. Finally, I hope that this historical article will inspire future generations of scientists in the field to continue CBRA's journey and leadership, ensuring the growth of <i>Animal Reproduction</i> and ISABR advancement to even higher standards.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240078"},"PeriodicalIF":1.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of temperature on gonadal differentiation and growth of <i>Leporinus friderici</i>.","authors":"Thiago Gonçalves de Souza, Mariana Roza de Abreu, Rafael Yutaka Kuradomi, Sergio Ricardo Batlouni","doi":"10.1590/1984-3143-AR2023-0158","DOIUrl":"10.1590/1984-3143-AR2023-0158","url":null,"abstract":"<p><p>This study aimed to investigate the effect of temperature on gonadal differentiation, growth, survival, and sex ratio of <i>Leporinus friderici</i> reared at 25 °C or 29 °C from 50 to 240 days after eclosion (DAE) in a water recirculation system. A total of 110 fish at 50 DAE (6.7 ± 0.1 cm and 6.1 ± 0.3 g) were equally and randomly distributed in 10 boxes (90 L) (11 fish/box, 5 boxes/temperature). One fish from each experimental unit was randomly sampled at 50, 70, 90, 110, 130, 150, 170, 190, 210 and 240 DAE. Female gonadal differentiation started at 150 DAE (11.4 ± 0.0 cm and 16.4 ± 0.0 g) at 25 °C and at 170 DAE (10.7 ± 0.7 cm and 27.7 ± 8.5 g) at 29 ºC, while testes differentiation only occurred at 29 °C from 190 DAE (12.1 ± 0.0 cm and 38.0 ± 0.0 g). Of 50 fishes sampled in each condition, 17 (12 females and five males) and three (three females) displayed gonadal differentiation at 29 °C and 25 °C, respectively. Final biometric values at 29 °C were twice those obtained at 25 °C, reaching 13.9 ± 0.65 cm and 57.3 ± 10.12 g <i>versus</i> 11.2 ± 0.39 cm and 28.5 ± 2.95 g, respectively. While temperature clearly influenced gonadal differentiation and growth, it had inconclusive effects on sex ratio. The higher temperature (29 °C) has direct implications for the production of this species, as it accelerates growth without causing mortality.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230158"},"PeriodicalIF":1.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of sub-clinical endometritis on early pregnancy predictors and proinflammatory cytokines in circulating immune cells in dairy cows.","authors":"Diego Angelo Schmidt Poit, Priscila Assis Ferraz, Gabriela de Andrade Bruni, Giulia de Andrade Bruni, Thiago Kan Nishimura, Igor Garcia Motta, Isabella Rio Feltrin, Guilherme Pugliesi","doi":"10.1590/1984-3143-AR2023-0144","DOIUrl":"10.1590/1984-3143-AR2023-0144","url":null,"abstract":"<p><p>In Experiment 1, PBMC were isolated from cows considered healthy or with SCE (n=6/group) on Days 0 (estrus) and 7 (diestrus) of a synchronized estrous cycle. In Experiment 2, on D21 (D0 was defined as the day of Fixed Timed Artificial Insemination (FTAI), cows were evaluated by ultrasonography to assess luteal blood perfusion and PBMC were isolated. On D32, cows were classified into: healthy pregnant (n=7), pregnant with SCE (n=4), healthy non-pregnant (n=8), and non-pregnant with SCE (n=10). Gene expression of ISGs (ISG15, OAS1, MX1 and IFI6) and proinflammatory cytokines (IL1-β, TNF-α and IFN-γ) were determined. Expression of ISG15, MX1, IFI6, TNF-α and IFN-γ did not differ between SCE and healthy cows and between Days 0 and 7. Expression of OAS1 and IL1-β were higher (P=0.02) on Day 7 than Day 0, regardlees of the SCE presence. In Exp.2, ISG15 abundance was 2.5-fold greater (P=0.0008), TNF-α was 2.2-fold greater (P=0.05), and IL1-β tended (P=0.06) to be 2.4-fold higher in pregnant than non-pregnant cows. Luteal blood perfusion was greater (P=0.01) in pregnant animals. In conclusion, OAS1 and IL1-β are transcripts upregulated in PBMC at diestrus, regardless of SCE occurrence. Proinflammatory cytokines are not affected by SCE occurrence, but IL1-β and TNF-α are upregulated in pregnant animals on D21 of pregnancy. ISG15 abundance is a good pregnancy predictor, regardless SCE presence.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230144"},"PeriodicalIF":1.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NPPC and AREG supplementation in IVM systems alter mRNA translation and decay programs-related gene expression in bovine COC.","authors":"Helena Fabiana Reis de Almeida Saraiva, Juliano Rodrigues Sangalli, Luana Alves, Juliano Coelho da Silveira, Flávio Vieira Meirelles, Felipe Perecin","doi":"10.1590/1984-3143-AR2023-0101","DOIUrl":"10.1590/1984-3143-AR2023-0101","url":null,"abstract":"<p><p>During oocyte meiosis resumption, a coordinated program of transcript translation and decay machinery promotes a remodeling of mRNA stores, which determines the success of the acquisition of competence and early embryo development. We investigated levels of two genes related to mRNA translation (<i>CPEB1</i> and <i>CPEB4</i>) and two related to mRNA degradation (<i>CNOT7</i> and <i>ZFP36L2</i>) machinery and found <i>ZFP36L2</i> downregulated in <i>in vitro</i>-matured bovine oocytes compared to <i>in vivo</i> counterparts. Thereafter, we tested the effects of a pre-IVM step with NPPC and a modified IVM with AREG on the modulation of members of mRNA translation and degradation pathways in cumulus cells and oocytes. Our data showed a massive upregulation of genes associated with translational and decay processes in cumulus cells, promoted by NPPC and AREG supplementation, up to 9h of IVM. The oocytes were less affected by NPPC and AREG, and even though ZFP36L2 transcript and protein levels were downregulated at 9 and 19h of IVM, only one (<i>KDM4C</i>) from the ten target genes evaluated was differently expressed in these treatments. These data suggest that cumulus cells are more prone to respond to NPPC and AREG supplementation <i>in vitro</i>, regarding translational and mRNA decay programs. Given the important nursing role of these cells, further studies could contribute to a better understanding of the impact of these modulators in maternal mRNA modulation and improve IVM outcomes.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230101"},"PeriodicalIF":1.6,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular damage without clinical manifestations in BALB/c mice experimentally infected with Zika virus.","authors":"Derick Mendes Bandeira, Arthur da Costa Rasinhas, Raphael Leonardo, Marcos Alexandre Nunes da Silva, Eduarda Lima Araujo, Gisela Freitas Trindade, Renata Tourinho Santos, Ygara da Silva Mendes, Ortrud Monika Barth, Debora Ferreira Barreto-Vieira","doi":"10.1590/1984-3143-AR2023-0124","DOIUrl":"10.1590/1984-3143-AR2023-0124","url":null,"abstract":"<p><p>In 2015-2016, the Zika virus (ZIKV) caused a major epidemic in the Americas, increasing cases of microcephaly and Guillain-Barré syndrome. During this period, the discovery of ZIKV sexual transmission intensified studies on the impact of this virus on the reproductive organs. For this study, 2-month-old male BALB/c mice were infected with 1.26 x 10⁶ PFU/mL of ZIKV in solution via the intravenous route. After three, seven, and fourteen days post-infection (DPI), blood and testicle samples were obtained to detect ZIKV RNA. The authors observed that the infected animals had slower weight gain than the control group. Viremia occurred only at 3DPI, and the ZIKV RNA was detected in one testis sample at 7DPI. The histopathological analysis of this organ revealed intense disorganization of the seminiferous tubules' structure, inflammatory infiltrate, necrosis, hemorrhage, fluid accumulation, congestion of blood vessels, and reduced sperm count. Ultrastructural analysis showed nuclear changes in tubule cells, activation of interstitial cells, and morphological changes in spermatozoa, in addition to fragmentation and decreased electron density of the genetic material of these cells. Thus, despite causing predominantly asymptomatic infections, ZIKV can cause significant subclinical and transient damage, including to male reproductive organs.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230124"},"PeriodicalIF":1.6,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is FSH combined with equine chorionic gonadotropin able to modify lipid metabolism in bovine superstimulated antral follicles?","authors":"Priscila Helena Santos, Fernanda Fagali Franchi, Sarah Gomes Nunes, Patricia Kubo Fontes, Alan Brunholi Giroto, Fernanda Mani, Anthony César de Souza Castilho","doi":"10.1590/1984-3143-AR2023-0063","DOIUrl":"10.1590/1984-3143-AR2023-0063","url":null,"abstract":"<p><p>Lipid metabolism is essential for ensuring oocyte maturation and embryo development. β-Oxidized fatty acids (FA) are a potent source of energy for cells, particularly for bovine somatic follicular cells. Superstimulatory protocols using follicle stimulating hormone (FSH) or FSH combined with equine chorionic gonadotropin (eCG) are capable of stimulating the follicular microenvironment and drive the expression of biomarker genes associated with lipid metabolism in the cumulus-oocyte complex (COC) for better embryo development. In this study, we assesed the effects of FSH and FSH/eCG protocols on the expression of genes related to lipid metabolism in bovine granulosa cells (GCs). Further, we measured triglyceride levels in follicular fluid (FF) obtained from both superstimulatd and non-superstimulated cows (synchronized cows). In summary, superstimulation with gonadotropins maintained the TG levels in bovine FF and ensured GCs mRNA abundance of <i>ACSL1, ACSL3, ACSL6, SCD, ELOVL5, ELOVL6, FASN, FADS2,</i> and <i>SREBP1.</i> We, however, found the abundance of <i>CPTIB</i> mRNA to be lower in GCs obtained from cows subjected to FSH/eCG protocols than synchronized cows. In conclusion, the findings of this study showed that ovarian superstimulation around the preovulatory phase has a mild impact on the lipid metabolism in GCs.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230063"},"PeriodicalIF":1.6,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory and anti-oxidant action of tadalafil in testicular regeneration process after heat stress.","authors":"Fernando Leonel da Silva, Fernanda Carolina Ribeiro Dias, Sandra Maria Torres, Virginia Maria Barros de Lorena, Sebastião Rogerio de Freitas Silva, Vinicius Vasconcelos Gomes de Oliveira, Emanoel Felipe de Oliveira, Pierre Castro Soares, Valdemiro Amaro da Silva","doi":"10.1590/1984-3143-AR2023-0095","DOIUrl":"10.1590/1984-3143-AR2023-0095","url":null,"abstract":"<p><p>Tadalafil, a potent phosphodiesterase inhibitor 5 (PDE-5), is commonly used for the management of erectile dysfunction. However, its therapeutic potential extends beyond this indication. This study aimed to investigate the impact of tadalafil on the recovery of testicular parenchyma in male Wistar rats exposed to testicular thermal stress. Fifty-four Wistar rats were subjected to testicular thermal stress and randomly assigned to receive either tadalafil treatment (TAD) or no treatment (control). TAD was administered intraperitoneally at a dose of either 0.9 mg/kg or 1.8 mg/kg. Biometric parameters, histopathological assessment of the testis, serum testosterone levels, oxidative stress, and interleukin levels were evaluated on days 7, 15, and 30 after thermal shock. The animals were euthanized at the end of each experimental period, and samples were collected. TAD treatment maintained testicular weight and reduced the testicular degenerative process up to day 7 post-injury. However, despite TAD therapy, serum testosterone levels were decreased in the treated groups at days 7 and 15 post-thermal stress. TAD also decreased TNF-α and NO levels at different doses but had no effect on IL-6. The treatment with TAD after heat shock demonstrated anti-inflammatory and antioxidant properties but did not prevent the aggravation of testicular lesions in subsequent periods, even with the systematic reduction in TNF-α and NO levels. Therefore, this selective PDE-5 inhibitor, at the dosages used, did not have a positive impact on testosterone levels during the post-thermal stress period, which could compromise the resumption of the spermatogenic process.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230095"},"PeriodicalIF":1.6,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA sequencing and gene co-expression network of <i>in vitro</i> matured oocytes and blastocysts of buffalo.","authors":"Priscila Di Paula Bessa Santana, Kenny da Costa Pinheiro, Lino César de Souza Pereira, Soraya Silva Andrade, Flávia Figueira Aburjaile, Priscilla do Carmo de Azevedo Ramos, Eduardo Baia de Souza, Nathalia Nogueira da Costa, Marcela da Silva Cordeiro, Simone do Socorro Damasceno Santos, Moysés Dos Santos Miranda, Rommel Thiago Jucá Ramos, Artur Luiz da Costa da Silva","doi":"10.1590/1984-3143-AR2023-0131","DOIUrl":"10.1590/1984-3143-AR2023-0131","url":null,"abstract":"<p><p>In reproductive technologies, uncovering the molecular aspects of oocyte and embryo competence under different conditions is crucial for refining protocols and enhancing efficiency. RNA-seq generates high-throughput data and provides transcriptomes that can undergo additional computational analyses. This study presented the transcriptomic profiles of <i>in vitro</i> matured oocytes and blastocysts produced <i>in vitro</i> from buffalo crossbred (<i>Bubalus bubalis</i>), coupled with gene co-expression and module preservation analysis. Cumulus Oophorus Complexes, obtained from slaughterhouse-derived ovaries, were subjected to <i>in vitro</i> maturation to yield metaphase II oocytes (616) or followed <i>in vitro</i> fertilization and culture to yield blastocysts for sequencing (526). Oocyte maturation (72%, ±3.34 sd) and embryo development (21.3%, ±4.18 sd) rates were obtained from three <i>in vitro</i> embryo production routines following standard protocols. Sequencing of 410 metaphase II oocytes and 70 hatched blastocysts (grade 1 and 2) identified a total of 13,976 genes, with 62% being ubiquitously expressed (8,649). Among them, the differentially expressed genes (4,153) and the strongly variable genes with the higher expression (fold-change above 11) were highlighted in oocytes (<i>BMP15</i>, <i>UCHL1</i>, <i>WEE1</i>, <i>NLRPs, KPNA7</i>, <i>ZP2,</i> and <i>ZP4</i>) and blastocysts (<i>APOA1</i>, <i>KRT18</i>, <i>ANXA2</i>, <i>S100A14</i>, <i>SLC34A2</i>, <i>PRSS8</i> and <i>ANXA2</i>) as representative indicators of molecular quality. Additionally, genes exclusively found in oocytes (224) and blastocysts (2,200) with specific biological functions were identified. Gene co-expression network and module preservation analysis revealed strong preservation of functional modules related to exosome components, steroid metabolism, cell proliferation, and morphogenesis. However, cell cycle and amino acid transport modules exhibited weak preservation, which may reflect differences in embryo development kinetics and the activation of cell signaling pathways between buffalo and bovine. This comprehensive transcriptomic profile serves as a valuable resource for assessing the molecular quality of buffalo oocytes and embryos in future <i>in vitro</i> embryo production assays.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230131"},"PeriodicalIF":1.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic review of hormonal strategies to improve fertility in rams.","authors":"Estela Garza-Brenner, Fernando Sánchez-Dávila, Keyla Mauleón-Tolentino, Cecilia Carmela Zapata-Campos, Carlos Luna-Palomera, Javier Hernandez-Melendez, Marisol Gonzalez-Delgado, José Fernando Vázquez-Armijo","doi":"10.1590/1984-3143-AR2024-0007","DOIUrl":"10.1590/1984-3143-AR2024-0007","url":null,"abstract":"<p><p>Reviewing the current state of knowledge on reproductive performance and productive traits in rams has many advantages. First, the compilation of this information will serve as a literature resource for scientists conducting research around the world and will contribute to the understanding of the data collected and interpreted by researchers on the different hormonal strategies used to improve reproductive performance in rams. Second, it will allow scientists to identify current knowledge gaps and set future research priorities in ram reproduction. Rams play an important role in the global flock economy, but their reproductive analysis has been limited in the use of hormonal technologies to increase the productivity of sheep flocks. In this review, we cite the most important works on six hormones that, in one way or another, modify the hypothalamus-pituitary-gonadal axis, at different doses, in and out of the reproductive season, breeds, application methods, among other factors. The overall aim is to increase the reproductive efficiency of rams in different scenarios and, in some cases, of other species due to the lack of limited information on rams.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20240007"},"PeriodicalIF":1.6,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance, reproductive status, and chromosomal instability in triploid Nile tilapias.","authors":"Williane Ferreira Menezes, Érika Ramos Alvarenga, Rafael Henrique Nóbrega, Luiz Renato França, Marcelo Rezende Luz, Ludson Guimarães Manduca, Franklin Fernando Batista da Costa, Vinícius Monteiro Bezerra, Arthur Francisco de Araújo Fernandes, Eduardo Maldonado Turra","doi":"10.1590/1984-3143-AR2023-0147","DOIUrl":"10.1590/1984-3143-AR2023-0147","url":null,"abstract":"<p><p>Reproductive control is one of the biggest challenges in tilapia production and triploidy was developed as an alternative to sterilization. In general, polyploids present chromosomal instability but for triploid Nile tilapia it has yet to be reported. This study evaluated the chromosomal instability from juveniles to adulthood, growth performance and gonadal status of tilapia hatched from eggs submitted or not to heat shock for triploid induction. Nile tilapia oocytes were fertilized (1,476 oocytes), half of the eggs were subjected to a four-minute shock in 41 °C water four minutes after fertilization and the other half were not (Control group). The eggs were incubated (at 27°C) and 160 larvae from the treated group hatched and survived after yolk sac absorption. The determination of ploidy was performed by flow cytometry at 85^th (juveniles) and 301^st (adults) days of age post yolk sac absorption. At the time of the first cytometry analysis there were 73 surviving juveniles from the treated group, and only 14 were confirmed triploid. However, at the analysis of adult ploidy, one out of 8 surviving adult tilapias from the 14 confirmed triploid juveniles remained triploid. Gonadal histology showed that the non-remaining triploids continued to produce gametes. The growth performance of triploid tilapia was initially superior to that of diploid tilapia during the juvenile phase, but similar in adults. Once the chromosome sets are lost and the tilapias become diploid again, at least in tissues with a high proliferation rate, such as the hematopoietic tissue that was analyzed (and possibly in gonads), all possible advantages of triploids are probably lost. Thus, our results suggest that, due to genomic instabilities, the triploid generation of tilapia has low efficiency.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230147"},"PeriodicalIF":1.7,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11129864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}