Jinzhu Meng, Jianhao Feng, Lilin Xiao, Nan Hu, Xianyong Lan, Shuilian Wang
{"title":"口服抑制素 DNA 疫苗促进大鼠精子发生。","authors":"Jinzhu Meng, Jianhao Feng, Lilin Xiao, Nan Hu, Xianyong Lan, Shuilian Wang","doi":"10.1590/1984-3143-AR2023-0079","DOIUrl":null,"url":null,"abstract":"<p><p>The objective of the present study was to evaluate the effects of a novel Inhibin (INH) DNA vaccine (C500/pVAX-asd-IS) on the immune response, reproductive hormone levels, and spermatogenesis of rats. Forty healthy male rats were divided into four groups, and respectively immunized (thrice, 14 d apart) with 1×10<sup>8</sup>, 1×10<sup>9</sup>, and 1×10<sup>10</sup> CFU of the recombinant inhibin vaccine (group C500/pVAX-asd-IS-L, C500/pVAX-asd-IS-M, and C500/pVAX-asd-IS-H) or 1×10<sup>10</sup> CFU C500. P/N values increased after vaccination and differed (<i>p</i> <0.05) at 7 d, and sharply increased at 14 d following the booster vaccination (<i>p</i> <0.01); The weight and volume of testes in C500/pVAX-asd-IS groups were increased (<i>p</i> < 0.05) at decapitation, respectively; Histological evaluation showed that the number of spermatogenic cells in the lumen was increased, and the cytoplasmic remnants of sperms were allergy increased significantly compared with the control group. Oral vaccination with INH DNA reduced (<i>P</i> < 0.05) serum concentrations of INH B, enhanced serum concentrations of testosterone (T) and FSH. Furthermore, mRNA expressions of <i>VIM</i> and <i>SMAD4</i> in the testes were increased in C500/pVAX-asd-IS-M and C500/pVAX-asd-IS-H groups (<i>p</i> < 0.05 or <i>p</i> < 0.01). The mRNA amount of <i>INHβ-B</i> in C500/pVAX-asd-IS-M group was greater than control group (<i>p</i> < 0.05).These results suggested that neutralization of endogenous INH through oral vaccination with INH DNA delivered by C500 strain successfully elicited a humoral immune response. INH gene immunization may have a positive effect on spermatogenesis and reproductive efficiency in male rats.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 4","pages":"e20230079"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452157/pdf/","citationCount":"0","resultStr":"{\"title\":\"Oral vaccination with inhibin DNA vaccine for promoting spermatogenesis in rats.\",\"authors\":\"Jinzhu Meng, Jianhao Feng, Lilin Xiao, Nan Hu, Xianyong Lan, Shuilian Wang\",\"doi\":\"10.1590/1984-3143-AR2023-0079\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The objective of the present study was to evaluate the effects of a novel Inhibin (INH) DNA vaccine (C500/pVAX-asd-IS) on the immune response, reproductive hormone levels, and spermatogenesis of rats. Forty healthy male rats were divided into four groups, and respectively immunized (thrice, 14 d apart) with 1×10<sup>8</sup>, 1×10<sup>9</sup>, and 1×10<sup>10</sup> CFU of the recombinant inhibin vaccine (group C500/pVAX-asd-IS-L, C500/pVAX-asd-IS-M, and C500/pVAX-asd-IS-H) or 1×10<sup>10</sup> CFU C500. P/N values increased after vaccination and differed (<i>p</i> <0.05) at 7 d, and sharply increased at 14 d following the booster vaccination (<i>p</i> <0.01); The weight and volume of testes in C500/pVAX-asd-IS groups were increased (<i>p</i> < 0.05) at decapitation, respectively; Histological evaluation showed that the number of spermatogenic cells in the lumen was increased, and the cytoplasmic remnants of sperms were allergy increased significantly compared with the control group. Oral vaccination with INH DNA reduced (<i>P</i> < 0.05) serum concentrations of INH B, enhanced serum concentrations of testosterone (T) and FSH. Furthermore, mRNA expressions of <i>VIM</i> and <i>SMAD4</i> in the testes were increased in C500/pVAX-asd-IS-M and C500/pVAX-asd-IS-H groups (<i>p</i> < 0.05 or <i>p</i> < 0.01). The mRNA amount of <i>INHβ-B</i> in C500/pVAX-asd-IS-M group was greater than control group (<i>p</i> < 0.05).These results suggested that neutralization of endogenous INH through oral vaccination with INH DNA delivered by C500 strain successfully elicited a humoral immune response. INH gene immunization may have a positive effect on spermatogenesis and reproductive efficiency in male rats.</p>\",\"PeriodicalId\":7889,\"journal\":{\"name\":\"Animal Reproduction\",\"volume\":\"21 4\",\"pages\":\"e20230079\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452157/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Reproduction\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1590/1984-3143-AR2023-0079\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1984-3143-AR2023-0079","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Oral vaccination with inhibin DNA vaccine for promoting spermatogenesis in rats.
The objective of the present study was to evaluate the effects of a novel Inhibin (INH) DNA vaccine (C500/pVAX-asd-IS) on the immune response, reproductive hormone levels, and spermatogenesis of rats. Forty healthy male rats were divided into four groups, and respectively immunized (thrice, 14 d apart) with 1×108, 1×109, and 1×1010 CFU of the recombinant inhibin vaccine (group C500/pVAX-asd-IS-L, C500/pVAX-asd-IS-M, and C500/pVAX-asd-IS-H) or 1×1010 CFU C500. P/N values increased after vaccination and differed (p <0.05) at 7 d, and sharply increased at 14 d following the booster vaccination (p <0.01); The weight and volume of testes in C500/pVAX-asd-IS groups were increased (p < 0.05) at decapitation, respectively; Histological evaluation showed that the number of spermatogenic cells in the lumen was increased, and the cytoplasmic remnants of sperms were allergy increased significantly compared with the control group. Oral vaccination with INH DNA reduced (P < 0.05) serum concentrations of INH B, enhanced serum concentrations of testosterone (T) and FSH. Furthermore, mRNA expressions of VIM and SMAD4 in the testes were increased in C500/pVAX-asd-IS-M and C500/pVAX-asd-IS-H groups (p < 0.05 or p < 0.01). The mRNA amount of INHβ-B in C500/pVAX-asd-IS-M group was greater than control group (p < 0.05).These results suggested that neutralization of endogenous INH through oral vaccination with INH DNA delivered by C500 strain successfully elicited a humoral immune response. INH gene immunization may have a positive effect on spermatogenesis and reproductive efficiency in male rats.
期刊介绍：
Animal Reproduction (AR) publishes original scientific papers and invited literature reviews, in the form of Basic Research, Biotechnology, Applied Research and Review Articles, with the goal of contributing to a better understanding of phenomena related to animal reproduction.
The scope of the journal applies to students, researchers and practitioners in the fields of veterinary, biology and animal science, also being of interest to practitioners of human medicine. Animal Reproduction Journal is the official organ of the Brazilian College of Animal Reproduction in Brazil.
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