Animal Reproduction最新文献

{"title":"Prevalence of testicular disorders in dogs and cats in Fortaleza from 2020 to 2023.","authors":"Amanda Bricio Pereira de Andrade, Raul Kelvin Silveira Maia, Maria Eduarda do Carmo Moura, Herlon Victor Rodrigues Silva, Lúcia Daniel Machado da Silva","doi":"10.1590/1984-3143-AR2024-0103","DOIUrl":"10.1590/1984-3143-AR2024-0103","url":null,"abstract":"<p><p>Considering the clinical importance and the frequent occurrence of several testicular pathologies, this study aimed to assess the prevalence of testicular disorders in dogs and cats and examine their association with species, breed, and age. In order to achieve this, ultrasound reports from the hospital's system were utilized. The odds ratio was calculated for species and breed, while Pearson's correlation test was used for age. A total of 1,282 dogs and 728 cats were examined. Of these, only the intact animals were considered susceptible to testicular conditions - 1,047 dogs (81.6%) and 218 cats (29.9%). Among dogs, 33.05% presented at least one testicular disorder, compared to 10.55% of cats. The most prevalent conditions were ectopia and degeneration, with neoplasia being frequently observed in canines. Other conditions identified included orchitis, fibrosis/microcalcification, cysts, hydrocele, monorchidism, torsion, and traumatic rupture. Canine species had a higher risk of testicular disorder and cryptorchidism. In dogs, age was strongly and positively related (p<0.001) to a higher prevalence of disorders. For general testicular disorders, poodle and pinscher breeds showed higher risks, while rottweilers had a lower risk compared to mixed-breed dogs. For ectopia, pinscher, shih-tzu, and poodle breeds had higher risks. Thus, it is evident that testicular , while less prevalent conditions deserve greater attention . In felines, the occurrence is less frequent and congenital conditions like ectopia and monorchidism are more common.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 2","pages":"e20240103"},"PeriodicalIF":2.3,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13004553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celiotomy to collect spermatogonial stem cells in <i>Brycon orbignyanus</i> for species preservation.","authors":"Laís Gonçalves da Silva, Karel Gelina Torres-Lozano, Rômulo Batista Rodrígues, Thaiza Rodrigues de Freitas, Jayme Aparecido Povh, Louise Nex Spica, Nathalia Dos Santos Teixeira, Douglas Cosme Selle, Jhony Lisbôa Benato, Raquel Santos Dos Santos, Eduardo Thomé Nicoleti, Renata Villar Dantas, Danilo Pedro Streit","doi":"10.1590/1984-3143-AR2025-0085","DOIUrl":"10.1590/1984-3143-AR2025-0085","url":null,"abstract":"<p><p>Surgical techniques in aquatic species are underdeveloped, despite these species comprising a significant superclass among vertebrates. In the context of species preservation, studies involving the use of reproductive tissues typically involve the euthanasia of donors. Thus, in animals at high risk of extinction, the practice of sacrificing them presents a point of contradiction between ex situ conservation efforts. The objective of this study was to compare the viability of spermatogonia stem cells (SSCs) collected from <i>Brycon orbignyanus</i> using conventional methods (euthanasia) versus a surgical procedure. Lateral celiotomy was performed on 27 immatures males to obtain a portion of gonadal tissue. The fish were divided into three groups (n=9), with each group receiving polyglactin 910, polyester, or catgut sutures, respectively, for celiorrhaphy. Dermorraphy was performed using nylon sutures in all groups. A. An additional euthanasia group consisted of nine animals exposed to 20 mg/L of propofol. The survival rate over a 48-hour period was 100% for the polyglactin 910 group and 77.7% for the polyester and catgut groups. Additionally, the viability of SSCs was similar between the euthanasia and surgical procedures. The lateral celiotomy technique is feasible for obtaining SSCs in fish with laterally compressed anatomy, such as <i>B. orbignyanus</i>. Additionally, the technique allows the preservation of SSCs as a model for endangered fish species.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250085"},"PeriodicalIF":2.3,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yolk sac development in dogs: from morphology to functional aspects.","authors":"Caio Garcia Barbosa da Silva, Julia Rodrigues Greghi, Maria Fernanda Frasson Pontes, Guilherme Schiess Cardoso, Maria Isabel Mello Martins, Carlos Eduardo Ambrósio, Phelipe Oliveira Favaron","doi":"10.1590/1984-3143-AR2025-0104","DOIUrl":"10.1590/1984-3143-AR2025-0104","url":null,"abstract":"<p><p>The yolk sac (YS) is a multifunctional and essential structure for early maternal-fetal interactions, playing roles in nutrient supply, organogenesis, immune modulation, and hematopoiesis. Unlike most species, dogs maintain the YS until the end of pregnancy, yet little is known about its functional activity, particularly in later stages. Animal models are crucial for exploring maternal-fetal processes and offering insights into comparative development, including in humans. This study aimed to analyze the YS structure in canine embryos and fetuses at early (up to 20 days, n = 6), middle (21-40 days, n = 6), and late (41-60 days, n = 6) stages. Gestational age was estimated using crown-rump length (CRL) and external anatomy. Samples from the university's Veterinary Hospital and neutering campaigns in Londrina (Paraná, Brazil) were fixed in 10% formaldehyde for morphological analysis (H&E, PAS, and Picrosirius red) or 4% paraformaldehyde for OCT4 immunostaining (CEUA/UEL protocol number 029.2022). Histologically, the YS showed a trilaminar structure with blood islands in the intermediate layer surrounded by mesenchyme. Within these islands, undifferentiated cellular niches (OCT4+) were identified. During gestation, the YS changed from a thin, smooth, pale-red membrane to a wrinkled structure with narrowed ends and a thickened central region, resembling a \"whale tail.\" The red color, indicative of vascularization, intensified with blood island enlargement. Collagen secretion, particularly type I, progressively increased around the blood islands. Glycoprotein deposits persisted until late gestation, as shown by PAS staining. These findings highlight the structural, secretory, and reserve roles of the canine YS throughout pregnancy.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250104"},"PeriodicalIF":2.3,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormone residues are not detected in zero water exchange biofloc system during tilapia masculinization even in higher feed input.","authors":"Dara Cristina Pires, Érika Ramos de Alvarenga, Franklin Fernando Batista da Costa, Kelly Moura Keller, José Fernandes Bezerra, Mariana Parrini Ferreira, Gabriela Lago Biscoto, Isabela Lopes Samary, Karen Beatriz Guerra Lima, Vinícius Monteiro Bezerra, Caroline Lopes de Melo, Lee Deyver Carvalho Pena Mansur, Williane Ferreira Menezes, José Fernando Paz Ramírez, Luiza Fujii Almeida, Daiana Dos Reis Pelegrine, Marcelo Rezende Luz, Eduardo Maldonado Turra","doi":"10.1590/1984-3143-AR2025-0106","DOIUrl":"10.1590/1984-3143-AR2025-0106","url":null,"abstract":"<p><p>How intensive the masculinization period of Nile tilapia can be in biofloc technology (BFT) based on higher stocking densities and a zero-water exchange protocol to mitigate the environmental impact has not been studied yet and needs investigation. Thus, our objective was to determine the optimal stocking density for reduction of hormonal effluent in BFT, better growth and masculinization rate of Nile tilapia larvae, and lower total variable cost. Five stocking densities (1.5, 3, 4.5, 6, and 7.5 larvae ∙ L^-1) with four replicates were tested. Tilapias were fed with 60 mg of 17α-methyltestosterone (MT) ∙ Kg^-1 of feed for 28 days, and with hormone-free feed until the common selling body weight of 1g. Water quality variables showed no significant differences between treatments, except for total settleable solids, pH, and final total organic carbon, that had a negative, positive and quadratic linear pattern with the increasing of stocking density, respectively. Growth performance variables such as final body weight, specific growth rate, survival and individual feed intake (and consequently hormone input) were superior at the lower stocking density. Masculinization rate (98.87%) did not differ among treatments, but total variable cost increased to produce 1g fingerlings in higher stocking densities. Hormone residue was not detected in BFT water of any treatment 12 hours after the last feeding so, in conclusion, even being the highest hormone input in the system, 1.5 larvae ∙ L^-1 is the best level of stocking density studied for tilapia masculinization in a zero-water exchange BFT.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250106"},"PeriodicalIF":2.3,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of techniques and conditions for <i>in vitro</i> ovulation induction in <i>Astyanax altiparanae</i>.","authors":"Cristiane Fernanda Benevente, Mariana Roza de Abreu, Queila Carla Ameno Alves, Laíza Maria de Jesus Silva, Roosevelt Passos Barbosa, Samyra Maria Dos Santos Nassif Lacerda, Sergio Ricardo Batlouni","doi":"10.1590/1984-3143-AR2025-0003","DOIUrl":"10.1590/1984-3143-AR2025-0003","url":null,"abstract":"<p><p>This study aimed to establish <i>in vitro</i> culture conditions for inducing ovulation in lambari (<i>Astyanax altiparanae</i>). All fish received a priming dose of either 0.6 mg/kg or 100 IU hCG/kg (human chorionic gonadotropin) <i>in vivo</i>. In experiment 1, 0.6 mg and 5.4 mg of carp pituitary extract (CPE)/ kg of ovarian fragment were tested as resolving doses. In experiment 2, prostaglandin F2α (PGF_2α) analogue at 100 ng/mL was added to 5.4 mg CPE/kg as the resolving dose. Since ovulation did not occur, in experiment 3 and 4, we compared diverse forms of obtaining follicles, comparing manual follicle dissociation and collagenase at 100, 200, and 400 CDU/mL. Additionally, in experiment 4, the resolving dose of CPE and hCG was replaced by 1 µg/mL 17α,20β-dihydroxy-4-pregnen-3-one (DHP). Ovulation was successful only in Experiment 4, using mechanically dissociated follicles with a priming dose of either CPE or hCG and 1 µg/mL DHP as the resolving dose. Key findings include that 5% CO₂ is unnecessary, mechanical dissociation of ovarian fragments is optimal, a priming dose of CPE or hCG is required, and DHP at 1 µg/mL is effective. These results establish a standardized protocol for <i>in vitro</i> ovulation induction in <i>A. altiparanae</i>, offering a valuable tool to study ovarian function and spawning failure in tropical species.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250003"},"PeriodicalIF":2.3,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin mitigates dichlorvos-induced derangements in the testes and epididymis of male Wistar rats.","authors":"Olumide Samuel Ajani, Ekundayo Stephen Samuel","doi":"10.1590/1984-3143-AR2024-0098","DOIUrl":"10.1590/1984-3143-AR2024-0098","url":null,"abstract":"<p><p>Dichlorvos (DDVP) is a widely used organophosphate insecticide. The reports of its toxicity and induction of oxidative stress have warranted the search for an antidote. Melatonin (MLT), a hormone found naturally in the body is known to mitigate oxidative stress. Therefore, the present study aimed to investigate the protective effects of melatonin on dichlorvos-induced toxicity in the testes and epididymis. Sixty (60) 10 weeks of male Wistar rats (160 ± 10g) randomly grouped into four (n=15, A-D) were used and treated as follows: A (Control): corn oil (1 mL/kg body weight), B: MLT (10 mg/kg), C: DDVP (1.6 mg/kg), and D: DDVP and MLT. Except for the MLT intra-peritoneal treatment, other test samples were administered orally for 45 days. Histomorphometry and histological examinations were conducted on the testes and epididymis 24h, 14 days, and 45 days after treatment. There was a significant decrease in the epithelial length of the epididymis of rats treated with DDVP at 24h and 45 days post-treatment compared to the control. MLT significantly increase the mean epididymal epithelial length at 24h and 45 days post-treatment in the co-treated group. The DDVP-induced significant reduction in the testes' luminal diameter was reversed by MLT in the co-treated group. MLT, DDVP (45th-day treatment), and DDVP+MLT (14th and 45th-day treatment) increased the epididymal luminal diameters significantly compared to the control. Meanwhile, DDVP altered both the testicular and epithelial architectures and caused atrophy of the seminiferous tubules and spermatogenic arrest with atrophy of epithelial tubules, especially following 14- and 45-day treatment. These lesions were reversed in the co-exposed rats by MLT. In conclusion, melatonin mitigates the derangements induced by dichlorvos in the testes and epididymis.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20240098"},"PeriodicalIF":2.3,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunolocalization of aromatase and estrogen and androgen receptors in the gonads and mesonephros of <i>Podocnemis expansa</i> during the first year of life.","authors":"Maria Fabiele Silva Oliveira, Layla Ianca Queiroz Rocha, Lucas Castanhola Dias, André de Macedo Medeiros, Moacir Franco de Oliveira, Carlos Eduardo Bezerra de Moura, Marcela Dos Santos Magalhães","doi":"10.1590/1984-3143-AR2025-0050","DOIUrl":"10.1590/1984-3143-AR2025-0050","url":null,"abstract":"<p><p>The endocrine regulation of testicular and ovarian development during early life in chelonians remains poorly understood, despite major morphophysiological changes occurring in this period. This study investigated the immunolocalization of estrogen (ER1 and ER2), androgen (AR), and aromatase (AROM) in the gonads and mesonephros of <i>Podocnemis expansa</i> during the first year after hatching (n = 5; males: 1, 3, 12 months; females: 2, 4 months). After euthanasia, gonads and mesonephros were collected, fixed in 10% buffered formaldehyde, and processed for immunohistochemistry. The intensity of immunoreactions varied according to age and sex, but not with tissue distribution. AROM, AR, ER1, and ER2 were detected in both gonads and mesonephros, confirming the persistence of steroidogenic and steroid-responsive activity after hatching. In males, AROM showed weak to moderate labeling in germ and interstitial cells of seminiferous tubules, whereas ER2 expression predominated in spermatogonia and interstitial endocrine cells. In females, AROM and AR were moderately expressed in the germinal epithelium and oogonia at two months, decreasing at four months, while ER2 persisted in follicles and oogonia. These findings suggest a dynamic endocrine environment influencing posthatch gonadal maturation, with the mesonephros acting as a transient extragonadal source of steroids. Despite the small, sex-unbalanced sample (due to lack of dimorphism), the results provide valuable baseline information and support future quantitative and functional studies on the reproductive endocrinology of <i>P. expansa</i> and other chelonians with temperature-dependent sex determination.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250050"},"PeriodicalIF":2.3,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the use of eCG on the pregnancy rate in dairy cows treated with a prolonged proestrus protocol in the Ecuadorian Amazon.","authors":"Manuel Edelberto Ortega-Coello, Orlando Roberto Quinteros-Pozo, Pablo Roberto Marini, Gabriel Amílcar Bó","doi":"10.1590/1984-3143-AR2025-0084","DOIUrl":"10.1590/1984-3143-AR2025-0084","url":null,"abstract":"<p><p>The objective of this study was to evaluate the effect of eCG on the time of ovulation, preovulatory follicle diameter and pregnancy rate in Holstein cows with a prolonged proestrus protocol in the Ecuadorian Amazon. This research was conducted between October 2018 to October 2019. We used 447 multiparous cows, milked once a day, with an average milk production of 8 to 9 liters cow/day. Two treatments were used, T1 with the addition of eCG (206 cows); and T2 without the application of eCG (241 cows). The pregnancy rate was higher in cows that received eCG (59.2%, T1) than in cows that did not receive eCG (45.6% T2: P<0.05), but the presence or absence of estrus did not differ (P>0.05) among groups (T1: 70.4%), T2: 65.6%). The diameter of the dominant follicle at P4 device removal (T1: 9.5 ± 0.6 mm vs T2: 9.7 ± 0.7 mm), as well as at the time of artificial insemination (T1: 11.6 ± 0.7mm vs T2: 11.2 ± 1.1mm) was not different among groups (P>0.05). However, the diameter of the dominant preovulatory follicle was greater (P<0.05) in cows in T1 (13.5 ± 1.1 mm) than those in T2 (12.5 ± 1.6 mm), with a follicular growth rate of 1.3 ± 0.1 mm/day and 0.9 ± 0.1 mm/day, for T1, T2 respectively (P<0.05). The mean ovulation time from device removal was shorter for T1 (94.7 h; P<0.05) than for T2 (101.5 h). The diameter of the CL was greater (P<0.05) for T1 (20.8 ± 2.3 mm) than T2 (19.1 ± 3.6 mm), while plasma concentrations of P4 did not differ among groups (P>0.05). In conclusion, the addition of eCG to the J-Synch protocol increased the diameter of the preovulatory follicle and the resulting CL, which favored the pregnancy rate in cows in the Ecuadorian Amazon.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250084"},"PeriodicalIF":2.3,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of calcium chloride and formalin injection into mediastinum testis on testosterone level, testicular tissue and semen quality in dogs.","authors":"Zehra Coşkun Bahçe, Fikret Karaca","doi":"10.1590/1984-3143-AR2025-0041","DOIUrl":"10.1590/1984-3143-AR2025-0041","url":null,"abstract":"<p><p>Intratesticular injection of chemical agents in dogs has been investigated for nearly half a century as promising methods for nonsurgical sterilization. This study was conducted to determine the effects of formalin and calcium chloride injection in mediastinum testis on semen quality, testicular width, testosterone level and histopathological changes in dogs, and to evaluate the usability and success of the intra mediastinum testis injection technique in chemical castration. Eighteen adult male dogs were divided into 3 groups including a control (0.5 ml 0.9% saline solution), formalin (5%) and CaCl₂ (50%) into the mediastinum. Testicular diameter, spermatological characteristics and testosterone concentrations were determined on days 0, 22 and 44 following applications. The dogs were castrated on 45 day and the testicles were sent to the laboratory for histopathological examinations. Formalin and CaCl₂ administered groups showed apparent enlargement and firmness of the testis with symptoms of discomfort and pain. One dog in the formalin group and two dogs in the CaCl₂ group developed scrotal inflammation within three days, which progressed to scrotal ulceration and fistula formation. On day 44 after treatment, it observed that sperm motility and sperm concentration in the formalin and CaCl₂ groups were decreased compare to control group while dead and abnormal sperm rates were increased (<i>P</i><0.05). The testosterone concentration significantly increased in CaCl₂ compare to control and formalin groups on day 44 (<i>P</i><0.05). In histopathological evaluations, moderate degenerative changes were detected in the testicular tissue of the formalin group. The findings of this study showed that CaCl₂ and formalin injections into the mediastinum testis under ultrasonography guidance are easily to perform and could be alternative to both the intratesticular injection and the surgical technique.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250041"},"PeriodicalIF":2.3,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphometric characterization of the uterus and placenta in Piau and Commercial sows during early gestation.","authors":"Tânia Fernandes Martins, Lívia Maria Dos Reis Barbosa, Luiz Otávio Guimarães Ervilha, Mariana Machado Neves, Alysson Saraiva, José Domingos Guimarães, Mateus Guimarães Dos Santos, Paula da Fonseca Pereira, Simone Eliza Facioni Guimarães","doi":"10.1590/1984-3143-AR2025-0070","DOIUrl":"10.1590/1984-3143-AR2025-0070","url":null,"abstract":"<p><p>Pregnancy induces critical physiological adaptations to support embryonic development and fetal survival. This study compared endometrial and placental phenotypic and histomorphometric characteristics of Piau and Commercial sows at two gestational ages (25 and 35 days). Twelve sows (six Piau and six Commercial) were evaluated in a randomized design, with samples collected from three regions of the right uterine horn of each animal. Histomorphometric analyses were performed using microscopy and ImageJ software. Statistical analyses employed linear mixed-effects models, with Shapiro-Wilk and Levene's tests applied to assess normality and homogeneity of variances, respectively. At 25 days of gestation, Commercial sows showed greater uterine and ovarian weights, a higher number of corpora lutea, and longer uterine horn horns, reflecting genetic selection for reproductive efficiency. Conversely, Piau sows exhibited more advanced embryonic development at this stage, with fetuses of greater size. At 35 days, the phenotypic superiority of Commercial sows persisted, while Piau fetuses maintained greater weight and length, indicating distinct temporal growth dynamics. Histomorphometric analyses at 25 days revealed that Commercial sows had increased placental connective tissue deposition and thicker endometrial epithelium, whereas Piau sows presented larger placental vascular area, as well as enhanced endometrial vascularization and glandular density across all uterine regions. At 35 days, no significant differences were observed in placental vascular area and endometrial vascularization; however, subtle trends in connective tissue development suggested ongoing placental differentiation. These findings highlight distinct reproductive strategies between Piau and Commercial sows, with potential implications for embryonic development and gestational success. Altogether, the results confirm that genetic background influences uterine and placental morphology during early gestation.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250070"},"PeriodicalIF":2.3,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}