Animal Reproduction最新文献

{"title":"High male proportions of Nile tilapia in a zero-water exchange biofloc system even at lower methyltestosterone concentration in the feed and water temperature below ideal.","authors":"José Fernando Paz Ramírez, Érika Ramos de Alvarenga, Franklin Fernando Batista da Costa, Kelly Moura Keller, Ana Paula Campos, Natan Paulo Bento Pio, Rafael Hiroaki Ito, Lee Deyver Carvalho Pena Mansur, William Gleidson Alves Torres, Vinícius Monteiro Bezerra, Dara Cristina Pires, Laryssa Evelyn Santos Soares, Guilherme Figueira Gonçalves, Gabriela Lago Biscoto, Marcelo Rezende Luz, Eduardo Maldonado Turra","doi":"10.1590/1984-3143-AR2025-0118","DOIUrl":"10.1590/1984-3143-AR2025-0118","url":null,"abstract":"<p><p>Since temperature plays an important role in feed consumption, chemical reactions and metabolism, it was hypothesized that it is below ideal it could interfere in the masculinization of tilapia in biofloc system (BFT) and the ideal methyltestosterone (MT) concentration in the feed. The masculinization rates of Nile tilapia in a zero-water exchange BFT (and without clarification) at 25°C and 28 °C combined with concentrations of MT in the feed (0 (control), 10, 20, 30 and 40 mg ∙ Kg^-1 of feed) were evaluated using 3 replicates per treatment (30 tanks of 50 liters, 2 larvae ∙ L^-1). Larvae were fed five times a day for 28 days. The water quality and growth performance did not diverge between MT concentrations (p > 0.05). Larvae grew 2.7 times higher in 28°C than 25°C. The control treatments did not differ from each other for male proportion (mean = 66,75%) but differed from all hormonal treatments. These treatments presented masculinization rates above 98.7% and 96.9%, at temperatures of 25°C and 28°C, respectively, and did not differ from each other at the same MT concentrations. Therefore, it is feasible to use an even lower concentration (10 mg of MT ⋅ Kg^-1 of feed) in a zero-water exchange BFT, regardless of these temperatures. At the lower temperature, the input of MT in the system was smaller due to smaller feed intake, however the fingerlings would take longer to reach commercial body weight. Furthermore, after 2 hours of the last hormone feeding, MT residues were not detected in any biofloc/water mixture samples.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250118"},"PeriodicalIF":2.3,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian structures modulate cellular aggregation and gene expression in oviductal isthmus cells.","authors":"Isabelle Cristina Pereira, Ana Carolina Bahia Teixeira, Raphael Rocha Wenceslau, Erika Cristina Jorge, Paola Maria da Silva Rosa, Julia Meireles Nogueira, Jade Carceroni de Sousa Carvalho, Gabriela Ponath Peruzzo, José de Oliveira Carvalho, Leticia Zoccolaro Oliveira","doi":"10.1590/1984-3143-AR2025-0075","DOIUrl":"10.1590/1984-3143-AR2025-0075","url":null,"abstract":"<p><p>The presence of follicles and the corpus luteum (CL) in the ovarian surface plays a key role in determining the morphological and molecular fate of the female reproductive tract. However, the specific response of the isthmus epithelium to these ovarian structures remains poorly understood. This study hypothesizes that distinct ovarian structures differentially affect both the cellular aggregate-forming capacity of oviductal isthmus cells and the expression of <i>ESR1, ESR2</i>, and <i>PGR</i> genes. Reproductive tracts were categorized into three groups: ovaries with ≤5 mm follicles (small follicles group; SF); ovaries with follicles between 8-10 mm (large follicles group; LF); and ovaries containing active corpus luteum (CL Group). Isthmus cells from the three groups were cultivated to form cellular aggregates (oviductal explants) during 24 h. Moreover, the expression levels of <i>ESR1, ESR2</i>, and <i>PGR</i> genes were analyzed in the isthmus cells of the experimental groups. The isthmus cells of LF group showed an increased number of cellular aggregates than SF and CL group. Additionally, the SF group presented more aggregates than the CL group. Gene expression analysis revealed that <i>ESR1</i> expression was higher in the SF group than in the LF group. Moreover, <i>PGR</i> expression was greater in the CL than in the SF group, as well as in the LF than in the SF group. In conclusion, ovarian structures impact the cellular aggregate formation capacity and the gene expression of ovarian steroid receptors in isthmus cells.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250075"},"PeriodicalIF":2.3,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147430431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of weaning age on pregnancy rates in Braford beef heifers bred at 13 months.","authors":"Maria Eduarda Cocco Dallanóra, José Fernando Piva Lobato, Javier Alexander Bethancourt-Garcia, Mariana Assis Borges, Rangel Fernandes Pacheco, João Restle, Ricardo Zambarda Vaz","doi":"10.1590/1984-3143-AR2025-0043","DOIUrl":"10.1590/1984-3143-AR2025-0043","url":null,"abstract":"<p><p>The aim of this study was to evaluate the probability of pregnancy in heifers weaned at different ages and bred at 13 to 15 months old. A total of 121 Braford heifers were used, weaned as calves at 77 days (early) or 147 days (conventional) of age. To develop the statistical models of reproductive performance, factors related to the development of the heifers were analyzed. The analysis included a diagnosis of multicollinearity using the Pearson correlation matrix, adjusting the model by means of the Hosmer and Lemeshow test. The response variable, rate of pregnancy, was analysed using the LOGISTIC procedure. Beginning with a weight of 271 kg and an age of 402 days at the start of the breeding season, the pregnancy rates increased by 18.4% and 29.0%, respectively for every 15 kg increase in body weight and 10-day increase in age. However, a reduction of 15 kg in body weight and of 10 days in age reduced the pregnancy rates in the heifers by 15.5% and 22.5%. An increase or reduction of 0.100 kg in the average daily gain between early weaning and conventional weaning represented an increase of 44.6% and a reduction of 30.9% in the chances of pregnancy. Early-weaned heifers require correct nutritional management to allow satisfactory postweaning weight gains so as not to compromise their reproductive performance.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250043"},"PeriodicalIF":2.1,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small extracellular vesicles from follicular fluid as transport carriers of <i>LHR</i>: possible mediators of follicle growth and dominance acquisition in bovine reproduction.","authors":"Luca Angi Souza, Nico G Menjivar, Ahmed Gad, Paulo Henrique Groppo Rodrigues, Letícia Rabello da Silva Sousa, Paola Maria da Silva Rosa, Alessandra Bridi, Dawit Tesfaye, Juliano Coelho da Silveira","doi":"10.1590/1984-3143-AR2025-0051","DOIUrl":"10.1590/1984-3143-AR2025-0051","url":null,"abstract":"<p><p>Luteinizing hormone (LH) plays a crucial role in follicle development, ovulation induction, and the regulation of key reproductive events. However, the efficacy of LH within the follicular microenvironment largely depends on the capacity of follicular cells to express its receptor. This study aims to investigate whether granulosa cells (GCs) can acquire <i>LHR</i> through extracellular vesicles (sEVs) present in follicular fluid (FF) from follicles of varying sizes. In the first experiment, GCs and sEVs were collected from the FF of small (3-5 mm), medium (5.1-7 mm), and large (7.1-9 mm) ovarian follicles from <i>Bos taurus indicus</i> cows. In the second experiment, GCs and sEVs were collected from the FF of small (3-6 mm) and large (8-14 mm) follicles from <i>Bos taurus taurus</i> cows. Initially, we assessed the ability of sEVs to carry <i>LHR</i> mRNA by comparing its expression profiles in sEVs derived from different size follicles. Our findings revealed that as follicular development progresses, <i>LHR</i> levels in FF sEVs decrease, while in corresponding GCs, from which the sEVs primarily originate, show increased <i>LHR</i> expression. To further investigate whether GCs represent an additional source of FF sEVs carrying LHR mRNA, GC cultures were established and sEVs secreted into the culture medium (ME-sEVs) were analyzed for <i>LHR</i> mRNA levels. A similar pattern was observed in ME-sEVs derived from GCs of small versus large follicles, with decreased LHR mRNA levels in sEVs secreted by GCs from large follicles compared to small follicles. This suggests that <i>LHR</i> is likely packaged into sEVs in small follicles stage, and shuttled into follicular cells during follicular growth, preparing them for the ovulatory stimulus. Our study uncovers a possible mechanism of <i>LHR</i> acquisition by GCs, which involves EVs and can possibly be involved in follicle quality and ability to respond to LH stimulus.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20250051"},"PeriodicalIF":2.1,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental competence of alpaca oocytes matured <i>in vivo</i> with seminal plasma and following additional <i>in vitro</i> maturation.","authors":"Nancy Milagros Silva Huanca, Víctor Hugo Cornelio Díaz, Luis Antonio Auqui Rojas, Alexis Ivan Huaman Apaza, Wilfredo Huanca López","doi":"10.1590/1984-3143-AR2024-0138","DOIUrl":"10.1590/1984-3143-AR2024-0138","url":null,"abstract":"<p><p>The aim of this study was to evaluate the effects of <i>in vivo</i> oocyte maturation using seminal plasma alone or in combination with an additional period of <i>in vitro</i> maturation (IVM) on the developmental competence of alpaca oocytes. The experiment was conducted in Lima, Peru, with twelve adult female alpacas. Follicular ablation of the dominant follicle was performed to initiate a new follicular wave. After 36 hours, a superstimulation protocol with 750 IU of eCG was administered intramuscularly (IM). Four days later, 2 mL of seminal plasma was administered IM to promote <i>in vivo</i> oocyte maturation. Cumulus-oocyte complexes (COCs) were retrieved via ovum pick-up 20 hours post-treatment, morphologically evaluated, and allocated into three groups: (T1) no additional IVM, (T2) 12 hours of additional IVM, and (T3) 18 hours of additional IVM. Oocyte developmental competence was assessed using a 26 µM brilliant cresyl blue (BCB) staining protocol for 90 minutes. COCs were classified as BCB positive (blue ooplasm) or BCB negative (unstained ooplasm) and subsequently denuded and fixed for nuclear maturation assessment via orcein staining. No significant differences (p=0.14) were observed in the percentage of expanded COCs across groups (71.4%, 81.8%, and 54.6% for T1, T2, and T3, respectively). The proportion of COCs reaching the metaphase II (MII) stage was higher (p<0.05) in T3 (54.6%), while the developmental competence rate was greatest (p<0.05) in T2 (100%). However, no differences (p=0.21) were detected in the proportion of BCB positive MII stage COCs across groups. In conclusion, alpaca oocytes matured <i>in vivo</i> with seminal plasma require 12 to 18 hours of IVM to achieve optimal nuclear maturation and developmental competence.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20240138"},"PeriodicalIF":2.1,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12822321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146027855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental models for subclinical hypocalcemia and endometritis induction in cattle: a literature review.","authors":"Jerbeson Hoffmann da Silva, André Gustavo Cabrera Dalto, Eduardo Schmitt, Bernardo Garziera Gasperin, Carlos Bondan, Monique Tomazele Rovani","doi":"10.1590/1984-3143-AR2024-0143","DOIUrl":"10.1590/1984-3143-AR2024-0143","url":null,"abstract":"<p><p>Serum calcium fluctuations are common during the peripartum period of dairy cattle and several studies have attempted to demonstrate the impact of decreased blood calcium (Ca) on subclinical endometritis; however, the highly dynamic and complex nature of the peripartum period in dairy cows may impair the establishment of the cause-and-effect relationship. The objective of this review is to compile information regarding hypocalcemia and subclinical endometritis and their relationship, as well as the available in vivo and in vitro study models that artificially induce subclinical states of hypocalcemia and endometritis in cows that are not in peripartum period. Regarding hypocalcemia, several studies have demonstrated the effectiveness and safety of protocols using Ca chelators such as ethylenediaminetetraacetic acid (EDTA) or ethylene glycol tetraacetic acid (EGTA) in vivo. The induced transitory hypocalcemia impaired feed intake, rumination and neutrophilic phagocytic and oxidative burst response. However, the effects on uterine environment remain poorly explored. Although these experimental models allow the understanding of the effects of hypocalcemia alone, without the peripartum metabolic and hormonal variations, the effects are likely underestimated because dairy cows may experience hypocalcemia for much longer periods. For studying bovine endometritis, the main experimental in vivo model is the intrauterine infusion of pathogenic bacteria or their components (lipopolysaccharide - LPS), which induce endometrial inflammation, even causing long-term negative effects. Several in vitro and ex vivo models have also been developed, which are mainly indicated to investigate the mechanisms underlying endometrial inflammation in cattle because there is no interaction with other tissues, organs and systems, as would occur in vivo. In conclusion, current models still face limitations and, therefore, future efforts to the development and refinement of in vivo and in vitro experimental models are necessary.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"23 1","pages":"e20240143"},"PeriodicalIF":2.1,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the addition of sulfated polysaccharides of animal and algal origin in the freezing medium of <i>Colossoma macropomum</i> (Characiformes: Serrasalmidae).","authors":"Carlos Henrique Sousa de Melo, Marcos Luiz da Silva Apoliano, Yara Silvino Sales, Renata Vieira do Nascimento, Vanessa Alves Pereira, Emanuel Martins da Costa, Jéssica Sales Lobato, José Ariévilo Gurgel Rodrigues, Carminda Sandra Brito Salmito-Vanderley","doi":"10.1590/1984-3143-AR2025-0105","DOIUrl":"10.1590/1984-3143-AR2025-0105","url":null,"abstract":"<p><p>The aim of this study was to evaluate the effects of supplementing the cryodiluent medium with sulfated polysaccharides (SP) extracted from marine algae (<i>Ascophyllum nodosum</i> or <i>Solieria filiformis</i>) and fish skin (<i>Colossoma macropomum</i>, <i>Prochilodus brevis</i>, or <i>Oreochromis niloticus</i>) on the cryopreservation of tambaqui semen. Twenty male tambaqui were used for semen collection and cryopreservation. For the fertilization assay, three males and five females were used. In Experiment 1, different concentrations of SP (0.0, 0.1, 0.25, 0.5, and 0.75 mg/mL) extracted from fish skin or marine algae were added to the freezing medium for <i>C. macropomum</i> semen. In Experiment 2, the results of sperm velocity analyses were used to select one concentration of each sulfated polysaccharide for use in fertilization trials. Among the treatments, <i>A. nodosum</i> at 0.75 mg/mL and <i>C. macropomum</i> at 0.50 mg/mL stood out, significantly improving sperm parameters such as motility, VCL, VSL, VAP, and LIN compared to the control group. <i>S. filiformis</i>, <i>P. brevis</i>, and <i>O. niloticus</i> also showed good results, with performance varying by concentration. Membrane integrity was higher in the algae-derived extract groups. Sperm morphology and DNA integrity did not differ significantly among groups. Fertilization rates remained high across all treatments (84.67% to 88.67%), with no statistically significant differences, indicating that the tested extracts did not compromise fertility. It was concluded that supplementation with SP from <i>A. nodosum</i> at 0.75 mg/mL and <i>C. macropomum</i> at 0.50 mg/mL, although all treatments showed similar fertility rates, is recommended as an additive to the semen dilution medium for tambaqui during freezing, as it improved important sperm parameters such as motility and VCL.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 4","pages":"e20250105"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12697337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protamine1, 2 and Catsper1: sperm quality and fertility indicators in Stallions.","authors":"Marília Marcolla de Figueiredo, Verônica La Cruz Bueno, Isabele Colla Lazzari Royes, Rodrigo Costa Mattos, Henrique Boll de Araujo Bastos, Sandra Fiala Rechsteiner","doi":"10.1590/1984-3143-AR2025-0040","DOIUrl":"10.1590/1984-3143-AR2025-0040","url":null,"abstract":"<p><p>The genes identification involved in male reproduction and the evaluation of its functions improve the comprehension about spermatogenesis molecular bases, fertilization, embryos early cleavage, spermatic quality and male infertility. The present study aimed to verify the Protamine1 <i>(PRM1),</i> Protamine2 <i>(PRM2)</i> and Cation Channel Sperm Associated 1 (<i>Catsper1)</i> genes expression into the equine sperm and their relations with the stallions' spermatic quality and fertility. Semen collections were performed in eighteen stallions, which were divided in two groups, based on fertility rates: fertile (with pregnancy rate per cycle ≥ 70%) and subfertile (with pregnancy rate per cycle ≤ 40%). The semen analysis was performed by Computer Assisted Sperm Analysis AndroVision®. The mRNA was extracted from the spermatozoa and the <i>PRM1</i>, <i>PRM2</i> and <i>Catsper1</i> gene expression verification in the spermatic cell was conducted by the qPCR technique. The results present a higher expression of <i>PRM1</i> and <i>Catsper1</i> in the fertile stallions' group than subfertile group; there was no correlation of <i>PRM1</i> and <i>PRM2</i> expression with spermatic quality parameters; there was correlation of the <i>Catsper1</i> expression with morphology and motility parameters. Negative correlation was found between the <i>PRM1/PRM2</i> ratio, fertility and motility parameters. The present research demonstrates that the <i>PRM1</i> and <i>Catsper1</i> genes are related to stallions' fertility and spermatic quality, and they may work as biomarkers.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 4","pages":"e20250040"},"PeriodicalIF":2.1,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12697338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of prior follicular wave synchronization and eCG on ovum pick-up and <i>in vitro</i> embryo production in Braford cows.","authors":"Felipe Gustavo Garcia, Bento Martins de Menezes, Caroline Fernandes Possebon, Rosana Klaus, Marcelo Silveira Albornoz, Janislene Mach Trentin, Daniele Missio, Daniela Dos Santos Brum, Fabio Gallas Leivas","doi":"10.1590/1984-3143-AR2025-0020","DOIUrl":"10.1590/1984-3143-AR2025-0020","url":null,"abstract":"<p><p>The aim of this study was to evaluate the effect of follicular wave synchronization and equine chorionic gonadotropin (eCG) prior to ovum pick-up (OPU) in Braford cows on the oocyte competence, maturation rate, and <i>in vitro</i> embryo production. Cows (n = 27) were divided into three groups on a crossover model: no treatment prior to OPU (Control), follicular wave synchronization (Synchro), and synchronization plus 800IU of eCG (eCG800). Donors of the groups Synchro and eCG800 were synchronized with 2 mg of estradiol benzoate (EB), prostaglandin F2α analogue (PGF2α) and intravaginal device with 1g of progesterone (P4) on D0. On day 3, eCG800 group donors received 800IU of eCG. On day 6, OPU was performed, and the number of follicles were counted and classified by diameter in small, medium, and large. In experiment 1, the viable oocytes were evaluated for competence development, nuclear maturation, and mitochondrial reorganization. In experiment 2, oocytes were matured, fertilized, and cultured <i>in vitro</i> to blastocyst stage. All analysis was performed by ANOVA, and the differences were compared by Tukey's test with significance <i>P</i> ≤ 0.05. The use of 800 IU of eCG increased (P < 0.05) the number of medium and large follicles compared to the Syncro group. The oocyte recovery, viability, nuclear or cytoplasmic maturation, cleavage, and grade 1 embryos rate did not differ among groups (P > 0.05). The blastocyst rate on D7 showed tendency (P = 0.075) to improve from Control (17±6.08%) to Synchro (23.8±8.95%) to eCG800 (37.3±6.51%). The dose of 800 IU of eCG 72 h before OPU increased the proportion and number of medium and large follicles in relation to the Control and Synchro groups, without affecting oocyte competence and tending to produce more blastocysts on D7.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 4","pages":"e20250020"},"PeriodicalIF":2.1,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12642421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can centrifugation improve cryotolerance of bovine embryos produced <i>in vitro</i>?","authors":"Danieli Aparecida Bóbbo Moreski, Josmar Mazucheli, Fabio Luiz Bim Cavalieri, Anthony Cesar de Souza Castilho, Anne Kemmer Souza, Camila Bortoliero Costa, Marcelo Marcondes Seneda, Isabele Picada Emanuelli","doi":"10.1590/1984-3143-AR2024-0123","DOIUrl":"10.1590/1984-3143-AR2024-0123","url":null,"abstract":"<p><p>We tested the effects of centrifuging in vitro matured bovine oocytes for varying times on embryo development and cryotolerance. The oocytes were divided into four groups: control (GC) and centrifuged groups [5433 x g: G5, n = 463 (5 min); G10, n = 461 (10 min); and G15, n = 483 (15 min)]. After centrifugation, the oocytes underwent <i>in vitro</i> fertilization for embryo production. Two parameters were evaluated: i) embryonic development (n = 1,878), and ii) cryotolerance evaluation (survival and hatching rates; n = 303). The CG and G10 groups showed blastocyst rates of 42.25% and 45.77%, respectively, higher than those of the other groups (p = 0.02). The hatching rate was equal (p > 0.05) in CG (91.96%), G5: (87.74%), and G10: (95.73%) groups; however, it was lower in G15: 77.06% (p < 0.01). In the CG group, 65.88% of cryopreserved embryos survived, which was different (p < 0.05) from that in G5 (82.02%) and G10 (82.28%) (p > 0.05). Post-freeze hatching percentage was 74.0%, 87.7%, and 47.7%, in G5, G10, and G15, respectively, which was significantly greater than that in CG (p < 0.01; 26.8%). Post-freeze hatching percentage in only G10 matched that of the non-cryopreserved embryos CG (p = 0.06, 92%). We conclude that oocyte centrifugation for 10 minutes was efficient for in vitro embryonic development and cryopreservation of cattle embryos.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 4","pages":"e20240123"},"PeriodicalIF":2.1,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12614880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145538684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}