Swine clones: potential application for animal production and animal models.

IF 1.6 4区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Pub Date : 2025-01-20 eCollection Date: 2025-01-01 DOI:10.1590/1984-3143-AR2024-0037

Thaís Naomi Gonçalves Nesiyama, Juliano Rodrigues Sangalli, Tiago Henrique Camara De Bem, Kaiana Recchia, Simone Maria Massami Kitamura Martins, André Furugen Cesar de Andrade, Juliana Germano Ferst, Gustavo Henrique Doná Rodrigues Almeida, Mariana Groke Marques, Renata Gebara Sampaio Dória, Adriano Bonfim Carregaro, Marcus Antônio Rossi Feliciano, Maria Angélica Miglino, Fabiana Fernandes Bressan, Felipe Perecin, Juliano Coelho da Silveira, Lawrence Charles Smith, Vilceu Bordignon, Flávio Vieira Meirelles

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引用次数: 0

Abstract

Somatic cell nuclear transfer (SCNT), or cloning, is used to reprogram cells and generate genetically identical embryos and animals. However, the cloning process is inefficient, limiting its application to producing valuable animals. In swine, cloning is mainly utilized to produce genetically modified animals. Indeed, recombinant DNA technologies have evolved considerably in recent years, with homologous recombination and gene editing technologies becoming more efficient and capable of recombining both alleles in a single cell. The selection of appropriate cells and their use as nuclear donors for SCNT is the most common method for generating edited and genetically modified animals for commercial and research purposes. This article reviews current applications of swine cloning and shares our personal experiences with the procedure in this species.

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猪克隆：用于动物生产和动物模型的潜在应用。

体细胞核移植（SCNT），或克隆，是用来重新编程细胞和产生基因相同的胚胎和动物。然而，克隆过程效率低下，限制了其用于生产有价值动物的应用。在猪方面，克隆主要用于生产转基因动物。事实上，近年来重组DNA技术已经有了很大的发展，同源重组和基因编辑技术变得更加高效，能够在单个细胞中重组两个等位基因。选择合适的细胞并将其用作SCNT的核供体是产生用于商业和研究目的的编辑和转基因动物的最常用方法。本文综述了目前猪克隆的应用现状，并分享了我们在猪克隆过程中的个人经验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Reproduction AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

2.30

自引率

11.80%

发文量

审稿时长

70 days

期刊介绍： Animal Reproduction (AR) publishes original scientific papers and invited literature reviews, in the form of Basic Research, Biotechnology, Applied Research and Review Articles, with the goal of contributing to a better understanding of phenomena related to animal reproduction. The scope of the journal applies to students, researchers and practitioners in the fields of veterinary, biology and animal science, also being of interest to practitioners of human medicine. Animal Reproduction Journal is the official organ of the Brazilian College of Animal Reproduction in Brazil.