{"title":"Computational and machine learning models for optimising germination under cold stress conditions of common bean with titania (TiO2) nanoparticles","authors":"Muhammad Aasim, Seyid Amjad Ali","doi":"10.1111/aab.70162","DOIUrl":"https://doi.org/10.1111/aab.70162","url":null,"abstract":"<p>Common bean (<i>Phaseolus vulgaris</i>) is an important edible legume that is sensitive to cold stress, especially at the germination and the seedling stage, leading to low germination and seedling vigour. Application of external stimulants can help to alleviate cold stress in plants at early stages. Titanium dioxide (TiO<sub>2</sub>) is one of the nanoparticles that can be used for this purpose. In this study, different concentrations of TiO<sub>2</sub> (0, 50, and 100 mg/L) were added to the Murashige and Skoog medium. Surface-sterilised seeds were cultured on the TiO<sub>2</sub>-enriched medium and transferred to 0 and 4°C for 120 and 240 h, then transferred to the growth room, automated at 24°C. The germination data were recorded daily and analysed using the GerminaR package in R. Seven different germination variables named germination percentage (GRP), mean germination time (MGT), mean germination rate (MGR), germination speed, uncertainty (UNC), synchronisation (SYN), coefficient of variation in germination time, seedling vigour, and coefficient of velocity of germination (CVG) were analysed by Taguchi design (TD) and response surface regression analysis (RSRA). Results of the TD revealed temperature, time, and TiO<sub>2</sub> as the most important factors based on the largest signal-to-noise ratio. RSRA revealed the impact of TiO<sub>2</sub>, temperature, and TiO<sub>2</sub> × time on GRP. TiO<sub>2</sub> × time was found to be important for MGT and CVG. The UNC factor was also regulated by temp., time, and temp. × times. The multi-output Random Forest model produced a mean cross-validated <i>R</i><sup>2</sup> of 0.718 across the germination metrics, with the highest values recorded for MGT (<i>R</i><sup>2</sup> = 0.872) and MGR (<i>R</i><sup>2</sup> = 0.755), while comparatively low <i>R</i><sup>2</sup> scores were recorded for SYN and CVG. This study allows us to understand the germination pattern for common beans under cold stress conditions and can be used for testing other crops.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"190 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149356474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pitambara, Ranjana Kumari, Prachi Singh, Shreya Sen, Shraddha Bhaskar Sawant, Yogesh M. Shukla, A. K. Singh, Debasis Mitra, Addisu Assefa
{"title":"Genetic mapping of quantitative trait loci associated with grain iron and zinc concentration in maize (Zea mays L.)","authors":"Pitambara, Ranjana Kumari, Prachi Singh, Shreya Sen, Shraddha Bhaskar Sawant, Yogesh M. Shukla, A. K. Singh, Debasis Mitra, Addisu Assefa","doi":"10.1111/aab.70166","DOIUrl":"https://doi.org/10.1111/aab.70166","url":null,"abstract":"<p>Iron (Fe) and Zinc (Zn) are essential micronutrients that play a crucial role in a wide range of metabolic processes in living organisms and are indispensable for maintaining normal growth, development and metabolic homeostasis. In the present study, a population of 120 recombinant inbred lines (RILs) was utilized for quantitative trait loci (QTL) analysis to assess grain iron (Fe) and zinc (Zn) content in maize across 2 growing seasons, using a total of 105 simple sequence repeat (SSR) markers. Composite interval mapping (CIM) performed using QTL Cartographer software identified one QTL for grain Fe content (qfe4.1) located on chromosome 4 with the nearest marker umc1390. For grain Zn content, three QTLs were detected: qzn4.1 (linked to umc1060), qzn4.2 (linked to umc1232) and qzn6.1 (linked to umc1753). Among these, qfe4.1 and qzn4.1 were consistently detected across both seasons, whereas qzn4.2 and qzn6.1 were identified in only one season and are therefore considered season-specific QTLs. The co-localization of qfe4.1 and qzn4.1 on chromosome 4 suggests that this genomic region may influence both Fe and Zn accumulation. However, further validation is required to confirm a common genetic basis. Overall, QTLs associated with grain Fe and Zn content were identified on chromosomes 4 and 6. These results provide a theoretical foundation for enhancing the iron and zinc content of maize lines through marker-assisted selection.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"190 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149356249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physiological evidence that Funneliformis mosseae combined with chitosan regulates the photosynthetic efficiency and endogenous hormones of Glycine max to promote plant growth","authors":"Feiyang Qi, Changlei Dai, Xianyou Xiong, Ruotong Wu, Xiaofeng Wang, Xiangyu Zhang, Zhuang Chen, Xingchen Du, Xianfeng Guo, Lin Li, Lili Jiang, Fuqiang Song, Yuqiang Wen","doi":"10.1111/aab.70163","DOIUrl":"https://doi.org/10.1111/aab.70163","url":null,"abstract":"<p>Soybean (<i>Glycine max</i> (L.) Merr.) production is often constrained by soil degradation and environmental stress. To explore green and growth-promoting pathways, this study systematically investigated the combined effects of inoculating arbuscular mycorrhizal fungi (<i>Funneliformis mosseae</i>, FM) and exogenous chitosan (CTS) on <i>G. max</i> growth, physiology, and photosynthetic efficiency. Results showed that the FM and CTS combined treatment (FM + CTS) promoted the aboveground fresh weight and yield in <i>G. max</i>, increasing by 65.04% and 12.67%, respectively, compared to the control group (CK). It also elevated the levels of endogenous hormones (CTK, IAA, SLs) in the leaves and enhanced the activity of antioxidant enzymes (CAT, SOD, POD). In terms of photosynthetic performance, the FM + CTS treatment group showed increases in net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (GH<sub>2</sub>O) by 43.39%, 120.61%, and 112.93%, respectively, compared to CK. Additionally, it improved the maximum photochemical efficiency of PSII (Fv/Fm) and the electron transport rate (ETR). The fitting of light response and CO<sub>2</sub> response curves showed that under FM + CTS treatment, the maximum net photosynthetic rate (Pnmax), maximum Rubisco carboxylation rate (Vcmax), and maximum electron transport rate (Jmax) all reached the highest levels. In summary, FM and CTS promoted <i>G. max</i> growth and development by regulating endogenous hormones, enhancing antioxidant defence capabilities, and optimizing the process of photosynthetic carbon assimilation. This provides systematic physiological evidence for elucidating the mechanisms of FM and CTS actions and lays a theoretical and practical foundation for developing a green <i>G. max</i> production technology system based on the combination of microbial and biostimulant treatments.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"190 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149355883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Incidence, geographic distribution, and molecular characterization of cucumber green mottle mosaic virus in cucurbits in Jordan","authors":"Areej Hussein, Nida' Salem, Paolo Margaria, Motasem Abumuslem, Akel Mansour, Barakat Abu-Irmaileh","doi":"10.1111/aab.70165","DOIUrl":"https://doi.org/10.1111/aab.70165","url":null,"abstract":"<p>Cucurbits are susceptible to infection by viruses belonging to different taxonomic groups, including cucumber green mottle mosaic virus (CGMMV, <i>Tobamovirus viridimaculae</i>), a highly stable and mechanically transmissible virus, also known to be seed-borne. This study aimed to investigate the incidence, distribution, and genetic diversity of CGMMV in cultivated and wild cucurbits, alternative hosts, and commercial cucurbit seeds in Jordan. A total of 700 samples from symptomatic and asymptomatic plants were collected in 48 fields across seven major cucurbit-growing regions. Screening by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) revealed CGMMV infection only in cucumber plants, with 63 positive samples collected in six fields located in three geographical regions, corresponding to 9% overall CGMMV infection. No CGMMV was detected in other cucurbit or weed samples. Additionally, six cucumbers and one melon seed lots tested positive for CGMMV by DAS-ELISA. Complementary reverse transcription-polymerase chain reaction analysis of 95 selected samples confirmed CGMMV infection in nine samples that had previously tested positive by ELISA. Biological assays using five CGMMV isolates showed typical symptoms on cucumber, with no evident phenotypic differences among the isolates, in accordance with the low diversity observed at the genome sequence level. Our findings provide additional insights into the phytosanitary status of cucurbits in Jordan and emphasise the need for continuous monitoring of seed health to support disease management.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"190 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149310840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lakshmi C. Nacey, Brian W. Garms, Jorgiane B. Parish, Judy Bellati, Nathan D. Luke, Peter Crisp
{"title":"Heat tolerance of Mediterranean land snails under induced dormancy in South Australia","authors":"Lakshmi C. Nacey, Brian W. Garms, Jorgiane B. Parish, Judy Bellati, Nathan D. Luke, Peter Crisp","doi":"10.1111/aab.70164","DOIUrl":"https://doi.org/10.1111/aab.70164","url":null,"abstract":"<p>Exotic pests associated with international trade are a major concern worldwide (Drake et al., 1989, <i>Biological invasions: A global perspective</i>; Campbell, 2001, <i>Bioscience</i>, 51:148–153). Invasive pests damage ecosystems, agriculture, forestry and the economy (Pimentel et al., 2001, <i>Agriculture, Ecosystems & Environment</i>, 84:1–20). The current option to treat exotic molluscs at Australian borders is methyl bromide fumigation (128 ppm [g/m<sup>3</sup>], 24 h). Dry heat treatment trials have been conducted with different land snail species but there is a lack of research on temperatures and exposure periods that effectively kill the most heat tolerant species of land snails at their most heat tolerant life stage. Elevated temperatures are predicted to efficiently kill Mediterranean snail species <i>Cernuella virgata</i> (Da Costa, 1778) and <i>Theba pisana</i> (Müller, 1774), but lethal temperatures and exposure period combinations are not known for these snails, particularly at different development stages and during dormancy. The aim of this study was to determine the temperature and exposure duration required to induce mortality in the heat tolerant snail species <i>C. virgata</i> and <i>T. pisana.</i> We hypothesise that snails held dormant in tins for longer periods exhibit higher thermal tolerance, requiring longer heat exposure for mortality. Snails were collected, loaded into tins 2, 4 and 6 weeks prior to treatment and kept undisturbed without food and water to induce dormancy. Tins containing snails were exposed to 50°C, 55°C and 60°C temperatures for exposure periods at intervals between 15 and 180 min, with controls kept at ambient temperature. <i>T. pisana</i> were able to survive for 3 h at 50°C and 2 h at 55°C. Heat treatment at 60°C for 1 h was found to control both snail species tested. A significant relationship was found between longer dormancy and heat tolerance when <i>T. pisana</i> were treated at all temperatures, particularly at 55°C (<i>p</i> < .001). The results from this project support the development of a scientifically based dry heat treatment protocol to effectively control the most heat tolerant species of invasive snails intercepted during international trade.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"190 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70164","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149310842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aranzazu Moreno, Inmaculada Ferriol, Lucia Zurita, Maria Plaza, Rocio Galán-Cubero, Adriana Urbano-Bernabé, Alberto Fereres, Fernando Ponz
{"title":"A nanobiotechnological approach to control non-persistent aphid-transmitted plant viruses, based on functionalized virus-like particles","authors":"Aranzazu Moreno, Inmaculada Ferriol, Lucia Zurita, Maria Plaza, Rocio Galán-Cubero, Adriana Urbano-Bernabé, Alberto Fereres, Fernando Ponz","doi":"10.1111/aab.70161","DOIUrl":"https://doi.org/10.1111/aab.70161","url":null,"abstract":"<p>Control of non-persistently-aphid transmitted plant viruses is based on preventive measures such as physical or photoselective barriers or reflective mulches as well as host plant resistance. However, these methods are not always durable and effective and are difficult to implement in the field because transmission occurs in a matter of seconds when aphids land and immediately probe on a plant. Currently, there are no available strategies to disrupt the transmission process of non-persistently-transmitted viruses based on virus–vector molecular interactions. In this work, we engineered a potyvirus-derived virus-like nanoparticle (VLP) to interfere in the transmission of the potyviruses turnip mosaic virus (TuMV) and zucchini yellow mosaic virus (ZYMV) by their aphid vectors. Using the engineered construct, we found that there was also interference with the transmission of the cucumovirus, cucumber mosaic virus (CMV). This nanoparticle was a fusion of a modified version of the TuMV helper component protease (HC-Pro) and the capsid protein (CP). This construct was delivered to aphids through transient expression in <i>Nicotiana benthamiana</i> plants, followed by aphid acquisition. Retention of the engineered construct within the aphid stylet cuticle before virus acquisition reduced cucumovirus and potyvirus transmission rates by 38%–56%, respectively. This novel biotechnological approach targets the interference of non-persistent, aphid-mediated virus transmission. The genetic construct is adaptable for stable integration into transgenic plants or for direct spraying onto plants using appropriate surfactants or wetting agents, and therefore represents an effective strategy for the control of vector-borne viruses.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149146418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tatiane Oliveira Santos, Josué Pinheiro Machado, Wesley Lopes Vilela, Raissa Castro Nunes, Carlos de Alfredo Lopes de Carvalho, Emerson Dechechi Chambó
{"title":"Autonomous self-pollination provides reproductive assurance, whereas insect pollination enhances yield in sesame under tropical field conditions","authors":"Tatiane Oliveira Santos, Josué Pinheiro Machado, Wesley Lopes Vilela, Raissa Castro Nunes, Carlos de Alfredo Lopes de Carvalho, Emerson Dechechi Chambó","doi":"10.1111/aab.70159","DOIUrl":"https://doi.org/10.1111/aab.70159","url":null,"abstract":"<p>Sesame (<i>Sesamum indicum</i> L.) exhibits a mixed mating system, in which both self- and cross-pollination can occur. In tropical environments, the contribution of pollinators may enhance fertilization efficiency and increase crop yield. This study aimed to evaluate the role of pollinators in sesame production and its degree of pollinator dependence. Two experiments were carried out in Cruz das Almas, Bahia, Brazil. In the first, open-pollinated plants were compared with pollinator-excluded plants (cages) to assess grain yield, yield components and seed quality. In the second, pollination requirements were investigated using five treatments: open pollination, pollinator exclusion (paper bags), wind-mediated pollination (cages), geitonogamous pollination and hand cross-pollination. Open-pollinated plants produced a greater number of fruits and seeds per fruit, resulting in greater grain yield. In contrast, seed mass, number of branches and physiological seed quality were not affected by pollination treatments. Seedling dry biomass was greater in the presence of pollinators. Overall, grain yield was 28.6% greater under open pollination, corresponding to a pollinator dependence of 22%. Controlled pollination treatments showed that self-pollination provides reproductive assurance under pollinator limitation, whereas insect pollination enhances reproductive performance. Sesame exhibited a mixed mating system, with self-pollination and insect-mediated pollination acting complementarily. This balance helps ensure stable production, while pollinators play an important role in maximizing yield under tropical agricultural conditions.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149145997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jessica M. M. Adams, Maria Jose Pilar Martinez-Martin, Catrin Angharad Evans, Ruth Sanderson, Irene Griffiths, Catherine J. Howarth
{"title":"Relative variation in β-glucan components in the oat grain (Avena sativa) during development and between varieties","authors":"Jessica M. M. Adams, Maria Jose Pilar Martinez-Martin, Catrin Angharad Evans, Ruth Sanderson, Irene Griffiths, Catherine J. Howarth","doi":"10.1111/aab.70160","DOIUrl":"https://doi.org/10.1111/aab.70160","url":null,"abstract":"<p>Oat β-glucan provides health-benefitting properties, with the ratio of subunit trimers to tetramers the main identified molecular difference between this and other grain-derived β-glucans. This ratio change causes alterations in the viscosity and gelling potential of the β-glucan, which in turn affects cholesterol uptake and conversion. This study compares both the physical variables (grain dry weight, width, length) and the ratio and total yield of trimer and tetramer units in developing winter oat (Mascani) grain over two harvest years. Results found the grain weights and widths correlated through the development stages, with length increasing earlier in the development, matching developmental studies in wheat grains. Within the total β-glucan fraction, the yield followed the trend for grain weight, with the β-glucan ratio between the composite subunits highly conserved from the beginning of grain formation. β-Glucan yields were shown not to differ between grains taken from locations across the whorls of the panicle or between the grain types (primary or secondary) within the spikelets, showing composition consistency despite developmental differences. To widen the significance of this work, the yields and ratios of β-glucan trimer and tetramer were also assessed in 46 different mature oat grain varieties (excluding Mascani). These were found to have a reduced trimer:tetramer ratio correlation (<i>R</i><sup>2</sup> = 0.84; in Mascani-only studies <i>R</i><sup>2</sup> = 0.99–1), showing limited diversity between accessions. All spring oat varieties had a lower trimer:tetramer ratio than seen in the Mascani grain initial studies, a positive breeding attribute. A further correlation was seen between trimer:tetramer ratios, grain size and nitrogen, with lower β-glucan ratios associated with larger grains and lower nitrogen content. Collectively, findings from this study identify useful trait variation for future breeding programme selection considering enhanced health benefits.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149145998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cristiane J. Da-Silva, Cristian E. Collado, Jan van Butsele, Ricardo Hernández
{"title":"Optimizing light spectra for soybean cultivation in controlled environments: Effects on plant growth, flowering, seed production, and seed quality","authors":"Cristiane J. Da-Silva, Cristian E. Collado, Jan van Butsele, Ricardo Hernández","doi":"10.1111/aab.70158","DOIUrl":"https://doi.org/10.1111/aab.70158","url":null,"abstract":"<p>Soybeans are essential for global agriculture as a major source of protein and oil, supporting food security, livestock feed, and renewable energy industries worldwide. Manipulating the light spectrum using LEDs presents a unique opportunity to optimize soybean growth in a controlled environment. In this study, we aimed to grow compact soybeans, accelerate flowering, increase seed production, and evaluate physiological variables. Two soybean varieties (CZ 75 70LL and S16-14801C) were cultivated from seeds in controlled conditions (27°C/26°C, day/night; 68% relative humidity; 590 μmol mol<sup>−1</sup> CO<sub>2</sub>) in 11 L plastic pots containing peat-moss substrate. A week after germination, the plants were exposed to one of four light-spectrum treatments at a photon flux density of 700 μmol m<sup>−2</sup> s<sup>−1</sup>. These treatments had different percent photon flux ratios of blue (B: 400–500 nm), green (G: 500–600 nm), red (R: 600–700 nm), and far-red (FR: 700–750 nm) wavelengths: (1) 22B:50G:26R:2FR (White; control), (2) 20B:80R, (3) 50B:50R, and (4) 40B:40R:20FR. Both varieties across all treatments reached the harvest stage at 121 ± 4 days, with all flowers being open and producing light-yellow pollen. Higher levels of blue light in 50B:50R increased leaf transpiration, thereby reducing water-use efficiency and photosynthetic efficiency. Seed yield assessments indicated that the 40B:40R:20FR treatment increased seed production, rising to 34% in seed number and 37% in seed weight, compared with the white control treatment. Morphological evaluations indicated that plants under 50B:50R and 40B:40R:20FR were 1.7–2.4 and 1.3–1.5-fold shorter than plants grown under the white treatment. These findings confirm that the light spectrum can be tuned to reach specific objectives and optimize soybean growth and development in controlled environments.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theresa Gerber, Kato Van Ruymbeke, Lionel Alletto, Gaëtan Seimandi-Corda, Erik Mathijs
{"title":"A framework for integrated assessment of cereal–legume intercropping: Linking environmental impacts to farm-level economic performance","authors":"Theresa Gerber, Kato Van Ruymbeke, Lionel Alletto, Gaëtan Seimandi-Corda, Erik Mathijs","doi":"10.1111/aab.70153","DOIUrl":"https://doi.org/10.1111/aab.70153","url":null,"abstract":"<p>Intensive European cropping systems face increasing environmental pressures, yet agronomic and environmental performance of different cropping systems is often evaluated separately, limiting the understanding of the true feasibility of ecologically intensive systems. To bridge this gap, we develop a framework that integrates agronomic and environmental data, using data from field experiments to demonstrate its application using French cereal–legume trials to assess the environmentally adjusted gross margins and the implications for farmer decision-making. This proof-of-concept showed the sole cropping system remained more economically viable than the intercropped system even when accounting for externalities. However, the analysis also demonstrated that data limitations, methodological assumptions, and uncertainties in environmental valuation constrain the interpretability of results, underscoring the need for more comprehensive and coordinated experimental designs in future applications of the environmentally adjusted gross margin framework.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}