Annals of Applied Biology最新文献

{"title":"Spider communities in the aboveground oak forests of central Europe are determined by vertical scale: An important missing link for biodiversity conservation","authors":"Jana Niedobová,&nbsp;Vladimír Hula,&nbsp;Jiří Foit","doi":"10.1111/aab.12966","DOIUrl":"https://doi.org/10.1111/aab.12966","url":null,"abstract":"<p>Spiders are generalist predators able to respond very well to environmental conditions. Therefore, they are good bioindicators for forest management practices. The vertical distribution of spiders in forest stands has very rarely been studied. Thus, we investigated patterns in species richness, diversity, life-history traits and functional diversity of spiders within the aboveground vertical stratification of oak dominated forests and their response to the canopy cover gradient. There were three localities sampled during four growing seasons (2008–2011, Southern Moravia, Czech Republic). Spiders were sampled monthly by flight intercept traps, which were placed in three stratigraphical levels of oak stands. Altogether, 3592 spiders, representing 18 families, and 112 distinguishable species were collected during the study. We found that species richness, total abundance the abundance of ambush hunters, other hunters and scarce, rare and very rare species of spiders increased from ground level to the canopy, and that species composition changed across the vertical gradient. Some of these characteristics were significantly influenced by interaction with canopy cover. Less common ballooning species preferred the ground part of forest stratification. Canopy cover evaluation showed that the abundance of orb web weavers and less common ballooning species was highest in the more closed canopies. The opposite trend was found for sheet web weavers, very abundant species and open habitat species. Space web weavers, other hunters and very common ballooning species showed increased abundance in the higher layers under conditions of more open canopy cover. Our results suggest that sampling spiders across the vertical gradient of forest stands might be important for a proper biodiversity and management practice evaluation, although most recent assessments were conducted on ground-dwelling spiders obtained from pitfall traps.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"288-301"},"PeriodicalIF":2.2,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12966","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterisation of Zucchini yellow fleck virus and a novel Nepovirus from next-generation sequencing of mixed virus infections in cucumbers (Cucumis sativus) from Crete","authors":"Anthony James,&nbsp;Nikoleta Kryovrysanaki,&nbsp;Christos Andronis,&nbsp;Polyxeni G. Pappi,&nbsp;Kriton Kalantidis,&nbsp;Konstantina Katsarou","doi":"10.1111/aab.12962","DOIUrl":"https://doi.org/10.1111/aab.12962","url":null,"abstract":"<p>Cucumbers are susceptible to infections with many characterised virus species. In some cases, mixed virus infections occur and produce novel symptoms. In Greece, routine screening is carried out when virus infection is suspected, or novel symptoms are observed in the field. To identify the viruses associated with distinct symptoms observed in samples from commercial cucumber production areas on the island of Crete, Greece, we carried out high-throughput sequencing (HTS) from a pool of six samples. Following assembly and BLAST analysis, we identified at least seven viruses based on similarity to published sequences. Two of these sequences represented novel, near-complete genomes of a putative new nepovirus and of zucchini yellow fleck virus (ZYFV). To confirm the HTS results, the six samples were screened for all identified viruses, and their presence was confirmed through Sanger sequencing of PCR products. The full-length genomes of both the nepovirus and ZYFV were amplified by PCR and confirmed by Sanger sequencing. We have generated the complete genome of a novel nepovirus from cucumber as well as the first complete genome sequence of a cucumber-infecting ZYFV isolate from Crete. The nepovirus was mechanically transmissible to <i>Nicotiana benthamiana</i> and induced typical cytopathological modifications consistent with virus infection, as revealed by TEM studies. We propose to name this new virus Cucumber nepovirus A (CuNVA).</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"248-261"},"PeriodicalIF":2.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12962","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic variations in diverse accessions of linseed (Linum usitatissimum L.) germplasm under PEG-induced water stress and its implications for drought tolerance","authors":"Sheela,&nbsp;Vikender Kaur,&nbsp;Shashank Kumar Yadav,&nbsp;Munisha Kheralia,&nbsp;Manoj Kumar","doi":"10.1111/aab.12963","DOIUrl":"https://doi.org/10.1111/aab.12963","url":null,"abstract":"<p>Water constraint is a crucial factor in determining the productivity and production of linseed or flaxseed globally. The root system of flaxseed consists of a single taproot mainly confined to the topsoil; thus, the structure of the root system significantly impacts the uptake of water from the soil. This research conducted root–shoot phenotyping on a diverse range of linseed germplasm under PEG-induced water deficit stress conditions to unravel the potential of genebank germplasm for drought tolerance. Varied responses to stress and substantial diversity in genotypic response were observed among the linseed accessions for all growth parameters under both normal and stress conditions. The application of PEG led to a decrease (ranging from 7.39% to 62.02%) in all parameters except chlorophyll content, which exhibited an increase of 13.76% in response to stress conditions. Principal component analysis revealed that the first four principal components (PCs) with Eigenvalue &gt;1 explained 74.23% of the total variance, with the first PC alone accounting for 42.15% of the total variance contributed by various traits such as leaf width, shoot length, root–shoot biomass, root length, surface area, and volume. Assessing the comparative performance based on the stress susceptibility index (SSI) for shoot–root length and root surface area, a subset of 12 drought tolerant (SSI ≤ 0.50) and 5 susceptible (SSI &gt; 1.00), genotypes was constituted for validation at the adult plant stage. The accessions IC0096648, IC0523799, IC0249015, IC0096587, IC0385336, IC0498744, IC0499170, EC0041481, IC0526017, IC0623723, IC0113110, and IC0621685 exhibiting tolerance to PEG-induced water stress during the initial (seedling stage) growth maintained physiological efficiency and yield at the adult plant stage. The elite drought-tolerant genotypes identified in the present study will provide access to genetically diverse material in breeding to enhance drought tolerance in linseed.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"262-278"},"PeriodicalIF":2.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity, genotyping and evolutionary history of the global population of Orthotospovirus tomatomaculae genomic segments","authors":"Majid Siampour,&nbsp;Sajedeh Noorbakhsh,&nbsp;Keramatollah Izadpanah","doi":"10.1111/aab.12960","DOIUrl":"https://doi.org/10.1111/aab.12960","url":null,"abstract":"<p>Tomato spotted wilt virus (<i>Orthotospovirus tomatomaculae</i>; TSWV) is a destructive pathogen that affects over 1000 plant species worldwide. To elucidate its evolutionary mechanisms, genetic diversity and emergence timeline, we examined around 100 TSWV isolates with complete genome sequences available in GenBank. This study also included the complete genome of a TSWV isolate, IRP4, which has recently been implicated in an outbreak affecting greenhouse-grown bell peppers in Iran. Pairwise genetic divergence analysis revealed varying levels of genetic differences among TSWV RNA segments. Phylogenetic and sequence analysis determined that the global TSWV population consists of three major groups based on each of the three RNA segments (L, M, and S). Results suggested multiple introductions of the virus into different regions, indicating that the geographical origin of the isolates is not the sole factor determining their phylogenetic grouping. The large incongruences observed in the phylogeny of the TSWV segments and the analysis of genetic diversity patterns highlight extensive reassortment events in TSWV. As a result, 11 genotypes were identified within the TSWV population. The Iranian IRP4 isolate, along with nine other isolates from Europe, Asia, and South America shared a common genotype designated L3-M1-S1. This suggests that this potentially reassortant genotype has undergone long-distance intercontinental movement. Similar to IRP4, the majority of TSWV isolates with the L3-M1-S1 genotype were also obtained from pepper, suggesting a potential role of the host plant in the evolution of this genotype. Furthermore, temporal analysis suggests that the existing population of TSWV, circulating between 1996 and 2023, originated from a common ancestor that existed 107–284 years ago. The population size of TSWV experienced a significant expansion for 10–15 years since 1980 and then remained constant until recently.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"232-247"},"PeriodicalIF":2.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraspecific competition and performance of Anagrus virlai parasitizing the corn leafhopper","authors":"Jorge G. Hill,&nbsp;María V. Coll-Aráoz,&nbsp;Erica Luft-Albarracin,&nbsp;Patricia C. Fernández,&nbsp;Eduardo G. Virla","doi":"10.1111/aab.12965","DOIUrl":"https://doi.org/10.1111/aab.12965","url":null,"abstract":"<p>Intraspecific competition and avoidance of superparasitism are critical for biological control. In this study, we conducted behavioural and biological trials to assess intraspecific competition in the egg parasitoid <i>Anagrus virlai</i>, targeting the corn leafhopper <i>Dalbulus maidis</i>. Firstly, we investigated whether <i>A. virlai</i> can distinguish between unparasitized and parasitized host eggs using long-range cues in an olfactometer experiment. Secondly, we evaluated whether <i>A. virlai</i> parasitism and emergence under varying host densities are affected by the presence of multiple conspecific wasps foraging within the same host patch. In the olfactometer test, <i>A. virlai</i> did not differentiate between corn leaves with parasitized and unparasitized eggs. Regarding the parasitoid's performance, the number and percentage of parasitized eggs were influenced by the density of females foraging simultaneously in the same host patch. Significant increases in collective parasitism were observed when two or more parasitoids were present in each arena. Competitive interaction did not affect wasp emergence, and <i>A. virlai</i> proved to be a solitary species. Moreover, the negative relationship between per-capita parasitism and the number of conspecific wasps per arena demonstrated mutual interference among parasitoids. These findings highlight the impact of competitive interactions on <i>A. virlai</i> performance and suggest a potential aggregated response of the parasitoid to high-density patches of hosts.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"279-287"},"PeriodicalIF":2.2,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere regulation: Development and blueprint for soil-borne disease suppression in strawberry","authors":"Tao Kang,&nbsp;Miaomiao Zhang,&nbsp;Mingyang Xia,&nbsp;Kun Chen,&nbsp;Yufang Zhai,&nbsp;Baobao Yan,&nbsp;Yupeng Wang,&nbsp;Hongmiao Wu","doi":"10.1111/aab.12961","DOIUrl":"https://doi.org/10.1111/aab.12961","url":null,"abstract":"<p>Strawberry is an important economic crop in China, but it is seriously impacted by soil-borne diseases. In recent years, the intensification and monoculture of strawberry planting have exacerbated the occurrence of diseases, which pose a serious threat to the development of the strawberry industry. This study focused on the current research on strawberry major fungal, bacterial, and viral diseases and insect pests that occur during strawberry cultivation. The potential roles of ecological prevention and control strategies in alleviating strawberry diseases, such as high-temperature soil solarization, chemical fumigation, reductive soil disinfestation, diversified crop cultivation, biochar amendment, wormcast improvement, and synthetic microbial community improvement, were analyzed. This study highlighted that research should focus on the rhizosphere ecology perspective and healthy soil, screening highly efficient stress-resistant, disease-resistant, and growth-promoting microorganisms and constructing a functionally complementary and stable synthetic community. The collaborative efficiency of healthy soil in promoting cooperation between exogenous and indigenous microorganisms should be comprehensively studied. Furthermore, a variety of strategies that combine rhizosphere regulation to alleviate the continuous cropping obstacles in strawberries should be adopted, aiming to provide references for the ecological prevention and control of diseases in crops and the high-quality development of the industry.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 1","pages":"27-37"},"PeriodicalIF":2.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating multi-trait genomic selection with simulation strategies to improve grain yield and parental line selection in rice","authors":"Chandrappa Anilkumar,&nbsp;Rameswar Prasad Sah,&nbsp;T. P. Muhammed Azharudheen,&nbsp;Sasmita Behera,&nbsp;Soumya Priyadarshini Mohanty,&nbsp;Annamalai Anandan,&nbsp;Bishnu Charan Marndi,&nbsp;Sanghamitra Samantaray","doi":"10.1111/aab.12964","DOIUrl":"https://doi.org/10.1111/aab.12964","url":null,"abstract":"<p>Inclusion of correlated secondary traits in the prediction of primary trait in multi-trait genomic selection (GS) models can improve the predictive ability. Our objectives in the present investigations were to (i) evaluate the effectiveness of multi-trait and single-trait GS models for the higher predictive ability and (ii) compare the breeding potential of parental lines selected based on phenotype and GS for grain yield in rice. We used phenotype data of five correlated traits as secondary traits evaluated to predict the grain yield, a primary trait. Yield related functional markers were used for prediction. Breeding populations were simulated using the best parents selected through GS and phenotype based selection. Results suggest that the multi-trait model resulted in higher predictive abilities (0.82 for grain yield) than single-trait models (0.76 for grain yield) and parents selected through GS have potential to produce superior progenies. We conclude that the use of a multi-trait GS approach is advantageous over single-trait models, and the GS also help selecting potential parents for developing improved populations. The results of the study have potential scope for improving quantitative traits using GS in rice.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 2","pages":"216-227"},"PeriodicalIF":2.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plum pox virus: An overview of the potyvirus behind sharka, a harmful stone fruit disease","authors":"Juan Antonio García,&nbsp;Bernardo Rodamilans,&nbsp;Sandra Martínez-Turiño,&nbsp;Adrian A. Valli,&nbsp;Carmen Simón-Mateo,&nbsp;Mariano Cambra","doi":"10.1111/aab.12958","DOIUrl":"https://doi.org/10.1111/aab.12958","url":null,"abstract":"<p>The study of <i>Potyvirus plumpoxi</i> (plum pox virus, PPV) has a long history, beginning with its foundational description by Atanassof in 1932 in Bulgaria. Interest in the virus has significantly increased over the past decades, as evidenced by a rise in published articles, highlighting its importance in plant pathology. Research on PPV offers broader insights into the biology and pathology of the extensive <i>Potyviridae</i> virus family to which it belongs. The virus's large strain diversity and wide host range make it a key subject for research into plant–virus interactions, host adaptation and virus evolution. PPV causes sharka, the most damaging disease affecting stone fruit trees. Economic losses from sharka disease have recently been estimated at over €2.4 thousand million for the last 28 years. The significance of this disease is further amplified by the ease of aphid-mediated transmission from infected plant material and the virus's ability to establish and spread to new regions, making it a global agricultural challenge. This review aims to provide a comprehensive overview of the various biological traits of PPV and will form the basis of an updated version on the Association of Applied Biologists Description of Plant Viruses for PPV.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 1","pages":"49-75"},"PeriodicalIF":2.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12958","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative biochemical and transcriptomic analyses reveal the bases of Rhopalosiphum padi L. resistance in Aegilops tauschii Coss.","authors":"Deepika Narang,&nbsp;Beant Singh,&nbsp;Satvir Kaur Grewal,&nbsp;Satinder Kaur,&nbsp;Parveen Chhuneja","doi":"10.1111/aab.12950","DOIUrl":"https://doi.org/10.1111/aab.12950","url":null,"abstract":"<p><i>Rhopalosiphum padi</i> L. is one of the most devastating cereal aphids in the world. Its feeding does not induce a clear phytotoxic response in plants. Little information is available on defence response triggered by <i>R. padi</i> feeding on wild wheats, frequently used to improve hexaploid wheat. An attempt has been made to understand the differential biochemical and molecular responses of <i>R. padi</i> feeding on moderately resistant and susceptible <i>Aegilops tauschii</i> genotypes. RNA-Seq based transcriptomic analysis was conducted on 14 day-old leaf tissues of moderately aphid resistant (R) genotype (<i>Ae. tauschii</i> pau14232) and susceptible (S) genotype (<i>Ae. tauschii</i> pau14138) infested with <i>R. padi</i> for 12, 24 and 48 h. Plant response to aphid infestation was comparatively faster in R genotype. A total of 10,589 genes were altered in R genotype in comparison to only 8068 differentially expressed genes (DEGs) in S genotypes. There was a gradual increase in significant DEGs from 12 to 48 h after <i>R. padi</i> infestation in R (4931–6582) and S genotype (4860–5736). Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that 18 pathway genes were differentially expressed in R and S genotypes at different feeding time intervals. Gene ontology enrichment analysis of DEGs after <i>R. padi</i> feeding indicated up-regulation of genes for secondary metabolite synthesis, reactive oxygen species (ROS)-scavenging, transcription factors (ethylene responsive transcript factor [ERF], NAC, WRKY, MADS-box and Myb) and salicylic acid and ethylene signalling pathways in R genotype while down-regulation in S genotype. However, photosynthesis and light-harvesting DEGs were down-regulated in both the genotypes resulting in decreased chlorophyll content. Biochemical analysis showed that <i>R. padi</i> feeding induced substantial hydrogen peroxide accumulation in R genotype unlike in S genotype. Also, activities of peroxidase and catalase were comparatively higher in R than S genotype, confirming efficient ROS-scavenging in R genotype. Quantitative polymerase chain reaction expression validation studies of 11 DEGs followed same trend as in RNA-Seq data. Our study concluded up-regulation of stress response genes, salicylate and ethylene signalling pathways and efficient ROS-scavenging imparted a fitness advantage to R genotype during <i>R. padi</i> attack, resulting in reduced nymphiposition and nymphal survival. These findings will help in understanding the mechanism of host plant resistance and the development of aphid resistant wheat varieties.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 2","pages":"168-180"},"PeriodicalIF":2.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nectar-inhabiting bacteria differently affect the longevity of co-occurring egg parasitoid species by modifying nectar chemistry","authors":"Evgenia Sarakatsani,&nbsp;Jay Darryl L. Ermio,&nbsp;Shahinoor Rahman,&nbsp;Patrizia Bella,&nbsp;Alfonso Agrò,&nbsp;Mirella Lo Pinto,&nbsp;Ezio Peri,&nbsp;Stefano Colazza,&nbsp;Bart Lievens,&nbsp;Michael Rostás,&nbsp;Antonino Cusumano","doi":"10.1111/aab.12959","DOIUrl":"https://doi.org/10.1111/aab.12959","url":null,"abstract":"<p>Flowering plants can be introduced in modern agroecosystems to support resident natural enemies in the context of Conservation Biological Control (CBC). Buckwheat (<i>Fagopyrum esculentum</i>) (Polygonales: Polygonaceae) has been shown to enhance the longevity of several parasitoids through the provision of high quality and easily accessible floral nectar. Yet floral nectar is ubiquitously colonized by microbes which can change nectar chemistry with consequences for parasitoids. Nonetheless, how bacteria associated with buckwheat floral nectar affect parasitoid performance is not known. In this study, adult females of <i>Trissolcus basalis</i> (Hymenoptera: Scelionidae) and <i>Ooencyrtus telenomicida</i> (Hymenoptera: Encyrtidae), two parasitoids of <i>Nezara viridula</i> (Hemiptera: Pentatomidae), were provided with synthetic nectar fermented by 14 bacterial isolates originating from buckwheat nectar. We recorded the effect of bacterial fermentation on female longevity and nectar chemistry. In the case of <i>T. basalis</i>, females consuming nectar fermented by <i>Bacillus</i> sp., <i>Brevibacillus</i> sp., <i>Brevibacterium frigoritolerans</i>, <i>Saccharibacillus endophyticus</i>, and <i>Terribacillus saccharophilus</i> significantly enhanced their longevity compared with females fed with non-fermented nectar. For <i>O. telenomicida</i>, enhanced longevity was recorded only in the case of <i>B. frigoritolerans</i> and <i>Pantoea dispersa</i>. For both parasitoids, no negative effects due to bacterial fermentation of nectar were recorded. Chemical investigations of bacteria-fermented nectars revealed an increased diversity in the composition of sugars and sugar alcohols, whereas non-fermented nectar only contained sucrose. Our findings show that nectar-inhabiting bacteria are important “hidden players” in the interactions between flowers and parasitoids, an indication that a better understanding of plant–microbe–insect interactions could improve CBC programmes.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 2","pages":"204-215"},"PeriodicalIF":2.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12959","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}