Annals of Applied Biology最新文献

{"title":"Squash silverleaf disorder and whitefly (Bemisia tabaci MEAM1) infestation: Implications for plant disease management in a changing climate","authors":"Nihal Oztolan-Erol,&nbsp;Yusuf Sen,&nbsp;Melike Cokol-Cakmak,&nbsp;Guler Demirbas-Uzel,&nbsp;Stuart J. Lucas","doi":"10.1111/aab.70150","DOIUrl":"https://doi.org/10.1111/aab.70150","url":null,"abstract":"<p>Climate warming over the next century is expected to have a large impact on the interactions between pests and their hosts in agriculture. Vector-borne diseases are sensitive to warming or climate change because temperature changes can alter vector development rates, shift their geographical distribution, or alter transmission dynamics. One clear recent example of this is squash silverleaf disorder (SLD), a physiological disease of cucurbits resulting from feeding by <i>Bemisia tabaci</i> MEAM1 (silverleaf whitefly, SLW). SLD is marked by silvering of the leaves of <i>Cucurbita</i> spp. resulting in decreased photosynthetic efficiency, loss of vigour, and thus declining fruit production. When SLW infestation occurs in combination with drought stress, the severity of SLD dramatically increases. In this review, we summarize the pathology of SLD on crops, the available studies both phenotypically and genetically characterizing SLD in <i>Cucurbita</i> spp., and also the available SLD management strategies. Finally, we identify major research questions that remain to be answered in order to ameliorate the increasing challenge that this disorder presents to sustainable vegetable crop production.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dispersal responses of cereal aphids to wheat cultivar and planting configuration","authors":"Maria Elisa D. A. Leandro,&nbsp;Joe M. Roberts,&nbsp;Edward T. Dickin,&nbsp;Tom W. Pope","doi":"10.1111/aab.70152","DOIUrl":"https://doi.org/10.1111/aab.70152","url":null,"abstract":"<p>Cultivar mixtures are advocated as an agroecological strategy for suppressing cereal aphid vectors and reducing barley yellow dwarf virus (BYDV) transmission, yet their effects on within-crop dispersal remain largely untested, particularly across vector species and virus infection states. We quantified the walking dispersal of <i>Sitobion avenae</i> and both BYDV-PAV-viruliferous and non-viruliferous <i>Rhopalosiphum padi</i> through wheat stands comprising a 1970s cultivar (Maris Huntsman) and a <i>Bdv2</i>-carrying BYDV-resistant cultivar (RGT Wolverine), arranged as monocultures, alternating-row intercropping and randomised mixtures. Dispersal responses were species-specific, cultivar-dependent and fundamentally altered by virus infection status. <i>S. avenae</i> colonised non-source plants at comparable rates across all treatments. Non-viruliferous <i>R. padi</i> showed strong cultivar-mediated responses as colonisation on Maris Huntsman monocultures was 67%–69% lower than on Wolverine, with a steep distance-decay gradient (64% decline per unit distance) indicating rapid arrestment on the preferred host. Within randomised mixtures, <i>R. padi</i> preferentially colonised Maris Huntsman, but the presence of Wolverine plants disrupted arrestment and produced spatially uniform dispersal resembling that on Wolverine monocultures. Source plant cultivar, rather than surrounding planting configuration, was the primary driver of <i>R. padi</i> dispersal in mixtures. Critically, BYDV-PAV infection eliminated all cultivar-specific responses in <i>R. padi</i> as viruliferous aphids dispersed uniformly regardless of cultivar arrangement, consistent with virus-induced behavioural manipulation overriding host-acceptance cues. These findings demonstrate that this cultivar combination does not provide consistent associational resistance and can create associational susceptibility for non-viruliferous <i>R. padi</i>, indicating that species-specific vector ecology and virus-mediated behavioural modification must be integrated into the design of cultivar diversification strategies for BYDV management in cereals.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimising the greenhouse cultivation of Lotus japonicus under dimmable LED","authors":"Liliya Yaneva-Roder,&nbsp;Caroline Gutjahr,&nbsp;Karin I. Köhl","doi":"10.1111/aab.70151","DOIUrl":"https://doi.org/10.1111/aab.70151","url":null,"abstract":"<p>Legumes are important crops as a source of plant-based proteins, and their independence of nitrogen fertilizers reduces the carbon footprint of cropping systems. Within the family, <i>Lotus japonicus</i> is an important model system for research on arbuscular mycorrhiza and root nodule symbiosis. Genetic approaches require year-round reproducible growth and seed production. Thus, we aimed to optimise the cultivation system of <i>L. japonicus</i> in a modern research greenhouse with dimmable LEDs while reducing energy consumption. Dimmable LEDs allow us to regulate the light provided by energy-consuming lamps in response to the ambient light intensities to achieve constant daily light integrals (DLIs) for the plants inside the greenhouse. The use of moving lamps can further reduce the number of LEDs per area, provided the cultivated species tolerates light fluctuations. In our study, we compared the plant development and the seed production during spring/summer and autumn/winter in two ecotypes (Gifu B-129, MG-20). Plants were cultivated under stationary or moving LEDs with two fertilisation schemes and two seed pre-treatments. Heat-treatment of the seed had no negative effect on the germination rate and the plant development and can be recommended as a standard hygiene procedure. The weekly fertilisation of <i>L. japonicus</i> can be replaced by adding slow-release fertilizer at the start of the cultivation. Cultivation on increased micro- or macronutrient supply did not increase pod yield; we thus conclude that the plants were not nutrient limited. <i>L. japonicus</i> developed more slowly and produced lower yields in the fluctuating light of moving LEDs than under stationary LEDs. The difference was enhanced during times of low ambient light intensities. As previously observed, Gifu developed more slowly than MG-20. While the reduced initial growth rate in the first weeks may be caused by the lower seed weight of Gifu, the delay in subsequent development seemed to result from a higher sensitivity of Gifu to low light intensities and light intensity fluctuations, as the difference between the ecotypes decreased with increasing DLI. In conclusion, we developed an optimised cultivation system for <i>L. japonicus</i> in a climate-controlled greenhouse by dimming LED in response to ambient light intensities to achieve a DLI of 20 to 25 mol m⁻² d⁻¹.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing G × E interactions to improve durum wheat resilience: Evidence from irrigated and drought-stressed trials","authors":"Reza Mohammadi,&nbsp;Mohammad Armion,&nbsp;Moslem Abdipour,&nbsp;Mahnaz Rahmati,&nbsp;Kamal Shahbazi,&nbsp;Hossein Hadavand Mirzaei","doi":"10.1111/aab.70116","DOIUrl":"https://doi.org/10.1111/aab.70116","url":null,"abstract":"<p>Durum wheat (<i>Triticum durum</i> Desf.) is a critical staple and cash crop in semi-arid regions, yet its productivity remains highly vulnerable to climate-induced abiotic stresses, particularly drought and rising temperatures. This study leverages data from the 30th Elite Regional Durum Wheat Yield Trials conducted across 13 rainfed and irrigated environments in Iran (2022–2025) to dissect genotype × environment (G × E) interactions and identify high-performing, stable genotypes for deployment under increasing climatic uncertainty. Using an integrative analytical framework—combining Additive Main Effects and Multiplicative Interaction (AMMI), Genotype plus genotype-by-environment (GGE) biplot, and Partial Least Squares (PLS) regression with 19 climatic covariates—we quantified the relative contributions of environment, genotype, and their interaction, delineated environmental groups, and identified key climatic drivers of yield variation. Results revealed that environment accounted for 81% of total yield variance, with terminal drought (low June rainfall) imposing a universal constraint. G × E interaction, though modest in magnitude (7.65%), was over six times larger than genotype main effects, underscoring its operational relevance for varietal recommendation. Breeding lines G12 (CIMMYT-derived) and G24 (Iranian) emerged as top candidates: G12 featured among the top-four performers in 10 of 13 environments and ranked first in 7, while G24 displayed exceptional stability and broad adaptability. PLS modelling identified February and April rainfall—coinciding with tillering to heading—as the strongest climatic predictors of yield, surpassing total seasonal precipitation in explanatory power; late-season (May–June) temperature also significantly modulated G × E, particularly under terminal heat stress. Environment evaluation highlighted KD3 (Khorramabad) and KH3 (Kermanshah) as the most discriminative and representative test sites, whereas MN4 (Moghan) and IM5 (Ilam) served as effective stress filters. Collectively, our findings support an empirical framework that integrates pattern recognition (AMMI/GGE) with environmental modelling (PLS) to guide environment-targeted selection—providing a foundation for developing durum wheat varieties with improved adaptation to Iran's heterogeneous rainfed agroecologies.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selecting Trichoderma and Pseudomonas species from pigeonpea rhizosphere microbial complex and profiling potential candidates for management of Fusarium wilt","authors":"Keshav Chandra Bagale,&nbsp;Saroj Belbase,&nbsp;Md. Mahtab Rashid,&nbsp;Gagan Kumar,&nbsp;P. R. Ashwinkumar,&nbsp;Sudheer Kumar Yadav,&nbsp;Anirudha Chattopadhyay,&nbsp;Raina Bajpai,&nbsp;Dawa Dolma Bhutia,&nbsp;Mariya Ansari,&nbsp;Aalok Mishra,&nbsp;Vipin Verma,&nbsp;Sonu Kumari,&nbsp;Ankita Sarkar","doi":"10.1111/aab.70149","DOIUrl":"https://doi.org/10.1111/aab.70149","url":null,"abstract":"<p>Pigeonpea wilt, caused by the soil-inhabiting <i>Fusarium udum</i>, is a severe threat to crop production, attributed to its aetiology and sustainable management. Host plant resistance is the only deployable practice under agricultural sustainability whose durability can be achieved through the application of native rhizospheric microbes, as currently practised. Thus, it is necessary to explore the native rhizospheric microbiome of pigeonpea plants grown in sick plots to isolate and assess candidate microbes. In the present investigation, four isolates from the 28 <i>Pseudomonas</i> spp. and <i>Trichoderma</i> spp. were selected, namely <i>Pseudomonas</i> spp. P-9 and P-28, and <i>Trichoderma</i> spp. K-1 and K-2, based on their biochemical and antagonistic characterization. All four isolates exhibited significant plant growth-promoting abilities and showed higher antagonistic potential against the wilt phytopathogen. Phylogenetic studies revealed the selected <i>Pseudomonas</i> spp. isolates closer to <i>Pseudomonas aeruginosa, Pseudomonas fluorescens</i>, and <i>Pseudomonas azotoformans</i>. Similarly, the selected <i>Trichoderma</i> spp. isolates were identified as <i>Trichoderma atroviride</i>, marking the first report of its isolation from the pigeonpea rhizosphere. The selected isolates also enhanced plant defence responses to the phytopathogen by upregulating PAL, PO, CAT, and lignification, indicating multifaceted traits. Thus, the isolates can be considered potential candidates for biological control and the development of synthetic microbial communities.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly essential oil nanoformulations against root-knot nematodes","authors":"Hamdy R. Soltan,&nbsp;Mohamed A. Radwan,&nbsp;Gomaa M. Gomaa,&nbsp;Mohamed H. Khalifa","doi":"10.1111/aab.70147","DOIUrl":"https://doi.org/10.1111/aab.70147","url":null,"abstract":"<p>The root-knot nematode (<i>Meloidogyne incognita</i>) poses a serious threat to global agriculture, necessitating an ongoing effort to discover new alternatives to conventional nematicides. The use of nanoformulations for sustainable pest control is rapidly evolving. Nevertheless, the effectiveness of garlic essential oil-based nanoemulsion (GEO-NE) for nematode control remains limited. Therefore, this study focused on analysing the chemical composition of garlic essential oil (GEO), and developing three distinct GEO-NEs. These formulations were subsequently characterized and evaluated for their in vivo nematicidal properties against <i>M. incognita</i> attacking tomatoes. GC–MS analysis revealed diallyl disulphide (66.68%), diallyl sulphide (8.56%), and diallyl trisulphide (7.4%) as the major constituents in GEO. The GEO was utilized to develop nanoemulsions using a high-energy emulsification technique, resulting in a uniform and stable dispersion. This was accomplished by adding Tween 20 (F1), Tween 80 (F2), or a blend of Tween 80/Span 80 (F3) as surfactants, keeping varied emulsifier-to-garlic oil ratios. The three GEO-NEs demonstrated particle sizes (≤130 nm), a polydispersity index (≤0.42), and zeta potential values between −12.7 and 4.50 mV. Excellent stability and spherical morphology were also achieved under diverse stress conditions. Furthermore, the findings indicated that all GEO-NEs effectively manage <i>M. incognita</i> by decreasing root galling and 2nd stage juvenile's numbers in the soil, while enhancing tomato growth. Specifically, F1 and F2 demonstrated greater nematicidal efficacy compared to that of the tested nematicides: oxamyl and cadusafos. Our findings underscore the GEO-NE efficacy as a promising bio-rational option for greenhouse root-knot nematode management, strengthening a more sustainable integrated nematode management approach.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional quality traits in pea seed: Current knowledge and opportunities for genetic improvement","authors":"Ahmed O. Warsame,&nbsp;Janneke Balk","doi":"10.1111/aab.70145","DOIUrl":"https://doi.org/10.1111/aab.70145","url":null,"abstract":"<p>Pea is a major pulse crop valued for its high protein, carbohydrate, and mineral content, as well as its agronomic role in sustainable cropping systems. In recent years, pea and other pulses are increasingly recognised as key components of the shift towards climate-neutral, healthier plant-based diets. The use of pea-derived ingredients is expected to expand across a wide range of end uses, including meat alternatives and bioplastics. Consequently, research efforts are ongoing to better understand the genetic and biochemical basis of the nutritional and functional traits of pea seed to develop cultivars that meet existing and new market demands. This review synthesises current knowledge on phenotypic diversity, genetic control and breeding potential of key nutritional and sensory attributes of mature pea seeds, with additional scoping data for two traits, sugars and the iron-storage protein ferritin. We discuss seed protein concentration and composition, starch content, resistant starch, raffinose family oligosaccharides, dietary fibre, mineral density, phytate, as well as colour and flavour components such as anthocyanins and saponins. This review highlights opportunities, challenges, and knowledge gaps in translating recent advances in pea genomics into the development of pea cultivars with enhanced nutritional value and consumer acceptability, contributing to global food and nutrition security.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148616246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen Climate Smart: Cutting UK agricultural emissions using a farmer-focused theory of change","authors":"Emily J. Guest,&nbsp;Roger Vickers,&nbsp;Tom Allen-Stevens,&nbsp;John McArthur,&nbsp;Daniel Kindred,&nbsp;Pietro P. M. Iannetta,&nbsp;Becky Howard,&nbsp;Tom Wilkinson,&nbsp;Ben Hague,&nbsp;Skye Melita,&nbsp;Jacqueline Hannam","doi":"10.1111/aab.70146","DOIUrl":"https://doi.org/10.1111/aab.70146","url":null,"abstract":"<p>The UK is facing increasing pressure to achieve net zero emissions to limit environmental damage and climate change. The agricultural sector contributes 12% of UK anthropogenic greenhouse gas (GHG) emissions and thus has a crucial role in reducing UK total emissions. Despite knowledge and solutions being generated by agricultural research projects, many struggle to make impactful and measurable change which lasts beyond the lifetime of a short funding cycle. In this paper, we use the lack of uptake of UK-grown pulses, especially faba beans, despite their large potential for cutting carbon emissions, as an example of the gap between research and land management change. Despite their well-known environmental and nutritional benefits, pulses only comprise an estimated 5% (1 in 20) of UK arable rotations, compared to the rotational maximum of 20% (1 in 5), a switch which would reduce GHG emissions by 3.4 Mt CO₂ equivalent (<i>e</i>) per annum. This would be equivalent to 7% of UK agricultural emissions, mainly achieved through reducing mineral nitrogen fertiliser usage and replacing soybean meal in livestock rations. While it is recognised that this 3.4 Mt per year target reduction cannot be achieved in the duration of a 4-year research programme, we propose a “theory of change” for maximising lasting impact that can be followed when designing future agricultural research programmes. Using the Nitrogen Climate Smart (NCS) project as a case study, we demonstrate a pathway to impact through meaningful farmer engagement, co-designed research questions, on-farm trials and knowledge exchange, essential to delivering long-term and long-lasting shifts in land management practice and climate impact, alongside bridging the gap between research, practice and policy.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.70146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148615469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of phosphite-based defence induction and restoration of soil and root microbial activity on mitigating huanglongbing in sweet orange (Citrus sinensis L.)","authors":"Mohammad Saeed Tadayon,&nbsp;Jahanshah Saleh,&nbsp;Ali Asadi Kangarshahi,&nbsp;Sohrab Sadeghi","doi":"10.1111/aab.70144","DOIUrl":"https://doi.org/10.1111/aab.70144","url":null,"abstract":"<p>Huanglongbing (HLB), caused by <i>Candidatus</i> Liberibacter asiaticus (CLas), critically limits citrus production by disrupting root function, nutrient acquisition, phloem transport, and canopy physiology. These constraints are more pronounced in semi-arid orchards where soil degradation intensifies disease severity and restricts recovery. This 4-year (2021–2024) multi-orchard field experiment evaluated an integrated management strategy combining enhanced nutrition (ENP), biochar-driven root bioactivation (BIO), and phosphite-mediated defence induction (DEF) to restore soil health and improve physiological resilience of HLB-affected sweet orange trees. Treatments (Control (CON), ENP, ENP + BIO, and ENP + BIO + DEF) were applied in a randomized block design. Soil biochemical attributes, microbial biomass (PLFA), enzymatic activity, and arbuscular mycorrhizal colonization were quantified, alongside assessments of root architecture, leaf nutrient status, chlorophyll content, gas exchange, CLas titres (qPCR), disease severity, and yield. The integrated ENP + BIO + DEF treatment was best for improving soil organic carbon, cation exchange capacity, nutrient availability, soil microbial biomass and activity, and mycorrhizal colonization, while reducing bulk density. These improvements facilitated strong recovery of fine-root traits, including greater root length, surface area, and vitality. Enhanced root function elevated leaf nutrient concentrations, photosynthetic efficiency, and chlorophyll content. CLas titres decreased by up to 96% relative to controls, with corresponding reductions in disease severity. Yield increased by 119%, accompanied by improved fruit quality indices. Economic analysis confirmed that the integrated strategy provided the highest profitability despite greater input costs. This biologically grounded framework demonstrates that simultaneous restoration of soil function, root system rehabilitation, and activation of systemic defences is essential for sustainable mitigation of HLB in degraded, nutrient-limited citrus orchards.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148466737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-trait selection of common bean lines resistant to Meloidogyne incognita","authors":"Líllian Beatriz Januario Bibiano,&nbsp;Bruna Marques Moreno,&nbsp;Luis Eduardo Aranha Camargo,&nbsp;Alisson Fernando Chiorato,&nbsp;Saulo Chaves,&nbsp;Maria Lucia Carneiro Vieira","doi":"10.1111/aab.70143","DOIUrl":"https://doi.org/10.1111/aab.70143","url":null,"abstract":"<p><i>Meloidogyne incognita</i> (root-knot nematode; RKN) is one of the most damaging soilborne pathogens affecting the common bean (<i>Phaseolus vulgaris</i> L.). Control relies primarily on resistant cultivars, making accurate resistance phenotyping a key component of breeding programmes. Here, we developed an integrated phenotyping approach to identify resistant genotypes in a recombinant inbred line (RIL) population. For initial screening, 361 RILs were evaluated with three replications for galling index (GI), number of galls (NG), and egg masses (EM) at 60 days after inoculation (DAI). A subset of 24 segregating RILs was further assessed in a second trial for GI, NG, EM, and reproduction factor (RF), with seven replications at 30 and 60 DAI. A multi-trait factor analytic mixed model was used to derive an overall resistance index (ORI) for genotype classification into moderately resistant (MR), intermediate (I), and susceptible (S) classes. We also assessed the potential of a qPCR-based phenotyping protocol using two contrasting RILs from the segregating subset. High heritability (&gt;0.8) and strong genotypic correlations among resistance components were observed in the RIL segregants, indicating a robust genetic basis for selection. MR genotypes consistently exhibited reduced GI, NG, EM, and RF, and transgressive segregants were identified within the MR group, confirming that the ORI framework effectively distinguished resistance levels. Moreover, later evaluation improved genotype classification and revealed resistance shifts. The rank correlation based on the ORI between 30 and 60 DAI was 0.88. qPCR-based phenotyping consistently discriminated MR and S lines in agreement with classical phenotyping, supporting its use as a complementary evaluation tool. Overall, our results validate an integrative multi-trait strategy for more precise resistance phenotyping and genotype selection.</p>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"189 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148343122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}