Annals of Applied Biology最新文献_第3页

Engineering biology and the positive regulatory pathway in Brazil 工程生物学和巴西的积极调控途径

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-02-18 DOI: 10.1111/aab.12978

Marcelo Freire, Katarzyna Adamala, Alexandre Nepomuceno, Paul Christou, Elibio Rech

引用次数: 0

Non-Streptomyces endophytic actinomycetes from Bromus auleticus as a potential biocontrol tool for biotic stress alleviation and plant growth promotion 非链霉菌内生放线菌作为缓解生物胁迫和促进植物生长的潜在生物防治工具

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-02-16 DOI: 10.1111/aab.12973

Ivana Florencia Della Mónica, Giselle Querejeta, Leopoldo J. Iannone, M. Victoria Novas, J. Martin Scervino

{"title":"Non-Streptomyces endophytic actinomycetes from Bromus auleticus as a potential biocontrol tool for biotic stress alleviation and plant growth promotion","authors":"Ivana Florencia Della Mónica, Giselle Querejeta, Leopoldo J. Iannone, M. Victoria Novas, J. Martin Scervino","doi":"10.1111/aab.12973","DOIUrl":"https://doi.org/10.1111/aab.12973","url":null,"abstract":"Plant pathogens can cause significant losses in plant production. To address this issue, it is crucial to develop innovative technologies that promote sustainability in production. One potential solution is the use of plant growth-promoting microorganisms, particularly those with biocontrol properties. In this study, root-endophytic actinomycetes isolated from Bromus auleticus, a South American forage grass, were investigated. Their ability to promote plant growth and their biocontrol activity against fungal phytopathogens were analysed. The actinomycetes were characterised molecularly, morphologically, and physiologically. Their ability to solubilise P, fixate N2, produce phytohormones, nitrate, siderophores, phenazines and lytic enzymes was assessed. Both in vitro dual-antagonistic and in vivo biocontrol assays were performed. The strains were identified as species of the Micromonospora and Microbispora genus. They were able to produce auxins, solubilise phosphorus and release lytic enzymes, and nitrate. In vitro, both species of Micromonospora aff. halotolerans reduced the growth of fungal phytopathogens by 12%–34%. In particular, the strain Micromonospora aff. halotolerans TWB21 was found to reduce the pre-emergence effects of Fusarium oxysporum growth when the actinomycete was inoculated by seed immersion. Moreover, this strain improved the seed emergence, plant establishment and plant growth parameters under biotic stress. These results remark the importance of endophytic actinomycetes with biocontrol and plant growth-promoting abilities in enhancing their host establishment and biomass production. This reinforces the idea that Micromonospora strains could be applied as biocontrol agents on grasses with agronomic interest.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"349-363"},"PeriodicalIF":2.2,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapeseed (Brassica napus L.) response to salinity and seed priming with NaCl 油菜（Brassica napus L.）对盐胁迫和NaCl胁迫的响应

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-02-03 DOI: 10.1111/aab.12974

Christos A. Damalas, Spyridon D. Koutroubas

{"title":"Rapeseed (Brassica napus L.) response to salinity and seed priming with NaCl","authors":"Christos A. Damalas, Spyridon D. Koutroubas","doi":"10.1111/aab.12974","DOIUrl":"https://doi.org/10.1111/aab.12974","url":null,"abstract":"Laboratory and pot trials were conducted to study germination performance of rapeseed (Brassica napus L., genotype Excalibur) under NaCl salinity after seed priming with 20 mM NaCl for 24 h. In the laboratory trial, salinity levels of 80, 160, and 240 mM NaCl reduced the germination percentage by 14, 56, and 80 percentage points, respectively compared to the non-saline control. NaCl priming lowered the detrimental effect of salt stress on germination by promoting germination from 81% to 85% under salinity of 80 mM, from 39% to 68% under salinity of 160 mM, and from 15% to 56% under salinity of 240 mM NaCl (maximum alleviation). Furthermore, NaCl priming increased the germination tolerance index and the vigour index of seedlings as compared with the control seeds. Relative water content of the leaves was decreased with increasing salinity, showing limited water availability and a loss of turgor in the leaves. NaCl priming reduced the decrease in relative water content under salinity. Concerning the pot trial, the salinity level of 240 mM NaCl reduced the dry weight of rapeseed seedlings by 80.9% in the non-primed seeds and by 55.8% in the primed seeds. NaCl priming improved the mean productivity index by 1.26 times under salinity of 240 mM NaCl and the harmonic mean index by 1.98 times under salinity of 240 mM NaCl compared with non-primed seeds. Salinity limited seed performance of rapeseed, whereas NaCl priming improved seed performance and early growth in saline environment. The priming method needs to be examined in a selection of relevant genotypes under a range of saline conditions.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"187 1","pages":"16-23"},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of sugars on the gustatory response, longevity and realized fecundity of the egg parasitoid Trissolcus basalis 糖对卵寄生蜂基底三棱的味觉反应、寿命和实现繁殖力的影响

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-01-25 DOI: 10.1111/aab.12979

Margot W. J. Geerinck, Isabelle Stockmans, Felix Wäckers, Antonino Cusumano, Hans Jacquemyn, Bart Lievens

{"title":"Effects of sugars on the gustatory response, longevity and realized fecundity of the egg parasitoid Trissolcus basalis","authors":"Margot W. J. Geerinck, Isabelle Stockmans, Felix Wäckers, Antonino Cusumano, Hans Jacquemyn, Bart Lievens","doi":"10.1111/aab.12979","DOIUrl":"https://doi.org/10.1111/aab.12979","url":null,"abstract":"Parasitoids are widely used as biological control agents to manage insect pest populations in greenhouses. To meet their energetic and nutritional requirements, adult parasitoids consume carbohydrate-rich sources such as (extra-)floral nectar and honeydew. However, these sugar sources are often scarce or even absent in greenhouses, compromising the effectiveness of parasitoids as biological control agents. To remedy this, artificial sugar solutions can be provided to sustain parasitoid populations. To develop an artificial sugar solution that supports parasitoids, proper selection of sugars is critical. In this study, we investigated the innate gustatory response and survival of the egg parasitoid Trissolcus basalis (Wollaston) on eight plant- and/or insect-derived sugars (fructose, glucose, maltose, mannose, melezitose, rhamnose, sucrose and trehalose). Our results show that T. basalis can consume a wide range of sugars and survive on them. Five sugars (sucrose, fructose, maltose, melezitose and trehalose) increased its longevity more than seven-fold compared to the water control. For two sugars, sucrose and trehalose, the parasitoid's realized fecundity was monitored after various time periods during which the parasitoids were fed with the sugars (1 day, 1 week, 2 weeks, and 3 weeks). The results indicate a prolonged period of parasitism compared to the water control because of a longer lifespan when fed on the sugars. Altogether, our findings provide valuable insights for the development of an artificial sugar solution that supports the performance of T. basalis, potentially enhancing the biological control of important pest species such as Nezara viridula (Linnaeus).","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"187 1","pages":"24-33"},"PeriodicalIF":2.2,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic analysis of dinotefuran resistance in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), using the experimental evolution approach 褐飞虱（半翅目：褐飞虱科）对氟虫胺抗性的表型分析

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-01-22 DOI: 10.1111/aab.12957

Tomohisa Fujii, Masaya Matsumura, Sachiyo Sanada-Morimura

{"title":"Phenotypic analysis of dinotefuran resistance in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), using the experimental evolution approach","authors":"Tomohisa Fujii, Masaya Matsumura, Sachiyo Sanada-Morimura","doi":"10.1111/aab.12957","DOIUrl":"https://doi.org/10.1111/aab.12957","url":null,"abstract":"Insecticide applications for controlling insect pests drive the rapid phenotypic evolution of resistance traits within populations. In two decades, the brown planthopper, Nilaparvata lugens, has rapidly developed resistance to imidacloprid, a neonicotinoid insecticide, but its resistance to dinotefuran has not developed as quickly. We assume that the genetic mechanism underlying the resistance evolution of N. lugens differs between the imidacloprid- and dinotefuran-resistant phenotypes. Dinotefuran-resistant strains, collected from a paddy field in Kumamoto in 2013 (Res-D13) and 2014 (Res-D14), were subjected to quantitative genetic analysis. The realized heritabilities of dinotefuran resistance were 0.091 (Res-D13) and 0.084 (Res-D14) after the 15th generation of selection. Reciprocal cross experiments between Res-D13 and the susceptible strain (Sus-strain), and between Res-D14 and the Sus-strain, showed that the degree of dominance for dinotefuran resistance ranged from 0.34 to 0.57. Analysis of the F2 population and backcrosses to the parental strains indicated that dinotefuran resistance is a quantitative trait controlled by multiple genes on autosomal chromosomes. We propose that the realized heritability and the mode of inheritance of dinotefuran resistance well explain the slow development of dinotefuran resistance in field N. lugens populations.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 2","pages":"195-203"},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematicidal activity of Streptomyces aquilus JXGZ01 against the root-knot nematode Meloidogyne incognita aquilus链霉菌JXGZ01对根结线虫的杀线虫活性

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-01-22 DOI: 10.1111/aab.12967

Xueliang Xu, Linjuan Fan, Caiyun Wu, Zirong Liu, Jian Yao, Yingjuan Yao

{"title":"Nematicidal activity of Streptomyces aquilus JXGZ01 against the root-knot nematode Meloidogyne incognita","authors":"Xueliang Xu, Linjuan Fan, Caiyun Wu, Zirong Liu, Jian Yao, Yingjuan Yao","doi":"10.1111/aab.12967","DOIUrl":"https://doi.org/10.1111/aab.12967","url":null,"abstract":"In this study, we evaluated the biocontrol effect against Meloidogyne incognita of Streptomyces (strain JXGZ01) obtained from yam field soil. Results showed that the mortality of second-stage juveniles (J2s) treated with an undiluted culture filtrate of JXGZ01 for 12 and 24 h was 70.86% and 85.58%, respectively. The twofold dilution resulted in 66.41% and 81.55% mortality for 12 and 24 h, respectively. Both strong acids and alkali significantly reduced the nematicidal activity of the culture filtrate, but neither temperature nor ultraviolet irradiation had any significant effect. In addition, two, four and eightfold dilutions inhibited the hatching of M. incognita eggs by 91.3%, 83.7% and 75.7%, respectively. Finally, the results of a pot experiment showed that the nematodes treated with different dilutions of culture filtrate significantly reduced the number of galls by 65.87%–97.30%. Compared with those of the control group, the treatments reduced the number of egg masses and J2s by 38.17%–92.84% and 37.57%–93.82%, respectively, and the eggs hatching rate was reduced by 27.92%–45.27%, relative to the control group. Data indicate that strain JXGZ01 has a potential and application value for development as a biocontrol agent for M. incognita.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"302-310"},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the impact of host plant factors on the oviposition behaviour of the cabbage stem flea beetle (Psylliodes chrysocephala) 寄主植物因子对白菜茎蚤甲虫产卵行为影响的研究

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-01-17 DOI: 10.1111/aab.12976

Thomas Giguère, Valentin Bailly, Thomas Rey, Anne Marie Cortesero, Maxime R. Hervé

{"title":"Understanding the impact of host plant factors on the oviposition behaviour of the cabbage stem flea beetle (Psylliodes chrysocephala)","authors":"Thomas Giguère, Valentin Bailly, Thomas Rey, Anne Marie Cortesero, Maxime R. Hervé","doi":"10.1111/aab.12976","DOIUrl":"https://doi.org/10.1111/aab.12976","url":null,"abstract":"For many phytophagous insects, oviposition plays a key role in the selection of an appropriate host. A more comprehensive understanding of the factors influencing this behaviour in pest insects provides a basis for the development of new management strategies. Herein, the determinants of oviposition were assessed in the cabbage stem flea beetle, Psylliodes chrysocephala (Coleoptera: Chrysomelidae), an insect specialized on the Brassicaceae family and a major pest of oilseed rape crops in Europe. To this end, a controlled condition experiment was conducted, in which a range of host plants exhibiting varying degrees of acceptability for feeding were presented to mature females. A superior host, Brassica rapa pekinensis, an intermediate one, Brassica napus, and a non-host plant, Iberis amara, were used. An additional treatment involved wrapping the plants in micro-perforated plastic bags to prevent feeding on aerial parts. The results demonstrated that contact with the host plant, and more particularly feeding, stimulated oviposition, with this effect being more pronounced in B. napus. Indeed, for the same amount of feeding, females laid significantly more eggs when feeding on B. napus than when feeding on B. rapa pekinensis. Additionally, a sequential experiment demonstrated that the intensity of oviposition is subject to rapid adaptive changes, as it is exclusively dependent on the current dietary conditions, with no influence of past dietary regimens. The quantification of macronutrients indicated the potential influence of plant-digestible carbohydrates on these outcomes. Further investigation is required to determine the impact of the plant nutritional quality and defences on oviposition.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"187 1","pages":"34-44"},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and virulence traits of Pseudomonas syringae pv. syringae isolated from various hosts in Serbia 丁香假单胞菌的遗传多样性及毒力性状。从塞尔维亚不同宿主分离的丁香属

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-01-15 DOI: 10.1111/aab.12972

Tatjana Popović Milovanović, Andrea Kosovac, Aleksandra Jelušić, Marco Scortichini, Nenad Trkulja, Slaviša Stanković, Renata Iličić

{"title":"Genetic diversity and virulence traits of Pseudomonas syringae pv. syringae isolated from various hosts in Serbia","authors":"Tatjana Popović Milovanović, Andrea Kosovac, Aleksandra Jelušić, Marco Scortichini, Nenad Trkulja, Slaviša Stanković, Renata Iličić","doi":"10.1111/aab.12972","DOIUrl":"https://doi.org/10.1111/aab.12972","url":null,"abstract":"In this study, we assessed the genetic relatedness of 11 representative Pseudomonas syringae pv. syringae (Pss) strains isolated in Serbia from different herbaceous and woody plant species, as well as one reference Pss strain, using various molecular tools such as repetitive element palindromic PCR, arbitrarily primed PCR, insertion sequences 50 PCR (IS50-PCR) and multilocus sequence analysis (MLSA). Their virulence traits were also assessed by inoculating detached pear and lilac leaves, as well as immature cherry fruitlets. The results indicated genetic heterogeneity among the strains, which formed a complex network with prominent clustering patterns. While four distinct groups could be recognised when comparative Pss strains from the database were included in the analyses, the strains in focus of this study segregated into two distinct genetic groups labelled PssG-2 and PssG-3. MLSA findings mostly matched the IS50-PCR results, while virulence assays helped distinguish sweet cherry (RE05, RE3) and pumpkin (PS-T71) strains as the most virulent. Only strain Pss25, originating from pear, exhibited host specificity.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"334-348"},"PeriodicalIF":2.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium spp. associated with asparagus crowns and the influence of the preplant fumigant metam-sodium in Michigan crown nurseries 与芦笋冠相关的镰刀菌及其株前熏蒸剂元钠的影响

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2025-01-09 DOI: 10.1111/aab.12971

Maria Camila Buitrago-Acosta, Douglas H. Minier, Sara Getson, Carmen M. Medina-Mora, Mary K. Hausbeck

{"title":"Fusarium spp. associated with asparagus crowns and the influence of the preplant fumigant metam-sodium in Michigan crown nurseries","authors":"Maria Camila Buitrago-Acosta, Douglas H. Minier, Sara Getson, Carmen M. Medina-Mora, Mary K. Hausbeck","doi":"10.1111/aab.12971","DOIUrl":"https://doi.org/10.1111/aab.12971","url":null,"abstract":"Fusarium crown and root rot (FCRR) of asparagus caused by Fusarium spp., results in plant stunting, wilting, vascular discoloration, and root rot reducing the longevity and productivity of this perennial vegetable. Metam-sodium is a broad-spectrum pre-plant fumigant commonly used in Michigan asparagus nurseries to mitigate FCRR by reducing disease presence in 1-year-old crowns that are used for establishing production fields. The objective of the current study was to compare the diversity of Fusarium species associated with nursery-grown asparagus crowns from soils fumigated preplant with metam-sodium to those from unfumigated nurseries. Based on sequences of the TEF-1α (transcription elongation factor 1-alpha) region, seven Fusarium spp. were identified including F. oxysporum, F. proliferatum, F. solani, F. acuminatum, F. avenaceum, F. graminearum and F. incarnatum-equiseti. Fusarium oxysporum was the most frequently isolated species (92%). No significant differences were noted between Fusarium populations isolated from nurseries that had been fumigated with metam-sodium and those isolated from the nursery that had not been fumigated. Pathogenic isolates of F. oxysporum were recovered from nurseries at a similar rate regardless of fumigation status. These findings indicate that metam-sodium is inadequate as a pre-plant treatment for Fusarium crown and root rot and alternative strategies need to be explored.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"323-333"},"PeriodicalIF":2.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12971","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beauveria pseudobassiana: A good candidate for controlling Diprion pini L. (Hymenoptera: Diprionidae) 伪球孢白僵菌：一种防治双翅虫的候选菌（膜翅目：双翅虫科）

IF 2.2 3区农林科学

Annals of Applied Biology Pub Date : 2024-12-30 DOI: 10.1111/aab.12970

Ali Sevim, Elif Sevim

{"title":"Beauveria pseudobassiana: A good candidate for controlling Diprion pini L. (Hymenoptera: Diprionidae)","authors":"Ali Sevim, Elif Sevim","doi":"10.1111/aab.12970","DOIUrl":"https://doi.org/10.1111/aab.12970","url":null,"abstract":"The common pine sawfly, Diprion pini (Linnaeus, 1758) (Hymenoptera: Diprionidae), is a well-known defoliating pest of various pine forests almost all over the world, including Europe. It can cause damage to many pine species but usually opts for Pinus sylvestris Linnaeus and P. nigra subsp. laricio (Poiret) Maire. The prohibition of the use of chemical insecticides in forests (at least for Türkiye) has led to the fact that other control methods have come to the fore in the control of this pest. In this respect, biological control agents, which are eco-friendly and can persist in the field over time, providing long-term control for plant protection, have an important potential in the control of D. pini. Therefore, in this study, entomopathogenic fungi were isolated from pine forest soils and identified by gene sequencing and phylogenetic analysis. Ten isolates (DP-37, DP-38, DP-45, DP-46, DP-49, DP-53, DP-54, DP-57, DP-58 and DP-63) were identified as Beauveria pseudobassiana, four isolates (DP-35, DP-41, DP-52, and DP-61) were identified as B. bassiana, and only one isolate was identified as Metarhizium robertsii (DP-15). All isolates were tested against the larvae of the pest under laboratory conditions, and the highest mortality and mycosis values (96.6% and 63.3%, respectively) were obtained from B. pseudobassiana DP-57. This isolate was also tested against the pest under outdoor conditions using different conidial concentrations. Based on probit analysis, the LC50 and LC90 values were estimated to be 1.309 × 107 and 1.21 × 1010 conidia/ml, respectively. The results showed that B. pseudobassiana DP-57 could be a good candidate in the biological control of D. pini.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"186 3","pages":"311-322"},"PeriodicalIF":2.2,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143884190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0