Genetic diversity and virulence traits of Pseudomonas syringae pv. syringae isolated from various hosts in Serbia

IF 2.2 3区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY

Annals of Applied Biology Pub Date : 2025-01-15 DOI:10.1111/aab.12972

Tatjana Popović Milovanović, Andrea Kosovac, Aleksandra Jelušić, Marco Scortichini, Nenad Trkulja, Slaviša Stanković, Renata Iličić

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Abstract

In this study, we assessed the genetic relatedness of 11 representative Pseudomonas syringae pv. syringae (Pss) strains isolated in Serbia from different herbaceous and woody plant species, as well as one reference Pss strain, using various molecular tools such as repetitive element palindromic PCR, arbitrarily primed PCR, insertion sequences 50 PCR (IS50-PCR) and multilocus sequence analysis (MLSA). Their virulence traits were also assessed by inoculating detached pear and lilac leaves, as well as immature cherry fruitlets. The results indicated genetic heterogeneity among the strains, which formed a complex network with prominent clustering patterns. While four distinct groups could be recognised when comparative Pss strains from the database were included in the analyses, the strains in focus of this study segregated into two distinct genetic groups labelled PssG-2 and PssG-3. MLSA findings mostly matched the IS50-PCR results, while virulence assays helped distinguish sweet cherry (RE05, RE3) and pumpkin (PS-T71) strains as the most virulent. Only strain Pss25, originating from pear, exhibited host specificity.

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丁香假单胞菌的遗传多样性及毒力性状。从塞尔维亚不同宿主分离的丁香属

本研究对11种具有代表性的丁香假单胞菌进行了遗传亲缘性评价。利用重复元素回文PCR、任意引物PCR、插入序列50 PCR （IS50-PCR）和多位点序列分析（MLSA）等多种分子分析工具，对塞尔维亚不同草本和木本植物中分离的丁香属（syringae, Pss）菌株以及1个参考菌株进行了分析。通过接种梨、丁香离体叶片和樱桃未成熟果实，对其毒力性状进行了评价。结果表明，各菌株之间存在遗传异质性，形成了一个复杂的网络，具有明显的聚类特征。当将数据库中的比较Pss菌株纳入分析时，可以识别出四个不同的群体，而本研究的重点菌株分为两个不同的遗传群体，分别标记为PssG-2和PssG-3。MLSA结果与IS50-PCR结果基本一致，而毒力测定有助于区分甜樱桃（RE05, RE3）和南瓜（PS-T71）菌株的毒力最强。只有梨源菌株Pss25表现出寄主特异性。

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来源期刊

Annals of Applied Biology 生物-农业综合

CiteScore

5.50

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists. The journal publishes original research papers on all aspects of applied research on crop production, crop protection and the cropping ecosystem. The journal is published both online and in six printed issues per year. Annals papers must contribute substantially to the advancement of knowledge and may, among others, encompass the scientific disciplines of: Agronomy Agrometeorology Agrienvironmental sciences Applied genomics Applied metabolomics Applied proteomics Biodiversity Biological control Climate change Crop ecology Entomology Genetic manipulation Molecular biology Mycology Nematology Pests Plant pathology Plant breeding & genetics Plant physiology Post harvest biology Soil science Statistics Virology Weed biology Annals also welcomes reviews of interest in these subject areas. Reviews should be critical surveys of the field and offer new insights. All papers are subject to peer review. Papers must usually contribute substantially to the advancement of knowledge in applied biology but short papers discussing techniques or substantiated results, and reviews of current knowledge of interest to applied biologists will be considered for publication. Papers or reviews must not be offered to any other journal for prior or simultaneous publication and normally average seven printed pages.