Insect Biochemistry and Molecular Biology最新文献

Female accessory reproductive glands of Paederus fuscipes serve as a reservoir of symbiotic pederin-producing bacteria. 雌性副生殖腺作为共生的产童精细菌的储存库。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-10-02 DOI: 10.1016/j.ibmb.2025.104408

Xuhao Song, Hui Meng, Tingbang Yang, Yujie Li, Fake Zheng, Xianghui Yan

{"title":"Female accessory reproductive glands of Paederus fuscipes serve as a reservoir of symbiotic pederin-producing bacteria.","authors":"Xuhao Song, Hui Meng, Tingbang Yang, Yujie Li, Fake Zheng, Xianghui Yan","doi":"10.1016/j.ibmb.2025.104408","DOIUrl":"https://doi.org/10.1016/j.ibmb.2025.104408","url":null,"abstract":"<p><p>Paederus fuscipes, an ecologically and medically important species, is known for its blistering toxin pederin in hemolymph. Evidence demonstrates that the toxin is synthesized by the uncultured symbiotic pederin-producing bacteria (PPB) in P. fuscipes, but the biological characteristics of PPB within the beetle host remain poorly characterized. Here, we investigated PPB abundance variations in P. fuscipes across different factors (sexes, life stages, habitats, and organs), along with their colonization sites and metabolic potentials. The findings revealed that the PPB abundance in female P. fuscipes at the level of individuals and tissues exhibited stable colonization patterns, independent of habitat and time changes. Notably, PPB dominated the bacterial community in females (relative abundance ≥ 66.08%) and nearly occupied reproductive organs (relative abundance ≥ 96.31%). Moreover, our results indicated that PPB were predominantly enriched in the accessory glands of female reproductive organs, which could serve as a reservoir for PPB proliferation. Although PPB were not cultured in this study, metagenomic binning yielded the draft genome of PPB (CheckM completeness = 85.14%, contamination = 0), in which genes related to pederin biosynthesis were identified. Phylogenetic analyses revealed that PPB formed a sister clade to Pseudomonas aeruginosa rather than nesting within the P. aeruginosa lineage. Metabolic module prediction analysis revealed specific deficiencies in PPB's energy metabolism and amino acid biosynthesis pathways, suggesting limited free-living potential for PPB. Collectively, this study provides insights into PPB biological characteristics within their beetle host and paves the way for biotechnological exploitation related to pederin production.</p>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":" ","pages":"104408"},"PeriodicalIF":3.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining the type of aerobic/anaerobic metabolism during exercise in Drosophila using incremental loading exercise. 使用增量负荷运动确定果蝇运动期间的有氧/无氧代谢类型。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-10-01 DOI: 10.1016/j.ibmb.2025.104404

Qin Yi, Meng Ding, Jinglin Liu, Xiaoya Wang, Lan Zheng

{"title":"Determining the type of aerobic/anaerobic metabolism during exercise in Drosophila using incremental loading exercise.","authors":"Qin Yi, Meng Ding, Jinglin Liu, Xiaoya Wang, Lan Zheng","doi":"10.1016/j.ibmb.2025.104404","DOIUrl":"https://doi.org/10.1016/j.ibmb.2025.104404","url":null,"abstract":"<p><p>Currently, Drosophila exercise is only classified as regular or endurance exercise, and there is no aerobic or anaerobic form of exercise, making Drosophila exercise modeling studies limited. Therefore, this study aimed to determine the type of aerobic and anaerobic exercise in Drosophila using Incremental Load exercise. A seven-day-old wild-type Drosophila was used as a study sample for exercise intervention using a Drosophila exercise device. This study was designed with six exercise intensities, including 0.31 rev/s (E1), 0.45 rev/s (E2), 0.59 rev/s (E3), 0.71 rev/s (E4), 0.83 rev/s (E5), 0.91 rev/s (E6), and Control in addition Drosophila in all exercise groups performed a one-time 2.5-h acute exercise, and the control group did no exercise intervention. Anaerobic and aerobic metabolic enzymes in Drosophila were assayed immediately after exercise. Additionally, Drosophila exercise types were determined using hemolymph lactate, respiratory quotient, trehalose concentration, and mitochondrial respiration assays. The experimental results show that at E2 exercise intensity, Drosophila mainly uses aerobic metabolism, and at E6 exercise intensity, it mainly uses anaerobic metabolism.We exercised Drosophila for two weeks at E2 and E6 exercise intensities to examine whether the physiological differences (cardiac function, climbing ability, sleep ability, lifespan, etc.) were consistent with those observed in mammals undergoing long-term aerobic and anaerobic exercise.The results showed that these differences are consistent with mammals.</p>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":" ","pages":"104404"},"PeriodicalIF":3.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of the transformer gene in sex determination and its employment in CRISPR/Cas9-based homing gene drive in the global fruit pest Drosophila suzukii 变形基因在全球水果害虫铃木果蝇性别决定中的作用及其在CRISPR/ cas9基因驱动中的应用

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-25 DOI: 10.1016/j.ibmb.2025.104406

Dan Deng , Xueying Yi , Wen Wen, Liuqing He, Wei Peng

{"title":"The role of the transformer gene in sex determination and its employment in CRISPR/Cas9-based homing gene drive in the global fruit pest Drosophila suzukii","authors":"Dan Deng , Xueying Yi , Wen Wen, Liuqing He, Wei Peng","doi":"10.1016/j.ibmb.2025.104406","DOIUrl":"10.1016/j.ibmb.2025.104406","url":null,"abstract":"<div><div>Sex determination of Diptera is established by the cascade genes such as <em>transformer</em> (<em>tra</em>), though the primary signals for sex determination differ among different insects. Here, we report the isolation, expression and function of <em>tra</em> gene in an invasive pest, <em>Drosophila suzukii</em>, and study the potential use of the <em>D. suzukii tra</em> (<em>Dstra</em>) gene in CRISPR/Cas9-based homing gene drive for genetic-based pest management. The <em>Dstra</em> gene is highly conserved in structure and has a sex-specific transcript. To test the function of this gene in sex determination, <em>Dstra</em> dsRNA was injected into embryos. Almost all XX embryos developed into masculinized phenotypic male adults with intersex morphology. Abnormal ovaries were revealed in XX pseudomales upon dissection. Based on the necessary role of <em>Dstra</em> for female development, we developed and evaluated a homing gene drive that targets <em>Dstra</em> in <em>D</em>. <em>suzukii</em>. The drive component consisting of multiplex <em>Dstra</em> single guide RNAs and Cas9 with <em>Dsvasa</em> promoter was introduced into the <em>Dstra</em> locus. Abnormal development of both the external genitalia and gonads was observed in G0 and G1 chromosomal female adults that expressed the male-specific <em>doublesex</em> (<em>dsx</em>) transcript. Interestingly, knocking out <em>Dstra</em> led to significantly reduced fertility in adults of corresponding sex and moderate transmission rates of the DsRed gene (63.54 %) were observed. Our results not only confirm the conserved function of the <em>Dstra</em> gene in sex determination, but also highlight the potential of sex conversion-based suppression gene-drive strategy targeting the <em>Dstra</em> gene in controlling of <em>D. suzukii</em> populations.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104406"},"PeriodicalIF":3.7,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

V-ATPase B mediates Bt Cry1Ac binding and toxicity in Grapholita molesta V-ATPase B介导Bt - Cry1Ac的结合和毒性作用。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-24 DOI: 10.1016/j.ibmb.2025.104407

Dandan Pan , Jiayang Feng , Jiacheng Ye, Xiaoyan Yang, Xiaoyan Zhang, Xiaofang Sha, Donghan Wang, Yanshen Fu, Chunxiao Men, Xiangqun Yuan, Yiping Li

{"title":"V-ATPase B mediates Bt Cry1Ac binding and toxicity in Grapholita molesta","authors":"Dandan Pan , Jiayang Feng , Jiacheng Ye, Xiaoyan Yang, Xiaoyan Zhang, Xiaofang Sha, Donghan Wang, Yanshen Fu, Chunxiao Men, Xiangqun Yuan, Yiping Li","doi":"10.1016/j.ibmb.2025.104407","DOIUrl":"10.1016/j.ibmb.2025.104407","url":null,"abstract":"<div><div><em>Grapholita molesta</em> is a worldwide pest. Cry1Ac is a significant alternative <em>Bacillus thuringiensis</em> (Bt) protein that exhibits substantial toxicity towards Lepidoptera insects. This investigation employed ligand blot, SDS-PAGE and liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques to identify the proteins potentially binding Cry1Ac on the peritrophic membrane (PM) of <em>G. molesta</em>. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed the highest expression of <em>GmolV-ATPase B</em> in the midgut of 4th instar larvae. Following the ingestion of Cry1Ac protoxin by <em>G. molesta</em> larvae, a notable reduction in the expression level of <em>GmolV-ATPase B</em> was observed. The interaction between GmolV-ATPase B and activated Cry1Ac toxin was confirmed through ligand blot and homologous and heterologous competition experiments. Overexpression of GmolV-ATPase B in Sf9 cells led to an increase in Cry1Ac cytotoxicity, while RNAi targeting <em>GmolV-ATPase B</em> resulted in reduced mortality. In vivo bioassays demonstrated that the combined action of GmolV-ATPase B protein and Cry1Ac protoxin significantly enhanced the toxicity of Cry1Ac towards <em>G. molesta</em> larvae compared to Cry1Ac alone. These findings shed light on the binding of Cry1Ac to PM of <em>G. molesta</em> and its insecticidal mechanism, offering a valuable important reference for the development of biopesticides targeting midgut PM proteins.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104407"},"PeriodicalIF":3.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient hyPBase-mediated transgenesis facilitates gain-of-function analysis in the cricket Gryllus bimaculatus 高效的hypbase介导的转基因促进了蟋蟀双斑鸠的功能获得分析

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-24 DOI: 10.1016/j.ibmb.2025.104405

Takahiro Ohde , Taro Nakamura , Taro Mito , Takaaki Daimon

{"title":"Highly efficient hyPBase-mediated transgenesis facilitates gain-of-function analysis in the cricket Gryllus bimaculatus","authors":"Takahiro Ohde , Taro Nakamura , Taro Mito , Takaaki Daimon","doi":"10.1016/j.ibmb.2025.104405","DOIUrl":"10.1016/j.ibmb.2025.104405","url":null,"abstract":"<div><div>Gain-of-function analysis is critical to directly assessing gene functions but remains challenging in non-traditional model insects. Recent development of the hyperactive <em>piggyBac</em> transposase (hyPBase) has improved the efficiency of germline transformation in ametabolous and holometabolous insects. Here, we report highly efficient somatic and germline transformations mediated by hyPBase in the cricket <em>Gryllus bimaculatus</em>. hyPBase-mediated misexpression of the melanin pathway gene <em>arylalkylamine N-Acetyltransferase</em> (<em>aaNAT</em>) turned the cricket body color from black to a light color, indicating a broad effect on external phenotype in the injected generation. Mosaic expression of the homeotic gene <em>Antennapedia</em> with hyPBase successfully circumvented the potential embryonic lethality, and induced antenna-to-leg homeotic transformation in a hatched nymph. Our data provide a practical methodology for gain-of-function analysis in the hemimetabolous model to understand key evolutionary innovations in insects.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104405"},"PeriodicalIF":3.7,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuticular proteins: Essential molecular code for insect survival 表皮蛋白：昆虫生存的基本分子密码

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-22 DOI: 10.1016/j.ibmb.2025.104402

Huitang Qi, Tian Liu

引用次数: 0

Sub-lethal insecticide stress alters epimutation rate but not genetic mutation rate in the pest insect Myzus persicae 亚致死杀虫剂胁迫改变了害虫桃蚜（Myzus persicae）的灭绝率，但没有改变其基因突变率。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-22 DOI: 10.1016/j.ibmb.2025.104403

Benjamin J. Hunt , Kumar Saurabh Singh , Bartlomiej J. Troczka , Joanna Mackisack , Emma Randall , Mark Mallott , Tobias Baril , James Galbraith , Bram Kuijper , Ralf Nauen , Alex Hayward , Chris Bass

{"title":"Sub-lethal insecticide stress alters epimutation rate but not genetic mutation rate in the pest insect Myzus persicae","authors":"Benjamin J. Hunt , Kumar Saurabh Singh , Bartlomiej J. Troczka , Joanna Mackisack , Emma Randall , Mark Mallott , Tobias Baril , James Galbraith , Bram Kuijper , Ralf Nauen , Alex Hayward , Chris Bass","doi":"10.1016/j.ibmb.2025.104403","DOIUrl":"10.1016/j.ibmb.2025.104403","url":null,"abstract":"<div><div>Insect pests evolve heritable resistance to insecticides through selection acting on genetic variation generated by mutation. It has been hypothesised that exposure to low doses of pesticides may increase mutation rate in pest genomes and accelerate resistance evolution. However, the impact of pesticide-induced stress on the mutation rate of insects has never been empirically tested. Here we leverage new, high-quality genomic resources for the aphid pest <em>Myzus persicae</em> in conjunction with long-term mutation accumulation experiments to interrogate spontaneous genetic and epigenetic mutation rates in insecticide-exposed and unexposed aphid lines. Our data reveal that multigenerational exposure of <em>Myzus persicae</em> to sublethal concentrations of the neonicotinoid insecticide imidacloprid does not increase genetic mutation rate. Rather we show that imidacloprid exposure results in a significantly lower epimutation rate. These findings reveal the rate of origin and composition of (epi)mutations arising in a pest insect and challenge the proposed link between pesticide exposure and the rate of mutation.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104403"},"PeriodicalIF":3.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145135902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-927 links nutrient signals and 20-hydroxyecdysone regulation and mediates oviposition in Bactrocera dorsalis miR-927在背小实蝇中连接营养信号和20-羟基脱皮激素调节并介导产卵。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-17 DOI: 10.1016/j.ibmb.2025.104401

Tong-Jun Jin , Zheng-Yang Wang , Qian-Ping Xie , Li-Yuan Zheng , Guy Smagghe , Jin-Jun Wang , Qiang Zhang , Wei Dou

{"title":"miR-927 links nutrient signals and 20-hydroxyecdysone regulation and mediates oviposition in Bactrocera dorsalis","authors":"Tong-Jun Jin , Zheng-Yang Wang , Qian-Ping Xie , Li-Yuan Zheng , Guy Smagghe , Jin-Jun Wang , Qiang Zhang , Wei Dou","doi":"10.1016/j.ibmb.2025.104401","DOIUrl":"10.1016/j.ibmb.2025.104401","url":null,"abstract":"<div><div>The interplay between lipid homeostasis and hormonal regulation is critical for reproductive success in female insects. The molecular mechanisms linking lipid metabolism to hormonal pathways, particularly ecdysteroid synthesis, remain poorly understood. In this study, we reveal a novel regulatory role of miR-927 in the oriental fruit fly, <em>Bactrocera dorsalis</em>. <em>U</em>sing <em>in vivo</em> miRNA mimic/inhibitor assays, combined with an <em>in vitro</em> dual-luciferase reporter and RNA pull-down experiments, we demonstrated that miR-927 directly targets <em>BdCYP315a1</em>, a key enzyme in ecdysteroidogenesis, thereby repressing the 20-hydroxyecdysone (20E) levels. RNA interference of <em>BdCYP315a1</em> confirmed its essential role in maintaining 20E homeostasis and proper developmental progression. Further, we show that miR-927 expression is regulated by the insulin/insulin-like signaling (IIS) pathway, linking nutritional status to hormonal control. Changes in miR-927, <em>BdCYP315a1</em>, and 20E levels corresponded with alterations in lipid content, ultimately influencing fecundity. These findings reveal a previously uncharacterized miRNA-mediated network integrating nutritional signals, hormonal regulation, and lipid metabolism to control reproductive outcomes. Our work provides novel insights into the molecular mechanisms underlying insect reproduction, highlights miRNAs as key mediators of hormone-lipid interactions, and offers valuable resources for identifying miRNA targets in non-model insect species.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104401"},"PeriodicalIF":3.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is the CncC/Keap1 complex a major factor in conferring pesticide resistance in arthropods? - A critical review CncC/Keap1复合体是节肢动物产生农药抗性的主要因素吗？-批评性评论

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-10 DOI: 10.1016/j.ibmb.2025.104400

Dries Amezian, Thomas Van Leeuwen

引用次数: 0

Single-cell transcriptomic profiling reveals diet-dependent dynamics of glucosinolate sulfatases expression and cellular origin in the midgut of Plutella xylostella 单细胞转录组学分析揭示了小菜蛾中肠硫代葡萄糖苷硫酸盐酶表达的饮食依赖动力学和细胞起源。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-09-09 DOI: 10.1016/j.ibmb.2025.104399

Hanwen Ye , Chanqin Zheng , Bing Wang , Yuqing Zhu , Chenjing Wang , Zhuobing Wang , Zhanjun Lu , Weiyi He , Wei Chen

{"title":"Single-cell transcriptomic profiling reveals diet-dependent dynamics of glucosinolate sulfatases expression and cellular origin in the midgut of Plutella xylostella","authors":"Hanwen Ye , Chanqin Zheng , Bing Wang , Yuqing Zhu , Chenjing Wang , Zhuobing Wang , Zhanjun Lu , Weiyi He , Wei Chen","doi":"10.1016/j.ibmb.2025.104399","DOIUrl":"10.1016/j.ibmb.2025.104399","url":null,"abstract":"<div><div>The diamondback moth (<em>Plutella xylostella</em>), a globally destructive pest, has Brassicaceae as its long-term co-evolved host and can also utilize Fabaceae as an alternative field host. The primary differential factor between these plant families is glucosinolates (GLs). Conventional transcriptome data revealed high midgut expression of <em>glucosinolate sulfatases</em> (<em>GSSs</em>) in response to glucosinolates. However, due to the inability of conventional transcriptomics to resolve cellular heterogeneity, it remained unclear which specific cell types express <em>GSSs</em> and whether exposure to different field hosts triggers distinct differentiation fates in these cells. To address these questions, we constructed a high-resolution atlas of 28,451 midgut cells from larvae reared on radish and pea leaves. Marker gene-based clustering identified 16 distinct cell types, including enterocytes (ECs), enteroendocrine cells (EEs), and intestinal stem cells (ISCs). ScRNA-seq and qRT-PCR analyses revealed host-dependent differential expression patterns of three <em>GSS</em> genes (<em>GSS1-GSS3</em>) among ECs, EEs, and ISCs. Notably, elevated GSS3 protein levels in radish-fed larvae were confirmed by Western blot (WB) and Immunohistochemistry (IHC), while GSS1 and GSS2 exhibited more variable expression patterns. Pseudo-time trajectory analysis further demonstrated that all three <em>GSSs</em> followed differentiation pathways from ISCs into EEs or ECs, but dietary conditions led to distinct differentiation trajectories. This study elucidates the diet-dependent regulatory landscape of <em>GSSs</em> in <em>P. xylostella</em> and delineates the differentiation trajectory of <em>GSS</em>-expressing cells.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104399"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0