Insect Biochemistry and Molecular Biology最新文献

20-hydroxyecdysone regulates the expression of antimicrobial peptides through Dorsal and Relish in Helicoverpa armigera. 20-羟基蜕皮激素通过棉铃虫背侧和津津乐道调节抗菌肽的表达。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-30 DOI: 10.1016/j.ibmb.2025.104378

Caihua Chen, Youying Yan, Jiaxin Wang, Lizhen Zhou, Zhiqiang Lu

{"title":"20-hydroxyecdysone regulates the expression of antimicrobial peptides through Dorsal and Relish in Helicoverpa armigera.","authors":"Caihua Chen, Youying Yan, Jiaxin Wang, Lizhen Zhou, Zhiqiang Lu","doi":"10.1016/j.ibmb.2025.104378","DOIUrl":"https://doi.org/10.1016/j.ibmb.2025.104378","url":null,"abstract":"20-hydroxyecdysone (20E) plays a key role in insect development, not only regulating molting and metamorphosis but also participating in the innate immune responses. The regulation of immunity by 20E varies among different insect species. The pathway and mechanism of 20E regulates immunity in the cotton bollworm (Helicoverpa armigera) remain unclear. In this study, we confirmed that the enhanced immunity is regulated by 20E in H. armigera. 20E upregulates the expression of antimicrobial peptides (AMPs). Knockdown of Dorsal suppressed the 20E-induced expression of AMPs, while knockdown of Relish similarly inhibited the expression of AMPs except Moricin. Overexpression of Dorsal promotes its nuclear translocation and activates the expression of AMPs. However, overexpression of Cactus (IκB-like protein, the inhibitor of NFκB) isoform A binds to Dorsal and inhibits its nuclear translocation. 20E upregulates Dorsal expression via direct EcR mediation and facilitates Dorsal release through phosphorylation of Cactus A. We also report spontaneous activation of HaRelish through auto-proteolytic cleavage, liberating its N-terminal domain (Rel-N). Nuclear Rel-N interacts with Dorsal to co-regulate AMP transcription. These data indicate that 20E induces the expression of AMPs in H. armigera through activation of Dorsal and Relish, thereby enhancing immunity during the metamorphosis stage.","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":" ","pages":"104378"},"PeriodicalIF":3.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The arthropod P450 Enchiridion: An integrated web resource for research on P450s. 节肢动物P450百科：一个用于研究P450的综合网络资源。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-24 DOI: 10.1016/j.ibmb.2025.104377

Jason Charamis, Wannes Dermauw, Thomas Van Leeuwen, John Vontas, René Feyereisen

{"title":"The arthropod P450 Enchiridion: An integrated web resource for research on P450s.","authors":"Jason Charamis, Wannes Dermauw, Thomas Van Leeuwen, John Vontas, René Feyereisen","doi":"10.1016/j.ibmb.2025.104377","DOIUrl":"10.1016/j.ibmb.2025.104377","url":null,"abstract":"We present the Arthropod P450 Enchiridion (arthropodp450.eu), a website based on the DokuWiki architecture and designed to provide resources on structures and functions of CYP genes and P450 enzymes from the most diverse and numerous animals, the arthropods. Interlinked pages cover some essential common features of P450 enzymes from rules of nomenclature to enzyme mechanism and protein structure. Specific to arthropods, lookup tables allow the rapid assignment of currently 1308 CYP families to their nine respective CYP clans, as well as the distribution of P450s from full CYPomes into clans. Manually annotated CYPomes from currently 75 species are presented with their correct CYP names as well as nearly 3000 full-length, named insect transcripts. The Arthropod P450 Enchiridion offers a BLAST function addressing over 11,000 accurately named P450 protein sequences. P450 functions as assessed by in vitro heterologous expression, RNAi phenotype, transgenic in vivo expression, or the phenotype of null mutants are presented for over 1000 cases. The data show as expected a focus on insect and mite pest species, but also highlights the remaining gaps in our knowledge of sequence and function of P450s in less studied groups of arthropods. The architecture of the DokuWiki enables regular updates and expansions to which authors are welcome to contribute.","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":" ","pages":"104377"},"PeriodicalIF":3.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of vesicular glutamate transporter in Chilo suppressalis: Implications for glutamate transport and pest management. 抑制辣椒水疱谷氨酸转运蛋白的功能分析：谷氨酸转运和害虫管理的意义。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-23 DOI: 10.1016/j.ibmb.2025.104376

Xin Mao, Yichi Zhang, Qiwen Pu, Ali Hasnain, Xinyu Liu, Guanghua Luo, Chunqing Zhao

{"title":"Functional analysis of vesicular glutamate transporter in Chilo suppressalis: Implications for glutamate transport and pest management.","authors":"Xin Mao, Yichi Zhang, Qiwen Pu, Ali Hasnain, Xinyu Liu, Guanghua Luo, Chunqing Zhao","doi":"10.1016/j.ibmb.2025.104376","DOIUrl":"10.1016/j.ibmb.2025.104376","url":null,"abstract":"Glutamate is the primary excitatory neurotransmitter in animals, and its storage and release are regulated by vesicular glutamate transporters (vGluTs) in mammals. However, the function of vGluTs in agricultural pests remains largely unexplored. In this study, we successfully cloned the full-length vGluT gene from the rice striped stem borer (RSB) Chilo suppressalis, a major rice pest. Multiple sequence alignment revealed that the amino acid sequence of CsvGluT shared more than 69.82 % similarity with other known insect vGluTs. In the phylogenetic tree, CsvGluT was clustered into a clade with the Asian corn borer Ostrinia furnacalis, which also belongs to Crambidae family. Spatial-temporal expression analysis showed that CsvGluT was highly expressed in the larval heads and abundant in the 1st- and 2nd-instar larvae, whose expression levels are more than three-fold higher than those in the 3rd- to 6th -instar larvae. Electrophysiological recordings indicated that CsvGluT exhibited functional properties like mammalian vGluTs under high concentrations of protons and chloride ions. Furthermore, inhibitors of mammalian vGluTs, including evans blue (EB) and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), significantly inhibited CsvGluT activity. RNA interference (RNAi)-mediated knockdown of CsvGluT significantly reduced the survival rate of RSB to 66 %, highlighting its physiological role and a potential target for RNAi-based RSB control. In summary, our findings elucidated the physiological function of vGluT in RSB and provide a theoretical foundation for advanced research in the development of RNAi-based transporter-targeted strategies for pest management.","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":" ","pages":"104376"},"PeriodicalIF":3.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cullin1 orchestrates insulin/mTOR signaling to drive endocycle progression and ecdysteroid production in Drosophila prothoracic glands during critical weight attainment 在果蝇达到临界体重时，Cullin1调控胰岛素/mTOR信号传导，驱动前胸腺内循环进程和表皮甾体生成。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-19 DOI: 10.1016/j.ibmb.2025.104374

Xiangyan Xue, Zonghao Liu, Ze Zhang, Wei Sun

{"title":"Cullin1 orchestrates insulin/mTOR signaling to drive endocycle progression and ecdysteroid production in Drosophila prothoracic glands during critical weight attainment","authors":"Xiangyan Xue, Zonghao Liu, Ze Zhang, Wei Sun","doi":"10.1016/j.ibmb.2025.104374","DOIUrl":"10.1016/j.ibmb.2025.104374","url":null,"abstract":"<div><div>Critical weight (CW) is a key developmental threshold in insects, beyond which larvae initiate the transformation into pupae. The prothoracic gland (PG), responsible for producing ecdysteroids, plays a crucial role in controlling the timing of this transition. The nutrition dependent endocycle, a modified cell cycle that omits mitosis, coordinates the PG size and activity to influence the timing of CW attainment. However, the molecular mechanisms underlying how nutrient signals determine the endocycle process in PG cells are still not fully uncovered. In this study, we found a conserved SCF (SkpA-Cullin1-Slmb) E3 ligase complex that plays a critical role in regulating endocycle events in Drosophila melanogaster PG cells. Functional disruption of cullin1 (cul1), a core component of this complex, could cause endocycle inhibition, decrease the biosynthesis of ecdysteriod and developmental arrest. The phenotype can be rescued by overexpression cyclin E which may induce rounds of endocycles in the steroidogenic tissue. Remarkably, Cul1 was highly expressed during the CW checkpoint. Starvation before the CW period could repress its expression. In line with this, loss of insulin or target of rapamycin (TOR) signaling could significantly decrease the Cul1 signal during around CW indicating the gene is a nutrient responsive gene. Taken together, our data revealed that Cul1 could serve as downstream regulator of insulin/mTOR signaling pathway to determine the endocycling process around CW in PG cells.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"183 ","pages":"Article 104374"},"PeriodicalIF":3.2,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Killing of Anopheles stephensi mosquitoes by selective triketone inhibitors of 4-hydroxyphenylpyruvate dioxygenase depends on a high protein meal. 选择性4-羟基苯基丙酮酸三酮双加氧酶抑制剂杀灭斯氏按蚊依赖于高蛋白餐。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-18 DOI: 10.1016/j.ibmb.2025.104361

Matěj Kučera, David Hartmann, James J Valdés, Adéla Palusová, Avinash Sheshachalam, Marnix Vlot, Martijn W Vos, Koen J Dechering, Jan Perner

{"title":"Killing of Anopheles stephensi mosquitoes by selective triketone inhibitors of 4-hydroxyphenylpyruvate dioxygenase depends on a high protein meal.","authors":"Matěj Kučera, David Hartmann, James J Valdés, Adéla Palusová, Avinash Sheshachalam, Marnix Vlot, Martijn W Vos, Koen J Dechering, Jan Perner","doi":"10.1016/j.ibmb.2025.104361","DOIUrl":"10.1016/j.ibmb.2025.104361","url":null,"abstract":"The malaria vector Anopheles stephensi has expanded from Asia into Eastern Africa, posing a growing global health threat due to its adaptive biology and increasing resistance to conventional control methods. Here, we characterise 4-hydroxyphenylpyruvate dioxygenase (HPPD), a crucial enzyme in the tyrosine degradation pathway, and demonstrate its potential as a novel drug target in An. stephensi. Homology modeling combined with molecular dynamics simulations confirmed that key inhibitor-binding residues are highly conserved across mosquito HPPDs and predicted potent inhibition by triketone-based compounds. Using cell-based assay with codon-optimized recombinant expression in Escherichia coli, we screened several triketone and diketonitrile HPPD inhibitors and identified nitisinone as the most potent inhibitor, displaying nanomolar-range IC50 values. Membrane feeding assays showed that nitisinone's insecticidal activity relies on ingestion of a high-protein meal, with haemoglobin identified as the potent dietary factor driving toxicity. These results highlight HPPD inhibition as a promising blood-meal-dependent vector control strategy specifically targeting haematophagous mosquitoes.","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":" ","pages":"104361"},"PeriodicalIF":3.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endocrine responses in the pheromone induction of male sexual maturation in an insect 昆虫信息素诱导雄性性成熟过程中的内分泌反应。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-18 DOI: 10.1016/j.ibmb.2025.104365

Stéphane Debernard , Paleo Aguilar , Annick Maria , Annabelle Fuentes , Philippe Couzi , Françoise Bozzolan , Edmundo Gassias , Evan Force

{"title":"Endocrine responses in the pheromone induction of male sexual maturation in an insect","authors":"Stéphane Debernard , Paleo Aguilar , Annick Maria , Annabelle Fuentes , Philippe Couzi , Françoise Bozzolan , Edmundo Gassias , Evan Force","doi":"10.1016/j.ibmb.2025.104365","DOIUrl":"10.1016/j.ibmb.2025.104365","url":null,"abstract":"<div><div>In animals, sexual maturation is marked by the development of reproductive behaviors in synchronism with the acquisition of fertility, and this timing is influenced by chemosensory experiences. In naïve and immature individuals, exposure to sex pheromones may accelerate sexual development, and mechanisms underlying this pheromone induction are not fully identified. Using the moth Agrotis ipsilon, we showed that pre-exposure of immature males to female sex pheromones led to early increases in the performance of sex pheromone-triggered oriented flight as well as in the maturation of accessory sex glands (ASGs) producing seminal proteins. Conjointly, biosynthesis and circulating amounts of juvenile hormone (JH) raised with an upregulation of the expression of JH receptor, Methoprene-tolerant 1 (Met1) and the JH-inducible transcription factor, Krüppel homolog 1 (Kr-h1) in ASGs and the primary olfactory centers, the antennal lobes (ALs). In the sex pheromone pre-exposed immature males, the loss of function of Met1 or Kr-h1 caused a reduction in the induction of the sex pheromone behavioral responsiveness and the ASG secretory activity. Taken together, our results showed that the accelerated effects of sex pheromone pre-exposure on male sexual maturation are mediated by increased JH biosynthesis. This ultimately leads to early induction of JH signaling in ASGs for seminal protein production and in ALs for the central processing of pheromone information, which causes the display of sexual behavior in male A. ipsilon. Finally, this study expands our understanding of endocrine mechanisms by which animals can modulate their fitness according to past olfactory experiences.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"183 ","pages":"Article 104365"},"PeriodicalIF":3.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The evolutionary history of the Methyltransf_FA domain and its role in JH signal in silkworm, Bombyx mori 家蚕甲基转移fa结构域的进化历史及其在JH信号中的作用

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-17 DOI: 10.1016/j.ibmb.2025.104375

Chunyang Wang , Yang Yu , Qi Shen , Rong Xiong , Meiwei Guo , Jiamei Zhao , Dongsheng Yan , Rongcheng Zhang , Ping Chen

{"title":"The evolutionary history of the Methyltransf_FA domain and its role in JH signal in silkworm, Bombyx mori","authors":"Chunyang Wang , Yang Yu , Qi Shen , Rong Xiong , Meiwei Guo , Jiamei Zhao , Dongsheng Yan , Rongcheng Zhang , Ping Chen","doi":"10.1016/j.ibmb.2025.104375","DOIUrl":"10.1016/j.ibmb.2025.104375","url":null,"abstract":"<div><div>The Methyltransf_farnesoic acid (MtFA) domain is a characteristic domain of farnesoic acid O-methyltransferase (FAMeT). FAMeT serves as a key enzyme in crustaceans, catalyzing the rate-limiting step in the biosynthesis of methyl farnesoate (MF). Although homologs of FAMeT have been widely identified in insects, their role in the juvenile hormone (JH) signaling pathway remains unclear. Moreover, the origin and evolutionary history of the MtFA domain remain poorly understood. Interestingly, insect FAMeT contains the MtFA domain and the DUF3421 domain of unknown function. In this study, we found that the MtFA domain was widely distributed across diverse taxa, including protists, vertebrates, and angiosperms. MtFA domain–containing genes likely originated from the common ancestor of prokaryotes and eukaryotes but were lost in archaea. The clustering of MtFA sequences was generally consistent with the classification of MtFA fusion proteins, indicating that domain variation might have been related to protein type. Overexpression of BmFAMeT-2 (which encodes a protein containing 2 MtFA domains and 1 DUF3421 domain) or BmFAMeT-1 (which encodes a protein containing 1 MtFA domain and 1 DUF3421 domain) led to the upregulation of BmKr-h1 expression. In contrast, overexpression of BGIBMGA006319 (encoding a protein containing only 1 DUF3421 domain) led to the downregulation of BmKr-h1 expression. Knockdown of BGIBMGA006319 led to an increase in JH titers, whereas knockdown of BmFAMeT-1 or BmFAMeT-2 resulted in a decrease, with the effect being more pronounced for BmFAMeT-2 than for BmFAMeT-1. These findings suggest that the MtFA domain plays a promoting role in JH signaling, while the DUF3421 domain plays an inhibitory role in JH signaling in silkworms.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"183 ","pages":"Article 104375"},"PeriodicalIF":3.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of the zasp52 gene detected by CRISPR/Cas9 in the global fruit borer Grapholita molesta 利用CRISPR/Cas9技术检测全球果螟zasp52基因的功能

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-16 DOI: 10.1016/j.ibmb.2025.104363

Sha Su , Zhimin Xu , Jinbo Suo , Yurong Zhou , Xiaohe Zhang , Amedius Dwigo Ignatus , Yayun Zuo , Xiong Peng , Fei Li , Maohua Chen

{"title":"Function of the zasp52 gene detected by CRISPR/Cas9 in the global fruit borer Grapholita molesta","authors":"Sha Su , Zhimin Xu , Jinbo Suo , Yurong Zhou , Xiaohe Zhang , Amedius Dwigo Ignatus , Yayun Zuo , Xiong Peng , Fei Li , Maohua Chen","doi":"10.1016/j.ibmb.2025.104363","DOIUrl":"10.1016/j.ibmb.2025.104363","url":null,"abstract":"<div><div>Zasp (Z band alternatively spliced PDZ-motif protein) is the core component of the Z-disc in muscle tissue and plays a vital role in the assembly and maintenance of myofibrils. The zasp has been studied in vertebrates, but it has only been reported in model organisms (e.g. Drosophila melanogaster) among insect species. Grapholita molesta is an important fruit pest with weak flight capacity. Flight capacity is important for the seasonal host switch of this pest. So far, the genes involved in the flight of G. molesta have not been analyzed. In this study, we identified and cloned the Gmzasp52 gene from G. molesta. This gene was expressed in different tissues and developmental stages of G. molesta. Using CRISPR/Cas9 gene editing technology, a homozygous Gmzasp52 gene knockout strain of G. molesta was successfully constructed. Knockout of the Gmzasp52 gene significantly prolonged the larval and preoviposition periods of G. molesta, and shortened the pupal period; the larval weight, pupal weight and fecundity decreased significantly, and the pupal mortality increased significantly; the Z-disc structure of the flight muscle was destroyed, and the myofibrils were damaged, resulting in a significant decrease in flight capacity. These results indicate that the Gmzasp52 plays an important role in the growth and development, reproduction, flight muscle structure and flight capacity of G. molesta. Our study reveals the role of zasp52 gene in a non-model insect species for the first time. The results not only analyzed the role of Gmzasp52 gene in G. molesta, but also further characterized the function of zasp52 gene in non-model organisms and provided possible target gene for the control of this pest.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"183 ","pages":"Article 104363"},"PeriodicalIF":3.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome assembly of Diadegma fenestrale (Hymenoptera: Ichneumonidae), and genome integration of its symbiotic virus, DfIV 姬蜂（膜翅目：姬蜂科）基因组组装及其共生病毒DfIV基因组整合

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-16 DOI: 10.1016/j.ibmb.2025.104366

Juil Kim , Murtaza Khan

{"title":"Genome assembly of Diadegma fenestrale (Hymenoptera: Ichneumonidae), and genome integration of its symbiotic virus, DfIV","authors":"Juil Kim , Murtaza Khan","doi":"10.1016/j.ibmb.2025.104366","DOIUrl":"10.1016/j.ibmb.2025.104366","url":null,"abstract":"<div><div>Diadegma fenestrale is a parasitic wasp of ecological and agricultural significance, regulating pest populations. However, limited genomic resources have hindered a deeper understanding of its biology and symbiotic interactions. This study presents a chromosome-level genome assembly of D. fenestrale using Nanopore and Illumina sequencing. The assembled 221.1 Mb genome comprises 68 scaffolds, including 11 at the chromosomal level, and exhibits high completeness with a BUSCO completeness score of 99.6 %. A total of 13,544 protein-coding genes were predicted, with BUSCO assessment of the gene set indicating 97.5 % completeness (single-copy: 96.8 %, duplicated: 0.7 %), 0.7 % fragmented, and 1.8 % missing genes. Comparative genomic analysis with closely related hymenopteran species provides new insights into genome evolution, including gene family expansion, contraction patterns, and chromosomal rearrangements. Additionally, this study examines DfIV, a symbiotic virus associated with D. fenestrale, identifying 62 genome segments integrated into the host genome. Most segments are present in one or two copies, while four segments exhibit three copies, suggesting a dynamic interaction between the virus and the host genome that may influence gene regulation and chromosomal stability. This study provides a comprehensive genomic resource for D. fenestrale, enhancing our understanding of its genomic architecture, evolutionary dynamics, and functional adaptations. The findings contribute to broader research on parasitoid wasps, and symbiotic virus-host interactions, with implications for biological pest control and evolutionary biology.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"183 ","pages":"Article 104366"},"PeriodicalIF":3.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melanization immune response of Spodoptera frugiperda to an endoparasitoid Microplitis pallidipes 夜蛾对类内寄生虫苍白微囊炎的黑化免疫反应

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-07-16 DOI: 10.1016/j.ibmb.2025.104364

Hao Zhang , Jin-Yan Wang , Lan-Lan Huang , Chris Bass , Jun-Xiang Zhou , Yi-Juan Chen , Jie-Xian Jiang , Xiang-Yun Ji , Nian-Feng Wan

{"title":"Melanization immune response of Spodoptera frugiperda to an endoparasitoid Microplitis pallidipes","authors":"Hao Zhang , Jin-Yan Wang , Lan-Lan Huang , Chris Bass , Jun-Xiang Zhou , Yi-Juan Chen , Jie-Xian Jiang , Xiang-Yun Ji , Nian-Feng Wan","doi":"10.1016/j.ibmb.2025.104364","DOIUrl":"10.1016/j.ibmb.2025.104364","url":null,"abstract":"<div><div>Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) is a worldwide invasive pest. Microplitis pallidipes Szépligeti (Hymenoptera: Braconidae) is an endoparasitoid of this pest. However, the melanization immune response of this pest to M. pallidipes, has not yet been explored. We found that the parasitism of S. frugiperda by M. pallidipes severely inhibited hemolymph melanization and phenoloxidase activity of larvae. Transcriptomic and proteomic analysis indicated that 1128 genes and 594 proteins were significantly altered in expression following parasitism. These included 12 of the 23 candidate genes involved in melanization in S. frugiperda (i.e., 7 recognition protein genes, 10 prophenoloxidase (PPO) cascade genes, and 6 melanin synthesis genes). Eight of these 12 melanization genes were significantly downregulated in S. frugiperda larvae 24 h or 48 h after treatment with siRNAs. Of these, RNAi knockdown of PPO1, PPO2 and TH (tyrosine hydroxylase) increased parasitism rate by 13.3 %–23.3 % and decreased hemymphatic melanization rate by 36.7–66.7 %. Our results provide insight into the melanization response of S. frugiperda and identify genes that appear to play a key role in this process. These findings shed light on the mechanisms by which insect herbivores respond to parasitism, and have applied implications for the biological control of insect pests by parasitic wasps.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"183 ","pages":"Article 104364"},"PeriodicalIF":3.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0