Insect Biochemistry and Molecular Biology最新文献

{"title":"Annotation of transcription factors, chromatin-associated factors, and basal transcription machinery in the pea aphid, Acyrthosiphon pisum, and development of the ATFdb database, a resource for studies of transcriptional regulation","authors":"Nicolas Parisot ,&nbsp;Mélanie Ribeiro Lopes ,&nbsp;Sergio Peignier ,&nbsp;Patrice Baa-Puyoulet ,&nbsp;Hubert Charles ,&nbsp;Federica Calevro ,&nbsp;Patrick Callaerts","doi":"10.1016/j.ibmb.2024.104217","DOIUrl":"10.1016/j.ibmb.2024.104217","url":null,"abstract":"<div><div>The pea aphid, <em>Acyrthosiphon pisum,</em> is an emerging model system in functional and comparative genomics, in part due to the availability of new genomic approaches and the different sequencing and annotation efforts that the community has dedicated to this important crop pest insect. The pea aphid is also used as a model to study fascinating biological traits of aphids, such as their extensive polyphenisms, their bacteriocyte-confined nutritional symbiosis, or their adaptation to the highly unbalanced diet represented by phloem sap. To get insights into the molecular basis of all these processes, it is important to have an appropriate annotation of transcription factors (TFs), which would enable the reconstruction/inference of gene regulatory networks in aphids. Using the latest version of the <em>A. pisum</em> genome assembly and annotation, which represents the first chromosome-level pea aphid genome, we annotated the complete repertoire of <em>A. pisum</em> TFs and complemented this information by annotating genes encoding chromatin-associated and basal transcription machinery proteins. These annotations were done combining information from the model <em>Drosophila melanogaster</em>, for which we also provide a revisited list of these proteins, and <em>de novo</em> prediction. The comparison between the two model systems allowed the identification of major losses or expansions in each genome, while a deeper analysis was made of ZNF TFs (with certain families expanded in the pea aphid), and the Hox gene cluster (showing reorganization in gene position in the pea aphid compared to <em>D. melanogaster</em>). All annotations are available to the community through the Aphid Transcription Factors database (ATFdb), consolidating the various annotations we generated. ATFdb serves as a valuable resource for gene regulation studies in aphids.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104217"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet influence on male sexual maturation through interplay between insulin signaling and juvenile hormone in insects","authors":"Evan Force ,&nbsp;Claudia Alvarez ,&nbsp;Annabelle Fuentes ,&nbsp;Annick Maria ,&nbsp;Françoise Bozzolan ,&nbsp;Stéphane Debernard","doi":"10.1016/j.ibmb.2024.104252","DOIUrl":"10.1016/j.ibmb.2024.104252","url":null,"abstract":"<div><div>In animals, sexual maturation coincides with the development of sexual behaviors and reproductive system. These developmental events are influenced by diet and governed by endocrine signals. Here, for the first time in insects, we explored functional links between nutrition and juvenile hormone (JH) in the male reproductive physiology through the insulin signaling pathway (ISP) acting as a transducer of nutritional signals. We turned to the male moth <em>Agrotis ipsilon</em> for which sexual maturation, including accessory sex glands (ASGs) development concomitantly with antennal lobes (ALs) maturation for female sex pheromone processing and display of sexual behavior, is known to be JH- and diet-dependent. Indeed, a diet rich in sugars with sodium was previously shown to accelerate sexual maturation, which was achieved from the third day of adult life. In this study, we demonstrated that such a diet raised i) the expression of JH signaling actors (Methoprene-tolerant, Taiman, and Krüppel homolog 1) in ALs and ASGs, ii) the biosynthesis and circulating levels of JH, and iii) the expression of both <em>insulin receptor</em> (<em>InR</em>) and <em>insulin-like peptides</em> (<em>ILPs</em>) in <em>corpora allata</em> (CAs) and brain respectively. Insulin injection raised JH biosynthesis following increased <em>HMG-CoA reductase</em> expression in CAs; opposite effects were induced in InR-deficient males. Thus, we highlighted that promoting effects of a diet composed of sugars with sodium on male sexual maturation results from an early induction of ISP causing an increase in JH biosynthesis followed by a potentiation of JH actions on the development of ASGs and ALs in <em>A. ipsilon</em>.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104252"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal fluid proteins in the Queensland fruit fly: Tissue origins, effects of mating and comparative genomics","authors":"Khandaker Asif Ahmed ,&nbsp;Heng Lin Yeap ,&nbsp;Chris W. Coppin ,&nbsp;Jian-Wei Liu ,&nbsp;Gunjan Pandey ,&nbsp;Phillip W. Taylor ,&nbsp;Siu Fai Lee ,&nbsp;John G. Oakeshott","doi":"10.1016/j.ibmb.2024.104247","DOIUrl":"10.1016/j.ibmb.2024.104247","url":null,"abstract":"<div><div>In many insect species, the ability of males to inhibit their mates from remating is an important component of fitness. This ability is also essential for the effective management of insect pests, including tephritid fruit flies, using the Sterile Insect Technique. Here we apply transcriptomics and proteomics to male reproductive tissues before and after mating to characterize components of semen that might mediate remating inhibition in Queensland fruit fly. We found 144 genes whose transcripts were enriched, or proteins expressed, in reproductive tissue and which also varied in amount after mating. Some were associated with testes, accessory glands and ejaculatory apodeme, but those from the ejaculatory apodeme were over-represented compared to those not enriched in reproductive tissue or mating responsive. These included 13 related genes clustered within one Mb on chromosome 5. Functional annotations implicated a broad range of biochemical processes in the genes/proteins enriched in reproductive tissue and mating responsive, with cuticle structure most commonly implicated among the subset of these that were apodeme-enriched and a kinase involved in vitellogenesis implicated for one of the 13 clustered genes. We did not find a homolog of the much studied <em>Drosophila melanogaster</em> Sex Peptide but comparative genomics indicated that some of the tissue-enriched, mating responsive genes/proteins were rapidly evolving in tephritids (including in the Queensland fruit fly lineage), suggesting recent adaptation to new functional niches. Our results provide a set of candidate mediators of remating inhibition for further functional testing.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104247"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β2-tubulin and its promoter in the brown planthopper: A versatile tool for genetic control strategies","authors":"Jing-Xiang Chen ,&nbsp;Chuan-Chuan Zhang ,&nbsp;Jia-Wei Sun ,&nbsp;Yi-Bing Zhang,&nbsp;Min-Shi Luo,&nbsp;Wen-Qing Zhang","doi":"10.1016/j.ibmb.2024.104244","DOIUrl":"10.1016/j.ibmb.2024.104244","url":null,"abstract":"<div><div>At present, the application of CRISPR/Cas9 technology for genetic manipulation in insects is predominantly concentrated on Diptera model species, including <em>Drosophila</em> and mosquitoes. In contrast, non-model insects such as the brown planthoppers (BPH, <em>Nilaparvata lugens</em>), a major insect pest of rice, have received less attention in genetic manipulation due to insufficient tools. Here, the analysis of spatiotemporal expression patterns revealed that <em>β2-tubulin</em> in BPH (<em>NlB2t</em>) was predominantly concentrated in male adults and male testis, exhibiting high expression levels. Knockdown of <em>NlB2t</em> expression by using RNAi resulted in the obstruction of male testis development. Mating between the RNAi-treated males and wild-type females led to a notable reduction in the number of eggs laid and the hatching rate of those eggs by 58.2% and 50.6%, respectively. The longevity of RNAi males significantly increased, and females that had previously mated with RNAi males exhibited a diminished inclination for re-mating with wild-type males. The dual-luciferase reporter assay demonstrated robust promoter activity in the upstream 943 bp of <em>NlB2t</em>, capable of driving Cas9 protein expression <em>in vivo</em> and effectively inducing target gene knockout. These findings elucidated that <em>NlB2t</em> may be a key gene in BPH male testis development and reproduction, as a promising target for sterilization. Its upstream promoter serves as a germline promoter, significantly facilitating the development of genetic control tools based on CRISPR/Cas9 technology in BPH.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104244"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of the clock gene period in regulating circadian rhythm of courtship vibrations in Nilaparvata lugens","authors":"Qi Wei,&nbsp;Jia-Chun He,&nbsp;Wei-Xia Wang,&nbsp;Feng-Xiang Lai,&nbsp;Pin-Jun Wan,&nbsp;Qiang Fu","doi":"10.1016/j.ibmb.2024.104250","DOIUrl":"10.1016/j.ibmb.2024.104250","url":null,"abstract":"<div><div><em>Nilaparvata lugens</em>, the brown planthopper (BPH), is a notorious pest threatening rice production across Asia. The heavy reliance on synthetic insecticides for control has led to resistance and raised ecological concerns. Substrate-borne vibrational communication, integral to species-specific mate recognition systems in insects, presents a potential avenue for pest management through mating disruption. However, the molecular mechanisms regulating vibrational signals in BPH remain poorly understood. In this study, we cloned and analyzed the clock gene <em>period</em> from BPH. The open reading frame of <em>Nlper</em> is 3708 bp, encoding a 1235-amino acid protein with two conserved domains: the Per-ARNT-Sim domain and the Period protein 2/3C-terminal region. It shares a closer evolutionary relationship with <em>Laodelphax striatellus</em> and <em>Frankliniella occidentalis</em>. Spatiotemporal expression analysis showed that <em>Nlper</em> was consistently expressed across all life stages and adult tissues, with the highest levels in macropterous males and male head, respectively. Rhythmic expression exhibited significant circadian oscillations under both light-dark and constant darkness conditions, peaking at 00:00 and reaching a trough at 12:00, with fold changes ranging from 2.47 to 3.39. Moreover, after dsRNA injection, <em>Nlper</em> expression decreased by 77.21%–84.26% from day 2 to day 5, disrupting the circadian oscillation of female vibrational signals (FVS) and causing a significant peak shift, along with a 30.56% reduction in FVS frequency on day 5. These findings underscore the essential role of <em>Nlper</em> in regulating the circadian rhythm of courtship vibrational signals, deepening our understanding of the genetic basis of insect communication and opening new possibilities for innovative pest management approaches.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104250"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nap1 is essential for eupyrene spermatogenesis and migration in Plutella xylostella","authors":"Chenxu Zhu ,&nbsp;Lijun Zhou ,&nbsp;Dongbin Chen ,&nbsp;Xu Yang ,&nbsp;Lu Zhu ,&nbsp;Lansa Qian ,&nbsp;Xiaomiao Xu ,&nbsp;Gangqi Fang ,&nbsp;Awawing A. Andongma ,&nbsp;Yongcheng Dong ,&nbsp;Lin He ,&nbsp;Yongping Huang ,&nbsp;Yaohui Wang","doi":"10.1016/j.ibmb.2024.104245","DOIUrl":"10.1016/j.ibmb.2024.104245","url":null,"abstract":"<div><div>Spermatogenesis is a key process for the sexual reproduction species. In lepidopteran insects, spermatogenesis produces two different types of sperms, in which eupyrene sperm carry genomic DNA and fertilize eggs, whereas apyrene sperm are necessary for eupyrene sperm to enter eggs. However, functional genetic studies of spermatogenesis in <em>Plutella xylostella</em> remain a longstanding puzzle even though the phenomenon in lepidoptera has been widely documented more than a century. In this study, we particularly focus on the gene <em>Nap1</em> which belongs to the Nucleosome assembly protein family. Our findings revealed that <em>Nap1</em> was highly expressed in the testes, and the disruption of <em>PxNap1</em> induced male sterility in <em>P. xylostella</em>, while the fertility of mutant females was comparable to wild-type females. Additionally, through immunofluorescence staining analysis, we found that the eupyrene sperm bundles presented diffusedly scattered nuclei in <em>PxNap1</em> mutant males, while the nuclei in the wild-type were clustered together presented as needle shape. We also found that <em>PxNap1</em> deficiency hinders the transfer of eupyrene sperm to the bursa copulatrix and spermatheca of females. However, the apyrene spermatogenesis was not affected in the <em>PxNap1</em> mutant. RNA-seq analyses indicated that the defects of eupyrene sperm in <em>PxNap1</em> mutants were related to energy metabolic such as pentose and glucuronate interconversions, biosynthesis of amino acids, and pentose phosphate pathway. Our study demonstrates that <em>PxNap1</em> plays crucial function in eupyrene spermatogenesis and eupyrene sperm migration. Our research provides valuable insights for the genetic factors underlying reproductive processes in Lepidopteran insects.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104245"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Juvenile hormone regulates reproductive diapause through both canonical and noncanonical pathways in the bean bug Riptortus pedestris","authors":"Abdul Hafeez ,&nbsp;Kou Wang ,&nbsp;Wen Liu ,&nbsp;Xiao-Ping Wang","doi":"10.1016/j.ibmb.2024.104233","DOIUrl":"10.1016/j.ibmb.2024.104233","url":null,"abstract":"<div><div>Diapause is an adaptive developmental arrest commonly utilized by animals to cope with seasonal changes. Central to this process are hormonal events that bridge photoperiodic cues and physiological changes. In insect reproductive diapause, the absence of juvenile hormone (JH) serves as the primary endocrine event that governs key diapause traits, including ovarian developmental arrest and lipid accumulation. Conventionally, it is believed that the effects of JH are conveyed through the receptor Methoprene-tolerant (Met) and its transcriptional factor Krüppel homolog 1 (Kr-h1). However, our study with the bean bug <em>Riptortus pedestris</em> reveals that JH independently regulates lipid accumulation, bypassing <em>Met</em> and <em>Kr-h1</em> pathways. <em>R. pedestris</em> enters reproduction under long-day (LD) conditions, while diapause occurs under short-day (SD) conditions. Treatment of SD females with the JH mimic methoprene stimulated reproductive activities, enhancing ovary development and reducing lipid accumulation. In contrast, silencing genes essential for JH biosynthesis in LD females led to pronounced diapause characteristics, including ovarian developmental arrest and substantial lipid accumulation. Interestingly, disruptions in the JH action genes, either <em>Met</em> or <em>Kr-h1</em>, solely affected ovary development, leaving lipid accumulation unchanged, indicating an independent pathway for regulating JH in lipid accumulation. This was further confirmed by RNA interference experiments in SD females, where knockdown of <em>Met</em> or <em>Kr-h1</em> did not alter the effects of methoprene on lipid reduction. Collectively, these results suggest that JH controls ovary development through the established Met-Kr-h1 pathway, while it modulates lipid accumulation through an alternative, yet to be identified noncanonical pathway during reproductive diapause in <em>R. pedestris.</em></div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104233"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs","authors":"A. Mocchetti ,&nbsp;S. De Rouck ,&nbsp;S. Naessens,&nbsp;W. Dermauw,&nbsp;T. Van Leeuwen","doi":"10.1016/j.ibmb.2024.104232","DOIUrl":"10.1016/j.ibmb.2024.104232","url":null,"abstract":"<div><div>Despite the establishment of CRISPR-Cas9 gene editing protocols in a wide range of organisms, genetic engineering is still challenging for many organisms due to constraints including lethality of embryo injection, difficulties in egg/embryo collection or viviparous lifestyles. Recently, an efficient CRISPR-Cas9 method, termed SYNCAS, was developed to genetically modify spider mites and thrips species. The method is based on maternal injection of formulated CRISPR-Cas9 using saponin and BAPC. Here, we investigate whether the method can be used to perform gene editing in other arthropods such as the beneficial predatory mites <em>Amblyseius swirskii</em> and <em>Phytoseiulus persimilis</em>, and the pests <em>Bemisia tabaci</em> and <em>Nezara viridula</em>. For the predatory mites, <em>Antp</em> and <em>SLC25A38</em> were used as target genes, while the ortholog of the <em>Drosophila melanogaster</em> ABCG transporter <em>white</em> was targeted in <em>B. tabaci</em> and <em>N. viridula</em>. All species were successfully edited with the highest efficiencies (up to 39%) being obtained for <em>B. tabaci</em>. For <em>A. swirskii</em> and <em>P. persimilis</em> no clear phenotypes could be observed, even though SLC25A38 was successfully knocked-out. The lack of a color phenotype in SLC25A38 mutants was confirmed in the spider mite <em>Tetranychus urticae</em>. Disruption of the target gene <em>Antp</em> is likely lethal in predatory mites, as no true null mutants could be recovered. For <em>B. tabaci,</em> KO of <em>white</em> resulted in orange eyes which diverges from the phenotype seen in <em>white</em> mutants of <em>D. melanogaster</em>. In the last species, <em>N. viridula,</em> a single phenotypic mutant could be detected having a patchy white body coloration with wild type eye coloration. Genotyping revealed a single amino acid deletion at the target site, suggesting the creation of a hypomorphic allele. To conclude, the protocols provided in this work can contribute to the genetic study of predatory mites used in biological control, as well as hemipteran pests.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104232"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and pharmacological characterization of pH-sensitive chloride channels in the fall armyworm, Spodoptera frugiperda","authors":"Wei Wang ,&nbsp;Jia-Sheng Chen ,&nbsp;Pei-Yun He ,&nbsp;Mo-Han Zhang ,&nbsp;Hai-Qun Cao ,&nbsp;Subba Reddy Palli ,&nbsp;Cheng-Wang Sheng","doi":"10.1016/j.ibmb.2024.104243","DOIUrl":"10.1016/j.ibmb.2024.104243","url":null,"abstract":"<div><div>The pH-sensitive chloride channels (pHCls) are unique to invertebrates and play crucial roles in fluid regulation, food selection, and intake. In this study, we identified and isolated two cDNAs encoding the <em>Sf</em>pHCl1 and <em>Sf</em>pHCl2 subunits from the fall armyworm, <em>Spodoptera frugiperda</em>. Both subunits exhibited similar expression patterns. When expressed in <em>Xenopus laevis</em> oocytes, <em>Sf</em>pHCl1 and <em>Sf</em>pHCl2 formed functional chloride channels with reversal potentials indicative of chloride selectivity. Shifts in extracellular pH from acidic to alkaline conditions induced inward currents in both <em>Sf</em>pHCl1 and <em>Sf</em>pHCl2, with EC₅₀ values of pH 8.24 and 8.49, respectively. Zinc ions (Zn²⁺) and the insecticide emamectin benzoate (EB) also activated concentration-dependent inward currents in these channels, whether expressed individually or co-expressed. Notably, <em>Sf</em>pHCl1 and <em>Sf</em>pHCl2 channels exhibited significant differences in their activation and deactivation time constants. These findings elucidate the biophysical and pharmacological characteristics of pH-sensitive, zinc-gated chloride channels, which, being exclusive to invertebrates, present a promising target for the development of highly specific insecticides.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104243"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expansion of three types of transposon superfamilies within 25 Mya lead to large genome size of a rice insect pest","authors":"Bingbing He ,&nbsp;Yuyang Cong ,&nbsp;Le Xu ,&nbsp;Ying Liu","doi":"10.1016/j.ibmb.2024.104251","DOIUrl":"10.1016/j.ibmb.2024.104251","url":null,"abstract":"<div><div>The brown planthoppers (BPH, <em>Nilaparvata lugens)</em>, white backed planthopper (WBPH, <em>Sogatella furcifera</em>) and small brown planthopper (SBPH, <em>Laodelphax striatellus</em>) are widely distributed rice insect pests, causing huge annual yield loss of rice production. Though these three planthoppers belong to the same family, Delphacidae of Hemiptera, their genome sizes (GS) are very different, ranging from 541 to 1088 Mb. To uncover the main factors driving GS changes of three planthoppers, we first estimated the GS of their ancestor Fulgoroidea, to be 794.33 Mb, indicating GS expansion in BPH but contraction in SBPH and WBPH. Next, we identified repetitive sequences and compared the TE landscapes, showed that three types of transposon superfamilies, hAT, Tc1-Mariner and Gypsy, expanded within 25 Mya in BPH. In addition, BPH kept ancient TEs of Fulgoroidea dated back to 175 Mya, while SBPH and WBPH have lost most of these ancient TEs. Here, we present evidence that the gain of recently expanded TEs driving the GS expansion and loss of ancient TEs leading to the GS contraction, providing new insights into the mechanism of GS variation.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104251"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}