Insect Biochemistry and Molecular Biology最新文献

{"title":"CRISPR/Cas13a-mediated visual detection: A rapid and robust method for early detection of Nosema bombycis in silkworms","authors":"Yi-Xiang Wu ,&nbsp;Samreen Sadiq ,&nbsp;Xin-Hao Jiao ,&nbsp;Xue-Min Zhou ,&nbsp;Lu-Lai Wang ,&nbsp;Xin-Ran Xie ,&nbsp;Iltaf Khan ,&nbsp;Ping Wu","doi":"10.1016/j.ibmb.2024.104203","DOIUrl":"10.1016/j.ibmb.2024.104203","url":null,"abstract":"<div><div>The sericulture industry faces a significant threat from the Pebrine disease of silkworms, caused by <em>Nosema bombycis</em>. Nonetheless, the current microscopic diagnostic methods can be time-consuming, labor-intensive, and lacking sensitivity and accuracy. Therefore, it is crucial to develop a novel detection approach that is efficient, highly sensitive, and low-cost. In this regard, the CRISPR/Cas system has the potential to be a fast, accurate, and highly specific method of detection. Herein, using a microplate reader, a portable fluorescence detection device, and test strips as signal output tools respectively, we have efficiently developed three rapid and facile visual detection methods for <em>N. bombycis</em> using a CRISPR/Cas13a system with conjugation of Recombinase polymerase amplification (RPA). We evaluated the sensitivity of this combined technology by comparing it with the positive plasmid standard and the genome standard of <em>N. bombycis</em>. Remarkably, the sensitivity of the CRISPR/Cas13a system for <em>N. bombycis</em> positive plasmid standard based on the microplate reader, portable fluorescence detection device, and test strips was 1 copy/μL, 10 copies/μL, and 1 copy/μL, respectively, while for the <em>N. bombycis</em> genome standards, the detection sensitivity was 10 fg/μL, 10 fg/μL, and 1 fg/μL, respectively. In addition, extensive evaluations have demonstrated that the established technology can accurately detect <em>N. bombycis</em> without cross-reactivity with other pathogens, ensuring a specificity rate of 100%. In brief, this study will provide a practical, efficient, and affordable method for early and rapid detection of <em>N. bombycis</em> in various settings.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104203"},"PeriodicalIF":3.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larval development of a parasitoid depends on host ecdysteroids","authors":"Xinyi Liu ,&nbsp;Zhe Jiang ,&nbsp;Wei Sun ,&nbsp;Jialei Lu ,&nbsp;Jiaru He ,&nbsp;Yijie Wang ,&nbsp;Fanchi Li ,&nbsp;Bing Li ,&nbsp;Jing Wei","doi":"10.1016/j.ibmb.2024.104195","DOIUrl":"10.1016/j.ibmb.2024.104195","url":null,"abstract":"<div><div>Parasitoids often exhibit high flexibility in their development depending on stages of their host at the parasitism, yet little is known about the mechanism underlying such flexibility. In the study, we evaluated the larval development time of the parasitoid <em>Exorista sorbillans</em> (Diptera: Tachinidae) on the lepidopteran model insect <em>Bombyx mori</em> (Lepidoptera: Bombycidae). We found that the development duration of <em>E. sorbillans</em> larvae parasitizing on the late-developmental silkworms was significantly shorter than that of the larvae parasitizing on the early-developmental hosts. Intriguingly, the 2nd-3rd instar molting of parasitoid always occurred when the ecdysteroid titers in the host were increased to higher levels. Furthermore, inhibiting the release of ecdysteroids to parasitic abdomen by thorax-abdomen ligation of the host only repressed the 2nd-instar growth and molting of <em>E. sorbillans</em> larvae, but had no effect on their pupation. Meanwhile, the ecdysone synthesis and 20-hydroxyecdysone (20 E) signaling in larval parasitoids were impeded after ligation treatment. Moreover, exogenous 20 E application could largely rescue the defect in 2nd instar growth and molting through stimulating ecdysone synthesis and signaling in <em>E. sorbillans</em>. Our results indicate that the parasitoid requires the host ecdysteroids to stimulate 20 E signaling and the subsequent 2nd-instar growth and molting. These findings will improve our understanding of the host utilization strategies of parasitoids, and contribute to the development of <em>in vitro</em> rearing procedures of tachinid parasitoids for biological control.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104195"},"PeriodicalIF":3.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-dependent switched taste behavior to ribose","authors":"Jiun Sang,&nbsp;Youngseok Lee","doi":"10.1016/j.ibmb.2024.104194","DOIUrl":"10.1016/j.ibmb.2024.104194","url":null,"abstract":"<div><div>Chemical detection is vital for animal survival, aiding in avoiding toxins and selecting nutritious foods. While <em>Drosophila</em> larvae exhibit appetitive feeding behavior toward ribose, an important sugar for RNA, nucleotide, and nucleoside synthesis, how adult <em>Drosophila</em> perceives ribose remains unclear. Through behavioral and electrophysiological investigations, we unexpectedly discovered that adult flies actively avoid ribose. Our external electrophysiological analysis revealed that ribose is detected through bitter-sensing gustatory receptor neurons in S-type sensilla, suggesting its perception as a bitter compound. Additionally, we identify <em>painless</em> as crucial for both ribose aversion and the neuronal response to ribose.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104194"},"PeriodicalIF":3.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemolymph protease-17b activates proHP6 to stimulate melanization and Toll signaling in Manduca sexta","authors":"Yang Wang,&nbsp;Haobo Jiang","doi":"10.1016/j.ibmb.2024.104193","DOIUrl":"10.1016/j.ibmb.2024.104193","url":null,"abstract":"<div><div><em>Manduca sexta</em> hemolymph protease-6 (HP6) plays a central role in coordinating antimicrobial responses, such as prophenoloxidase (PPO) activation and Toll signaling. Our previous studies indicated that HP5 and GP6 activate proHP6 in larval hemolymph and extraembryonic tissues, respectively. Here, we report the characterization of HP17b as another HP6 activating enzyme and its regulation by multiple serpins in hemolymph. The precursor of HP17b expressed in baculovirus infected Sf9 cells became spontaneously cleaved at two sites, and these products were purified together in one preparation named HP17b′, a mixture of proHP17b, a 35 kDa intermediate, and HP17b. HP17b′ converted proHP6 to HP6. As reported before, HP6 converted precursors of PPO activating protease-1 (PAP1) and HP8 to their active forms. HP8 activates proSpӓtzle-1 to turn on Toll signaling. We found HP17b′ directly activated proSPHI and II to form a cofactor for PPO activation by PAP1. Supplementation of larval hemolymph with HP17b′, HP17b, or proHP17b significantly increased PPO activation. Adding <em>Micrococcus luteus</em> to the reactions did not enhance PPO activation in the reactions containing HP17b′, HP17b, or proHP17b. Using HP17b antibodies, we isolated from induced plasma HP17b fragments and associated proteins (<em>e.g</em>., serpin-4). Serpin-1A, 1J, 1J′, 4, 5, or 6 reduced the activation of proHP6 by HP17b’ through formation of covalent complexes with active HP17b. We detected an activity for proHP17b cleavage in hemolymph from bar-stage pharate pupae but failed to purify the protease due to its high instability. Other known HPs did not activate proHP17b <em>in vitro</em>. Together, these results suggest that HP17b is a clip-domain protease activated by an unknown endopeptidase in response to a danger signal and regulated by multiple serpins.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104193"},"PeriodicalIF":3.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis reveal the fall armyworm Spodoptera frugiperda tolerate high temperature by mediating chitin-related genes","authors":"Xiaorui Yan ,&nbsp;Zihua Zhao ,&nbsp;Shiqian Feng ,&nbsp;Yongjun Zhang ,&nbsp;Zhenying Wang ,&nbsp;Zhihong Li","doi":"10.1016/j.ibmb.2024.104192","DOIUrl":"10.1016/j.ibmb.2024.104192","url":null,"abstract":"<div><div>Climate change facilitates the rapid invasion of agricultural pests, threatening global food security. The fall armyworm <em>Spodoptera frugiperda</em> is a highly polyphagous migratory pest tolerant to high temperatures, allowing its proliferation in harsh thermal environments. We aimed to demonstrate mechanisms of its high-temperature tolerance, particularly transcriptional and metabolic regulation, which are poorly understood. To achieve the aim, we examined the impact and mechanism of heat events on <em>S. frugiperda</em> by using multiple approaches: ecological measurements, transcriptomics, metabolomics, RNAi, and CRISPR/Cas9 technology. We observed that several physiological indices (larval survival rate, larval period, pupation rate, pupal weight, eclosion rate, and average fecundity) decreased as the temperature increased, with the 32 °C treatment displaying a significant difference from the control group at 26 °C. Significantly upregulated expression of genes encoding endochitinase and chitin deacetylase was observed in the chitin-binding, extracellular region, and carbohydrate metabolic process GO terms of hemolymph, fat body, and brain, exhibiting a tissue-specific pattern. Significantly enriched pathways (e.g., cutin, suberin, and wax biosynthesis; oxidative phosphorylation and cofactor biosynthesis; diverse amino acid biosynthesis and degradation; carbon metabolism; and energy metabolism), all of which are essential for <em>S. frugiperda</em> larvae to tolerate temperature, were found in metabolites that were expressed differently. Successful RNA interference targeting of the three chitin-related genes reduced gene expression levels and larval survival rate. Knockout of the endochitinase gene by using the CRISPR/Cas9 system significantly reduced the relative gene expression and increased sensitivity to high-temperature exposure. On the basis of our findings, theoretical foundations for understanding the high-temperature tolerance of <em>S. frugiperda</em> populations and latent genetic control strategies were established.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104192"},"PeriodicalIF":3.2,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of salivary proteins in the rice leaf folder Cnaphalocrocis medinalis by transcriptome and LC-MS/MS analyses","authors":"Jiarong Cui ,&nbsp;Xianjing Yao ,&nbsp;Zhihan Ni ,&nbsp;Hongfeng Zhao ,&nbsp;Yajun Yang ,&nbsp;Hongxing Xu ,&nbsp;Zhongxian Lu ,&nbsp;Pingyang Zhu","doi":"10.1016/j.ibmb.2024.104191","DOIUrl":"10.1016/j.ibmb.2024.104191","url":null,"abstract":"<div><div>Salivary proteins in the oral secretion (OS) of chewing insects play a crucial role in insect-plant interactions during feeding. The rice leaf folder <em>Cnaphalocrocis medinalis</em>, a notorious pest in global rice production, triggers defense responses during feeding, but little is known about its salivary proteins. In this study, we confirmed that <em>C. medinalis</em> releases OS during feeding. By employing transcriptomic analysis and liquid chromatography-tandem mass spectroscopy (LC-MS/MS), we examined the salivary proteins from labial salivary glands and OS from <em>C. medinalis</em>. A total of 14,397 genes were expressed at the RNA level and 229 salivary proteins were identified. Comparative analysis with other 25 arthropod species revealed that 43 proteins were unique to <em>C. medinalis</em>. Expression pattern analysis revealed that most of the selected genes were highly expressed in the gut and the larval stages (4th–5th instar). These findings provide a comprehensive resource for future functional studies of salivary proteins, offering new insights into the molecular mechanisms by which <em>C. medinalis</em> modulates plant defenses and potential applications in pest management.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104191"},"PeriodicalIF":3.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bipartite nuclear localization sequence is indispensable for nuclear import and stability of self-dimerization of ADARa in Bombyx mori","authors":"Song Jiang ,&nbsp;Junzhe Peng ,&nbsp;Syeda Saneela ,&nbsp;Ruoyun Shi ,&nbsp;Daoming Wang ,&nbsp;Qingheng Tang ,&nbsp;Xiaming Shi ,&nbsp;Yan Meng","doi":"10.1016/j.ibmb.2024.104190","DOIUrl":"10.1016/j.ibmb.2024.104190","url":null,"abstract":"<div><div>The conservative post-transcriptional modification in mammals and <em>Drosophila</em> is adenosine-to-inosine (A-to-I) deamination in double-stranded RNA, catalyzed by RNA-editing enzymes known as adenosine deaminases acting on RNA (ADARs). The traditional nuclear import pathway for ADARs involves the recognition of a putative classical nuclear localization sequence (NLS) by importin α4 and α5. In our previous research, ADAR in silkworm, <em>Bombyx mori</em> (BmADARa) was confirmed predominantly located in the nucleus. However, the location of the NLS in BmADARa and its impact on nuclear import and self-dimerization remained unclear. Utilizing NLS prediction software, we predicted the presence of a bipartite NLS within the amino-terminal, 85 amino acids of BmADARa (N85). This prediction was validated through point mutation, which demonstrated that the bipartite NLS could directly mediate nuclear import of BmADARa. Co-immunoprecipitation analysis revealed that BmADARa is mainly dependent on BmKaryopherin α3 (homologous to mammalian importin α4) for nuclear import, although both BmKaryopherin α3 and BmImportin α5 could recognize bipartite NLS. The N-terminal truncated mutants and the bipartite NLS mutants of BmADARa suggest that the bipartite NLS is the major nuclear import site and a crucial structure for self-dimerization of BmADARa. In conclusion, the N-terminal bipartite NLS of BmADARa is recognized by BmKaryopherin α3 and BmImportin α5, facilitating its nuclear import. This promotes BmADARa self-dimerization and maintains the stability of dimerization, thereby enhancing its editing efficiency on target substrates. The results of this research demonstrate the role of bipartite NLS in BmADARa editing and laying a foundation for further research on the regulation of BmADARa in the growth and development in <em>B. mori</em>.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104190"},"PeriodicalIF":3.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing: A novel approach to manage insect vectors of plant viruses","authors":"Sumit Jangra ,&nbsp;Jesse Potts ,&nbsp;Amalendu Ghosh ,&nbsp;Dakshina R. Seal","doi":"10.1016/j.ibmb.2024.104189","DOIUrl":"10.1016/j.ibmb.2024.104189","url":null,"abstract":"<div><div>Insect vectors significantly threaten global agriculture by transmitting numerous plant viruses. Various measures, from conventional insecticides to genetic engineering, are used to mitigate this threat. However, none provide complete resistance. Therefore, researchers are looking for novel control options. In recent years with the advancements in genomic technologies, genomes and transcriptomes of various insect vectors have been generated. However, the lack of knowledge about gene functions hinders the development of novel strategies to restrict virus spread. RNA interference (RNAi) is widely used to elucidate gene functions, but its variable efficacy hampers its use in managing insect vectors and plant viruses. Genome editing has the potential to overcome these challenges and has been extensively used in various insect pest species. This review summarizes the progress and potential of genome editing in plant virus vectors and its application as a functional genomic tool to elucidate virus-vector interactions. We also discuss the major challenges associated with editing genes of interest in insect vectors.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104189"},"PeriodicalIF":3.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of OR5, which is highly expressed in the winged grain aphid Sitobion miscanthi, in specific recognition of EBF","authors":"Jia Fan ,&nbsp;Bing Wang ,&nbsp;Tianjiao Zhang ,&nbsp;Miaomiao Yu ,&nbsp;Mengyu Wang ,&nbsp;Siyu Zhang ,&nbsp;Changqing Su ,&nbsp;Jingyang Xu ,&nbsp;Xin Jiang ,&nbsp;Qian Zhang ,&nbsp;Guirong Wang ,&nbsp;Julian Chen","doi":"10.1016/j.ibmb.2024.104180","DOIUrl":"10.1016/j.ibmb.2024.104180","url":null,"abstract":"<div><p>Winged parthenogenetic aphids are mainly responsible for migration and dispersal. Aphid alarm pheromone (<em>E</em>)-β-Farnesene (EBF) has dual effects on repelling and stimulating wing differentiation in aphids. Previous studies have shown that the odorant coreceptor SmisOrco is involved in the perception of EBF by <em>S. miscanthi</em>; however, its EBF-specific odorant receptor (OR) and the difference between winged and wingless aphids remain unclear. In this study, the <em>Xenopus oocyte</em> expression system and RNAi technology were used to detect the transmission of EBF signals, and it was found that the olfactory receptor <em>SmisOR5</em> is an EBF-specific OR in <em>S. miscanthi</em> and is specifically highly expressed in the antennae of winged aphids. Furthermore, when <em>OR5</em> was silenced with dsRNA, the repellent effect of EBF was weakened, and aphids showed more active aimless movements. Therefore, as a specific OR for EBF, the high expression level of <em>SmisOR5</em> in winged aphids suggests a molecular basis for its high sensitivity to EBF. This study advances our understanding of the molecular mechanisms of aphid EBF perception and provides novel ideas for effective management and prevention of the migration of winged aphids.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"173 ","pages":"Article 104180"},"PeriodicalIF":3.2,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipogenesis in Nasonia vitripennis: Influence of sugar chemistry, preferential production of triacylglycerides, and comparison of fatty acid biosynthetic capacity with Drosophila melanogaster","authors":"Joachim Ruther,&nbsp;Julian Hoheneder,&nbsp;Vera Koschany","doi":"10.1016/j.ibmb.2024.104179","DOIUrl":"10.1016/j.ibmb.2024.104179","url":null,"abstract":"<div><p>Sugar consumption increases the fecundity and longevity in many species of parasitic wasps (parasitoids) but whether these insects use sugars to synthesize significant amounts of fatty acids and storage fat de novo (lipogenesis) is discussed controversially. It has long been assumed that parasitic wasps lost this ability during evolution, mainly because in several species wasps with ad libitum access to sugar did not increase teneral lipid levels. Recent studies demonstrated that many species are nonetheless capable of synthesizing fatty acids de novo from glucose. It is unclear, however, whether also other sugars are used for fatty acid biosynthesis and whether an increase of sugar concentration to levels occurring in natural sugar sources translates into higher fatty acid production. Furthermore, it has been suggested that fatty acid production in parasitoids is negligible compared to species increasing teneral fat reserves such as <em>Drosophila melanogaster.</em> Here we show by stable isotope labeling experiments that females of <em>Nasonia vitripennis</em> convert D-glucose, D-fructose, sucrose, and α,α-trehalose, major sugars consumed by adult parasitoids in nature, equally well to palmitic, stearic, oleic, and linoleic acid. Lipogenesis from D-galactose occurs as well albeit to a lesser extent. Sugar concentration is crucial for lipogenic activity, and almost 80% of de novo synthesized fatty acids were incorporated into storage fat (triacylglycerides). Comparison of fatty acid biosynthesis within a 48-h feeding period with <em>D. melanogaster</em> revealed that <em>N. vitripennis</em> produced approximately half as many fatty acids per body mass unit. Both species fed equal amounts of the glucose offered. We conclude that lipogenesis is far from negligible in <em>N. vitripennis</em> and plays an important role for the energy balance when teneral lipid reserves deplete.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"173 ","pages":"Article 104179"},"PeriodicalIF":3.2,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0965174824001103/pdfft?md5=d910e65536a49cc9d870ed05ed81e8d5&pid=1-s2.0-S0965174824001103-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}