Insect Biochemistry and Molecular Biology最新文献_第2页

Research progress on the biological characteristics and the synthesis pathway of cantharidin in the family meloidae (Insecta: Coleoptera) 花蛾科斑蝥素生物学特性及合成途径的研究进展。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-06-13 DOI: 10.1016/j.ibmb.2025.104345

Qian Sun , Bing Wang , Yingying Xie , Fei Xue , Shuangcheng Ma , Bonian Zhao , Yongqiang Lin

{"title":"Research progress on the biological characteristics and the synthesis pathway of cantharidin in the family meloidae (Insecta: Coleoptera)","authors":"Qian Sun , Bing Wang , Yingying Xie , Fei Xue , Shuangcheng Ma , Bonian Zhao , Yongqiang Lin","doi":"10.1016/j.ibmb.2025.104345","DOIUrl":"10.1016/j.ibmb.2025.104345","url":null,"abstract":"<div><div>Cantharidin, an active compound with well-established clinical efficacy, is a defensive secondary metabolite primarily derived from insects in the <em>Meloidae</em> family. The study of cantharidin's synthetic pathway is not only one of the most intriguing research areas within the <em>Meloidae</em> family, but also holds promise in bridging the gap between biological defense mechanisms and clinical applicability. This paper provides a concise overview of the cantharidin-related biological characteristics of <em>Meloidae</em> beetles. It then summarizes recent advances in research on the synthetic pathway of cantharidin, focusing on three key areas: the synthesis of sesquiterpene precursors, the production and metabolism of farnesol, and its connection to juvenile hormone metabolism. The hypothesis is proposed that the biosynthetic pathway starts with the mevalonate pathway, which synthesizes farnesol, the identified precursor of cantharidin. Farnesol is subsequently converted by enzymes into farnesoic acid, which is further transformed into juvenile hormone acid. Juvenile hormone acid is then converted into juvenile hormone acid diol, which participates in the synthesis of cantharidin. Finally, we propose a speculative synthesis pathway for cantharidin based on current research findings and discuss its implications.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104345"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionotropic receptors participate in the perception of sinigrin in Plutella xylostella adults 嗜电性受体参与小菜蛾成虫对紫红素的感知

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-06-07 DOI: 10.1016/j.ibmb.2025.104344

Zhi-Qiang Wei , Ya-Xin Wang , Ji-Xiang Wang , Xuan-Pu Luan , Chen Ding , Fan-Zheng Meng , Yu He , Qi Yan , Jin Zhang , Shuang-Lin Dong

{"title":"Ionotropic receptors participate in the perception of sinigrin in Plutella xylostella adults","authors":"Zhi-Qiang Wei , Ya-Xin Wang , Ji-Xiang Wang , Xuan-Pu Luan , Chen Ding , Fan-Zheng Meng , Yu He , Qi Yan , Jin Zhang , Shuang-Lin Dong","doi":"10.1016/j.ibmb.2025.104344","DOIUrl":"10.1016/j.ibmb.2025.104344","url":null,"abstract":"<div><div>Glucosinolates serve as the key gustatory cue for crucifer specialists to select their host plants, however, the molecular mechanisms underlying the gustation remain poorly understood. Here, we demonstrated that two ionotropic receptor co-receptors (Ircos) were involved in the perception of a major glucosinolate, sinigrin, in the adult <em>Plutella xylostella</em>. First, we cloned 13 gustatory receptors (GRs) expressed in adults (antennae and tarsi) and larvae (heads), and analyzed their functions using the <em>Xenopus</em> oocyte expression system. It showed that none of these GRs responded to sinigrin. Then we turned to ionotropic receptors (IRs), particularly the two Ircos (PxylIR25a and PxylIR76b), starting with establishment of two single Irco knockout and one double Ircos knockout insect strains using CRISPR-Cas9 approach. Further behavioral assays revealed that knockout of these two Ircos (in both single and double knockout) significantly reduced the sinigrin perception in female adults with no difference among the three knockout strains, suggesting that the two IRs participate in collaboration in the sinigrin perception. However, larval feeding preference for the sinigrin remained generally unaffected in the three knockout strains. These results demonstrate that adult <em>P. xylostella</em> perceive sinigrin at least partly through IR-mediated pathway, but the larvae employ other pathways. Our findings provide new insights into the gustatory mechanisms of glucosinolates, and provide potential molecular targets for development of new control strategies in <em>P</em>. <em>xylostella</em> and other crucifer specialists.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104344"},"PeriodicalIF":3.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic and transcriptomic analysis of the effects of SRBSDV infection on saliva secretion in WBPH SRBSDV感染对WBPH唾液分泌影响的蛋白质组学和转录组学分析。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-06-06 DOI: 10.1016/j.ibmb.2025.104343

Qing-Ling Qi, Xiao-Han Xu, Shiqiang Xu, Jian-Ping Chen, ZongTao Sun, Wei Wu

{"title":"Proteomic and transcriptomic analysis of the effects of SRBSDV infection on saliva secretion in WBPH","authors":"Qing-Ling Qi, Xiao-Han Xu, Shiqiang Xu, Jian-Ping Chen, ZongTao Sun, Wei Wu","doi":"10.1016/j.ibmb.2025.104343","DOIUrl":"10.1016/j.ibmb.2025.104343","url":null,"abstract":"<div><div>Most plant viruses rely on insect vectors for transmission, with insect saliva serving as a critical interface in tripartite virus-vector-host interactions. Southern rice black-streaked dwarf virus (SRBSDV), a destructive pathogen transmitted by the white-backed planthopper (<em>Sogatella furcifera</em>, WBPH), severely threatens rice production, yet the mechanisms by which SRBSDV manipulates WBPH salivary secretion to enhance viral spread remain poorly understood. Here, integrated proteomic and transcriptomic analyses revealed that SRBSDV infection significantly reduces salivary protein diversity and abundance in WBPH, including digestive enzymes, redox regulators, and effector proteins critical for suppressing plant defenses. LC-MS/MS-based quantification demonstrated a marked decline in secreted protein types and altered abundance profiles in SRBSDV-infected WBPH compared to SRBSDV-free counterparts. Transcriptomic profiling identified 1803 differentially expressed genes in SRBSDV-infected salivary glands, with transmembrane transport-related pathways being prominently downregulated. Crucially, cross-omics integration revealed minimal correlation between salivary protein secretion and transcriptional changes, suggesting that SRBSDV primarily disrupts salivary function by impairing transmembrane transport capacity rather than directly suppressing protein synthesis. This secretory defect likely attenuates the insect's ability to neutralize plant defenses, driving behavioral shifts toward frequent probing that enhance viral inoculation efficiency. Our study unveils a novel strategy whereby SRBSDV hijacks vector secretory machinery to optimize transmission, advancing understanding of virus-vector coevolution and providing a foundation for effector-targeted interventions to block viral spread.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104343"},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a cytochrome P450, CYP6CY6v2, as a cytokinin side-chain hydroxylase from the aphid Acyrthosiphon pisum 蚜虫细胞分裂素侧链羟化酶CYP6CY6v2的鉴定

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-06-02 DOI: 10.1016/j.ibmb.2025.104333

Kei Sato , Zui Fujimoto , Yoshihito Suzuki

引用次数: 0

The cys-loop ligand-gated ion channel gene superfamilies of the Lepidopteran species, Bombyx mori and Chilo suppressalis 鳞翅目、家蚕和家蚕的cys环配体门控离子通道基因超家族。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-30 DOI: 10.1016/j.ibmb.2025.104331

Andrew K. Jones , Zijiao Song , Qiutang Huang , Xin Mao , Zhongqiang Jia , Jie Jiang , Tejeswini Dandu , Chunqing Zhao

{"title":"The cys-loop ligand-gated ion channel gene superfamilies of the Lepidopteran species, Bombyx mori and Chilo suppressalis","authors":"Andrew K. Jones , Zijiao Song , Qiutang Huang , Xin Mao , Zhongqiang Jia , Jie Jiang , Tejeswini Dandu , Chunqing Zhao","doi":"10.1016/j.ibmb.2025.104331","DOIUrl":"10.1016/j.ibmb.2025.104331","url":null,"abstract":"<div><div>The rice stem borer, <em>Chilo suppressalis</em> (Walker), is a major pest of rice crops. It is commonly managed by pesticides, several of which act on cys-loop ligand-gated ion channels (cysLGICs). In insects, the cysLGIC superfamily consists of receptors that mediate fast excitatory or inhibitory synaptic transmission in the nervous system as well as performing non-neuronal roles. We present here the cysLGIC superfamily of <em>C. suppressalis</em> as well as that of the silk worm, <em>Bombyx mori</em> (Linnaeus), the first complete gene families from Lepidopteran species, which consists of 27 and 24 subunit-encoding genes, respectively. As with other insects, <em>Chilo</em> and <em>Bombyx</em> possess ion channels predicted to be gated by acetylcholine, γ-aminobutyric acid, glutamate and histamine, as well as orthologues of the <em>Drosophila</em> pH-sensitive chloride channel, CG8916 and CG12344. Both <em>C. suppressalis</em> and <em>B. mori</em> lack GRD (GABA<sub>A</sub> and glycine receptor-like subunit of <em>Drosophila</em>), instead possessing extra RDL (resistance to dieldrin) subunits. Quantitative polymerase chain reaction analysis showed that the <em>C. suppressalis</em> cysLGIC subunits can show differential expression levels throughout the insect's life cycle. Our findings reveal the complete cysLGIC gene families of <em>B. mori</em> and <em>C. suppressalis</em>. These findings enhance our understanding of the evolution of the insect cysLGIC superfamily and provide a useful basis for studying their function and action of pesticides.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104331"},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A testis-specific heat shock protein gene plays a critical role in male fertility in the fall armyworm 睾丸特异性热休克蛋白基因在秋粘虫雄性生殖能力中起关键作用

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-28 DOI: 10.1016/j.ibmb.2025.104332

Yuchen Zhao , Pei Wang , Yalan Cheng , Siyu Tian , Junfeng Zuo , Qianguang Fang , Chenxue Ling , Subba Reddy Palli , Xien Chen

{"title":"A testis-specific heat shock protein gene plays a critical role in male fertility in the fall armyworm","authors":"Yuchen Zhao , Pei Wang , Yalan Cheng , Siyu Tian , Junfeng Zuo , Qianguang Fang , Chenxue Ling , Subba Reddy Palli , Xien Chen","doi":"10.1016/j.ibmb.2025.104332","DOIUrl":"10.1016/j.ibmb.2025.104332","url":null,"abstract":"<div><div>Heat shock proteins (Hsps) are critical for stress responses and multiple physiological processes, yet their function in insect reproduction remains underexplored. In this study, we identified 36 Hsp genes in the fall armyworm (<em>Spodoptera frugiperda</em>), of which LOC118269601 (designated as <em>SfHsp68A</em>) exhibited robust testis-specific expression, peaking during pupal testis development. CRISPR/Cas9-mediated knockout of <em>SfHsp68A</em> resulted in recessive male sterility, significantly reduced testis size, sperm counts, and egg hatch rates. Transcriptomic analysis of mutant testes revealed substantial downregulation of genes linked to mitochondrial function, oxidative phosphorylation, energy metabolism, and reactive oxygen species (ROS) metabolism. These results demonstrate that SfHsp68A is essential for male fertility in <em>S. frugiperda,</em> likely by maintaining mitochondrial integrity and metabolic homeostasis during spermatogenesis. These studies reveal that testis-specific Hsps function in male fertility and highlight <em>SfHsp68A</em> as a potential genetic target for developing sterile insect technology, offering an environmentally sustainable strategy for controlling this globally invasive pest.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104332"},"PeriodicalIF":3.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino acid and acylcarnitine profile of Aedes aegypti (L) post-blood feeding, after permethrin exposure, and during the life cycle 埃及伊蚊吸血后、氯菊酯暴露后和生命周期内氨基酸和酰基肉碱谱。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-22 DOI: 10.1016/j.ibmb.2025.104328

Mariana Lizbeth Jiménez-Martínez , Gerardo de Jesús Trujillo-Rodriguez , María de Lourdes Ramírez-Ahuja , Olga Karina Villanueva-Segura , Patricio Adrián Zapata-Morin , Griffith Samuel Lizarraga , Laura E. Martinez-de-Villarreal , Margarita L. Martinez-Fierro , Ivan Delgado-Enciso , Jose Ignacio Gonzalez-Rojas , Adriana E. Flores , Antonio Guzman-Velasco , Iram Pablo Rodriguez-Sanchez

{"title":"Amino acid and acylcarnitine profile of Aedes aegypti (L) post-blood feeding, after permethrin exposure, and during the life cycle","authors":"Mariana Lizbeth Jiménez-Martínez , Gerardo de Jesús Trujillo-Rodriguez , María de Lourdes Ramírez-Ahuja , Olga Karina Villanueva-Segura , Patricio Adrián Zapata-Morin , Griffith Samuel Lizarraga , Laura E. Martinez-de-Villarreal , Margarita L. Martinez-Fierro , Ivan Delgado-Enciso , Jose Ignacio Gonzalez-Rojas , Adriana E. Flores , Antonio Guzman-Velasco , Iram Pablo Rodriguez-Sanchez","doi":"10.1016/j.ibmb.2025.104328","DOIUrl":"10.1016/j.ibmb.2025.104328","url":null,"abstract":"<div><div><em>Aedes aegypti</em>, a major vector for arboviruses, requires effective control strategies due to rising insecticide resistance. This study quantified 12 amino acids and 24 acylcarnitines in two strains of <em>Ae. aegypti</em> (New Orleans, susceptible; Apodaca, resistant) using tandem mass spectrometry (MS/MS) across three approaches: 1) life cycle stages, 2) fasting and post-blood feeding (24, 48, 72 h), and 3) before and after permethrin exposure. In the life cycle, alanine (Ala) was highest in adult males (40.3 %), arginine (Arg) peaked in adult females (18.35 %), and tyrosine (Tyr) reached 14.74 % in larvae. The acylcarnitine C0 (free carnitine) peaked in larvae stage 4 (94.21 %), while C2 (acetyl carnitine) was highest in adult females (47.68 %). Post-blood feeding, Ala peaked at 72 h (43.34 %), proline (Pro) at 48 h (20.63 %), and Arg decreased from 0 h (18.35 %) to 72 h (11.15 %). Acylcarnitines C0 and C2 showed a decline post-feeding, indicating fatty acid mobilization. After permethrin exposure, resistant mosquitoes exhibited higher Ala (51.7 %) and C2 (57.9 %) levels, suggesting increased metabolic activity for detoxification. In contrast, susceptible mosquitoes displayed less variability in amino acid and acylcarnitine levels. These results provide valuable insights into the metabolic adaptations of <em>Ae. aegypti</em>, indicating the potential for targeting amino acid and acylcarnitine pathways to improve vector control strategies, particularly in the context of rising insecticide resistance.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104328"},"PeriodicalIF":3.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of biliverdin as a pigment involved in the seasonal variation of green lacewing body colour and the enzymes involved in its metabolism 绿草体色季节变化中胆绿素及其代谢酶的鉴定。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-20 DOI: 10.1016/j.ibmb.2025.104330

Shion Abe, Yuka Matsubara, Kanako Ogawa, Yuki Nakamura, Satoshi Yamauchi, Hisashi Nishiwaki

{"title":"Identification of biliverdin as a pigment involved in the seasonal variation of green lacewing body colour and the enzymes involved in its metabolism","authors":"Shion Abe, Yuka Matsubara, Kanako Ogawa, Yuki Nakamura, Satoshi Yamauchi, Hisashi Nishiwaki","doi":"10.1016/j.ibmb.2025.104330","DOIUrl":"10.1016/j.ibmb.2025.104330","url":null,"abstract":"<div><div>The green lacewing, <em>Chrysoperla nipponensis</em>, is an insect that undergoes complete metamorphosis, and its body colour changes depending on the developmental stage; adults and larvae are greenish and brownish, respectively. In addition, the body colour of some adults changes to brown in winter, suggesting that environmental factors influence pigment biosynthesis in this insect. However, the underlying colour regulation systems remain unknown. In this study, we purified the pigments that produce the body colour of green adult lacewings and identified biliverdin via MS/MS analyses. We therefore elucidated the mRNA and amino acid sequences of biliverdin-related biosynthetic enzymes, such as haem oxygenase (HO) and biliverdin reductase (BR), in <em>C. nipponensis</em>. By expressing recombinant BR in <em>E. coli</em>, we investigated the ability of BR to metabolize biliverdin. In addition, via RNAi techniques, we examined whether the suppression of these enzymes changed body colour. Finally, we quantified the expression levels of the mRNAs encoding these enzymes and compared those in green adults reared under normal conditions with those in brown adults reared under winter-mimicking conditions. The results suggest that not only biliverdin but also other green pigments contribute to the green body colour of adult lacewings.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104330"},"PeriodicalIF":3.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas9-induced point mutation on the GABA receptor subunit RDL confers high resistance to phenylpyrazole insecticides in the rice planthopper Laodelphax striatellus CRISPR/ cas9诱导的GABA受体亚基RDL点突变使稻褐飞虱对苯吡唑类杀虫剂具有高抗性

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-20 DOI: 10.1016/j.ibmb.2025.104327

Hui-Jie Wu , Jia-Peng Yang , Wen-Jing Ma , Zheng-Hao Li , Hong-Yu Feng , Zhang-Nv Yang , Hai-Jun Xu

{"title":"A CRISPR/Cas9-induced point mutation on the GABA receptor subunit RDL confers high resistance to phenylpyrazole insecticides in the rice planthopper Laodelphax striatellus","authors":"Hui-Jie Wu , Jia-Peng Yang , Wen-Jing Ma , Zheng-Hao Li , Hong-Yu Feng , Zhang-Nv Yang , Hai-Jun Xu","doi":"10.1016/j.ibmb.2025.104327","DOIUrl":"10.1016/j.ibmb.2025.104327","url":null,"abstract":"<div><div>The Delphacidae planthopper <em>Laodelphax striatellus</em> (Order: Hemiptera) is one of the most damaging insect pests of rice crops in Asia. The phenylpyrazole insecticide fipronil was introduced in China in the mid-1990s to control these pests, but its widespread use has led to the development of high levels of resistance. Field sampling coupled with <em>in vitro</em> assays indicated that an A2′N-point mutation in the gamma-aminobutyric acid receptor RDL has been linked to fipronil resistance; however, genetic evidence supporting this association has been lacking. Here, we generated an A2′N-point mutation (<em>LsRDL</em><sup><em>A/N</em></sup>) in <em>L. striatellus</em> using CRISPR/Cas9-mediated homologous recombination. Bioassay revealed that the <em>LsRDL</em><sup><em>A/N</em></sup> mutants conferred a 1211-fold higher resistance to fipronil compared to wild-type planthoppers. Furthermore, these mutants also showed significant resistance (>1000-fold) to ethiprole but not to imidacloprid, demonstrating that the A2′N mutation confers resistance specific to phenylpyrazole insecticides. However, the <em>LsRDL</em><sup><em>A/N</em></sup> mutants displayed reduced fecundity, lower egg hatching rates, and prolonged egg and nymph stages, indicating that fipronil resistance carries a substantial fitness cost. These findings enhance our understanding of the genetic mechanisms underlying phenylpyrazole resistance and provide valuable insights for developing effective resistance management strategies for planthoppers.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104327"},"PeriodicalIF":3.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silkworm mutagenesis using a ribonucleoprotein-based CRISPR/Cas12a system 利用基于核糖核蛋白的CRISPR/Cas12a系统进行家蚕诱变

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-19 DOI: 10.1016/j.ibmb.2025.104329

Yunlong Zou , Aijun Ye , Meixin Dong, Yuhao Zhou, Wentao Wu, Yuxia Tang, Hai Hu, Fangyin Dai, Xiaoling Tong

{"title":"Silkworm mutagenesis using a ribonucleoprotein-based CRISPR/Cas12a system","authors":"Yunlong Zou , Aijun Ye , Meixin Dong, Yuhao Zhou, Wentao Wu, Yuxia Tang, Hai Hu, Fangyin Dai, Xiaoling Tong","doi":"10.1016/j.ibmb.2025.104329","DOIUrl":"10.1016/j.ibmb.2025.104329","url":null,"abstract":"<div><div>The development of highly efficient genome editing tools has revolutionized developmental biology and genetic studies in silkworm. Although methods based on CRISPR/Cas9 are currently popular, the Cas12a system has emerged as a promising option. However, it has not yet been applied to target the silkworm genome <em>in vivo,</em> and its activity in silkworm has not yet been characterized. In this study, we established a ribonucleoprotein-based CRISPR/Cas12a system, and compared it to the CRISPR/Cas9 system using 19 crRNA and 17 sgRNAs to target three different genes <em>in vivo.</em> Although Cas12a generates mutants less efficiently than Cas9, we used it successfully to generate transmissible indels, and demonstrated its application by targeting the <em>FibH</em> and <em>mp</em> genes to produce mutants with the expected phenotypes. We also assessed the influence of temperature (37 °C vs. 25 °C) on Cas12a activity, and demonstrated that the effects are target dependent. In summary, we have established a ribonucleoprotein-based CRISPR/Cas12a system in silkworm that offers a practical alternative to CRISPR/Cas9 and extends the genome editing tool box available for silkworm research.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104329"},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0