Journal of Dairy Science最新文献

{"title":"INTERPRETIVE SUMMARIES, DECEMBER 2024","authors":"","doi":"10.1016/S0022-0302(24)01324-9","DOIUrl":"10.1016/S0022-0302(24)01324-9","url":null,"abstract":"","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":"107 12","pages":"Pages xii-xxvii"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor: Comment on the publication of Zened et al. (2024)","authors":"Anton Vorndran, Julia Steinhoff-Wagner","doi":"10.3168/jds.2024-24925","DOIUrl":"10.3168/jds.2024-24925","url":null,"abstract":"","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":"107 12","pages":"Pages 10047-10048"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor: Response to comment on Zened et al. (2024)","authors":"Asma Zened","doi":"10.3168/jds.2024-25029","DOIUrl":"10.3168/jds.2024-25029","url":null,"abstract":"","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":"107 12","pages":"Page 10049"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rumen-protected methionine supplementation improves lactation performance and alleviates inflammation during a subclinical mastitis challenge in lactating dairy cows","authors":"A. Paz ,&nbsp;T.C. Michelotti ,&nbsp;M. Suazo ,&nbsp;J. Bonilla ,&nbsp;M. Bulnes ,&nbsp;A. Minuti ,&nbsp;D. Luchini ,&nbsp;E. Trevisi ,&nbsp;A.F. Lima ,&nbsp;J. Halfen ,&nbsp;M. Rovai ,&nbsp;J.S. Osorio","doi":"10.1016/j.jods.2024.08.001","DOIUrl":"10.1016/j.jods.2024.08.001","url":null,"abstract":"<div><div>This study aimed to evaluate the effects of rumen-protected Met on lactation performance, inflammation, and immune response, and liver glutathione of lactating dairy cows during a subclinical mastitis challenge (SMC). Thirty-two Holstein cows (145 ± 51 DIM) were enrolled in a randomized complete block design. At −21 d relative to the SMC, cows were assigned to dietary treatments, and data were collected before and during the SMC. Cows were blocked according to parity, DIM, and milk yield and received a basal diet (17.4% CP; Lys 7.01% MP and Met 2.14% MP) plus 100 g/d of ground corn (CON; n = 16) or a basal diet plus 100 g/d of ground corn and rumen-protected Met (SM, Smartamine M at 0.09% of dietary DM; n = 16), fed as a top-dress. At 0 d, the mammary gland's rear right quarter was infused with 100,000 cfu of <em>Streptococcus uberis</em> (O140J). Milk yield was recorded twice daily from 0 until 3 d relative to SMC. Milk samples were collected during each milking from 0 to 3 d relative to SMC, blood samples were collected at 0, 6, 12, 24, 48, and 72 h relative to SMC. The mTOR pathway activation was assessed in immune cells in blood and milk samples by measuring quantity and phosphorylation status of mTOR-related proteins, including AKT, S6RP, and 4EBP1. For the ratio of phosphorylated to total AKT, S6RP, and 4EBP1, blood samples were collected at 0, 12, and 24 h, and milk samples at 24 h relative to SMC. Liver biopsies were performed at −10 d and 24 h relative to SMC for measurement of glutathione. Linear mixed models with repeated measures were used to analyze the results. There was a trend for greater milk yield per milking (+ 0.8 kg) and per day (+1.7 kg) after SMC in SM cows compared with CON. The DMI was not affected by dietary treatments. Reactive oxygen metabolites were lower in SM cows than in CON. Milk somatic cell linear score was not affected by dietary treatments, and a score &gt;4 at 24 h confirmed subclinical mastitis. The SM cows had greater milk fat percentage at 24 and 36 h post SMC, resulting in overall greater milk fat. Milk protein tended to be greater in SM cows than in CON. We observed greater liver glutathione in SM cows than in CON. Among inflammation biomarkers, ceruloplasmin was lower for SM cows compared with CON. In milk, greater phosphorylated (p) AKT:AKT and pS6RP:S6RP ratios were observed in immune cell populations from SM cows compared with CON. Blood neutrophils had a greater p4EBP1:4EBP1 ratio in SM cows compared with CON. Overall, our results show that Met supplementation during an SMC positively affected milk performance, lowered the risk of oxidative stress, and attenuated inflammation partially by increasing liver glutathione and immune cells' protein synthesis via mTOR signaling.</div></div>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":"107 12","pages":"Pages 10761-10775"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter from the Editor: Some pointers on figure preparations","authors":"Paul J. Kononoff Editor in Chief","doi":"10.3168/jds.2024-25856","DOIUrl":"10.3168/jds.2024-25856","url":null,"abstract":"","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":"107 12","pages":"Pages 10043-10046"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of calcium and buffer sources on lactational performance, ruminal fermentation, nutrient digestibility, and metabolism of dairy cows","authors":"L.F. Martins ,&nbsp;K.C. Welter ,&nbsp;D.E. Wasson ,&nbsp;S.F. Cueva ,&nbsp;N. Stepanchenko ,&nbsp;J.S. Dowd ,&nbsp;J.R. Hart ,&nbsp;A.N. Hristov","doi":"10.3168/jds.2024-25089","DOIUrl":"10.3168/jds.2024-25089","url":null,"abstract":"<div><div>The objective was to investigate the effects of calcium and rumen buffer sources on lactational performance, ruminal fermentation, enteric gas emissions, apparent total-tract digestibility of nutrients, and blood variables of lactating dairy cows. A replicated 3 × 3 Latin square design experiment was conducted with 9 primi- and 9 multiparous mid-lactation Holstein cows. Cows were fed the same basal diet, except for the inclusion (as % DMI) of the following minerals: (1) CON, 0.80% limestone and 0.55% NaCl; (2) BICARB, 0.80% limestone and 0.80% NaHCO₃; and (3) ARAG, 0.80% aragonite and 0.55% NaCl. Dry matter intake was decreased by ARAG compared with CON but not with BICARB. Treatments did not affect milk yield, ECM, or yields of milk components. Feed efficiency was increased by BICARB compared with CON but not with ARAG (i.e., 1.72, 1.64, and 1.70, respectively). Compared with CON, milk fat was increased by both BICARB and ARAG (3.32% vs. 3.58%, respectively). Milk protein concentration tended to be slightly decreased (i.e., 2%) by ARAG compared with CON but not with BICARB. Milk total solids and MUN concentrations were increased by BICARB and tended to be increased by ARAG, compared with CON. Treatments did not affect ruminal fermentation variables, except that ARAG increased butyrate molar proportion, compared with CON but not with BICARB. Additionally, ruminal NH₃ concentration was greater for ARAG than for BICARB. Enteric gas emission and apparent total-tract digestibility of nutrients were not affected by treatments in the current study. Blood pH was not affected by treatments, and blood ionized Ca concentration was greater for ARAG than for BICARB, but neither was different from CON. Treatments did not affect plasma haptoglobin, β-hydroxybutyrate, or urea N concentrations. Overall, rumen buffering capacity of ARAG appears to be similar to that of BICARB, which was supported by increased milk fat, compared with CON. Compared with BICARB, ARAG increased ionized Ca concentration because of decreased base excess and HCO₃⁻ concentrations in blood.</div></div>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":"107 12","pages":"Pages 10680-10694"},"PeriodicalIF":3.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in antibiofilm strategies in the dairy industry.","authors":"Coralie Goetz, Laurie Sanschagrin, Eric Jubinville, Mario Jacques, Julie Jean","doi":"10.3168/jds.2024-25554","DOIUrl":"https://doi.org/10.3168/jds.2024-25554","url":null,"abstract":"<p><p>Biofilm formation allows microorganisms including bacteria to persist on abiotic or biotic surfaces, to resist treatments with biocides (disinfectants and antibiotics) and to evade the immune response in animal hosts much more than they do in the planktonic form. Bacteria able to form biofilm can be troublesome in the dairy industry, both by causing clinical symptoms in livestock and by colonizing milking devices and milk processing equipment, resulting in dairy products of lower quality and sometimes raising serious food safety issues. In fact, most of the bacterial species isolated frequently in the dairy chain have the ability to form biofilm. Common examples include Staphylococcus aureus and other staphylococci that frequently infect mammary glands, but also Bacillus spp., Listeria monocytogenes and Pseudomonas spp. which cause spoilage of dairy products and sometimes foodborne illnesses. The economic losses due to biofilm formation in the dairy industry are considerable, and scientists are constantly solicited to develop new antibiofilm strategies, especially using biocides of natural origin. Although the number of studies in this subject area has exploded in recent years, the in vivo efficacy of most novel approaches remains to be explored. Used alone or to increase the efficacy of disinfectants or antibiotics, they could allow the implementation of strategies having less impact on the environment. Their use is expected to lead to less reliance on antibiotics to treat intramammary infections in dairy farms and to the use of lower concentrations of chemical disinfectants in dairy processing plants.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic hydrolysis of buffalo casein enhances DPP-4 inhibition: structural modifications and bioactive peptide identification.","authors":"Ning An, Jing Yang, Yu Zhang, Huayi Suo, Jiajia Song","doi":"10.3168/jds.2024-25552","DOIUrl":"https://doi.org/10.3168/jds.2024-25552","url":null,"abstract":"<p><p>Dipeptidyl peptidase-4 (DPP-4), the enzyme responsible for the rapid degradation of incretin hormones, plays a pivotal role in blood glucose regulation, and its inhibition serves as an effective strategy for maintaining glucose homeostasis. The aim of this study was to investigate the effect of enzymatic hydrolysis on the structure of buffalo casein and its DPP-4 inhibitory activity. Results demonstrated that Flavorzyme effectively hydrolyzed buffalo casein, as evidenced by scanning electron microscopy and electrophoretic analysis, with the degree of hydrolysis reaching its maximum value (20.05 ± 0.14%) after 3 h. The results of circular dichroism spectra, as well as endogenous and exogenous fluorescence spectra, indicated significant alterations in the secondary and tertiary structures of buffalo casein following enzymatic hydrolysis. Additionally, the DPP-4 inhibitory effect of buffalo casein was found to increase with longer hydrolysis times. The hydrolysate obtained after 3 h of hydrolysis demonstrated the highest level of inhibition, with a half-maximal inhibitory concentration (IC₅₀) value of 1.04 mg/mL. The DPP-4 inhibitory peptide YPFPGPIPN, with an IC₅₀ value of 0.88 mg/mL, was identified in the 1-3 kDa fraction of the 3-h hydrolysate. This peptide interacted with the active site of DPP-4 via hydrogen bonds, hydrophobic interactions, salt bridges, and π-cation interactions. This study offers a novel scientific foundation for the development of functional antidiabetic foods derived from buffalo casein.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing somatic cell count and leukocyte composition in cow milk: a field study.","authors":"S Mondini, G Gislon, M Zucali, A Sandrucci, A Tamburini, L Bava","doi":"10.3168/jds.2024-25357","DOIUrl":"https://doi.org/10.3168/jds.2024-25357","url":null,"abstract":"<p><p>In recent years, there has been a proliferation of studies investigating the composition of somatic cell count (SCC) of milk, focusing on neutrophils (NEU), lymphocytes (LYM), and macrophages (MAC). These 3 components are indeed crucial for the animal's immune response to mastitis-causing pathogens. The study examined various factors influencing somatic cell count and leucocyte components in cow milk, including lactation stage, parity, and milk electrical conductivity, using data from 179 dairy cows across 6 farms throughout the entire lactation. Statistical analyses, including mixed models and logistic regression, were employed to investigate the relationships between these variables and identify risk factors for high SCC levels. Results showed that factors such as parity and lactation stage were significantly associated with somatic cell composition. In particular, the highest milk NEU values (>60% of the total leucocytic fraction) and lowest MAC values (<20%) were found at the beginning and the end of lactation, that are the critical periods for udder health. High milk electrical conductivity, low milk production, number of parity, and poor hygiene scores were identified as contributing to increased SCC. Additionally, elevated percentages of NEU and LYM in milk were associated with increased risk of high SCC values, indicating potential udder health issues.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing oral versus intravenous Ca administration on alleviating markers of production, metabolism, and inflammation during an intravenous lipopolysaccharide challenge in mid-lactation dairy cows.","authors":"J Opgenorth, B M Goetz, S Rodriguez-Jimenez, A D Freestone, G J Combs, T A Flemming, J L McGill, P J Gorden, L Tikofsky, L H Baumgard","doi":"10.3168/jds.2024-24831","DOIUrl":"https://doi.org/10.3168/jds.2024-24831","url":null,"abstract":"<p><p>Animals, including dairy cows develop hypocalcemia during infection. Prior independent research suggests supplementing oral Ca, but not i.v. Ca, improves multiple health metrics after immune activation. Therefore, study objectives were to directly compare the effects of administering an oral Ca bolus vs. i.v. Ca on mineral and energetic metabolism variables and inflammatory parameters following an i.v. lipopolysaccharide (LPS) challenge. Mid-lactation cows (124 ± 43 DIM) were assigned to 1 of 4 treatments: 1) saline control (CON; 4 mL saline; n = 4), 2) LPS control (CON-LPS; 0.375 µg/kg BW; n = 6), 3) LPS with oral Ca bolus (OCa-LPS; 0.375 µg/kg BW and a 192 g bolus of Bovikalc containing 43 g Ca [71% CaCl₂ and 29% CaSO₄] supplemented at -0.5 and 6 h relative to LPS administration; n = 8), and 4) LPS with i.v. Ca (IVCa-LPS; 0.375 µg/kg BW and 500 mL Ca-gluconate, 23% [VetOne; Boise, ID]) supplemented at -0.5 and 6 h relative to LPS infusion; n = 8). During period (P) 1 (4 d), baseline data were obtained. At the initiation of P2 (5 d), LPS and Ca supplements were administered. As anticipated, CON-LPS became hypocalcemic, but OCa-LPS and IVCa-LPS had increased ionized Ca (iCa) compared with CON-LPS cows (1.11 and 1.28 vs. 0.95 ± 0.02 mmol/L, respectively). Rectal temperature increased after LPS and was additionally elevated in IVCa-LPS from 3 to 4 h (38.9 and 39.8 ± 0.1°C in CON-LPS and IVCa-LPS, respectively). Administering LPS decreased DMI and milk yield relative to CON. Circulating glucose was decreased in OCa-LPS compared with CON-LPS and IVCa-LPS during the initial hyperglycemic phase at 1 h (75.1 vs. 94.9 and 95.7 ± 3.4 mg/dL, respectively, but all LPS infused cows regardless of treatment had similar glucose concentrations thereafter, which were decreased relative to baseline during the first 12 h. Blood urea nitrogen (BUN) increased after LPS but this was attenuated in OCa-LPS compared with CON-LPS and IVCa-LPS cows (8.7 vs. 10.0 and 10.4 ± 0.3 mg/dL). Glucagon increased in OCa-LPS and IVCa-LPS compared with CON-LPS cows (459 and 472 vs. 335 ± 28 pg/mL, respectively), and insulin markedly increased over time regardless of LPS treatment. LPS substantially increased serum amyloid A, LPS-binding protein, and haptoglobin in all treatments, but OCa-LPS tended to have increased LBP concentrations relative to IVCa-LPS (10.7 vs. 8.6 ± 0.7 µg/mL, respectively). Several cytokines increased after LPS administration, but most temporal cytokine profiles did not differ by treatment. In summary, LPS administration intensely activated the immune system and both Ca delivery routes successfully ameliorated the hypocalcemia. The i.v. and oral Ca treatments had differential effects on multiple metabolism variables and appeared to mildly influence production responses to LPS.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}