Journal of Dairy Science最新文献

{"title":"Macromineral requirements for maintenance, body weight gain, and pregnancy of dairy cows.","authors":"C H P Camisa Nova, M I Marcondes, S C Valadares Filho, M M Campos, F S Machado, L H R Silva, M M D Castro, P P Rotta","doi":"10.3168/jds.2024-25694","DOIUrl":"https://doi.org/10.3168/jds.2024-25694","url":null,"abstract":"<p><p>We aimed to predict macromineral requirements for maintenance, weight gain, and pregnancy in dairy cows. In total, 62 nonlactating cows (initial BW of 522 ± 10.1 kg [mean ± SD], initial age of 5 ± 0.5 yr, and 3 lactations) were enrolled and assigned to 3 groups: pregnant (n = 44), nonpregnant (n = 12), and baseline (n = 6). Baseline cows, which were not inseminated, were harvested at the beginning of the trial to determine the initial body composition. Both pregnant and nonpregnant groups were then divided into 2 feeding treatments: ad libitum or restricted intake at 1.15% of BW (approximating maintenance). Pregnant cows were slaughtered at 140, 200, 240, and 270 d of gestation, and nonpregnant cows were slaughtered at corresponding intervals to compare mineral accretion due to pregnancy. Total-tract digestibility was measured in six 28-d periods (d 122, 150, 178, 206, 234, and 262 of gestation) by collecting DMI, feces, and urine. The net requirements of maintenance (mg/kg of empty BW) for calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), and sulfur (S) were 13.48, 8.35, 4.06, 10.07, 45.89, and 7.82, respectively. For BW gain in pregnant cows, the models for Ca, P, Mg, K, Na, and S were set as Net Ca_gain = 0.4168 × EBW^0.7115 × EBG_c; Net P_gain = 0.8441 × EBW^0.4762 × EBG_c; Net Mg_gain = 0.0492 × EBW^0.4391 × EBG_c; Net K_gain = 0.1738 × EBW^0.5169 × EBG_c; Net Na_gain = 0.0284 × EBW^0.7880 × EBG_c; Net S_gain = 0.2530 × EBW^0.7982 × EBG_c, respectively. Estimates of net requirements of pregnancy were adjusted as follows: Net Ca_preg = 0.0042e^0.02855×^GD; Net P_preg = 0.0042e^0.02855×^GD; Net Mg_preg = 0.0042e^0.02855×^GD; Net K_preg = 0.0042e^0.02855×^GD; Net Na_preg = 0.0042e^0.02855×^GD; Net S_preg = 0.0042e^0.02855×^GD. Finally, we propose an innovative method to estimate the efficiency of macromineral utilization by gestational tissues. The efficiency of use (k_preg) for each mineral was modeled as: Ca k_preg = 0.0004e^0.0263×^GD; P k_preg = 0.2974e^0.0048×^GD; Mg k_preg = 0.00006e^0.0233×^GD; K k_preg = 0.0003e^0.0234×^GD; Na k_preg = 0.0038e^0.0200×^GD; S k_preg = 0.0004e^0.0199×^GD. These results provide valuable insights into macromineral requirements in dairy cows and offer innovative approaches to evaluating nutrient efficiency during pregnancy.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of pregnancy losses within the same lactation in grazing dairy cows.","authors":"Santiago Gerardo Corva, German Ariel Dominguez, Javier Sanchez, Rodolfo Luzbel de la Sota","doi":"10.3168/jds.2024-24924","DOIUrl":"https://doi.org/10.3168/jds.2024-24924","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The study aimed to describe pregnancy losses within the same lactation in a large commercial dairy herd in Argentina. A retrospective study was completed using 25,019 lactation records from 11,263 cows with at least 1 artificial insemination-declared pregnant (AIDP) by ultrasound at 28 to 42 d post AI. Each AIDP was identified according to the corresponding parity number, which was sequentially numbered and related to a pregnancy number within the same lactation. In each lactation, the uterine health events (UTE), retention of fetal membranes, puerperal metritis, and clinical endometritis, as well as the nonuterine health events (NUTE), clinical mastitis, and clinical lameness were recorded. The health status for each lactation was categorized according to the site of inflammation, such as healthy cows, cows with UTE, cows with NUTE records, and cows with both UTE and NUTE. Pregnancy loss was defined by: (1) detection of heat with blue paint rubbed off after having been previously diagnosed pregnant and subsequently diagnosed open by ultrasound at the next herd visit 14 d later; (2) observed abortion; or (3) diagnosis open by ultrasound pregnancy diagnosis 5 mo after AI of pregnancy to reconfirm pregnancy status. The occurrence of pregnancy loss was reported for the whole study period and 21-d periods. The risk of pregnancy loss was analyzed using a Cox proportional hazards model that included parity number, AIDP, season, health status, DIM to AIDP, and daily milk production to AIDP as covariates. Herd persistence was used to assess the risk of cows leaving the herd before the next lactation due to pregnancy loss, with the last pregnancy within each lactation, parity number, and pregnancy loss as covariates. The occurrence of pregnancy loss was 22.5%; the occurrence of pregnancy loss per 21 d was 3.7%. The median day of gestation and median DIM for the first, second, and third pregnancy losses were 98, 108, 121 d and 224, 394, and 552 d, respectively. Cows with UTE diseases had a higher hazard of pregnancy loss than healthy cows (hazard ratio [HR] 1.24, 95% CI 1.13-1.36); conversely, cows in second or third parity did not have a higher hazard of pregnancy loss than first parity cows (HR 1.03, 95% CI 0.95-1.11; HR 1.00, 95% CI 0.92-1.08; respectively). Similarly, cows with 2 and 3 AIDP had a lower hazard of pregnancy loss than cows with only 1 AIDP (HR 0.90, 95% CI 0.83-0.97; HR 0.92, 95% CI 0.85-0.99, respectively). Cows with AIDP in fall and winter had a lower HR of pregnancy loss than those with AIDP in summer (HR 0.84, 95% CI 0.77-0.91; HR 0.82, 95% CI 0.75-0.89). In conclusion, health events during lactation affect pregnancy loss within the same lactation and herd persistence to the next lactation in dairy cows. The risk of pregnancy losses within a lactation may not increase with parity number and higher AIDP, but conversely, it may increase with higher milk production (HR 1.02, 95% CI 1.01-1.02; 1%/1 kg increase in test-day milk yield","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing heavy metal risks in liquid milk: Dietary exposure and carcinogenicity in China.","authors":"Yunfeng Yang, Mingjie Pan, Yingying Lin, HuaHu Xu, Suhang Wei, Chi Zhang, Sheng Lu, Bing Niu","doi":"10.3168/jds.2025-26459","DOIUrl":"https://doi.org/10.3168/jds.2025-26459","url":null,"abstract":"<p><p>China, as the world's second-largest market for dairy products, places significant importance on the safety of such products. In recent years, incidents related to the safety of dairy products have occurred frequently, posing substantial threats to public health and societal stability. The indiscriminate discharge of industrial sewage not only causes environmental pollution, but also may result in the presence of heavy metals in raw milk, thus raising concerns about food safety. This paper establishes a risk assessment model for heavy metal contamination in liquid milk, using dietary exposure assessment and simulation risk assessment. The assessment covers heavy metal contamination in soils and pastures across most provinces in China, excluding Taiwan, Tibet, Macau, and Hong Kong. Employing methods such as the pollution index, spatial cluster analysis, distribution fitting, and carcinogenic risk assessment, the study reveals that the overall pollution levels of heavy metals in the soil of Yunnan Province and Jiangxi Province are particularly severe, and the bioaccumulation effect made heavy metal pollution more pronounced in the forage samples of Yunnan Province. Furthermore, based on transfer factors, residents' daily dietary intake, and local economic levels, the study evaluates the carcinogenic risk and target total risk index of heavy metals in raw milk. The findings highlight that the risk of carcinogenicity and noncarcinogenicity is higher in the [3,6) age group compared with other age groups, underlining the urgent need for improved regulation and monitoring to protect public health, especially for vulnerable populations. The study underscores that heavy metal contamination forms a complete \"soil-feed-milk\" risk chain. It highlights the need to establish a region-specific regulatory framework, focusing on the characteristics of pollution. In additional, dietary interventions for susceptible groups, such as children, should be strengthened to mitigate the long-term health effects of environmental pollution.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of a spray-dried fat concentrate containing tributyrin and tricaproin in milk replacer enhanced increased feed intake, growth, and elicited metabolic and endocrine responses in ad libitum-fed calves.","authors":"J N Wilms, S Hendriks, T Sugino, M H Ghaffari, M A Steele, H Sauerwein, J Martín-Tereso, L N Leal","doi":"10.3168/jds.2025-26331","DOIUrl":"https://doi.org/10.3168/jds.2025-26331","url":null,"abstract":"<p><p>Fat composition of milk replacer (MR) for calves differs from that of bovine milk fat, resulting in lower levels of butyric (C4:0) and caproic acid (C6:0). These fatty acids play a critical role in the gastrointestinal and metabolic development of calves by supporting rumen epithelial growth, enhancing energy metabolism, and promoting overall gut health. The objective of this study was to evaluate the effects of inclusion of a spray-dried fat concentrate containing tributyrin (TB) and tricaproin (TC) in a high-fat MR on growth, feed intake, and metabolic profiles of ad libitum-fed calves. Forty-eight newborn Holstein calves were blocked based on arrival sequence. Within each block of 2 calves, calves were randomly assigned to a control MR (CON, n = 24) including vegetable fats from palm, coconut, and linseed fats, or to an experimental MR including the same fat blend, to which TB and TC (TRI, n = 24) was added to the same levels found in milk fat. Both MR contained 23.7% crude protein, 27.7% fat, and 35.6% lactose (DM basis) and were fed at 13.5% solids. Calves were group housed and fed ad libitum MR with automated feeders. Weaning was gradual and induced between wk 7-10, after which calves were only fed solid feeds. Starter feed, chopped straw, and water were offered ad libitum throughout the whole study period. Calves were weighed and blood was collected once weekly at 1300h. Calves fed TRI showed a ∼50% reduction in therapeutic intervention days compared with CON. In addition, calves fed TRI consumed significantly more MR and starter feed, resulting in a greater growth. Serum NEFA and plasma total cholesterol were lower, whereas the enzymatic activity of serum ALP was higher in calves fed TRI than in CON. In addition, calves fed TRI had lower serum ghrelin and higher insulin-like growth factor-I concentrations. Incorporating TB and TC is a suitable strategy to increase solid feed intake upon weaning, resulting in better growth performance in ad libitum systems, and to improve health of dairy rearing calves.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for Imbalanced Polarization of Caruncle Macrophages in Retained Placenta of Dairy Cows.","authors":"Huijing Zhang, Ming Li, Bichen Zhao, Renxu Chang, Jingyi Wang, Yue Yang, Qingnian Huang, Ben Aernouts, Qianming Jiang, Juan J Loor, Chuang Xu","doi":"10.3168/jds.2024-26144","DOIUrl":"https://doi.org/10.3168/jds.2024-26144","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Retained placenta (RP) is a common reproductive disorder with complex etiology and pathogenesis, affecting approximately 8% of dairy cows during the periparturient period. Macrophages constitute 20-25% of all leukocytes at the maternal-fetal interface and coordinate several processes critical for fetal membrane expulsion, including tissue remodeling, induction of apoptosis in damaged cells, and immune activation. This study aimed to investigate the morphological changes at the maternal-fetal interface, as well as the quantity, distribution, and polarization of caruncle macrophages in cows with and without RP. Furthermore, we discuss the potential association between macrophage alterations and histopathological changes in placental tissue of RP cows. A total of 80 Holstein dairy cows (parity, 2-4) were enrolled in this study. Blood samples were collected at -7 d before the expected calving date (-7D), at calving (0h), at 12h postpartum (12h) and at 7 d postpartum (7D). Placental tissue samples were collected within 30 min after parturition. Based on whether the placental membranes were expelled within 12 h postpartum, cows were classified retrospectively into normal expulsion (NE) (n = 6) and RP (n = 6) groups. Picrosirius red staining, along with elevated mRNA and protein levels of Collagen III, indicated enhanced collagen fiber deposition in caruncle tissue. In addition, the mRNA expression of matrix metalloproteinases (MMP-2 and MMP-9) was downregulated in RP tissues, while TIMP-1 was upregulated. Compared with normal expulsion cows, the apoptosis index, as well as the protein and mRNA levels of pro-apoptotic factors (BAX, Caspase-3, Caspase-8) were lower in cows with RP, and the anti-apoptotic factor (BCL2) was higher, indicating reduced apoptosis in the caruncle tissue from RP cows. In both the serum and tissues, we observed lower levels of chemotactic factors (CXCL1 and MCP-1) in RP cows, alongside increased IL-10 (an immunosuppressive factor) and decreased IL-1β (an immune-stimulatory factor). The downregulated protein and mRNA abundance of the macrophage marker CD68, consistent with reduced presence of CD68&lt;sup&gt;+&lt;/sup&gt; cells observed through immunofluorescence, revealed low numbers of caruncle macrophages in cows with RP. Further, the caruncles tissue of RP cows displayed significant alterations in the distribution of CD68&lt;sup&gt;+&lt;/sup&gt; macrophages, with reduced infiltration into trophoblast cells. Regarding macrophage phenotypic changes in RP cows, the greater protein and mRNA expression of M2 polarization markers (CD206, IL-10, IL-6, and TGF-β) along with greater numbers of CD206&lt;sup&gt;+&lt;/sup&gt;/CD68&lt;sup&gt;+&lt;/sup&gt; cells detected through immunofluorescence indicated that macrophage polarization phenotype in the caruncles of RP cows shifted predominantly toward M2 phenotype. In contrast, RP cows exhibited lower protein and mRNA levels of M1 polarization markers (CD86, iNOS, IL-1β, and NF-κB), as well as reduced numbers of CD86&lt;sup&gt;+&lt;/sup&gt;/CD","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between prolactin and local regulation of the mammary gland.","authors":"P Lacasse","doi":"10.3168/jds.2025-26358","DOIUrl":"https://doi.org/10.3168/jds.2025-26358","url":null,"abstract":"<p><p>Biological mechanisms exist to adjust milk output to demand. Experiments, where different milking frequencies were used within the mammary gland, have shown that milk production is only increased in the frequently milked half during a unilateral increase in milk frequency. Similarly, cessation of milking or mastitis of one quarter will induce the involution process in that quarter only. These data strongly suggest that milk production is regulated at the level of the mammary gland. However, other factors, such as pregnancy, bST administration, and photoperiod, also indicate the presence of a systemic regulation of milk secretion. Moreover, the linkages between local and systemic regulations are poorly understood. We have identified 3 potential links between local and systemic regulation: milking and suckling induce the release of hormones; modulation of the receptivity of the mammary gland to the systemic lactogenic signal; and secretion in milk of inhibitory substances that modulate response to systemic regulation. Therefore, a series of experiments was initiated to examine these potential linkages between local and systemic regulations. A first experiment provided no evidence that additional milking-induced hormone release episodes contribute to the stimulating effect of increasing milking frequency during an established lactation. Conversely, we found evidence that both milking frequency and incomplete milking modulate mammary gland responsiveness to PRL, and this is likely to contribute to their milk production response. We also found that the circulating level of prolactin affects the subsequent responsiveness to this hormone and that it is likely involved in the reduction of milk production after a short dry period. Finally, using an animal model that is unaffected by an inflammatory response, we found indications supporting the contention that milk stasis leads to the accumulation of one or more factors that trigger involution. Additionally, we determined that the milk levels of several microRNAs are affected by milk stasis and deserve further research to understand their roles in the control of mammary gland functions.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Information about production system and attitude toward sustainability: Do they influence the perception in consumers of sheep milk cheese?","authors":"I Etaio, T Manso, F J Pérez-Elortondo, B Gallardo, L Larrasoain, A Ruiz Mantecón, P Lavín, L J R Barron","doi":"10.3168/jds.2025-26494","DOIUrl":"https://doi.org/10.3168/jds.2025-26494","url":null,"abstract":"<p><p>Influence of information provided about the product on sensory perception was studied with consumers of sheep milk cheese. A group of 109 consumers from Vitoria-Gasteiz (Basque Country) and 115 consumers from Palencia (Castile and Leon) evaluated 2 cheeses from the Basque Country and 2 cheeses from Castile and Leon to rate their acceptability and to describe them by the \"check all that apply\" (CATA) method. Each cheese was evaluated 3 times throughout 2 sessions, presented with different codes and claims: the first time without information and, later, presented as cheese of milk from sheep in a grazing system and from sheep in nongrazing system, in a balanced design to avoid presentation bias. Acceptability scores were analyzed by ANOVA and HDS Tukey test to study the effect of the information provided, the origin of the cheese, the origin of the consumers, and the consumers' attitude for sustainability (they were previously classified as low-medium or high sensitivity toward sustainability). The CATA data were analyzed by Cochran's Q test to study the terms discriminating among samples and correspondence analysis was run to display a plot with samples and descriptive terms. Providing information about grazing or nongrazing system had an effect on acceptability of sheep cheeses. Samples presented as from sheep in a nongrazing system had lower acceptability but samples presented as from sheep in a grazing system did not receive higher acceptability scores than samples without information about grazing or nongrazing systems. These effects were observed in both consumers with low-medium and high sensitivity to sustainability, and among consumers from both locations. Differences in acceptability toward specific cheeses were observed between the 2 cities where the tests were run, and a possible familiarity effect among consumers from Palencia was observed, with a trend to score better cheeses from Castile and Leon. Perceived sensory characteristics were not influenced by the information provided, but samples presented from a nongrazing system were associated with \"seems industrial,\" whereas \"seems handmade\" and \"seems natural\" are close to samples presented as from grazing systems and without information about the grazing system. These findings suggest that informing the consumers about the sheep management can be of interest in cheeses made with milk from animals in a grazing system, whatever the consumers' sensitivity to sustainability is. Also, possible differences in liking due to familiarity with the product should be considered.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequence-based genome-wide association study and fine-mapping in German Holstein reveal new quantitative trait loci for health traits.","authors":"A M Križanac, C Reimer, J Heise, Z Liu, J E Pryce, J Bennewitz, G Thaller, C Falker-Gieske, J Tetens","doi":"10.3168/jds.2025-26328","DOIUrl":"https://doi.org/10.3168/jds.2025-26328","url":null,"abstract":"<p><p>We conducted a large GWAS of 11 health traits belonging to 3 trait complexes: (1) metabolic diseases, (2) infectious and noninfectious feet and claw disorders, and (3) udder-related traits in 100,809 to 180,217 German Holstein cows to investigate the genetic architecture and underlying biological mechanisms behind these complex traits. The GWAS identified 12,306 genome-wide significant variants across 10 traits. The new association signals were inspected with a Bayesian fine-mapping approach, leading to the discovery of 159 novel variants with high potential for causality. Variants were in known and novel regions for the traits studied, leading to a list of 53 novel candidate genes. Our study represents the largest whole-genome sequence GWAS for health traits so far, hence ensuring the power to detect meaningful variants, especially when enhanced with fine-mapping.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming Dairy for a Difference: Key Factors Influencing Electronic Word-of-mouth Intention and Purchase Intention for Social Enterprise Dairy Products.","authors":"Ching Yin Ip, Cheng-Ning Wu","doi":"10.3168/jds.2025-26597","DOIUrl":"https://doi.org/10.3168/jds.2025-26597","url":null,"abstract":"<p><p>This study explores the factors influencing purchase intention and electronic word-of-mouth (eWOM) intention for sustainable dairy products offered by social enterprises. Drawing on the theory of planned behavior, this research extends existing models by incorporating perceived adherence to social missions (PATSM), altruism, and Sustainable Development Goal (SDG) knowledge as key determinants. We collected data through an online survey of 651 Taiwanese consumers familiar with the social enterprise brand \"BetterMilk.\" The findings indicate that altruism and SDG knowledge indirectly influence purchase and eWOM intention through PATSM, outcome expectations, and subjective norms. By demonstrating that altruistic values and SDG knowledge can enhance consumer support for social enterprises, this study offers insights that may inform organizational strategies aimed at strengthening purchase and eWOM intention in the sustainable dairy sector. In addition, this research advocates for integrating sustainability education into entrepreneurship curricula. The implications highlight the potential for social enterprises in the dairy industry to align profitability with purpose, thereby contributing to a more sustainable marketplace.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yak milk improves retinoic acid-induced osteoporosis by promoting intestinal calcium absorption.","authors":"Torkun Mamet, Yanping Guo, Xiaotong Li, Jingru Yang, Bo Zhao","doi":"10.3168/jds.2023-24359","DOIUrl":"https://doi.org/10.3168/jds.2023-24359","url":null,"abstract":"<p><p>Yak milk is a unique nutritional quality milk with health-promoting benefits. In this study, we investigated the effect of yak milk on intestinal calcium absorption in mice with retinoic acid-induced osteoporosis (OP) and in the Caco-2 cell model. Results showed that compared with the model group, the apparent calcium absorption rate increased from 35.93% to 51.84% and 49.41% for the low-dose yak milk group (L-YM) and high-dose yak milk group (H-YM), respectively, and the calcium accumulation rate increased from 26.14% to 44.09% and 42.66%, respectively. However, no improvement was observed in the alendronate sodium (ALN) group. The expression of the transient receptor potential vanilloid 6 (TRPV6) calcium channel in the yak milk treatment group was 2.7 (L-YM) and 3.5 (H-YM) times higher than that in the model group, implying that TRPV6 channel was the main transport pathway in the small intestine. Furthermore, in vitro calcium absorption assays indicated that yak milk enhanced intestinal Ca²⁺ absorption more effectively than CaCl₂ by regulating the TRPV6 channel. These data suggest that yak milk promotes calcium absorption by upregulating the expression of the TRPV6 calcium channel, thereby improving OP. This study highlights the importance of yak milk as a dietary factor for bone health.</p>","PeriodicalId":354,"journal":{"name":"Journal of Dairy Science","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}