Pest Management Science最新文献

{"title":"Biocontrol activity and antifungal mechanisms of volatile organic compounds produced by Trichoderma asperellumXY101 against pear Valsa canker","authors":"Yang Zhang, Li Wu, Ying Lu, Bo Li, Zhaoyang Jin, Jun Wang, Ruxiao Bai, Qifeng Wu, Qinglu Fan, Jian‐Hong Tang, Feihu Yin, Yujian He","doi":"10.1002/ps.8837","DOIUrl":"https://doi.org/10.1002/ps.8837","url":null,"abstract":"BACKGROUNDSynthetic fungicides raise environmental and health concerns, and microorganisms are emerging as promising natural antagonists in plant protection. This study evaluated strain XY101 and its volatile organic compounds (VOCs) as biocontrol agents against pear Valsa canker caused by <jats:italic>Vasal pyri</jats:italic>, aiming to identify the antifungal mechanisms and key compounds.RESULTSThe biocontrol fungus was identified as <jats:italic>Trichoderma asperellum</jats:italic>. <jats:italic>Trichoderma asperellum</jats:italic> XY101 strongly suppressed the mycelial growth of <jats:italic>Vasal pyri</jats:italic> on culture plates. A colonization assay indicated that the VOCs produced by <jats:italic>T</jats:italic>. <jats:italic>asperellum</jats:italic> XY101 significantly reduced the pathogenicity of <jats:italic>Vasal pyri</jats:italic> on detached pear twigs, with an inhibition rate of 78.96%. Scanning electron microscopy (SEM) and laser confocal microscopy revealed that VOCs produced by <jats:italic>T</jats:italic>. <jats:italic>asperellum</jats:italic> XY101 caused abnormal changes in mycelial ultrastructure and damaged the integrity of fungal cell membrane. The results of headspace solid‐phase microextraction‐gas chromatography–mass spectrometry (HS‐SPME‐GC–MS) analysis showed the detection of 24 possible VOCs produced by strain XY101, with 3,7‐dimethyl‐1‐octanol and 1‐octen‐3‐ol identified as the primary antagonistic VOCs affecting pear Valsa canker. Transcriptome analysis demonstrated that these VOCs modulated gene expression in pathogenic fungal strains related to metabolism, membrane damage, pathogenicity, and resistance.CONCLUSION<jats:italic>Trichoderma asperellum</jats:italic> XY101 and its VOCs, 3,7‐dimethyl‐1‐octanol and 1‐octen‐3‐ol, showed effective inhibitory effects against <jats:italic>Vasal pyri</jats:italic>, providing a basis for the development of VOCs‐based biological fumigants to manage pear Valsa canker. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"17 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying key parameters for reliable assessment of entomopathogenic nematodes viability as affected by spray application stress‐related factors","authors":"Roberto Beltrán‐Martí, Marco Resecco, Elena Gonella, Sofía Victoria Prieto, Marco Pittarello, Cruz Garcerá, Patricia Chueca, Alberto Alma, Fabrizio Gioelli, Marco Grella","doi":"10.1002/ps.8847","DOIUrl":"https://doi.org/10.1002/ps.8847","url":null,"abstract":"BACKGROUNDConventional pesticide application equipment (PAE) is used to apply entomopathogenic nematode (EPN)‐based bioinsecticides, but their closed hydraulic systems could raise the temperature of the spray mixture up to 40 °C, potentially harming EPN, since temperatures above 30 °C can immobilize nematodes, reducing their infective capacity. This study aimed to identify the most suitable method to evaluate EPN viability under the effects of PAE technology.RESULTSThree EPN species—<jats:italic>Heterorhabditis bacteriophora, Steinernema feltiae,</jats:italic> and <jats:italic>Steinernema carpocapsae</jats:italic>—were exposed to thermal stress (10, 20, 30, and 40 °C for 270 min) to simulate spray application conditions. Three viability evaluation methods were compared: prodding stimulation, NaCl chemical stimulation, and no stimulation. Viability was measured by two parameters depending on the assessment method: % actively EPN moving (activity), or % total live EPN, both actively moving and immobile (survival). Additionally, a novel parameter estimating non‐lethal stress (Δ<jats:sub>nl s</jats:sub>) was defined by measuring the live but inactive EPNs. NaCl stimulation was optimized comparing different concentrations and durations and then set at 0.1 g mL<jats:sup>−1</jats:sup> for 1 min. Temperature significantly affected EPN viability over time. Temperatures around 20 °C preserved optimal conditions, and above 30 °C negatively affected EPN viability, with mortality close to 80% within 90 min at 40 °C. Prodding (measuring survival) yielded higher viability compared to NaCl and no stimulation, which measured activity. Non‐lethal stress parameter increased accordingly to stress increment showing potential as EPN stress‐marker.CONCLUSIONThe study concluded that combined measurement of survival, activity and non‐lethal stress should be considered in EPN viability assessments when designing PAE to ensure high efficacy of biocontrol agents. © 2025 The Author(s). <jats:italic>Pest Management Science</jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"9 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgenic cowpea conferring insect resistance and glyphosate tolerance","authors":"Haiyan Lin, Haozhi Zhong, Hongyu Gao, Chaoyang Lin, Ting Zheng, Chao Xu, Yuxuan Ye, Zhicheng Shen","doi":"10.1002/ps.8863","DOIUrl":"https://doi.org/10.1002/ps.8863","url":null,"abstract":"BACKGROUNDCowpea is a favorite vegetable around the world. In China, the tender pods of cowpea are the primary edible part. However, cowpea is vulnerable to a variety of pests throughout its entire growth cycle. Lepidopteran insects, including the legume pod borer (LPB, <jats:italic>Maruca vitrata</jats:italic>), beet armyworm (BAW, <jats:italic>Spodoptera exigua</jats:italic>), and tobacco cutworm (TCW, <jats:italic>Spodoptera litura</jats:italic>), are the major pests to cowpea production. Conventional pest management strategies predominantly rely on chemical pesticides, which not only increase production costs but also raise critical concerns regarding food safety and environmental sustainability. Genetically modified (GM) technology offers a promising solution of pest management by introducing insect resistance trait into plants. Unlike transgenic corn and soybean, transgenic cowpea conferring insect resistance has not been fully explored and utilized.RESULTSIn this study, a Cry1Ab‐Vip3A fusion gene, <jats:italic>C1V3</jats:italic>, was successfully introduced into the cowpea genome. Transgenic cowpea lines with resistance to broad‐spectrum lepidopteran pests were obtained by an improved transformation method using glyphosate as selection. A promising line, CAP5, selected from 37 transgenic events, was characterized as a single T‐DNA copy event with single insertion site at the Chr5 of cowpea genome. Comprehensive evaluation for insect resistance and herbicide tolerance indicated that CAP5 was highly resistant to BAW, LPB and TCW and exhibited strong tolerance to glyphosate, underscoring its application potential as an insect‐resistant and herbicide‐tolerant cowpea event.CONCLUSIONTransgenic cowpea line CAP5 expressing Cry1Ab‐Vip3A fusion protein and CP4‐EPSPS is highly resistant to BAW, TCW and LPB, and strongly tolerant to glyphosate. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"37 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence-based pest control","authors":"Konstantinos Mavridis, Vasiliki Evangelou, Alexandra M Grigoriadou, Dimitrios P Papachristos, John Vontas","doi":"10.1002/ps.8849","DOIUrl":"https://doi.org/10.1002/ps.8849","url":null,"abstract":"The invasive fall armyworm (<i>Spodoptera frugiperda</i>, FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence-based pest control strategies. In this study, we analyzed target-site insecticide resistance mutations in FAW populations from Greece to inform pest control strategies. In addition, DNA barcoding through cytochrome oxidase subunit 1 (<i>COI</i>) gene sequencing was used to trace the pest's geographic origin and potential invasion pathways.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"27 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative fumigation technologies for Bursaphelenchus xylophilus-infected woods: reducing reliance on methyl bromide","authors":"Hao Yang, Yuqi Hua, Tao Liu, Siming Fang, Wei Gu, Juan Shi","doi":"10.1002/ps.8821","DOIUrl":"https://doi.org/10.1002/ps.8821","url":null,"abstract":"Pine wilt disease (PWD), caused by the pine wood nematode (PWN) <i>Bursaphelenchus xylophilus</i> (Steiner &amp; Buhrer) Nickle, poses a significant global threat to pine forests. This study investigated the nematicidal efficacy of four fumigants—methyl bromide (MB), phosphine (PH₃), sulfuryl fluoride (SF), and methyl isothiocyanate (MITC)—applied both individually and in combination (PH₃+MB, SF+MB, MITC+MB, PH₃+SF, PH₃+MITC, and MITC+SF) against PWN.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"28 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifenthrin resistance in Dalbulus maidis (Hemiptera: Cicadellidae): inheritance, cross-resistance, and stability","authors":"Gabriel Silva Dias, Eduardo Perkovski Machado, Matheus Gerage Sacilotto, Leonardo Vinicius Thiesen, Celso Omoto","doi":"10.1002/ps.8848","DOIUrl":"https://doi.org/10.1002/ps.8848","url":null,"abstract":"Pyrethroid insecticides have been a primary strategy for managing <i>Dalbulus maidis</i> (Hemiptera: Cicadellidae) in Brazil. Howeve, failures in the control of <i>D. maidis</i> with pyrethroids have been reported. In this study, we selected a bifenthrin-resistant strain of <i>D. maidis</i> under laboratory cage conditions to investigate the inheritance pattern of resistance, cross-resistance to other insecticides, and resistance stability.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"37 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"History and future perspectives of spinosad for mosquito control","authors":"Tianyun Su, Hongxia Liu","doi":"10.1002/ps.8820","DOIUrl":"https://doi.org/10.1002/ps.8820","url":null,"abstract":"Since the initial discovery of the soil-dwelling actinomycete <i>Saccharopolyspora spinosa</i> in 1982, its metabolites spinosyns and modified spinosoids have been developed to control a wide variety of arthropod pests of economic importance. The most intensively studied are spinosad consisting of natural spinosyn A and D, as well as spinetoram comprising modified spinosyn J and L. The unique mode-of-action, high bioactivity and generally benign environmental safety profile of spinosyns add significant value to this class of pesticides of natural origin. Currently, products based on spinosad and spinetoram are available in &gt;85 countries since initial global registration in 1996. In the US, spinosad and spinetoram are found in &gt;80 registered pesticide products, mostly being used on agricultural crops, ornamental plants, stored grains, urban environments, and pets. Some spinosad products are approved for use in organic farming. Since the earliest recognition and registration of spinosad products for mosquito control in the 2000s, numerous customized formulations have been developed and registered for controlling different mosquito species in diverse habitats. Thus far, satisfactory bioactivities and effectiveness against mosquitoes by available products have been documented worldwide. At the same time, concerns over nontarget aquatic invertebrate fauna have gradually emerged when considering spinosad as a relatively selective pesticide. Resistance and cross-resistance following repeated applications, and their management are of pressing concern. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"135 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What you eat is what we need: using ants to detect spotted lanternfly (Lycorma delicatula) DNA","authors":"Wei-Jiun Lin, Fang-Ling Chloe Liu, Xun-Yi Huang, Alejandro I. Del Pozo-Valdivia, Tracy C. Leskey, Chin-Cheng Scotty Yang","doi":"10.1002/ps.8814","DOIUrl":"https://doi.org/10.1002/ps.8814","url":null,"abstract":"Early detection of invasive species such as the spotted lanternfly (SLF, <i>Lycorma delicatula</i>) is critical for effective management including eradication efforts and limiting further spread. SLF excretes honeydew containing detectable DNA, providing a unique opportunity to leverage environmental DNA (eDNA) for its detection. This study introduces the ant-derived DNA (antDNA) approach, utilizing ants as ‘honeydew samplers’ to detect SLF DNA. We validated the effectiveness of this method through three experiments.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"68 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-level genome assembly of Dendrothrips minowai and genomic analysis highlights distinct adaptations to high polyphenols in tea plants","authors":"Chunli Xiu, Dehai Zhao, Jiahui Zhang, Haitao Liu, Yusheng Wang, Hangwei Liu, Xiaoming Cai, Zongxiu Luo, Lei Bian, Nanxia Fu, Li Zhou, Zongmao Chen, Zhaoqun Li","doi":"10.1002/ps.8781","DOIUrl":"https://doi.org/10.1002/ps.8781","url":null,"abstract":"<i>Dendrothrips minowai</i> Priesner, a significant pest in tea-producing regions of Asia, particularly China, damages tea plants (<i>Camellia sinensis</i>) by feeding on their tender leaves rich in polyphenols. Seven assembled genomes from the order Thysanoptera are currently available.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"13 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory role of the Bxy‐gld‐1 in meiosis and oocyte development of Bursaphelenchus xylophilus","authors":"Hudie Shao, Quan Li, Jing Chen, Wenxin Hu, Yuxin Zou, Hengliang Liu, Jinghan Wang, Liqun Bai, Jiafu Hu","doi":"10.1002/ps.8846","DOIUrl":"https://doi.org/10.1002/ps.8846","url":null,"abstract":"BACKGROUNDInvestigating the cell fate of the germline in <jats:italic>Bursaphelenchus xylophilus</jats:italic> can reveal the biological characteristics of its reproductive processes, which is crucial for formulating more targeted control strategies against pine wilt disease. However, the molecular regulatory mechanisms governing cell fate in the germline of <jats:italic>B. xylophilus</jats:italic> remain unclear.RESULTSIn this study, we performed functional validation of the <jats:italic>Bxy‐gld‐1</jats:italic> gene in <jats:italic>B. xylophilus</jats:italic> through bioinformatics analysis, <jats:italic>in situ</jats:italic> hybridization, and RNA interference (RNAi) combined with DAPI staining techniques. Bioinformatics research indicates that the <jats:italic>Bxy‐gld‐1</jats:italic> gene is highly similar to the <jats:italic>gld‐1</jats:italic> gene in the model organism <jats:italic>Caenorhabditis elegans</jats:italic>. <jats:italic>In situ</jats:italic> hybridization results demonstrate that the <jats:italic>Bxy‐gld‐1</jats:italic> gene is expressed in the gonadal regions of nematodes at all developmental stages. RNAi results show that silencing the <jats:italic>Bxy‐gld‐1</jats:italic> gene delays the transition of germline cells from mitosis to meiosis in <jats:italic>B. xylophilus</jats:italic> and can cause abnormal oocyte development in female nematodes, leading to the occurrence of small oocytes. Additionally, lifespan assays revealed that the longevity of virgin female and male nematodes in the RNAi‐treated group was significantly reduced.CONCLUSIONSThis study identifies the <jats:italic>Bxy‐gld‐1</jats:italic> gene as a key regulator of germline cell fate in <jats:italic>B. xylophilus</jats:italic>, advancing our understanding of its reproductive mechanisms and providing a basis for targeted therapeutic development. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"70 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143867029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}