Pest Management Science最新文献

{"title":"Field evaluation of an agricultural weed detector using YOLO image recognition: background conditions affect detection performance.","authors":"Saeko Matsuhashi,Ryo Sugiura,Motoaki Asai,Hidenori Asami,Yusuke Kowata,Yuki Akamatsu,Kozue Sasaki,Namiko Yoshino,Nozomi Ihara,Akira Koarai","doi":"10.1002/ps.70009","DOIUrl":"https://doi.org/10.1002/ps.70009","url":null,"abstract":"BACKGROUNDImage recognition tools for weed identification, such as smartphone applications, have the potential to enhance user knowledge, provide early invasive weed alerts, and enable site-specific weed management. Although numerous studies have reported product development and model accuracy, few have evaluated these tools in practical environments beyond developmental settings. In this study, we developed a weed detector using the You Only Look Once (YOLO) v3 object detection algorithm to identify six noxious weed species. Specifically, we examined the effects of: (i) image collection locations, (ii) target backgrounds, and (iii) camera devices on detection accuracy, assessing applicability through field verification at 68 sites across Japan and controlled garden experiments.RESULTSDetection success was influenced by the background of the target species in images, with significant interaction effects observed between background and target species on detection outcomes. In the most affected combination (background: tray; species: Ipomoea lacunosa), the average precision (AP) value decreased by ~0.2 compared with the other conditions. AP values in field tests were lower than those from test data resembling training data, with no correlation between AP values from test data sets and field verification. No clear effects of land use or camera devices on detection success were detected.CONCLUSIONThis study highlights the importance of background in image-based weed detection and identifies limitations in detector applicability. Our findings are expected to support more-efficient development and underscore the importance of sharing applicability data for improved weed detection tools. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"14 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A deletion upstream of the degron of AcIAA27 leads to auxinic herbicide resistance in Arctotheca calendula.","authors":"Yuanlin Qi,Mahima Krishnan,Matthew Tucker,Peter Boutsalis,Christopher Preston","doi":"10.1002/ps.70000","DOIUrl":"https://doi.org/10.1002/ps.70000","url":null,"abstract":"BACKGROUNDTwo populations of Arctotheca calendula (capeweed) from near Naracoorte, South Australia, were not controlled by 2,4-D. These populations were investigated for resistance to 2,4-D and other auxin herbicides. Aux/IAA genes were investigated for potential mutations.RESULTSDose response experiments confirmed both populations were resistant to 2,4-D and also cross-resistant to MCPA, dicamba, triclopyr and picloram. The level of 2,4-D resistance in the populations was unchanged by pretreatment with the P450 inhibitor, malathion. Compared to the susceptible populations, a 12 bp deletion and a point mutation (Val116Asp) were identified upstream of degron region in AcIAA27 of R1, located 29 and 19 amino acids away from the degron. However, R2 did not have this mutation. Overexpression of AcIAA27Δ12 and AcIAA27Δ12+D in Arabidopsis increased survival of plants treated with 2,4-D, dicamba, fluroxypyr and picloram, compared with controls. There was no significant difference in the level of resistance to auxin herbicides between Arabidopsis lines expressing AcIAA27Δ12 and AcIAA27Δ12+D.CONCLUSIONOur study demonstrated that resistance to 2,4-D and other auxin herbicides in capeweed population R1 is due to a deletion in AcIAA27. However, the Val116Asp mutation did not appear to contribute to resistance. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"55 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lethal effects of allyl isothiocyanate on the egg masses of flighted spongy moth complex Lymantria dispar japonica (Lepidoptera: Erebidae).","authors":"Shunya Murase,Keisuke Matsui,Hiromi Asai,Daisuke Hayasaka,Takuo Sawahata","doi":"10.1002/ps.8981","DOIUrl":"https://doi.org/10.1002/ps.8981","url":null,"abstract":"BACKGROUNDThe flighted spongy moth complex (FSMC) lays egg masses not only on host plants but also various artificial structures, such as, cargo, vehicles, and ships. Thus, preventing the transmission of these egg masses is an international challenge. Their eggs are covered with thick hair covering that act as a barrier to insecticides thus making them ineffective. A volatile gas may be able to carry toxic substances past this barrier to the eggs. In this study, we evaluated the insecticidal effect of the highly volatile allyl isothiocyanate (AITC) on Lymantria dispar egg masses and compared it with that of two conventional insecticides containing permethrin, fenpropathrin, and clothianidin.RESULTSIn the two conventional insecticide treatments, approximately 50% of the eggs survived when the hairy covering was not removed. In contrast, all eggs, with or without hairy covering, did not hatch after AITC treatment. The LC50 and LC90 values of AITC against them were 37.3 ± 5.4 and 100.9 ± 11.8 ppm, respectively. Our results show that AITC at 35000 ppm can eradicate FSMC egg masses.CONCLUSIONAITC has a lethal effect against FSMC egg masses and may be applied for the extirpation of eggs/egg masses of various other organisms, besides FSMC. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"14 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High value-added application of renewable natural forest product 3-carene: discovery of diacylthiosemicarbazide compounds as potential laccase inhibitors.","authors":"Yucheng Cui,Wengui Duan,Guishan Lin,Yao Chen,Yuzhi Lu,Weiming Chen,Hao Li","doi":"10.1002/ps.70004","DOIUrl":"https://doi.org/10.1002/ps.70004","url":null,"abstract":"BACKGROUNDThe application of pesticides remains the most effective method for managing crop pests, weeds and diseases. However, owing to issues such as resistance to pesticides, environmental pollution, health problems and consumer demand for food safety, it is highly desirable to research and develop green and environmentally-friendly novel pesticides.RESULTSTwenty-three novel 3-carene-derived diacylthiosemicarbazide compounds were synthesized and characterized. The absolute configuration of the target compounds was also confirmed by single crystal X-ray diffraction. The tautomerization phenomenon of the target compounds was investigated using theoretical calculations. The biological activity assays showed that compounds DAT21~DAT23 not only exhibited excellent antifungal activity, but also demonstrated outstanding herbicidal activity and significant laccase inhibitory activity. The cytotoxicity results showed that compounds DAT21 and DAT23 displayed lower cytotoxicity towards three normal cells at a concentration of 10 mg L-1. The action mechanism was further conducted by molecular docking and the chelating property of the target compound with copper ion (Cu2+).CONCLUSIONThese results revealed that compounds DAT21~DAT23 may exert excellent pesticidal activity by inhibiting laccase. Therefore, compounds DAT21~DAT23 could serve as promising novel green pesticidal lead compounds and deserve further research. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"14 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144311620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet-induced gut microbiota shifts in grasshoppers: ecological implications for management and adaptation under varying grazing intensities.","authors":"Tingting Shen, Qian Wu, Yao Tan, Xin Ju, Guodong Han","doi":"10.1002/ps.8953","DOIUrl":"https://doi.org/10.1002/ps.8953","url":null,"abstract":"<p><strong>Background: </strong>Grasshoppers play a keystone role in health and stability in grassland ecosystems, which are critical for biodiversity and ecological services. Despite this, in the context of the desert steppe, there remains a paucity of knowledge regarding the response of Calliptamus abbreviatus to variations in grazing intensity and the subsequent impact on its gut microbiome and dietary diversity. The present study evaluated the impact of variations in sheep grazing intensity on the composition of plant species, which in turn influences the diet and intestinal microbiota of the grasshopper C. abbreviatus, an abundant species in these ecosystems.</p><p><strong>Results: </strong>We found that increased grazing intensity has significant impacts on plant species composition and diversity, reduces grasshopper dietary diversity, increases reliance on Allium tenuissimum, and alters gut microbial community structure. Notably, plant species composition changes associated with high grazing intensity decrease Actinobacteria and Frankia but increase Proteobacteria, potentially affecting grasshopper adaptability and nutrient absorption. Procrustes analysis revealed a significant congruence between diet and gut microbiota, and the plant species changes associated with grazing serve as a pivotal point in the enterotype differentiation of grasshoppers, with enterotype 2 potentially endowing grasshoppers with enhanced nutrient absorption and transport capabilities under grazing, helping them adapt to environmental changes.</p><p><strong>Conclusion: </strong>Our findings emphasize that the diet and structure and function of grasshopper gut microbial communities are altered by the quality and quantity of food resources. This study provides insights into the long-term interactions between insects and their environment, and these insights are crucial for sustainable grassland management and pest control strategies. © 2025 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Side effects of pesticides used in organic and conventional tomato crops on Dolichogenidea gelechiidivoris, a parasitoid of Tuta absoluta.","authors":"Pablo Urbaneja-Bernat, Jordi Riudavets, Gabriele Caporusso, Judit Arnó","doi":"10.1002/ps.8982","DOIUrl":"https://doi.org/10.1002/ps.8982","url":null,"abstract":"<p><strong>Background: </strong>Dolichogenidea gelechiidivoris is a key biological control agent of Tuta absoluta, one of the most destructive pests in tomato crops worldwide. As part of integrated pest management (IPM) programs, pesticides are commonly applied to control multiple pests. However, their compatibility with parasitoids such as D. gelechiidivoris remains poorly understood. This study evaluated the impact of eight commonly used pesticides on D. gelechiidivoris through four exposure routes relevant to field conditions.</p><p><strong>Results: </strong>Among the pesticides tested, spinosad and cyantraniliprole were the most toxic, causing up to 90% adult mortality via fresh residues and topical exposure. These compounds also showed high ingestion toxicity and completely suppressed parasitoid emergence from parasitized hosts. Conversely, sulfur, spiromesifen and spirotetramat had a low impact on adult survival, and allowed parasitoid emergence rates similar to the control. Notably, Bacillus thuringiensis significantly reduced female egg load without increasing adult mortality, indicating a sublethal reproductive effect. Overall, pesticide impact varies depending on the exposure route, with contact and topical exposure generally leading to higher mortality than ingestion or effects on immature stages.</p><p><strong>Conclusion: </strong>This study highlights substantial variation in the side effects of IPM-compatible pesticides on D. gelechiidivoris. While some products were highly disruptive, others proved more selective and compatible with biological control. These findings support more informed pesticide selection in tomato IPM, balancing effective T. absoluta control with the conservation of key parasitoids. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analyses provide molecular insights into host immune responses and metabolic disruption in Spodoptera frugiperda parasitized by Pyemotes zhonghuajia.","authors":"Liang Wang,Xue-Lin Li,Di Fan,Yi-Chai Chen,Yan-Fei Song,Ji-Feng Hu,Guy Smagghe,Jian-Feng Liu,Mao-Fa Yang","doi":"10.1002/ps.8969","DOIUrl":"https://doi.org/10.1002/ps.8969","url":null,"abstract":"BACKGROUNDHost-parasitoid interactions are intricate biological processes in which the host's immune system mounts diverse physiological responses to counter parasitic infection. This study presents a comprehensive investigation into the molecular responses underlying the parasitism of the fall armyworm (Spodoptera frugiperda) by Pyemotes zhonghuajia, a parasitic mite with promising potential as a biocontrol agent.RESULTSUsing an integrated multi-omics approach-combining lipidomics, transcriptomics, and proteomics-we examined the effects of P. zhonghuajia parasitism on S. frugiperda at sublethal (10 mites) and lethal (40 mites) densities. Lipidomic analysis revealed significant alterations across 32 lipid classes, including a marked reduction in triglycerides and membrane-associated lipids, indicating impaired energy metabolism and disrupted cellular integrity. Transcriptomic analysis identified 1287 differentially expressed genes (DEGs) under sublethal parasitism and 1774 DEGs under lethal parasitism, with key pathways involved in immune response, detoxification, and lipid metabolism. Proteomic analysis quantified over 4000 proteins, identifying 74 and 247 differentially expressed proteins (DEPs) under sublethal and lethal parasitism, respectively. Integrated analysis highlighted key immune-related proteins, such as small heat shock proteins and C-type lectins, as central to the host immune response.CONCLUSIONThese findings shed light on how P. zhonghuajia manipulates host immunity and metabolism, identifying potential biomarkers for pest management and contributing to the development of sustainable, RNAi-compatible biocontrol strategies. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"33 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel boron-containing anthranilic diamide derivatives: design, synthesis, and exploration of the action mode against lepidopteran insects.","authors":"Weibin Xie, Peizhuo Li, Jinmin Peng, Yue Chen, Jieyin Liu, Xiaochen Wang, Zhiguang Yuchi, Liangnian He, Yuxin Li","doi":"10.1002/ps.8979","DOIUrl":"https://doi.org/10.1002/ps.8979","url":null,"abstract":"<p><strong>Background: </strong>Diamide insecticides targeting insect ryanodine receptors (RyRs) have demonstrated exceptional efficacy against lepidopteran insects while exhibiting low toxicity to non-target organisms. However, their widespread use over the past decade has led to the rapid development of pest resistance, diminishing their long-term effectiveness. Boron, an essential micronutrient for plant growth, has attracted attention in drug design, owing to its unique chemical properties.</p><p><strong>Results: </strong>To develop new insecticides with novel mechanisms for effective lepidopteran insect control, 19 boron-containing anthranilic diamide derivatives were designed and synthesized based on the scaffold of chlorantraniliprole (CHL), using active fragment splicing and virtual screening strategies. The structures of the target compounds were confirmed using proton (¹H) nuclear magnetic resonance (NMR), carbon-13 (¹³C) NMR, fluorine-19 (¹⁹F) NMR, and high-resolution mass spectrometry (HRMS). Preliminary bioassays revealed that most compounds exhibited good-to-excellent insecticidal activity against Mythimna separata and Spodoptera frugiperda. Among them, compound 5b showed remarkable activity against M. separata (60% mortality at 0.1 mg/L), outperforming CHL (40%) at the same concentration. Calcium imaging technique, time-lapse [Ca²⁺]_ER measurements, and transcriptomic analyses indicated that RyRs may serve as primary targets of compound 5b. Molecular docking further predicted the mode of binding of 5b within the RyRs protein.</p><p><strong>Conclusion: </strong>The boron-containing anthranilic diamide derivatives are promising candidates for developing next-generation insecticides, offering valuable insights into overcoming lepidopteran pest resistance. © 2025 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism and control efficacy of plasma-activated water seed treatment against bacterial fruit blotch in melon seedlings.","authors":"YuHe Chen,Ting Fan,NaNa Zhang,Shanshan Peng","doi":"10.1002/ps.8965","DOIUrl":"https://doi.org/10.1002/ps.8965","url":null,"abstract":"BACKGROUNDBacterial fruit blotch (BFB) of melons, induced by Acidovorax citrulli, is a severe seed-borne disease. Traditional chemical seed treatments risk environmental pollution and antibiotic resistance. Plasma-activated water (PAW), as an innovative, efficient, eco-friendly and nontoxic method for seed disinfection, demonstrates significant potential for application. However, the efficacy and mechanisms of PAW in seed treatment for controlling BFB in melons remain poorly understood.RESULTSThis study investigated the efficacy of PAW produced by underwater jet-array gas-liquid plasma discharge in controlling BFB caused by A. citrulli. Soaking seeds in PAW (generated by a 1-h plasma discharge) for 4 h significantly decreased the disease incidence and severity, with a 97.2% decrease in the disease index compared to the control group. Furthermore, plant growth metrics showed marked improvement, including increases of 5.51% in plant height, 5.04% in stem diameter, 18.10% in leaf area and 27.58% in root length. Scanning electron microscopy, Fourier transform infrared and X-ray photoelectron spectroscopy analyses revealed that reactive oxygen/nitrogen species (RONS) in acidic PAW etched seed coats, suppressed bacterial growth and altered chemical bonds (reducing C─C/C─H while enhancing C─N/C─O), collectively improving seed activity.CONCLUSIONSThis study offers an effective and practical strategy for managing BFB in seedlings. PAW seed treatment reduces crop damage from bacterial diseases, enhances seed physiological activity and lowers environmental risks compared with conventional chemical treatments. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"25 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The t‐SNARE domain‐containing soybean cyst nematode HgSNARE1 is an effector that interacts with the GmSNAP18–GmSHMT08–GmPR1‐15 module and shows avirulence to soybean","authors":"Liuping Zhang, Jie Zhao, Lingan Kong, Wenkun Huang, Huan Peng, Deliang Peng, Feng Yu, Shiming Liu","doi":"10.1002/ps.8978","DOIUrl":"https://doi.org/10.1002/ps.8978","url":null,"abstract":"BACKGROUNDSoybean cyst nematode (SCN, <jats:italic>Heterodera glycines</jats:italic>) poses a significant threat to soybean (<jats:italic>Glycine max</jats:italic> (L.)), causing huge yield losses annually in global soybean production. Although the two main soybean SCN‐resistant genes, <jats:italic>rhg1 GmSNAP18</jats:italic> and <jats:italic>Rhg4 GmSHMT08</jats:italic>, have been cloned, the SCN effectors have been identified and functioned rarely, and the molecular mechanisms of effectors interacting with soybean remain largely unknown. Here, we identified the SCN effector HgSNARE1 and investigated how it targeted the soybean GmSNAP18–GmSHMT08–GmPR1‐15 module, aiming to reveal a new molecular mechanism for SCN control.RESULTSIn this study, the t‐SNARE domain‐containing protein HgSNARE1 was identified as an effector from SCN that was expressed the highest at the third juvenile (J3) stage. Expression of <jats:italic>HgSNARE1</jats:italic> in soybean hairy roots promoted SCN resistance, indicating the avirulence function of HgSNARE1 to soybean. HgSNARE1 interacted with GmSNAP18 via the t‐SNARE domain, with the last five amino acid residues (S<jats:sup>308</jats:sup>V<jats:sup>309</jats:sup>H<jats:sup>310</jats:sup>K<jats:sup>311</jats:sup>A<jats:sup>312</jats:sup>) in the C terminus playing an essential role in the interaction. GmSNAP18 interacted with both GmSHMT08 and a pathogenesis‐related (PR) 1 protein GmPR1‐15 (Glyma.15 g062400), and GmSHMT08 interacted with GmPR1‐15. Therefore, GmSNAP18, GmSHMT08 and GmPR1‐15 formed a module by pairwise interactions. However, neither GmSHMT08 nor GmPR1‐15 interacted with HgSNARE1. Furthermore, expression of <jats:italic>HgSNARE1</jats:italic> in soybean hairy roots stimulated expression of <jats:italic>GmPR1‐15</jats:italic>.CONCLUSIONThese findings reveal a novel molecular mechanism by which the SCN effector HgSNARE1 interacts with SCN‐resistant GmSNAP18 of the GmSNAP18–GmSHMT08–GmPR1‐15 module and promotes <jats:italic>GmPR1‐15</jats:italic> expression to confer avirulence, providing a potential target for the development of new SCN‐resistant germplasms, and innovative nematode management strategies. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"44 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}