Pest Management Science最新文献

{"title":"Antifungal phloroglucinol derivatives from Dryopteris fragrans (L.) Schott","authors":"Dongrui Zhang, Jiaxuan Wang, Yang Liu, Weicong Dai, Jiaqi Zhang, Xueqi Wang, Ji Zhang, Ying Chang","doi":"10.1002/ps.8836","DOIUrl":"https://doi.org/10.1002/ps.8836","url":null,"abstract":"Potatoes, a major economic crop, are significantly impacted by <i>Fusarium</i> dry rot, a prevalent postharvest disease. In <i>Dryopteris fragrans</i>, the research of phloroglucinol derivatives are mainly focused on antibacterial activity, while the inhibitory effects on phytopathogenic fungi have not been reported.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"219 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling, virtual screening, and enzymatic docking of trehalose 6-phosphate phosphatase and evaluation of the insecticidal effect of phthalimide, N-(p-tolylsulfonyl) on Aedes aegypti (Diptera: Culicidae)","authors":"Raquel Jemima Viana Lima, Fernando Berton Zanchi, Railton Marques de Souza Guimarães, Alexandre de Almeida e Silva","doi":"10.1002/ps.8841","DOIUrl":"https://doi.org/10.1002/ps.8841","url":null,"abstract":"<i>Aedes aegypti</i> Linnaeus is a medically important vector because of its role in transmitting several arboviruses. Trehalose-6-phosphate phosphatase (TPP), an enzyme from the trehalose pathway, was the focus of this study, which aimed to model it, perform molecular docking and select potential ligands to evaluate their larvicidal and adulticidal activity on the mosquito.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"108 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of ACCase W2027C mutation in Leptochloa chinensis (L.) Nees using RAA-CRISPR/Cas12a: a sensitive and visual tool for herbicide resistance diagnosis","authors":"Shu Liu, Ziheng Cao, Jiahao Lun, Chao Zhang, Lang Pan","doi":"10.1002/ps.8840","DOIUrl":"https://doi.org/10.1002/ps.8840","url":null,"abstract":"Prolonged application of ACCase-inhibiting herbicides, such as cyhalofop-butyl, has resulted in the widespread emergence of herbicide-resistant <i>Leptochloa chinensis</i> populations in paddy fields. Among the numerous target-site resistance (TSR) mutations in ACCase, the Trp-2027-Cys (W2027C) mutation is one of the most prevalent in <i>L. chinensis</i>. The increasing prevalence of herbicide-resistant <i>L. chinensis</i> highlights the critical need for rapid and precise diagnostic tools to detect resistance mutations.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"65 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of naturally occurring entomopathogens against Maladera formosae in corn–soybean rotated systems","authors":"Adrian Joseph Pekarcik, Christopher G Taylor, Amy Lynn Raudenbush, Kelley Jean Tilmon","doi":"10.1002/ps.8851","DOIUrl":"https://doi.org/10.1002/ps.8851","url":null,"abstract":"The Asiatic garden beetle, <i>Maladera formosae</i>, emerged as a significant early season pest of field corn in the Great Lakes region beginning in the mid-2000s. Management tools used broadly for annual white grub species, like seed- and soil-applied insecticides, are ineffective against or have not been evaluated specifically for <i>M. formosae</i>. Biological control, including entomopathogenic nematodes, is successful against <i>M. formosae</i> in turf systems. The goals of this study were to (i) isolate and identify nematode species from agricultural fields in northern Ohio with field histories of <i>M. formosae</i>, and (ii) evaluate their ability to infect <i>M. formosae</i> grubs in greenhouse and field trials.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"32 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic mutations and insecticide resistance in Diaphorina citri: a comparative study across Chinese citrus regions","authors":"Tian-Yuan Liu, Yu-Xi Zhou, Chen-Yang Yuan, Yi-Fan Gao, Jia-Yao Fan, Guo-Rui Yuan, Jin-Jun Wang, Wei Dou","doi":"10.1002/ps.8845","DOIUrl":"https://doi.org/10.1002/ps.8845","url":null,"abstract":"<i>Diaphorina citri</i>, a serious pest of citrus, causes significant economic losses due to its role in spreading Huanglongbing. Chemical treatment is the primary method for controlling <i>D. citri</i>. In recent years, <i>D. citri</i> has demonstrated varying resistance levels to a number of pesticides. Combination bioassay and amplicon sequencing approaches can provide a more accurate assessment of resistance in <i>D. citri</i>.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"2 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of natural l-phenylalanine-derived Amidohydrazide derivatives in ensuring agriculture production against phytopathogenic fungi†","authors":"Tian-Cheng Zhang, Hong-Bin Fang, Yu-Fei Gong, Wen-Fei Zhang, Jia-Cheng Liu, Jing Chang, Zhan-Fang Chen, Lu-Fei Zhao, Yu-Cheng Gu, Xuewen Hua","doi":"10.1002/ps.8856","DOIUrl":"https://doi.org/10.1002/ps.8856","url":null,"abstract":"Ensuring food security is fundamental to national security and an important guarantee for global peace and development. The use of fungicides represents an effective strategy in safeguarding sufficient food supply against phytopathogenic fungi; however, given the escalating pathogen resistance, there is an urgent need to develop new, highly efficient, and environmentally friendly green fungicides.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"15 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockout of silk fibroin genes in Plutella xylostella results in decreased fitness and performance","authors":"Rui‐Chang Niu, Qing‐Hui Zeng, Wen‐Jing Wang, Yi‐Jing Wang, Tong‐Xian Liu, Shi‐Ze Zhang","doi":"10.1002/ps.8852","DOIUrl":"https://doi.org/10.1002/ps.8852","url":null,"abstract":"BACKGROUNDThe ability of insects to spin silk is crucial for their survival, reproduction, and interactions with the environment. The diamondback moth (DBM), <jats:italic>Plutella xylostella</jats:italic>, a serious agricultural pest, relies on silk production, which influences its behavior and population dynamics.RESULTSThis study employed CRISPR/Cas9 technology to knock out three genes, that is, silk fibroin heavy chain (<jats:italic>PxFibH</jats:italic>), silk fibroin light chain (<jats:italic>PxFibL</jats:italic>), and fibrohexamerin (<jats:italic>PxP25</jats:italic>), to investigate their roles in silk gland development and related biological traits. We successfully generated <jats:italic>PxFibH</jats:italic>, <jats:italic>PxFibL</jats:italic>, and <jats:italic>PxP25</jats:italic> knockout mutants, which displayed defective cocoon formation and developed into naked pupae. Further analysis revealed significant alterations in silk gland structure and various biological parameters, including increased larval mortality, prolonged developmental time, reduced pupal weight, and shortened adult lifespan.CONCLUSIONSThese findings highlight the importance of silk fibroin genes in silk production and growth development in <jats:italic>P. xylostella</jats:italic>, positioning them as potential targets for innovative pest control strategies. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"17 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indole‐diterpenoids from an endophytic Penicillium brefeldianum F4a and their antifeedant and insecticidal activities against Spodoptera frugiperda","authors":"Yan Bai, Fengting Kong, Xiaoyu Song, Yafei Han, Yinzhou Jiang, Jiangchun Hu, Huaqi Pan","doi":"10.1002/ps.8842","DOIUrl":"https://doi.org/10.1002/ps.8842","url":null,"abstract":"BACKGROUND<jats:italic>Spodoptera frugiperda</jats:italic> (Lepidoptera: Noctuidae) is one of the world's major agriculture pests, causing significant crop damage and serious economic losses. As <jats:italic>S. frugiperda</jats:italic> has developed widespread resistance, environmentally friendly insecticides are urgently needed. Therefore, searching for natural antifeedant or insecticidal lead compounds has become imperative.RESULTSThree new indole diterpenoids, paspamine A (1), paspamine B (2), and paspalitrem D (3), along with nine known ones (compounds 4–12), were isolated from an endophytic <jats:italic>Penicillium brefeldianum</jats:italic> F4a. Their structures were identified through comprehensive spectroscopic data analysis [one‐dimensional (1D) and two‐dimensional (2D) nuclear magnetic resonance (NMR), high‐resolution electrospray ionization mass spectrometry (HR‐ESI‐MS), ultraviolet (UV), infrared (IR)], and electronic circular dichroism (ECD) calculations. Furthermore, plausible biosynthetic pathways of these compounds were deduced. The bioassay results indicated that compound 8 displayed more significant antifeedant activity against <jats:italic>S. frugiperda</jats:italic> larvae than azadirachtin and compounds 5–7 demonstrated superior insecticidal activities compared to abamectin. Moreover, comparative transcriptome analysis revealed that compound 6 exerted a distinct insecticidal mechanism. In the early stages, it regulated the expression of genes associated with peptidases and chitinases. Subsequently, this regulation influenced the expression of genes related to cuticle proteins and other relevant genes, ultimately resulting in <jats:italic>S</jats:italic>. <jats:italic>frugiperda</jats:italic> larval death. These results were further confirmed by real‐time quantitative polymerase chain reaction (RT‐qPCR) experiments.CONCLUSIONThis research will provide valuable information for the development of indole diterpenoids as novel microbial insecticides for the management of <jats:italic>S. frugiperda</jats:italic>. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"17 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon nutrition enhances CYP450s‐ and GST‐mediated metabolism of sulfosulfuron in wheat and an invasive weed, Hordeum vulgare subsp. spontaneum","authors":"Akbar Aliverdi, Ali Asghar Chitband","doi":"10.1002/ps.8818","DOIUrl":"https://doi.org/10.1002/ps.8818","url":null,"abstract":"BACKGROUNDAlthough silicon nutrition in crops has been reported to improve growth and herbicide tolerance, the response of crop‐associated weeds has not been studied.RESULTSIn glasshouse studies, co‐applying potassium silicate (K<jats:sub>2</jats:sub>SiO<jats:sub>3</jats:sub>) + silicate‐solubilizing bacteria (SSB) synergistically increased wheat and spontaneous barley's silicon content by 2.2‐ and 2.6‐fold, respectively. Based on the median effective dose, it increased wheat (2.2–2.8‐fold) and spontaneous barley (3.5–3.7‐fold) tolerance to sulfosulfuron. With/without silicon nutrition, pretreating plant species with malathion or 4‐chloro‐7‐nitrobenzofurazan made them more sensitive to sulfosulfuron. Without silicon nutrition, cytochrome P450 reductase (1.3 <jats:italic>versus</jats:italic> 0.7 U g<jats:sup>−1</jats:sup>), glutathione S‐transferase (1.7 <jats:italic>versus</jats:italic> 1.2 U g<jats:sup>−1</jats:sup>), superoxide dismutase (23.7 <jats:italic>versus</jats:italic> 14.5 U mg<jats:sup>−1</jats:sup>), and catalase (412.9 <jats:italic>versus</jats:italic> 311.5 U mg<jats:sup>−1</jats:sup>) were more active in wheat than in spontaneous barley. All enzymes became more active with silicon nutrition, the increase was higher in spontaneous barley. Field studies conducted at two locations showed that without silicon nutrition and sulfosulfuron, spontaneous barley reduced wheat grain yield by 36.8–41.5%. Without sulfosulfuron, co‐applying K<jats:sub>2</jats:sub>SiO<jats:sub>3</jats:sub> + SSB synergistically increased spontaneous barley biomass by 37.4–76.1%, reducing wheat grain yield by 59.0–62.1%. Without silicon nutrition, sulfosulfuron (40 g ha<jats:sup>−1</jats:sup>) controlled spontaneous barley by 71.8–98.5%. Furthermore, it controlled spontaneous barley by 18.7–29.9% when K<jats:sub>2</jats:sub>SiO<jats:sub>3</jats:sub> + SSB was co‐applied.CONCLUSIONSilicon nutrition made both plants more tolerant to sulfosulfuron due to the increased activity of enzymes that metabolize herbicides and scavenge reactive oxygen species. It should be avoided in spontaneous barley‐infested wheat fields because it is more beneficial for spontaneous barley and reduces sulfosulfuron efficacy. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"13 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing plant resistance to tobacco mosaic virus through the combined application of Verticillium dahliae Aspf2‐like protein and microelements","authors":"Shichen Li, Fupeng Mei, Waqar Ahmed, Tao Jiang, Linyuan Yang, Xiaodong Hu, Meiwei Zhao, Xiaoci Peng, Ren Peng, Yingfen Yang, Wei Zhang, Mingmin Li, Zhengxiong Zhao","doi":"10.1002/ps.8824","DOIUrl":"https://doi.org/10.1002/ps.8824","url":null,"abstract":"BACKGROUNDTobacco mosaic virus (TMV) poses a significant threat to global agriculture, infecting economically vital crops such as tobacco, tomato, pepper, and potato. Previous studies have suggested that the <jats:italic>Verticillium dahliae</jats:italic> Aspf2‐like protein (VDAL) enhances plant resistance to TMV. This study investigated the preventive and therapeutic effects of VDAL, with and without microelements, on TMV resistance by analyzing plant hormone levels, defense related enzyme activities, and transcriptomic responses.RESULTSPlants were subjected to six experimental treatments: CK0 (untreated control, no TMV or VDAL treatment), CK (TMV inoculated control), T1 (preventive VDAL treatment), T2 (preventive VDAL + microelements), CT1 (therapeutic VDAL treatment), and CT2 (therapeutic VDAL + microelements). TMV inoculation (CK) significantly increased (<jats:italic>P</jats:italic> &lt; 0.05) TMV content, jasmonic acid (JA), salicylic acid (SA) levels, and activities of defense related enzymes, including benzoic acid 2‐hydroxylase (BA2H), peroxidase (POD), polyphenol oxidase (PPO), and superoxide dismutase (SOD), compared to CK0. Both preventive treatments (T1 and T2) effectively reduced TMV content and enhanced JA, SA, and defense related enzyme activities. Notably, the microelement‐supplemented preventive treatment (T2) showed 37.73% greater reduction in TMV content compared to T1. Similarly, the therapeutic applications, CT2 reduced the TMV content by 32.50% than CT1. Treatments T2 and CT2 also increased the contents of JA by 5.48% and 2.88%, respectively compared to their respective controls. Transcriptomic analysis revealed that these treatments activated plant–pathogen interaction pathways and pathogen‐associated molecular pattern‐triggered immunity (<jats:italic>PTI</jats:italic>), with significant upregulation of key defense related genes (e.g., <jats:italic>CALM</jats:italic>, <jats:italic>BAK1</jats:italic>, <jats:italic>PTI6</jats:italic>, and <jats:italic>WRKY33</jats:italic>), indicating a robust antiviral defense response.CONCLUSIONOverall, we conclude that the synergistic application of VDAL and microelements significantly enhances plant resistance to TMV through coordinated activation of phytohormone signaling, defense enzymes, and immune‐related gene expression. This combined approach offers an effective, eco‐friendly alternative for sustainable management of viral diseases in agricultural crops. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"33 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}