Pest Management Science最新文献

{"title":"Field-realistic exposure to pesticide residues in beehive pollen and honey and the associated risk to honeybees.","authors":"Xingyi Zhu,Bangyan Song,Haiyan Zhang,Yujun Long,Yao Dan,Guohui Liao,Minggui Chen,Lixin Qin,Xiangyan Chen,Feng Zhu","doi":"10.1002/ps.70264","DOIUrl":"https://doi.org/10.1002/ps.70264","url":null,"abstract":"BACKGROUNDHoneybees, as the most important pollinators, can be affected by pesticides while foraging for nectar and pollen. Previous studies have shown that some pesticides can be found in pollen and nectar in realistic samples. However, the actual exposure characteristics of bees and the core sources of pesticides in the karst landform of Guizhou remain unknown at present. In this study, environmental DNA analysis was performed on bee pollen to examine its plant sources. Furthermore, the types and concentrations of pesticides in honey and bee pollen to which the associated bees were exposed were determined using the optimized QuEChERS extraction method combined with ultraperformance liquid chromatography-tandem mass spectrometry. In addition, the risks posed by the detected pesticides to the bees were assessed.RESULTSResults showed that spring beehive pollen mainly originated from agricultural crops, while autumn beehive pollen primarily originated from wild plants. Among the 427 samples of honey and bee pollen. Twenty-five types of pesticides were identified, including thiamethoxam, difenoconazole, and chlorantraniliprole, which were the most frequently detected pesticides in the samples. The overall pesticide detection rate for the samples was 54.8%. The HQ and ΣHQ values of the detected pesticides for bees were less than 1.0.CONCLUSIONA higher number of pesticides was detected in the spring samples (24 pesticides) than in the autumn samples (seven pesticides), implying that the bees encounter greater challenges in spring and summer than in autumn and winter. The residual levels of these pesticides posed acceptable exposure risk levels to the bees. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"103 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NanoClay-enhanced spray-induced gene silencing as a biological control strategy for Rehmannia glutinosa root rot disease.","authors":"Bingyang Guo,Yanhui Yang,Jiayi Tan,Rong Wang,Tianrui Xue,Mingjie Li,Zhongyi Zhang","doi":"10.1002/ps.70261","DOIUrl":"https://doi.org/10.1002/ps.70261","url":null,"abstract":"BACKGROUNDRehmannia glutinosa, a herb of significant medicinal and food value, is highly susceptible to pathogen infections. Fusarium oxysporum f.sp. R. glutinosa (FORg), a pathogenic strain isolated from R. glutinosa, induces root rot disease, leading to severe yield losses. Currently, resistant cultivars and effective control strategies for R. glutinosa root rot remain very limited. This study aims to investigate the feasibility and potential of SIGS technology in preventing and controlling R. glutinosa root rot caused by FORg.RESULTSThis study demonstrates that FORg hyphae can capably take up dsRNA, confirming their suitability for SIGS-based disease control. dsRNA targeting SGE1 and CYP51, synthesized in vivo and extracted through low-cost ethanol method, significantly suppressed the expression of virulence-associated genes in both hyphae and conidia. Root disc assays further validated that exogenous dsRNA effectively attenuated FORg pathogenicity. Combined applications of SGE1 and CYP51S dsRNAs synergistically reduced disease severity compared to single-target treatments. Additionally, dsRNA encapsulated in layered double hydroxide (LDH) prolonged functional durability, with sustained plant protection observed for 15 days post-spraying, outperforming unencapsulated dsRNA.CONCLUSIONThis finding establishes the feasibility of SIGS for controlling FORg-induced root rot in R. glutinosa and highlights the potential of nanomaterial encapsulation in addressing persistent fungal epidemics in agricultural systems. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"18 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to benzaldehyde and larval feeding experiences modulate olfactory and oviposition behaviors in female Grapholita molesta (Lepidoptera: Tortricidae).","authors":"Yi Wang,Wei-Na Kong,Rui-Wei Shen,Hang Chen,Yu-Song Zhao,Jie Li,Rui-Yan Ma","doi":"10.1002/ps.70245","DOIUrl":"https://doi.org/10.1002/ps.70245","url":null,"abstract":"BACKGROUNDGrapholita molesta is an important pest of rosaceous fruit trees. Female oviposition affects larval infestation levels in orchards. This behavior, shaped by larval feeding experiences and female olfactory responses, points to an experience-modulated semiochemical strategy based on host plant secondary metabolites. To elucidate the combined effects of non-volatile and volatile components on female oviposition, we analyzed the volatile profiles of peach, pear, and apple shoots/fruits under intact, mechanically injured, and larval damage states throughout the growing season, along with female oviposition preferences, and compared olfactory responses (electroantennogram (EAG) and wind-tunnel experiments) and oviposition bioassays with benzaldehyde (amygdalin hydrolysate) between females with two larval amygdalin-feeding experiences.RESULTSFemale oviposition preference and benzaldehyde emissions, both modulated by host factors, exhibited synchronous fluctuations. In EAG and wind-tunnel experiments, females not fed amygdalin during larval stages exhibited a strong attraction to benzaldehyde at concentrations of 10 and 0.1 μg/μL; however, females from larvae fed amygdalin showed a weaker long-distance orientation but greater responses to benzaldehyde during close-range approach and landing behaviors. Females fed amygdalin during larval stages were more likely to be responsive to lower concentrations of benzaldehyde (0.01, 0.1, and 1.0 μg/μL) for oviposition. In contrast, close-range attraction and oviposition behaviors were stronger for females that developed from amygdalin-fed larvae than those that were not fed amygdalin.CONCLUSIONDietary exposure to amygdalin during the larval stages increased the olfactory responses of females to benzaldehyde. Female oviposition behavior is co-regulated by larval experience derived from amygdalin ingestion and benzaldehyde as a host semiochemical signal. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"65 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility to cyflumetofen in populations of the citrus leprosis mite, Brevipalpus yothersi, from Brazilian citrus orchards.","authors":"Hector Alonso Escobar-Garcia,Daniel Júnior de Andrade","doi":"10.1002/ps.70262","DOIUrl":"https://doi.org/10.1002/ps.70262","url":null,"abstract":"BACKGROUNDBrevipalpus yothersi Baker (Acari: Tenuipalpidae), the citrus leprosis mite, is currently the most economically significant mite pest in commercial citrus orchards, serving as the vector of citrus leprosis virus C (CiLV-C, Cilevirus leprosis). Its control has traditionally relied on the intensive use of synthetic acaricides, raising concerns regarding field efficacy and resistance development. To support integrated pest management (IPM) and resistance management strategies, monitoring the susceptibility of field populations is essential. Cyflumetofen, an acaricide that inhibits mitochondrial complex II electron transport, offers a distinct mode-of-action (MoA) for mite control. This study aimed to characterize the susceptibility of B. yothersi populations to cyflumetofen to evaluate its potential use in sustainable resistance management and prolong its efficacy in commercial applications.RESULTSField populations exhibited high susceptibility to cyflumetofen, with median lethal concentration (LC50LC₅₀) values ranging from 0.36 to 1.31 mg L-1 and 95% lethality (LC95) values from 1.22 to 4.99 mg L-1. Resistance levels were low, with resistance ratios below 2.79-fold at LC50 and below 3.06-fold at LC95, relative to a susceptible reference population.CONCLUSIONCyflumetofen proved effective against B. yothersi in the surveyed regions, with field populations showing high susceptibility. To maintain its efficacy and extend its commercial longevity, rotation with other synthetic acaricides featuring different MoAs is recommended as part of a resistance management plan. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"20 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genomic analysis and functional characterization of a new entomopathogenic strain, Bacillus thuringiensis subsp. israelensis VCRC B646, antagonistic to mosquito vectors (Diptera: Culicidae), isolated from soil in Tamil Nadu, India.","authors":"Abhisubesh Vijayakumar,Mathivanan Ashokumar,Bhavna Gupta,Sahadiya Mandodan,Jibi Lukose,Aneha Kunnikuruvan,Kakhuangailiu Gangmei,Bhagyashree Bora,Hemaladkshmi Padmanaban,Subbiah Poopathi","doi":"10.1002/ps.70236","DOIUrl":"https://doi.org/10.1002/ps.70236","url":null,"abstract":"BACKGROUNDMosquito-borne diseases such as malaria, dengue and filariasis remain major public health threats, demanding effective and environmentally-safe control measures. Bacillus thuringiensis subsp. israelensis (Bti) is widely employed as a biopesticide owing to its Cry and Cyt δ-endotoxins. Continuous isolation and genomic characterization of potent indigenous strains are crucial for strengthening vector control strategies. This study aimed to assess the larvicidal efficacy, genomic features, plasmid architecture and environmental safety of a novel Indian isolate, Bti VCRC B646.RESULTSBti VCRC B646 (JBAMKE010000000) exhibited potent larvicidal activity against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus, with median lethal concentrations (LC₅₀) values of 0.0067, 0.0085 and 0.0072 μg mL-1, respectively, and LC₉₀ values of 0.0112, 0.0146 and 0.0129 μg mL-1. Whole-genome sequencing revealed a 6.6-Mbp genome (34.83% GC) comprising a chromosome and seven plasmids, including one harboring insecticidal toxin genes (Cry4A, Cry4B, Cry11A, Cry10Aa, Cry15A, Cyt1, Cyt2). Comparative genomics (ANI, dDDH, phylogeny) confirmed its identity as Bti, closely related to the WHO-reference strain Bti AM65-52. SDS-PAGE and toxin gene mining validated δ-endotoxins and accessory chitinases and metalloproteases. Secondary metabolite biosynthetic clusters, phage elements and mobile genetic elements were identified, whereas no major human pathogenic markers were detected. Nontarget aquatic organisms remained unaffected at ≤30-fold LC₉₀ doses.CONCLUSIONSBti VCRC B646 demonstrates exceptional larvicidal potency and genomic richness, with plasmid-borne δ-endotoxins and biosynthetic versatility. Its strong efficacy, safety profile and ecological adaptability underscore its potential as a next-generation, sustainable microbial larvicide for integrated vector management. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"4 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of lithium chloride on queen egg-laying performance and worker honey bee behavior.","authors":"Sevin Sedat,Jakob Avi Shimshoni,Afik Ohad,Zarhin Shlomo,Hagai Yehoshua Shpigler","doi":"10.1002/ps.70253","DOIUrl":"https://doi.org/10.1002/ps.70253","url":null,"abstract":"BACKGROUNDLithium chloride (LiCl) has recently emerged as a potential treatment for Varroa in honey bee colonies, yet its effects on bee behavior remain poorly understood. This study investigates, for the first time, the impact of oral exposure to chronic 50 mM LiCl-administered via candy over 7-10 days-on three key behavioral traits: queen oviposition, worker brood care, and worker aggression. Laboratory assays were conducted using caged bees with ad libitum access to LiCl-enriched or control candy.RESULTSEgg-laying rates did not differ significantly between LiCl-treated and control groups. Worker bees exposed to LiCl showed a significant increase in mite mortality. Brood care behavior, assessed using a 4-day-old queen larva, was unaffected in terms of nursing frequency and duration. However, LiCl-treated workers exhibited a significant reduction in aggressive behaviors compared to controls.CONCLUSIONSLiCl did not adversely influence queen egg laying or workers' nursing behavior, reinforcing previous findings suggesting negligible long-term risks to essential colony maintenance activities such as queen reproductive performance and nursing behavior. The reduced aggression observation is consistent with reports from other species, suggesting a conserved modulatory role of Li on aggression across the animal kingdom. From an applied perspective, these results imply that LiCl can be used as Varroa control without compromise on colony reproduction and brood care. Furthermore, LiCl could potentially facilitate the management of aggressive colonies, thereby reducing stress for both the bees and their handlers. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"62 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145153458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The entomopathogenic fungus Beauveria bassiana employs heterokaryon incompatibility genes in its competitive mechanism against cuticular yeasts of brown planthoppers.","authors":"Hang-Rong Xu,Jia-Peng Yang,Qian-Xuan Yu,Hai-Jun Xu,Ming-Guang Feng,Sheng-Hua Ying","doi":"10.1002/ps.70269","DOIUrl":"https://doi.org/10.1002/ps.70269","url":null,"abstract":"BACKGROUNDEntomopathogenic fungi (EPF) develop antagonistic strategies to compete with bacteria on the host cuticle surface, facilitating fungal invasion of their host. However, the presence of fungal species on host cuticles and the mechanisms by which EPF compete against cuticular fungi remain unclear.RESULTSBeauveria bassiana is a ubiquitous entomopathogenic fungus with significant potential for biological control of the brown planthopper Nilaparvata lugens. In this study, we demonstrated that three yeast species are dominant on the cuticle surface of N. lugens: Kurtzmaniella quercitrusa, Vanrija humicola and Candida intermedia. These yeasts exhibited significant inhibitory effects on conidial germination but not on blastospore germination. Using K. quercitrusa as a representative species, insect bioassays indicated that conidial virulence was significantly weakened by cuticular yeast compared with blastospores. Notably, the heterokaryon incompatibility gene BbHET-C2 was significantly involved in the resistance of B. bassiana conidia to yeast inhibition. BbHET-C2 played a more significant role in fungal virulence caused by blastospores than by conidia when B. bassiana competed with cuticular yeasts.CONCLUSIONSThe current findings reveal that yeasts are dominant on the cuticles of planthoppers and suggest that EPF blastospores are a promising infectious inoculum, with the advantage of competing against fungal defenders in the hosts. These findings provide novel strategies for the development of formulations and strain improvement of insect pathogenic fungi. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"154 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145153459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA interference-mediated silencing of hypoxia-inducible factor 1 signaling pathway genes enhanced the hypoxia sensitivity in the brown-legged grain mite, Aleuroglyphus ovatus.","authors":"Yang Zou,Hui Ai,Gang Miao,Yudie Hu,Hejun Liu,Bin Xia,Tianrong Xin,Zhiwen Zou","doi":"10.1002/ps.70231","DOIUrl":"https://doi.org/10.1002/ps.70231","url":null,"abstract":"BACKGROUNDThe allergen and storage pest Aleuroglyphus ovatus (Troupeau, 1878) threatens human health and food safety. While modified atmosphere shows pest control potential, its efficacy against this species remains unverified. This study evaluates nitrogen-induced hypoxia effects on A. ovatus mortality, development, and hypoxia-response mechanisms to establish a scientific basis for targeted control strategies.RESULTSA. ovatus mortality increased with prolonged hypoxia exposure and reduced oxygen concentrations. Following 1-day hypoxia treatment, hatchability declined to 60.85% of control levels under 2% O2, whereas it remained stable at 5% O2. Developmental delays of 2.51 days (5% O2) and 3.12 days (2% O2) were observed in total immature stages compared to normoxic controls. The hypoxia-inducible factor 1 (HIF1) signaling pathway-comprising HIF1α, HIF1β, and PHD-demonstrated evolutionarily conserved functionality, with synchronous expression patterns across all three genes suggesting feedback-mediated co-regulation. Crucially, RNA interference respectively targeting all three genes enhanced adult mortality, achieving 1.67-2.06-fold and 2.19-2.88-fold increases at 5% and 2% O2, compared to non-interference groups. Interference targeting HIF1α in eggs also resulted in suppressed embryonic development and hatching.CONCLUSIONMortality acceleration, developmental delays, and pathway co-regulation under hypoxia expose vulnerabilities of A. ovatus exploitable for pest management. Data confirm that combining atmospheric controls with single-gene RNAi targeting any of these three genes enhances lethality. As a systematic dissection of the HIF1 signaling pathway's hypoxic response in arthropods-a rarely explored area-this work established an interdisciplinary strategy integrating ecological interventions and molecular silencing, providing a theoretical basis for sustainable pest control. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"194 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of resistance to dsRNA in the wheat aphid (Schizaphis graminum) without detectable fitness trade-offs.","authors":"Kaleem Tariq,Abdul Haseeb,Ihsan Ul Haq,Juan Luis Jurat-Fuentes,Farman Ullah","doi":"10.1002/ps.70260","DOIUrl":"https://doi.org/10.1002/ps.70260","url":null,"abstract":"BACKGROUNDRNA-based insecticides utilize a double-stranded RNA (dsRNA) as an active ingredient to initiate the RNA interference (RNAi) pathway and silence the expression of a vital gene in the target insect. Despite the strong commercial interest in RNA insecticides as both insecticidal traits in transgenic crops and sprayable products, information on resistance to dsRNA is limited to coleopteran species in the Chrysomelidae family. In contrast, the resistance potential in other insect groups remains largely unknown. This study evaluated the capacity of the wheat aphid (Schizaphis graminum), a polyphagous insect known for its adaptability to insecticides, to evolve resistance to a dsRNA targeting the C002 gene (dsC002). The C002 gene was selected as the target due to its essential role in aphid feeding and the strong association between its knockdown and high aphid mortality. Additionally, we assessed through life table parameters whether resistance to dsRNA in S. graminum incurs any fitness costs.RESULTSAfter ten generations of selection using dsC002 at the half-maximal lethal concentration (LC50) for each generation, the selected S. graminum population exhibited 74.9-fold resistance compared to the parental population. No significant differences in life-history traits were observed between dsRNA-resistant and susceptible S. graminum populations, indicating that dsRNA resistance is not associated with fitness costs under laboratory conditions.CONCLUSIONSchizaphis graminum developed high levels of resistance to a dsRNA after only ten generations of selection. Resistance is not associated with fitness costs under laboratory conditions. These findings offer valuable insights for consideration and evaluation before commercial development of RNA aphidicidal products. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"1 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target discovery of inhibitors based on a G-quadruplex structure in the potato virus Y genome.","authors":"Chao Gao,Yang Xu,Tao Wang,Yihan Zou,Congbao Xie,Zhangqian Wang,Yi He,Dehua Luo","doi":"10.1002/ps.70242","DOIUrl":"https://doi.org/10.1002/ps.70242","url":null,"abstract":"BACKGROUNDPotato virus Y (PVY) exhibits a wide host range and significantly affects crop growth. Development of novel targets is an important way to discover highly active PVY inhibitors with novel structure. G-quadruplex (G4) structures formed by guanine-rich nucleic acid sequences, have emerged as validated therapeutic targets driving the development of novel antiviral and antitumor agents. The untranslated regions is a regulatory region for mRNA expression, and the G4s in this region can regulate gene expression.RESULTSTen G4 structures were identified in the genome of PVY, one of which is located in the 3'-untranslated regions (PQS10) and has the putative function of regulating gene expression. Further studies revealed that PQS10 may be folded into the dimeric G4 with two planar G-quartets. The binding affinity for G-quadruplex-ligands to PQS10 was evaluated by isothermal titration calorimetry, and the results indicated that BRACO-19, RHPS4, and TMPyP4 exhibited a higher binding affinity with PQS10. BRACO-19, RHPS4, and TMPyP4 were found to stabilize PQS10 G4 structure, thereby enhancing the inhibitory effect of PQS10 on expression of the dual luciferase reporter gene. These three compounds were found to interact with PQS10 through intercalation into its lateral external loops, and significantly inhibit the expression of the PVY gene with more than 75% inhibition rate.CONCLUSIONThis study demonstrated that G4 structures in the PVY genome are essential regulatory elements for viral proliferation, and provided new strategies for suppressing PVY proliferation. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"64 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}