Pest Management Science最新文献

{"title":" Albifimbria verrucaria DY01 : a tomato‐safe mycoherbicide candidate with broad‐spectrum activity against farmland weeds and invasive Ageratina adenophora ","authors":"Xinlin Wang, Ying Deng, Xinyi He, Huanhuan Shao, Shuyue Yu, Jinya Zhang, Bin Yong, Xiang Tao","doi":"10.1002/ps.71411","DOIUrl":"https://doi.org/10.1002/ps.71411","url":null,"abstract":"The rapid evolution of herbicide‐resistant weeds has driven urgent demand for eco‐friendly bioherbicides, yet most microbial agents suffer narrow application ranges and unstable field efficacy. In this study, an <jats:italic>Albifimbria verrucaria</jats:italic> strain DY01 was isolated from naturally diseased leaves of <jats:italic>Oplismenus undulatifolius</jats:italic> . At 4 × 10 <jats:sup>7</jats:sup> CFU mL <jats:sup>−1</jats:sup> , DY01 spore suspension caused severe chlorosis and seedling mortality across six out of seven tested weeds within 5 days, while only <jats:italic>Cyperus iria</jats:italic> sustained minimal injury. The mortality rates of <jats:italic>Echinochloa crus‐galli</jats:italic> , <jats:italic>Microstegium vimineum</jats:italic> , and the invasive species <jats:italic>Ageratina adenophora</jats:italic> reached 97.84%, 100%, and 100%, respectively. Crop safety evaluation further demonstrated unique selectivity: tomato showed no phytotoxic symptoms even at the maximum test concentration, whereas Chinese cabbage suffered near‐complete mortality, wheat displayed high lethal rates, and maize plus broad bean developed extensive necrotic lesions and growth retardation. Comparative bioassays confirmed that the &lt;1000 Da dialyzed fraction exhibited stronger weed‐suppressive effects than crude extract, indicating that active metabolites are small‐molecule‐weight compounds. The genome of DY01 was sequenced, yielding a 44.73 Mb assembly comprising 10 scaffolds with 13 600 predicted genes and 92 biosynthetic regions were identified, including T1PKS, NRPS, and terpene‐precursor clusters, indicating the substantial secondary‐metabolite biosynthetic potential of DY01. This study identified a broad‐spectrum, tomato‐safe <jats:italic>A. verrucaria</jats:italic> isolate with potent phytotoxicity, offering a valuable microbial resource and molecular foundation for developing targeted bioherbicides applied in tomato planting systems. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"81 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of salicylic acid methyltransferase ( ZmSAMT ) enhances resistance to Rhopalosiphum padi in maize","authors":"Yingyue Xu, Yan Wang, Mingxuan Sui, Yunpeng Zhao, Shuai Liu, Haibin Yuan","doi":"10.1002/ps.71420","DOIUrl":"https://doi.org/10.1002/ps.71420","url":null,"abstract":"BACKGROUND Aphids are important pests of maize, including Bt‐transgenic maize, and their effective management remains a major challenge. Herbivore‐induced plant volatiles (HIPVs), particularly methyl salicylate (MeSA), play a critical role in mediating tritrophic interactions and plant defense in agroecosystems. Exogenous application of synthetic MeSA can be effective against aphids, however, its pronounced volatility and maintenance costs limit large‐scale agricultural applications. RESULTS In this study, we generated <jats:italic>ZmSAMT</jats:italic> ‐overexpressing transgenic maize to enable continuous endogenous MeSA emission, establishing a sustainable strategy for aphid management. <jats:italic>In vitro</jats:italic> enzymatic assays confirmed that ZmSAMT specifically methylated salicylic acid (SA) to form MeSA. Notably, aphid infestation triggered significant upregulation of <jats:italic>ZmSAMT</jats:italic> expression in wild‐type maize. Behavioral assays demonstrated that elevated MeSA emission in transgenic line OE3 significantly repelled <jats:italic>Rhopalosiphum padi</jats:italic> and actively recruited the predator ladybeetle <jats:italic>Harmonia axyridis</jats:italic> . Independent of olfactory‐mediated effects, aphids feeding on OE3 plants exhibited prolonged developmental duration, a shortened reproductive period, and reduced total fecundity. Furthermore, we observed a significant airborne defense effect that wild type maize situated adjacent to the transgenic plants OE3 harbored fewer aphids. CONCLUSION Taken together, our studies indicate that <jats:italic>ZmSAMT</jats:italic> represents a highly valuable genetic resource for breeding aphid‐resistant maize varieties and developing green pest control strategies. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"234 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxathiazepane compounds as potential insecticidal agents","authors":"Nataly E. Gruntenko, Kseniya S. Kovaleva, Evgenia K. Karpova, Natalya V. Adonyeva, Sofia N. Sharmankina, Vladislav V. Oreshko, Elena V. Burdina, Maksim A. Deryuzhenko, Anna S. Khotskina, Olga I. Yarovaya","doi":"10.1002/ps.71403","DOIUrl":"https://doi.org/10.1002/ps.71403","url":null,"abstract":"BACKGROUND The spread of insecticide resistance among major agricultural and vector pests necessitates the discovery of compounds with novel modes of action and favorable safety profiles. 1,5,3‐Oxathiazepanes represent a previously unexplored class of heterocycles. This study aimed to evaluate the insecticidal potential of ten 1,5,3‐oxathiazepane derivatives and assesses their toxicity in mammals and human cells. RESULTS Screening against <jats:italic>Drosophila melanogaster</jats:italic> adults identified aliphatic‐substituted derivatives (compounds 1 – 6 ) as active, with compounds 1 – 4 causing 100% mortality at 1% within 48 h. Aromatic or heteroaromatic substituents led to a complete loss of activity. Compound 3 (3‐hexyl‐1,5,3‐oxathiazepane) showed concentration‐dependent insecticidal effects, with 24 h median lethal concentration (LC <jats:sub>50</jats:sub> ) of 0.77% (7.7 g L <jats:sup>−1</jats:sup> ) via feeding and 24‐h median lethal dose (LD <jats:sub>50</jats:sub> ) of 3.1 μg per fly via topical application. At 3% (30 g L <jats:sup>−1</jats:sup> ), compound 3 killed 100% of <jats:italic>Tenebrio molitor</jats:italic> and <jats:italic>Oxyhaloa deusta</jats:italic> within 24 h. Acute oral LD <jats:sub>50</jats:sub> in mice was 1098 mg kg <jats:sup>−1</jats:sup> (hazard category 4). CONCLUSION This is the first report of insecticidal activity in 1,5,3‐oxathiazepanes. Compound 3 exhibits broad‐spectrum activity across multiple insect orders and low mammalian toxicity, combined with a scalable one‐pot synthesis. These findings establish oxathiazepanes as a new chemical scaffold with interesting insecticidal activity and a favorable safety profile against mammals, which warrants further investigation to determine its mode of action and potential for managing resistance. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"13 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering citrus for psyllid control: virus‐induced silencing of osmoregulatory genes reduces survival and development of Diaphorina citri","authors":"Nabil Killiny, Mahnaz Rashidi, Yagyaraj Joshi, Turksen Shilts, Lamiaa M Mahmoud, Manjul Dutt, Lukasz L Stelinski, Bryony C Bonning","doi":"10.1002/ps.71407","DOIUrl":"https://doi.org/10.1002/ps.71407","url":null,"abstract":"BACKGROUND The Asian citrus psyllid ( <jats:italic>Diaphorina citri</jats:italic> ) is the insect vector of ‘ <jats:italic>Candidatus</jats:italic> Liberibacter asiaticus’, the causal agent of huanglongbing (HLB), the most destructive citrus disease worldwide. Current management depends largely on repeated insecticide applications, underscoring the need for sustainable alternatives. Osmoregulation is indispensable for <jats:italic>D</jats:italic> . <jats:italic>citri</jats:italic> because the insect feeds exclusively on sucrose‐rich phloem sap and must continuously regulate carbohydrate metabolism and water balance to maintain physiological homeostasis. RNA interference (RNAi)‐mediated silencing of sucrose hydrolase ( <jats:italic>DcSuh</jats:italic> ) and aquaporin ( <jats:italic>DcAQP</jats:italic> ), two key components of the psyllid osmoregulatory network, significantly reduces psyllid survival. However, efficient and durable delivery systems capable of sustaining RNAi under natural plant‐feeding conditions remain limited. RESULTS We developed recombinant Citrus tristeza virus (CTV) vectors that express double‐stranded RNA (dsRNA) targeting <jats:italic>DcSuh</jats:italic> and <jats:italic>DcAQP</jats:italic> , enabling continuous citrus‐mediated RNAi. <jats:italic>D</jats:italic> . <jats:italic>citri</jats:italic> reared on citrus plants infected with these engineered CTV vectors were evaluated over two successive generations to determine the long‐term effects of gene silencing on insect performance. Silencing either target gene significantly reduced psyllid fitness by decreasing survival, longevity, and fecundity while increasing developmental abnormalities and mortality. These effects persisted in the second generation, demonstrating sustained RNAi activity. CONCLUSION This study demonstrates that CTV is an efficient and durable vehicle for plant‐mediated RNAi in citrus and identifies osmoregulation as a highly susceptible physiological target for management of <jats:italic>D</jats:italic> . <jats:italic>citri</jats:italic> . Integrating virus‐mediated dsRNA production with essential insect physiological targets offers a promising strategy for long‐term suppression of psyllid populations and a sustainable approach to reducing the spread of huanglongbing. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"8 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149221436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating niches of the pathogen, multiple host and vector species highlights abiotic constraints and host-vector interactions shaping pine wilt disease risk in China.","authors":"Wenquan Wang, Ting Du, Jiangxia Ye, Xiaohong Huang, Yangyang Li, Zhonghe Zhang, Lei Shi","doi":"10.1002/ps.71295","DOIUrl":"https://doi.org/10.1002/ps.71295","url":null,"abstract":"<p><strong>Background: </strong>Pine wilt disease (PWD), caused by Bursaphelenchus xylophilus (PWN), is a major threat to forest health and pest management in China. Because PWD results from interactions among the pathogen, vector insects, and host plants, single-component analyses may misrepresent disease risk and overlook species-specific contributions. We integrated the environmental niches of the pathogen, hosts, and vectors to characterize abiotic constraints and biotic influences on PWD risk, quantify species-specific contributions and interactions, and inform surveillance priorities.</p><p><strong>Results: </strong>Ensemble ecological niche models were developed for PWN, 17 susceptible hosts, and 10 potential cerambycid vectors across China and integrated with GeoDetector analysis. Although suitability patterns were broadly consistent, PWN had a much narrower suitable range than hosts and vectors, and this concordance declined under future climate scenarios. Bioclimatic variables were the primary determinants of habitat suitability for most species, whereas PWN showed narrower optimal ranges for key environmental drivers than most hosts and vectors. GeoDetector revealed substantial interspecific variation in explanatory power for PWN suitability, with Pinus massoniana, P. thunbergii, and Monochamus alternatus showing the highest q-values (0.78, 0.77, and 0.79, respectively). All host-vector combinations explained PWN suitability better than either species alone.</p><p><strong>Conclusion: </strong>PWD risk in China cannot be inferred from host or vector availability alone, but is shaped by stronger abiotic constraints on PWN and host-vector interactions. Climatically marginal regions with suitable hosts and vectors should be prioritized for surveillance, and species with high q-values warrant further investigation. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and molluscicidal activity of a triazolopyrimidine derivative against Monacha cartusiana ","authors":"Radwa M. Said, Doaa A. Elsayed, Wesam S. Shehab, Gehan T. El‐Bassyouni, Sahar M. Mousa, Yasmin A. Abd El‐Latif","doi":"10.1002/ps.71397","DOIUrl":"https://doi.org/10.1002/ps.71397","url":null,"abstract":"BACKGROUND One of the common and harmful pests in Egypt's agroecosystems is <jats:italic>Monacha cartusiana</jats:italic> , highlighting the need for the development of novel and effective molluscicidal agents for improved control. RESULTS In this study, a novel triazolopyrimidine derivative was prepared using manganese(II,III) oxide (Mn <jats:sub>3</jats:sub> O <jats:sub>4</jats:sub> ) nanoparticles as a heterogeneous catalyst. The synthetic compound showed poor cytotoxicity against HFB4 cells and inhibited CACO‐2 cell growth. It also showed marked antioxidant effects against 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and ferric‐reducing antioxidant power (FRAP). Mortality rates of adult and juvenile snails increased with compound concentration and exposure time. For adults, lethal concentrations at 50% (LC <jats:sub>50</jats:sub> ), 25% (LC <jats:sub>25</jats:sub> ), and 10% (LC <jats:sub>10</jats:sub> ) were 0.51%, 0.1275%, and 0.051%, respectively; for juveniles, they were 0.032%, 0.008%, and 0.0032%, respectively. The total hemocyte count of adult snails significantly decreased after 28 days of exposure to LC <jats:sub>25</jats:sub> and LC <jats:sub>10</jats:sub> . Phenoloxidase levels in soft tissue significantly decreased. For antioxidant biomarkers, superoxide dismutase (SOD) significantly decreased, while malondialdehyde (MDA) and glutathione <jats:italic>S</jats:italic> ‐transferase (GST) significantly increased. After exposure to LC <jats:sub>10</jats:sub> , mild histological abnormalities were observed in digestive gland sections, including vacuolation and necrosis of epithelial cells in some tubule regions; after exposure to LC <jats:sub>25</jats:sub> , serious histological abnormalities, including necrosis of hepatopancreas tubules, were conspicuous. Transmission electron microscopy (TEM) showed many ultrastructural abnormalities in the digestive gland of treated snails. CONCLUSION These findings indicate that the synthetic compound exhibits promising molluscicidal activity against <jats:italic>M. cartusiana</jats:italic> , making it the key result and a potential candidate for further investigation as a molluscicidal agent. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"293 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149172918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celastrol as a promising botanical antifungal agent against sorghum anthracnose: efficacy and synergistic potential","authors":"Ling Zhang, Yue Ding, He‐Yu Huang, Ke Yang, Gao‐Ming Hu, Xiang Zhou, Song Yang","doi":"10.1002/ps.71381","DOIUrl":"https://doi.org/10.1002/ps.71381","url":null,"abstract":"BACKGROUND Sorghum is an essential staple crop across the globe, but its productivity is persistently threatened by <jats:italic>Colletotrichum sublineola</jats:italic> , the causal agent of sorghum anthracnose. This destructive fungal disease severely impairs yield stability and seed quality. Despite ongoing research efforts, considerable gaps remain in the exploration of natural product resources for the development of dedicated botanical fungicides targeting sorghum anthracnose. RESULTS In order to address this research gap, we adopted a natural product repurposing strategy to systematically screen 60 commercially available natural products and evaluate their <jats:italic>in vitro</jats:italic> antifungal efficacy against <jats:italic>C. sublineola</jats:italic> . Here, we report for the first time that celastrol exhibits potent <jats:italic>in vitro</jats:italic> inhibition of <jats:italic>C. sublineola</jats:italic> , with a median effective concentration (EC <jats:sub>50</jats:sub> ) value of 1.24 μg mL <jats:sup>–1</jats:sup> . <jats:italic>In vivo</jats:italic> assays confirmed that celastrol exhibited prominent curative and protective effects against sorghum anthracnose. Mechanistic analyses revealed that celastrol induces excessive intracellular reactive oxygen species accumulation, reduces mitochondrial membrane potential, and depletes cellular ATP content. These physiological perturbations disrupt fungal plasma membrane integrity and ultimately inhibit fungal proliferation. Furthermore, celastrol exhibited no obvious effects on seed germination at 200 μg mL <jats:sup>–1</jats:sup> . Additionally, combination treatment with rationally optimized ratios of celastrol and azoxystrobin also produced significant synergistic antifungal activity. CONCLUSIONS Collectively, our results demonstrate the robust antifungal activity of celastrol against <jats:italic>C. sublineola</jats:italic> , highlighting its potential for development as both a standalone botanical antifungal agent and a key synergistic component in fungicide mixtures. Our findings provide a promising candidate for the sustainable management of sorghum anthracnose. © 2026 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"68 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149172870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined effects of canola plant density and insecticide management strategies on flea beetle abundance, canola defoliation, and yield across the Canadian prairies","authors":"Shayla Woodland,&nbsp;Héctor A Cárcamo,&nbsp;Tyler Wist,&nbsp;Jennifer Otani,&nbsp;John Gavloski,&nbsp;Rob W Duncan,&nbsp;Tharshinidevy Nagalingam,&nbsp;Alejandro C Costamagna","doi":"10.1002/ps.70320","DOIUrl":"10.1002/ps.70320","url":null,"abstract":"<p>The crucifer flea beetle, <i>Phyllotreta cruciferae</i> (Goeze), and the striped flea beetle, <i>Phyllotreta striolata</i> (Fabricius) (Coleoptera: Chrysomelidae), are devastating pests of canola (<i>Brassica napus</i> (L.)) in North America. Currently, farmers rely on prophylactic, neonicotinoid insecticide-coated seed to control them. This results in most fields being treated with insecticides regardless of flea beetle levels. Moreover, if emergence of canola or seedling growth is delayed, or under very high flea beetle populations, seed treatments alone can fail to suppress damage and require foliar insecticides or reseeding. We conducted 15 replicated field trials in four regions of the Canadian prairies testing the effects of three canola planting densities combined with two flea beetle management treatments (seed treatment and foliar spray) and two controls (‘flea beetle-free’ treatment and untreated control) from 2018 to 2021. Although flea beetles increased as plant density increased, as predicted by the resource concentration hypothesis, this was offset by a dilution at the plant level and critically, defoliation levels did not increase at high plant densities. Using seed treatments as a management strategy generally produced similar results to using only foliar sprays at the 25% injury threshold when considering flea beetles per plant and yield. More importantly, yield increased with increased plant density regardless of flea beetle abundance, management treatment, and region. We conclude that increasing plant density is a sustainable technique that may be combined with other strategies to protect canola yield, but finding an economical plant density to protect yield from flea beetle damage will require further research. © 2025 His Majesty the King in Right of Canada and The Author(s). <i>Pest Management Science</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry. Reproduced with the permission of the Minister of Agriculture and Agri-Food.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"82 11","pages":"10648-10657"},"PeriodicalIF":4.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ps.70320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target-site mutations and phenotypic failure in Myzus persicae against pyrethroids and carbamates in central Europe","authors":"Jitka Stara,&nbsp;Jan Hubert,&nbsp;Pavla Pabiskova,&nbsp;Frantisek Kocourek","doi":"10.1002/ps.71181","DOIUrl":"10.1002/ps.71181","url":null,"abstract":"<p>BACKGROUND</p><p>The green peach aphid, <i>Myzus persicae</i>, is a major pest and virus vector in central European agriculture. Following the neonicotinoid seed treatment ban and under climate warming, its populations have increased, raising concerns about insecticide resistance.</p><p>RESULTS</p><p>This study assessed the phenotypic resistance and underlying target-site mutations in Czech populations of <i>M. persicae</i> from oilseed rape between 2022 and 2024. Bioassays with cypermethrin, pirimicarb, and acetamiprid revealed extreme and increasing pyrethroid resistance, whereas susceptibility to pirimicarb and acetamiprid remained high or improved compared to earlier periods. Molecular screening of 140 field-collected aphids identified four sodium channel mutations (L1014F, M918L, M918T, L932F) and the ace2 mutation S431F. Survival under insecticide selection strongly highlighted the functional role of specific mutations: the M918L mutation was the most prevalent among cypermethrin survivors (68.75%), confirming it as the primary mechanism of pyrethroid target-site resistance in these populations. Conversely, the S431F mutation reached complete fixation (100%) in pirimicarb survivors, serving as a definitive marker for carbamate resistance and indicating a high risk of rapid resistance development upon intensive use. Significant negative correlations between M918L and L1014F/L932F suggest divergent resistance lineages.</p><p>CONCLUSION</p><p>No clear geographical patterns were observed, indicating that resistance is driven by agricultural practices rather than regional factors. These findings highlight the rapid evolution of pyrethroid resistance and underscore the need for integrated pest management, including molecular monitoring and careful insecticide rotation, to sustain control of <i>M. persicae</i> in Czechia. © 2026 The Author(s). <i>Pest Management Science</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"82 11","pages":"10590-10603"},"PeriodicalIF":4.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ps.71181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148667818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved impact from the use of beneficial natural enemy species in Australian canola systems: challenges and required tools","authors":"Sarina Macfadyen,&nbsp;Basman Al-Jalely,&nbsp;Mukti Chalise,&nbsp;Maryam Ehsangar,&nbsp;Zorica Duric,&nbsp;Joanne Holloway,&nbsp;Andrew Hulthen,&nbsp;Precious Mavende,&nbsp;Paul Melloy,&nbsp;Svetlana Micic,&nbsp;Hazel Parry,&nbsp;Shovon Chandra Sarkar,&nbsp;Jessa Thurman,&nbsp;Maarten Van Helden,&nbsp;Xiaobei Wang,&nbsp;Wei Xu","doi":"10.1002/ps.71291","DOIUrl":"10.1002/ps.71291","url":null,"abstract":"<p>The concept of Integrated Pest Management (IPM) has developed into a practical framework for sustainable pest management and is widely adopted in modern farming systems. Beneficial arthropods (natural enemies, such as predators and parasitoids) have shown potential for use in pest management, leading to observable production benefits. However, using beneficials is perhaps the most challenging IPM component to adopt at scale and in Australian canola systems, adoption remains low. Here we consider the challenges for measuring impact from beneficials in a way that links to production benefits for growers. To get an industry perspective, we surveyed canola growers and their advisors to understand their constraints and priorities for using beneficials for improved pest management. There were few practices to support and conserve beneficials adopted at scale (use of selective insecticides was the most used practice), and significant knowledge gaps about what impacts growers were likely to see if certain practices were to be adopted. Despite these knowledge gaps, canola growers and their advisors aspired towards more active management of beneficials. From our synthesis, we outline the new tools that are needed, designed specifically for beneficials, and present a pathway forward to enable the greater use of beneficials. We suggest that incremental steps are required for proactive transitions rather than whole-system shifts driven by industry crises. © 2026 Commonwealth of Australia. <i>Pest Management Science</i> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"82 11","pages":"10540-10550"},"PeriodicalIF":4.2,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ps.71291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}