Sublethal tebuconazole exposure enhances pathogenicity and resistance development in Sclerotium rolfsii.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2025-09-22 DOI:10.1002/ps.70250

Ruiping Huang,Lin Zhou,Xuewei Mao,Jialin Duan,Yue Wang,Hong Li,Qinqin Wang,Li Wang,Daojie Wang

{"title":"Sublethal tebuconazole exposure enhances pathogenicity and resistance development in Sclerotium rolfsii.","authors":"Ruiping Huang,Lin Zhou,Xuewei Mao,Jialin Duan,Yue Wang,Hong Li,Qinqin Wang,Li Wang,Daojie Wang","doi":"10.1002/ps.70250","DOIUrl":null,"url":null,"abstract":"BACKGROUND\r\nTebuconazole, an exemplary triazole fungicide, was registered in China for controlling peanut southern blight (caused by Sclerotium rolfsii Sacc.) in 2025; however, mechanistic studies on its toxicological effects remain limited. This study evaluates tebuconazole's (EC10: 0.0029, EC25: 0.0099 and EC30: 0.0134 mg L-1) effects on S. rolfsii growth, virulence, antioxidant enzymes, and target gene expression to guide its scientific application.\r\n\r\nRESULTS\r\nCompared to the control, the sclerotia germination rate of S. rolfsii at 24 h was increased by 64.29, 89.34, and 71.43% under EC₁₀, EC₂₅, and EC₃₀ tebuconazole exposure treatments, respectively. The cell membrane permeability of S. rolfsii was reduced after 36 h of tebuconazole exposure; however, prolonged treatment disrupted membrane integrity and subsequently increased permeability. Sublethal tebuconazole exposure increases the virulence of S. rolfsii towards peanuts and promotes oxalic acid secretion. Antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) and expression of the target gene (SrCYP51) exhibit responsive activation under sublethal tebuconazole stress.\r\n\r\nCONCLUSION\r\nTebuconazole (EC₁₀, EC₂₅, and EC₃₀) may induce sclerotial germination, enhance virulence, and upregulate expression of a target gene in S. rolfsii, which could pose threats to both effective disease control and fungicide resistance development in this pathogen. The results advance the understanding of tebuconazole toxicity against S. rolfsii and guide its control strategies. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"1 1","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/ps.70250","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

BACKGROUND Tebuconazole, an exemplary triazole fungicide, was registered in China for controlling peanut southern blight (caused by Sclerotium rolfsii Sacc.) in 2025; however, mechanistic studies on its toxicological effects remain limited. This study evaluates tebuconazole's (EC10: 0.0029, EC25: 0.0099 and EC30: 0.0134 mg L-1) effects on S. rolfsii growth, virulence, antioxidant enzymes, and target gene expression to guide its scientific application. RESULTS Compared to the control, the sclerotia germination rate of S. rolfsii at 24 h was increased by 64.29, 89.34, and 71.43% under EC₁₀, EC₂₅, and EC₃₀ tebuconazole exposure treatments, respectively. The cell membrane permeability of S. rolfsii was reduced after 36 h of tebuconazole exposure; however, prolonged treatment disrupted membrane integrity and subsequently increased permeability. Sublethal tebuconazole exposure increases the virulence of S. rolfsii towards peanuts and promotes oxalic acid secretion. Antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) and expression of the target gene (SrCYP51) exhibit responsive activation under sublethal tebuconazole stress. CONCLUSION Tebuconazole (EC₁₀, EC₂₅, and EC₃₀) may induce sclerotial germination, enhance virulence, and upregulate expression of a target gene in S. rolfsii, which could pose threats to both effective disease control and fungicide resistance development in this pathogen. The results advance the understanding of tebuconazole toxicity against S. rolfsii and guide its control strategies. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

亚致死戊康唑暴露增强罗尔夫菌核菌的致病性和抗性发展。

背景：戊康唑是一种典型的三唑类杀菌剂，于2025年在中国注册，用于防治花生南枯萎病（由菌核菌引起）；然而，对其毒理学效应的机理研究仍然有限。本研究评价戊康唑（EC10: 0.0029、EC25: 0.0099、EC30: 0.0134 mg L-1）对罗氏弧菌生长、毒力、抗氧化酶及靶基因表达的影响，指导其科学应用。结果与对照相比，EC₁₀、EC₂₅和EC₃₀戊康唑暴露处理下，24 h的菌核发芽率分别提高了64.29、89.34和71.43%。替布康唑作用36h后，罗氏弧菌细胞膜通透性降低；然而，长时间的处理破坏了膜的完整性，随后增加了通透性。亚致死的戊康唑暴露会增加罗尔夫氏梭菌对花生的毒力，并促进草酸分泌。抗氧化酶活性（超氧化物歧化酶、过氧化物酶和过氧化氢酶）和靶基因（SrCYP51）的表达在亚致死戊康唑胁迫下表现出响应性激活。结论苯康唑（EC₁₀、EC₂₅和EC₃₀）可能会诱导S. rolfsii的菌核萌发，增强毒力，并上调靶基因的表达，这可能对该病原体的有效控制和杀菌剂抗性的发展构成威胁。研究结果有助于进一步了解戊康唑对罗氏弧菌的毒性作用，指导其防治策略。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.