Pest Management Science最新文献

{"title":"Developments of nanoparticle-mediated dsRNA delivery systems to control ToCV and TYLCV infections in plants.","authors":"Gentu Wu, Xiaolong Yang, Haolan Zhang, Haoran Hu, Huiyuan Zhang, Rui Wu, Mingjun Li, Hanqiu Chen, Ling Qing","doi":"10.1002/ps.70607","DOIUrl":"10.1002/ps.70607","url":null,"abstract":"<p><strong>Background: </strong>Tomato chlorosis virus (ToCV) and tomato yellow leaf curl virus (TYLCV) are two important plant viruses. Co-infection of them leads to exacerbated disease symptoms and poses a significant challenge for control. Using nanomaterials to package and deliver double-stranded RNA (dsRNA) for inducing RNA interference (RNAi) has emerged as a promising antiviral strategy in agriculture.</p><p><strong>Results: </strong>In this study, two established nanomaterials, chitosan quaternary ammonium salt (CQAS) and aminated mesoporous silica nanoparticles (MSN@NH₂), were selected to package dsRNAs targeting the TYLCV C1 gene, the ToCV RNA-dependent RNA polymerase (RdRP) gene, or the ToCV 1a polyprotein gene, forming CQAS/dsRNA and MSN@NH₂/dsRNA nanoparticles, respectively. The results indicated that these nanoparticles enhance the stabilities of dsRNAs. The efficacy of these nanoparticles against ToCV and TYLCV infection was assessed in tobacco cv. K326, Nicotiana benthamiana, and tomato cv. Micro-Tom plants. The results showed that these nanoparticles could significantly reduce viral gene transcription levels, with inhibition rates ranging from 33.5% to 99.5% depending on the nanoparticle formulation, target virus, and host plant. For instance, in N. benthamiana under co-infection, MSN@NH2/dsRNA nanoparticles inhibited TYLCV and ToCV by 99.5% and 79.8%, respectively. These findings demonstrate that both CQAS/dsRNA and MSN@NH2/dsRNA nanoparticles are effective in controlling ToCV and TYLCV single or co-infection in plants.</p><p><strong>Conclusion: </strong>Both CQAS and MSN@NH₂ effectively package and protect dsRNA from degradation. Our findings further support the notion that the nanoparticle-mediated RNAi is a promising strategy for virus disease management. This approach contributes to global efforts toward sustainable agriculture. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":"4955-4966"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato spotted wilt virus exploits Halloween genes to accelerate development of Frankliniella occidentalis.","authors":"Xiaobin Zheng, Junxia Shi, Zezhong Yang, Min Tao, Yanran Wan, Shaonan Liu, Kanghua Qian, Ying Zhang, Yunfan Zhang, Jiangjiang Yuan, Yingxi Tang, Hongyi Cao, Baoyun Xu, Youjun Zhang, Qingjun Wu","doi":"10.1002/ps.70600","DOIUrl":"10.1002/ps.70600","url":null,"abstract":"<p><strong>Background: </strong>Viruses and their insect vectors have evolved intricate co-evolutionary mechanisms to enhance transmission efficiency. This study aimed to investigate the impact of tomato spotted wilt virus (TSWV) infection on the development of Frankliniella occidentalis, with particular emphasis on the potential involvement of Halloween genes.</p><p><strong>Results: </strong>Genomic analysis identified seven Halloween genes. Transcriptome data from revealed significant upregulation of FoCYP307A1 and FoCYP306A1 during the nymphal and pupal stages in TSWV-infected individuals. These findings were validated by quantitative real-time polymerase chain reaction, which demonstrated a 1.5- to 3-fold increase in transcript levels of these genes during the nymphal, propupal, and pupal developmental stages. TSWV infection significantly shortened the nymphal and pupal developmental durations by 1 day and 0.6 days, respectively. RNA interference (RNAi)-mediated silencing of FoCYP307A1 and FoCYP306A1 significantly prolonged the nymphal and pupal development time, accompanied by a 27.5% and 55% reduction in 20-hydroxyecdysone (20E) levels, respectively. In addition, the transcript levels of 20E key signaling pathway genes, including FoUSP, FoBr-C, FoDHR3, FoE74A, and FoE75, decreased by 16-67%. Importantly, supplementation with 20E effectively reversed the developmental delays caused by RNAi-induced silencing of FoCYP307A1 and FoCYP306A1. Collectively, these results suggest that FoCYP307A1 and FoCYP306A1 play a critical role in mediating TSWV-facilitated development in thrips.</p><p><strong>Conclusion: </strong>These findings provide valuable insights into how TSWV exploits ecdysone biosynthesis to accelerate development in insect vectors, offering important implications for integrated pest and disease management strategies. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":"4931-4940"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of UAVs in precision pesticide spraying and environmental safety by combating pesticide resistance and safety of natural enemies.","authors":"Muhammad Zeeshan, Haoran Li, Ying Li, Guoqiang Zhang, Yali Zhang, Xiaoqiang Han","doi":"10.1002/ps.70872","DOIUrl":"https://doi.org/10.1002/ps.70872","url":null,"abstract":"<p><p>The use of unmanned aerial vehicles (UAVs) has emerged as a promising tool to maximize agricultural productivity and is useful in precision and sustainable insect pest control, with less impact on the environment and human health. In this review, we revealed how UAVs are valuable in dealing with pesticide resistance issues, reducing the ecological footprint of practices during pest management, and how they are safer for natural enemies. These UAVs have the ability to considerably reduce the overall dosage of insecticides with targeted delivery, which minimizes the risk of resistance development in insect pests against tested pesticides. Moreover, the addition of advanced technologies, such as computer vision systems, allows UAVs to optimize pesticide usage based on real-time data. The delivery of biological control agents by UAVs further supports ecological sustainability. However, a careful consideration is needed on the impact of UAV-applied chemicals on non-target organisms and ecosystems. To minimize drift, strategies such as optimized flight parameters, use of suitable nozzles, and integration of adjuvants should be adopted, which are important to ensure environmental safety. Future research on UAV technology, application methods, pesticide formulation, and natural enemy delivery is crucial to maximize the benefits of this innovative technique and ensure the safety of agroecosystems. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catalases act as putative oral secretion effectors in Tuta absoluta, suppressing tomato oxidative defenses and providing safe RNAi targets for pest control.","authors":"Li Yang, Deqian Liu, Shizhi Liu, Zhuting Zhang, Kangkang Xu, Guy Smagghe, Wenjia Yang","doi":"10.1002/ps.70884","DOIUrl":"https://doi.org/10.1002/ps.70884","url":null,"abstract":"<p><strong>Background: </strong>Tuta absoluta is a globally destructive invasive Solanaceae pest with widespread resistance to conventional insecticides, highlighting the urgent need for eco-friendly and sustainable control strategies. Catalase (CAT) is a key antioxidant enzyme that primarily scavenges intracellular hydrogen peroxide (H₂O₂) to maintain redox homeostasis. CATs have also been detected in the oral secretions (OSs) of some herbivorous insects, suggesting an additional extracellular role in manipulating plant defense during feeding making CATs innovative targets for pest management. Here, we systematically identified CATs in Tuta absoluta and evaluated their functions and suitability as RNA interference (RNAi) targets.</p><p><strong>Results: </strong>Nine CATs were identified, with TaCAT1-TaCAT3 clustered phylogenetically with known insect OS-associated CATs. These genes showed high expression in larval heads and guts, induced by tomato feeding, indicating roles at the insect-plant interface. Silencing these CATs significantly impaired larval performance: individual double-stranded RNAs (dsRNAs) caused 69% mortality, while combined targeting (dsCAT-mix) achieved 80% mortality, accompanied by reduced pupation and smaller pupae. Functionally, CAT silencing induced tomato leaves H₂O₂ accumulation, and activated jasmonic acid pathway while suppressing salicylic acid signaling. These results demonstrate that CATs facilitate larval feeding by suppressing plant oxidative and hormonal defenses. Importantly, dsCAT treatments had no negative effects on predator Nesidiocoris tenuis, underscoring biosafety and specificity.</p><p><strong>Conclusion: </strong>These findings uncover a previously underappreciated role of CATs as putative OS effectors in a lepidopteran pest, validating them as effective, selective, and eco-friendly RNAi targets. This work provides mechanistic insight into insect-plant interactions and a practical foundation for sustainable RNAi-based strategies against Tuta absoluta. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem borer pest outbreaks in cereal crops are associated with summer drought under climate change: A national-scale study.","authors":"Junfeng Shui, Jie Liu, Lei Zhang, Zihua Zhao, Leyun Wang","doi":"10.1002/ps.70868","DOIUrl":"https://doi.org/10.1002/ps.70868","url":null,"abstract":"<p><strong>Background: </strong>Stem borer pests can cause substantial economic losses in growing Poaceae crops by damaging their stalks, where their concealed boring habitats shield them from natural enemies, human detection, and limit pesticide penetration. Studying the influence of climate factors on these pests provides critical information for sustainable pest control and outbreak prediction under global changes. This study systematically investigated the field outbreak of important stem borers in corn, rice, and wheat: Ostrinia furnacalis Guenée (Lepidoptera: Crambidae), Chilo suppressalis Walker (Lepidoptera: Crambidae), Scirpophaga incertulas Walker (Lepidoptera: Pyralidae), and Meromyza saltatrix Linnaeus (Diptera: Chloropidae) in China from 1987 to 2019 in relations to climate change.</p><p><strong>Results: </strong>Across the studied species, we found that monthly precipitation has regional and species-specific effects on the outbreaks of borers. Furthermore, we conducted meta-regressions of coefficients of models to summarize general effects of seasonal climate changes on borer pest outbreaks in 3 grain crops and sugarcane across all study regions. We found a universal phenomenon across study species in subtropical areas that annual outbreaks of borers were more severe with drier summers.</p><p><strong>Conclusion: </strong>This study not only provides critical knowledge for understanding stem borer responses to climate change in Poaceae crops but also establishes a forecasting framework for pest outbreaks. Our findings establish a scientific outbreak alarm for major pest outbreaks in main cereal crops and sugar crops. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TSHFS: a two-stage hyperspectral feature selection approach for congeneric rice planthopper species classification.","authors":"Dongmei Chen, Guiji Sun, Bing Li, Jv Luo, Shuhua Liu, Xianfeng Zhou, Jingcheng Zhang","doi":"10.1002/ps.70616","DOIUrl":"10.1002/ps.70616","url":null,"abstract":"<p><strong>Background: </strong>Rice planthoppers, particularly the congeneric species Nilaparvata lugens (Stål), Nilaparvata bakeri (Muir), and Nilaparvata muiri (China), are significant pests in rice production. Their morphological similarity poses a considerable challenge for conventional classification methods. To address this issue, we propose a two-stage hyperspectral feature selection (TSHFS) method. The TSHFS framework first employs competitive adaptive reweighted sampling (CARS) to identify informative spectral bands, followed by the adaptive gradient boosting decision tree for feature refinement and importance analysis. Additionally, first-order derivative spectral preprocessing is applied to enhance spectral contrast and improve feature discrimination.</p><p><strong>Results: </strong>Extensive experiments on hyperspectral data from the three congeneric species demonstrate that TSHFS significantly enhances classification performance. Specifically, TSHFS achieves the highest accuracy of 86.42% with an F1 score of 86.41%, outperforming models trained on raw spectral data (accuracy 64.17%, F1 score 64.27%) and CARS-selected features alone (accuracy 66.33%, F1 score 66.52%). Furthermore, integrating first-order spectral preprocessing with CARS-selected bands maintains a high accuracy of 85.63% while achieving effective feature selection.</p><p><strong>Conclusion: </strong>TSHFS effectively selects relevant spectral features, removes redundancy, optimizes spectral data utilization, and reduces computational complexity while improving classification accuracy. The results are significant as they provide a reliable method for distinguishing morphologically similar rice planthopper species, which advances automated pest identification in agricultural applications and can improve pest management strategies in rice production. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":"5040-5053"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146148597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species identification and pathogenicity study of Alternaria fungi causing pear black spot disease in Anhui Province.","authors":"Dandan Meng, Mingzhi Liu, Xue Yang, Xu Dong, Mingna Sun, Yue Chu, Zhou Tong, Xiaotong Yi, Jiayun Fu, Tongchun Gao, Jinsheng Duan","doi":"10.1002/ps.70611","DOIUrl":"10.1002/ps.70611","url":null,"abstract":"<p><strong>Background: </strong>Black spot disease is a significant disease during the growth period of pear trees. The species and pathogenicity of Alternaria fungi causing black spot disease in Anhui Province are still unclear.</p><p><strong>Results: </strong>In this study, through phylogenetic analysis of multi-gene tandem sequences, pathogens causing pear black spot disease (PBS) in Anhui Province were identified, primarily Alternaria alternata, Alternaria gaisen and Alternaria tenuissima, with A. alternata being the dominant species (41.18%). The single nucleotide polymorphism (SNP) density of Alt a1 was much higher than that of other genes, and the codon bias was affected by both natural selection and mutation. The codon bias and the amino acid ratio of Alt a1 in A. alternata and A. gaisen were highly consistent, and A. tenuissima was quite different from them. The pathogenicity of A. alternata was significantly negatively correlated with the daily mycelia growth rate, dry weight of mycelia and alternariol production, and significantly positively correlated with tenuazonic acid (TeA) and tentoxin production. TeA might be the main virulence factor in the pathogenic process of A. alternata.</p><p><strong>Conclusion: </strong>This study presents novel ideas for classifying Alternaria fungi and provides a theoretical basis for establishing prevention and control technologies for PBS control and toxin reduction. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":"4990-5005"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trunk injection of fluopyram and chlorfenapyr for efficient and long-lasting control of pine wilt disease.","authors":"Wanjun Zhang, Xianxiu Hu, Guohong Sun, Tianjian Li, Lvxin Huang, Wenbai Tian, Hao Cheng, Tingting Chen, Fang Tang, Jianren Ye","doi":"10.1002/ps.70517","DOIUrl":"10.1002/ps.70517","url":null,"abstract":"<p><strong>Background: </strong>Chemical control is an important strategy for managing pine wilt disease (PWD). However, traditional trunk injection agents (TIAs) are constrained by limited variety and insufficient persistence. In addition, although some novel nematicides show strong activity, their large-scale forest use is restricted by high costs. Therefore, the development of cost-effective, highly efficient, and synergistic nematicidal formulations is urgently needed to advance sustainable PWD management.</p><p><strong>Results: </strong>This study compared the performance of combination TIAs with single-agent TIAs and a traditional TIA in terms of nematicidal activity, xylem translocation, residue dynamics, efficacy duration, and tree safety in Pinus massoniana. All six TIA formulations exhibited nematicidal activity, with fluopyram_chlorfenapyr-37 (FLU_CHL-37) TIA markedly suppressing nematode reproduction in detached branch experiments. Field trials demonstrated that all TIAs spread throughout P. massoniana within 3 months; by 18 months, residual concentrations of CHL-46, CHL-49, FLU_CHL-35, and FLU_CHL-37 were significantly higher than those of FLU-11, FLU-19, and a 20% emamectin benzoate soluble liquid (EB SL) positive control in branches 3 m above the injection site and in apical branches. Notably, FLU_CHL-37 achieved 100% control efficacy at 360 days post-inoculation, outperforming the other treatments. Safety assessments showed only slight, short-term changes in chlorophyll, malondialdehyde, and soluble protein content, all normalizing within 21 days, while local damage around the injection site was significantly less pronounced than that caused by the 20% EB SL control.</p><p><strong>Conclusion: </strong>These results demonstrate that FLU_CHL-37 combines high activity, prolonged efficacy, strong translocation, and robust safety, providing theoretical and practical support for the development of novel PWD control agents. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":"4114-4123"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analysis of corm yield responses to chemical and biological approaches for managing taro leaf blight disease.","authors":"Michael O Adu, Emmanuel Afutu, Paul Agu Asare, Kwadwo K Amoah, Mishael Amoah Nyarko, Kwabena A Sanleri","doi":"10.1002/ps.70878","DOIUrl":"https://doi.org/10.1002/ps.70878","url":null,"abstract":"<p><strong>Background: </strong>Taro leaf blight (TLB), caused by Phytophthora colocasiae, inflicts yield losses of up to 90% and annual economic damage exceeding USD 1.4 billion in West Africa. Despite a growing body of literature on chemical and biological disease management, no quantitative synthesis has compared their efficacy for protecting taro corm yield. This meta-analysis quantified the effects of chemical fungicides and biological control treatments on taro yield.</p><p><strong>Results: </strong>Literature searches of Scopus and Google Scholar (December 2024) identified 21 eligible studies published between 1980 and 2022, yielding 168 treatment comparisons (91 chemical, 77 biological) across five countries. A multilevel random-effects meta-analysis with cluster-robust variance estimation (RVE) was applied to account for correlated effect sizes within studies. Chemical treatments produced a large, statistically significant pooled effect (Hedges' g = 1.072, 95% CI: 0.650-1.494, P < 0.001; k = 74 effect sizes from 15 studies). This result was robust across all sensitivity analyses, including outlier removal (g = 0.983), complete-case standard deviations (g = 0.968), and bias adjustment via trim-and-fill (g = 0.653). Multi-component fungicide formulations outperformed single-active-ingredient products, and effect sizes increased modestly over four decades. Biological treatments, based on only six unique studies contributing 73 effect sizes, yielded a non-significant pooled effect under RVE (g = 0.508, 95% CI: -0.497 to 1.514, P = 0.249). Extreme heterogeneity was observed (I² = 93.7%), and trim-and-fill adjustment further reduced the estimate to near zero (g = 0.011). Median biological treatment yields were 7.9% below untreated control yields.</p><p><strong>Conclusion: </strong>Chemical fungicides provide robust, reproducible yield protection against TLB across geographic regions and experimental conditions. The evidence base for biological control of TLB is currently insufficient to support conclusions about efficacy: the available studies are few, the data are heterogeneous, imputation rates for standard deviations are high, and accounting for study-level clustering eliminates the apparent statistical significance of the pooled estimate. Standardized reporting protocols and targeted trials in major taro-producing regions are needed before biological or integrated management strategies can be recommended on an evidence-based basis. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target-gene overexpression and metabolic enhancement confer bentazone resistance in Amaranthus retroflexus.","authors":"Chang Liu, Shen Wang, Di Ma, Xuelian Liu, Xiaofeng Xu, Wenpeng Sun, Chunjie Li, Zhonghua Sun, Chunhong Teng","doi":"10.1002/ps.70865","DOIUrl":"https://doi.org/10.1002/ps.70865","url":null,"abstract":"<p><strong>Background: </strong>Resistance to photosystem II (PSII)-inhibiting herbicides is increasingly widespread in Amaranthus retroflexus, a major weed in soybean production, yet the underlying mechanisms remain unclear. This study aimed to characterize the molecular basis of bentazone (BTZ) resistance in this species.</p><p><strong>Results: </strong>BTZ-resistant genotype (B24) showed a resistance index of 11.30 relative to susceptible B06. No target-site mutations were found, but psbA expression was 1.14-to 2.42-fold higher in B24. Transcriptomics and RT-qPCR identified 11 significantly upregulated genes, including four glycosyltransferases, three ABC transporters, and four cytochrome P450s. Molecular docking revealed strong binding of BTZ with ArUGT86A2, ArABCG5, and ArCYP71A1, and heterologous expression in yeast confirmed that ArCYP71A1 confers BTZ resistance.</p><p><strong>Conclusion: </strong>This research provides the first evidence that both target-site overexpression and metabolic enhancement drive BTZ resistance in A. retroflexus. These findings establish a foundation for mechanistic studies and improved management of resistant populations. © 2026 Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}