Pest Management Science最新文献

{"title":"Whole-genome sequencing reveals novel ryanodine receptor mutations P4568L and Y4701D conferring high-level chlorantraniliprole resistance in Spodoptera litura (Lepidoptera: Noctuidae).","authors":"Yizhou Chen,Lisa Bird,Thi Ht Nguyen,Grant A Herron,Duong T Nguyen","doi":"10.1002/ps.70238","DOIUrl":"https://doi.org/10.1002/ps.70238","url":null,"abstract":"BACKGROUNDSpodoptera litura is a significant insect pest that attacks many agricultural crops, including horticultural, cotton, and legume crops. It has developed resistance to various insecticides, including chlorantraniliprole, posing significant challenges for pest management. Recently, a field-collected population of S. litura (BM28x) from northwestern Australia was found to be more than 1000-fold less susceptible to chlorantraniliprole than a laboratory susceptible strain. Therefore, understanding the mechanism of this insecticide resistance and developing DNA-based molecular diagnostic assays are critical for developing and implementing sustainable pest control strategies for S. litura.RESULTSWe mapped the chlorantraniliprole resistance gene to chromosome 26 using whole-genome sequencing and linkage analysis. A combination of the novel mutations P4568L and Y4701D in the ryanodine receptor (RyR) gene was shown to cause high-level resistance to chlorantraniliprole in the BM28x strain. A molecular diagnostic assay was developed to monitor these resistance mutations in field populations. Mutations P4568L and Y4701D were widely distributed in field populations across Western Australia and the Northern Territory.CONCLUSIONThis research elucidates the genetic mechanisms underlying chlorantraniliprole resistance in S. litura and provides the first evidence of the Y4701D mutation conferring diamide resistance in Noctuidae. These findings enable the development of rapid molecular diagnostic assays which are vital for proactive resistance management. Given that the resistance is recessive in nature it can remain undetected by simple conventional bioassays until it becomes widespread. The findings significantly advance the field of resistance management by facilitating timely interventions to prevent resistance development, thereby supporting more effective integrated pest management. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"23 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive lethal effects and transgenerational impact of Metarhizium anisopliae on Anopheles sinensis across all life stages","authors":"Xiaoyu Liu, Yujie Qin, Ou Xu, Wen Luo, Zhengbo He, Hengqing Ji, Guoxiong Peng, Yuxian Xia, Yueqing Cao","doi":"10.1002/ps.70224","DOIUrl":"https://doi.org/10.1002/ps.70224","url":null,"abstract":"Mosquitoes are major vectors of disease and pose a significant threat to public health. Chemical insecticides face resistance and environmental issues, prompting the need for eco-friendly alternatives such as entomopathogenic fungi. However, their potential across mosquito life stages remains underexplored. As a primary malaria vector in East Asia, <i>Anopheles sinensis</i> remains understudied in terms of biological control approaches.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"7 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic bacterial engineering: a novel approach for pine wilt disease management in Pinus massoniana.","authors":"Da Li,JianRen Ye,YuWei Tang,YueYuan Peng,LiNa He,MengLu Yu,LvXin Huang,XiaoQin Wu","doi":"10.1002/ps.70220","DOIUrl":"https://doi.org/10.1002/ps.70220","url":null,"abstract":"BACKGROUNDPine wilt disease (PWD) is a globally devastating forest disease caused by the pine wood nematode (Bursaphelenchus xylophilus). Chemical control plays a role in the prevention and control of PWD, but it also raises concerns regarding environmental safety and drug resistance. Seeking novel strategies and technologies to manage PWD has long been a matter of concern. The aim of this study was to increase the resistance of pine trees to PWD through the application of the transgenic engineered bacterium Pseudomonas koreensis BM06-P60.RESULTSA dominant endophytic bacterium, P. koreensis BM06, was isolated from healthy stems of Pinus massoniana. This strain was capable of efficiently and stably colonizing P. massoniana and significantly promoted the growth of its seedlings. The purified peptidase P60 protein was able to disintegrate the nematode cuticle, damage the intestinal tissues, and exert adverse effects on the nematodes. The engineered strain BM06-P60, which expresses peptidase P60, exhibited significantly enhanced nematicidal activity. Greenhouse disease control tests demonstrated that the engineered strain BM06-P60 exhibited 70% efficacy against PWD. Preliminary ecological safety evaluations indicated that BM06-P60 is a promising and safe candidate for biocontrol engineering.CONCLUSIONThe application of the engineered strain BM06-P60 effectively controls PWD. This study provides a novel microbial resource for the prevention and control of PWD. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"4 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the mechanisms for plant-derived linalool control of anthracnose in sorghum.","authors":"Wangdan Xiong,Xinfeng Jia,Tianyu Wang,Guo Wei,Yanjun Guo","doi":"10.1002/ps.70240","DOIUrl":"https://doi.org/10.1002/ps.70240","url":null,"abstract":"BACKGROUNDSorghum anthracnose, caused by Colletotrichum sublineola, poses a major threat to sorghum production, causing substantial damage globally. Linalool, a terpenoid synthesized by plants, plays a crucial role in defense against pathogens. This study explored the dual defense roles of the plant-synthesized terpenoid linalool through both host resistance potentiation and direct antifungal action.RESULTSFollowing fungal infection, linalool levels increased significantly in sorghum leaves, with resistant genotypes exhibiting higher linalool accumulation than susceptible ones. A strong positive correlation between linalool content and disease resistance suggested its importance in fungal defense. Transcriptomic profiling identified Sobic.004G019400 as the key terpene synthase (TPS) gene responsive to fungal infection. This was validated functionally through heterologous expression and gas chromatography-mass spectrometry (GC-MS) analysis. Physiological assessments demonstrated that linalool application reduced lesion areas and alleviated declines in photosynthetic activity and transpiration in infected plants. The study also indicated that linalool inhibited C. sublineola spore germination and hyphal growth. Mechanistic investigations demonstrated that linalool effectively inhibited hyphal growth by inducing malondialdehyde overaccumulation, which caused significant damage to the fungal cell membrane and structure. This damage was evidenced by increased electrical conductivity and nucleic acid leakage. Transcriptomic analyses further revealed that linalool disrupted pathways in C. sublineola, including oxidative phosphorylation and the tricarboxylic acid (TCA) cycle.CONCLUSIONThese findings not only identify Sobic.004G019400 as a molecular target for breeding anthracnose-resistant sorghum varieties, but also establish linalool as a functional antimicrobial agent with potential applications for integrated pest management strategies. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"4 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of host age, parasitoid age, and superparasitism on reproductive fitness of Macrocentrus cingulum (Hymenoptera: Braconidae), a larval polyembryonic parasitoid.","authors":"Qianqian Mi,Feng Zhang,Jian Hu","doi":"10.1002/ps.70212","DOIUrl":"https://doi.org/10.1002/ps.70212","url":null,"abstract":"BACKGROUNDMacrocentrus cingulum Brischke is a polyembryonic endoparasitoid of Ostrina furnacalis Guenée larvae. The interactions between parasitoid age, host age, and superparasitism could affect reproductive attributes such as parasitism rate and brood size, changing the efficiency of mass-rearing programs producing M. cingulum.RESULTSThe parasitism rate of M. cingulum on O. furnacalis larvae was significantly affected by host age, parasitoid age, and their interactions. Macrocentrus cingulum preferred parasitizing host larvae at late 3rd instars over early or late 4th instars. Older female parasitoids (5, 6, or 7 days after emergence) caused higher parasitism levels on three tested host ages than did younger parasitoids, likely because M. cingulum is synovigenic and the older females had a greater oocyte load in their oviducts. The age of O. furnacalis larvae significantly affected brood size but did not change the developmental time of M. cingulum. Late stage, third instars of O. furnacalis supported the largest parasitoid broods. Both brood size and body size of offspring were influenced significantly by superparasitism. Doubly-stung hosts produced most parasitoid offspring while the body size was smallest.CONCLUSIONIn summary, late 3rd instars of O. furnacalis larvae, combined with adult female parasitoids five or more days old and with two stings were optimal for mass rearing of M. cingumum in the laboratory. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"45 1 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the resistance risk and mechanism of mefentrifluconazole in Colletotrichum scovillei.","authors":"Niuniu Shi,Qinghai Wang,Jingyi He,Xuefang Zheng,Yixin Du,Fangluan Gao","doi":"10.1002/ps.70246","DOIUrl":"https://doi.org/10.1002/ps.70246","url":null,"abstract":"BACKGROUNDColletotrichum scovillei is a major fungal pathogen responsible for anthracnose disease, posing a growing threat to global chili production. Mefentrifluconazole, a novel isopropanol-triazole fungicide, exhibits strong efficacy against a broad spectrum of plant pathogens. However, the resistance risk of mefentrifluconazole in Colletotrichum scovillei and the associated molecular mechanisms remain poorly characterized.RESULTSIn this study, we evaluated the sensitivity of 102 Colletotrichum scovillei isolates to mefentrifluconazole, revealing a half-maximal effective concentration (EC50) value of 0.6924 ± 0.1482 μg mL-1. Through fungicide adaptation, seven stable resistant mutants were generated from four sensitive parental isolates, with resistance factors ranging from 3.73 to 26.51. Compared to their parental isolates, the resistant mutants displayed similar or reduced fitness in terms of growth, sporulation and pathogenicity. Cross-resistance assays indicated that mefentrifluconazole exhibited positive cross-resistance with difenoconazole, propiconazole and prochloraz, but not with pyraclostrobin, florylpicoxamid or fluazinam. Further biochemical analysis demonstrated that mefentrifluconazole treatment resulted in a significantly higher inhibition rate of ergosterol biosynthesis in parental isolates relative to resistant mutants. A similar finding was observed in cell membrane damage assessment. Molecular investigations revealed no mutations in CYP51 paralogs among resistant mutants; however, quantitative analysis confirmed the overexpression of CYP51 paralogs in these isolates following mefentrifluconazole exposure.CONCLUSIONTogether, there is a low risk of Colletotrichum scovillei developing resistance to mefentrifluconazole, and the induced overexpression of CYP51 paralogs may contribute to potential mefentrifluconazole resistance in this pathogen. These findings offer significant implications for formulating effective management strategies against anthracnose. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"127 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145077907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of two genes related to the stresses of low temperature and modified atmosphere in Zeugodacus tau.","authors":"Qing Sun,Guangxiu Cui,Guoping Zhan,Zhihong Li,Shaokun Guo","doi":"10.1002/ps.70256","DOIUrl":"https://doi.org/10.1002/ps.70256","url":null,"abstract":"BACKGROUNDZeugodacus tau (Walker), a globally significant quarantine pest, jeopardizes fruit and vegetable production through direct crop damage and trade restrictions. The implementation of effective quarantine management measures constitutes a critical component in mitigating biosecurity risks associated with pest proliferation. Integrated low temperature and modified atmosphere treatment has emerged as an environmentally sustainable approach, demonstrating significant efficacy against Zeugodacus tau infestations while providing sustainable phytosanitary solutions for postharvest management.RESULTThis study examined the effects of low-temperature treatments (2 °C/5 °C/8 °C) and combined low temperature and modified atmosphere (2 °C, 5 °C, 8 °C, and 3% oxygen (O2)) on third-instar larvae, the most resilient developmental stage of Zeugodacus tau. The 8 °C + 3% O2 treatment induced significantly higher mortality (10-day exposure) than temperature-only conditions, highlighting its potential for postharvest pest management. Transcriptomic analysis revealed two key tolerance-associated genes, aminopeptidases EY (EY) and UTP-glucuronosyltransferase (UGT), with RNA interference of key genes amplifying mortality by 60-80%, confirming their critical role in survival under dual stress.CONCLUSIONThis study data systematically demonstrated that third-instar larvae were the most tolerant life stage under all treatments and then identified two key tolerance genes associated to stress adaptation. These dual findings significantly advance integrated pest management (IPM) strategies by integrating physicochemical approaches with molecular insights, offering actionable strategies to mitigate Zeugodacus tau infestations in agricultural supply chains, and supporting the safe global trade of fresh produce and national biosecurity frameworks. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"37 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olfactory-mediated chemotaxis toward a key host plant leaf volatile in the fall webworm.","authors":"Biao Liu,Wenjing Zhang,Jianqiao Li,Jianghua Sun,Dingze Mang","doi":"10.1002/ps.70239","DOIUrl":"https://doi.org/10.1002/ps.70239","url":null,"abstract":"BACKGROUNDOlfaction is essential for the survival and reproductive success of insects, mediating critical behaviors such as host plant location, mating, and oviposition. The fall webworm, Hyphantria cunea, is a highly polyphagous and invasive pest that infests a wide range of deciduous trees and shrubs. Its ability to efficiently locate suitable host plants primarily guided by olfactory cues, particularly during the larval stage, is a major contributor to its ecological success. While previous studies have examined the general olfactory and gustatory systems of H. cunea, the specific volatile compounds that elicit larval chemotactic responses remain largely uncharacterized.RESULTBehavioral and electrophysiological assays identified that the host plant volatile cis-3-hexenyl acetate (Z3HAc) functions as a chemoattractant for H. cunea larvae. Quantitative real-time polymerase chain reaction (PCR) and calcium imaging revealed that the antennal receptor HcOr3 is highly expressed and selectively responsive to Z3HAc. RNA interference-mediated knockdown of HcOr3 significantly reduced larval attraction to Z3HAc. Furthermore, single sensillum recordings confirmed that HcOr3 knockdown markedly decreased antennal sensitivity to Z3HAc, establishing its critical role in detecting this volatile compound.CONCLUSIONWe identified Z3HAc as a key attractant in host plants for H. cunea larvae and demonstrated that the receptor HcOr3 likely mediates host plant recognition. This work enhances our understanding of the molecular basis of host-seeking behavior in this invasive pest and highlights HcOr3 as a promising target for species-specific pest control. Our findings elucidate the link between olfactory perception and chemotactic response to natural plant volatiles in folivorous insects. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"78 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance risk and mechanism of fludioxonil against Exserohilum turcicum, the causal agent of northern corn leaf blight.","authors":"Xiaoning Song,Xingyu Ren,Xiangli Li,Wenrong Xian,Yunxiang Liu,Zhiqing Ma,Juntao Feng,Xili Liu,Peng Lei,Yong Wang","doi":"10.1002/ps.70244","DOIUrl":"https://doi.org/10.1002/ps.70244","url":null,"abstract":"BACKGROUNDNorthern corn leaf blight (NCLB), caused by Exserohilum turcicum, is one of the most important fungal diseases in corn production worldwide. The current study explored the resistance risk and mechanism of fludioxonil against E. turcicum.RESULTSThe median effective concentration (EC50) values of fludioxonil against 100 isolates of E. turcicum was in the range 0.0032-0.1809 μg mL-1, with a mean of 0.0447 μg mL-1. Eight fludioxonil-resistant mutants of E. turcicum were acquired. Compared with their wild-type strains, the resistant mutants showed decreased biological fitness. A fungicide sensitivity test showed that fludioxonil resistance mutations of E. turcicum also reduced the sensitivity of mutant strains to fluazinam. Importantly, a new point mutation D905N in the EtOs1 gene was detected. Molecular docking further showed that this point mutation led to a decreased binding affinity between histidine kinases and ATP. Moreover, the activity of histidine kinases in the resistant mutants decreased remarkably. Field tests showed that fludioxonil at 20 g ha-1 exhibited >80% efficiency against NCLB.CONCLUSIONThese data indicated that the resistance risk of fludioxonil against E. turcicum is low. Fludioxonil is a promising alternative fungicide for controlling NCLB. © 2025 Society of Chemical Industry.","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"80 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an eco-friendly RNAi yeast attractive targeted sugar bait that silences the shaker gene in spotted-wing drosophila, Drosophila suzukii.","authors":"Keshava Mysore, Jackson Graham, Teresia M Njoroge, Akilah T M Stewart, Saisuhas Nelaturi, Molly Duman-Scheel","doi":"10.1002/ps.70228","DOIUrl":"https://doi.org/10.1002/ps.70228","url":null,"abstract":"<p><strong>Background: </strong>Drosophila suzukii, or spotted-wing drosophila (SWD), (Diptera: Drosophilidae), are invasive vinegar flies of East Asian origin that have wreaked havoc on the small fruit and berry industry. In locations where SWD are well established, weekly chemical insecticide applications are necessary, resulting in increased economic costs, unwanted environmental impacts ensuing from loss of non-targeted organisms, and the eventual emergence of populations that are resistant to these insecticides. It is therefore critical that new classes of biorational pesticides and cost-effective technologies for controlling SWD are identified.</p><p><strong>Results: </strong>Here, we used the attractive properties of Saccharomyces cerevisiae, baker's yeast, which was designed to express an RNA interference (RNAi) pesticide that specifically targets the SWD Shaker (Sh) gene, to lure and kill flies that feed on the yeast, which was delivered in a feeder as a component of an attractive targeted sugar bait (ATSB). The yeast, which was heat killed prior to preparation of the ATSB, silenced the Sh gene, resulting in severe neural defects and 96 ± 9% fly mortality in laboratory trials. The RNAi yeast was successfully fed to the flies in an easily assembled soda bottle feeder that continuously rewetted the yeast with soda, which lured and killed the flies in simulated field trials. Despite this toxicity observed in SWD, consumption of the yeast had no impact on the survival of other dipteran insects.</p><p><strong>Conclusion: </strong>This promising ATSB technology, which was prepared with a new class of RNAi yeast insecticides, could one day be an effective component in integrated SWD control programs. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}