Resistance risk and mechanism of fludioxonil against Exserohilum turcicum, the causal agent of northern corn leaf blight.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2025-09-18 DOI:10.1002/ps.70244

Xiaoning Song,Xingyu Ren,Xiangli Li,Wenrong Xian,Yunxiang Liu,Zhiqing Ma,Juntao Feng,Xili Liu,Peng Lei,Yong Wang

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Abstract

BACKGROUND Northern corn leaf blight (NCLB), caused by Exserohilum turcicum, is one of the most important fungal diseases in corn production worldwide. The current study explored the resistance risk and mechanism of fludioxonil against E. turcicum. RESULTS The median effective concentration (EC50) values of fludioxonil against 100 isolates of E. turcicum was in the range 0.0032-0.1809 μg mL-1, with a mean of 0.0447 μg mL-1. Eight fludioxonil-resistant mutants of E. turcicum were acquired. Compared with their wild-type strains, the resistant mutants showed decreased biological fitness. A fungicide sensitivity test showed that fludioxonil resistance mutations of E. turcicum also reduced the sensitivity of mutant strains to fluazinam. Importantly, a new point mutation D905N in the EtOs1 gene was detected. Molecular docking further showed that this point mutation led to a decreased binding affinity between histidine kinases and ATP. Moreover, the activity of histidine kinases in the resistant mutants decreased remarkably. Field tests showed that fludioxonil at 20 g ha-1 exhibited >80% efficiency against NCLB. CONCLUSION These data indicated that the resistance risk of fludioxonil against E. turcicum is low. Fludioxonil is a promising alternative fungicide for controlling NCLB. © 2025 Society of Chemical Industry.

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氟菌腈对北方玉米叶枯病病原菌turcicum的抗性风险及机理研究。

玉米叶枯病（NCLB）是玉米生产中最重要的真菌病害之一，是由灰枯菌（exserhilum turcicum）引起的。本研究探讨了嘧菌腈对黄芽孢杆菌的抗性风险及其作用机制。结果氟菌腈对100株黄芽孢杆菌的中位有效浓度（EC50）为0.0032 ~ 0.1809 μg mL-1，平均值为0.0447 μg mL-1。获得了8个抗氟虫腈突变体。与野生型相比，抗性突变体的生物适应度降低。一项杀菌剂敏感性试验表明，鸢尾芽孢杆菌对氟嘧唑尼的抗性突变也降低了突变菌株对氟嗪南的敏感性。重要的是，在EtOs1基因中检测到一个新的点突变D905N。分子对接进一步表明，这种点突变导致组氨酸激酶与ATP的结合亲和力降低。耐药突变体组氨酸激酶活性显著降低。田间试验表明，在20 g / ha-1浓度下，氟菌腈对NCLB的防治效果为80 ~ 80%。结论氟菌腈对大黄菌的耐药风险较低。氟菌腈是一种很有前途的防治NCLB的杀菌剂。©2025化学工业协会。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.