J. Joe Hull, Kevin P. Le, Inana X. Schutze, Chan C. Heu, Roni J. Gross, Peter G. Fabrick, Joseph A. Rodriguez, Aiden M. Hull, Daniel Langhorst, Jeffrey A. Fabrick, Colin S. Brent
{"title":"RNAi-Mediated Knockdown of Tektins Does Not Affect Male Fertility in Lygus hesperus","authors":"J. Joe Hull, Kevin P. Le, Inana X. Schutze, Chan C. Heu, Roni J. Gross, Peter G. Fabrick, Joseph A. Rodriguez, Aiden M. Hull, Daniel Langhorst, Jeffrey A. Fabrick, Colin S. Brent","doi":"10.1002/arch.70053","DOIUrl":"https://doi.org/10.1002/arch.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>Mirid plant bugs (Hemiptera: Miridae), including <i>Lygus hesperus</i> (western tarnished plant bug), are key pests of numerous agricultural crops. While management of this pest relies heavily on chemical insecticides, the evolution of resistance and environmental concerns underscore the need for new and more effective approaches. Genetic-based strategies that target male fertiliy are currently being evaluated for population suppression. However, a lack of candidate gene targets with appropriate function, specifically in non-model species like <i>L. hesperus</i>, has hindered progress in the development and application of such approaches. Given their conserved role in stabilization of the flagella axoneme and association with sperm motility in many organisms, members of the <i>tektin</i> gene family represent logical targets for genetic-based sterilization. Here, we identified four homologs of the non-vertebrate <i>tektin</i> family from <i>L. hesperus</i> and used RNA interference-mediated knockdown to assess their roles in male fertility. Although transcription of the four <i>tektins</i> was predominantly in the testis, knockdown had negligible effects on either sperm abundance or male fertility. Our results suggest that tektins do not play a critical role in sperm fertilization of eggs in <i>L</i>. <i>hesperus</i> and are thus likely poor targets for genetic-based sterilization approaches in this species.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural Diversity of Adipokinetic Hormones in the Coleopteran Suborder Polyphaga (Excluding Cucujiformia)","authors":"Gerd Gäde, Simone König, Heather G. Marco","doi":"10.1002/arch.70049","DOIUrl":"10.1002/arch.70049","url":null,"abstract":"<p>Beetles are the largest animal group, in general. Phylogenetically, beetles belong to the order Coleoptera, the most species-rich of the Insecta. Coleoptera is divided into four suborders: Polyphaga, Adephaga, Archostemata, and Myxophaga. Specimens from the latter two are difficult to obtain, hence, we have focused our research into the adipokinetic hormone (AKH) peptide family on the former two suborders. Data on the Adephaga were concluded in 2017. The “core Polyphaga” consists of three series: Elateriformia, Staphyliniformia, and Cucujiformia; the latter was concluded in 2019. Here, we report on the AKH sequence(s) of 23 species of beetles from 4 families of Elateriformia, namely, the Buprestidae, Cantharidae, Elateridae, and Lampyridae; and 4 families of Staphyliniformia, namely, the Hydrophilidae, Silphidae, Lucanidae, and Scarabaeidae. Sequence elucidation by mass spectrometry or Edman degradation revealed 13 octapeptides: 5 are novel, 12 are beetle-specific and Schgr-AKH-II is produced in the basal Polyphaga (Elateriformia, in Hydrophilididae and Silphidae). Since Schgr-AKH-II is also found in Adephaga, this confirms the ancestral AKH of Coleoptera. The first change in sequence is recorded in Staphylinoidea with two different residues, notably, the switch from Phe<sup>4</sup> to Tyr<sup>4</sup>. Duplication of AKH peptides is first seen in Lucanidae, as well as the appearance of atypical AKH sequences, such as Phe<sup>2</sup>, Met<sup>4</sup>, Leu<sup>4</sup>, or Phe<sup>7</sup> encountered in the Scarabaeoidea. The vast majority of the pest beetles do not have beetle-specific AKHs or share the same AKH as nonharmful beetles. Ideas for finding a lead compound for green insecticides are discussed.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evidence for Transgenerational Immunity in Antiviral Immunity in Silkworm, Bombyx mori","authors":"Qiang Wang, Zengqing Huang, FeiFei Wang, Zhonghe Sun, Xiaoli Ju, Keping Chen","doi":"10.1002/arch.70041","DOIUrl":"10.1002/arch.70041","url":null,"abstract":"<div>\u0000 \u0000 <p>Transgenerational immune priming (TGIP) is a phenomenon in which parental exposure to pathogen infection enhances resistance to pathogens in their offspring. TGIP has been reported in several insects, including Lepidoptera, but it has not yet been documented in silkworms. In this study, we demonstrated the existence of TGIP in silkworms by exposing the parental generation to a low dose of <i>Bombyx mori</i> nucleopolyhedrovirus (BmNPV). Notably, when maternal or paternal-primed moths were mated to produce the F1 generation, the F1 generation larvae from both primed groups were more resistant to the BmNPV challenge than silkworm larvae with only maternal or paternal priming. Importantly, both maternal and paternal contributions to offspring immunity were essential for TGIP. However, due to the characteristics of the BmNPV itself, no within-generation immune responses were detected following BmNPV priming. Further analysis revealed that immune-related genes might play a role in mediating specific TGIP in silkworms after BmNPV priming. These results broaden our understanding of TGIP and the antiviral memory of insects in their offspring.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Design and Synthesis of Nano-dsRNA Nanoparticles Targeting Bombyx mori Acetyltransferase BmCBP and Its Impact on BmApoLp-III Expression","authors":"Zhan Luo, Yanmei Chen, Qinglong Kong, Yue Liu, Guowei Zu, Zhuo Jiang, Xucong Fu, Zhengying You, Zhengbing Lyu, Zuoming Nie","doi":"10.1002/arch.70051","DOIUrl":"10.1002/arch.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>As a key acetyltransferase, <i>Bombyx mori</i> cAMP response element-binding protein (CREB)-binding protein (BmCBP) plays an important role in post-translational modifications, affecting protein stability and immune response in silkworm; however, its mechanism of action remains unclear. In this study, we designed and synthesized Nano-dsRNA nanoparticles targeting <i>BmCBP</i> and validated their impact on BmApoLp-III protein expression. Using an <i>E. coli</i> expression system, we successfully synthesized nano-dsRNA targeting <i>BmCBP</i>, which significantly reduced <i>BmCBP</i> transcription level, thereby decreasing the acetylation and protein expression levels of BmApoLp-III. The knock-down of <i>BmCBP</i> by nano-dsRNA also increased the ubiquitination level of BmApoLp-III, suggesting a competitive relationship between acetylation and ubiquitination. These findings show that design and synthesis of efficient nano-dsRNA nanoparticles targeting <i>BmCBP</i> provide a novel tool and approach for studying gene expression and regulation in silkworm and other insects.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Drosophila as a Promising In Vivo Research Model for the Application and Development of Targeted Protein Inactivation Technologies","authors":"Natalia V. Dorogova, Svetlana A. Fedorova","doi":"10.1002/arch.70046","DOIUrl":"https://doi.org/10.1002/arch.70046","url":null,"abstract":"<div>\u0000 \u0000 <p>Technologies for controlled protein targeting allow the selective manipulations of proteins resulting in their degradation and/or loss of function. Over the past two decades, these technologies have overcome the limitations of genetic methods and have become powerful tools in biological research and the search for new therapeutic approaches to disease treatment. Various methods of protein degradation and inactivation have been successfully applied to a model organism such as <i>Drosophila melanogaster</i>. In this article, we overview the capabilities and prospects of the Drosophila in vivo model for testing and developing modern methods of controlled protein targeting, analyzing their efficacy at the organism level and solving fundamental biological problems.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differential Processing of Sucrose and Invert Syrup in Honey Bees","authors":"Ratko Pavlović, Biljana Dojnov, Marinela Šokarda Slavić, Marina Ristović, Miroslava Vujčić, Sanja Stojanović, Zoran Vujčić","doi":"10.1002/arch.70052","DOIUrl":"https://doi.org/10.1002/arch.70052","url":null,"abstract":"<div>\u0000 \u0000 <p>Feeding bees carbohydrates, as a substitute for nectar, has become essential in modern beekeeping. We compared the effects of 65% sucrose (SS) and 65% invert sugar (IS) syrups on the survival and enzyme activity of caged honey bees. Specifically, we analyzed glycosidase activity in the head and midgut of the bees and compared the composition of sucrose-based (SH) and invert sugar-based (IH) “honey” stored by the bees and collected from the comb. Glycosidase activity was similar in head in contrast to midgut where it was higher in IS fed bees, which appeared to be residual yeast β-fructofuranosidase activity. Fructose to glucose ratio in SH were 60.84/39.16 and a presence of some other sugars were detected, while ratio in IH were 48.49/51.51, almost exactly the same as in start fed (IS) (48.57/51.43). It has been demonstrated that glycosidase activity in IH was residual yeast β-fructofuranosidase activity. Zymogram detected α-amylase band in SH, in contrast to IH, which suggest that honey bees do not add amylase into IS. In contrast to SH, no crystallization occurred in IH. SS and IS densities were 1.23 and 1.24 g/mL, respectively, increasing to 1.35 g/mL in SH and 1.28 g/mL in IH after processing. This suggests that higher humidity and restricted cleansing flight make it harder for bees to remove excess water from IH, leading to increased midgut and hindgut weight, which correlates with higher mortality in the third week for the IS-fed group and fourth week for the SS-fed group.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to “Microcystin-LR-Induced Changes in Growth Performance, Intestinal Microbiota, and Lipid Metabolism of Black Soldier Fly Larvae (Hermetia illucens)”","authors":"","doi":"10.1002/arch.70047","DOIUrl":"https://doi.org/10.1002/arch.70047","url":null,"abstract":"<p>Gu, P., Ding, Y., Zhang, W., Yang, K., Zhang, Z., Ren, X., Su, H. and Miao, H. (2025), Microcystin-LR-Induced Changes in Growth Performance, Intestinal Microbiota, and Lipid Metabolism of Black Soldier Fly Larvae (<i>Hermetia illucens</i>). <i>Archives of Insect Biochemistry and Physiology,</i> 118: e70036. https://doi.org/10.1002/arch.70036.</p><p>In the paper by Gu et al. (2025), there was an error in the authors' affiliations. The order of the authors' affiliations should be:</p><p><sup>2</sup>Wuxi River and Lake Treatment and Water Resources Management Center, Wuxi, Jiangsu, China.</p><p><sup>3</sup>Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou, Jiangsu, China.</p><p>We apologize for this error.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survival, Movement, and Lifespan: Decoding the Roles of Patched-Related in Drosophila melanogaster","authors":"Cristina Parada, Daniel Prieto","doi":"10.1002/arch.70048","DOIUrl":"https://doi.org/10.1002/arch.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>Patched-related (Ptr) is a transmembrane protein implicated in developmental processes in <i>Drosophila melanogaster</i>, yet its precise role remains incompletely understood. Here, we use <i>Ptr</i><sup><i>23c</i></sup> null mutants to investigate the functional significance of Ptr through the entire life cycle monitoring survival during embryonic, larval, pupal and adult development, and studying larval locomotion and muscle structure. We report that <i>Ptr</i><sup><i>23c</i></sup> larvae displayed impaired hatching, indicative of defective embryonic development. Moreover, mutant larvae exhibited reduced mobility and lethargy, suggesting a potential involvement of Ptr in neuromuscular function. Morphological analysis of somatic muscles in mutant larvae revealed enlarged cell nuclei. Despite high preadult mortality, a subset of <i>Ptr</i><sup><i>23c</i></sup> mutant adults display an unexpected extension in lifespan compared to controls, implicating Ptr in the regulation of longevity. Our findings provide critical insights into the multifaceted role of Ptr in <i>Drosophila</i> development, highlighting its contributions to post-embryonic survival, neuromuscular function, and lifespan regulation. This study underscores the significance of exploring broader genetic networks to unravel the complexities of developmental processes.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CRISPR/Cas9 Mediated Editing of Bdtektin1 Gene Induces Sterility in Male Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae)","authors":"Chikmagalur Nagaraja Bhargava, Karuppannasamy Ashok, Sanjay Kumar Pradhan, Sampath Kumar, Maligeppagol Manamohan, Anil Rai, Ramasamy Asokan","doi":"10.1002/arch.70043","DOIUrl":"https://doi.org/10.1002/arch.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>The Oriental fruit fly, <i>Bactrocera dorsalis</i> (<i>B. dorsalis</i>) is a highly invasive, widely distributed notorious pest restricting global fruit trade immensely. There are several approaches to managing this pest, still require newer approaches. In this regard, recently a novel approach called precision-guided sterile insect technique (pgSIT) is gaining momentum in inducing both female sex elimination or sex conversion and male sterility at one go. Developing a species-specific pgSIT system requires validation of targets such as sex determination and spermatogenesis genes. In this regard, <i>B. dorsalis</i> is highly amenable for area-wide pest management and in the present study, we have validated the loss-of-function of the spermatogenesis-related gene, <i>tektin1</i> using the CRISPR/Cas9 ribonucleoprotein (RNP) complex. This gene was cloned from the local isolate of <i>B. dorsalis</i> and two promising single guide RNAs (sgRNAs) were designed and validated through in vitro restriction analysis. Injection of the RNP complex (sgRNA + Cas9 protein) into the G0 embryo resulted in three adult males carrying mutations at the target site. The phenotype of the mutants was determined through crossing studies, namely, △1♂ × WT ♀, △2♂ × WT ♀, △3♂ × WT ♀, and WT ♂ × WT ♀ and that showed hatching rates of 0%, 11.70%, 0%, and 45.12%, respectively. The mutant males had more nonviable sperm as compared to control. This study underscores the pivotal role of the <i>Bdtektin1</i> gene for male fertility and is a promising candidate for further development of pgSIT system for <i>B. dorsalis</i>.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Humidity and the Role of Visual Cues During Feeding on Green/Brown Color Polyphenism in Locusta migratoria","authors":"Keiryu Hirota, Ryo Watanabe, Ryohei Sugahara","doi":"10.1002/arch.70044","DOIUrl":"https://doi.org/10.1002/arch.70044","url":null,"abstract":"<p>The regulation of body color in locusts comprises phase polyphenism, green/brown polyphenism, and homochromy. Humidity was believed to control green/brown color polyphenism in locust species. However, recent findings indicate that humidity has minimal influence on this polyphenism in <i>Schistocerca gregaria</i> nymphs reared in isolation. This study investigated the effects of humidity and visual stimuli on <i>Locusta migratoria</i> nymphs reared in isolation. When <i>L. migratoria</i> nymphs were fed either <i>Bromus catharticus</i> or <i>Dactylis glomerata</i> leaves only during the dark period, the proportions of green nymphs at the 5th instar were comparable. Increased humidity (through addition of moist cotton) induced more green morphs under a 16-h dark/8-h light cycle, suggesting that humidity is involved in green/brown polyphenism in <i>L. migratoria</i>. However, these nymphs were not sensitive to humidity regarding this polyphenism under a 23-h dark/1-h light cycle, suggesting that sensitivity is related to visual cues. The daily overlap between food availability and light periods for 12 h resulted in a higher proportion of green morphs than a daily overlap for only 4 h, suggesting that such an overlap affects green/brown polyphenism in this species. Although we were unable to assess the effect of the total food availability period on polyphenism in this study, we confirmed that at least (1) humidity and (2) visual cues during feeding are associated with green/brown polyphenism in <i>L. migratoria</i>. These regulations may have ecological significance for this species, allowing them to phenotypically adjust to the seasonal and spatial variation in their environment, increasing their chances of survival.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}