Archives of Insect Biochemistry and Physiology最新文献_第2页

Thermal Stress and Its Effects on the Gut Microbiome of Parthenium Beetles 热应激及其对 Parthenium 甲虫肠道微生物组的影响

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-08 DOI: 10.1002/arch.70058

Parinita Singh, Prema Haldhar, Tamal Das, Gyaneshwer Chaubey, Munesh Kumar Gupta, Bhupendra Kumar

{"title":"Thermal Stress and Its Effects on the Gut Microbiome of Parthenium Beetles","authors":"Parinita Singh, Prema Haldhar, Tamal Das, Gyaneshwer Chaubey, Munesh Kumar Gupta, Bhupendra Kumar","doi":"10.1002/arch.70058","DOIUrl":"https://doi.org/10.1002/arch.70058","url":null,"abstract":"<div>\u0000 \u0000 The gut microbiota plays a vital role in nutrient and energy utilization, as well as in the host's ability to adapt its immune system to environmental changes. As a biological control agent for the invasive Parthenium weed, the Parthenium beetle Zygogramma bicolorata (Z. bicolorata) Pallister is often exposed to fluctuating temperatures, which may induce stress in its natural habitat. This study utilized 16S amplicon sequencing to explore the impact of temperature stress on the gut microbiome of Z. bicolorata under cold (15°C), control (27°C), and hot (35°C) conditions. A total of 11 bacterial phyla and 149 genera were identified, with Firmicutes, Proteobacteria, and Cyanobacteria being the most abundant. Temperature treatments significantly influenced the diversity of the gut microbiota, as evidenced by alpha diversity measures. Principal coordinate analysis further revealed substantial variations in microbiome composition across the different temperature conditions. Additionally, PICRUSt2 analysis suggested that the gut microbiota is linked to metagenomic functions related to amino acid and carbohydrate transport, inorganic ion metabolism, and cellular processes. Our findings suggest that thermal stress alters the gut microbiome of Parthenium beetles, offering new insights into how these beetles may have ecologically adapted to temperature fluctuations, while also highlighting the potential role of gut microbes in maintaining beetle health under environmental stress.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water Transport and Enzyme Recycling in Tenebrio molitor Midgut: Insights From Transcriptomics, Proteomics, and In Vivo Inhibition Assays 黄粉虫中肠的水运输和酶循环：来自转录组学、蛋白质组学和体内抑制分析的见解

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-08 DOI: 10.1002/arch.70059

Ignacio G. Barroso, Clelia Ferreira, Walter R. Terra

{"title":"Water Transport and Enzyme Recycling in Tenebrio molitor Midgut: Insights From Transcriptomics, Proteomics, and In Vivo Inhibition Assays","authors":"Ignacio G. Barroso, Clelia Ferreira, Walter R. Terra","doi":"10.1002/arch.70059","DOIUrl":"https://doi.org/10.1002/arch.70059","url":null,"abstract":"<div>\u0000 \u0000 The low excretory rates of secreted digestive enzymes, such as trypsins, in insect species with peritrophic membranes led to the hypothesis of ectoperitrophic countercurrent water fluxes causing enzyme recycling. The midgut water flux model of Tenebrio molitor (T. molitor) is revisited and supported by in vivo experiments. Sequences from proteins putatively involved in water transport were retrieved from the T. molitor transcriptome by Blast and analyzed using bioinformatics tools. Gene expression of selected proteins was determined in three midgut sections (anterior, AM; middle, MM; posterior, PM) by RNA-seq, and transporter proteins were verified in microvillar-membrane-enriched midgut samples by proteomics. Genes encoding three cation chloride cotransporters (CCC) and four aquaporins were expressed in the midgut. TmNaCCC2, TmPrip, and TmEglp1 showed higher expression in the front half, while TmKCC, TmNKCC1, TmDrip, and TmEglp2 were more highly expressed in the back half. However, only TmNaCCC2 was found by proteomics. Midgut water fluxes were quantified by feeding T. molitor larvae with nonabsorbable dye and measuring its concentration along the midgut. The results suggest water absorption in AM and secretion in MM and PM, potentially caused by TmNaCCC2 and TmPrip in AM, and TmKCC and TmDrip in PM, whereas MM serves as a transition region. Larvae fed on furosemide, an NKCC and KCC inhibitor, showed altered midgut water fluxes, resulting in higher trypsin excretion into the hindgut, thus reinforcing the hypothesis of a countercurrent water flux generated by CCCs powering enzyme recycling in insect midguts.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNAi-Mediated Knockdown of Tektins Does Not Affect Male Fertility in Lygus hesperus rnai介导的tektin蛋白敲低不影响雄性羊尾草的生育能力

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-01 DOI: 10.1002/arch.70053

J. Joe Hull, Kevin P. Le, Inana X. Schutze, Chan C. Heu, Roni J. Gross, Peter G. Fabrick, Joseph A. Rodriguez, Aiden M. Hull, Daniel Langhorst, Jeffrey A. Fabrick, Colin S. Brent

{"title":"RNAi-Mediated Knockdown of Tektins Does Not Affect Male Fertility in Lygus hesperus","authors":"J. Joe Hull, Kevin P. Le, Inana X. Schutze, Chan C. Heu, Roni J. Gross, Peter G. Fabrick, Joseph A. Rodriguez, Aiden M. Hull, Daniel Langhorst, Jeffrey A. Fabrick, Colin S. Brent","doi":"10.1002/arch.70053","DOIUrl":"https://doi.org/10.1002/arch.70053","url":null,"abstract":"<div>\u0000 \u0000 Mirid plant bugs (Hemiptera: Miridae), including Lygus hesperus (western tarnished plant bug), are key pests of numerous agricultural crops. While management of this pest relies heavily on chemical insecticides, the evolution of resistance and environmental concerns underscore the need for new and more effective approaches. Genetic-based strategies that target male fertiliy are currently being evaluated for population suppression. However, a lack of candidate gene targets with appropriate function, specifically in non-model species like L. hesperus, has hindered progress in the development and application of such approaches. Given their conserved role in stabilization of the flagella axoneme and association with sperm motility in many organisms, members of the tektin gene family represent logical targets for genetic-based sterilization. Here, we identified four homologs of the non-vertebrate tektin family from L. hesperus and used RNA interference-mediated knockdown to assess their roles in male fertility. Although transcription of the four tektins was predominantly in the testis, knockdown had negligible effects on either sperm abundance or male fertility. Our results suggest that tektins do not play a critical role in sperm fertilization of eggs in L. hesperus and are thus likely poor targets for genetic-based sterilization approaches in this species.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Diversity of Adipokinetic Hormones in the Coleopteran Suborder Polyphaga (Excluding Cucujiformia) 鞘翅目多角亚目（不包括葫芦形目）脂肪动力激素的结构多样性。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-21 DOI: 10.1002/arch.70049

Gerd Gäde, Simone König, Heather G. Marco

{"title":"Structural Diversity of Adipokinetic Hormones in the Coleopteran Suborder Polyphaga (Excluding Cucujiformia)","authors":"Gerd Gäde, Simone König, Heather G. Marco","doi":"10.1002/arch.70049","DOIUrl":"10.1002/arch.70049","url":null,"abstract":"Beetles are the largest animal group, in general. Phylogenetically, beetles belong to the order Coleoptera, the most species-rich of the Insecta. Coleoptera is divided into four suborders: Polyphaga, Adephaga, Archostemata, and Myxophaga. Specimens from the latter two are difficult to obtain, hence, we have focused our research into the adipokinetic hormone (AKH) peptide family on the former two suborders. Data on the Adephaga were concluded in 2017. The “core Polyphaga” consists of three series: Elateriformia, Staphyliniformia, and Cucujiformia; the latter was concluded in 2019. Here, we report on the AKH sequence(s) of 23 species of beetles from 4 families of Elateriformia, namely, the Buprestidae, Cantharidae, Elateridae, and Lampyridae; and 4 families of Staphyliniformia, namely, the Hydrophilidae, Silphidae, Lucanidae, and Scarabaeidae. Sequence elucidation by mass spectrometry or Edman degradation revealed 13 octapeptides: 5 are novel, 12 are beetle-specific and Schgr-AKH-II is produced in the basal Polyphaga (Elateriformia, in Hydrophilididae and Silphidae). Since Schgr-AKH-II is also found in Adephaga, this confirms the ancestral AKH of Coleoptera. The first change in sequence is recorded in Staphylinoidea with two different residues, notably, the switch from Phe4 to Tyr4. Duplication of AKH peptides is first seen in Lucanidae, as well as the appearance of atypical AKH sequences, such as Phe2, Met4, Leu4, or Phe7 encountered in the Scarabaeoidea. The vast majority of the pest beetles do not have beetle-specific AKHs or share the same AKH as nonharmful beetles. Ideas for finding a lead compound for green insecticides are discussed.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for Transgenerational Immunity in Antiviral Immunity in Silkworm, Bombyx mori 桑蚕抗病毒免疫中跨代免疫的证据

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-21 DOI: 10.1002/arch.70041

Qiang Wang, Zengqing Huang, FeiFei Wang, Zhonghe Sun, Xiaoli Ju, Keping Chen

{"title":"Evidence for Transgenerational Immunity in Antiviral Immunity in Silkworm, Bombyx mori","authors":"Qiang Wang, Zengqing Huang, FeiFei Wang, Zhonghe Sun, Xiaoli Ju, Keping Chen","doi":"10.1002/arch.70041","DOIUrl":"10.1002/arch.70041","url":null,"abstract":"<div>\u0000 \u0000 Transgenerational immune priming (TGIP) is a phenomenon in which parental exposure to pathogen infection enhances resistance to pathogens in their offspring. TGIP has been reported in several insects, including Lepidoptera, but it has not yet been documented in silkworms. In this study, we demonstrated the existence of TGIP in silkworms by exposing the parental generation to a low dose of Bombyx mori nucleopolyhedrovirus (BmNPV). Notably, when maternal or paternal-primed moths were mated to produce the F1 generation, the F1 generation larvae from both primed groups were more resistant to the BmNPV challenge than silkworm larvae with only maternal or paternal priming. Importantly, both maternal and paternal contributions to offspring immunity were essential for TGIP. However, due to the characteristics of the BmNPV itself, no within-generation immune responses were detected following BmNPV priming. Further analysis revealed that immune-related genes might play a role in mediating specific TGIP in silkworms after BmNPV priming. These results broaden our understanding of TGIP and the antiviral memory of insects in their offspring.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and Synthesis of Nano-dsRNA Nanoparticles Targeting Bombyx mori Acetyltransferase BmCBP and Its Impact on BmApoLp-III Expression 家蚕乙酰转移酶BmCBP纳米dsrna的设计合成及其对BmApoLp-III表达的影响

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-21 DOI: 10.1002/arch.70051

Zhan Luo, Yanmei Chen, Qinglong Kong, Yue Liu, Guowei Zu, Zhuo Jiang, Xucong Fu, Zhengying You, Zhengbing Lyu, Zuoming Nie

{"title":"Design and Synthesis of Nano-dsRNA Nanoparticles Targeting Bombyx mori Acetyltransferase BmCBP and Its Impact on BmApoLp-III Expression","authors":"Zhan Luo, Yanmei Chen, Qinglong Kong, Yue Liu, Guowei Zu, Zhuo Jiang, Xucong Fu, Zhengying You, Zhengbing Lyu, Zuoming Nie","doi":"10.1002/arch.70051","DOIUrl":"10.1002/arch.70051","url":null,"abstract":"<div>\u0000 \u0000 As a key acetyltransferase, Bombyx mori cAMP response element-binding protein (CREB)-binding protein (BmCBP) plays an important role in post-translational modifications, affecting protein stability and immune response in silkworm; however, its mechanism of action remains unclear. In this study, we designed and synthesized Nano-dsRNA nanoparticles targeting BmCBP and validated their impact on BmApoLp-III protein expression. Using an E. coli expression system, we successfully synthesized nano-dsRNA targeting BmCBP, which significantly reduced BmCBP transcription level, thereby decreasing the acetylation and protein expression levels of BmApoLp-III. The knock-down of BmCBP by nano-dsRNA also increased the ubiquitination level of BmApoLp-III, suggesting a competitive relationship between acetylation and ubiquitination. These findings show that design and synthesis of efficient nano-dsRNA nanoparticles targeting BmCBP provide a novel tool and approach for studying gene expression and regulation in silkworm and other insects.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila as a Promising In Vivo Research Model for the Application and Development of Targeted Protein Inactivation Technologies 果蝇是靶向蛋白失活技术应用和发展的有前途的体内研究模型

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-17 DOI: 10.1002/arch.70046

Natalia V. Dorogova, Svetlana A. Fedorova

引用次数: 0

Differential Processing of Sucrose and Invert Syrup in Honey Bees 蔗糖和转化糖浆在蜜蜂体内的差别加工

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-16 DOI: 10.1002/arch.70052

Ratko Pavlović, Biljana Dojnov, Marinela Šokarda Slavić, Marina Ristović, Miroslava Vujčić, Sanja Stojanović, Zoran Vujčić

{"title":"Differential Processing of Sucrose and Invert Syrup in Honey Bees","authors":"Ratko Pavlović, Biljana Dojnov, Marinela Šokarda Slavić, Marina Ristović, Miroslava Vujčić, Sanja Stojanović, Zoran Vujčić","doi":"10.1002/arch.70052","DOIUrl":"https://doi.org/10.1002/arch.70052","url":null,"abstract":"<div>\u0000 \u0000 Feeding bees carbohydrates, as a substitute for nectar, has become essential in modern beekeeping. We compared the effects of 65% sucrose (SS) and 65% invert sugar (IS) syrups on the survival and enzyme activity of caged honey bees. Specifically, we analyzed glycosidase activity in the head and midgut of the bees and compared the composition of sucrose-based (SH) and invert sugar-based (IH) “honey” stored by the bees and collected from the comb. Glycosidase activity was similar in head in contrast to midgut where it was higher in IS fed bees, which appeared to be residual yeast β-fructofuranosidase activity. Fructose to glucose ratio in SH were 60.84/39.16 and a presence of some other sugars were detected, while ratio in IH were 48.49/51.51, almost exactly the same as in start fed (IS) (48.57/51.43). It has been demonstrated that glycosidase activity in IH was residual yeast β-fructofuranosidase activity. Zymogram detected α-amylase band in SH, in contrast to IH, which suggest that honey bees do not add amylase into IS. In contrast to SH, no crystallization occurred in IH. SS and IS densities were 1.23 and 1.24 g/mL, respectively, increasing to 1.35 g/mL in SH and 1.28 g/mL in IH after processing. This suggests that higher humidity and restricted cleansing flight make it harder for bees to remove excess water from IH, leading to increased midgut and hindgut weight, which correlates with higher mortality in the third week for the IS-fed group and fourth week for the SS-fed group.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Microcystin-LR-Induced Changes in Growth Performance, Intestinal Microbiota, and Lipid Metabolism of Black Soldier Fly Larvae (Hermetia illucens)” 修正“微囊藻毒素- lr诱导的黑兵蝇幼虫生长性能、肠道微生物群和脂质代谢的变化”

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-11 DOI: 10.1002/arch.70047

引用次数: 0

Survival, Movement, and Lifespan: Decoding the Roles of Patched-Related in Drosophila melanogaster 生存、运动和寿命：解码黑腹果蝇中补丁相关基因的作用

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-11 DOI: 10.1002/arch.70048

Cristina Parada, Daniel Prieto

{"title":"Survival, Movement, and Lifespan: Decoding the Roles of Patched-Related in Drosophila melanogaster","authors":"Cristina Parada, Daniel Prieto","doi":"10.1002/arch.70048","DOIUrl":"https://doi.org/10.1002/arch.70048","url":null,"abstract":"<div>\u0000 \u0000 Patched-related (Ptr) is a transmembrane protein implicated in developmental processes in Drosophila melanogaster, yet its precise role remains incompletely understood. Here, we use Ptr23c null mutants to investigate the functional significance of Ptr through the entire life cycle monitoring survival during embryonic, larval, pupal and adult development, and studying larval locomotion and muscle structure. We report that Ptr23c larvae displayed impaired hatching, indicative of defective embryonic development. Moreover, mutant larvae exhibited reduced mobility and lethargy, suggesting a potential involvement of Ptr in neuromuscular function. Morphological analysis of somatic muscles in mutant larvae revealed enlarged cell nuclei. Despite high preadult mortality, a subset of Ptr23c mutant adults display an unexpected extension in lifespan compared to controls, implicating Ptr in the regulation of longevity. Our findings provide critical insights into the multifaceted role of Ptr in Drosophila development, highlighting its contributions to post-embryonic survival, neuromuscular function, and lifespan regulation. This study underscores the significance of exploring broader genetic networks to unravel the complexities of developmental processes.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0