Archives of Insect Biochemistry and Physiology最新文献

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Unravelling the Role of Insulin-Like Peptide Genes in Bombyx mori: Potential Key Regulators of Insect Metabolism 揭示家蚕胰岛素样肽基因的作用:昆虫代谢的潜在关键调节因子
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-16 DOI: 10.1002/arch.70054
Creaminar D. Shira, Kanmoni Malakar, Bidyadhar Das
{"title":"Unravelling the Role of Insulin-Like Peptide Genes in Bombyx mori: Potential Key Regulators of Insect Metabolism","authors":"Creaminar D. Shira,&nbsp;Kanmoni Malakar,&nbsp;Bidyadhar Das","doi":"10.1002/arch.70054","DOIUrl":"https://doi.org/10.1002/arch.70054","url":null,"abstract":"<div>\u0000 \u0000 <p>Understanding gene expression in specific tissues and their modulation under environmental stimuli, such as nutritional deficiency, reveals the key physiological regulatory mechanisms of an organism. This study examined the tissue-specific expression of insulin-like peptide (ILP) genes (<i>BmX</i> and <i>BmZ</i>) in <i>Bombyx mori</i> larvae and their responses to hyperglycaemia, food deprivation and hormonal (20-hydroxyecdysone and bovine insulin) treatments. mRNA expression levels of <i>BmX</i> and <i>BmZ</i> were analyzed in the brain, fat body, midgut and ovary. The results revealed that <i>BmX</i> was highly expressed in the fat body, while both genes were abundant in the ovary. Hyperglycaemia increased <i>BmX</i> mRNA expression level in the midgut (3.07-fold) and brain (7.53-fold), while <i>BmZ</i> mRNA expression level was increased in all tissues except the midgut. Nutrient deficiency upregulated <i>BmX</i> mRNA expression level (1.36-fold) in the fat body while reducing it (−0.53-fold) in the midgut. Food deprivation progressively increased (0.77-fold at 24 h and 2.34-fold at 72 h) <i>BmX</i> mRNA expression level in the fat body, while both <i>BmX</i> and <i>BmZ</i> transcripts declined in the midgut. Insulin suppressed <i>BmX</i> (−0.25-fold) and <i>BmZ</i> (−0.91-fold) mRNA expression levels in food-deprived larvae in the fat body, whereas 20E consistently downregulated <i>BmX, BmZ</i>, and <i>BmInR</i> (insulin receptor) mRNA expression levels in all the conditions. These findings revealed the complex interaction of gene expression, tissue specificity, and environmental factors in <i>B. mori</i> larvae and provided insights into adaptive responses to nutritional stress and hormonal regulation in the insect with potential applications in sericulture and agricultural biotechnology.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Histone and N-Terminal Acetyltransferases Required for Reproduction and Embryonic Development of Yellow Fever Mosquito, Aedes aegypti 埃及伊蚊繁殖和胚胎发育所需组蛋白和n端乙酰转移酶的鉴定
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-16 DOI: 10.1002/arch.70055
Sundararajan Balasubramani, Subba Reddy Palli
{"title":"Identification of Histone and N-Terminal Acetyltransferases Required for Reproduction and Embryonic Development of Yellow Fever Mosquito, Aedes aegypti","authors":"Sundararajan Balasubramani,&nbsp;Subba Reddy Palli","doi":"10.1002/arch.70055","DOIUrl":"https://doi.org/10.1002/arch.70055","url":null,"abstract":"<div>\u0000 \u0000 <p>Histone acetylation levels maintained by histone acetyltransferases (HATs) and histone deacetylases play important roles in maintaining local chromatin accessibility and expression of genes that regulate many biological processes, including development and reproduction. N-terminal acetylation of proteins catalyzed by N-terminal acetyltransferases (NATs) also regulates gene expression. We identified 25 HATs/NATs genes in the yellow fever mosquito, <i>Aedes aegypti</i>, and investigated their function in female reproduction using RNA interference (RNAi). Among the HATs/NATs studied, the knockdown of AANAT1 (Arylamine N-acetyltransferase), NAA40 (N-alpha-acetyltransferase 40), NAA80 (N-alpha-acetyltransferase 80), KAT7 (Histone lysine acetyltransferase 7), ACNAT (Acyl-CoA N-acyltransferase), and MCM3AP (Minichromosome maintenance complex component 3 associated protein) significantly reduced egg laying and caused severe problems in oocyte development compared to that in control insects injected with dsGFP. Gene expression analysis using RT-qPCR revealed that vitellogenin and its receptor genes are downregulated in mosquitoes injected with dsAANAT1, dsNAA40, dsNAA80, dsKAT7, dsACNAT, and dsMCM3AP compared to that in control animals. Also, the knockdown of HATs/NATs genes ATAT1 (Alpha-tubulin N-acetyltransferase 1), AANAT1, TAFIID (Transcription initiation factor TFIID subunit 1), HATB (Histone acetyltransferase type B) and NAT9 (N-acetyltransferase 9) decreased more than 50% egg hatch by blocking embryonic development. These results suggest that the acetylation of proteins, especially histones mediated by NATs and HATs, plays an important role in regulating female reproduction and embryonic development of <i>Ae. aegypti</i>.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thermal Stress and Its Effects on the Gut Microbiome of Parthenium Beetles 热应激及其对 Parthenium 甲虫肠道微生物组的影响
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-08 DOI: 10.1002/arch.70058
Parinita Singh, Prema Haldhar, Tamal Das, Gyaneshwer Chaubey, Munesh Kumar Gupta, Bhupendra Kumar
{"title":"Thermal Stress and Its Effects on the Gut Microbiome of Parthenium Beetles","authors":"Parinita Singh,&nbsp;Prema Haldhar,&nbsp;Tamal Das,&nbsp;Gyaneshwer Chaubey,&nbsp;Munesh Kumar Gupta,&nbsp;Bhupendra Kumar","doi":"10.1002/arch.70058","DOIUrl":"https://doi.org/10.1002/arch.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>The gut microbiota plays a vital role in nutrient and energy utilization, as well as in the host's ability to adapt its immune system to environmental changes. As a biological control agent for the invasive Parthenium weed, the Parthenium beetle <i>Zygogramma bicolorata</i> (<i>Z. bicolorata</i>) Pallister is often exposed to fluctuating temperatures, which may induce stress in its natural habitat. This study utilized 16S amplicon sequencing to explore the impact of temperature stress on the gut microbiome of <i>Z. bicolorata</i> under cold (15°C), control (27°C), and hot (35°C) conditions. A total of 11 bacterial phyla and 149 genera were identified, with Firmicutes, Proteobacteria, and Cyanobacteria being the most abundant. Temperature treatments significantly influenced the diversity of the gut microbiota, as evidenced by alpha diversity measures. Principal coordinate analysis further revealed substantial variations in microbiome composition across the different temperature conditions. Additionally, PICRUSt2 analysis suggested that the gut microbiota is linked to metagenomic functions related to amino acid and carbohydrate transport, inorganic ion metabolism, and cellular processes. Our findings suggest that thermal stress alters the gut microbiome of Parthenium beetles, offering new insights into how these beetles may have ecologically adapted to temperature fluctuations, while also highlighting the potential role of gut microbes in maintaining beetle health under environmental stress.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Water Transport and Enzyme Recycling in Tenebrio molitor Midgut: Insights From Transcriptomics, Proteomics, and In Vivo Inhibition Assays 黄粉虫中肠的水运输和酶循环:来自转录组学、蛋白质组学和体内抑制分析的见解
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-08 DOI: 10.1002/arch.70059
Ignacio G. Barroso, Clelia Ferreira, Walter R. Terra
{"title":"Water Transport and Enzyme Recycling in Tenebrio molitor Midgut: Insights From Transcriptomics, Proteomics, and In Vivo Inhibition Assays","authors":"Ignacio G. Barroso,&nbsp;Clelia Ferreira,&nbsp;Walter R. Terra","doi":"10.1002/arch.70059","DOIUrl":"https://doi.org/10.1002/arch.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>The low excretory rates of secreted digestive enzymes, such as trypsins, in insect species with peritrophic membranes led to the hypothesis of ectoperitrophic countercurrent water fluxes causing enzyme recycling. The midgut water flux model of <i>Tenebrio molitor</i> (<i>T. molitor</i>) is revisited and supported by in vivo experiments. Sequences from proteins putatively involved in water transport were retrieved from the <i>T. molitor</i> transcriptome by Blast and analyzed using bioinformatics tools. Gene expression of selected proteins was determined in three midgut sections (anterior, AM; middle, MM; posterior, PM) by RNA-seq, and transporter proteins were verified in microvillar-membrane-enriched midgut samples by proteomics. Genes encoding three cation chloride cotransporters (CCC) and four aquaporins were expressed in the midgut. <i>TmNaCCC2</i>, <i>TmPrip</i>, and <i>TmEglp1</i> showed higher expression in the front half, while <i>TmKCC</i>, <i>TmNKCC1, TmDrip</i>, and <i>TmEglp2</i> were more highly expressed in the back half. However, only <i>TmNaCCC2</i> was found by proteomics. Midgut water fluxes were quantified by feeding <i>T. molitor</i> larvae with nonabsorbable dye and measuring its concentration along the midgut. The results suggest water absorption in AM and secretion in MM and PM, potentially caused by <i>TmNaCCC2</i> and <i>TmPrip</i> in AM, and <i>TmKCC</i> and <i>TmDrip</i> in PM, whereas MM serves as a transition region. Larvae fed on furosemide, an NKCC and KCC inhibitor, showed altered midgut water fluxes, resulting in higher trypsin excretion into the hindgut, thus reinforcing the hypothesis of a countercurrent water flux generated by CCCs powering enzyme recycling in insect midguts.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RNAi-Mediated Knockdown of Tektins Does Not Affect Male Fertility in Lygus hesperus rnai介导的tektin蛋白敲低不影响雄性羊尾草的生育能力
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-04-01 DOI: 10.1002/arch.70053
J. Joe Hull, Kevin P. Le, Inana X. Schutze, Chan C. Heu, Roni J. Gross, Peter G. Fabrick, Joseph A. Rodriguez, Aiden M. Hull, Daniel Langhorst, Jeffrey A. Fabrick, Colin S. Brent
{"title":"RNAi-Mediated Knockdown of Tektins Does Not Affect Male Fertility in Lygus hesperus","authors":"J. Joe Hull,&nbsp;Kevin P. Le,&nbsp;Inana X. Schutze,&nbsp;Chan C. Heu,&nbsp;Roni J. Gross,&nbsp;Peter G. Fabrick,&nbsp;Joseph A. Rodriguez,&nbsp;Aiden M. Hull,&nbsp;Daniel Langhorst,&nbsp;Jeffrey A. Fabrick,&nbsp;Colin S. Brent","doi":"10.1002/arch.70053","DOIUrl":"https://doi.org/10.1002/arch.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>Mirid plant bugs (Hemiptera: Miridae), including <i>Lygus hesperus</i> (western tarnished plant bug), are key pests of numerous agricultural crops. While management of this pest relies heavily on chemical insecticides, the evolution of resistance and environmental concerns underscore the need for new and more effective approaches. Genetic-based strategies that target male fertiliy are currently being evaluated for population suppression. However, a lack of candidate gene targets with appropriate function, specifically in non-model species like <i>L. hesperus</i>, has hindered progress in the development and application of such approaches. Given their conserved role in stabilization of the flagella axoneme and association with sperm motility in many organisms, members of the <i>tektin</i> gene family represent logical targets for genetic-based sterilization. Here, we identified four homologs of the non-vertebrate <i>tektin</i> family from <i>L. hesperus</i> and used RNA interference-mediated knockdown to assess their roles in male fertility. Although transcription of the four <i>tektins</i> was predominantly in the testis, knockdown had negligible effects on either sperm abundance or male fertility. Our results suggest that tektins do not play a critical role in sperm fertilization of eggs in <i>L</i>. <i>hesperus</i> and are thus likely poor targets for genetic-based sterilization approaches in this species.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural Diversity of Adipokinetic Hormones in the Coleopteran Suborder Polyphaga (Excluding Cucujiformia) 鞘翅目多角亚目(不包括葫芦形目)脂肪动力激素的结构多样性。
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-21 DOI: 10.1002/arch.70049
Gerd Gäde, Simone König, Heather G. Marco
{"title":"Structural Diversity of Adipokinetic Hormones in the Coleopteran Suborder Polyphaga (Excluding Cucujiformia)","authors":"Gerd Gäde,&nbsp;Simone König,&nbsp;Heather G. Marco","doi":"10.1002/arch.70049","DOIUrl":"10.1002/arch.70049","url":null,"abstract":"<p>Beetles are the largest animal group, in general. Phylogenetically, beetles belong to the order Coleoptera, the most species-rich of the Insecta. Coleoptera is divided into four suborders: Polyphaga, Adephaga, Archostemata, and Myxophaga. Specimens from the latter two are difficult to obtain, hence, we have focused our research into the adipokinetic hormone (AKH) peptide family on the former two suborders. Data on the Adephaga were concluded in 2017. The “core Polyphaga” consists of three series: Elateriformia, Staphyliniformia, and Cucujiformia; the latter was concluded in 2019. Here, we report on the AKH sequence(s) of 23 species of beetles from 4 families of Elateriformia, namely, the Buprestidae, Cantharidae, Elateridae, and Lampyridae; and 4 families of Staphyliniformia, namely, the Hydrophilidae, Silphidae, Lucanidae, and Scarabaeidae. Sequence elucidation by mass spectrometry or Edman degradation revealed 13 octapeptides: 5 are novel, 12 are beetle-specific and Schgr-AKH-II is produced in the basal Polyphaga (Elateriformia, in Hydrophilididae and Silphidae). Since Schgr-AKH-II is also found in Adephaga, this confirms the ancestral AKH of Coleoptera. The first change in sequence is recorded in Staphylinoidea with two different residues, notably, the switch from Phe<sup>4</sup> to Tyr<sup>4</sup>. Duplication of AKH peptides is first seen in Lucanidae, as well as the appearance of atypical AKH sequences, such as Phe<sup>2</sup>, Met<sup>4</sup>, Leu<sup>4</sup>, or Phe<sup>7</sup> encountered in the Scarabaeoidea. The vast majority of the pest beetles do not have beetle-specific AKHs or share the same AKH as nonharmful beetles. Ideas for finding a lead compound for green insecticides are discussed.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence for Transgenerational Immunity in Antiviral Immunity in Silkworm, Bombyx mori 桑蚕抗病毒免疫中跨代免疫的证据
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-21 DOI: 10.1002/arch.70041
Qiang Wang, Zengqing Huang, FeiFei Wang, Zhonghe Sun, Xiaoli Ju, Keping Chen
{"title":"Evidence for Transgenerational Immunity in Antiviral Immunity in Silkworm, Bombyx mori","authors":"Qiang Wang,&nbsp;Zengqing Huang,&nbsp;FeiFei Wang,&nbsp;Zhonghe Sun,&nbsp;Xiaoli Ju,&nbsp;Keping Chen","doi":"10.1002/arch.70041","DOIUrl":"10.1002/arch.70041","url":null,"abstract":"<div>\u0000 \u0000 <p>Transgenerational immune priming (TGIP) is a phenomenon in which parental exposure to pathogen infection enhances resistance to pathogens in their offspring. TGIP has been reported in several insects, including Lepidoptera, but it has not yet been documented in silkworms. In this study, we demonstrated the existence of TGIP in silkworms by exposing the parental generation to a low dose of <i>Bombyx mori</i> nucleopolyhedrovirus (BmNPV). Notably, when maternal or paternal-primed moths were mated to produce the F1 generation, the F1 generation larvae from both primed groups were more resistant to the BmNPV challenge than silkworm larvae with only maternal or paternal priming. Importantly, both maternal and paternal contributions to offspring immunity were essential for TGIP. However, due to the characteristics of the BmNPV itself, no within-generation immune responses were detected following BmNPV priming. Further analysis revealed that immune-related genes might play a role in mediating specific TGIP in silkworms after BmNPV priming. These results broaden our understanding of TGIP and the antiviral memory of insects in their offspring.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design and Synthesis of Nano-dsRNA Nanoparticles Targeting Bombyx mori Acetyltransferase BmCBP and Its Impact on BmApoLp-III Expression 家蚕乙酰转移酶BmCBP纳米dsrna的设计合成及其对BmApoLp-III表达的影响
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-21 DOI: 10.1002/arch.70051
Zhan Luo, Yanmei Chen, Qinglong Kong, Yue Liu, Guowei Zu, Zhuo Jiang, Xucong Fu, Zhengying You, Zhengbing Lyu, Zuoming Nie
{"title":"Design and Synthesis of Nano-dsRNA Nanoparticles Targeting Bombyx mori Acetyltransferase BmCBP and Its Impact on BmApoLp-III Expression","authors":"Zhan Luo,&nbsp;Yanmei Chen,&nbsp;Qinglong Kong,&nbsp;Yue Liu,&nbsp;Guowei Zu,&nbsp;Zhuo Jiang,&nbsp;Xucong Fu,&nbsp;Zhengying You,&nbsp;Zhengbing Lyu,&nbsp;Zuoming Nie","doi":"10.1002/arch.70051","DOIUrl":"10.1002/arch.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>As a key acetyltransferase, <i>Bombyx mori</i> cAMP response element-binding protein (CREB)-binding protein (BmCBP) plays an important role in post-translational modifications, affecting protein stability and immune response in silkworm; however, its mechanism of action remains unclear. In this study, we designed and synthesized Nano-dsRNA nanoparticles targeting <i>BmCBP</i> and validated their impact on BmApoLp-III protein expression. Using an <i>E. coli</i> expression system, we successfully synthesized nano-dsRNA targeting <i>BmCBP</i>, which significantly reduced <i>BmCBP</i> transcription level, thereby decreasing the acetylation and protein expression levels of BmApoLp-III. The knock-down of <i>BmCBP</i> by nano-dsRNA also increased the ubiquitination level of BmApoLp-III, suggesting a competitive relationship between acetylation and ubiquitination. These findings show that design and synthesis of efficient nano-dsRNA nanoparticles targeting <i>BmCBP</i> provide a novel tool and approach for studying gene expression and regulation in silkworm and other insects.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Drosophila as a Promising In Vivo Research Model for the Application and Development of Targeted Protein Inactivation Technologies 果蝇是靶向蛋白失活技术应用和发展的有前途的体内研究模型
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-17 DOI: 10.1002/arch.70046
Natalia V. Dorogova, Svetlana A. Fedorova
{"title":"Drosophila as a Promising In Vivo Research Model for the Application and Development of Targeted Protein Inactivation Technologies","authors":"Natalia V. Dorogova,&nbsp;Svetlana A. Fedorova","doi":"10.1002/arch.70046","DOIUrl":"https://doi.org/10.1002/arch.70046","url":null,"abstract":"<div>\u0000 \u0000 <p>Technologies for controlled protein targeting allow the selective manipulations of proteins resulting in their degradation and/or loss of function. Over the past two decades, these technologies have overcome the limitations of genetic methods and have become powerful tools in biological research and the search for new therapeutic approaches to disease treatment. Various methods of protein degradation and inactivation have been successfully applied to a model organism such as <i>Drosophila melanogaster</i>. In this article, we overview the capabilities and prospects of the Drosophila in vivo model for testing and developing modern methods of controlled protein targeting, analyzing their efficacy at the organism level and solving fundamental biological problems.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential Processing of Sucrose and Invert Syrup in Honey Bees 蔗糖和转化糖浆在蜜蜂体内的差别加工
IF 1.5 4区 农林科学
Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-16 DOI: 10.1002/arch.70052
Ratko Pavlović, Biljana Dojnov, Marinela Šokarda Slavić, Marina Ristović, Miroslava Vujčić, Sanja Stojanović, Zoran Vujčić
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