Archives of Insect Biochemistry and Physiology最新文献_第3页

Metarhizium anisopliae JEF-197 Loses Glucose Metabolism in Surviving Japanese Pine Sawyer Beetle Against the Fungal Pathogen

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-10 DOI: 10.1002/arch.70020

Gahyeon Song, Mi Rong Lee, Jong Cheol Kim, Jae Su Kim

{"title":"Metarhizium anisopliae JEF-197 Loses Glucose Metabolism in Surviving Japanese Pine Sawyer Beetle Against the Fungal Pathogen","authors":"Gahyeon Song, Mi Rong Lee, Jong Cheol Kim, Jae Su Kim","doi":"10.1002/arch.70020","DOIUrl":"https://doi.org/10.1002/arch.70020","url":null,"abstract":"<div>\u0000 \u0000 Japanese pine sawyer beetle (JPSB), Monochamus alternatus is a forest insect pest with damaging to pine trees through vectoring plant-parasitic nematodes. In our previous work, the entomopathogenic Metarhizium anisopliae JEF-197 was effective in controlling JPSB adults. However some of JPSB adults survived well even against the fungal treatment. Now here in this work, we analyzed the transcriptome of JEF-197 from the fungus-treated JPSB adults which still survived in 8 days after the treatment. The day was determined based on the lethal time 50 (LT50) in our previous study. As a control, JEF-197 was cultured on 1/4SDA for 8 days. The plate-cultured JEF-197 transcripts were used for building-up an index in the abundance analysis using kallisto to investigate the gene regulation. Additionally, transcripts from the JEF-197-treated PSB were analyzed to find possible fungal transcripts to enlarge the index of abundance analysis. In the following differentially expressed gene (DEG) analysis, most JEF-197 transcripts showed significant down-regulations in JPSB adults 8 days after treatment, which were presented as clustering heatmap, PCA, MA and Volcano plots. The GO enrichment analysis showed similar results, in which most of pathways were significantly suppressed. Metabolic and biosynthesis metabolisms were most dominantly downregulated pathways. Particularly, many genes of glucose metabolisms were significantly suppressed, including genes for glycolysis, TCA, ATP & nucleotide synthesis, and glycogen & chitin production. This work suggests that JEF-197 lost its own glucose metabolism in the survived JPSB adults, and the survival could be involved in the active and continuous host defense mechanisms. It gives us questions what factors would be involved in the different response of individual to the fungal treatment and what happens if live and dead hosts were pooled in RNA-sequencing.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Head-Specific Transcriptomic Study Reveals Key Regulatory Pathways for Winter Diapause in the Mosquito Culex pipiens

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-03 DOI: 10.1002/arch.70032

Prabin Dhungana, Xueyan Wei, David S. Kang, Cheolho Sim

{"title":"A Head-Specific Transcriptomic Study Reveals Key Regulatory Pathways for Winter Diapause in the Mosquito Culex pipiens","authors":"Prabin Dhungana, Xueyan Wei, David S. Kang, Cheolho Sim","doi":"10.1002/arch.70032","DOIUrl":"10.1002/arch.70032","url":null,"abstract":"<div>\u0000 \u0000 The primary vector of the West Nile virus, Culex pipiens, undergoes reproductive dormancy during the adverse winter season. While our current understanding has mainly focused on cellular signals and phenotypic shifts occurring at a global scale during diapause, information on tissue-specific transcriptomic changes remains limited. This knowledge gap is a major challenge in interpreting the regulatory mechanisms at the tissue level. To address this, the present work utilized RNA-seq technology to investigate the transcriptional changes in the head that house the brain and crucial endocrinal organs such as corpora allata. We obtained RNA samples from the heads of diapausing and nondiapausing female mosquitoes at two specific time intervals, ZT0 and ZT16, and then subjected them to sequencing. Our results revealed differences in differentially expressed genes between diapause and non-diapause at ZT0 and ZT16, highlighting the phenotypic and diel variations in gene expression. We also selected twelve genes associated with the diapause phenotype and examined the transcript abundance at six different time points over 24 h. qRT-PCR analysis showed similar up- and downregulation of transcripts between the diapause and nondiapause phenotypes thus validating the results of RNA-seq. In summary, our study identified new genes with phenotypic and diel differentiation in their expression, potentially linking photoperiod to seasonal reproductive dormancy in insects. The newly presented information will significantly advance our understanding of head-specific genes crucial for insect diapause.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon Quantum Dot Nanoparticles Enhance the Efficacy of Spodoptera littoralis Nucleopolyhedrovirus Suspoemulsion

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-03 DOI: 10.1002/arch.70027

Ali Mehrvar, Solmaz Ghanbari, Gökhan Söylemezoğlu, Umut Toprak

{"title":"Carbon Quantum Dot Nanoparticles Enhance the Efficacy of Spodoptera littoralis Nucleopolyhedrovirus Suspoemulsion","authors":"Ali Mehrvar, Solmaz Ghanbari, Gökhan Söylemezoğlu, Umut Toprak","doi":"10.1002/arch.70027","DOIUrl":"10.1002/arch.70027","url":null,"abstract":"This study evaluates the efficacy of Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) and laboratory-synthesized carbon quantum dot nanoparticles (CQDNPs) against the second instar Spodoptera littoralis larvae under laboratory and greenhouse conditions. Individually, both SpliNPV and CQDNPs exhibited substantial lethality (91.6% and 83.3% at 1 × 108 OBs/ml and 700 mg/ml, respectively) (p < 0.05). The LC50 values were 1.88 × 105 OB/ml and 434.2 mg/mL, and the LT50 values were 8.9 and 9.8 days, respectively. Four LC-based combined treatments demonstrated significant additive effects, with the SpliNPV (LC50) + CQDNPs (LC25) combination achieving the optimum effect with a mortality rate of 86.3% and an LT50 value of 6.6 days, leading to its selection for the suspoemulsion nanoparticle (SENP) formulation. The SENP formulation displayed superior performance, achieving the highest mortality rates and fastest killing times across all environments: 89.0% in laboratory conditions, 83.3% on eggplant plants, and 76.6% on pepper plants. In contrast, the suspoemulsion (SE) and unformulated (UF) formulations showed lower efficacy, emphasizing the importance of formulation in enhancing the biological activity of SpliNPV. The LT50 values further supported these findings, with the SENP formulation demonstrating the shortest LT50 values, indicating faster lethality. A significant decrease in CHS-B, IIM2, PER3, REPAT14, and CDA1 expression was observed, particularly in the combined CQDNPs + SpliNPV treatment, while API expression increased significantly. These findings highlight the potential of nanoparticle-enhanced formulations like SENP, and integrating CQDNPs with SpliNPV can significantly enhance pest control efficacy.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk Assessment of Cyclaniliprole Resistance in Peach-Potato Aphid Myzus persicae: Laboratory Selection, Inheritance, and Cross-Resistance Patterns

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-26 DOI: 10.1002/arch.70031

Pingzhuo Liang, Zihao Zhang, Congai Zhen, Dapeng Li, Shenhang Cheng, Ren Li, Lei Zhang

{"title":"Risk Assessment of Cyclaniliprole Resistance in Peach-Potato Aphid Myzus persicae: Laboratory Selection, Inheritance, and Cross-Resistance Patterns","authors":"Pingzhuo Liang, Zihao Zhang, Congai Zhen, Dapeng Li, Shenhang Cheng, Ren Li, Lei Zhang","doi":"10.1002/arch.70031","DOIUrl":"10.1002/arch.70031","url":null,"abstract":"<div>\u0000 \u0000 Cyclaniliprole, a type of the third-generation anthranilic diamide insecticide, was mainly used for management of various pests. Myzus persicae (Hemiptera: Aphididae), known as the peach-potato aphid, is an economically essential pest with worldwide distribution. However, the risk assessment of cyclaniliprole in M. persicae is unclear. The cyclaniliprole risk assessment in M. persicae showed 2.56-fold resistance to cyclaniliprole after 26 generation selection in comparison to the initial susceptible population. The cross-resistance experiment revealed that the low cross-resistance to imidacloprid (4.2-fold) in the cyclaniliprole-selected strain was observed when comparing to the susceptible population. Realized heritability (h2) of cyclaniliprole resistance was 0.0362. When mean slope = 2.217 and h2 = 0.0362, then 31–69 generations would be required for an increase of LC50s with ten times at 90%–50% selection intensity. The fecundity (the number of offspring per female) of the cyclaniliprole-selected strain had no significant difference with the susceptible population. The mRNA expression of the target gene ryanodine receptor was significantly enhanced in the cyclaniliprole-selected strain. The absence of fitness costs, the minimal resistance risk, and very low levels of cross-resistance in the cyclaniliprole-selected strain provide strong support for designing the effective management strategies against M. persicae.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activin β Is Critical for Larval-Pupal Transition in the 28 Spotted Lady Beetle Henosepilachna vigintioctopunctata 激活素β对28只斑瓢虫幼虫-蛹转化至关重要。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-21 DOI: 10.1002/arch.70025

Kai-Yun Fu, Feng Chen, Lin Jin, Guo-Qing Li

{"title":"Activin β Is Critical for Larval-Pupal Transition in the 28 Spotted Lady Beetle Henosepilachna vigintioctopunctata","authors":"Kai-Yun Fu, Feng Chen, Lin Jin, Guo-Qing Li","doi":"10.1002/arch.70025","DOIUrl":"10.1002/arch.70025","url":null,"abstract":"<div>\u0000 \u0000 The activin cascade is activated when a pair of extracellular ligand (Myoglianin, Myo; Activin β, Actβ; Dawdle, Daw) binds to two pairs of transforming growth factor β (TGF) serine-threonine receptor kinases, TGF-β type I (Baboon, Babo) and II receptors. However, the roles of activin way have not well been explored in non-Drosophilid insects. In the present paper, we compared the functions of Activin β (Actβ) ligand and receptor isoform BaboB in post-embryonic development in a defoliating ladybird Henosepilachna vigintioctopunctata. RNA interference (RNAi) for Hvactβ but not Hvbabob upregulated juvenile hormone signal, reduced ecdysone pathway and impaired larval-pupal transformation. The arresting Hvactβ RNAi larvae formed pupa-specific black markings below the larval exuviae. Thus, the impairment of metamorphosis may be caused by failing to complete ecdysis behavior due to nonfunctional muscles. Consistently, larval body sizes were smaller and adult appendages were shorter in the Hvactβ RNAi larvae, in contrast to those in the Hvbabob depleted beetles. Conversely, knockdown of Hvbabob but not Hvactβ changed the pigmentation of adult elytra. Our results suggest that Actβ exerts regulative roles in JH production, ecdysteroidogenesis and organ remodeling, thus contributing to modulate the larva-pupa-adult transformation, through a BaboB independent way in H. vigintioctopunctata.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apprehending siRNA Machinery and Gene Silencing in Brinjal Shoot and Fruit Borer, Leucinodes orbonalis 茄子茎、果螟siRNA机制及基因沉默研究。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-21 DOI: 10.1002/arch.70029

N. Veeramanikanta Reddy, T. C. Suman, Gracy R. Gandhi, Jyoti Pathak, Y. K. Yadu, T. Venkatesan, Satya Nand Sushil

{"title":"Apprehending siRNA Machinery and Gene Silencing in Brinjal Shoot and Fruit Borer, Leucinodes orbonalis","authors":"N. Veeramanikanta Reddy, T. C. Suman, Gracy R. Gandhi, Jyoti Pathak, Y. K. Yadu, T. Venkatesan, Satya Nand Sushil","doi":"10.1002/arch.70029","DOIUrl":"10.1002/arch.70029","url":null,"abstract":"<div>\u0000 \u0000 RNA interference (RNAi) technology is widely used in gene functional studies and has been shown to be a promising next generation alternative for insect pest management. To understand the efficiency of RNAi machinery in Leucinodes orbonalis (L. orbonalis) Guenee, a destructive pest of eggplant, core RNAi pathway genes Argonaute-2, Dicer-2, Loquacious, and Sid-1 were mined from the transcriptome and characterized. The transcript abundance of these genes was studied after exposure to exogenous double-stranded RNA (dsRNA). Domain structure analysis revealed that these genes have conserved domains required for the definite protein function in the siRNA pathway. The protein sequences when subjected to phylogenetic analysis showed a close relation with homologs obtained from Ostrinia sp. The insects fed with dsRNA designed for vacuolar sorting protein SNF7 gene showed significant downregulation at 48 h post treatment and about 79% larval mortality. The expression study of genes showed a significant spike in transcript abundance of Dicer-2, Argonatute-2, and downregulation of Loquacious at 24 and 48 h post dsRNA exposure. The results on siRNA machinery genes expression and target gene knockdown implies L. orbonalis has an ample response to exogenous dsRNA.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZnT35C Maintains Zinc Homeostasis to Regulate Spermatogenesis in Drosophila Testis ZnT35C维持锌稳态调节果蝇睾丸精子发生。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-21 DOI: 10.1002/arch.70017

Jiayu He, Yang Fang, Long Zhao, Ying Su

{"title":"ZnT35C Maintains Zinc Homeostasis to Regulate Spermatogenesis in Drosophila Testis","authors":"Jiayu He, Yang Fang, Long Zhao, Ying Su","doi":"10.1002/arch.70017","DOIUrl":"10.1002/arch.70017","url":null,"abstract":"<div>\u0000 \u0000 Zinc homeostasis contributes significantly to numerous physiological processes. Drosophila ZnT35C protein, a zinc transporter encoded by CG3994, is chiefly located on the cell membrane and facilitates the transport of zinc from the cytoplasm to the extracellular space to sustain zinc homeostasis within the organism. Previous studies about ZnT35C have involved diverse structures such as the Malpighian tubules, adult brain, and sensory nervous system. Nonetheless, the role of ZnT35C in Drosophila spermatogenesis remained unclear. In our study, we discovered that ZnT35C plays a pivotal role in Drosophila spermatogenesis. Its knockdown resulted in sperm loss and male infertility. When ZnT35C was knocked down in cyst cells, zinc was concentrated within cyst cells, inhibiting the proper development of germ cells and thereby causing the incapacity of flies to generate mature sperms. Zinc supplementation can effectively rescue this failure of spermatogenesis. Our research outcomes suggest that ZnT35C, through modulating the zinc environment within the testes, impacts the male fertility of Drosophila, occupying a crucial position in the spermatogenesis process.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Feeding and Pupation Bioassays to Assess the Effects of Insecticidal and Repellent Treatments on Aethina tumida Larval Development and Pupation Success 优化取食和化蛹生物测定方法，评估杀虫驱避处理对肿腹小蜂幼虫发育和化蛹成功的影响。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-21 DOI: 10.1002/arch.70023

Morgan A. Roth, Aaron D. Gross

{"title":"Optimizing Feeding and Pupation Bioassays to Assess the Effects of Insecticidal and Repellent Treatments on Aethina tumida Larval Development and Pupation Success","authors":"Morgan A. Roth, Aaron D. Gross","doi":"10.1002/arch.70023","DOIUrl":"10.1002/arch.70023","url":null,"abstract":"European honey bee (Apis mellifera) colonies are an ideal host to the invasive beetle Aethina tumida, providing a nutrient rich environment that is protected from the elements and facilitates beetle reproduction. Although various management techniques and chemical treatments for A. tumida have been developed, understanding the efficacy of these treatments and techniques is limited. Throughout this study, several methods for impairing A. tumida development and delivering insecticidal, repellent, or antifungal treatments were examined. A series of A. tumida larval feeding bioassays developed and optimized feeding gel pellet for delivery of insecticidal treatments, revealing that A. tumida larvae are sensitive to the two common in-hive varroa mite (Varroa destructor) treatments: coumaphos (EC50 = 25.6 ppm) and tau-fluvalinate (EC50 = 21.2 ppm). Feeding bioassays also demonstrated that A. tumida were more sensitive to the pyrethroid compounds permethrin (EC50 = 3.37 ppm), deltamethrin (EC50 = 2.69 ppm), and bifenthrin (EC50 = 0.365 ppm), which have been previously used to control this beetle. Feeding bioassays also revealed that the antifungal drug Amphotericin B was palatable to A. tumida larvae via feeding, but was also injected into A. tumida larvae and adults. Two types of pupation bioassays were also developed to test the effects of several insecticidal and repellent treatments on pupation burrowing and pupation success. Overall, this work details specific toxicity information regarding common insecticidal treatments found in the apiary setting study and provides groundwork and methods for testing insecticidal compounds on A. tumida larvae in in the future.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the Effects of Three Carbohydrate Feeds on the Health of Apis mellifera by Transcriptome Analysis 通过转录组分析了解三种碳水化合物饲料对蜜蜂健康的影响。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-21 DOI: 10.1002/arch.70026

Ting Xiong, Daohao Xie, Zhitao Li, Zhiyue Yang, Kun Dong, Minghua Yang, Yahui Li

{"title":"Understanding the Effects of Three Carbohydrate Feeds on the Health of Apis mellifera by Transcriptome Analysis","authors":"Ting Xiong, Daohao Xie, Zhitao Li, Zhiyue Yang, Kun Dong, Minghua Yang, Yahui Li","doi":"10.1002/arch.70026","DOIUrl":"10.1002/arch.70026","url":null,"abstract":"<div>\u0000 \u0000 At present, there is no clear consensus on the impact of carbohydrate feeds on bee colony health, and comprehensive research and evaluation in this context is lacking. To comprehensively and objectively examine the health status of honeybees after consuming those carbohydrates from multiple perspectives, experimental techniques, including high-throughput sequencing of the transcriptome, proboscis extension reflex (PER), and measuring bee growth parameters were employed. This study showed that compared with honey, feeding high fructose syrup (HFS) resulted in a decrease in the survival rate and body weight of bees, while sucrose decreased the learning and memory ability of bees. After feeding on honey, the main antimicrobial peptides including abaecin, apidaecin1, hymenoptin, and defensin in bees, are all upregulated in expression. The 14 DEGs significantly enriched in the axonal regeneration pathway were all downregulated in the sucrose group and HFS group. This study demonstrated that the expression of multiple genes involved in oxidative phosphorylation was downregulated in bees fed with HFS, moreover, HFS also affected the biosynthesis of unsaturated fatty acids. These effects may lead to energy and metabolic disorders (including fatty acids), thereby inhibiting the growth and development of bees. Sucrose can decrease the learning and memory ability of bees, which may be due to the downregulation of genes related to learning and memory in the axonal regeneration pathway. Honey can upregulate antimicrobial peptides and other immune-related proteins, activating the bee's immune system and boosting bees' immunity to pathogens.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of IRES-Dependent Translation by Rocaglamide A Increases the Replication and Virulence of Cricket Paralysis Virus in Lepidopteran Insect Cells Rocaglamide A刺激ires依赖性翻译增加鳞翅目昆虫细胞中蟋蟀麻痹病毒的复制和毒力。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-01-21 DOI: 10.1002/arch.70028

Dulce Santos, Vasiliki-Maria Christopoulou, Clauvis Nji Tizi Taning, Socratis Avgeris, Adamantia Papadopoulou, Dimitris Kletsas, Gerassimos E. Voutsinas, Vasiliki Labropoulou, Luc Swevers

{"title":"Stimulation of IRES-Dependent Translation by Rocaglamide A Increases the Replication and Virulence of Cricket Paralysis Virus in Lepidopteran Insect Cells","authors":"Dulce Santos, Vasiliki-Maria Christopoulou, Clauvis Nji Tizi Taning, Socratis Avgeris, Adamantia Papadopoulou, Dimitris Kletsas, Gerassimos E. Voutsinas, Vasiliki Labropoulou, Luc Swevers","doi":"10.1002/arch.70028","DOIUrl":"10.1002/arch.70028","url":null,"abstract":"<div>\u0000 \u0000 The discovery that infections of viruses are pervasive among insects has considerable potential for future applications, such as new strategies for pest control through the manipulation of virus-host interactions. However, few studies can be found that aim to minimize (for beneficial insects) or maximize (for pests) virus impact or virulence. Viruses generally employ molecular mechanisms that deviate from the cells’ to increase their replication efficiency and to avoid the immune response. In this research, a screening system is presented for the detection of molecules that interfere with the internal ribosomal entry site (IRES) of Cricket paralysis virus (Dicistroviridae) which has been well characterized in previous research. Over-expression and RNAi experiments identified the importance of eIF4A, a component of the cap-dependent translation initiation complex, to modify the activity of IRES-mediated translation. Application of Rocaglamide A (RocA), a natural product from Aglaia plants and inhibitor of eIF4A, resulted in strong stimulation of IRES-mediated translation in reporter assays as well as increased CrPV genome replication and virion production in lepidopteran Hi5 cells. At 100 nM of RocA, dsRNA molecules accumulated in infected cells, corresponding to full-length genome (9.5 kb) and a smaller fragment (0.8 kb) with unknown function. Treatment of silkworm larvae with RocA by injection or topically was highly toxic while no strong stimulation of CrPV infection could be observed. The prospect of the use of rocaglamates as insecticides and enhancers of CrPV infection is discussed together with its potential impact on mammalian cells.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0