Archives of Insect Biochemistry and Physiology最新文献_第3页

CRISPR/Cas9 Mediated Editing of Bdtektin1 Gene Induces Sterility in Male Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) CRISPR/Cas9介导的Bdtektin1基因编辑诱导雄性桔小实蝇不育（双翅目：蝗科）

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-03-11 DOI: 10.1002/arch.70043

Chikmagalur Nagaraja Bhargava, Karuppannasamy Ashok, Sanjay Kumar Pradhan, Sampath Kumar, Maligeppagol Manamohan, Anil Rai, Ramasamy Asokan

{"title":"CRISPR/Cas9 Mediated Editing of Bdtektin1 Gene Induces Sterility in Male Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae)","authors":"Chikmagalur Nagaraja Bhargava, Karuppannasamy Ashok, Sanjay Kumar Pradhan, Sampath Kumar, Maligeppagol Manamohan, Anil Rai, Ramasamy Asokan","doi":"10.1002/arch.70043","DOIUrl":"https://doi.org/10.1002/arch.70043","url":null,"abstract":"<div>\u0000 \u0000 The Oriental fruit fly, Bactrocera dorsalis (B. dorsalis) is a highly invasive, widely distributed notorious pest restricting global fruit trade immensely. There are several approaches to managing this pest, still require newer approaches. In this regard, recently a novel approach called precision-guided sterile insect technique (pgSIT) is gaining momentum in inducing both female sex elimination or sex conversion and male sterility at one go. Developing a species-specific pgSIT system requires validation of targets such as sex determination and spermatogenesis genes. In this regard, B. dorsalis is highly amenable for area-wide pest management and in the present study, we have validated the loss-of-function of the spermatogenesis-related gene, tektin1 using the CRISPR/Cas9 ribonucleoprotein (RNP) complex. This gene was cloned from the local isolate of B. dorsalis and two promising single guide RNAs (sgRNAs) were designed and validated through in vitro restriction analysis. Injection of the RNP complex (sgRNA + Cas9 protein) into the G0 embryo resulted in three adult males carrying mutations at the target site. The phenotype of the mutants was determined through crossing studies, namely, △1♂ × WT ♀, △2♂ × WT ♀, △3♂ × WT ♀, and WT ♂ × WT ♀ and that showed hatching rates of 0%, 11.70%, 0%, and 45.12%, respectively. The mutant males had more nonviable sperm as compared to control. This study underscores the pivotal role of the Bdtektin1 gene for male fertility and is a promising candidate for further development of pgSIT system for B. dorsalis.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Humidity and the Role of Visual Cues During Feeding on Green/Brown Color Polyphenism in Locusta migratoria 湿度和进食时视觉线索对蝗虫绿/棕色多色性的影响

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-25 DOI: 10.1002/arch.70044

Keiryu Hirota, Ryo Watanabe, Ryohei Sugahara

{"title":"The Effect of Humidity and the Role of Visual Cues During Feeding on Green/Brown Color Polyphenism in Locusta migratoria","authors":"Keiryu Hirota, Ryo Watanabe, Ryohei Sugahara","doi":"10.1002/arch.70044","DOIUrl":"https://doi.org/10.1002/arch.70044","url":null,"abstract":"The regulation of body color in locusts comprises phase polyphenism, green/brown polyphenism, and homochromy. Humidity was believed to control green/brown color polyphenism in locust species. However, recent findings indicate that humidity has minimal influence on this polyphenism in Schistocerca gregaria nymphs reared in isolation. This study investigated the effects of humidity and visual stimuli on Locusta migratoria nymphs reared in isolation. When L. migratoria nymphs were fed either Bromus catharticus or Dactylis glomerata leaves only during the dark period, the proportions of green nymphs at the 5th instar were comparable. Increased humidity (through addition of moist cotton) induced more green morphs under a 16-h dark/8-h light cycle, suggesting that humidity is involved in green/brown polyphenism in L. migratoria. However, these nymphs were not sensitive to humidity regarding this polyphenism under a 23-h dark/1-h light cycle, suggesting that sensitivity is related to visual cues. The daily overlap between food availability and light periods for 12 h resulted in a higher proportion of green morphs than a daily overlap for only 4 h, suggesting that such an overlap affects green/brown polyphenism in this species. Although we were unable to assess the effect of the total food availability period on polyphenism in this study, we confirmed that at least (1) humidity and (2) visual cues during feeding are associated with green/brown polyphenism in L. migratoria. These regulations may have ecological significance for this species, allowing them to phenotypically adjust to the seasonal and spatial variation in their environment, increasing their chances of survival.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insect Resistance to Insecticides: Causes, Mechanisms, and Exploring Potential Solutions 昆虫对杀虫剂的抗药性：原因、机制和潜在解决方案探索

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-25 DOI: 10.1002/arch.70045

Jiyun Liang, Feng Xiao, James Ojo, Wu Hai Chao, Bilal Ahmad, Aleena Alam, Sohail Abbas, Mogeda M. Abdelhafez, Nadeemur Rahman, Khalid Ali Khan, Hamed A. Ghramh, Jamin Ali, Rizhao Chen

{"title":"Insect Resistance to Insecticides: Causes, Mechanisms, and Exploring Potential Solutions","authors":"Jiyun Liang, Feng Xiao, James Ojo, Wu Hai Chao, Bilal Ahmad, Aleena Alam, Sohail Abbas, Mogeda M. Abdelhafez, Nadeemur Rahman, Khalid Ali Khan, Hamed A. Ghramh, Jamin Ali, Rizhao Chen","doi":"10.1002/arch.70045","DOIUrl":"https://doi.org/10.1002/arch.70045","url":null,"abstract":"<div>\u0000 \u0000 Insecticides play a crucial role as the primary means of controlling agricultural pests, preventing significant damage to crops. However, the misuse of these insecticides has led to the development of resistance in insect pests against major classes of these chemicals. The emergence of resistance poses a serious threat, especially when alternative options for crop protection are limited for farmers. Addressing this challenge and developing new, effective, and sustainable pest management approaches is not merely essential but also critically important. In the absence of alternative solutions, understanding the root causes behind the development of resistance in insects becomes a critical necessity. Without this understanding, the formulation of effective approaches to combat resistance remains elusive. With insecticides playing a vital role in global food security and public health, understanding and mitigating resistance are paramount. Given the growing concern over insect resistance to insecticides, this review addresses a crucial research gap by thoroughly examining the causes, mechanisms, and potential solutions. The review examines factors driving resistance, such as evolutionary pressure and excessive pesticide use, and provides a detailed analysis of mechanisms, including detoxifying enzyme overproduction and target site mutations. Providing an analysis of potential solutions, it discusses integrated pest management, strategic insecticide rotation, and the use of new pest control technologies and biological agents. Emphasizing the urgency of a multifaceted approach, the review provides a concise roadmap for sustainable pest management, guiding future research and applications.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Feeding on Insect Larvae Treated With Pesticide on the Biological and Biochemical Characteristics of Arma chinensis 取食农药处理的昆虫幼虫对中华绒螯蟹生物学和生化特性的影响

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-23 DOI: 10.1002/arch.70042

Hui Zheng, Hailin Cai, Yunfei Mo, Xihui Guo, Wei Wang, Zhongxia Yang

{"title":"Effects of Feeding on Insect Larvae Treated With Pesticide on the Biological and Biochemical Characteristics of Arma chinensis","authors":"Hui Zheng, Hailin Cai, Yunfei Mo, Xihui Guo, Wei Wang, Zhongxia Yang","doi":"10.1002/arch.70042","DOIUrl":"https://doi.org/10.1002/arch.70042","url":null,"abstract":"<div>\u0000 \u0000 The effects of feeding on insect larvae treated with pesticide on the biological and biochemical characteristics of Arma chinensis were investigated. In the F0 generation, the developmental duration and preoviposition period were shortened, the average number of eggs laid was reduced, and the net reproductive rate and mean generation time were significantly lower in the treatment group compared to those in the control group. The average number of eggs laid by the F1 generation of A. chinensis was lower in the treatment group. The intrinsic rates of increase, finite rates of increase and net reproductive rates were significantly lower in the F1 generation in the treatment group than those in the control group. The activities of CarE and GST were significantly induced, while the activity of AChE was inhibited in the A. chinensis in the treatment group. The activities of trypsin and THL were significantly induced in the treatment group, while the activity of LPS was not significantly changed. At the population level, Enterococcaceae were cultured only from the intestines of the A. chinensis in the treatment group. At the genus level, in addition to Serratia, Yokenella and Klebsiella, which were consistent with the control group, the genus Enterococcus was also cultured in the treatment group. When controlling P. rapae in the field, pesticides should be reasonably selected, and an appropriate time should be chosen for the release of A. chinensis to reduce the impact on natural enemies and to achieve synergistic chemical and biological control.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Potential Immunostimulatory Effects of Cryptomeria japonica Leaf Essential Oil on Honey Bees (Apis mellifera) 柳杉叶精油对蜜蜂免疫刺激作用的初步研究

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-18 DOI: 10.1002/arch.70040

Hao-Yung Wang, Ying-Yu Chen, Chin-Jung Liu, Shih-Wei Huang, Shang-Tse Ho

{"title":"Evaluating the Potential Immunostimulatory Effects of Cryptomeria japonica Leaf Essential Oil on Honey Bees (Apis mellifera)","authors":"Hao-Yung Wang, Ying-Yu Chen, Chin-Jung Liu, Shih-Wei Huang, Shang-Tse Ho","doi":"10.1002/arch.70040","DOIUrl":"https://doi.org/10.1002/arch.70040","url":null,"abstract":"<div>\u0000 \u0000 This study investigated the effects of Cryptomeria japonica leaf essential oil (CjLEO) on honey bee health, focusing on both toxicity and gene expression modulation. Initial toxicity assessments revealed that high concentrations of CjLEO (75 and 100 ppm) were lethal to honey bees, resulting in complete mortality within a short period. Conversely, a lower concentration of 10 ppm exhibited no significant toxic effects, prompting further investigation into its sublethal impacts. Transcriptome analysis via next-generation sequencing demonstrated that CjLEO at 10 ppm induced significant changes in honey bee gene expression compared to the control group. Principal component analysis (PCA) and differential gene expression (DEG) analysis identified more than 9,000 genes, with notable upregulation of immune-related genes, including hymenoptaecin, abaecin, and apidaecin1. Gene ontology (GO) enrichment analysis indicated that these differentially expressed genes were primarily associated with immune responses, such as defense and innate immune pathways. The chemical composition of CjLEO, characterized by GC–MS, identified 16 compounds, with major components including α-pinene, elemol, α-eudesmol, and kaur-16-ene. These compounds are known for their antimicrobial properties, which likely contribute to the observed immunomodulatory effects. CjLEO at a concentration of 10 ppm enhances honey bee immunity without exhibiting significant toxicity, positioning it a promising candidate for improving honey bee resilience against pathogens. Future research should investigate the mechanisms of immune activation and optimize application methods for practical beekeeping, aiming to improve colony health while reducing dependence on synthetic chemicals.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Control of Social Experience-Dependent Changes in Locomotor Activity in Drosophila melanogaster Males 雄性黑腹果蝇运动活动社会经验依赖性变化的遗传控制

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-18 DOI: 10.1002/arch.70022

Julia V. Bragina, Anna A. Goncharova, Natalia G. Besedina, Larisa V. Danilenkova, Elena A. Kamysheva, Nikolai G. Kamyshev

{"title":"Genetic Control of Social Experience-Dependent Changes in Locomotor Activity in Drosophila melanogaster Males","authors":"Julia V. Bragina, Anna A. Goncharova, Natalia G. Besedina, Larisa V. Danilenkova, Elena A. Kamysheva, Nikolai G. Kamyshev","doi":"10.1002/arch.70022","DOIUrl":"https://doi.org/10.1002/arch.70022","url":null,"abstract":"<div>\u0000 \u0000 In animals, social experience plays an important role in the adaptive modification of behavior. Previous social experience changes locomotor activity in Drosophila melanogaster. In females, suppression of locomotion is observed only when flies are in aggregations, but males retain a reduced level of locomotor activity up to 5 days after being isolated from the group. The mechanisms underlying such behavioral plasticity still largely are unknown. In this study, we aimed to identify new candidate genes involved in the social experience-dependent modification of locomotor activity. We tested the effect of social experience on spontaneous locomotor activity in various mutant males, including those with impaired learning and memory, circadian rhythms, some biochemical pathways, and sensory systems. The results of the present study indicate that the biogenic amines and olfactory perception appear to play key roles in social experience-induced changes in locomotor activity. Also, we performed a screen of the collection of mutants carrying random autosomal insertions of PdL transposon. We isolated five candidate genes, of which two genes, Dek and Hel89B, encode proteins related to the formation of the epigenetic code, implying that epigenetic factors regulating gene expression may be involved in social experience-dependent modification of locomotor behavior.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detecting Brown Planthopper, Nilaparvata lugens (Stål) Damage in Rice Using Hyperspectral Remote Sensing 水稻褐飞虱危害的高光谱遥感检测

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-18 DOI: 10.1002/arch.70024

Eere Vidya Madhuri, Rabi N. Sahoo, Selvaprakash Ramalingam, Subhash Chander, Sharan Paramimuthu, Rajna Salim

{"title":"Detecting Brown Planthopper, Nilaparvata lugens (Stål) Damage in Rice Using Hyperspectral Remote Sensing","authors":"Eere Vidya Madhuri, Rabi N. Sahoo, Selvaprakash Ramalingam, Subhash Chander, Sharan Paramimuthu, Rajna Salim","doi":"10.1002/arch.70024","DOIUrl":"https://doi.org/10.1002/arch.70024","url":null,"abstract":"<div>\u0000 \u0000 Remote sensing is being increasingly used in agricultural stress management, offering real-time analysis of crop stress beyond what can be observed visually alone. Hopper burn, induced by brown planthopper (BPH, Nilaparvata lugens) is a significant challenge to rice production in India. This study aims to characterize the spectral reflectance of rice plants with different infestation levels of 2nd instar BPH nymphs across three varieties namely Pusa Basamti-1509, Pusa Basmati-1121, and TN-1 (susceptible variety to BPH). The differential infestation included 0, 5, 10, 20, 40, 80, 100, 200 number of nymphs. The spectral signatures of the crop were collected at 20 and 40 days after infestation (DAI). The results indicated a distinct change in reflectance pattern between healthy and infested rice plants across all three varieties in green (490–559 nm), yellow (560–584 nm), orange (585–639 nm), and red (640–699 nm) region of visible portion as affected by chlorophyll pigments and in NIR region (700–1800 nm) as affected by cell structure and in water absorption (1915 nm) region of SWIR portion. The specific wavelength band (470, 660,750, 1800, 1915 nm) showing a correlation above 0.8 with pest severity level, was identified as sensitive for assessing BPH damage. Analysis of reflectance changes across wavelengths highlighted that the first derivative has a strong correlation between BPH severity and reflectance in the green region (500–540 nm) and red edge position (680–760 nm). The amplitude of the red edge value decreased with an increase in the severity level of insects. This study identifies the sensitivity of visible and near-infrared regions in detecting and assessing BPH infestation severity.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microcystin-Lr-Induced Changes in Growth Performance, Intestinal Microbiota, and Lipid Metabolism of Black Soldier Fly Larvae (Hermetia illucens) 微囊藻毒素对黑兵蝇幼虫生长性能、肠道菌群和脂质代谢的影响

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-13 DOI: 10.1002/arch.70036

Peng Gu, Yi Ding, Wanqing Zhang, Kunlun Yang, Zengshuai Zhang, Xueli Ren, Hua Su, Hengfeng Miao

{"title":"Microcystin-Lr-Induced Changes in Growth Performance, Intestinal Microbiota, and Lipid Metabolism of Black Soldier Fly Larvae (Hermetia illucens)","authors":"Peng Gu, Yi Ding, Wanqing Zhang, Kunlun Yang, Zengshuai Zhang, Xueli Ren, Hua Su, Hengfeng Miao","doi":"10.1002/arch.70036","DOIUrl":"https://doi.org/10.1002/arch.70036","url":null,"abstract":"<div>\u0000 \u0000 Biological treatment by black soldier fly larvae (BSFL) has proven to be an effective method for the resource utilization of cyanobacteria, but the effects of microcystin-LR (MC-LR) in cyanobacteria on BSFL growth have not been adequately explored. To evaluate the inhibitory effect and toxic mechanism of MC-LR on BSFL, the growth performance and intestinal microbiota were examined after exposure to 0, 10, 100, and 1000 μg/kg of MC-LR. The larval weight and survival rate were each significantly inhibited by 21.53% and 21.49% compared with the control group, respectively, after exposure at a concentration of 1000 μg/kg MC-LR for 16 days. Lipid accumulation, intestinal inflammation, and oxidative stress were observed in three treatment groups, with dose-dependent inflammation ocurring in the intestine. Compared with the control group, superoxide dismutase and catalase activity levels were significantly increased by 74.91% and 49.58%, respectively, which confirmed the occurrence of oxidative stress induced by MC-LR. Furthermore, MC-LR altered the diversity of intestinal microbiota and increased the relative abundance of pathogenic bacteria (e.g., Paenibacillus, Clostridium_sensu_stricto_1, and Lachnoclostridium), which increased the risk of disease in BSFL and contributed to observed metabolic disorders. On the other hand, qRT-PCR analysis further confirmed the occurrence of oxidative stress and the activation of the peroxisome proliferator-activated receptor signaling pathway, resulting in the upregulation of fatty acid synthesis-related genes, ultimately leading to lipid accumulation and apoptosis. These findings provide valuable insights into the ecological risks associated with MC-LR during the process of cyanobacterial resource utilization.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroantennogram and Behavioral Responses of Nematus Hequensis Xiao Adult to Volatiles of Salix alba Leaves 河曲小线虫成虫对白柳叶挥发物的触角电位及行为反应

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-13 DOI: 10.1002/arch.70035

Zhenhao Song, Yiqu Chen, Xiao Dong, Zhaoxu Sun, Xinnan Guo, Kun Yu, Xiaoqin Tang, Jiancheng Zang

{"title":"Electroantennogram and Behavioral Responses of Nematus Hequensis Xiao Adult to Volatiles of Salix alba Leaves","authors":"Zhenhao Song, Yiqu Chen, Xiao Dong, Zhaoxu Sun, Xinnan Guo, Kun Yu, Xiaoqin Tang, Jiancheng Zang","doi":"10.1002/arch.70035","DOIUrl":"https://doi.org/10.1002/arch.70035","url":null,"abstract":"<div>\u0000 \u0000 The aim of this study was to investigate how leaf volatiles from Salix alba affect the orientation of Nematus hequensis Xiao adults towards host plants and contribute to the development of plant-based attractants for N. hequensis. Solid-phase microextraction (SPME) coupled with gas chromatograph-mass spectrometry (GC-MS) was used to extract, identify, and screen leaf volatiles from S. alba aged 10–15 years during the peak eclosion of N. hequensis. Electroantennogram (EAG) and Y-shaped olfactometry assays were utilized to identify volatiles with relative content > 1% and determine the optimal concentration for attracting, 1-day-old unmated males and females. The results showed that among the 15 volatiles with a relative content of more than 1%, (E)-2-hexenal, benzaldehyde, and 2-hydroxybenzaldehyde elicited the strongest EAG response in adult males and females at a concentration of 100 μg·μL-1, with (E)-2-hexenal exhibiting a greater effect than 2-hydroxybenzaldehyde, which in turn was more effective than benzaldehyde. At this concentration, the attractive effects of (E)-2-Hexenal, and benzaldehyde on adult males and females were significant. These findings provide an important basis for developing attractants and repellents used in control of this insect pest.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of Acetylation-Defective Heat Shock Protein 60 Inhibits the Proliferation of Nucleopolyhedrovirus in Bombyx mori 过表达乙酰化缺陷热休克蛋白60抑制家蚕核多角体病毒的增殖

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2025-02-13 DOI: 10.1002/arch.70038

Yao Li, Jiannan Wu, Yi You, Meng Miao, Wei Yu

{"title":"Overexpression of Acetylation-Defective Heat Shock Protein 60 Inhibits the Proliferation of Nucleopolyhedrovirus in Bombyx mori","authors":"Yao Li, Jiannan Wu, Yi You, Meng Miao, Wei Yu","doi":"10.1002/arch.70038","DOIUrl":"https://doi.org/10.1002/arch.70038","url":null,"abstract":"<div>\u0000 \u0000 Heat shock protein 60 (Hsp60), abundantly presents in mitochondria, is a highly conserved chaperone that maintains the stability and functionality of mitochondrial proteins, while also participating in the regulation of various cellular processes. As a member of the heat shock family, Hsp60 significantly influences viral proliferation. However, limited research is available on its role in the proliferation of entomopathogenic baculoviruses, particularly Bombyx mori nucleopolyhedrovirus (BmNPV). Our previous proteomics results showed a significant decrease of Hsp60 acetylation levels after BmNPV infection. To investigate the impact of Hsp60 deacetylation on viral proliferation, site-direct mutagenesis was performed to generate a deacetylated (K/R) mimic of Hsp60. We found that the acetylation level of lysine 362 (K362) decreased after BmNPV challenge. Furthermore, overexpression of deacetylation-mimicking Hsp60 reduced the chaperone activity of Hsp60, leading to impaired mitochondrial function, including increased reactive oxygen species (ROS) levels, decreased mitochondrial membrane potential, and reduced substrate protein Manganese-containing superoxide dismutase (Mn-SOD) activities, ultimately leading to inhibition of viral proliferation. This study establishes lysine 362 acetylation of Hsp60 as a model for Posttranslational modifications induced by host-virus interactions, providing new insights into potential antiviral strategies.</div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"118 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0