Structural Diversity of Adipokinetic Hormones in the Coleopteran Suborder Polyphaga (Excluding Cucujiformia)

IF 1.5 4区 农林科学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Gerd Gäde, Simone König, Heather G. Marco
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引用次数: 0

Abstract

Beetles are the largest animal group, in general. Phylogenetically, beetles belong to the order Coleoptera, the most species-rich of the Insecta. Coleoptera is divided into four suborders: Polyphaga, Adephaga, Archostemata, and Myxophaga. Specimens from the latter two are difficult to obtain, hence, we have focused our research into the adipokinetic hormone (AKH) peptide family on the former two suborders. Data on the Adephaga were concluded in 2017. The “core Polyphaga” consists of three series: Elateriformia, Staphyliniformia, and Cucujiformia; the latter was concluded in 2019. Here, we report on the AKH sequence(s) of 23 species of beetles from 4 families of Elateriformia, namely, the Buprestidae, Cantharidae, Elateridae, and Lampyridae; and 4 families of Staphyliniformia, namely, the Hydrophilidae, Silphidae, Lucanidae, and Scarabaeidae. Sequence elucidation by mass spectrometry or Edman degradation revealed 13 octapeptides: 5 are novel, 12 are beetle-specific and Schgr-AKH-II is produced in the basal Polyphaga (Elateriformia, in Hydrophilididae and Silphidae). Since Schgr-AKH-II is also found in Adephaga, this confirms the ancestral AKH of Coleoptera. The first change in sequence is recorded in Staphylinoidea with two different residues, notably, the switch from Phe4 to Tyr4. Duplication of AKH peptides is first seen in Lucanidae, as well as the appearance of atypical AKH sequences, such as Phe2, Met4, Leu4, or Phe7 encountered in the Scarabaeoidea. The vast majority of the pest beetles do not have beetle-specific AKHs or share the same AKH as nonharmful beetles. Ideas for finding a lead compound for green insecticides are discussed.

Abstract Image

鞘翅目多角亚目(不包括葫芦形目)脂肪动力激素的结构多样性。
一般来说,甲虫是最大的动物群体。从系统发育上讲,甲虫属于鞘翅目,是昆虫纲中种类最丰富的。鞘翅目分为四个亚目:多食亚目、无食亚目、无翅亚目和粘食亚目。后两者的标本很难获得,因此,我们将研究重点放在前两个亚目的脂肪动力学激素(AKH)肽家族上。Adephaga的数据于2017年完成。“核心多食虫”包括三个系列:Elateriformia、Staphyliniformia和Cucujiformia;后者于2019年结束。本文报道了鞘翅目4科23种甲虫的AKH序列(s),分别为:布氏科、斑蛾科、鞘翅科和鞘翅科;以及葡萄球菌属4科,即亲水蝇科、银蝇科、露蝇科和金龟科。通过质谱或Edman降解对13个八肽进行了序列解析,其中5个是新的,12个是甲虫特异性的,Schgr-AKH-II是在基础多食动物(Elateriformia, in hydrophildidae and Silphidae)中产生的。由于在Adephaga也发现了Schgr-AKH-II,这证实了鞘翅目祖先的AKH。第一个序列变化记录在Staphylinoidea中,有两个不同的残基,值得注意的是,从Phe4切换到Tyr4。首先在Lucanidae中发现了AKH肽的重复,以及在Scarabaeoidea中遇到的非典型AKH序列的出现,例如Phe2, Met4, Leu4或Phe7。绝大多数害虫甲虫没有甲虫特有的AKH,或者与无害甲虫共享相同的AKH。讨论了寻找绿色杀虫剂先导化合物的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
115
审稿时长
12 months
期刊介绍: Archives of Insect Biochemistry and Physiology is an international journal that publishes articles in English that are of interest to insect biochemists and physiologists. Generally these articles will be in, or related to, one of the following subject areas: Behavior, Bioinformatics, Carbohydrates, Cell Line Development, Cell Signalling, Development, Drug Discovery, Endocrinology, Enzymes, Lipids, Molecular Biology, Neurobiology, Nucleic Acids, Nutrition, Peptides, Pharmacology, Pollinators, Proteins, Toxicology. Archives will publish only original articles. Articles that are confirmatory in nature or deal with analytical methods previously described will not be accepted.
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