Archives of Insect Biochemistry and Physiology最新文献

{"title":"Molecular characterization and functional analysis of the ecdysone receptor isoform (EcR) from the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)","authors":"Zhishan Cao,&nbsp;Olha Bakumenko,&nbsp;Volodymyr Vlasenko,&nbsp;Weihai Li,&nbsp;Jinjun Cao","doi":"10.1002/arch.22110","DOIUrl":"https://doi.org/10.1002/arch.22110","url":null,"abstract":"<p>20-Hydroxyecdysone (20E) plays a vital role in a series of biological processes, via the nuclear receptors, EcR/USP by activating the ecdysone regulatory cascade. To clarify the role of EcR during the development of <i>Grapholita molesta</i>, the complementary DNA of ecdysone receptor isoform B1 (<i>GmEcR-B1</i>) was obtained from the transcriptome of <i>G. molesta</i> and verified by PCR. Alignment analysis revealed that the deduced protein sequence of GmEcR-B1 was highly homologous to EcR proteins identified in other lepidopteran species, especially the EcR-B1 isoform in <i>Spodoptera litura</i>. Quantitative real-time PCR showed that <i>GmEcRs</i> was expressed at all test developmental stages, and the expression level of <i>GmEcRs</i> was relatively higher during the period of the 3rd day of fifth instar larvae to 2nd of pupa than those in other stages. Moreover, the messenger RNA of <i>GmEcRs</i> was much more strongly expressed in the Malpighian tubule and epidermis than those in other tissues, which suggests that this gene may function in a tissue-specific manner during larval development. Silencing of <i>GmEcRs</i> could significantly downregulate the transcriptional level of ecdysone-inducible genes and result in increased mortality during metamorphosis and prolonged prepupal duration. Taken together, the present results indicate that <i>GmEcRs</i> may directly or indirectly affect the development of <i>G. molesta</i>.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140546644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local age-dependent neuromodulation in Rhodnius prolixus antennae","authors":"Marcelo Gustavo Lorenzo,&nbsp;Gabriel da Rocha Fernandes,&nbsp;Jose Manuel Latorre-Estivalis","doi":"10.1002/arch.22106","DOIUrl":"https://doi.org/10.1002/arch.22106","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Kissing bugs do not respond to host cues when recently molted and only exhibit robust host-seeking several days after ecdysis. Behavioral plasticity has peripheral correlates in antennal gene expression changes through the week after ecdysis. The mechanisms regulating these peripheral changes are still unknown, but neuropeptide, G-protein coupled receptor, nuclear receptor, and <i>takeout</i> genes likely modulate peripheral sensory physiology. We evaluated their expression in antennal transcriptomes along the first week postecdysis of <i>Rhodnius prolixus</i> 5th instar larvae. Besides, we performed clustering and co-expression analyses to reveal relationships between neuromodulatory (NM) and sensory genes. Significant changes in transcript abundance were detected for 50 NM genes. We identified 73 sensory-related and NM genes that were assigned to nine clusters. According to their expression patterns, clusters were classified into four groups: two including genes up or downregulated immediately after ecdysis; and two with genes with expression altered at day 2. Several NM genes together with sensory genes belong to the first group, suggesting functional interactions. Co-expression network analysis revealed a set of genes that seem to connect with sensory system maturation. Significant expression changes in NM components were described in the antennae of <i>R. prolixus</i> after ecdysis, suggesting that a local NM system acts on antennal physiology. These changes may modify the sensitivity of kissing bugs to host cues during this maturation interval.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140541174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and expression patterns of somatic piRNAs and PIWI genes in Riptortus pedestris (Hemiptera: Alydidae)","authors":"Yipeng Ren,&nbsp;Wenhao Dong,&nbsp;Wenjun Bu,&nbsp;Huaijun Xue","doi":"10.1002/arch.22107","DOIUrl":"https://doi.org/10.1002/arch.22107","url":null,"abstract":"<p>RNA interference (RNAi)-based gene silencing is a feasible and sustainable technology for the management of hemipteran pests by double-stranded RNA involvement, including small-interfering RNA, microRNA, and Piwi-interacting RNA (piRNA) pathways, that may help to decrease the usage of chemical insecticides. However, only a few data are available on the somatic piRNAs and their biogenesis genes in <i>Riptortus pedestris</i>, which serves as a significant pest of soybean (<i>Glycine max</i>). In this study, two family members of the <i>PIWI</i> gene were identified and characterized in <i>R. pedestris</i>, containing <i>Argonaute3</i> (Rp<i>Ago3</i>) and <i>Aubergine</i> (Rp<i>Aub</i>) genes with conserved protein domains, and their clusters were validated by phylogenetic analysis. In addition, they were widely expressed in all developmental stages of the whole body of <i>R. pedestris</i> and had lower expression levels in <i>R. pedestris</i> guts under different rearing conditions based on previous transcriptome sequencing. Furthermore, abundant clean reads were filtered to a total number of 45,998 piRNAs with uridine bias at the first nucleotide (nt) position and 26–32 nt in length by mapping onto the reference genome of <i>R. pedestris</i> according to our previous whole-transcriptome sequencing. Finally, our data revealed that gut bacterial changes were significantly positively or negatively associated with differentially expressed piRNAs among the five comparison groups with Pearson correlation analysis. In conclusion, these findings paved new avenues for the application of RNAi-based biopesticides for broad-spectrum hemipteran pest control.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Six complete mitochondrial genomes of ground beetles from the Harpalinae and Carabinae (Coleoptera, Carabidae) with phylogenetic analysis based on mitogenomic data","authors":"Xingyu Lin,&nbsp;Nan Song,&nbsp;Miaomiao Wang","doi":"10.1002/arch.22108","DOIUrl":"https://doi.org/10.1002/arch.22108","url":null,"abstract":"<p>In this study, we employed high-throughput sequencing technology to determine the complete mitochondrial genomes of six ground beetles, encompassing five Harpalinae species and one Carabinae species. The sizes of mitochondrial genomes ranged from 15,334 to 16,972 bp, encompassing 37 genes, including 13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes. Furthermore, each species was found to possess a putative control region. Combining with 65 published mitochondrial genome sequences of Carabidae as ingroups and four species from Trachypachidae, Gyrinidae and Dytiscidae as outgroups, we conducted phylogenetic analyses utilizing Maximum likelihood and Bayesian inference methods. Moreover, we reconstructed a species tree of Carabidae based on mitochondrial genome data using the coalescent-based species tree method (ASTRAL). The results revealed that the family Carabidae was not a monophyletic group. The subfamily Harpalinae was supported to be a monophyletic group in Maximum likelihood analysis. Although the subfamily Carabinae was found to be nonmonophyletic in the concatenation analyses under both Maximum likelihood and Bayesian inference criteria, it was identified as a monophyletic group in the species tree analysis.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140345614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “The extraordinary rearrangement of mitochondrial genome of the wheat pest, Aptinothrips stylifer and the mitochondrial phylogeny of Thripidae (Thysanoptera)”","authors":"","doi":"10.1002/arch.22095","DOIUrl":"10.1002/arch.22095","url":null,"abstract":"<p>Li, C., Gao, Y., Wang, D. &amp; Dang, L. (2024) The extraordinary rearrangement of mitochondrial genome of the wheat pest, <i>Aptinothrips stylifer</i> and the mitochondrial phylogeny of Thripidae (Thysanoptera). <i>Archives of Insect Biochemistry and Physiology</i>, 115(1), e22086. Available from: https://doi.org/10.1002/arch.22086.</p><p>In Figure 1, the thrips picture in the circular representation of the complete mt genome of <i>Aptinothrips stylifer</i> was incorrect. The correct figure is shown below.</p><p>We apologize for this error.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140302255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity, adaptation, and evolution II: Preface","authors":"Rui-E Nie,&nbsp;Shou-Ke Zhang,&nbsp;Huai-Jun Xue","doi":"10.1002/arch.22097","DOIUrl":"10.1002/arch.22097","url":null,"abstract":"&lt;p&gt;This special issue focuses on the evolution of insects. The following is a general introduction to the articles in this special issue. It includes 14 research articles that provide new insights into insect diversity and evolution.&lt;/p&gt;&lt;p&gt;The first topic covered in this special issue is the comparative analysis of insect mitogenomes and phylogenetics. Seven papers were published on this subject, including those by Pakrashi et al. (&lt;span&gt;2023&lt;/span&gt;), Qi et al. (&lt;span&gt;2023&lt;/span&gt;), Huang et al. (&lt;span&gt;2023&lt;/span&gt;), Li et al. (&lt;span&gt;2023&lt;/span&gt;), Guo et al. (&lt;span&gt;2023&lt;/span&gt;), Li et al. (&lt;span&gt;2024b&lt;/span&gt;) and Lin et al. (&lt;span&gt;2024&lt;/span&gt;). Pakrashi et al. (&lt;span&gt;2023&lt;/span&gt;) specifically focused on the invasive pest species &lt;i&gt;Thrips parvispinus&lt;/i&gt;, characterizing its complete mitochondrial genome. The phylogenetic analysis supported the monophyly of Phlaeothripinae and Idolothripinae under suborder Tubulifera. Qi et al. (&lt;span&gt;2023&lt;/span&gt;) analyzed the mitogenomes of five &lt;i&gt;Thienemanniella&lt;/i&gt; species and explored their interspecific relationships based on mitogenomics data. Huang et al. (&lt;span&gt;2023&lt;/span&gt;) reported four mitogenomes of Elateridae and conducted comparative analyses to further understand their evolutionary patterns. The phylogenetic results confirmed the monophyly of Cardiophorinae, Agrypninae, and Elaterinae with high support. Li et al. (&lt;span&gt;2024b&lt;/span&gt;) obtained the mitogenome of &lt;i&gt;Cricotopu&lt;/i&gt; (&lt;i&gt;Pseudocricotopus&lt;/i&gt;) &lt;i&gt;cf. montanus&lt;/i&gt; and nine other &lt;i&gt;Cricotopus&lt;/i&gt; species for phylogenetic analysis. Guo et al. (&lt;span&gt;2023&lt;/span&gt;) expored the interrelationships among the syrphid and pipunculid members of Syrphoidea using mitochondial genome sequences of 152 taxa (9 newly sequenced). The results approved that the Syrphoidea was nonmonophyletic and Microdontinae was sister to all other Syrphidae. Li et al. (&lt;span&gt;2024a&lt;/span&gt;) demonstrates the close relationship between gene rearrangements and phylogeny in Thripidae. The phylogenetic trees confirmed the monophyly of four families of Thysanoptera and the phylogenetic status of &lt;i&gt;Aptinothrips stylifer&lt;/i&gt;. Lin et al. (&lt;span&gt;2024&lt;/span&gt;) combined six newly seqenced and 65 published mitochondrial genomes from Carabidae to reconstruct the phylogenetic tree of this family, they suggested that Carabidae is not a monophyletic group, but the monophyly of each subfamilies (Carabinae and Harpalinae) is still controversial.&lt;/p&gt;&lt;p&gt;The second topic addressed in this special issue is the integrative taxonomy of insects, as discussed by Yu et al. (&lt;span&gt;2023&lt;/span&gt;) and Li et al. (&lt;span&gt;2023&lt;/span&gt;). Yu et al. (&lt;span&gt;2023&lt;/span&gt;) utilized a combination of cladistics, ABGD, jMOTU, bPTP, and morphologic methods to confirm the existence of nine &lt;i&gt;Ceriagrion&lt;/i&gt; species in China. Their study also reported two synonyms, one new record, one distribution elimination, and three misidentifications. Li et al. (&lt;span&gt;2023&lt;/span&gt;) reviewed &lt;i&gt;Rheotanytarsus guineensis&lt;/i&gt; species group from","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140292617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of an entomopathogenic fungal virulence against the seedcorn maggot, Delia platura, by suppressing immune responses with a bacterial culture broth of Photorhabdus temperata subsp. temperata","authors":"Eticha Abdisa,&nbsp;Hyunje Park,&nbsp;Jiyoon Kwon,&nbsp;Gahyeon Jin,&nbsp;Mojtaba Esmaeily,&nbsp;Yonggyun Kim","doi":"10.1002/arch.22103","DOIUrl":"10.1002/arch.22103","url":null,"abstract":"<p>In Korea, there are two maggot species in the <i>Delia</i> genus that commonly infest the roots and stems of the Welsh onion, thus causing serious economic damage on the crop at the seedling stage. In this study, the seedcorn maggot (<i>Delia platura</i>) was detected in onion fields in two different localities in Korea. After overwintering, maggot infestations occurred throughout the entire growing seasons from transplantation to harvest, but their specific patterns of occurrence varied in the two localities examined. Entomopathogenic fungi induced significant virulence against the maggot larvae, in which a strain of <i>Beauveria bassiana</i> was effective, though it exhibited limited mortality in its insecticidal activity. To enhance this insecticidal activity, a culture broth from an entomopathogenic bacterium, <i>Photorhabdus temperata temperata</i> (Ptt), was added to <i>B. bassiana</i> treatment. The addition of Ptt broth significantly increased the insecticidal activity of <i>B. bassiana</i> in a dose-dependent manner. To elucidate this enhancement in insecticidal activity, the immunosuppressive activity of Ptt broth was assessed by identifying the immune responses of the seedcorn maggots. The seedcorn maggots possessed at least three different hemocytes with plasmatocytes, crystal cells, and lamellocytes. These hemocytes exhibited nodule formation in response to the fungal infection. In addition to the cellular immunity, the maggots exhibited inducible expressions of antimicrobial peptide (AMP) genes such as <i>cecropin</i> and <i>defensin</i>. The addition of Ptt broth suppressed the nodule formation and the AMP expressions in response to the fungal infection. Altogether, this study demonstrated the innate immune responses in a non-model insect, <i>D. platura</i>, along with the application of immunosuppression to develop a highly efficient biological control by enhancing the virulence of <i>B. bassiana</i>.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1H-NMR revealed pyruvate as a differentially abundant metabolite in the venom glands of Apis cerana and Apis mellifera","authors":"Xing Zheng,&nbsp;Yanjun Liu,&nbsp;Rongshen Wang,&nbsp;Mingyang Geng,&nbsp;Jinliang Liu,&nbsp;Zhenxing Liu,&nbsp;Yazhou Zhao","doi":"10.1002/arch.22104","DOIUrl":"https://doi.org/10.1002/arch.22104","url":null,"abstract":"<p>As a common defense mechanism in Hymenoptera, bee venom has complex components. Systematic and comprehensive analysis of bee venom components can aid in early evaluation, accurate diagnosis, and protection of organ function in humans in cases of bee stings. To determine the differences in bee venom composition and metabolic pathways between <i>Apis cerana</i> and <i>Apis mellifera</i>, proton nuclear magnetic resonance (¹H-NMR) technology was used to detect the metabolites in venom samples. A total of 74 metabolites were identified and structurally analyzed in the venom of <i>A. cerana</i> and <i>A. mellifera</i>. Differences in the composition and abundance of major components of bee venom from <i>A. cerana</i> and <i>A. mellifera</i> were mapped to four main metabolic pathways: valine, leucine and isoleucine biosynthesis; glycine, serine and threonine metabolism; alanine, aspartate and glutamate metabolism; and the tricarboxylic acid cycle. These findings indicated that the synthesis and metabolic activities of proteins or polypeptides in bee venom glands were different between <i>A. cerana</i> and <i>A. mellifera</i>. Pyruvate was highly activated in 3 selected metabolic pathways in <i>A. mellifera</i>, being much more dominant in <i>A. mellifera</i> venom than in <i>A. cerana</i> venom. These findings indicated that pyruvate in bee venom glands is involved in various life activities, such as biosynthesis and energy metabolism, by acting as a precursor substance or intermediate product.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140164424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of cuticle protein superfamily in Frankliniella occidentalis provide insight into the control of both insect vectors and plant virus","authors":"Yang Zheng,&nbsp;Yinghao Feng,&nbsp;Zhejin Li,&nbsp;Junwen Wang","doi":"10.1002/arch.22102","DOIUrl":"10.1002/arch.22102","url":null,"abstract":"<p>The structural cuticle proteins (CPs) play important roles in the development and fitness of insects. However, knowledge about CP gene superfamily is limited in virus-transmitting insect vectors, although its importance on transmission of plant virus has been gradually emphasized. In this study, the genome-wide identification of CP superfamily was conducted in western flower thrips <i>Frankliniella occidentalis</i> that is the globally invasive pest and plant virus vector pest. The pest transmits notorious tomato spotted wilt virus (TSWV) around the world, causing large damage to a wide array of plants. One hundred and twenty-eight <i>F. occidentalis</i> CP genes (<i>FoCP</i>s) were annotated in this study and they were classified into 10 distinct families, including 68 <i>CPR</i>s, 16 <i>CPAP1</i>s, 6 <i>CPAP3</i>s, 2 <i>CPCFC</i>s, 10 <i>Tweedle</i>s, 4 <i>CPF</i>s, 16 <i>CPLCP</i>s, and 6 <i>CPG</i>s. The comprehensive analysis was performed including phylogenetic relationship, gene location and gene expression profiles during different development stages of <i>F. occidentalis</i>. Transcriptome analysis revealed more than 30% <i>FoCP</i>s were upregulated at least 1.5-fold when <i>F. occidentalis</i> was infected by TSWV, indicating their potential involvement in TSWV interactions. Our study provided an overview of <i>F. occidentalis</i> CP superfamily. The study gave a better understand of CP's role in development and virus transmission, which provided clues for reducing viral damages through silencing CP genes in insect vectors.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140157445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the diversity of symbionts in fat body of long- and short-winged brown planthoppers","authors":"Linlin Fan,&nbsp;Guangxiang Guan,&nbsp;Jingjing Zhao,&nbsp;Danting Li,&nbsp;Xiaoping Yu,&nbsp;Xuping Shentu","doi":"10.1002/arch.22096","DOIUrl":"10.1002/arch.22096","url":null,"abstract":"<p>The microbial community structure plays an important role in the internal environment of brown planthopper (BPH), <i>Nilaparvata lugens</i> (Hemiptera: Delphacidae), which is an indispensable part to reflect the internal environment of BPH. Wing dimorphism is a strategy for balancing flight and reproduction of insects. Here, quantitative fluorescence PCR was used to analyse the number and changes of the symbionts in the fat body of long- and short-winged BPHs at different developmental stages. A metagenomic library was constructed based on the 16 S rRNA sequence and internal transcribed spacer sequence for high-throughput sequencing, to analyze the community structure and population number of the symbionts of long- and short-winged BPHs, and to make functional prediction. This study enriches the connotation of BPH symbionts, and laid a theoretical foundation for the subsequent study of BPH-symbionts interaction and the function of symbionts in the host.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140157465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}