Archives of Insect Biochemistry and Physiology最新文献_第10页

Composition and abundance of midgut plasma membrane proteins in two major hemipteran vectors of plant viruses, Bemisia tabaci and Myzus persicae 两种主要的植物病毒半翅目载体--Bemisia tabaci 和 Myzus persicae--的中肠质膜蛋白的组成和丰度。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-25 DOI: 10.1002/arch.22133

Jaime Jiménez, Ruchir Mishra, Xinyue Wang, Ciara M. Magee, Bryony C. Bonning

{"title":"Composition and abundance of midgut plasma membrane proteins in two major hemipteran vectors of plant viruses, Bemisia tabaci and Myzus persicae","authors":"Jaime Jiménez, Ruchir Mishra, Xinyue Wang, Ciara M. Magee, Bryony C. Bonning","doi":"10.1002/arch.22133","DOIUrl":"10.1002/arch.22133","url":null,"abstract":"Multiple species within the order Hemiptera cause severe agricultural losses on a global scale. Aphids and whiteflies are of particular importance due to their role as vectors for hundreds of plant viruses, many of which enter the insect via the gut. To facilitate the identification of novel targets for disruption of plant virus transmission, we compared the relative abundance and composition of the gut plasma membrane proteomes of adult Bemisia tabaci (Hemiptera: Aleyrodidae) and Myzus persicae (Hemiptera: Aphididae), representing the first study comparing the gut plasma membrane proteomes of two different insect species. Brush border membrane vesicles were prepared from dissected guts, and proteins extracted, identified and quantified from triplicate samples via timsTOF mass spectrometry. A total of 1699 B. tabaci and 1175 M. persicae proteins were identified. Following bioinformatics analysis and manual curation, 151 B. tabaci and 115 M. persicae proteins were predicted to localize to the plasma membrane of the gut microvilli. These proteins were further categorized based on molecular function and biological process according to Gene Ontology terms. The most abundant gut plasma membrane proteins were identified. The ten plasma membrane proteins that differed in abundance between the two insect species were associated with the terms “protein binding” and “viral processes.” In addition to providing insight into the gut physiology of hemipteran insects, these gut plasma membrane proteomes provide context for appropriate identification of plant virus receptors based on a combination of bioinformatic prediction and protein localization on the surface of the insect gut.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the contribution of the salivary gland and midgut to digestion in the swede midge (Contarinia nasturtii) through a genomics-guided approach 通过基因组学指导方法探索獐蝽唾液腺和中肠对消化的贡献。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-22 DOI: 10.1002/arch.22135

Boyd A. Mori, Cathy Coutu, Martin A. Erlandson, Dwayne D. Hegedus

{"title":"Exploring the contribution of the salivary gland and midgut to digestion in the swede midge (Contarinia nasturtii) through a genomics-guided approach","authors":"Boyd A. Mori, Cathy Coutu, Martin A. Erlandson, Dwayne D. Hegedus","doi":"10.1002/arch.22135","DOIUrl":"10.1002/arch.22135","url":null,"abstract":"The larvae of Contarinia nasturtii (Kieffer) (Diptera: Cecidomyiidae), the swede midge, targets the meristem of brassica crops where they induce the formation of galls and disrupt seed and vegetable production. Previously, we examined the salivary gland transcriptome of newly-hatched first instar larvae as they penetrated the host and initiated gall formation. Here we examine the salivary gland and midgut transcriptome of third instar larvae and provide evidence for cooperative nutrient acquisition beginning with secretion of enzymes and feeding facilitators followed by gastrointestinal digestion. Sucrose, presumably obtained from the phloem, appeared to be a major nutrient source as several α-glucosidases (sucrases, maltases) and β-fructofuranosidases (invertases) were identified. Genes encoding β-fructofuranosidases/invertases were among the most highly expressed in both tissues and represented two distinct gene families that may have originated via horizontal gene transfer from bacteria. The importance of the phloem as a nutrient source is underscored by the expression of genes encoding regucalcin and ARMET (arginine-rich mutated in early stages of tumor) which interfere with calcium signalling and prevent sieve tube occlusion. Lipids, proteins, and starch appear to serve as a secondary nutrient sources. Genes encoding enzymes involved in the detoxification of glucosinolates (myrosinases, arylsulfatases, and glutathione-S-transferases) were expressed indicative of Brassicaceae host specialization. The midgut expressed simple peritrophins and mucins typical of those found in Type II peritrophic matrices, the first such description for a gall midge.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silence of Aminopeptidase N 2 gene reveals the trade-offs for acquiring Cry1Ac resistance in Plutella xylostella 氨基肽酶 N 2 基因的沉默揭示了木虱获得 Cry1Ac 抗性的权衡。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-17 DOI: 10.1002/arch.22131

Zhuohong Lv, Shuwen Yu, Yafei Zhao, Zhongxia Yang

{"title":"Silence of Aminopeptidase N 2 gene reveals the trade-offs for acquiring Cry1Ac resistance in Plutella xylostella","authors":"Zhuohong Lv, Shuwen Yu, Yafei Zhao, Zhongxia Yang","doi":"10.1002/arch.22131","DOIUrl":"10.1002/arch.22131","url":null,"abstract":"Bacillus thuringiensis (Bt) is widely used as a biopesticide worldwide. To date, at least eight pest species have been found to be resistant to Bt in the field. As the first pest that was reported having resistance to Bt in the field, considerable research has been done on the mechanisms of Bt resistance in Plutella xylostella. However, whether the acquisition of Bt resistance by P. xylostella comes at a fitness cost is also a valuable question. In this study, Aminopeptidase-N 2 (APN2), a Cry toxin receptor gene of P. xylostella, was knocked down by RNA interference, resulting in improved resistance to Cry1Ac. It was also found that larval mortality of APN2 knockdown P. xylostella was significantly higher than that of the control, while the pupation rate, pupal weight, eclosion rate, fecundity (egg/female), hatchability, and female adult longevity were significantly lower in APN2 knockdown P. xylostella than in the control. These results illustrate that if Cry1Ac resistance was obtained only through the reduction of APN2 expression, P. xylostella would need to incur some fitness costs for it.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and characterization of the histone variant gene H2A.Z in Bombyx mori 粘虫组蛋白变异基因 H2A.Z 的克隆和特征描述。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-17 DOI: 10.1002/arch.22136

Jun Mei, Kunling Xu, Yuyi Huang, Jingwei Zhang, Qitao Qian, Jia Dong, Fudan Tong, Wei Yu, Meng Miao

{"title":"Cloning and characterization of the histone variant gene H2A.Z in Bombyx mori","authors":"Jun Mei, Kunling Xu, Yuyi Huang, Jingwei Zhang, Qitao Qian, Jia Dong, Fudan Tong, Wei Yu, Meng Miao","doi":"10.1002/arch.22136","DOIUrl":"10.1002/arch.22136","url":null,"abstract":"H2A.Z, the most evolutionarily conserved variant of histone H2A, plays a pivotal role in chromatin remodeling and contributes significantly to gene transcription and genome stability. However, the role of H2A.Z in the silkworm (Bombyx mori) remains unclear. In this study, we cloned the BmH2A.Z from B. mori. The open reading frame of BmH2A.Z is 390 bp, encoding 129 amino acids, with a confirmed molecular weight of 13.4 kDa through prokaryotic expression analysis. Sequence analysis revealed that BmH2A.Z has a conserved H2A.Z domain and is closely related to the systemic evolution of other known H2A.Zs. The expression profile of BmH2A.Z at various developmental stages of the B. mori exhibited the highest expression level in the 1st instar, followed by the grain stage and the 2nd instar, and the lowest expression level in the moth. The highest transcript level of BmH2A.Z was observed in the head, with relatively lower levels detected in the blood than in the other tissues under consideration. In addition, the upregulation of BmH2A.Z resulted in the amplified expression of B. mori nucleopolyhedrovirus (BmNPV) genes, thus facilitating the proliferation of BmNPV. This study establishes a foundation for investigating the role of BmH2A.Z in B. mori and its participation in virus-host interactions.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland 消耗脂质储存液滴-1可延缓果蝇唾液腺的内再复制进程并诱导细胞死亡。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-11 DOI: 10.1002/arch.22132

Yen D. H. Nguyen, Tuan L. A. Pham, Taisei Nishihara, Kaeko Kamei, Duy Binh Tran

{"title":"Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland","authors":"Yen D. H. Nguyen, Tuan L. A. Pham, Taisei Nishihara, Kaeko Kamei, Duy Binh Tran","doi":"10.1002/arch.22132","DOIUrl":"10.1002/arch.22132","url":null,"abstract":"Perilipins are evolutionarily conserved from insects to mammals. Drosophila lipid storage droplet-1 (LSD-1) is a lipid storage droplet membrane surface-binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD-1 during specific tissue development remains under investigation. This study demonstrated the role of LSD-1 in salivary gland development. Knockdown of Lsd-1 in the salivary gland was established using the GAL4/UAS system. The third-instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant Drosophila also showed the same phenotype. The depletion of LSD-1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. Lsd-1 genetically interacted with Myc in the third-instar larvae. These results demonstrate that LSD-1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD-1 in regulating salivary gland development and the interaction between LSD-1 and Myc.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bombyx mori UFL1 facilitates BmNPV proliferation by regulating host cell apoptosis through PERK Bombyx mori UFL1通过PERK调节宿主细胞凋亡，从而促进BmNPV增殖。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-08 DOI: 10.1002/arch.22127

Yijun Zheng, Haonan Meng, Xiaochun Jiang, Shoujun Huang

引用次数: 0

Insights from different reproductive gene knockdowns via RNA interference in the lady beetle Eriopis connexa: Establishing a new model for molecular studies on natural enemies 通过核糖核酸（RNA）干扰敲除瓢虫不同生殖基因的启示：建立天敌分子研究的新模型。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-07 DOI: 10.1002/arch.22125

Ianne Caroline da Silva Nobre, Roberta Ramos Coelho, Felipe Marinho Coutinho de Souza, Manoely Abreu Reis, Jorge Braz Torres, José Dijair Antonino

{"title":"Insights from different reproductive gene knockdowns via RNA interference in the lady beetle Eriopis connexa: Establishing a new model for molecular studies on natural enemies","authors":"Ianne Caroline da Silva Nobre, Roberta Ramos Coelho, Felipe Marinho Coutinho de Souza, Manoely Abreu Reis, Jorge Braz Torres, José Dijair Antonino","doi":"10.1002/arch.22125","DOIUrl":"10.1002/arch.22125","url":null,"abstract":"Insect pest control can be achieved by the application of RNA interference (RNAi), a key molecular tool in functional genomics. Whereas most RNAi research has focused on insect pests, few studies have been performed on natural enemies. Validating the efficacy of RNAi in natural enemies is crucial for assessing its safety and enabling molecular research on these organisms. Here, we assessed the efficacy of RNAi in the ladybird beetle Eriopis connexa Germar (Coleoptera: Coccinellidae), focusing on genes related to reproduction, such as vitellogenin (Vg) and its receptor (VgR). In the transcriptome of E. connexa, we found one VgR (EcVgR) and two Vg genes (EcVg1 and EcVg2). These genes have been validated by in silico analyses of functional domains and evolutionary relationships. Five-day-old females were injected with 500 ng/µL of a specific double-stranded RNA (dsRNA) (dsEcVg1, dsEcVg2, or dsEcVgR) for RNAi tests, while nonspecific dsRNA (dsGFP or dsAgCE8.1) was used as a control. Interestingly, dsEcVg2 was able to knockdown both Vg genes, while dsEcVg1 could silence only EcVg1. Additionally, the viability of the eggs was significantly reduced when both Vg genes were knocked down at the same time (after treatment with dsEcVg2 or “dsEcVg1+dsEcVg2”). Ultimately, malformed, nonviable eggs were produced when EcVgR was silenced. Interestingly, no dsRNA treatment had an impact on the quantity of eggs laid. Therefore, the feasibility of RNAi in E. connexa has been confirmed, suggesting that this coccinellid is an excellent Neotropical model for molecular research on natural enemies and for studying RNAi nontarget effects.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving bee feed recipes to safeguard honeybee colonies during times of food scarcity 改进蜜蜂饲料配方，在食物匮乏时期保护蜜蜂群落。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-07 DOI: 10.1002/arch.22129

Xue Wang, Xing Zheng, Nana Guo, Mingyang Geng, Rongshen Wang, Ting Huang, Quanzhi Ji, Zhenxing Liu, Yazhou Zhao

{"title":"Improving bee feed recipes to safeguard honeybee colonies during times of food scarcity","authors":"Xue Wang, Xing Zheng, Nana Guo, Mingyang Geng, Rongshen Wang, Ting Huang, Quanzhi Ji, Zhenxing Liu, Yazhou Zhao","doi":"10.1002/arch.22129","DOIUrl":"10.1002/arch.22129","url":null,"abstract":"In beekeeping, when natural nectar or pollen sources become limited, it is crucial to provide supplemental bee feed to maintain the viability of the bee colony. This study was conducted during the autumn food shortage season, during which bees were fed with different proportions of modified bee feed. We identified an optimal bee diet by evaluating honeybee longevity, food consumption, body weight, and gut microbe distribution, with natural pollen serving as a control diet. The results indicated that bees preferred a mixture of 65% defatted soy flour, 20% corn protein powder, 13% wheat germ flour, 2% yeast powder, and a 50% sucrose solution. This bee food recipe significantly increased the longevity, feed consumption, and body weight of bees. The group fed the natural pollen diet exhibited a greater abundance of essential intestinal bacteria. The bee diets used in this study contained higher protein levels and lower concentrations of unsaturated fatty acids and vitamins than did the diets stored within the colonies. Therefore, we propose that incorporating both bee feed and natural pollen in beekeeping practices will achieve more balanced nutritional intake.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap-sucking insects 棉蚜唾液腺转录组学及其与其他吸食汁液昆虫的比较分析

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-06-11 DOI: 10.1002/arch.22123

Shanmugasundram Pavithran, Marimuthu Murugan, Jayakanthan Mannu, Chakkarai Sathyaseelan, Venkatasamy Balasubramani, Sankarasubramanian Harish, Senthil Natesan

{"title":"Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap-sucking insects","authors":"Shanmugasundram Pavithran, Marimuthu Murugan, Jayakanthan Mannu, Chakkarai Sathyaseelan, Venkatasamy Balasubramani, Sankarasubramanian Harish, Senthil Natesan","doi":"10.1002/arch.22123","DOIUrl":"10.1002/arch.22123","url":null,"abstract":"Aphids are sap-sucking insects responsible for crop losses and a severe threat to crop production. Proteins in the aphid saliva are integral in establishing an interaction between aphids and plants and are responsible for host plant adaptation. The cotton aphid, Aphis gossypii (Hemiptera: Aphididae) is a major pest of Gossypium hirsutum. Despite extensive studies of the salivary proteins of various aphid species, the components of A. gossypii salivary glands are unknown. In this study, we identified 123,008 transcripts from the salivary gland of A. gossypii. Among those, 2933 proteins have signal peptides with no transmembrane domain known to be secreted from the cell upon feeding. The transcriptome includes proteins with more comprehensive functions such as digestion, detoxification, regulating host defenses, regulation of salivary glands, and a large set of uncharacterized proteins. Comparative analysis of salivary proteins of different aphids and other insects with A. gossypii revealed that 183 and 88 orthologous clusters were common in the Aphididae and non-Aphididae groups, respectively. The structure prediction for highly expressed salivary proteins indicated that most possess an intrinsically disordered region. These results provide valuable reference data for exploring novel functions of salivary proteins in A. gossypii with their host interactions. The identified proteins may help develop a sustainable way to manage aphid pests.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and application of carrier-free and carrier-based nanopesticides in pest management 无载体和基于载体的纳米杀虫剂的制造和在害虫防治中的应用。

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-06-11 DOI: 10.1002/arch.22124

Wenhao Dong, Yipeng Ren, Huaijun Xue

引用次数: 0