Archives of Insect Biochemistry and Physiology最新文献_第10页

Insights from different reproductive gene knockdowns via RNA interference in the lady beetle Eriopis connexa: Establishing a new model for molecular studies on natural enemies 通过核糖核酸（RNA）干扰敲除瓢虫不同生殖基因的启示：建立天敌分子研究的新模型。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-07 DOI: 10.1002/arch.22125

Ianne Caroline da Silva Nobre, Roberta Ramos Coelho, Felipe Marinho Coutinho de Souza, Manoely Abreu Reis, Jorge Braz Torres, José Dijair Antonino

{"title":"Insights from different reproductive gene knockdowns via RNA interference in the lady beetle Eriopis connexa: Establishing a new model for molecular studies on natural enemies","authors":"Ianne Caroline da Silva Nobre, Roberta Ramos Coelho, Felipe Marinho Coutinho de Souza, Manoely Abreu Reis, Jorge Braz Torres, José Dijair Antonino","doi":"10.1002/arch.22125","DOIUrl":"10.1002/arch.22125","url":null,"abstract":"Insect pest control can be achieved by the application of RNA interference (RNAi), a key molecular tool in functional genomics. Whereas most RNAi research has focused on insect pests, few studies have been performed on natural enemies. Validating the efficacy of RNAi in natural enemies is crucial for assessing its safety and enabling molecular research on these organisms. Here, we assessed the efficacy of RNAi in the ladybird beetle Eriopis connexa Germar (Coleoptera: Coccinellidae), focusing on genes related to reproduction, such as vitellogenin (Vg) and its receptor (VgR). In the transcriptome of E. connexa, we found one VgR (EcVgR) and two Vg genes (EcVg1 and EcVg2). These genes have been validated by in silico analyses of functional domains and evolutionary relationships. Five-day-old females were injected with 500 ng/µL of a specific double-stranded RNA (dsRNA) (dsEcVg1, dsEcVg2, or dsEcVgR) for RNAi tests, while nonspecific dsRNA (dsGFP or dsAgCE8.1) was used as a control. Interestingly, dsEcVg2 was able to knockdown both Vg genes, while dsEcVg1 could silence only EcVg1. Additionally, the viability of the eggs was significantly reduced when both Vg genes were knocked down at the same time (after treatment with dsEcVg2 or “dsEcVg1+dsEcVg2”). Ultimately, malformed, nonviable eggs were produced when EcVgR was silenced. Interestingly, no dsRNA treatment had an impact on the quantity of eggs laid. Therefore, the feasibility of RNAi in E. connexa has been confirmed, suggesting that this coccinellid is an excellent Neotropical model for molecular research on natural enemies and for studying RNAi nontarget effects.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving bee feed recipes to safeguard honeybee colonies during times of food scarcity 改进蜜蜂饲料配方，在食物匮乏时期保护蜜蜂群落。

IF 1.5 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-07-07 DOI: 10.1002/arch.22129

Xue Wang, Xing Zheng, Nana Guo, Mingyang Geng, Rongshen Wang, Ting Huang, Quanzhi Ji, Zhenxing Liu, Yazhou Zhao

{"title":"Improving bee feed recipes to safeguard honeybee colonies during times of food scarcity","authors":"Xue Wang, Xing Zheng, Nana Guo, Mingyang Geng, Rongshen Wang, Ting Huang, Quanzhi Ji, Zhenxing Liu, Yazhou Zhao","doi":"10.1002/arch.22129","DOIUrl":"10.1002/arch.22129","url":null,"abstract":"In beekeeping, when natural nectar or pollen sources become limited, it is crucial to provide supplemental bee feed to maintain the viability of the bee colony. This study was conducted during the autumn food shortage season, during which bees were fed with different proportions of modified bee feed. We identified an optimal bee diet by evaluating honeybee longevity, food consumption, body weight, and gut microbe distribution, with natural pollen serving as a control diet. The results indicated that bees preferred a mixture of 65% defatted soy flour, 20% corn protein powder, 13% wheat germ flour, 2% yeast powder, and a 50% sucrose solution. This bee food recipe significantly increased the longevity, feed consumption, and body weight of bees. The group fed the natural pollen diet exhibited a greater abundance of essential intestinal bacteria. The bee diets used in this study contained higher protein levels and lower concentrations of unsaturated fatty acids and vitamins than did the diets stored within the colonies. Therefore, we propose that incorporating both bee feed and natural pollen in beekeeping practices will achieve more balanced nutritional intake.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap-sucking insects 棉蚜唾液腺转录组学及其与其他吸食汁液昆虫的比较分析

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-06-11 DOI: 10.1002/arch.22123

Shanmugasundram Pavithran, Marimuthu Murugan, Jayakanthan Mannu, Chakkarai Sathyaseelan, Venkatasamy Balasubramani, Sankarasubramanian Harish, Senthil Natesan

{"title":"Salivary gland transcriptomics of the cotton aphid Aphis gossypii and comparative analysis with other sap-sucking insects","authors":"Shanmugasundram Pavithran, Marimuthu Murugan, Jayakanthan Mannu, Chakkarai Sathyaseelan, Venkatasamy Balasubramani, Sankarasubramanian Harish, Senthil Natesan","doi":"10.1002/arch.22123","DOIUrl":"10.1002/arch.22123","url":null,"abstract":"Aphids are sap-sucking insects responsible for crop losses and a severe threat to crop production. Proteins in the aphid saliva are integral in establishing an interaction between aphids and plants and are responsible for host plant adaptation. The cotton aphid, Aphis gossypii (Hemiptera: Aphididae) is a major pest of Gossypium hirsutum. Despite extensive studies of the salivary proteins of various aphid species, the components of A. gossypii salivary glands are unknown. In this study, we identified 123,008 transcripts from the salivary gland of A. gossypii. Among those, 2933 proteins have signal peptides with no transmembrane domain known to be secreted from the cell upon feeding. The transcriptome includes proteins with more comprehensive functions such as digestion, detoxification, regulating host defenses, regulation of salivary glands, and a large set of uncharacterized proteins. Comparative analysis of salivary proteins of different aphids and other insects with A. gossypii revealed that 183 and 88 orthologous clusters were common in the Aphididae and non-Aphididae groups, respectively. The structure prediction for highly expressed salivary proteins indicated that most possess an intrinsically disordered region. These results provide valuable reference data for exploring novel functions of salivary proteins in A. gossypii with their host interactions. The identified proteins may help develop a sustainable way to manage aphid pests.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 2","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and application of carrier-free and carrier-based nanopesticides in pest management 无载体和基于载体的纳米杀虫剂的制造和在害虫防治中的应用。

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-06-11 DOI: 10.1002/arch.22124

Wenhao Dong, Yipeng Ren, Huaijun Xue

引用次数: 0

CRISPR/Cas9 mediated validation of spermatogenesis-related gene, tssk2 as a component of genetic pest management of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) CRISPR/Cas9介导的精子发生相关基因 tssk2 的验证，作为秋季军虫 Spodoptera frugiperda (J. E. Smith) （鳞翅目：夜蛾科）遗传害虫管理的一个组成部分。

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-05-23 DOI: 10.1002/arch.22121

Cholenahalli Narayanappa Anu, Karuppannasamy Ashok, Chikmagalur Nagaraja Bhargava, Yogi Dhawane, Maligeppagol Manamohan, Grish Kumar Jha, Ramasamy Asokan

{"title":"CRISPR/Cas9 mediated validation of spermatogenesis-related gene, tssk2 as a component of genetic pest management of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)","authors":"Cholenahalli Narayanappa Anu, Karuppannasamy Ashok, Chikmagalur Nagaraja Bhargava, Yogi Dhawane, Maligeppagol Manamohan, Grish Kumar Jha, Ramasamy Asokan","doi":"10.1002/arch.22121","DOIUrl":"10.1002/arch.22121","url":null,"abstract":"Invasive insect pests, currently, pose a serious economic threat to several staple crops all over the world, one such being the fall armyworm, Spodoptera frugiperda. It was first observed in Africa since 2016, outside of its natural habitat in the Americas. Subsequently, it invaded several countries in South and South East Asia and also very recently in Australia. In all the newly invaded regions, maize is the principal crop attacked causing a serious economic concern to the poor farmers, particularly in the developing countries. Owing to the innate genetic ability, it defies many of the management options that include insecticides, Bt transgenics, and so forth. This is due to its high mobility, polyphagy and ability for quick development of resistance to several classes of insecticides. At this critical juncture, CRISPR/Cas9 mediated genome editing has shown a lot of promise in developing a novel area-wide pest management strategy called precision-guided sterile insect technique (pgSIT). pgSIT was initially demonstrated in Drosophila melanogaster which holds a greater promise for the environmentally friendly management of several globally significant agricultural pests such as S. frugiperda. Therefore, before developing both sgRNA and Cas9 transgenic lines, we have validated the target gene such as tssk2 through a non-transgenic approach by microinjecting ribo nucleo protein complex (Cas9 protein and tssk2 sgRNA) into G0 eggs of S. frugiperda. In the current investigation, we have obtained five edited males with distinct mutations which were further used for crossing studies to ascertain the effect of tssk2 editing affecting egg hatchability.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The zona pellucida protein piopio regulates the metamorphosis and reproduction in Tribolium castaneum 透明带蛋白 piopio 调节蓖麻蒺藜的变态和繁殖。

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-05-23 DOI: 10.1002/arch.22122

Ling Zhang, Runting Ge, Yanhua Yang, Keping Chen, Chengjun Li

{"title":"The zona pellucida protein piopio regulates the metamorphosis and reproduction in Tribolium castaneum","authors":"Ling Zhang, Runting Ge, Yanhua Yang, Keping Chen, Chengjun Li","doi":"10.1002/arch.22122","DOIUrl":"10.1002/arch.22122","url":null,"abstract":"The zona pellucida domain protein piopio (Pio) was only reported to mediate the adhesion of the apical epithelial surface and the overlying apical extracellular matrix in Drosophila melanogaster, but the developmental roles of Pio were poorly understood in insects. To address this issue, we comprehensively analyzed the function of Pio in Tribolium castaneum. Phylogenetic analysis indicated that pio exhibited one-to-one orthologous relationship among insects. T. castaneum pio had a 1236-bp ORF and contained eight exons. During development pio was abundantly expressed from larva to adult and lowly expressed at the late stage of embryo and adult, while it had more transcripts in the head, epidermis, and gut but fewer in the fat body of late-stage larvae. Knockdown of pio inhibited the pupation, eclosion, and reproduction of T. castaneum. The expression of vitellogenin 1 (Vg1), Vg2, and Vg receptor (VgR) largely decreased in pio-silenced female adults. Silencing pio increased the 20-hydroxyecdysone titer by upregulating phm and spo expression but decreased the juvenile hormone (JH) titer through downregulating JHAMT3 and promoting JHE, JHEH-r4, and JHDK transcription. These results suggested that Pio might regulate the metamorphosis and reproduction via modulating the ecdysone and JH metabolism in T. castaneum. This study found the novel roles of pio in insect metamorphosis and reproduction, and provided the new insights for analyzing other zona pellucida proteins functions in insects.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and indoor virulence determination of a temperature-sensitive insecticide against Zeugodacus cucurbitae (Coquillett) 制备一种对温度敏感的杀虫剂并测定其室内毒力，以对抗 Zeugodacus cucurbitae (Coquillett).

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-05-21 DOI: 10.1002/arch.22115

Aqiang Wang, Chengcheng Ni, Sihua Peng, Shuyan Yang, Qianxing Wu, Shihao Zhou

{"title":"Preparation and indoor virulence determination of a temperature-sensitive insecticide against Zeugodacus cucurbitae (Coquillett)","authors":"Aqiang Wang, Chengcheng Ni, Sihua Peng, Shuyan Yang, Qianxing Wu, Shihao Zhou","doi":"10.1002/arch.22115","DOIUrl":"10.1002/arch.22115","url":null,"abstract":"Zeugodacus cucurbitae (Coquillett) is an important fruit and vegetable pest, especially in high-temperature seasons. In our previous research, we developed a temperature-sensitive sustained-release attractant for Z. cucurbitae, that not only can control the release rate of cuelure according to the temperature change, but also shows an excellent trapping effect on Z. cucurbitae. To further enhance the killing effect of the temperature-sensitive attractant on Z. cucurbitae, this study proposed using it in combination with an insecticide to prepare a temperature-sensitive insecticide for Z. cucurbitae. Based on the controlled release technology of pesticides, a temperature-sensitive Z. cucurbitae insecticide was developed by using PNIPAM gel as a temperature-sensitive switch to carry both cuelure and insecticide at the same time. In addition, the lethal effect of different pesticides on Z. cucurbitae were tested by indoor toxicity test, and the best pesticide combination was screened out. The temperature-sensitive insecticide prepared in this study not only had excellent thermal response and controlled release ability, but also enhanced its toxicological effects on Z. cucurbitae because it contained insecticides. Among them, combining thiamethoxam and clothianidin with the temperature-sensitive attractants was the most effective, and their lethality reached more than 97% against Z. cucurbitae. This study is not only of great practical significance for the monitoring and controlling Z. cucurbitae, but also provides theoretical basis and reference value for the combination of temperature-sensitive attractant and insecticide.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and function of the vitellogenin receptor in the hypopharyngeal glands of the honey bee Apis mellifera (Hymenoptera: Apidae) workers 蜜蜂工蜂下咽腺中卵黄素受体的表达和功能。

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-05-13 DOI: 10.1002/arch.22120

Virginia Teles Dohanik, Luanda Medeiros-Santana, Carolina Gonçalves Santos, Weyder Cristiano Santana, José Eduardo Serrão

{"title":"Expression and function of the vitellogenin receptor in the hypopharyngeal glands of the honey bee Apis mellifera (Hymenoptera: Apidae) workers","authors":"Virginia Teles Dohanik, Luanda Medeiros-Santana, Carolina Gonçalves Santos, Weyder Cristiano Santana, José Eduardo Serrão","doi":"10.1002/arch.22120","DOIUrl":"10.1002/arch.22120","url":null,"abstract":"The vitellogenin receptor (VgR) is essential for the uptake and transport of the yolk precursor, vitellogenin (Vg). Vg is synthesized in the fat body, released in the hemolymph, and absorbed in the ovaries, via receptor-mediated endocytosis. Besides its important role in the reproductive pathway, Vg occurs in nonreproductive worker honey bee, suggesting its participation in other pathways. The objective was to verify if the VgR occurs in the hypopharyngeal glands of Apis mellifera workers and how Vg is internalized by these cells. VgR occurrence in the hypopharyngeal glands was evaluated by qPCR analyses of VgR and immunohistochemistry in workers with different tasks. The VgR gene is expressed in the hypopharyngeal glands of workers with higher transcript levels in nurse honey bees. VgR is more expressed in 11-day-old workers from queenright colonies, compared to orphan ones. Nurse workers with developed hypopharyngeal glands present higher VgR transcripts than those with poorly developed glands. The immunohistochemistry results showed the co-localization of Vg, VgR and clathrin (protein that plays a major role in the formation of coated vesicles in endocytosis) in the hypopharyngeal glands, suggesting receptor-mediated endocytosis. The results demonstrate that VgR performs the transport of Vg to the hypopharyngeal glands, supporting the Ovary Ground Plan Hypothesis and contributing to the understanding of the role of this gland in the social context of honey bees.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpha-ketoglutarate supplementation in long-lived Drosophila melanogaster: Impact on lifespan and metabolic responses 在长寿果蝇中补充α-酮戊二酸：对寿命和代谢反应的影响

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-05-13 DOI: 10.1002/arch.22116

Oleh Demianchuk, Maria Lylyk, Vitalii Balatskiy, Dmytro Gospodaryov, Maria Bayliak

{"title":"Alpha-ketoglutarate supplementation in long-lived Drosophila melanogaster: Impact on lifespan and metabolic responses","authors":"Oleh Demianchuk, Maria Lylyk, Vitalii Balatskiy, Dmytro Gospodaryov, Maria Bayliak","doi":"10.1002/arch.22116","DOIUrl":"10.1002/arch.22116","url":null,"abstract":"Studies on antiaging remedies in insect models sometimes show discrepancies in results. These discrepancies could be explained by different responses of short- and long-lived strains on the antiaging remedies. The purpose of the study was to test whether life-prolonging effects of alpha-ketoglutarate (AKG), observed in nematodes and fruit flies, would be reproduced in long-lived Drosophila melanogaster flies. Lifespan was assayed in flies kept in demographic cages. Fecundity, proportion of flies capable of negative geotaxis, starvation resistance, time of heat coma onset, levels of triacyglycerols, body glucose, glycogen, activities of glutamate dehydrogenase, catalase, glutathione-S-transferase, hexokinase, phosphofructokinase, pyruvate kinase, lactate, and glutamate dehydrogenases were assessed. Dietary AKG did not affect fly lifespan on the diet with 5% yeast and 5% sucrose (5Y:5S) and on the diet with 9% yeast and 1% sucrose (9Y:1S), but increased lifespan on the low-protein diet (1Y:9S). Twenty-five-day-old female flies fed a 5Y:5S diet with 10 mM AKG for 3 weeks, did not differ from the control group (without AKG) in climbing activity, resistance to heat stress, and starvation. The levels of glucose and glycogen were unaffected but the levels of triacylglycerols were lower in AKG-fed female flies. No differences in activities of glycolytic enzymes, NADPH-producing enzymes, glutamate dehydrogenase, oxygen consumption, and levels of oxidative stress markers were observed between the control and AKG-fed flies. However, AKG-fed flies had lower activities of catalase and glutathione-S-transferase. These results suggest that potential antiaging remedies, such as AKG, may not extend lifespan in long-living organisms despite influencing several metabolic parameters.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization of Bombyx mori Dicer-2 that dices double-stranded RNAs into 20-nt small RNA 将双链 RNA 分解为 20-nt 小 RNA 的森蚕蛾 Dicer-2 的生化特征。

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-05-07 DOI: 10.1002/arch.22118

Midori Tabara, Mayuko Harada, Kazunori Kuriyama, Takuma Sakamoto, Atsushi Takeda, Toshiyuki Fukuhara, Hiroko Tabunoki

引用次数: 0