Archives of Insect Biochemistry and Physiology最新文献_第9页

Identification of long noncoding RNAs of silkworm at the early stage of Bombyx mori bidensovirus infection 家蚕标本病毒感染早期长非编码 RNA 的鉴定

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-24 DOI: 10.1002/arch.22082

Yeping Chen, Zihe Wang, Chengyue Wu, Hao Li, Heying Qian, Mengdong Wang, Ping Wu, Xijie Guo, Zhendong Zhang

{"title":"Identification of long noncoding RNAs of silkworm at the early stage of Bombyx mori bidensovirus infection","authors":"Yeping Chen, Zihe Wang, Chengyue Wu, Hao Li, Heying Qian, Mengdong Wang, Ping Wu, Xijie Guo, Zhendong Zhang","doi":"10.1002/arch.22082","DOIUrl":"10.1002/arch.22082","url":null,"abstract":"Bombyx mori bidensovirus (BmBDV) is one of the most important pathogens of silkworm. It mainly infects midgut cells of silkworm and causes losses to the sericulture industry. Long noncoding RNAs (lncRNAs) have been reported to play an important role in the regulation of antiviral immune response in silkworm. To explore whether lncRNAs are involved in BmBDV infection and immune response of silkworm, we performed a comparative transcriptome analysis to identify the lncRNAs and mRNAs between the BmBDV infected and noninfected silkworm larvae at the early stage. A total of 16,069 genes and 974 candidate lncRNAs were identified, among which 142 messenger RNA (mRNAs) and four lncRNAs were differentially expressed (DE). Target gene prediction revealed that 142 DEmRNAs were coexpressed with four DElncRNAs, suggesting that the expression of mRNA is mainly affected through trans-regulation activities. A regulatory network of DElncRNAs and DEmRNAs was constructed, showing that many genes targeted by different DElncRNAs are involved in metabolism and immunity, which implies that these genes and lncRNAs play an important role in the replication of BmBDV. Our results will help us to improve our understanding of lncRNA-mediated regulatory roles in BmBDV infection, providing a new perspective for further exploring the interaction between host and BmBDV.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The extraordinary rearrangement of mitochondrial genome of the wheat pest, Aptinothrips stylifer and the mitochondrial phylogeny of Thripidae (Thysanoptera) 小麦害虫 Aptinothrips stylifer 线粒体基因组的非凡重排和蓟马科线粒体系统发育

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-24 DOI: 10.1002/arch.22086

Chengwen Li, Yuxin Gao, Dongxue Wang, Lihong Dang

{"title":"The extraordinary rearrangement of mitochondrial genome of the wheat pest, Aptinothrips stylifer and the mitochondrial phylogeny of Thripidae (Thysanoptera)","authors":"Chengwen Li, Yuxin Gao, Dongxue Wang, Lihong Dang","doi":"10.1002/arch.22086","DOIUrl":"10.1002/arch.22086","url":null,"abstract":"The mitochondrial gene order in Thysanoptera is notably distinct and highly rearranged, with each species exhibiting its own unique arrangement. To elucidate the relationship between gene rearrangements and phylogeny, the complete mitochondrial genome (mitogenome) of the wheat pest, Aptinothrips stylifer, was sequenced and assembled, spanning a total length of 16,033 bp. Compared with the ancestral arthropod mitogenome, significant rearrangement differences were evident in A. stylifer, whereas the gene order between A. stylifer and Anaphothrips obscurus was similar. Phylogenetic trees were reconstructed based on all 13 protein-coding gene sequences using Bayesian inference and maximum-likelihood methods, both yielding similar topological structures. Notably, A. stylifer was robustly clustered with A. obscurus, affirming its classification within Anaphothrips genus group. This exemplifies the potential correlation between gene rearrangements and phylogeny in the Thripidae family. Additionally, the mitogenome of A. stylifer exhibited several atypical features, including: (1) Three putative control regions (CRs) in close proximity, with CR2 and CR3 displaying partial similarity, and CR1 differing in base composition; (2) Two transfer RNAs (tRNAs), trnS1 and trnV, lacking the DHU arm; (3) Two ribosomal RNA (rRNA) genes inverted and positioned distant from each other; (4) Negative AT and GC skew (AT skew = −0.001, GC skew = −0.077); (5) One transposition (nad6), one inverse transposition (trnQ), four inversions (trnF, trnH, trnC, and gene block nad1-trnL1-rrnL-trnV-rrnS), and four tandem duplication random loss events; and (6) Two protein-coding genes, nad2 and atp8, terminated with an incomplete stop codon “T”.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of amino acid enriched diets on hemolymph amino acid composition in honey bees 富含氨基酸的日粮对蜜蜂血淋巴氨基酸组成的影响

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-24 DOI: 10.1002/arch.22085

Elena Tafi, Simona Sagona, Valentina Meucci, Laura Bortolotti, Marta Galloni, Gherardo Bogo, Domenico Gatta, Lucia Casini, Marta Barberis, Massimo Nepi, Antonio Felicioli

{"title":"Effect of amino acid enriched diets on hemolymph amino acid composition in honey bees","authors":"Elena Tafi, Simona Sagona, Valentina Meucci, Laura Bortolotti, Marta Galloni, Gherardo Bogo, Domenico Gatta, Lucia Casini, Marta Barberis, Massimo Nepi, Antonio Felicioli","doi":"10.1002/arch.22085","DOIUrl":"10.1002/arch.22085","url":null,"abstract":"Amino acids (AAs) are an abundant class of nectar solutes, and they are involved in the nectar attractiveness to flower visitors. Among the various AAs, proline is the most abundant proteogenic AA, and γ-amino butyric acid (GABA) and β-alanine are the two most abundant non-proteogenic AAs. These three AAs are known to affect insect physiology, being involved in flight metabolism and neurotransmission. The aim of this study was to investigate the effects of artificial diets enriched with either β-alanine, GABA, or proline on consumption, survival, and hemolymph composition in honey bees belonging to two different ages and with different metabolism (i.e., newly emerged and foragers). Differences in feed intake among diets were not observed, while a diet enriched with β-alanine improved the survival rate of newly emerged honey bees compared to the control group. Variations in the hemolymph AA concentrations occurred only in newly emerged honey bees, according to the diet and the time of hemolymph sampling. A greater susceptibility of young honey bees to enriched diets than older honey bees was observed. The variations in the concentrations of hemolymph AAs reflect either the accumulation of dietary AAs or the existence of metabolic pathways that may lead to the conversion of dietary AAs into different ones. This investigation could be an initial contribution to studying the complex dynamics that regulate hemolymph AA composition and its effect on honey bee physiology.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of diabetes mellitus model using Bombyx mori silkworms in a low-temperature environment 利用低温环境下的桑蚕建立糖尿病模型

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-24 DOI: 10.1002/arch.22083

Jiaming Hou, Cheng Tan, Nan Chen, Yuan Zhou, Shaojun Huang, Huani Chen, Li Qian

{"title":"Establishment of diabetes mellitus model using Bombyx mori silkworms in a low-temperature environment","authors":"Jiaming Hou, Cheng Tan, Nan Chen, Yuan Zhou, Shaojun Huang, Huani Chen, Li Qian","doi":"10.1002/arch.22083","DOIUrl":"10.1002/arch.22083","url":null,"abstract":"Due to the high prevalence of diabetes mellitus, researchers have conducted numerous experimental animal studies. However, the mammalian diabetes model is cumbersome and expensive to operate, while the cheap and simple common silkworm diabetes model has the disadvantage of a short cycle time. Since the growth of silkworms is greatly affected by environmental factors, we extended the five-age cycle of silkworms by lowering the ambient temperature to establish a novel low-temperature silkworm diabetes model. Our goal was to determine whether the low-temperature feeding of a high-sugar diet to silkworms could serve as an effective animal model for diabetes. Also, we aimed to resolve certain issues concerning the normal temperature silkworm diabetes model, such as the short time frame for experiments and erratic fluctuations in blood sugar levels. Silkworms weighing between 0.9 and 1.0 g at the beginning of the fifth instar were selected, and we created diabetic silkworms by feeding mulberry leaves containing 4% glucose daily in a 16–20°C environment. When the silkworms were kept at a cooler temperature, the fifth instar stage lasted for an additional 9–11 days. In the model group, 83.3% of the silkworms had blood glucose levels greater than 7.8 mmol/L, while the total prevalence of diabetic silkworms was 89.8%. Moreover, JNK phosphorylation expression rose in the model group, while PI3K expression fell. Additionally, the JNK and PI3K signaling pathway expressions matched diabetic signals. Therefore, using silkworms to create a diabetes model in a cool environment is a straightforward and cost-effective approach to studying diabetes in animals.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139556631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of insect immune suppressors using a recombinant phospholipase A2 of a lepidopteran insect 利用鳞翅目昆虫的重组磷脂酶 A2 筛选昆虫免疫抑制剂

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-11 DOI: 10.1002/arch.22081

Gahyeon Jin, Yonggyun Kim

{"title":"Screening of insect immune suppressors using a recombinant phospholipase A2 of a lepidopteran insect","authors":"Gahyeon Jin, Yonggyun Kim","doi":"10.1002/arch.22081","DOIUrl":"https://doi.org/10.1002/arch.22081","url":null,"abstract":"Phospholipase A2 (PLA2) catalyzes phospholipids at the sn-2 position to release free fatty acids, including arachidonic acid (AA) or its precursor. The free AA is then oxygenated into different eicosanoids, which mediate the diverse physiological processes in insects. Any inhibition of the PLA2 catalysis would give rise to serious malfunctioning in insect growth and development. An onion moth, Acrolepiopsis sapporensis, encodes four different PLA2 genes (As-PLA2A–As-PLA2D), in which As-PLA2A is dominantly expressed at all developmental stages and in different larval tissues. RNA interference of the As-PLA2A expression significantly reduced the PLA2 activity of A. sapporensis, which suffered from immunosuppression. A recombinant As-PLA2A protein was purified from a bacterial expression system, which exhibited a typical Michaelis—Menten kinetics and hence susceptible to a specific inhibitor to sPLA2 and dithiothreitol. A total of 19 bacterial metabolites derived from Xenorhabdus and Photorhabdus were screened against the recombinant As-PLA2A. Five potent metabolites were highly inhibitory and followed a competitive enzyme inhibition. These five inhibitors suppressed the immune responses of A. sapporensis by inhibiting hemocyte-spreading behavior and phenoloxidase activity. However, an addition of AA could significantly rescue the immunosuppression induced by the selected inhibitors. These studies suggest that the recombinant As-PLA2A protein can be applied for high-throughput screening of insect immunosuppressive compounds.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139435118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single domain von Willebrand factor type C “cytokines” and the regulation of the stress/immune response in insects 单域冯-威廉因子 C 型 "细胞因子 "与昆虫应激/免疫反应的调控

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-08 DOI: 10.1002/arch.22071

Vassiliki Labropoulou, Luoluo Wang, Christiana Magkrioti, Guy Smagghe, Luc Swevers

{"title":"Single domain von Willebrand factor type C “cytokines” and the regulation of the stress/immune response in insects","authors":"Vassiliki Labropoulou, Luoluo Wang, Christiana Magkrioti, Guy Smagghe, Luc Swevers","doi":"10.1002/arch.22071","DOIUrl":"10.1002/arch.22071","url":null,"abstract":"The single domain von Willebrand factor type C (SVWC) appears in small secreted peptides that are arthropod-specific and are produced following environmental stress or pathogen exposure. Most research has focused on proteins with SVWC domain that are induced after virus infection and are hypothesized to function as “cytokines” to regulate the innate immune response. The expansion of SVWC genes in insect species indicates that many other functions remain to be discovered. Research in shrimp has elucidated the adaptability of Vago-like peptides in the innate immune response against bacteria, fungi and viruses after activation by Jak-STAT and/or Toll/Imd pathways in which they can act as pathogen-recognition receptors or cytokine-like signaling molecules. SVWC factors also appear in scorpion venoms and tick saliva, underlining their versatility to acquire new functions. This review discusses the discovery and function of SVWC peptides from insects to crustaceans and chelicerates and reveals the enormous gaps in knowledge that remain to be filled to understand this enigmatic group of secreted peptides.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/arch.22071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139397307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

K64 acetylation of heat shock protein 90 suppresses nucleopolyhedrovirus replication in Bombyx mori 热休克蛋白 90 的 K64 乙酰化可抑制核多角体病毒在桑蚕体内的复制

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-05 DOI: 10.1002/arch.22079

Xizhen Zhang, Shiyi Ma, Chaoguang Gu, Miao Hu, Meng Miao, Yanping Quan, Wei Yu

{"title":"K64 acetylation of heat shock protein 90 suppresses nucleopolyhedrovirus replication in Bombyx mori","authors":"Xizhen Zhang, Shiyi Ma, Chaoguang Gu, Miao Hu, Meng Miao, Yanping Quan, Wei Yu","doi":"10.1002/arch.22079","DOIUrl":"https://doi.org/10.1002/arch.22079","url":null,"abstract":"HSP90 is a highly conserved chaperone that facilitates the proliferation of many viruses, including silkworm (bombyx mori) nucleopolyhedrovirus (BmNPV), but the underlying regulatory mechanism was unclear. We found that suppression of HSP90 by 17-AAG, a HSP90-specific inhibitor, significantly reduced the expression of BmNPV capsid protein gp64 and viral genome replication, whereas overexpression of B. mori HSP90(BmHSP90) promoted BmNPV replication. Furthermore, in a recent study of the lysine acetylome of B. mori infected with BmNPV, we focused on the reduced viral proliferation due to changes of BmHSP90 lysine acetylation. Site-directed introduction of acetylated (K/Q) or deacetylated (K/R) mimic mutations into BmHSP90 revealed that lysine 64 (K64) acetylation activated the JAK/STAT pathway and reduced BmHSP90 ATPase activity, leading to diminished chaperone activity and ultimately inhibiting BmNPV proliferation. In this study, a single lysine 64 acetylation change of BmHSP90 was elucidated as a model of posttranslational modifications occurring in the wake of host-virus interactions, providing novel insights into potential antiviral strategies.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different development and fecundity between Spodoptera frugiperda USA and China populations, influenced by ecdysone-related genes 受蜕皮激素相关基因影响，美国和中国种群的鞘翅目昆虫发育和繁殖力不同

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-05 DOI: 10.1002/arch.22074

Du-Yeol Choi, Hyoung-ho Mo, Youngjin Park

{"title":"Different development and fecundity between Spodoptera frugiperda USA and China populations, influenced by ecdysone-related genes","authors":"Du-Yeol Choi, Hyoung-ho Mo, Youngjin Park","doi":"10.1002/arch.22074","DOIUrl":"https://doi.org/10.1002/arch.22074","url":null,"abstract":"The fall armyworm (FAW), Spodoptera frugiperda, is one of the most harmful plant pests in the world and is globally distributed from the American continent to the Asian region. The FAW USA population (Sf-USA) and China population (Sf-CHN), which belong to corn strain, showed different developmental periods and fecundity rates in lab conditions. Sf-USA had faster development and higher fecundity compared with Sf-CHN. To examine these differences, transcriptomic data from two FAW populations were analyzed and compared. Twelve gigabytes of transcripts were read from each sample and 21,258 differentially expressed genes (DEGs) were detected. DEGs with log2 fold change ≥ 2 were identified and compared in two populations. In comparison to the Sf-CHN, we discovered that 3471 and 3851 individual DEGs upregulated and downregulated, respectively. Comparing transcriptome profiles for differential gene expression revealed several DEGs, including 39 of ecdysone (E)-, 25 of juvenile hormone-, and 15 of insulin-related genes. We selected six of E-related genes, such as Neverland, Shade, Ecdysone receptor, Ecdysone-inducible protein 74 (E74), E75, and E78 from DEGs. Gene expressions were suppressed by RNA interference to confirm the physiological functions of the selected genes from Sf-USA. The Sf-USA showed developmental retardation and a decrease in fecundity rate by suppression of E-related genes. These findings show that biological characteristics between Sf-USA and Sf-CHN are influenced by E-related genes.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA barcoding of the genus Gampsocleis (Orthoptera, Tettigoniidae) from China 中国 Gampsocleis 属（直翅目，Tettigoniidae）的 DNA 条形编码

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-05 DOI: 10.1002/arch.22070

Pu Zhang, Yuting Cai, Lan Ma, Jinyan Chai, Zhijun Zhou

{"title":"DNA barcoding of the genus Gampsocleis (Orthoptera, Tettigoniidae) from China","authors":"Pu Zhang, Yuting Cai, Lan Ma, Jinyan Chai, Zhijun Zhou","doi":"10.1002/arch.22070","DOIUrl":"https://doi.org/10.1002/arch.22070","url":null,"abstract":"DNA barcoding is a useful addition to the traditional morphology-based taxonomy. A ca. 650 bp fragment of the 5′ end of mitochondrial cytochrome c oxidase subunit I (hereafter COI-5P) DNA barcoding was sued as a practical tool for Gampsocleis species identification. DNA barcodes from 889 specimens belonging to 8 putative Gampsocleis species was analyzed, including 687 newly generated DNA barcodes. These barcode sequences were clustered/grouped into Operational Taxonomic Units (OTUs) using the criteria of five algorithms, namely Barcode Index Number (BIN) System, Assemble Species by Automatic Partitioning (ASAP), a Java program uses an explicit, determinate algorithm to define Molecular Operational Taxonomic Unit (jMOTU), Generalized Mixed Yule Coalescent (GMYC), and Bayesian implementation of the Poisson Tree Processes model (bPTP). The Taxon ID Tree grouped sequences of morphospecies and almost all MOTUs in distinct nonoverlapping clusters. Both long- and short-winged Gampsocleis species are reciprocally monophyletic in the Taxon ID Tree. In BOLD, 889 barcode sequences are assigned to 17 BINs. The algorithms ASAP, jMOTU, bPTP and GMYC clustered the barcode sequences into 6, 13, 10, and 23 MOTUs, respectively. BIN, ASAP, and bPTP algorithm placed three long-winged species, G. sedakovii, G. sinensis and G. ussuriensis within the same MOTU. All species delimitation algorithms split two short-winged species,G. fletcheri and G. gratiosa into at least two MOTUs each, except for ASAP algorithm. More detailed molecular and morphological integrative studies are required to clarify the status of these MOTUs in the future.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139110043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecdysone and gene expressions for chromatin remodeling, histone modification, and Broad Complex in relation to pupal commitment in Bombyx mori 蜕皮激素与染色质重塑、组蛋白修饰和 Broad Complex 的基因表达，以及与森双翅目昆虫蛹的形成有关的关系

IF 2.2 4区农林科学

Archives of Insect Biochemistry and Physiology Pub Date : 2024-01-02 DOI: 10.1002/arch.22076

Maki Saito, Shota Fujimoto, Hideki Kawasaki

{"title":"Ecdysone and gene expressions for chromatin remodeling, histone modification, and Broad Complex in relation to pupal commitment in Bombyx mori","authors":"Maki Saito, Shota Fujimoto, Hideki Kawasaki","doi":"10.1002/arch.22076","DOIUrl":"10.1002/arch.22076","url":null,"abstract":"In the present study, we tried to clarify when and how pupal commitment (PT) better to use PC occurs and what is involved in the PT of Bombyx mori. To clarify this, we examined the responsiveness of a wing disc to ecdysone, referring to metamorphosis-related BR-C, development-related Myc and Wnt, and chromatin remodeling-related genes at around the predicted PT stage of the Bombyx wing disc. Wing disc responsiveness to juvenile hormone (JH) and ecdysone was examined using Methoprene and 20-hydroxyecdysone (20E) in vitro. The body weight of B. mori increased after the last larval ecdysis, peaked at Day 5 of the fifth larval instar (D5L5), and then decreased. The responsiveness of the wing disc to JH decreased after the last larval ecdysis up to D3L5. Bmbr-c (the Broad Complex of B. mori) showed enhanced expression in D4L5 wing discs with 20E treatment. Some chromatin remodeler and histone modifier genes (Bmsnr1, Bmutx, and Bmtip60) showed upregulation after being cultured with 20E in D4L5 wing discs. A low concentration of 20E is suggested to induce responsiveness to 20E in D4L5 wing discs. Bmbr-c, Bmsnr1, Bmutx, and Bmtip60 were upregulated after being cultured with a low concentration of 20E in D4L5 wing discs. The expression of Bmmyc and Bmwnt1 did not show a change after being cultured with or without 20E in D4L5 wing discs, while enhanced expression was observed with 20E in D5L5 wing discs. From the present results, we concluded that PT of the wing disc of B. mori occurred beginning on D4L5 with the secretion of low concentrations of ecdysteroids. Bmsnr1, Bmutx, Bmtip60, and BR-C are also involved.","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"115 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139077445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0