Archives of Insect Biochemistry and Physiology最新文献

{"title":"Fitness costs, realized heritability, and mechanism of resistance to tenvermectin B in Plutella xylostella","authors":"Kui Zhang,&nbsp;Wenwu Rao,&nbsp;Linying Zhu,&nbsp;Shaoyong Zhang,&nbsp;Jie Chen,&nbsp;Sha Zhou,&nbsp;Anliang Chen","doi":"10.1002/arch.22144","DOIUrl":"10.1002/arch.22144","url":null,"abstract":"<p>Tenvermectin B (TVM-B) and five TVM-B analogs were produced by fermentation of a genetically engineered strain <i>Streptomyces avermitilis</i> HU02, and TVM-B is being developed as a new insecticide. Through 11 generations of resistance selection against TVM-B in the diamondback moth, <i>Plutella xylostella</i>, the median lethal concentration (LC₅₀) was increased from 14.84 to 1213.73 mg L⁻¹. The resistance to TVM-B in <i>P. xylostella</i> developed fast and its realized heritability was high (<i>h</i>² = 0.2901 (F7), <i>h</i>² = 0.4070 (F11)). However, the relative fitness was 0.6916 suggesting a fitness cost in the resistant strains. The fitness cost was partially explained by the upregulation of the detoxification enzyme activity by 2.15 folds in carboxylate esterase (CarE) and the gene expressions of ATP-binding cassette transporter gene (<i>ABCC2</i>) and the alpha subunit of the glutamate-gated chloride channel (GluCl) by 1.70- and 2.32 folds, respectively. The resistance was also explained by two points of mutations at the alpha subunit of the glutamate-gated chloride channel in the <i>P. xylostella</i> (<i>Px</i>GluCl<i>α</i>) subunit in F11. However, there was little change in the binding affinity. These results provided helpful information for the mechanism study of TVM-B resistance and will be conducive to designing rational resistance management strategies in <i>P. xylostella</i>.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect cuticular protein; gene expression, genomic structure, transcriptional regulation, speculated cuticular structure, clarified through the genomic analysis of Bombyx mori","authors":"Hideki Kawasaki","doi":"10.1002/arch.22143","DOIUrl":"10.1002/arch.22143","url":null,"abstract":"<p>JH and ecdysone signaling regulate insect metamorphosis through the master transcription factors, Krüppel homolog 1 (kr-h1), Broad-Complex (BR-C), and E93. Ecdysone signaling activates successively expressed ecdysone responsive transcription factors (ERTFs), and the interaction between ERTFs determines the expression profiles of ERTFs themselves. Through the construction of expressed sequence tag (EST) database of <i>Bombyx mori</i> from many tissues, the existence of a large number of cuticular protein (CP) genes was identified in wing disc cDNA library of the 3 days after the start of wandering (W3). From the genomic analysis, 12 types of CP clusters of CP genes were identified. DNA sequences of CP genes revealed the duplication of CP genes, which suggests to reflect the insect evolution. These CP genes responded to ecdysone and ecdysone pulse; therefore, CP genes were applied for the analysis of transcriptional regulation by ERTF. The binding sites of ERTF have been reported to exist upstream of CP genes in several insects, and the activation of CP genes occurred by the binding of ERTFs. Through the analysis, the following were speculated; the successive appearance of ERTFs and the activation of target genes resulted in the successively produced CPs and cuticular layer. The sequence of the ERTF and CP gene expression was the same at larval to pupal and pupal to adult transformation. The involvement of several ERTFs in one CP gene expression was also clarified; <i>BmorCPG12</i> belongs to group showing expression peak at W3 and was regulated by two ERTFs; BHR3 and ßFTZ-F1, <i>BmorCPH2</i> belongs to group showing expression peak at P0 and was regulated by two ERTFs; ßFTZ-F1 and E74A. The involvement of BHR39 as a negative regulator of CP gene expression was found. Larval, pupal, and adult cuticular layers were supposed to be constructed by the combination of different and similar types of CPs, through the expressed timing of CP genes.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of high temperature on the growth performance and midgut autophagy of thermotolerant and thermosensitive silkworm strains","authors":"Huiduo Guo,&nbsp;Mingfa Ling,&nbsp;Runhuan Yang,&nbsp;Tao Gui,&nbsp;Gang Li,&nbsp;Guodong Zhao,&nbsp;Wenbin Wang,&nbsp;Heying Qian","doi":"10.1002/arch.22128","DOIUrl":"10.1002/arch.22128","url":null,"abstract":"<p>High temperature stress has long-term negative effects on the growth and development of silkworm (<i>Bombyx mori</i>). Different silkworm varieties show the different tolerance to high temperature. The induction of autophagy is linked to increased thermotolerance in diverse ectothermic organisms. However, the function of autophagy in the thermotolerant and thermosensitive silkworm strains under high-temperature conditions remains unclear. The thermotolerant Liangguang NO.2 and thermosensitive Jingsong × Haoyue strains were used to explore the role of autophagy in thermotolerance. Here, we first found that the larval body weight gain was increased in the thermosensitive Jingsong × Haoyue strain, but there was no difference in the thermotolerant Liangguang NO.2 strain under high temperature conditions. High temperature stress had a negative influence on the cocoon performance in both the Liangguang NO.2 and Jingsong × Haoyue strains. Additionally, the autophagy-related gene <i>Atg5</i> mRNA expression in the Liangguang NO.2 strain was upregulated by high temperature, while the expression of <i>Atg12</i> mRNA was reduced in the Jingsong × Haoyue strain. Titers of 20-Hydroxyecdysone and the ultraspiracle 1 mRNA expression in the Liangguang NO.2 strain were upregulated by high temperature, which might be associated with the induction of autophagy. These results demonstrate the potentially regulatory mechanism of autophagy in silkworms' tolerance to high temperature, providing a theoretical basis for exploring the physiological mechanism of thermotolerance in insects.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional expression and characterization of CAPA receptor in the digestive tract and life stages of Drosophila suzukii, and differential activities with insect PRXamide peptides","authors":"Briana E. Price,&nbsp;Hyo Sang Jang,&nbsp;Ryssa K. Parks,&nbsp;Man-Yeon Choi","doi":"10.1002/arch.22080","DOIUrl":"10.1002/arch.22080","url":null,"abstract":"<p>Spotted-wing drosophila, <i>Drosophila suzukii</i> (Matsumura), is an invasive vinegar fly that is a major threat to the small fruits industries globally. Insect <i>capa</i> genes encode multiple neuropeptides, including CAPA-periviscerokinin (CAPA-PVK) peptides, that are specifically known to cause diuresis or anti-diuresis in various organisms. Here we identified and characterized a corresponding G protein-coupled receptor (GPCR) of the <i>D. suzukii</i> CAPA-PVK peptides: CAPA receptor (CAPA-R). To better characterize the behavior of <i>D. suzukii</i> CAPA-R, we used insect cell-based functional expression assays to evaluate responses of CAPA-R against <i>D. suzukii</i> CAPA-PVKs, CAPA-PVKs from five species in Insecta, one species from Mollusca, modified CAPA-PVK peptides, and some PRXamide family peptides: pyrokinin (PK), diapause hormone (DH), and ecdysis-triggering hormone (ETH). Functional studies revealed that the <i>D. suzukii</i> CAPA-R is strongly activated by both of its own natural <i>D. suzukii</i> CAPA-PVKs, and interestingly, it was strongly activated by other CAPA-PVK peptides from <i>Frankliniella occidentallis</i> (Thysanoptera), <i>Solenopsis invicta</i> (Hymenoptera), <i>Helicoverpa zea</i> (Lepidoptera) and <i>Plutella xylostella</i> (Lepidoptera). However, <i>D. suzukii</i> CAPA-R was not activated by Mollusca CAPA-PVK or the other PRXamide peptides. Gene expression analyses showed that the CAPA-R was highly expressed in the Malpighian tubules and moderately in hindgut compared to other digestive organs or the rest of body, supporting diuretic/antidiuretic functionality. When compared across life stages of <i>D. suzukii</i>, expression of CAPA-R was approximately 1.5x greater in the third instar than the other stages and minimally detected in the eggs, 4-day old pupae and 3-day old adults. Our results functionally characterized the <i>D. suzukii</i> CAPA-R and a few short peptides were identified as potential biological targets to exploit the CAPA-R for <i>D. suzukii</i> management.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dimethyl sulfoxide, an alternative for control of Nosema ceranae infection in honey bees (Apis mellifera)","authors":"Shang-Tse Ho,&nbsp;Yu-Shin Nai,&nbsp;Zih-Ting Chang,&nbsp;Ju-Chun Chang,&nbsp;Wei-Chen Hsu,&nbsp;Chung-Yu Ko,&nbsp;Yue-Wen Chen,&nbsp;Yu-Liang Yang","doi":"10.1002/arch.22099","DOIUrl":"10.1002/arch.22099","url":null,"abstract":"<p><i>Nosema ceranae</i> is a microsporidian parasite that threatens current apiculture. <i>N. ceranae</i>-infected honey bees (<i>Apis mellifera</i>) exhibit morbid physiological impairments and reduced honey production, malnutrition, shorter life span, and higher mortality than healthy honey bees. In this study, we found that dimethyl sulfoxide (DMSO) could enhance the survival rate of <i>N. ceranae</i>-infected honey bees. Therefore, we investigated the effect of DMSO on <i>N. ceranae</i>-infected honey bees using comparative RNA sequencing analysis. Our results revealed that DMSO was able to affect several biochemical pathways, especially the metabolic-related pathways in <i>N. ceranae</i>-infected honey bees. Based on these findings, we conclude that DMSO may be a useful alternative for treating <i>N. ceranae</i> infection in apiculture.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of wild aquatic insect communities to thermal variation through comparative landscape transcriptomics","authors":"Maribet Gamboa,&nbsp;Yusuke Gotoh,&nbsp;Arnelyn Doloiras-Laraño,&nbsp;Kozo Watanabe","doi":"10.1002/arch.22137","DOIUrl":"10.1002/arch.22137","url":null,"abstract":"<p>Fluctuations in temperature are recognized as a potent driver of selection pressure, fostering genomic variations that are crucial for the adaptation and survival of organisms under selection. Notably, water temperature is a pivotal factor influencing aquatic organism persistence. By comprehending how aquatic organisms respond to shifts in water temperature, we can understand their potential physiological adaptations to environmental change in one or multiple species. This, in turn, contributes to the formulation of biologically relevant guidelines for the landscape scale transcriptome profile of organisms in lotic systems. Here, we investigated the distinct responses of seven stream stonefly species, collected from four geographical regions across Japan, to variations in temperature, including atmospheric and water temperatures. We achieved this by assessing the differences in gene expression through RNA-sequencing within individual species and exploring the patterns of community-genes among different species. We identified 735 genes that exhibited differential expressions across the temperature gradient. Remarkably, the community displayed expression levels differences of respiration and metabolic genes. Additionally, the diversity in molecular functions appeared to be linked to spatial variation, with water temperature differences potentially contributing to the overall functional diversity of genes. We found 22 community-genes with consistent expression patterns among species in response to water temperature variations. These genes related to respiration, metabolism and development exhibited a clear gradient providing robust evidence of divergent adaptive responses to water temperature. Our findings underscore the differential adaptation of stonefly species to local environmental conditions, suggesting that shared responses in gene expression may occur across multiple species under similar environmental conditions. This study emphasizes the significance of considering various species when assessing the impacts of environmental changes on aquatic insect communities and understanding potential mechanisms to cope with such changes.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ultraviolet on the environmental adaptability of Megalurothrips usitatus (Thysanoptera: Thripidae)","authors":"Haifeng Jin,&nbsp;Chaozheng Wang,&nbsp;Wayne Jiang,&nbsp;Xia Li,&nbsp;Fen Li,&nbsp;Shaoying Wu","doi":"10.1002/arch.22141","DOIUrl":"10.1002/arch.22141","url":null,"abstract":"<p><i>Megalurothrips usitatus</i> Bagnall, an important pest of bean plants, is primarily managed with synthetic insecticides. <i>M. usitatus</i> has developed considerable resistance to various insecticides in multiple cowpea-growing areas in Hainan Province, China, posing challenges to its control in the field. Light control technology is a potentially effective physical control method for <i>M. usitatus</i>. The vision of thrips is highly sensitive to UV light, whereas other biological characteristics remain unknown. Therefore, this study evaluated the effects of ultraviolet light on the biological characteristics of <i>M. usitatus</i>. Results showed that the egg, larval, and pupal stages of <i>M. usitatus</i> were significantly shortened, and the emergence rate (79.59%) and adult survival rate (77.95%) were reduced under a devoid of UV light environment (UV−), compared with the full-spectrum light (control treatment group, CK) (<i>p</i> &lt; 0.05). However, the single spawning quantity and total amount of spawning were significantly higher, and the sex ratio (57%) was the highest under UV− (<i>p</i> &lt; 0.05). Single UV light (UV+) only affected the pupation rate. Also, the antioxidant enzymes, polyphenol oxidase, superoxide dismutase (SOD), and peroxidase activities were significantly and negatively correlated with the progression of generations under UV−, whereas catalase and SOD activities were significantly and positively correlated with the progression of generations under UV+. The UV− light conditions significantly interfered with the behavior selection of <i>M. usitatus</i>. The results of this study showed that the adaptability of <i>M. usitatus</i> populations would be greatly reduced in the absence of ultraviolet light, providing a theoretical basis for the control of <i>M. usitatus</i> populations.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the temperature adaptation of three rubber tree pest mites based on their two-sex life table","authors":"Weihua Wang,&nbsp;Sanlian Wan,&nbsp;Lijiu Zheng,&nbsp;Fangping Zhang,&nbsp;Junyu Chen","doi":"10.1002/arch.22138","DOIUrl":"10.1002/arch.22138","url":null,"abstract":"<p>To determine the optimal temperature range for the development and reproduction of three spider mites (<i>Eotetranychus sexmaculatus</i>, <i>Eotetranychus orientalis</i>, and <i>Oligonychus biharensis</i>), this study investigated their developmental period, survival rate, lifespan, and reproduction under five temperatures, 21, 24, 27, 30, and 33°C, to predict and control in the field. With the gathered data, a two-sex life table was constructed for each of them. The results revealed that as the temperature increased, both <i>O. biharensis</i> and <i>E. orientalis</i> displayed a gradual reduction in their generation period. Furthermore, an inverse relationship was observed between lifespan and temperature for all three spider mite species. When examining the survival rates at varying temperatures, <i>E. sexmaculatus</i> exhibited the highest rate (98%) at 33°C, while <i>E. orientalis</i> and <i>O. biharensis</i> demonstrated their highest survival rates at 24°C, reaching 90% and 100% respectively. Regarding reproduction, <i>O. biharensis</i> displayed the highest oviposition rates at 30°C with an average of 17.45 eggs per individual. Conversely, <i>E. sexmaculatus</i> and <i>E. orientalis</i> exhibited the highest oviposition rates at 33°C, averaging at 15.22 and 21.38 eggs per individual respectively. Significantly higher intrinsic growth rates were observed for <i>O. biharensis</i> and <i>E. orientalis</i> at 33°C, with rates of 0.22 and 0.26 respectively. In contrast, <i>E. sexmaculatus</i> demonstrated the highest intrinsic growth rate at 27°C. The temperature of 27°C was more suitable for the growth of the <i>E. sexmaculatus</i>, while 33°C was the optimal temperature for the <i>E. orientalis</i> and <i>O. biharensis</i>. The current findings provide valuable guidance for the control and prevention of these three spider mites.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological function of Bombyx Toll9-2 in the silkworm (Bombyx mori) larval midgut: Activation by Escherichia coli/lipopolysaccharide and regulation of growth","authors":"Jisheng Liu,&nbsp;Weifeng Yang,&nbsp;Wenli Liao,&nbsp;Yanling Huang,&nbsp;Weijian Chen,&nbsp;Xiaoling Bu,&nbsp;Shiyi Huang,&nbsp;Wanyi Jiang,&nbsp;Luc Swevers","doi":"10.1002/arch.22130","DOIUrl":"10.1002/arch.22130","url":null,"abstract":"<p>Toll receptors are important regulators of insects' innate immune system which, upon binding of pathogen molecules, activate a conserved signal transduction cascade known as the Toll pathway. RNA interference (RNAi) is a powerful tool to study the function of genes via reverse genetics. However, due to the reported refractory of RNAi efficiency in lepidopteran insects, successful reports of silencing of Toll receptors in the silkworm <i>Bombyx mori</i> have not been reported yet. In this study, a Toll receptor of the silkworm <i>Bombyx Toll9-2</i> (<i>BmToll9-2</i>) was cloned and its expression and function were analyzed. The results showed that <i>BmToll9-2</i> contains an ectodomain (ECD) with a signal peptide and nine leucine-rich repeats, a transmembrane helix, and a cytoplasmic region with a Toll/interleukin-1 domain. Phylogenetic analysis indicates that BmToll9-2 clusters with other insect Toll9 receptors and mammalian Toll-like receptor 4. Oral infection of exogenous pathogens showed that the Gram-negative bacterium <i>Escherichia coli</i> and its main cell wall component lipopolysaccharide (LPS), as well as the Gram-positive bacterium <i>Staphylococcus aureus</i> and its main cell wall component peptidoglycan, significantly induce <i>BmToll9-2</i> expression in vivo. LPS also induced the expression of <i>BmToll9-2</i> in BmN4 cells in vitro. These observations indicate its role as a sensor in the innate immunity to exogenous pathogens and as a pathogen-associated receptor that is responsive to LPS. RNAi of <i>BmToll9-2</i> was effective in the midgut and epidermis. RNAi-mediated knock-down of <i>BmToll9-2</i> reduced the weight and growth of the silkworm. Bacterial challenge following RNAi upregulated the expression of <i>BmToll9-2</i> and rescued the weight differences of the silkworm, which may be related to its participation in the immune response and the regulation of the microbiota in the midgut lumen of the silkworm larvae.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honeybee (Apis mellifera L.) pollination enhances the yield and flavor quality of kiwifruit","authors":"Quanzhi Ji,&nbsp;Xue Wang,&nbsp;Ting Huang,&nbsp;Xinyu Wang,&nbsp;Yazhou Zhao","doi":"10.1002/arch.22139","DOIUrl":"10.1002/arch.22139","url":null,"abstract":"<p>Pollination is essential for achieving high yields and enhancing the quality of kiwifruit cultivation, both of which significantly influence growers' interests and consumers' preferences. However, compared to studies on yield, there are fewer studies exploring the impact of pollination methods on the flavor of kiwifruit <i>Actinidia chinensis</i> Planchon. This study examined the effects of bee (<i>Apis mellifera</i> L.) pollination and artificial pollination on the yield and flavor of kiwifruit in the main producing areas of China. Compared with those pollinated artificially, bee-pollinated kiwifruit exhibited a greater fruit set rate, heavier fruit weight, and greater number of seeds. Notably, the number of seeds was positively correlated with fruit weight in bee-pollinated kiwifruit, whereas no such correlation was detected in artificially pollinated fruit. Bee pollination not only enhanced the yield but also improved the flavor of kiwifruit. Specifically, bee-pollinated kiwifruit contained higher levels of sucrose and lower concentrations of glucose and fructose, while the acid content was less affected by pollination methods. Furthermore, significant differences were observed in the volatile organic compound (VOC) levels in kiwifruit subjected to different pollination treatments, with bee-pollinated fruit exhibiting a superior flavor. Our findings provide new insights into the beneficial role of bee pollination in enhancing kiwifruit yield and quality, underscoring the crucial importance of bees in kiwifruit pollination.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"116 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}