Archives of Insect Biochemistry and Physiology最新文献

{"title":"Master Factors of Insect Metamorphosis; Chinmo, Kr-h1, BR-C, E93.","authors":"Hideki Kawasaki","doi":"10.1002/arch.70169","DOIUrl":"10.1002/arch.70169","url":null,"abstract":"<p><p>Kr-h1, BR-C and E93 are called Master factors of insect metamorphosis. Moreover, recently identified factor, Chinmo is included here. These factors are discussed from several stand points here. Insect evolved from ametabola through hemimetabola to holometabola. Holometabolous insect shows metamorphosis through the insect hormones, and JH or ecdysone regulates four factors; Chinmo, Kr-h1, BR-C and E93, punctuating larval, pupal and adult stages. Ecdysone was reported to upregulate CA activity, and JH was also reported to maintain the secretory activity of PG in holometabolous insect. Thus, JH and ecdysone interact each other until penultimate larval instar of holometabolous insect. Both hormones almost disappear at the early stage of the final larval instar, then low concentrations of ecdysteroid begins to be secreted and BR-C is activated, which has been explained to bring the commitment to the pupal stage. Now, by the identification of Chinmo, explanation of pupal commitment should be changed. Chinmo and Kr-h1 repress BR-C and E93 expression. E93 also represses BR-C expression, thus larval, pupal and adult stages are controlled through the interaction of factors. E93 is expressed in embryo of ametabolous and hemimetabolous insect, which is suggested to bring nymphal form, but is not expressed in embryo of holometabolous insect. Chromatin accessibility by Chinmo and E93, and the relation of these factors with insect evolution is to be elucidated.</p>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"122 1","pages":"e70169"},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148090050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148087748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148085595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome Simplification During Metamorphosis in Larva and Adults of Armigeres subalbatus (Coquillett, 1898) (Culicidae) Revealed by Shotgun Metagenomics","authors":"Manash Pratim Sarmah,&nbsp;K. Zoramthara,&nbsp;Roselyn Manngaihsiam,&nbsp;Hathorkhi Hala Boro,&nbsp;Abdelgani Gumaa Abdelmuala Baraka,&nbsp;Ahmed-Laskar Saeed,&nbsp;Guruswami Gurusubramanian,&nbsp;Kiran R. Kharat","doi":"10.1002/arch.70159","DOIUrl":"10.1002/arch.70159","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Armigeres subalbatus</i> is medically significant vector for filarial worms and the Japanese encephalitis virus. Shotgun metagenomic sequencing was employed to investigate the bacterial communities in <i>A. subalbatus</i> mosquitoes. The diversity metrics (Shannon H′, Simpson 1-D, Berger-Parker) were calculated for larval and adult stages. De novo assembly and binning were used to recover metagenome-assembled genomes (MAGs) with &gt; 82% completeness and &lt; 4% contamination. Functional profiling assessed gene expression via transcripts per million (TPM) and clusters of orthologous groups (COG) categories. Larval microbiomes showed high alpha diversity (Shannon H′ ≈ 1.336 ± 0.163, Simpson 1-D = 0.684 ± 0.046), dominated by Gammaproteobacteria (<i>Aeromonas</i>, <i>Morganella,</i> and <i>Yersinia</i>) and Bacteroidota, with persistent Shewanella and Acinetobacter. Adult microbiomes exhibited low diversity (Shannon H′ = 0.637 ± 0.100, Berger-Parker = 0.682 ± 0.026), near-monoculture dominated by <i>Aeromonas hydrophila</i>, alongside low-abundance <i>Stenotrophomonas</i>, <i>Pseudomonas</i>, and <i>Microbacterium</i>. Six high-quality MAGs were recovered: larval (<i>Bacteroidota</i>, <i>Shewanella,</i> and <i>Acinetobacter</i>); adult (<i>Acinetobacter</i>, <i>Stenotrophomonas,</i> and <i>Shewanella</i>), confirming persistence of Shewanella and Acinetobacter, absence of Bacteroidota, and emergence of Stenotrophomonas in adults. Adult microbiomes displayed metabolic hyperactivity, with 1.5–4 times higher transcriptional output across COG categories compared to larvae. Chemotaxis [Methyl-accepting chemotaxis protein (MCP), K03406: ~6000 TPM in adults vs. &lt; 1000 TPM in larvae] and ABC transporters (PF00005: &gt; 10,000 TPM in adults) dominated adults, while larval expression was balanced among housekeeping functions. The microbiome undergoes significant restructuring during mosquito development, shifting from diverse larval communities to metabolically active, low-diversity adult assemblages. Recovered MAGs provide a genomic basis for future studies on mosquito microbiota dynamics and functions.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147687397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148077849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC-MS Based Study of Haemolymph Metabolomics in Male Spodoptera mauritia Boisduval, on Post-Treatment of Flufenoxuron","authors":"V. Reshma,&nbsp;E. M. Manogem","doi":"10.1002/arch.70157","DOIUrl":"10.1002/arch.70157","url":null,"abstract":"<div>\u0000 \u0000 <p>Flufenoxuron exhibits excellent chitin-inhibiting activity against several insect species, including Lepidoptera. The disruption of the chitin synthesis process can have a significantly negative impact on several developmental systems of insects. However, current knowledge of the metabolomic effect on <i>Spodoptera mauritia</i> larvae remains underdeveloped. This study employed Gas Chromatography–Mass Spectrometry (GC-MS)-based untargeted metabolomics to investigate the changes in metabolic activity and biochemical effects of flufenoxuron in <i>S. mauritia</i> haemolymph, thereby illustrating the possible mode of action. Metabolomic analysis of haemolymph was conducted on exposure to two different sublethal concentrations of flufenoxuron (0.00068 and 0.00015 µg) in the sixth instar stage of <i>S. mauritia</i>. The effect of flufenoxuron was investigated on selected differently abundant metabolites in GC-MS-based metabolomics results. Notably, carbohydrate and fatty acid levels were significantly reduced, indicating severe nutritional and energetic stress. Pathway analysis indicated that carbohydrate, lipid and amino acid metabolism are strongly associated with the effects of the chitin synthesis inhibitor. The findings substantiate the flufenoxuron-mediated disturbance in metabolites and metabolic pathways. The findings of the study deepen our understanding of the physiological disruptions caused by flufenoxuron, highlighting its potential hazards in agricultural applications against <i>S. mauritia</i>.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insect Laccase Like Multi Copper Oxidases: Enzymatic Functions and Applications","authors":"Priscila G. Polo,&nbsp;José Galián","doi":"10.1002/arch.70155","DOIUrl":"10.1002/arch.70155","url":null,"abstract":"<div>\u0000 \u0000 <p>Laccases are multicopper oxidases (MCOs) widely distributed across plants, fungi, bacteria, and animals, where they catalyze the oxidation of aromatic and non-aromatic substrates coupled to the four-electron reduction of molecular oxygen to water. Although microbial laccases have been extensively exploited for bioremediation and industrial catalysis, insect laccases represent a comparatively understudied but functionally diverse group with key roles in physiology, development, and ecological adaptation. Insects typically encode at least two highly conserved laccases, laccase 1 (MCO1) and laccase 2 (MCO2), along with additional paralogs in specific lineages. These enzymes participate in the detoxification of plant allelochemicals, cuticle sclerotization and pigmentation, immune responses, iron homeostasis, reproductive processes, and interactions with symbiotic microorganisms. Here, we provide a comprehensive synthesis of the molecular diversity, evolutionary implications, and physiological functions of insect laccases. We also highlight emerging biotechnological applications, including their contributions to lignocellulose processing, the degradation of xenobiotics, and the oxidative priming of synthetic polymers within insect microbiota. Finally, we outline future research directions aimed at harnessing insect laccases and their microbial counterparts as alternative oxidative biocatalysts for sustainable biotechnology.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148075447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"121 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148075448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}