一种具有广泛昆虫致病谱的线虫菌株 Oscheius Tipulae 及其在控制双翅目害虫中的应用

IF 1.5 4区 农林科学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Eticha Abdisa, Mojtaba Esmaeily, Jiyoon Kwon, Gahyeon Jin, Yonggyun Kim
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引用次数: 0

摘要

从土壤样本中分离出一种昆虫病原线虫 Oscheius tipulae。形态学和分子标记支持了该物种的鉴定。线虫分离物对不同的目标昆虫(包括鳞翅目、鞘翅目和双翅目昆虫)具有致病性。该线虫的致病力与著名的昆虫病原线虫 Steinernema carpocapsae 对相同目标昆虫的致病力相似。对这两种线虫进行的元基因组学比较分析预测,它们的肠道中总共存在 272 种细菌,其中 51 种细菌是这两种线虫共有的。特别是,共同的肠道细菌包括几种昆虫病原菌,其中包括 Xenorhabdus nematophila,它是已知的鲤科线虫的共生细菌。添加地塞米松会增强 O. tipulae 对昆虫的线虫毒力,但添加花生四烯酸则会抑制这种毒力,这表明目标昆虫对线虫感染的免疫防御是由二十烷酸介导的,而二十烷酸会受到线虫共生细菌的控制。与 S. carpocapsae 不同,O. tipulae 对包括果蝇、葱蝇和蚊子在内的双翅目昆虫具有很强的毒力。在盆栽和田间试验中,O. tipulae 对威尔士洋葱根瘤中的洋葱蛆(Delia platura)具有特别高的防治效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Nematode Isolate, Oscheius Tipulae, Exhibiting a Wide Entomopathogenic Spectrum and its Application to Control Dipteran Insect Pests

An entomopathogenic nematode, Oscheius tipulae, was isolated from a soil sample. The identification of this species was supported by morphological and molecular markers. The nematode isolate exhibited pathogenicity against different target insects including lepidopteran, coleopteran, and dipteran insects. The virulence of this nematode was similar to that of a well-known entomopathogenic nematode, Steinernema carpocapsae, against the same insect targets. A comparative metagenomics analysis of these two nematode species predicted the existence of a combined total of 272 bacterial species in their intestines, of which 51 bacterial species were shared between the two nematode species. In particular, the common gut bacteria included several entomopathogenic bacteria including Xenorhabdus nematophila, which is known as a symbiotic bacterium to S. carpocapsae. The nematode virulence of O. tipulae to insects was enhanced by an addition of dexamethasone but suppressed by an addition of arachidonic acid, suggesting that the immune defenses of the target insects against the nematode infection is mediated by eicosanoids, which would be manipulated by the symbiotic bacteria of the nematode. Unlike S. carpocapsae, O. tipulae showed high virulence against dipteran insects including fruit flies, onion flies, and mosquitoes. O. tipulae showed particularly high control efficacies against the onion maggot, Delia platura, infesting the Welsh onion in the rhizosphere in both pot and field assays.

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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
115
审稿时长
12 months
期刊介绍: Archives of Insect Biochemistry and Physiology is an international journal that publishes articles in English that are of interest to insect biochemists and physiologists. Generally these articles will be in, or related to, one of the following subject areas: Behavior, Bioinformatics, Carbohydrates, Cell Line Development, Cell Signalling, Development, Drug Discovery, Endocrinology, Enzymes, Lipids, Molecular Biology, Neurobiology, Nucleic Acids, Nutrition, Peptides, Pharmacology, Pollinators, Proteins, Toxicology. Archives will publish only original articles. Articles that are confirmatory in nature or deal with analytical methods previously described will not be accepted.
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