Archives of Insect Biochemistry and Physiology最新文献

{"title":"Glutathione S-Transferase Contributes to the Resistance of Megalurothrips usitatus Against Lambda-Cyhalothrin by Strengthening Its Antioxidant Defense Mechanisms","authors":"Wenbo Dong,&nbsp;Chaozheng Wang,&nbsp;Xia Li,&nbsp;Tianbao Huang,&nbsp;Fen Li,&nbsp;Shaoying Wu","doi":"10.1002/arch.70010","DOIUrl":"https://doi.org/10.1002/arch.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>The damage caused by <i>Megalurothrips usitatus</i>, a common pest, has significantly affected the Chinese vegetable industry. The inappropriate application of chemical pesticides has caused <i>M. usitatus</i> to become highly resistant to conventional insecticides. Glutathione S-transferase (GST), known for its multifunctional properties, contributes to detoxification and antioxidation. It enhances insects' adaptability to pesticides by facilitating the elimination of lipid peroxidation products resulting from pyrethroid insecticides. This research employed RT-qPCR to identify GST genes that exhibited significant expression in response to lambda-cyhalothrin stress. It also quantified changes in antioxidant and apoptosis markers within the <i>M. usitatus</i> under lambda-cyhalothrin exposure. The functional significance of GST was validated by assessing alterations in the antioxidant defense system and resistance to lambda-cyhalothrin following the inhibition of GST activity. The study's outcomes indicated that <i>MuGSTs1</i> was markedly upregulated in response to lambda-cyhalothrin stress (<i>p</i> &lt; 0.0001). The GST activity was effectively suppressed by the specific inhibitor, diethyl maleate, achieving an inhibition rate of 64.05%. Following the inhibition of GST, the overall antioxidant capacity was reduced by 3.1-fold compared with the control, and the <i>M. usitatus</i> exhibited a 7.91-fold increase in sensitivity to lambda-cyhalothrin. These findings confirm the pivotal role of GST in the oxidative stress response of the <i>M. usitatus</i> and their contribution to the development of resistance to lambda-cyhalothrin through enhanced antioxidant defenses. This research offers valuable perspectives on the adaptive reactions of insects to chemical stressors, facilitating the management of resistance and the formulation of effective pest control strategies.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Function of PxGrp78 in the Reproduction of Plutella xylostella","authors":"Si-Yuan Gong,&nbsp;Can-Xin Zhang,&nbsp;Chen-Meng Xue,&nbsp;Xiao-Han Yang,&nbsp;Chao-Bin Xue","doi":"10.1002/arch.70007","DOIUrl":"https://doi.org/10.1002/arch.70007","url":null,"abstract":"<div>\u0000 \u0000 <p>Glucose-regulated protein 78 (Grp78), a crucial molecular chaperone in the endoplasmic reticulum, has been extensively investigated in vertebrates. However, its functional exploration in insects remains limited. This study cloned the full-length cDNA sequence of <i>Grp78</i> in <i>Plutella xylostella</i> (L.), which is 2583 bp long. The open reading frame (ORF) is 2004 bp in length and encodes a total of 667 amino acids, including three conserved characteristic sequences of the HSP70 family. <i>PxGrp78</i> is expressed in various developmental stages of <i>P. xylostella</i>, with the highest expression observed in third instar larvae and higher expression in female adults compared to male adults. The interference with <i>PxGrp78</i> in female adults was found to significantly reduce the quantity of egg laying and the hatching rate, as well as shorten the oviposition period and down-regulate the expression of the <i>PxVg</i> gene. These results suggested an important role for <i>PxGrp78</i> in the reproduction of <i>P. xylostella</i>.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Aralkylamine N-Acetyltransferase in the Response to Antioxidative Stress in the Fruit Fly Drosophila Melanogaster Adults","authors":"Arnau Rodríguez-Illamola,&nbsp;Roman Sidorov,&nbsp;Radmila Čapková-Frydrychová,&nbsp;Dalibor Kodrík","doi":"10.1002/arch.70009","DOIUrl":"https://doi.org/10.1002/arch.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>In multicellular organisms, the indole melatonin synthesized by aralkylamine N-acetyltransferase (AANATI) serves as an antioxidant. To test this, sex-mixed 3-day-old mated fly adults <i>bw</i>^<i>1</i> and AANAT1 homozygous recessive loss-of-function mutant (<i>bw AANAT1</i>^<i>lo</i>) of <i>Drosophila melanogaster</i> were fed by a standard diet or by one containing paraquat (PQ, 1,1′-dimethyl-4,4′-bipyridilium dichloride hydrate) at a final concentration of 15.5 mM. Experiment lasted 8 h and began at 11 a.m. In <i>bw</i>^<i>1</i> flies the paraquat treatment resulted in a significant (evaluated by Student's <i>t</i>-tests) decrease of the superoxide dismutase (SOD) activity and an increase the catalase (CAT) and glutathione S-transferase (GST) activities. Meanwhile, in these flies, total Antioxidative capacity (TAC) was significantly curbed by the paraquat presence. Importantly, these changes were not observed in the AANAT1-mutants. Thus, melatonin seems to play an important defence role against the oxidative stress elicited by paraquat.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Structure and Function of Intestinal Microorganisms in Silkworm Maggot Exorista sorbillans","authors":"Siyin Zhong,&nbsp;Zhe Jiang,&nbsp;Jiabao Zhang,&nbsp;Zhiya Gu,&nbsp;Jing Wei,&nbsp;Bing Li,&nbsp;Fanchi Li","doi":"10.1002/arch.70008","DOIUrl":"10.1002/arch.70008","url":null,"abstract":"<div>\u0000 \u0000 <p>Insects have important symbiotic relationships with their intestinal microbiota. The intestinal microbiota is involved in or influences various processes in insects such as development, metabolism, immunity, and reproduction. Currently, research on the intestinal microbiota of parasitic insects is still in its early stages. The tachinid parasitoid <i>Exorista sorbillans</i> is a dipteran parasitic insect, with the silkworm (<i>Bombyx mori</i>) being its main host. Silkworms parasitized by <i>E. sorbillans</i> can suffer from severe silkworm maggot disease, which also poses a serious threat to sericulture. In this study, the intestinal microbiota of larval <i>E. sorbillans</i> at three instar stages was analyzed using 16S rRNA amplicon sequencing to explore the community composition of the intestinal microbiota. Additionally, using conventional culture methods, six cultivable strains were isolated and identified from the larval <i>E. sorbillans</i> on an antibiotic-free LB medium, and four cultivable strains were isolated and identified from the hemolymph of parasitized silkworms. This study investigated the <i>E. sorbillans</i> from the perspective of intestinal microbiota, elucidating the composition and structural characteristics of the intestinal microbiota of the tachinid parasitoid, and preliminarily discussing the functional roles of several major microorganisms, which helps to further clarify the potential mechanisms of interaction between the parasitoid and the silkworm.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PGE2 Binding Affinity of Hemocyte Membrane Preparations of Manduca sexta and Identification of the Receptor-Associated G Proteins in Two Lepidopteran Species","authors":"Falguni Khan,&nbsp;Hasan Tunaz,&nbsp;Eric Haas,&nbsp;Yonggyun Kim,&nbsp;David Stanley","doi":"10.1002/arch.70005","DOIUrl":"10.1002/arch.70005","url":null,"abstract":"<div>\u0000 \u0000 <p>Prostaglandin E₂ (PGE₂) is an eicosanoid that mediates a range of physiological actions in vertebrates and invertebrates, including reproduction and immunity. The PGE₂ receptor was identified and functionally assessed in two lepidopteran insects, <i>Manduca sexta</i> and <i>Spodoptera exigua</i>. However, its binding affinity to the receptor has not been reported. The PGE₂ receptor is a G-protein coupled receptor (GPCR) although its corresponding G-protein is not identified. PGE₂ binding assays were performed with membrane preparations from hemocytes of <i>M. sexta</i> larvae. We recorded an optimal binding in 4 h reactions conducted at pH 7.5 with 12 nM tritium-labeled PGE₂. We found that hemocytes express a single population of PGE₂ binding sites with a high affinity (Kd = 35 pmol/mg protein), which are specific and saturable. The outcomes of experiments on the influence of purine nucleotides suggested these are functional GPCRs. A bioinformatics analysis led to a proposed trimeric G-protein in the <i>S. exigua</i> transcriptome, in which the Gα subunit is classified into five different types: Gα(o), Gα(q), Gα(s), Gα(12), and Gα(f). After confirming expressions of these five types in <i>S. exigua</i>, individual RNA interference (RNAi) treatments were applied to the larvae using gene-specific double-stranded RNAs. RNAi treatments specific to Gα(s) or Gα(12) gene expression significantly suppressed the cellular immune responses although the RNAi treatments specific to other three Gα components did not. While PGE₂ treatments led to elevated hemocyte cAMP or Ca²⁺ levels, the RNAi treatments specific to Gα(s) or Gα(12) genes led to significantly reduced second messenger levels under PGE_2, although the RNAi treatments specific to the other three Gα components did not. These results showed that the PGE₂ receptor has high PGE₂ affinity in the nanomolar range and binds G-proteins containing a Gα(s) or Gα(12) trimeric component in <i>S. exigua</i> and <i>M. sexta</i>, and likely, all lepidopteran insects.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolism of Furanocoumarins by Three Recombinant CYP9A Proteins From the Polyphagous Cotton Bollworm Helicoverpa armigera","authors":"Kai Tian,&nbsp;Jiang Zhu,&nbsp;Xinghui Qiu","doi":"10.1002/arch.70004","DOIUrl":"10.1002/arch.70004","url":null,"abstract":"<div>\u0000 \u0000 <p>Furanocoumarins are a class of chemical compounds with phototoxic properties. For herbivores, efficient detoxification of such defense compounds is the prerequisite to feed successfully on furanocoumarin-containing plants. The cotton bollworm <i>Helicoverpa armigera</i> is a very important polyphagous pest in agriculture, but how it copes with toxic furanocoumarins in some of its host plants is not well understood. Given that cytochrome P450s are well known for their capacity in xenobiotic metabolism, this study attempted to explore the potential roles of cytochrome P450s in furanocoumarin transformation in this pest. Our data showed that two linear structures (psoralen and xanthotoxin) could be metabolized by three recombinant CYP9A enzymes, but no detectable depletion was observed for the linear one with the 8-dimethylallyloxy substituent on the coumarin moiety (imperatorin) and the angular furanocoumarin (angelicin). Initial epoxidation of the double bond connecting C2' and C3' of the furano ring following by cleavage of the epoxidated furan ring, leading to the formation of more soluble, less reactive and nonphotosensitizing metabolites, was identified as a common mechanism of linear furanocoumarin metabolism using a quadrupole/time-of-flight (Q-TOF) mass spectrometry interfaced with a high performance liquid chromatography (HPLC) system. Our data demonstrated that multiple P450s were involved in the detoxification of linear furanocoumarins in the cotton bollworm. These findings contribute to a better understanding of the biochemical basis of adaptation to plant defense chemicals in this economically important pest.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α-Solanine and α-Tomatine Affect the Retrocerebral Complexes of Tenebrio molitor and Zophobas atratus Beetles","authors":"Zbigniew Adamski,&nbsp;Polyxeni Nikolaou,&nbsp;Pawel Marciniak","doi":"10.1002/arch.70003","DOIUrl":"10.1002/arch.70003","url":null,"abstract":"<div>\u0000 \u0000 <p>Presently, the European Commission is moving forward with a plan to reduce the use of chemical pesticides and increase the percentage of organic farming by 2030; this plan demands increased availability of biopesticides for organic farming. Glycoalkaloids are a class of molecules found in certain plants, including tomatoes and potatoes, which contain sugar and an alkaloid group. These compounds are known to have insecticidal properties, as they can act as natural defenses against insect pests. This study examined how a biological substance, glycoalkaloids, affects mortality and the retrocerebral complex of two beetle species, <i>Tenebrio molitor</i> and <i>Zophobas atratus</i>. More specifically, we focused on two glycoalkaloids, α-solanine and α-tomatine. Although these two insects belong to the same taxonomic family, they were not equally susceptible to these substances. In the mortality test, we observed that the <i>T. molitor</i> beetle seemed more susceptible, whereas the changes in the retrocerebral complex were more significant in the case of <i>Z. atratus</i>. In general, the effects vary between substances and insects, and changes in the retrocerebral complex may impair the reproduction of insects.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kaempferol Extends Male Lifespan Under Blue Light Irradiation in Drosophila","authors":"Qimeng He,&nbsp;Hui Jin,&nbsp;Jie Shen","doi":"10.1002/arch.70001","DOIUrl":"10.1002/arch.70001","url":null,"abstract":"<div>\u0000 \u0000 <p>Short-wavelength blue light is ubiquitous in daily life and has a lasting destructive influence. Its potential harm to biological health is significant. This study used <i>Drosophila</i> as a model organism to investigate the protective effects of kaempferol, a flavonoid, against the toxicity of blue light. It also examined its physiological effects on <i>Drosophila</i> under blue light irradiation. In this experiment, fruit flies were fed with three different concentrations of kaempferol solutions (0.1, 0.01, and 0.001 mol/L) dissolved in food. The survival rate and physiological indexes of <i>Drosophila</i> were investigated under blue light irradiation of 2500 lux. The results showed that 0.1 mol/L kaempferol increased the activity of male flies during the day and significantly extended the male survival time under blue light irradiation. However, the study found that kaempferol did not significantly prolong the survival time of <i>Drosophila</i> in the oxidative stress experiment, and no significant difference was observed in the feeding experiment. In summary, our research found that kaempferol, at the concentration of 0.1 mol/L, has a protective effect on <i>Drosophila</i> under blue light irradiation, potentially achieved through alterations in circadian rhythm.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Adaptations in Phosphine-Resistant Tribolium castaneum Driven by Mitochondrial Enzyme Variability and Gene Expression","authors":"Mudagadde G. Deeksha,&nbsp;Suresh M. Nebapure,&nbsp;Anil Dahuja,&nbsp;Doddachowdappa Sagar,&nbsp;Ramcharan Bhattacharya,&nbsp;Sabtharishi Subramanian","doi":"10.1002/arch.70002","DOIUrl":"10.1002/arch.70002","url":null,"abstract":"<div>\u0000 \u0000 <p>Phosphine fumigation is essential for controlling storage pests like <i>Tribolium castaneum</i>, but its frequent application has resulted in resistance, primarily due to mutations in the Dihydrolipoamide dehydrogenase (DLD) gene associated with the <i>rph2</i> allele. This study demonstrates that the Patiala population exhibits homozygous resistance variations across populations, contrasting with the susceptibility observed in laboratory cultures. Our assessment of mitochondrial DLD and Cytochrome c oxidase (COX) activities showed significantly elevated levels in the Patiala population, with increases of approximately sevenfold for DLD and 6.92-fold for COX, indicating mitochondrial adaptations for enhanced energy production. Kinetic analyses of DLD in the resistant Patiala population showed a higher V_max (0.005 mmol/min) and a significantly increased Km (16.66 mM), indicating variations in maximal enzyme activity and substrate affinity. Furthermore, resistant <i>T. castaneum</i> populations displayed substantial upregulation of DLD and COX gene expression, with DLD expression increasing by 10.53-fold and COX expression peaking at 102.57-fold in Patiala. Pearson correlation analysis indicated strong positive correlations (<i>r</i> &gt; 0.8) between enzymatic activity and gene expression for both DLD and COX, suggesting a coordinated role in resistance mechanisms. The PCA biplot illustrated distribution patterns of enzymatic activity and gene expression among field-resistant populations, highlighting the association between increased resistance and elevated enzymatic activity and gene expression levels. Therefore, the upregulation of DLD and COX activities in resistant populations underscores their critical roles in counteracting phosphine, reflecting metabolic reprogramming for improved energy production under stress.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Aphid Pest Superclone Benefits From a Facultative Bacterial Endosymbiont in a Host-Dependent Manner, Leading to Reproductive and Proteomic Changes","authors":"Leandro Mahieu,&nbsp;Angélica González-González,&nbsp;María Eugenia Rubio-Meléndez,&nbsp;Mario Moya-Hernández,&nbsp;Frederic Francis,&nbsp;Claudio C. Ramírez","doi":"10.1002/arch.22154","DOIUrl":"https://doi.org/10.1002/arch.22154","url":null,"abstract":"<div>\u0000 \u0000 <p>The English grain aphid, <i>Sitobion avenae</i>, is a significant agricultural pest affecting wheat, barley, and oats. In Chile, the most prevalent and persistent clone (superclone) of <i>S. avenae</i> harbors the facultative endosymbiont bacterium <i>Regiella insecticola</i>. To determine the role of this bacterium in the reproductive success of this superclone, the presence of <i>R. insecticola</i> was manipulated to assess its impact on (1) the reproductive performance of this clone on two host plant species (wheat and barley), (2) the production of winged morphs, (3) changes in the insects' proteomic profiles, and (4) the root/shoot ratio of plant. It was found that the reproductive performance of this <i>S. avenae</i> superclone varied across host plants, depending on the presence of the facultative bacterial endosymbiont. Aphids infected with <i>R. insecticola</i> showed higher reproductive success on wheat, while the opposite effect was observed on barley. Aphid biomass was greater when infected with <i>R. insecticola</i>, particularly on barley. Additionally, aphids harboring <i>R. insecticola</i> exhibited a higher proportion of winged individuals on both host plants. Protein regulation in aphids on wheat was lower compared to those on barley. A higher root/shoot biomass ratio was observed in wheat plants compared to barley when infested by <i>R. insecticola</i>-infected aphid. Thus, <i>R. insecticola</i> significantly influences the reproductive performance and proteomic profile of a <i>S. avenae</i> superclone, with these effects shaped by the host plant. This suggests that the interaction between the host plant and the facultative endosymbiont contributes to the ecological success of this superclone.</p></div>","PeriodicalId":8281,"journal":{"name":"Archives of Insect Biochemistry and Physiology","volume":"117 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}