Design and Synthesis of Nano-dsRNA Nanoparticles Targeting Bombyx mori Acetyltransferase BmCBP and Its Impact on BmApoLp-III Expression

Abstract

As a key acetyltransferase, Bombyx mori cAMP response element-binding protein (CREB)-binding protein (BmCBP) plays an important role in post-translational modifications, affecting protein stability and immune response in silkworm; however, its mechanism of action remains unclear. In this study, we designed and synthesized Nano-dsRNA nanoparticles targeting BmCBP and validated their impact on BmApoLp-III protein expression. Using an E. coli expression system, we successfully synthesized nano-dsRNA targeting BmCBP, which significantly reduced BmCBP transcription level, thereby decreasing the acetylation and protein expression levels of BmApoLp-III. The knock-down of BmCBP by nano-dsRNA also increased the ubiquitination level of BmApoLp-III, suggesting a competitive relationship between acetylation and ubiquitination. These findings show that design and synthesis of efficient nano-dsRNA nanoparticles targeting BmCBP provide a novel tool and approach for studying gene expression and regulation in silkworm and other insects.