RNAi-Mediated Knockdown of Tektins Does Not Affect Male Fertility in Lygus hesperus

IF 1.5 4区 农林科学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
J. Joe Hull, Kevin P. Le, Inana X. Schutze, Chan C. Heu, Roni J. Gross, Peter G. Fabrick, Joseph A. Rodriguez, Aiden M. Hull, Daniel Langhorst, Jeffrey A. Fabrick, Colin S. Brent
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引用次数: 0

Abstract

Mirid plant bugs (Hemiptera: Miridae), including Lygus hesperus (western tarnished plant bug), are key pests of numerous agricultural crops. While management of this pest relies heavily on chemical insecticides, the evolution of resistance and environmental concerns underscore the need for new and more effective approaches. Genetic-based strategies that target male fertiliy are currently being evaluated for population suppression. However, a lack of candidate gene targets with appropriate function, specifically in non-model species like L. hesperus, has hindered progress in the development and application of such approaches. Given their conserved role in stabilization of the flagella axoneme and association with sperm motility in many organisms, members of the tektin gene family represent logical targets for genetic-based sterilization. Here, we identified four homologs of the non-vertebrate tektin family from L. hesperus and used RNA interference-mediated knockdown to assess their roles in male fertility. Although transcription of the four tektins was predominantly in the testis, knockdown had negligible effects on either sperm abundance or male fertility. Our results suggest that tektins do not play a critical role in sperm fertilization of eggs in L. hesperus and are thus likely poor targets for genetic-based sterilization approaches in this species.

rnai介导的tektin蛋白敲低不影响雄性羊尾草的生育能力
斑蝽(半翅目:斑蝽科)是危害农作物的主要害虫,其中包括斑蝽(Lygus hesperus)。虽然这种害虫的管理严重依赖化学杀虫剂,但抗药性的演变和环境问题强调需要新的和更有效的方法。目前正在评估以男性生育为目标的基于基因的策略对人口抑制的作用。然而,缺乏具有适当功能的候选基因靶点,特别是在非模式物种如L. hesperus中,阻碍了这种方法的发展和应用。鉴于它们在许多生物体中稳定鞭毛轴突和与精子活力相关的保守作用,tektin基因家族成员代表了基于遗传的灭菌的逻辑目标。在这里,我们从L. hesperus中鉴定了非脊椎动物tektin家族的四个同源物,并使用RNA干扰介导的敲低来评估它们在雄性生殖能力中的作用。尽管这四种tektin的转录主要发生在睾丸中,但敲除对精子数量或男性生育能力的影响微不足道。我们的研究结果表明,tektinins在L. hesperus卵子的精子受精中没有发挥关键作用,因此可能是该物种基于遗传的绝育方法的不良目标。
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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
115
审稿时长
12 months
期刊介绍: Archives of Insect Biochemistry and Physiology is an international journal that publishes articles in English that are of interest to insect biochemists and physiologists. Generally these articles will be in, or related to, one of the following subject areas: Behavior, Bioinformatics, Carbohydrates, Cell Line Development, Cell Signalling, Development, Drug Discovery, Endocrinology, Enzymes, Lipids, Molecular Biology, Neurobiology, Nucleic Acids, Nutrition, Peptides, Pharmacology, Pollinators, Proteins, Toxicology. Archives will publish only original articles. Articles that are confirmatory in nature or deal with analytical methods previously described will not be accepted.
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