Aquaculture Research最新文献

{"title":"Green Boost for Nile Tilapia, Oreochromis niloticus: Unveiling the Multifaceted Effects of Chenopodium album Leaves Powder on Growth, Hematology, Antioxidant Defense, Nonspecific Immunity and Tolerance Against Aeromonas hydrophila","authors":"Javed Ahmed Ujan,&nbsp;Khalid Hussain Rind,&nbsp;Osman Sabri Kesbiç,&nbsp;Samrah Masud,&nbsp;Syed Sikandar Habib,&nbsp;Salim S. Al-Rejaie,&nbsp;Mohamed Mohany,&nbsp;Francesco Fazio","doi":"10.1155/are/8919050","DOIUrl":"https://doi.org/10.1155/are/8919050","url":null,"abstract":"<div>\u0000 <p>This study assessed the impact of dietary <i>Chenopodium album</i> leaf powder on various aspects of Nile tilapia (<i>Oreochromis niloticus</i>), including growth performance, hematological and biochemical profiles, antioxidant status, nonspecific immune response, and resistance to <i>Aeromonas hydrophila</i> infection. A 60-day feeding trial was conducted with 180 Nile tilapia fingerlings, with an initial average weight of 11.79 ± 0.23 g, fed diets supplemented with increasing levels of <i>C. album</i> at 0, 10, 20, and 30 g/kg. After feeding, the fish underwent an experimental challenge with the pathogen <i>A. hydrophila</i>. Following the challenge, the fish survival was monitored for 14 days. The results demonstrated that as the level of <i>C. album</i> supplementation increased, there were significant (<i>p</i>  &lt; 0.05) improvements in growth performance and a reduction in the feed conversion ratio (FCR). The hematological and biochemical profiles showed significant (<i>p</i>  &lt; 0.05) enhancements in the groups that received <i>C. album</i>-supplemented diets. Additionally, the antioxidant status in the fish serum significantly (<i>p</i>  &lt; 0.05) improved, as evidenced by increased activities of superoxide dismutase (SOD), catalase (CAT), and reduced glutathione. The nonspecific immune response, including lysozyme activity, phagocytic activity, and fish survival, also exhibited significant (<i>p</i>  &lt; 0.05) enhancements, particularly in the groups receiving 20–30 g/kg of <i>C. album</i>. Furthermore, there was a significant decrease in serum glucose and cortisol levels in the groups fed with 20–30 g/kg of <i>C. album</i>. In conclusion, <i>C. album</i> supplementation at 20–30 g/kg improved growth, immune response, and disease resistance in Nile tilapia. This diet also reduced stress markers, enhancing overall health.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8919050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synbiotic Supplementation Boosts Growth, Gut Health, and Immunity in Asian Fossil Catfish (Heteropneustes fossilis)","authors":"Sadia Salam Linda,&nbsp;Md. Jobayer Islam,&nbsp;Sadia Afrin Mou,&nbsp;Md. Hamidul Islam,&nbsp;Md. Shahjahan,&nbsp;M. Sadiqul Islam","doi":"10.1155/are/4542077","DOIUrl":"https://doi.org/10.1155/are/4542077","url":null,"abstract":"<div>\u0000 <p>This study explores the effects of synbiotics on growth, intestinal health, and immunity in the Asian fossil catfish (<i>Heteropneustes fossilis</i>), a crucial species in Southeast Asian aquaculture. With the rising use of antibiotics in aquaculture, which poses risks to both the environment and consumer health, there is an urgent need for sustainable alternatives. This study investigates how synbiotics—combinations of probiotics and prebiotics—can enhance fish health and performance. Four dietary treatments with varying synbiotic concentrations (0%, 4%, 6%, and 8%) were tested over 45 days. Results indicated that synbiotic supplementation significantly improved growth metrics, including final weight and specific growth rate (SGR), with the highest benefits observed at 8% inclusion. Additionally, dietary synbiotics enhanced intestinal health by increasing villi height and width and crypt depth and positively affected hematological parameters, notably elevating hemoglobin (Hb) levels. Histological analysis revealed improved liver tissue organization and hepatocyte morphology with higher synbiotic concentrations. These findings suggest that synbiotics offer substantial benefits for fish growth, health, and immunity, positioning them as a viable, ecofriendly alternative to antibiotics in aquaculture. Further research should aim to refine synbiotic formulations and investigate their underlying mechanisms to optimize their application in sustainable aquaculture practices.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4542077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Immune Responses Induced in Gibel Carp, Carassius auratus gibelio Immersion, and Intraperitoneal Vaccinated With Live Attenuated Cyprinid Herpesvirus 2 (CyHV-2)","authors":"Jia Wang,&nbsp;Weijie Zhang,&nbsp;Jizhuo Liu,&nbsp;Qiang Li,&nbsp;Zhitao Qi,&nbsp;Chang Wei","doi":"10.1155/are/5588289","DOIUrl":"https://doi.org/10.1155/are/5588289","url":null,"abstract":"<div>\u0000 <p>This study investigated the immune responses induced in gibel carp, <i>Carassius auratus gibelio</i>, following immersion and intraperitoneal (i.p.) vaccination with live attenuated Cyprinid herpesvirus 2 (CyHV-2). Transcriptome sequencing analysis was performed on the kidney tissues of the experimental fish at 3, 7, and 14 days postvaccination. A staggering 948 million readings were assembled into 96,891 genes, with an average length of 1214 bp. We analyzed the KEGG enrichment, differentially expressed genes (DEGs), and DEGs temporal expression patterns between the immersion and i.p. injection groups. Our findings revealed that both i.p. injection and immersion vaccination significantly altered immune-related genes in gibel carp, suggesting that the prepared attenuated vaccine effectively stimulates the fish’s immune response. Generally, the injection group exhibited higher gene expression levels in the kidney tissue earlier and to a greater extent. In contrast, the immersion group showed delayed immune responses with lower expression levels. The differential expression of 12 immune-related genes in immunized gibel carp, as detected by real-time quantitative PCR. Significant differences were observed in the expression of 12 immune-related genes after vaccination, suggesting variations in the sensitivity of each gene to bodily stimuli and its involvement in immunity. The specific immune mechanisms require further research. These results provide essential data for evaluating vaccines’ immune pathways and efficacy, offering optimism for vaccine development in fish immunology.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5588289","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ascidian Fouling in North Carolina Shellfish Farms","authors":"Jordan Pilcher,&nbsp;Grace Monteith,&nbsp;Brenna Hutchings,&nbsp;Susanna López-Legentil","doi":"10.1155/are/6629865","DOIUrl":"https://doi.org/10.1155/are/6629865","url":null,"abstract":"<div>\u0000 <p>Ascidians are marine sessile animals that are particularly abundant on artificial structures, where they often overgrow native species and compete with other filter feeders. North Carolina’s (NC) coastline supports productive aquaculture operations and ascidians are considered pests there. Five shellfish farms and four nearby marinas were surveyed to compare the structure and composition of their ascidian communities using photo-quadrat surveys and presence–absence and abundance matrices, with “marina” and “shellfish farm” as factors. Twelve species were observed in the marinas, but only six in the farms: three native, two cryptogenic, and one identifiable only to the genus level. The three native species (<i>Clavelina oblonga</i>, <i>Perophora viridis</i>, and <i>Molgula manhattensis</i>) have established populations in many world regions and were observed in at least two of the farms visited. The cryptogenic <i>Styela plicata</i> was found in all farms and marinas and was the most abundant species. All species observed in shellfish farms were also present in marinas, with no significant difference in ascidian composition or abundance. Independently of introduction status, species thriving in a wide range of habitats are more adaptable and better suited to establish populations on artificial substrates, resulting in increased maintenance costs for aquaculture operations.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6629865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NanoDet Model-Based Tracking and Inspection of Net Cage Using ROV","authors":"Yinghao Wu,&nbsp;Yaoguang Wei,&nbsp;Hongchao Zhang","doi":"10.1155/are/7715838","DOIUrl":"https://doi.org/10.1155/are/7715838","url":null,"abstract":"<div>\u0000 <p>Open sea cage culture has become a major trend in mariculture, with strong wind resistance, wave resistance, anti-current ability, high degree of intensification, breeding density, and high yield. However, damage to the cage triggers severe economic losses; hence, to adopt effective and timely measures in minimizing economic losses, it is crucial for farmers to identify and understand the damage to the cage without delay. Presently, the damage detection of nets is mainly achieved by the underwater operation of divers, which is highly risky, inefficient, expensive, and exhibits poor real-time performance. Here, a remote-operated vehicle (ROV)-based autonomous net detection method is proposed. The system comprises two parts: the first part is sonar image target detection based on NanoDet. The sonar constantly collects data in the front and middle parts of the ROV, and the trained NanoDet model is embedded into the ROV control end, with the actual output of the angle and distance information between the ROV and net. The second part is the control part of the robot. The ROV tracks the net coat based on the angle and distance information of the target detection. In addition, when there are obstacles in front of the ROV, or it is far away from the net, the D-STAR algorithm is adopted to realize local path planning. Experimental results indicate that the NanoDet target detection exhibits an average accuracy of 77.2% and a speed of approximately 10 fps, which satisfies the requirements of ROV tracking accuracy and speed. The average tracking error of ROV inspection is less than 0.5 m. The system addresses the problem of high risk and low efficiency of the manual detection of net damage in large-scale marine cage culture and can further analyze and predict the images and videos returned from the net. https://youtu.be/NKcgPcej5sI.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7715838","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, Characterization, and Expression Analysis of CD79A and CD79B in the Sichuan Taimen (Hucho bleekeri)","authors":"Yeyu Chen,&nbsp;Huanchao Yang,&nbsp;Xiaoyun Wu,&nbsp;Zhao Liu,&nbsp;Yanling Chen,&nbsp;Qinyao Wei,&nbsp;Jue Lin,&nbsp;Yi Yu,&nbsp;Quanyu Tu,&nbsp;Hua Li","doi":"10.1155/are/6688124","DOIUrl":"https://doi.org/10.1155/are/6688124","url":null,"abstract":"<div>\u0000 <p>In the immune system, humoral immunity is mediated by B lymphocytes that destroy antigens through the production of antibodies by plasma cells. The CD79A and CD79B play important roles in the transduction of antigen-stimulated signals. However, only a few studies have investigated the immune functions of CD79A and CD79B in teleosts. To understand the role of CD79 in teleosts, CD79A and CD79B were identified and functionally analyzed in a glacial relict <i>Hucho bleekeri</i>. CD79A and CD79B contain a typical signal peptide, an immunoglobulin domain, a transmembrane region, and a cytoplasmic tail. The amino acid sequence of <i>H. bleekeri</i> CD79 showed high homology with CD79 of salmonid fish. Phylogenetic analysis also showed that <i>H. bleekeri</i> CD79A and CD79B clustered with the CD79 of Salmonidae fish, including the genera <i>Salmo</i>, <i>Oncorhynchus</i>, and <i>Salvelinus</i>. Tissue expression analysis showed that <i>CD79A</i> and <i>CD79B</i> were widely expressed in all the tested tissues. The highest expression of <i>CD79A</i> and <i>CD79B</i> was found in the head kidney, spleen, and trunk kidney, whereas the lowest expression was observed in the stomach, skin, gonad, and muscles. After lipopolysaccharides and polyinosinic:polycytidylic acid stimulation, the expression levels of <i>CD79A</i> and <i>CD79B</i> in the head kidney and spleen of <i>H. bleekeri</i> changed significantly. Our study showed that CD79 may play a crucial role in the B-cell receptor signaling pathway and immune response in <i>H. bleekeri</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6688124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the Best Weaning Age to Dry Feeds and the Influence of Phospholipids and Long Chain Polyunsaturated Fatty Acids (LC-PUFAs) on Digestive Enzyme Activity and Growth Performance of African Bony-Tongue (Heterotis niloticus) Fry","authors":"Prince Ofori-Darkwah,&nbsp;Daniel Adjei-Boateng,&nbsp;Regina Esi Edziyie,&nbsp;Nelson Winston Agbo,&nbsp;Ivar Lund","doi":"10.1155/are/3858151","DOIUrl":"https://doi.org/10.1155/are/3858151","url":null,"abstract":"<div>\u0000 <p>Limited information exists on the dietary requirements of the African bony-tongue (<i>Heterotis niloticus</i>) fry as well as the suitable age to wean fry from live prey to formulated diets. These have been the major challenges to the commercial culture of the species. This study assessed the optimal weaning age of fry as well as the effect of varying phospholipid (PL) content and long chain polyunsaturated fatty acids (LC-PUFAs) on survival, performance, and digestive enzyme activity of <i>H. niloticus</i> fry. The two weaning diets used were formulated to be isonitrogenous, isolipidic, and isoenergetic (49% CP, 20% CL, and 22.7 MJ/kg). The diets differed only in the content of PLs and LC-PUFAs; eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Soy lecithin (PLs) and Algatrium DHA70 (a glyceride product high in DHA and EPA) were included at two levels. The diets were, respectively, labeled low phospholipid (LPP) (formulated to contain: 3.16% PLs, LC PUFAs: 0.66% DHA 0.10% EPA) and high phospholipid (HPP) (formulated to contain: 12.96% PLs, LC PUFAs: 3.04% DHA, 0.75% EPA). African bony-tongue fry were fed <i>Artemia</i> nauplii until 15, 25, 35, 45, 65, or 85 days after hatching (DAH), respectively, then cofed with <i>Artemia</i> and one of the two weaning diets for an additional 7 days after which <i>Artemia</i> feeding was completely withdrawn. Hereafter, the fry were maintained solely on the experimental diets for 10 days. A control group was kept on <i>Artemia</i> nauplii throughout the experiment. The results showed that the survival rate of fish on the LPP and HPP diets increased significantly (<i>p</i>  &lt; 0.05) with weaning age; from 0% (LPP and HPP) for fry weaned at 15 DAH to a survival rate of 69.4% ± 3.9% (LPP) and 80.0% ± 9.4% (HPP) at 65 and 85 DAH, respectively. This was, however, lower than the survival rate obtained for the control (<i>Artemia</i>) which was 93.3% ± 3.33% at 85 DAH. The final mean weight, weight gain, and survival rate improved significantly (<i>p</i>  &lt; 0.05) for fry fed the HPP diet compared to the LPP diet. All digestive enzyme activity decreased significantly with increasing fish age (<i>p</i>  &gt; 0.05). Based on the results, it is recommended, that <i>H. niloticus</i> fry is maintained on live <i>Artemia</i> nauplii and provided with dry feeds high in LC-PUFAs (33.98 mg g⁻¹ diet) and PLs (12.96 mg g⁻¹ diet) from &lt;65 DAH to obtain higher survival rates.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3858151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing of EhAQP1, EhPTP2, and EhTK Genes in Ecytonucleospora Hepatopenaei Using RNA Interference: A Potential Strategy for Preventing and Treating EHP Infection in Shrimp","authors":"Tingyue Liu,&nbsp;Yi Qiao,&nbsp;Huiwen Jiang,&nbsp;Xiaohui Cao,&nbsp;Jie Cheng,&nbsp;Ge Jiang,&nbsp;Xiaoman Sun,&nbsp;Hui Shen","doi":"10.1155/are/3226686","DOIUrl":"https://doi.org/10.1155/are/3226686","url":null,"abstract":"<div>\u0000 <p><i>Ecytonucleospora hepatopenaei</i> (EHP) is an obligate intracellular parasitic pathogen of shrimp, causing growth retardation and increased susceptibility to opportunistic infections. Currently, there are few reports on the prevention and treatment of EHP infection in shrimp, and research on its drug targets is limited. This article utilizes RNA interference (RNAi) technology to conduct knockdown verification studies on potential drug targets involved in EHP’s invasion mechanism and life cycle. Three target gene sequences from genome, and annotated as <i>EhAQP1</i> (aquaporin 1), <i>EhPTP2</i> (polar tube protein), and EhTK (thymidine kinase) were used to synthesize specific small interfering RNA (siRNA). These siRNAs were injected into EHP-infected shrimp, and the hepatopancreas was sampled at 12, 24, 48, and 72 h postinjection to verify the interference effect and spore load. The results showed that RNAi exhibits varying inhibitory effects on different genes and is time-dependent. For EhAQP1, interference was most effective in the early stages (12 to 24 h), while the interference effects for EhTK were more pronounced at 48 and 72 h, the expression of EhPTP2 was significantly downregulated (<i>p</i> &lt; 0.001) only at 24 h. In the EhAQP1 and EhPTP2 interference group, the spore load decreased from 12 to 48 h, but increased at 72 h. In contrast, the EhTK interference group showed a continuous decrease in spore load from 12 to 72 h. In summary, this study provides a new strategy for prevention and treatment of shrimp EHP infection and lays the foundation for subsequent research on RNAi–based prevention and treatment of EHP infection.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3226686","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Behavior in Odontobutis potamophila (Günther, 1861)","authors":"Miao Xiang,&nbsp;Li Li,&nbsp;Hongliang Xu,&nbsp;Bo Li,&nbsp;Hongxi Guo,&nbsp;Zhanhu Yang,&nbsp;Chuankun Zhu,&nbsp;Man Wang,&nbsp;Jie Wang,&nbsp;Wei Xin,&nbsp;Songguang Xie,&nbsp;Xiaoming Zhu,&nbsp;Lei Zhang","doi":"10.1155/are/8860515","DOIUrl":"https://doi.org/10.1155/are/8860515","url":null,"abstract":"<div>\u0000 <p>An animal’s behavior can directly reflect its psychological or physiological condition. The in-depth understanding of fish behavior, especially reproductive behavior, is an important safeguard to promote sustainable aquaculture. In this study, we used the Posture-Act-Environment (PAE) coding system to construct an ethogram of <i>Odontobutis potamophila</i> to help people better judge and identify <i>O. potamophila</i> behaviors; and we designed two groups of experiments to figure out the individual factors affecting the reproduction of <i>O. potamophila</i>. These were the selection of female <i>O. potamophila</i> for male <i>O. potamophila</i> of different sizes, with or without nests, and with or without parental care behaviors, as well as the selection of male <i>O. potamophila</i> for female <i>O. potamophila</i> of different sizes. The results showed that we recorded 14 postures, 22 movements, and 29 behaviors of <i>O. potamophila</i>. Based on their biological functions, the recorded behaviors of <i>O. potamophila</i> were divided into nine types, that is, exploration, territoriality, attack, courtship, mating, parental care, ingestion, stationary behavior, and others. <i>O. potamophila</i> had nocturnal habits, and all the nighttime activities were significantly higher than those during the daytime (<i>p</i> &lt; 0.001). The mating system of <i>O. potamophila</i> was polygamous, and the average mating duration for successful spawning was 9.99 ± 1.23 h. In mate choice experiments, both females and males spent significantly more time stationary than activity (<i>p</i> &lt; 0.001). Females exhibited a slight preference for males with large individuals (strength of preference [SOP] = 53.46%), no nests (SOP = 50.51%), and no parental care behavior (SOP = 55.58%). The results of male mate choice were similar. Although the standard length of females was positively correlated with fecundity (<i>r</i> = 0.61), there were no significant differences in the number of male-to-female choices or duration of association (<i>p</i> &gt; 0.05). This indicated that males showed no preference for larger or high-fertility females (SOP = 47.10%). Therefore, it is likely that males may not be selective for females with different fecundity levels. This study enhances the current understanding of the behavioral patterns of <i>O. potamophila</i>. It establishes a foundation for further research on its reproductive behavior and contributes to the advancement of captive breeding for this species.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8860515","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of Voliam Flexi on Antioxidant Responses, Oxidative Stress, and Histopathological Changes in Juvenile African Catfish (Clarias gariepinus)","authors":"Mohamed Hamed,&nbsp;Hanem S. Abdel-Tawab,&nbsp;Mostafa A. Abdel-Maksoud,&nbsp;Abdul Malik,&nbsp;Sabiha Fatima,&nbsp;Christopher J. Martyniuk","doi":"10.1155/are/8608065","DOIUrl":"https://doi.org/10.1155/are/8608065","url":null,"abstract":"<div>\u0000 <p>This study investigated the toxicological impact of Voliam Flexi (containing chlorantraniliprole and thiamethoxam) on juvenile African catfish (<i>Clarias gariepinus</i>), a species commonly exposed to pesticides in rice cultivation regions. This study investigated the toxic effects of Voliam Flexi on juvenile African catfish, focusing on oxidative stress, antioxidant enzyme disruption, and tissue damage at environmentally relevant concentrations. Over a 15-day period, fish were exposed to environmentally relevant pesticide concentrations commonly applied in Egyptian rice fields, specifically 43.5, 87.5, and 175 mg/L. Antioxidant defense mechanisms, including GST (glutathione S-transferase), SOD (superoxide dismutase), CAT (catalase), and TAC (total antioxidant capacity), were measured alongside oxidative damage indicators such as lipid peroxidation (LPX) and DNA fragmentation. A dose-dependent reduction in antioxidant enzyme activity and an increase in oxidative damage were observed, with higher pesticide concentrations leading to elevated LPX levels and DNA fragmentation. Histopathological evaluations of the kidney and spleen revealed significant degenerative changes, such as tubular damage, edema, and pyknotic nuclei, particularly at higher concentrations. These findings suggest that Voliam Flexi poses considerable risks to aquatic organisms, disrupting cellular functions, and leading to tissue damage. The study highlights the potential threat of pesticide runoff to aquatic biodiversity and calls for stricter environmental regulations to mitigate these risks.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8608065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}