Aquaculture Research最新文献

{"title":"Partial Replacement of Fish Meal With Shrimp Waste Meal: Effects on Growth, Digestibility, and Immunity in Juvenile Beluga Sturgeon (Huso huso)","authors":"Amirsoheil Taheri,&nbsp;Abdolmohammad Abedian Kenari,&nbsp;Maryam Aftabgard","doi":"10.1155/are/5469830","DOIUrl":"https://doi.org/10.1155/are/5469830","url":null,"abstract":"<div>\u0000 <p>This study aimed to investigate the effects of replacing different levels of dietary shrimp waste meal (SWM) with fish meal (FM) on growth performance (GP), carcass composition (CC), apparent digestibility (AD), and innate immunity of juvenile beluga sturgeon (<i>Huso huso</i>). A total of 180 juvenile beluga with an average weight of 130.0 ± 6.5 g were assigned to five treatments (with three replicates each): SWM0 (control group), SWM5, SWM10, SWM15, and SWM20, corresponding to 0%, 5%, 10%, 15%, and 20% inclusion of SWM in place of FM in their diets for 8 weeks. The results showed that GP and protein AD declined in the SWM5 group (<i>p</i> &lt; 0.05), while no significant differences were observed in GP and AD across the other treatments compared to the control (<i>p</i> &gt; 0.05). Regarding CC, no significant differences were found among the treatments. The fatty acid (FA) profile of the muscles in juvenile beluga fed with SWM5–20 was similar to that of the control group, with the only notable difference being a decrease in eicosapentaenoic acid (EPA) as dietary SWM levels increased (<i>p</i> &lt; 0.05). The essential amino acid (EAA) content also decreased in the SWM20 group (<i>p</i> &lt; 0.05), although the EAA-to-nonessential amino acid (NEAA) ratio in the SWM5–20 treatments was not significantly different from that of the control group (<i>p</i> &gt; 0.05). The highest levels of serum lysozyme (LYZ) and alternative complement hemolytic (ACH50) activity were observed in the SWM15 group, with the lowest levels found in the control group (<i>p</i> &lt; 0.05). The results showed that substituting FM with SWM at a rate of 15%–20% in the diet had no significant and negative impact on GP, nutritional value, or digestibility. On the contrary, it boosted the immune system of juvenile beluga sturgeon (<i>H. huso</i>). The positive immunostimulation appears to be related to the beneficial effects of amino acid (AA) or imino acids (IAs) and chitin as well as their recognition by mannose/fucose receptors attached to white blood cells.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5469830","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Acute and Long-Term Hypoxia and Hyperoxia on Antioxidant Metabolism and Gene Expression in Juvenile Rainbow Trout (Oncorhynchus mykiss)","authors":"Abdullah Tunç,&nbsp;Orhan Erdoğan,&nbsp;Onur Vural,&nbsp;Ercüment Aksakal","doi":"10.1155/are/6628284","DOIUrl":"https://doi.org/10.1155/are/6628284","url":null,"abstract":"<div>\u0000 <p>Aquatic organisms experience oxidative stress due to environmental stressors that promote oxygen exchange, such as eutrophication, algal photosynthetic activity, or changes in water temperature. This study aimed to clarify the effects of oxidative stress on the liver of juvenile rainbow trout (<i>Oncorhynchus mykiss</i>) exposed to hypoxia and hyperoxia by examining the physiological roles of hydrogen peroxide, glutathione (GSH) redox status, malondialdehyde (MDA) concentration, antioxidant enzyme activity, and antioxidant gene expressions. Dissolved oxygen levels were maintained at 4.0 ± 0.5 mg/L for hypoxia, 7.5 ± 0.5 mg/L for normoxia, and 12 ± 1.2 mg/L for hyperoxia. Liver samples were collected from each experimental group following exposure (6, 12, 24, 48, and 72 h) and chronic exposure (28 days). Under both hypoxia and hyperoxia conditions, reduced GSH levels and the oxidative stress index (OSI) decreased compared to normoxia (control group), whereas oxidized glutathione (GSSG) levels, the GSH/GSSG ratio, and MDA concentrations increased. Hydrogen peroxide levels were unstable. Superoxide dismutase (SOD) activity remained consistently lower than that of the control group. Catalase (CAT) activity decreased with chronic exposure to hyperoxia. Glutathione peroxidase (GPx) activity fluctuated with varying oxygen treatments. Glutathione-S-transferase (GST) activity increased after 12 h of hypoxia exposure but decreased with acute hyperoxia; however, chronic hyperoxia exposure eventually increased GST activity. Glutathione reductase (GR) activity is generally reduced under hypoxia. The expressions of SOD and CAT increased at 24 h but decreased at other times. GPx expression increased under chronic hypoxia but decreased under hyperoxia, while GST expression decreased with chronic treatments. Hypoxia and hyperoxia influence the antioxidant defense system in the fish liver through different pathways. While a coordinated relationship between gene expression and enzyme activities is observed under acute exposures, this coordination diminishes during chronic exposures, leading to the depletion of defense mechanisms. This suggests that the capacity of aquatic organisms to adapt to oxidative stress is limited and that post-transcriptional mechanisms play a significant role in regulating antioxidant responses.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6628284","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Analysis of the Growth Performance, Morphological Indicators, and Transcriptome Between Two Coastal Regions of China in the Mud Crab (Scylla paramamosain)","authors":"Yue Wang,&nbsp;Xiao-Shan Wang,&nbsp;Sheng-Yu Liu,&nbsp;Ben-Jian Wang,&nbsp;Zhi-Xing Su,&nbsp;Xiao-Kang Lv,&nbsp;Jia-Yuan Xu,&nbsp;Xue-Feng Song,&nbsp;Bian-Bian Zhang,&nbsp;Li-Guo Yang","doi":"10.1155/are/6508890","DOIUrl":"https://doi.org/10.1155/are/6508890","url":null,"abstract":"<div>\u0000 <p>The mud crab (<i>Scylla paramamosain</i>), a commercially important species in China, is widely distributed along the southeastern coast. This study compares the growth performance, morphological traits, and transcriptomic profiles of two geographically distinct populations from Sanmen County, Zhejiang (SMC) and Zhangpu County, Fujian (ZPC). Juvenile crabs collected from both regions were reared in ponds under identical conditions for 7 months. Key results showed that the SMC population exhibited significantly higher daily weight gain, specific growth rate (SGR), and propodus-to-cheliped length ratio compared to ZPC (<i>p</i> &lt; 0.01 for both sexes). Transcriptomic analysis revealed 44 consistently upregulated differentially expressed genes (DEGs) in SMC individuals. Among these, <i>Otop</i> (Otopetrin 1), <i>Smyd</i> (SET and MYND domain containing), <i>AM1159</i> (<i>Cancer pagurus</i> Cuticle protein AM1159), <i>PPAF1</i> (PPAR gamma coactivator 1 <i>α</i>), and <i>NARG2</i> (NMDA receptor regulated 2) were functionally linked to molting and growth regulation in crustaceans. Notably, two mTOR pathway-associated genes (<i>QDPR</i> [Quinoid dihydropteridine reductase] and <i>NLRC 3</i> [NLR family CARD domain containing 3]) and several energy/nutrient metabolism genes were also upregulated in SMC. Enrichment analysis identified four active KEGG pathways in SMC: “Melanogenesis,” “Riboflavin metabolism,” “Choline metabolism,” and “Ribosome biogenesis.” These findings suggest that the superior growth performance of SMC crabs may stem from enhanced molting efficiency and nutrient allocation mechanisms. While <i>QDPR</i> and <i>NLRC3</i> are hypothesized to mediate mTOR-driven metabolic regulation, further functional validation is required to establish their causative roles. This study provides molecular markers (<i>Otop</i>, <i>Smyd</i>, etc.) for selective breeding programs and a transcriptomic framework for understanding regional adaptation in marine crustaceans.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6508890","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detailed Early Life History of the Giant Muricid Snail Chicoreus ramosus in the Southern China Sea","authors":"Gan Wang,&nbsp;Yu Zhong,&nbsp;Yanping Qin,&nbsp;Qishuai Wang,&nbsp;Jingyue Huang,&nbsp;Chao Yue,&nbsp;Weitao Wan,&nbsp;Haitao Ma,&nbsp;Jun Li,&nbsp;Xiangyong Yu,&nbsp;Yuehuan Zhang","doi":"10.1155/are/4342005","DOIUrl":"https://doi.org/10.1155/are/4342005","url":null,"abstract":"<div>\u0000 <p>Tropical marine life is highly diverse, but the development and utilization of many species remain insufficient. The giant muricid snail <i>Chicoreus ramosus</i>, notable for its large size and high-quality meat, holds significant potential for aquaculture. Previous descriptions of its seedling production were incomplete and sometimes inaccurate. This study details the early life history and aquaculture potential of <i>C. ramosus</i>. Experiments conducted from December 2023 to October 2024 covered parental cultivation, spawning, and developmental tracking. Key findings include female parents spawning 191–531 egg capsules per event, each containing 189–520 fertilized eggs, with up to three spawnings and decreasing capsule numbers in subsequent events. The early life stages are: (1) Inner egg capsule development period: Fertilized eggs progress through stages including polar body release, two-cell, four-cell, eight-cell, morula, blastocyst, gastrula, and veliger larvae stages; (2) Outer egg capsule development: Postveliger larvae emerge and transition to juvenile and youth stages. Specifically, eggs develop into middle veliger larvae in about 4 weeks, followed by a 1–2 week postveliger stage, juvenile metamorphosis around 2 weeks later, and growth into the youth stage in 2–3 weeks. This study provides a successful framework for artificial incubation and seedling production, advancing the potential aquaculture applications of <i>C. ramosus</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4342005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Dietary Arachidonic Acid on the Broodstock Productive Performance and Egg Quality of Largemouth Bass (Micropterus nigricans)","authors":"Hanlin Xu,&nbsp;Biao Yun,&nbsp;Zexia Gao,&nbsp;Jian Wang","doi":"10.1155/are/3091729","DOIUrl":"https://doi.org/10.1155/are/3091729","url":null,"abstract":"<div>\u0000 <p>The influence of dietary arachidonic acid (ARA) on the reproductive performance of broodstock fish is a critical area of aquaculture research. This study investigates the impact of ARA levels in broodstock diets on broodstock hormone, fecundity, egg quality, and larval survival of largemouth bass (<i>Micropterus nigricans</i>). Experimental diets containing 0.9%, 2.0%, and 3.6% ARA as a percentage of total fatty acids (TFAs) were formulated and administered to broodstock over 84 days. Results indicated that a dietary ARA level of 3.6% significantly increased vitellogenin content in broodstock serum while reducing serum estradiol levels. Despite this hormonal shift, reproductive performance metrics, including fecundity, egg hatch rates, and the number of viable eggs until mouth opening, were not significantly affected by the ARA content in the broodstock diet. However, the 3.6% ARA group showed notable improvements in egg size and higher concentrations of ARA and docosahexaenoic acid in the eggs. Based on these results, 0.9% ARA in TFA content in broodstock diet could be sufficient for the reproduction of largemouth bass. A level of 3.6% ARA of TFAs can be added to the diet of largemouth bass broodstock to enhance offspring potential growth performance and healthy status.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3091729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Analysis of Embryonic Development of Embryos From the Crossing of Two Species of Siluriform","authors":"Yasmin Santos dos Reis,&nbsp;Stella Indira Rocha Lobato,&nbsp;Laicia Carneiro-Leite,&nbsp;Lais Pedroso Borges,&nbsp;Lorena Pacheco da Silva,&nbsp;Rodrigo Yutaka Dichoff Kasai,&nbsp;Rosicleire Veríssimo-Silveira,&nbsp;Alexandre Ninhaus-Silveira","doi":"10.1155/are/5566265","DOIUrl":"https://doi.org/10.1155/are/5566265","url":null,"abstract":"<div>\u0000 <p>Based on the need for knowledge about the biology of hybrid fish specimens currently being produced by commercial fish farmers, this study aimed to study the initial ontogenesis up to the fifth day after larval hatching of the Hybrid LP produced by crossing <i>Leiarius marmoratus</i> (♀) and <i>Pseudoplatystoma reticulatum</i> (♂), thus producing important data for fish farming, as well as for ichthyoplankton studies. The embryogenesis of the Hybrid LP was typical of teleosts but with differences and similarities in relation to other species and their parents, such as duration of development and moment of mesoderm segmentation, among others. Five stages of embryonic development were defined, from the moment of fertilization until larval hatching: zygote, cleavage, gastrulation, organogenesis, and hatching. The embryogenesis period was 13 h, at an average temperature of 27.98°C. The embryonic development stages established for the hybrid were like those of its parental species <i>L. marmoratus</i> and <i>P. reticulatum</i>. Thus, through this research, it was possible to conclude that the embryonic development of the Hybrid LP originating from interspecific crosses between females of <i>L. marmoratus</i> with males of <i>P. reticulatum</i> occurred in a development period similar to that found in studies of its parental species as well as for the inverse hybrid (PL), originating from females of <i>P. reticulatum</i> with males of <i>L. marmoratus</i>, under similar temperatures, not being possible to morphologically differentiate the embryos and larvae until the fifth day after hatching, neither of the parental species nor the Hybrid PL.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5566265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity and Nutritional Quality Comparison in Schizothorax waltoni, Schizothorax o’connori, and Their Different Maternal Natural Hybrids","authors":"Bin Xu,&nbsp;Liang Peng,&nbsp;Tianyi Zhao,&nbsp;Benhe Zeng,&nbsp;Liqiao Zhong,&nbsp;Huke He,&nbsp;Yali Yu,&nbsp;Kaijin Wei,&nbsp;Baoshan Ma","doi":"10.1155/are/8812358","DOIUrl":"https://doi.org/10.1155/are/8812358","url":null,"abstract":"<div>\u0000 <p>Natural hybridization plays an important role in species evolution, and its heterosis also provides inspiration for breeding. The present study is to compare the genetic diversity and muscle nutritional quality of <i>Schizothorax waltoni</i>, <i>Schizothorax o’connori</i>, and their natural hybrids that are naturally distributed in the Yarlung Zangbo River. Based on the cluster analysis, the genetic relationship between 13 hybrids (namely <i>S. w. ♀</i> hybrid) and <i>S. waltoni</i> was found to be closely related, whereas 24 hybrids (namely <i>S. o. ♀</i> hybrid) were closely related to <i>S. o’connori</i>. The haplotype polymorphisms across the four populations varied between 0.847 and 0.936, while the nucleotide polymorphisms were 0.00305–0.00418. The genetic diversity of the two hybrids was higher than that of <i>S. waltoni</i> and <i>S. o’connori</i>. The crude fat content of the two natural hybrids is significantly higher than that of <i>S. waltoni</i> and <i>S. o’connori</i>. The total amino acid (TAA), essential amino acid (EAA), and delicious amino acid (DAA) contents and the EAA index (EAAI) in the two hybrids were greater than those in <i>S. waltoni</i> and <i>S. o’connori</i>. Compare to <i>S. waltoni</i> (22) and <i>S. o’connori</i> (18), the two natural hybrids (both 24) showed the richer types of fatty acids. The highest contents of polyunsaturated fatty acids (PUFAs), <i>ω</i>-3 PUFAs, and <i>ω</i>-6 PUFAs were observed in <i>S. waltoni</i>, and <i>ω</i>-6 PUFA within the two hybrids possessed intermediate characteristics. In conclusion, the hybrids of <i>S. waltoni</i> and <i>S. o’connori</i> showed advantages of growth rate, genetic diversity, and nutrition quality, therefore appearing to have a potential of breeding for further development and utilization.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8812358","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Sequencing and Functional Analysis of Klebsiella Strain mr-1S Originating From Macrobrachium rosenbergii","authors":"Yang Xu,&nbsp;Ying Zhao,&nbsp;Haihua Cheng,&nbsp;Fei Peng,&nbsp;Qiang Gao","doi":"10.1155/are/3407093","DOIUrl":"https://doi.org/10.1155/are/3407093","url":null,"abstract":"<div>\u0000 <p><b>Background:</b><i>Klebsiella</i> mr-1S, a newly emerging pathogen that poses a substantial threat to public health by inducing severe infectious diseases, has been detected in <i>Macrobrachium rosenbergii</i> for the first time in our prior investigation. Given its potential for environmental adaptation and genomic evolution, this study aims to delve into these aspects to better understand its pathogenicity and evolutionary trajectory.</p>\u0000 <p><b>Methods:</b> In this study, the <i>Klebsiella</i> isolates were subjected to routine cultivation in a tryptic soya broth (TSB) medium. Subsequently, the genomic DNA of each isolate was individually extracted and analyzed. Prior to genomic sequencing, the integrity and concentration of the DNA samples were meticulously evaluated to ensure the accuracy and reliability of the subsequent sequencing process.</p>\u0000 <p><b>Results:</b> The genomic sequence of <i>Klebsiella</i> mr-1S was deciphered, revealing a length of 5,143,806 base pairs with a GC content of 54.97%. Remarkably, the genome encompasses a multitude of putative mobile genetic elements (MGEs), including 43 genomic islands (GIs) and 2 prophages. These elements confer upon the bacterium crucial adaptive attributes such as resistance, virulence, and metabolic capabilities. Notably, the identification of prophage-associated clusters originating from the genus Pseudomonas suggests a potential horizontal gene transfer (HGT) mechanism mediated by phages within Pseudomonas, highlighting the complex genetic interactions between different bacterial species.</p>\u0000 <p><b>Conclusion:</b> In addition, the presence of two genes encoding CRISPR-Cas proteins within the <i>Klebsiella</i> mr-1S genome indicates the existence of a functional CRISPR-Cas system in this bacterium. This finding implies that during its evolutionary history, Klebsiella mr-1S may have developed mechanisms to evade host immune recognition, thereby facilitating efficient HGT and enhancing its adaptability and survival capabilities in diverse environments. Overall, this study provides novel insights into the environmental adaptability and genomic plasticity of <i>Klebsiella</i> from <i>Macrobrachium rosenbergii</i>, underscoring its potential as an emerging pathogen in aquatic ecosystems and paving the way for future research on its pathogenic mechanisms and potential control strategies.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3407093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastic Pollution in Indigenous Fish From the Padma River, Bangladesh: A Case Study","authors":"Md. Tarek Mondal,&nbsp;Md. Aslam Khan,&nbsp;M. Golam Mortuza","doi":"10.1155/are/9917204","DOIUrl":"https://doi.org/10.1155/are/9917204","url":null,"abstract":"<div>\u0000 <p>Microplastics (MPs) are prevalent in aquatic ecosystems; however, inland water bodies have received relatively little attention. These ecosystems provide essential habitat for various fish species, which contribute to ecological equilibrium and support the livelihoods and food security of millions. Fish demonstrate increased vulnerability to microplastic ingestion, making them essential bioindicators of ecosystem health and contamination levels. This study evaluates the presence of MPs in the intestine, gills, and muscle tissue of 30 individuals belonging to five commonly found fish species (<i>Rhinomugil corsula</i>, <i>Mystus tengara</i>, <i>Ompok pabda</i>, <i>Labeo bata</i>, and <i>Cirrhinus mrigala</i>) in the Padma River. A total of 252 MP items of different shapes were recorded in 100% of individuals investigated, exhibiting an average frequency of 8.27 ± 2.16 items per individual. Among the fish species, <i>Ompok pabda</i> exhibited the highest ingestion of MPs, with an average of 11.5 ± 1.87 items per individual, followed by other species. Organ-wise analysis of MPs trend was found to be: intestine &gt; gill &gt; muscle. Fiber was recognized as the most commonly consumed MP type (72%), followed by fragment (22%), pellet (4%), and foam (2%). The white-color MP (24%) was predominant, followed by other colored MPs. The majority of MP particles were found in the size class 1–5 mm (41.10% ± 12.34%), followed by &lt;500 μm (34.18% ± 16.34%) and 500 μm–1 mm (24.72% ± 11.51%). Fourier transform infrared (FT-IR) spectroscopy investigation confirms both high- and low-density polyethylene polymeric compositions of MPs. The study assesses the prevalence of microplastics in Padma River fish, highlighting the immediate necessity for focused conservation initiatives, ongoing monitoring, and evaluation of microplastic pollution to protect aquatic ecosystems and human health.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9917204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Coexposure Effects of Pyrogallol and Microplastic on the Red Swamp Crayfish Procambarus clarkii","authors":"Rashad E. M. Said,&nbsp;Mohamed Hamed,&nbsp;Walaa M. Shaalan,&nbsp;Heba Allah M. Elbaghdady,&nbsp;Alaa El-Din H. Sayed","doi":"10.1155/are/6084150","DOIUrl":"https://doi.org/10.1155/are/6084150","url":null,"abstract":"<div>\u0000 <p>Investigating the impact of two or more toxic chemicals on aquatic organisms is one of the main objectives of toxicological research. Microplastics (MPs) are identified as a carrier or vector for other pollutants. Therefore, the current study investigated the effects of both pyrogallol (PG) and MPs, individually and in combination, on freshwater crayfish (<i>Procambarus clarkii</i>). Following a 15-day exposure to 10 mg/L of PG and100 mg/L MPs and their combination (10 mg/L PG + 100 mg/L MPs), the hemocyte count, hepatopancreatic parameters, antioxidant variables, and histopathological markers were all assessed in the crayfish. The results revealed that PG and MPs potentially individually or combined altered the hemocyte count (including granular and semigranular) compared to control group (0.001 ≥ <i>p</i> &lt; 0.0001). The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and protein showed significant elevation (<i>p</i> &lt; 0.05) in PG–MP-exposed fish compared to control ones. In comparison to control <i>P. clarkii</i>, the measured oxidation indicators, superoxide dismutase (SOD), glutathione (GSH), and total antioxidant capacity (TAC), showed a significant reduction after single exposure to PG and MPs (0.001 &gt; <i>p</i> &lt; 0.0001), while catalase (CAT) and malondialdehyde (MDA) elevated drastically (0.05 &gt; <i>p</i> &lt; 0.0001). The histology of the hepatopancreas has shown many deformities and abnormalities after individual or combined toxicity, such as vacuolations, degraded hepatopancreatic tubules, eosinophilic deposits, hemocytic infiltrations, and aberrant tubules. Accordingly, there is a synergistic relationship between PG and MP toxicity to crayfish. Therefore, it is crucial to routinely check and assess the discharge of waste into waterways.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6084150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}