Aquaculture Research最新文献

{"title":"Use of Chemical–Physical and Natural Ingredients of Food Factors Modulation for Freshwater Aquaculture Systems Fish Wellness: A Systematic Review","authors":"Eloise Pulvirenti,&nbsp;Claudia Favara,&nbsp;Paola Rapisarda,&nbsp;Maria Castrogiovanni,&nbsp;Carmen Sica,&nbsp;Erica Aquilia,&nbsp;Antonio Cristaldi,&nbsp;Gea Oliveri Conti,&nbsp;Margherita Ferrante","doi":"10.1155/are/1284780","DOIUrl":"https://doi.org/10.1155/are/1284780","url":null,"abstract":"<div>\u0000 <p>Freshwater aquaculture plays a crucial role in addressing the growing demand for seafood, providing a sustainable solution, and contributing to global food security. It offers notable advantages for saltwater aquaculture in terms of cost-effectiveness, environmental management, and reduced ecological impact. As a system for farming aquatic organisms, aquaculture significantly contributes to human food production and addresses issues like protein shortages and diet-related diseases. However, it also faces challenges such as fish epidemics and ecosystem impacts. This Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA)-based systematic review focuses on the needs of both the modulation of chemical–physical environmental factors and natural ingredients used in feed formula to improve the wellness of farmed fish in freshwater aquaculture. We analyzed published articles between 1 January 2013 and 30 June 2024 using PubMed, Scopus, and Web of Science databases. The results highlighted the good effects of ultraviolet light (UV-C) treatment about bacterial infections and the use of oxolinic acid (OA) and alpha-lipoic acid (ALA) on fish welfare through diet. These factors highlighted the importance of dose-dependent applications of both environmental physico-chemical factors and natural feed ingredients. Tailoring diets with specific nutritional components is crucial to improve productive outcomes in freshwater aquaculture systems, ultimately supporting the health and welfare of farmed fish.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1284780","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity of Oreochromis shiranus (Boulenger 1905) After Six Consecutive Generations of Selective Breeding Compared With Corresponding Wild Populations","authors":"Thomson Sanudi,&nbsp;Clint Rhode,&nbsp;Rouvay Roodt-Wilding,&nbsp;Alfred Maluwa","doi":"10.1155/are/4440384","DOIUrl":"https://doi.org/10.1155/are/4440384","url":null,"abstract":"<div>\u0000 <p>Aquaculture in sub-Saharan African accounts for less than 2% of world aquaculture production. The use of unimproved species limits the growth of aquaculture. In order to increase production, there is a need to develop aquaculture species that show vigor and adapt to different culture conditions. A genetic improvement program for <i>Oreochromis shiranus</i> was initiated at the National Aquaculture Center (NAC), in Zomba, Malawi. The program used pedigree records during mate allocation to avoid mating of siblings. After six consecutive generations of selection, a sample of 32 fish was taken for genetic analysis using a panel of 16 microsatellite markers. Genetic diversity and population differentiation indices of the sixth filial generation (F6) were compared with wild fish populations sampled from the corresponding locations from which the initial brood stock was collected. The study results show that the genetic diversity of the F6 generation and wild populations was not significantly different after the Kruskal–Wallis tests. However, the number of private alleles was lower in generation F6 (0.80) than in the overall average for wild populations (1.28). Discriminant analysis of principal components (DAPCs) supported analysis of molecular variance (AMOVA) by showing that there were no significant overlaps of inertia ellipses among F6 generation and wild populations. The experimental population differentiated into a separate group because of the random brood stock contribution from only a few fish from the wild population. The results suggest that the initial genetic diversity of the brood stock was sufficient as shown by the high number of alleles and polymorphic information content (PIC) values which contributed to the success of the selection program. The selection program successfully produced large fish at harvest and ensured genetic diversity of the F6 generation.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4440384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BiFormer Attention-Guided Multiscale Fusion Mask2former Networks for Fish Abnormal Behavior Recognition and Segmentation","authors":"Jihang Liu,&nbsp;Zeyuan Hu,&nbsp;Yixi Zhang,&nbsp;Yinjia Li,&nbsp;Jinsong Yang,&nbsp;Hong Yu","doi":"10.1155/are/8892810","DOIUrl":"https://doi.org/10.1155/are/8892810","url":null,"abstract":"<div>\u0000 <p>To address the issues of accurately identifying and tracking individual fish abnormal behaviors and poor adaptability in the aquaculture field, this paper proposes a Mask2former model combined with a bidirectional routing attention mechanism (BiFormer) and a multiscale dilated attention (MSDA) module for fish abnormal behavior recognition and segmentation. To compensate for the lack of publicly available datasets on fish abnormal behavior, we created the “FISH_segmentation_2023” abnormal behavior dataset, which includes four types of fish behaviors. First, by introducing the BiFormer attention mechanism, the model can better capture critical temporal and spatial information in image sequences, significantly enhancing feature representation. Second, after processing the feature maps with the pixel decoder, the MSDA module is introduced to perform multiscale fusion on these features. The fused features are then passed to the transformer decoder, further enhancing the model’s ability to recognize fish abnormal behaviors. Finally, to further improve model performance and address class imbalance issues in the dataset, we designed a composite loss function combining focal loss and dice loss (FD loss). This loss function can balance the influence of easy and difficult-to-classify samples while optimizing segmentation performance, thereby improving the model’s recognition accuracy and mean intersection over union (mIoU) metrics. Experimental results show that the BiFormer multiscale dilated attention FD loss (BMF)-Mask2former model exhibits high performance, achieving average intersection over union (IoU), accuracy, and recall values of 92.33%, 95.63%, and 94.82%, respectively, on the self-built FISH_segmentation_2023 dataset, representing improvements of 6.10%, 4.50%, and 5.09%, respectively, compared to the Mask2former model. The study demonstrates that the proposed model can accurately capture both local and contextual features of fish abnormal behaviors through multiscale fusion methods, resulting in high-quality segmentation outcomes.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8892810","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Multifaceted Role of Gut Microbiota in Sea Urchin Digestion: Diversity, Function, Symbiosis, and Carbohydrate Degradation","authors":"Yining Zheng,&nbsp;Furong Wang,&nbsp;Yu Jiang,&nbsp;Yu Zhang,&nbsp;Xin Wang,&nbsp;Lianshun Wang,&nbsp;Li Wang,&nbsp;Yuan Wang,&nbsp;Yanan Lu,&nbsp;Yuting Cong,&nbsp;Guojun Yang","doi":"10.1155/2024/7363987","DOIUrl":"https://doi.org/10.1155/2024/7363987","url":null,"abstract":"<div>\u0000 <p>Sea urchins, ecologically important marine species, host a complex gut microbiota that plays a significant role in their digestion, nutrient acquisition, and overall health. The gut microbes, along with endogenous enzymes, facilitate the breakdown of macroalgae and other food sources. Environmental factors, including temperature, salinity, and habitat type, can greatly influence the microbial communities within sea urchins. Dietary variations can also lead to shifts in the abundance and composition of intestinal bacteria, highlighting the strong connection between diet and gut microbiota. The sea urchin’s unique anatomy and digestive system adaptations contribute to their ability to process and absorb nutrients efficiently, supporting their varied diets. Gut microbes not only participate in the decomposition of complex carbohydrates, but also in detoxifying compounds and mitigating the impacts of inhospitable environments. Further research is needed to fully understand the intricate relationships between sea urchin species, their habitats, diets, and gut microbiota composition. A deeper understanding of these interactions may inform sustainable management and aquaculture strategies, ensure the long-term viability of economically valuable sea urchin species, and provide insights into their ecological roles and adaptability to changing environments.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7363987","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight Into Dietary Utilization of African Palm Weevil (Rhynchophorus phoenicis) Oil: Assessing the Effect on Growth, Serum Biochemistry, Liver, and Intestinal Histomorphology of African Catfish (Clarias gariepinus)","authors":"Femi J. Fawole,&nbsp;Yusuf O. Yusuf,&nbsp;Lateef O. Tiamiyu,&nbsp;Orighomisan N. Arawore,&nbsp;Praise B. Akinlabi,&nbsp;Omoshalewa H. Jimoh,&nbsp;Kazeem D. Adeyemi,&nbsp;Olubunmi Atolani,&nbsp;Adam Abdullahi","doi":"10.1155/are/4580197","DOIUrl":"https://doi.org/10.1155/are/4580197","url":null,"abstract":"<div>\u0000 <p>The quest to reduce dependency on fish- or vegetable-based oil resources has prompted aquaculture experts to intensify their search for a suitable and sustainable alternative for aquafeed production. Based on this reality, the current study was designed to evaluate the dietary influence of African palm weevil oil (PWO) on the growth performance, serum biochemistry, lipid peroxidation products, antioxidant enzyme response, and organ integrity of African catfish (<i>Clarias gariepinus</i>) juveniles. Four diets were formulated, wherein the control diet was prepared using soybean oil (SO) as the main dietary oil source and was replaced with PWO in the treatment diets at 50% (PWO-50), 75% (PWO-75), or 100% (PWO-100). A total of 180 African catfish (average weight: 18.76 ± 0.043) were randomly stocked at 15 fish per 50-L tank in triplicate and hand-fed to apparent satiation twice daily for 45 days. The results showed that the highest weight gain (WG), specific growth rate (SGR), and thermal growth coefficient (TGC) were found in control (SO)-fed fish, which is similar to the PWO 50% but differs significantly from others (<i>p</i> &lt; 0.05). The feed conversion ratio (FCR) and protein productive value (PPV) showed linear and quadratic trends, with a significantly lowest and highest value recorded in fish-fed control and PWO 50%, respectively, compared to other dietary groups (<i>p</i> &lt; 0.05). The protein efficiency ratio (PER) value decreases linearly (<i>p</i> &lt; 0.001) as the level of PWO increases. The highest total immunoglobulin (TIG) and superoxide dismutase (SOD) activity were recorded in fish fed PWO-50%, while PWO-75% registered the highest lysozyme activity (LA). There was no statistically significant difference (<i>p</i> &gt; 0.05) in the physiological parameters such as total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), C-reactive protein (CRP), and lipid peroxidation index (malondialdehyde [MDA]) among the groups. The fish fed PWO-100% had the lowest recorded values of villus height, area of absorption, and cryptal depth (<i>p</i> &lt; 0.05). In summary, the current finding shows that oil extracted from African palm weevils appears promising at a 50% dietary level without impairing the growth performance, liver, or intestinal health of African catfish.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4580197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Commercial Probiotics on the Growth, Hematology, Immunity, and Resistance to Aeromonas hydrophila Challenge in Climbing Perch, Anabas testudineus","authors":"Fardin Shahriar,&nbsp;A. H. E. Azaz Ibnasina,&nbsp;Nasib Uddin,&nbsp;Fatema Jannat Munny,&nbsp;Md Ashraf Hussain,&nbsp;Won Je Jang,&nbsp;Jong Min Lee,&nbsp;Eun-Woo Lee,&nbsp;Md Tawheed Hasan,&nbsp;Md. Abu Kawsar","doi":"10.1155/are/3901035","DOIUrl":"https://doi.org/10.1155/are/3901035","url":null,"abstract":"<div>\u0000 <p>This study investigated the effects of commercial probiotics on the growth, feed utilization, hematology, innate immunity, and disease resistance of climbing perch (<i>Anabas testudineus</i>). One gram of each locally available branded commercial probiotic, Pro-1, Pro-2, Pro-3, and Pro-4, was incorporated into commercial fish feed to prepare treatment diets T₁ to T₄, respectively, and feed without any probiotics was used as control (C). After 8 weeks of feeding, all groups demonstrated significantly improved percent weight gain (WG) and specific growth ratio (SGR; except T₃) and lower feed conversion ratio (FCR) relative to C. Moreover, these growth and feed utilization parameters were positively modulated in T₂ compared to C and the other three treatments. Serum biochemical parameters, such as liver stress enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST), were lower (<i>p</i> &lt; 0.05) in the probiotic-supplemented group, but variations in triglyceride (TG, mg dl⁻¹) levels were detected. Important hematological parameters, including white blood cells (WBC), lymphocytes (Lymphs), mean corpuscular hemoglobin concentration (MCHC), red blood cell (RBC) distribution width (RDW)-standard deviation (SD), and platelets (PLT), also improved after probiotic administration. Innate immune parameters, such as respiratory burst (RB) in T₃ and alkaline phosphatase (ALP) in T₂ to T₄, also increased. The pathogenetic bacterial challenge with <i>Aeromonas hydrophila</i> (1 × 10⁸ colony-forming units (CFUs)/mL) revealed the enhanced survival rates in T₂ and T₃ compared to other groups. Therefore, locally available commercial probiotic administration through fish diet inoculation enhanced the growth, boosted immunity and hematology, and increased infectious disease protection against <i>A. hydrophila</i> infection in <i>A. testudineus</i> to and ensured the sustainable aquaculture rearing of this species.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3901035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antiviral Effect of Two Important Base Analogs, Ribavirin and 5-Fluoruracil, on Infectious Pancreatic Necrosis Virus","authors":"Yasemin Çiçek Yıldız,&nbsp;Zafer Yazıcı","doi":"10.1155/2024/8838908","DOIUrl":"https://doi.org/10.1155/2024/8838908","url":null,"abstract":"<div>\u0000 <p>Infectious pancreatic necrosis (IPN) has highly contagious disease and an important viral disease-causing economic loss for fish farming, which has developed in recent years. The virus is the first fish virus isolated in vitro. Horizontal and vertical transmission plays an important role in the spread of the virus. In addition, the resistance of the virus to environmental conditions makes it difficult to control the virus. Therefore, it is important to develop vaccines and analyze the effectivity of antivirals for the prevention and control of infectious pancreatic necrosis virus (IPNV) infection. This study investigated the antiviral effect of two different mutagens, ribavirin (RBV) and 5-fluorouracil (5-FU), on IPNV, and their mutation-inducing potential on the susceptible cell line <i>Epithelioma papulosum cyprini</i> (EPC), in vitro. For this purpose, cytotoxic doses of RBV and 5-FU were first investigated and were found to be 50 and 100 µM/mL, respectively. Serial passages of IPNV were then performed, both with and without drug suppression. At the end of each passage, confirmation was performed by real-time transcriptase polymerase chain reaction (RT-PCR). Serial passages were checked for viral load using quantitative RT-PCR (qRT-PCR). The viral copy number of the RBV-suppressed virus decreased from 1.18 × 10⁵ copies/µL for the 1st passage to 1.49 × 10² copies/µL at the end of the 5th passage. The viral copy number of the 5-FU-suppressed virus decreased gradually with each passage, from 3.84 × 10³ copies/µL for the 1st passage to 1.78 × 10² copies/µL at the end of the 5th passage, with the exception of the 2nd passage, where the viral load increased. Passages 1st and 5th of both the RBV- and 5-FU-suppressed virus, and the non-drug-suppressed virus, were checked by partial sequence, but mutations in VP2 were not observed. In conclusion, in this study, we obtained data on the antiviral activity of two mutagens on IPNV.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8838908","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Fish and Crustacean Byproducts as Primary Ingredients in Pelleted Aquafeed: The Effect of Byproduct Type on Pellet Physical Properties","authors":"Alexander Chouljenko,&nbsp;Sanazsadat Mirtalebi,&nbsp;Stewart Hopper,&nbsp;Fernanda Santos,&nbsp;Greg Bolton","doi":"10.1155/2024/3401060","DOIUrl":"https://doi.org/10.1155/2024/3401060","url":null,"abstract":"<div>\u0000 <p>Over the past three decades, global aquaculture production has significantly increased, emphasizing the need for sustainable and cost-effective alternatives to traditional fish meal in aquafeed. This study’s objective was to elucidate the impact of utilizing a combination of fish and crustacean byproducts—namely, smoked salmon (<i>Salmo salar</i>) skins (SSs), smoked salmon trimmings (STs), and shrimp (<i>Litopenaeus setiferus</i>) heads (SHs)—as primary ingredients in pelleted aquafeed. Importantly, this work focuses on nonextruded pellets, where the physical properties are more influenced by ingredient composition compared to extruded pellets. The tested formulations were not nutritionally comprehensive for any specific commercial aquaculture species, as the goal of this study was to highlight the effect of the byproducts on pellet physical integrity. SH and de-oiled solid fractions of SS and ST were dehydrated for 24 h at 60°C, ground into dried powders, and formulated into six samples at a 1:1 (w/w) ratio and one sample at a 1:1:1 ratio. Potato starch (80 g/kg) was added to each sample, along with varying deionized (DI) water amounts before steam conditioning, pelletizing, and drying. Analyses included pellet nutrient composition, floatability, durability, water stability, bulk density, water absorption index (WAI), and water solubility index (WSI). Results revealed that SS pellets exhibited 97% floatability at 1 min, decreasing to 70% at 60 min. SS and ST combinations showed slow sinking behavior, while all other formulations sank quickly. Pellets containing SH had lower water stability (65.65% ± 4.44% to 0.05% ± 0.07%), in contrast to over 92% for SS and ST pellets. Durability ranged from 98.48% ± 0.37% for SS to 75.29% ± 5.82% for SH and ST combinations, further underlining the significant impact of byproduct choice on pellet performance. Overall, the inclusion of SS or ST and their combination as primary ingredients for pelleted aquafeed produced pellets that scored well on important quality parameters, while pellets containing SH performed poorly. This information may be used in the development of nutritionally comprehensive nonextruded aquafeeds containing SS and/or ST to conduct feeding trials with commercially relevant species.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3401060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction and Survival of Diploid Gynogenesis With Ultraviolet Light-Irradiated Sperm in the Manila Clam Ruditapes philippinarum","authors":"Qiang Li,&nbsp;Tao Liu,&nbsp;Hongda Li,&nbsp;Bin Wang,&nbsp;Hongtao Nie","doi":"10.1155/2024/5552528","DOIUrl":"https://doi.org/10.1155/2024/5552528","url":null,"abstract":"<div>\u0000 <p>In order to produce gynogenetic diploid of Manila clam, <i>Ruditapes philippinarum</i>, we studied the optimal conditions of gynogenetic diploid induced by ultraviolet (UV). The irradiance of UV-induced sperm was 230 μW (cm²·s), and the irradiation time was 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 s. After the egg fertilized with genetically inactivated sperm, the fertilized eggs were treated with cytochalasin B (CB) at different concentrations of 0, 0.25, 0.50, 0.75, and 1.0 mg/L for 20 min. The ploidy of larvae under different induction concentrations were analyzed by flow cytometry, and the chromosome division process during sperm entry and cleavage was observed by fluorescence microscope. In addition, the ultrastructure of sperm, embryonic development, and chromosome were observed. The results showed that the fertilization rate and cleavage rate decreased gradually with the prolongation of UV irradiation time. We found UV irradiation for 9 s was the optimal time for haploid formation, with an induction rate of 100%. The CB treatment at 0.75 mg/L was the optimal concentration for the formation of D-larvae, and the induction rate was 9.60% ± 5.10%. The results showed that the optimal conditions for inducing gynogenetic diploid of <i>R. philippinarum</i> were irradiation with UV intensity of 230 μW (cm²·s) for 9 s and inhibited second polar body release by CB at concentration of 0.75 mg/L. In this study, the conditions for inducing Manila clam gynogenetic diploid were obtained, which lays the basis for further genetic research on the gynogenesis of <i>R. philippinarum</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5552528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Producing Supersaturated Dissolved Oxygen Seawater by Photosynthesis of Ulva sp. and Its Applications","authors":"Shin Hirayama,&nbsp;Kazuya Urata,&nbsp;Miyuki Kusumoto,&nbsp;Yasuyuki Ikegami","doi":"10.1155/2024/9411516","DOIUrl":"https://doi.org/10.1155/2024/9411516","url":null,"abstract":"<div>\u0000 <p>In order to create new value in biomass production using sterile <i>Ulva</i> with high production efficiency, we selected sterile <i>Ulva</i> with high culture stability from the coast of Imari City in Japan and investigated the conditions for generating high concentrations of dissolved oxygen through photosynthesis using the sterile <i>Ulva</i>. In the cultivation of sterile <i>Ulva</i>, we investigated the conditions for generating high concentrations of dissolved oxygen (DO) by controlling conditions such as aeration, NO₃-N concentration, and population density. As a result, we found that under the cultivation control conditions we found, DO reached about 3.8 times the saturation concentration after 3 h. It was found that this high level of DO can be maintained if the supersaturated DO seawater obtained here is properly sealed. Seawater containing high levels of DO is essential for the cultivation of oysters and shellfish, which also prefer a growth temperature of 20°C−25°C. Therefore, from the perspective of creating new value in biomass production, we developed a new system for simultaneously cultivating sterile <i>Ulva</i> and cultivating oysters.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/9411516","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}