Aquaculture Research最新文献

{"title":"Could Biofloc Technology (BFT) Pave the Way Toward a More Sustainable Aquaculture in Line With the Circular Economy?","authors":"Maurício Gustavo Coelho Emerenciano,&nbsp;Mohammad Hossein Khanjani,&nbsp;Moslem Sharifinia,&nbsp;Anselmo Miranda-Baeza","doi":"10.1155/are/1020045","DOIUrl":"https://doi.org/10.1155/are/1020045","url":null,"abstract":"<div>\u0000 <p>Aquaculture is a growing industry, but current practices and raw material utilization must be reviewed to ensure a resilient and sustainable development. In this sense, the transition from a linear economy (take, make, dispose) to a circular one (renew, remake) is accelerating. The biofloc technology (BFT) is a relatively new cultivation system that can be adopted to accomplish more sustainable aquaculture and circularity goals. This document discusses BFT and its association with the circular economy (CE), the current aquaculture challenges, and the role of BFT in overcoming those challenges. This manuscript adopts Cramer’s 10 R’s and Muscat et al.’s five P’s frameworks to understand whether a functioning BFT and its key compartments (i.e., feed, environment, water, system, and microbials) align with CE’s core principles. In addition, the present work provides and discusses relevant insights regarding the further (industry and academia) application of CE approaches, especially in a biofloc-based farming context. According to the findings and connections with Cramer’s 10 R’s and Muscat et al.’s five P’s frameworks, BFT encompasses several transitioning steps into circularity and could play a crucial role toward a more sustainable aquaculture in line with the CE.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1020045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143117826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zooplankton in Aquaculture: A Perspective on Nutrition and Cost-Effectiveness","authors":"Najmus Sakib Khan,&nbsp;Mohammad Shamsur Rahman","doi":"10.1155/are/5347147","DOIUrl":"https://doi.org/10.1155/are/5347147","url":null,"abstract":"<div>\u0000 <p>Successful aquaculture relies on effective feeding strategies for target species, from the larval stage through to sexually mature adults, in both marine and freshwater environments. Aquatic organisms, particularly larvae, instinctively prefer live feeds like algae and zooplankton; larvae are entirely dependent on live feeds due to their undeveloped digestive systems and lack of essential digestive enzymes. Additionally, fishes and crustaceans require essential nutrients, vitamins, and minerals for sexual maturity and giving birth to healthy offspring. Zooplankton plays an important role at larval feeding stages in cost-effective aquaculture. Rotifers, cladocerans, copepods, and <i>Artemia</i> are commonly applied in larval rearing. However, naturally live feeds are deficient in valuable nutrients. Consequently, live feeds are improved with essential vitamins, minerals, and amino acids through bio-enrichments and algal diets for better performance. Aquaculturists primarily prefer zooplankton as live prey of larvae in fish and crustacean hatcheries because larvae requires essential digestive enzymes. They always give priority to artificial feeds with minimum live feeds to maximize profit by lowering the cost. Remarkable case studies are discussed on cost-effectiveness of zooplankton in sustainable aquaculture in this manuscript. Therefore, zooplankton as live feed could be a better choice for their nutritional value, palatability, natural availability, and cost-effectiveness. The notable opportunities, challenges, and advanced researches in live feed culture are also described in this current study. Artificial feed probably is a key stroke or booster tool for rapid profit, but live feeds such as zooplankton ensure the nutritional quality for sustainable aquaculture and satisfactory profit.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5347147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Potentials of Sajana (Moringa oleifera Lam.) as a Plant-Based Feed Ingredient to Sustainable and Good Aquaculture Practices: An Analysis of Growth Performance and Health Benefits","authors":"Md. Abdullah Al Mamun Hridoy,&nbsp;Fatema Jannat Munny,&nbsp;Fardin Shahriar,&nbsp;Md. Moshiur Rahman,&nbsp;Md. Fakhrul Islam,&nbsp;Abeer Kazmi,&nbsp;Md. Abu Kawsar","doi":"10.1155/are/3580123","DOIUrl":"https://doi.org/10.1155/are/3580123","url":null,"abstract":"<div>\u0000 <p>As aquaculture increasingly seeks sustainable alternatives to traditional feed ingredients such as fishmeal and fish oil, <i>Moringa oleifera</i>, an eco-friendly and widely available plant, has emerged as a promising plant-based feed ingredient. This review synthesizes research from 1988 to 2024, sourced from PubMed, Google Scholar, and Research Gate, to evaluate the nutritional and medicinal potentials of <i>M. oleifera</i> in aquaculture feeds. Rich in protein, essential amino acids, vitamins (A, C, and E), minerals (calcium, iron, and potassium), and polyunsaturated fatty acids, <i>M. oleifera</i> offers significant benefits. Its leaves, flowers, and rhizomes utilized as crude extracts or bioactive compounds promote growth, enhance immunity, and provide antimicrobial defense against parasites, fungi, bacteria, and viruses. Studies demonstrate its effectiveness in improving growth, nutrition, and hematology in aquaculture species while lowering production costs. Furthermore, its antioxidant properties attributed to phenolic and bioactive compounds, bolster fish health, and resilience. This review underscores the potential of <i>M. oleifera</i> to advance sustainable aquaculture practices through its dual nutritional and medicinal benefits.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3580123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Dietary Supplementation With Quercetin or Rutin Impact Growth, Stress, and Immunity in Pikeperch (Sander lucioperca) and the Water Microbial Community in Recirculating Aquaculture?","authors":"Paula Senff,&nbsp;Jérôme Lambert,&nbsp;Jérémy Grosjean,&nbsp;Sara Abd Al Rahim,&nbsp;Sylvain Milla,&nbsp;Christophe Robin,&nbsp;Yves Le Roux,&nbsp;Patrick Kestemont,&nbsp;Frédérique Changey,&nbsp;Pascal Fontaine","doi":"10.1155/are/2611939","DOIUrl":"https://doi.org/10.1155/are/2611939","url":null,"abstract":"<div>\u0000 <p>Pikeperch <i>Sander lucioperca</i> is a species mainly reared in recirculating aquaculture systems (RASs), but it remains very sensitive to stress and handling. Plant phenolic compounds such as quercetin or rutin are frequently investigated as feed additives to improve the growth and well-being of fish reared in RAS, but their impact on pikeperch and RAS microbial communities has never been studied. Our objectives were to (i) test the effect of quercetin or rutin addition to feed (2.2 g kg⁻¹ diet) on the growth, stress, and innate immunity of pikeperch reared in RAS and (ii) assess the consequence of those additives on the RAS water microbial communities. Pikeperch of 307 (±45) g starting weight were reared in nine experimental RAS (<i>n</i> = 3) and fed for 84 days on a diet enriched or not (control treatment) with quercetin or rutin (feeding rate: 1.5%). Considering the start (T0) and the end (T84) of the experiment, we compare the fish growth, feed conversion ratio (FCR), morphoanatomical and physiological status, and the water microbial diversity and composition through a metabarcoding approach performed on the 16S rRNA gene. Whereas no effect on fish was recorded with quercetin (624.0 ± 21.9 g final weight), rutin (558.0 ± 10.7 g final weight) reduced pikeperch growth (0.54 vs. 0.69%–0.64% day⁻¹), and increased FCR (1.70 vs. 1.2–1.4) compared to control (622.0 ± 16.8 g final weight) and quercetin treatments, respectively. No effect was observed on all fish physiological parameters analyzed separately, but a multifactorial analysis reveals that fish fed with rutin have a different overall physiological state. Quercetin induced a shift in the microbial community structure and composition, whereas there was no effect of treatments on microbial richness or evenness. A dietary supplementation with quercetin or rutin has no or a negative impact on pikeperch growth and physiology and modifies the microbial diversity in RAS. Effects of additives being species-dependent, precautions must be taken when formulating feed supplements, which must be based on precise studies.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/2611939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting Growth, Muscle Development, and Intestinal Morphology in Gangetic Mystus (Mystus cavasius) With Dietary Synbiotics","authors":"Md. Hamidul Islam,&nbsp;Sadia Salam Linda,&nbsp;Mohd Golam Quader Khan,&nbsp;M. Sadiqul Islam","doi":"10.1155/are/3638368","DOIUrl":"https://doi.org/10.1155/are/3638368","url":null,"abstract":"<div>\u0000 <p>This study examined the impact of dietary synbiotics on the growth performance, muscle proliferation, and intestinal histomorphology of Gangetic mystus (<i>Mystus cavasius</i>). Synbiotics, which combine probiotics and prebiotics, are thought to enhance fish health by improving a balanced gut microbiota. Four experimental diets were prepared with different concentrations of synbiotics (0%, 4%, 6%, and 8%) and fed to this fish over a 45-day period. Growth performance of the fish significantly improved as the level of synbiotics in their diet increased. Key metrics like final weight, weight gain, specific growth rate (SGR), and food conversion ratio all showed improvement. The diet containing 8% synbiotics resulted in the highest growth rates. A second-order polynomial regression of SGR revealed that the optimal growth occurred when the diet included ~8% of synbiotics. Histological analysis revealed that the 8% synbiotic diet led to the greatest increases in villi length, villi width, and goblet cell numbers, indicating enhanced gut health and nutrient absorption. Liver histology showed improved hepatocyte health, while muscle tissue analysis demonstrated higher muscle cell counts with higher synbiotic inclusion. These findings suggest that dietary synbiotics can effectively boost growth performance, intestinal structure, and tissue development in Gangetic mystus, offering potential benefits for aquaculture practices. Future research should explore the long-term effects and applicability of synbiotics across various fish species and environmental conditions.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3638368","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient Profiling of Some Terrestrial Plant Leaves for Their Prospective Use as Nonconventional Ingredients in Carp Diets","authors":"Dibyendu Acharya,&nbsp;Zulhisyam Abdul Kari,&nbsp;Lee Seong Wei,&nbsp;Koushik Ghosh","doi":"10.1155/are/9989338","DOIUrl":"https://doi.org/10.1155/are/9989338","url":null,"abstract":"<div>\u0000 <p>The present study was performed to investigate the basic chemical composition and nutritional value of 10 terrestrial plant leaves (viz. <i>Mangifera indica, Litchi chinensis, Artocarpus heterophyllus, Ziziphus mauritiana, Albizia lebbeck, Tamarindus indica, Cicer arietinum, Bauhinia acuminata, Delonix regia</i> and <i>Peltophorum pterocarpum</i>) abundant in tropical and subtropical environments. The leaf meals were evaluated comprehensively, encompassing proximate composition (% dry matter), amino acids, fatty acids, antinutritional factors (ANFs), antioxidants and antibacterial potential using established methodologies. The crude protein (CP) content of the leaves ranged from 10.92% to 20.74% (w/w), with the highest content observed in <i>A</i>. <i>lebbeck</i> leaves. The crude lipid (CL) and crude fibre (CF) contents varied between 1.23%–5.38% and 13.73%–24.63%, respectively (w/w). The protein-to-energy ratio (P/E) ranged between 32.32 and 60.70 mg/kcal, with significantly higher (<i>p</i>  &lt; 0.05) P/E in <i>A. lebbeck</i> compared to the other leaves. Dominant fatty acids in the evaluated plant leaves included palmitic acid (C16:0), oleic acid (C18:1n9c) and linolenic acid (C18:3n3). Considerable amounts of essential amino acids (EAAs) and nonessential amino acids (NEAAs) were recorded in the plant leaves, with maximum quantities detected in <i>A. lebbeck</i> and <i>A. heterophyllus</i>, respectively. Notably, substantial amounts of mineral elements were also detected in the plant leaves. <i>Z. mauritiana</i> was identified as a rich source of iron (Fe), copper (Cu) and manganese (Mn), while the highest calcium (Ca), phosphorus (P) and magnesium (Mg) levels were recorded in <i>A. heterophyllus</i>. Furthermore, the aqueous extracts of the leaves exhibited antioxidant and antibacterial potentials, with the maximum activities for both observed in <i>T. indica</i>. Analyses of ANFs indicated the presence of trypsin inhibitor (TI) (1.62–3.69 mg/g), tannin (21.25–61.25 mg/g), phytic acid (1.74–5.42 mg/g), cellulose (14.53%–21.37%) and hemicellulose (5.48%–10.56%) in the leaves. The study conclusively proposes the utilization of leguminous plants, particularly <i>A. lebbeck</i>, <i>B. acuminata</i> and <i>P. pterocarpum</i>, as potential nonconventional ingredients after eliminating or deactivating the major ANFs. This information on nutrients and ANFs of the less explored terrestrial plant leaves can be used to optimize low-cost carp diet formulation to ensure the economic profitability of fish farmers in the region.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9989338","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing Pregnane-3,20-Dione, 17,21-[(Methylborylene)Bis(Oxy)]-, (5. Beta)- as an Antiviral Candidate Against White Spot Syndrome Virus (WSSV): Molecular Docking and Simulation Approach","authors":"Bharath Raja,&nbsp;Abisha Sivakumar,&nbsp;Tohru Mekata,&nbsp;Vidya Radhakrishnan,&nbsp;Amel M. El Asely,&nbsp;Mohamed Abdelsalam,&nbsp;Sudhakaran Raja","doi":"10.1155/are/8855914","DOIUrl":"https://doi.org/10.1155/are/8855914","url":null,"abstract":"<div>\u0000 <p>White spot syndrome virus (WSSV) is a highly infectious virus that poses an imminent threat to global shrimp aquaculture and is responsible for significant mortality in shrimp farms. There is a lack of targeted drug therapy options for preventing or treating WSSV. Consequently, these envelope proteins have garnered attention as an alternative focus for drug development at the molecular level. In the current study, the binding efficiency of pregnane-3,20-dione, 17,21-[(methylborylene)bis(oxy)]-, (5. beta)-, a compound identified from <i>Turbianria ornata</i>, was predicted. The target proteins were major envelope proteins VP28, VP24, and VP110 of WSSV. The ligand structure was generated using PubChem and the SMILES platform. Docking was performed using AutoDock 4.2, employing blind docking to identify the preferred binding sites autonomously. A 100-nanosecond molecular dynamics (MD) simulation was conducted for each protein–ligand complex using Desmond software to evaluate the stability and dynamics of these interactions. The complexes exhibited minimum values for both docking scores and binding energies. The additional data on root mean square deviation (RMSD) and root mean square fluctuation (RMSF) further supported the stability of proteins and ligands. The compound’s strong and stable binding with multiple WSSV envelope proteins suggests its potential as a broad-spectrum antiviral agent against WSSV. The study found that the compound pregnane-3,20-dione, 17,21-[(methylborylene)bis(oxy)]-, (5. beta)- demonstrated high binding affinity <i>Turbinaria ornata</i> and stability with the viral envelope proteins VP28, VP24, and VP110, indicating strong antiviral potential against WSSV.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8855914","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143115349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Multistrain Probiotics on Growth, Hematology, Gut and Liver Morphometry, and GH and IGFs Genes Expression in Rohu (Labeo Rohita) Fry","authors":"Zannatul Ferdous,&nbsp;Fouzia Fariha,&nbsp;Nusrat Jahan,&nbsp;Sheik Istiak Md Shahriar,&nbsp;Md Kabir Hossain,&nbsp;Md Jasim Uddin,&nbsp;Md Shahjahan","doi":"10.1155/are/5892568","DOIUrl":"https://doi.org/10.1155/are/5892568","url":null,"abstract":"<div>\u0000 <p>Probiotic is regarded as an effective feed additive to increase the growth, feeding efficiency, survivability, and overall productivity of fishes in aquaculture. The following study was intended to examine the effects of multistrain probiotics on growth, blood indices, histomorphometry of gastrointestinal tract and liver, and growth hormone (GH) and insulin-like growth factors (IGFs) genes expression in rohu <i>Labeo rohita</i>. Probiotic containing <i>Bacillus subtilis</i>, <i>B. thuringiencis</i>, <i>Lactobacillus plantarum</i>, and <i>L. buchneri</i> was inoculated in water and three different doses, that is, 0 mL/L (control), 0.5 mL/L (lower dose, T1), and 1.0 mL/L (higher dose, T2) were used in this study. Each treatment had three replicates and fishes were reared in aquarium for 60 days. Results revealed that substantially higher weight gain (WG) and specific growth rate (SGR) along with least apparent feed conversion ratio (AFC) were documented in probiotic treated fish which was significantly different than that of control, however, higher dose (1.0 mL/L) showed best performance. Blood–biochemical indices such as red blood cell (RBC), white blood cell (WBC), hemoglobin (Hb), and glucose (Glu) level were observed statistically higher in probiotic additive culture water. Moreover, the inoculation of multispecies probiotic positively altered the intestinal histometry. Besides, immune response features of intestine were observed significantly higher in probiotic treated fish. The nucleus shape and distances between liver tissues (DLTs) were also improved with the upward doses of probiotic application. The mRNAs levels of GH in the pituitary and IGF-1 and IGF-2 in the liver were significantly higher in probiotic treated fish. In a nutshell, this study suggested that the inclusion of multistrain probiotics showed promising improvement in growth performance and feed conversion efficiency; additionally, uplifted the intestinal structure integrity, liver morphology, and GH and IGFs genes expression in rohu.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5892568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143114957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Algogenic Zinc Nanoparticles and Assessment of Their Biomimetics Attributes and Potential Antibacterial Efficacy Against Fish Pathogens","authors":"Eman Zahran,&nbsp;Samia Elbahnaswy,&nbsp;Manar Elsayed,&nbsp;Nehal A. Saif,&nbsp;Mohamed Elhadidy,&nbsp;Engy Risha,&nbsp;Hanan H. Abdelhafeez,&nbsp;Ferdaus Mohd Altaf Hossain,&nbsp;Abdallah Tageldein Mansour,&nbsp;Fatma Ahmed","doi":"10.1155/are/6304377","DOIUrl":"https://doi.org/10.1155/are/6304377","url":null,"abstract":"<div>\u0000 <p>This study investigates the fabrication of algogenic zinc nanoparticles (ZnNPs), examining their biomimetic properties and potential antibacterial effectiveness against fish pathogens. The rising incidence of antibiotic resistance in aquaculture demands innovative strategies for disease management, highlighting the importance of developing biocompatible and effective antimicrobial agents. In this context, ZnNPs were synthesized using <i>Pediastrum boryanum</i> microalgal extract as the reducing and capping agent. Transmission electron microscopy (TEM) revealed spherical and tetragonal particles with sizes ranging from 32.84 to 26.87 nm. Fourier transform infrared spectroscopy (FTIR) demonstrated distinctive functional groups in the algal extracts and ZnNPs. X-ray diffraction (XRD) showed pure crystallinity of the fabricated ZnNPs with an average crystalline size of 57.97 nm. The algal extract had high total phenolic (TPC) and flavonoid (TFC) contents (TPC: 53.83 and TFC: 12.44 mg QE/g, respectively). The obtained ZnNPs retained a considerable amount of TPC and TFC (7.92 and 5.31 mg/g, respectively). The microalgal extract and ZnNPs exhibited considerable antioxidant capacity in the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. In addition, they elicited antibacterial efficacy against several bacterial pathogens in fish in a minimum inhibitory concentration (MIC) assay. Brine shrimp lethality assay (BSLA) revealed higher potential cytotoxicity of biosynthesized ZnNPs at 29.28 μg/mL compared to that of the algal extracts at 51.56 μg/mL. These findings highlight the potential of <i>P. boryanum</i> as a green factory for the nanofabrication of metallic nanoparticles (NPs) and as a promising clinical candidate for fish medicine.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6304377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143114333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Calceostoma glandulosum (Monogenea) in Argyrosomus regius: Morphological and Molecular Characterization and Temperature Effects on Life Cycle","authors":"Maria Carolina Ribeiro,&nbsp;Cátia Lourenço-Marques,&nbsp;Teresa Baptista,&nbsp;Pedro Pousão-Ferreira,&nbsp;Florbela Soares","doi":"10.1155/are/9397751","DOIUrl":"https://doi.org/10.1155/are/9397751","url":null,"abstract":"<div>\u0000 <p>The increase in fish production in aquaculture reflects the growth of the world population. However, this expansion is accompanied by challenges, such as the intensification of production systems through increased stocking density, which induces stress in fish, weakening their immune system and making them more susceptible to disease outbreaks, particularly those caused by parasites. Monogeneans are ectoparasites that attach to the external surfaces of fish and are characterised by their host-specificity. One of the emerging species in aquaculture, much appreciated in the Mediterranean region, is the meagre (<i>Argyrosomus regius</i>), whose production has been increasing due to its high-quality meat and excellent growth rates. However, the information available on the parasites that affect this species is limited, which makes it difficult to prevent and control disease outbreaks in aquaculture systems effectively. This study presents the first morphological and molecular characterisation of <i>Calceostoma glandulosum</i>, an ectoparasite of the Monopisthocotylea subclass, characterised by its lapel (L) in the cephalic area and an anchor-shaped haptor (Hp). The parasite’s life cycle, including eggs, larvae (oncomiracidium), and adults, has also been described. It was demonstrated that water temperature could influence the development of the eggs; at more favourable temperatures for the parasite, there was a higher percentage of developed and hatched eggs. The occurrence of the parasite in the farming tanks was higher when the temperature reached 20°C, and in vitro experiments have shown a hatching rate of 100% at 23°C. <i>C. glandulosum</i> reaches sexual maturity between 11 and 14 days and has a fertility period of 10 days. To assess the host-specificity of <i>C. glandulosum</i> for <i>A. regius</i>, eggs were investigated in other species (<i>Sparus aurata</i>, <i>Diplodus sargus</i>, and <i>Seriola rivoliana</i>). This work provides valuable insights into the behavior of monogeneans concerning environmental conditions and host interactions, offering critical information for implementing preventive measures in aquaculture.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9397751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143113614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}