Aquaculture Research最新文献

{"title":"Exploring Silver as an Alternative Treatment for Controlling Macrogyrodactylus congolensis Populations","authors":"Lutfiyya Latief,&nbsp;Tarryn L. Botha,&nbsp;Annemariè Avenant-Oldewage","doi":"10.1155/are/8822090","DOIUrl":"https://doi.org/10.1155/are/8822090","url":null,"abstract":"<div>\u0000 <p>Monogeneans are ectoparasites that in high densities, can cause harm and even death to their hosts. Therefore, treatment methods are continuously evaluated. The current study aims to determine the LC50 of ionic silver and silver-engineered nanomaterials (ENMs) (nAg) in the monogenean <i>Macrogyrodactylus congolensis</i> and report on the toxicological effects of silver on the parasite by using their behavioural response as an indicator. <i>Macrogyrodactylus congolensis</i> were exposed in vivo to a range of ionic Ag and nAg concentrations for 12 h in three water media (reverse osmosis [RO], borehole, and aged tap water). The LC10, LC20, and LC50 were determined using ToxRat Professional. Behaviour was assessed using low, medium, and high concentrations (calculated from the parasites LC50) of ionic silver and nAg in the different water media. Videos were recorded with a camera for 5-min periods at 0, 1, 3, 6, and 12 h intervals. The videos were analysed using Noldus EthoVision XT software. The results demonstrated significant increases in the acceleration of movement, body contact between parasites, the distance travelled, mobility, and swimming speed when exposed to increased concentrations of silver. Changes that have been observed are most likely in response to toxicological stress and neurological damage caused by silver. Furthermore, silver and RO water being the most toxic, and aged tap water is the most optimal water medium. Furthermore, parasites showed an increase in behavioural changes as exposure concentrations increased. Lower LCx values were recorded when parasites were exposed to the ionic Ag compared to the nAg in all water media, indicating that the ionic silver was more toxic to <i>M. congolensis</i> than nAg, which highlights the need for the safe development of nanotechnology.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8822090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Residues and Heavy Metals in Aquaculture Farms Within Nairobi County, Kenya","authors":"Elvis M. Waga,&nbsp;Gabriel O. Aboge,&nbsp;Nduhiu Gitahi,&nbsp;Claire Heffernan,&nbsp;Joseph G. Nderitu,&nbsp;Lorna Benton","doi":"10.1155/are/9275802","DOIUrl":"https://doi.org/10.1155/are/9275802","url":null,"abstract":"<div>\u0000 <p>Inappropriate use of antibiotics in aquaculture, livestock, and agriculture contributes significantly in the development of antimicrobial resistance. This study assessed antimicrobial residues and heavy metals in fish, water, and sediments within Nairobi County, Kenya. Eighteen (18) fish farms were visited and water (17), fish (11), and sediment (9) samples were collected. The samples were analyzed using liquid chromatography coupled with tandem double mass spectrometry (LC-MS/MS) and ICP-MS for antimicrobial residues and heavy metals, respectively. Thirteen (13) antimicrobial residues: trimethoprim, sulfadiazine, sulfamethoxazole, sulfamethazine, tetracycline, doxycycline, oxytetracycline, erythromycin, azithromycin, ampicillin, penicillin G, amoxicillin, and cloxacillin tested were detected in all three sample types at concentrations below the recommended maximum residue levels. The overall percentage of tetracylines, sulfonamides, trimethoprim, beta-lactams, and macrolides antibiotic residues classes positive in water, fish, and sediment samples were (94.12%, 100%, 100%), (94.12%, 100%, 100%), (100%, 100%, 100%), (35.29%, 9.09%, 33.33%), and (94.12%, 0%, 11.11%), respectively. The mean tetracylines, sulfonamides, trimethoprim, beta-lactams, and macrolides antibiotic residues classes concentrations in water, fish, and sediment samples were (2.38 ± 5.687, 5.04 ± 2.116, 17.81 ± 21.782 ppb), (0.19 ± 0.124, 1.83 ± 3.641, 3.26 ± 1.434 ppb), (0.68 ± 0.503, 1.80 ± 1.679, 14.46 ± 10.638 ppb), (0.25 ± 0.263, 1.80 ± 1.679, 2.26 ± 2.25 ppb), and (0.76 ± 0.719 ppb, &lt;LOD, 0.05 ± 0 ppb), respectively. Heavy metals mean concentrations for chromium (Cr), lead (Pb), arsenic (As), and cadmium (Cd) in water, fish, and sediment samples (0.05 ± 0.08, 0.73 ± 0.60, 2.69 ± 1.06 mg/kg), (0.003 ± 0.002, 0.14 ± 0.11 , 3.51 ± 2.61 mg/kg), (&lt;LOD, 0.001 ± 0, 0.45 ± 0.17 mg/kg), and (&lt;LOD, &lt;LOD, 0.02 ± 0.02 mg/kg). Mercury (Hg) tested negative for all the samples analyzed. All fish samples tested had Cr concentrations exceeding the set maximum residue limit (MRL) of 0.05 mg/kg in fish muscle according to European Commission regulation 2016. Sediments recorded the highest concentrations, followed by fish and water, for both heavy metals and antimicrobial residues. Levels of heavy metals detected in fish were not associated with noncarcinogenic risks. However, Pb and Cr levels detected in the fish tissues were noted to pose carcinogenic health risks and, therefore, threatens the public health safety of fish consumption in this area of study.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9275802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report on Vibrio harveyi Infection in Black Rockfish (Sebastes schlegeli): Skin Ulcers, Histopathology, and Immune Response","authors":"Yindong Gu,&nbsp;Yongxiang Yu,&nbsp;Chunyuan Wang,&nbsp;Yingeng Wang,&nbsp;Zhiqi Zhang,&nbsp;Xiaojun Rong,&nbsp;Meijie Liao,&nbsp;Bin Li,&nbsp;Lei Qin,&nbsp;Zheng Zhang","doi":"10.1155/are/1390685","DOIUrl":"https://doi.org/10.1155/are/1390685","url":null,"abstract":"<div>\u0000 <p>In August 2023, a severe skin ulcer outbreak occurred in net-caged black rockfish (<i>Sebastes schlegeli</i>) of Nanhuangcheng Island, Bohai Sea. The causative bacterium, <i>Vibrio harveyi</i>, was identified through 16S rDNA and gyrB sequencing. Artificial infection experiments confirmed its high pathogenicity with a 7-day LD50 of 5.41 × 10⁶ CFU/mL. Histopathological analysis revealed hepatocyte vacuolization, erythrocyte aggregation in the spleen leading to splenic congestion and reducing the contrast between red and white pulp regions, glomerular atrophy, renal damage, intestinal epithelial necrosis, and muscle fiber disintegration. Immunoenzymatic assays showed elevated alkaline phosphatase (AKP), acid phosphatase (ACP), and lysozyme (LZM) activity. Additionally, qRT-PCR analysis revealed downregulation of IL-12B and upregulation of TNF-<i>α</i>, CASP10, and RAFEA. Antibiotic susceptibility testing showed sensitivity to minocycline and doxycycline, but resistance to erythromycin and azithromycin. This study provides novel etiological and histopathological insights into <i>V. harveyi</i> infection in black rockfish, highlighting the bacterium’s impact on host tissues and immune response.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1390685","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Stocking Density on Water Quality, Survival and Growth Performance of Free-Swimming Larvae of Goldfish, Carassius auratus (Linnaeus, 1758) in an Indoor Biofloc System","authors":"Vaibhav R. Gote,&nbsp;M. J. Gitte,&nbsp;S. J. Meshram,&nbsp;R. M. Tibile,&nbsp;H. B. Dhamagaye,&nbsp;Chandni Roy Dutt,&nbsp;Sarang N. Chavan,&nbsp;Shivani S. Kondekar,&nbsp;Ediga Arun Goud,&nbsp;D. R. Palwe,&nbsp;Mrunal N. Nasre","doi":"10.1155/are/8066222","DOIUrl":"https://doi.org/10.1155/are/8066222","url":null,"abstract":"<div>\u0000 <p>A 30-day experiment was conducted to assess the influence of varying stocking densities (2, 4, 6 and 8 larvae L⁻¹) on the growth performance and survival of free-swimming larvae of <i>Carassius auratus</i>, commonly known as goldfish, within an indoor biofloc system. The larvae (7.05 ± 0.02 mm in length and 1.40 ± 0.00 mg in weight) were stocked in circular tanks wherein a volume of 50 L of water was maintained and provided with vigorous aeration. The experiment used a completely randomised design with five replicates for each density group. A diet comprising a 1:1 mixture of powdered groundnut oil cake and rice bran (RB) was provided as feed. The RB was added daily as a carbon source to maintain the C/N ratio at 20:1. The highest % length gain (209.46 ± 1.47), % weight gain (14028 ± 99.65), specific growth rate (SGR) (16.50 ± 0.02), survival (85.00% ± 1.00) and the lowest apparent feed conversion ratio (AFCR) (1.84 ± 0.01) were recorded in the density group of 2 larvae L⁻¹ (<i>p</i> &lt; 0.05) as compared to other density groups. The results indicated a better stocking density of 2 larvae L⁻¹ for growing free-swimming larvae of goldfish for 30 days.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8066222","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conversion of Shrimp Aquaculture Waste Into Nutritious Floc Using Taro Flour With Varied C/N Ratios: A Sustainable Approach for Aquafeed Production","authors":"Muhamad Amin,&nbsp;Denya Safa Fitri Syahira,&nbsp;Akhmad Taufiq Mukti","doi":"10.1155/are/9924354","DOIUrl":"https://doi.org/10.1155/are/9924354","url":null,"abstract":"<div>\u0000 <p>Waste management is a critical challenge in the aquaculture industry, particularly in the cultivation of Pacific white shrimp (<i>Penaeus vannamei</i>). This study explores the potential of converting shrimp aquaculture waste into a nutritious floc biomass by incorporating taro flour as a carbon source at varying carbon-to-nitrogen (C/N) ratios. A completely randomized design was employed, with treatments consisting of five different C/N ratios (control, 12, 15, 18, and 21) and four replicates per treatment. The results revealed that the different C/N ratios significantly influenced floc formation time, floc volume, floc biomass, floc size, and the floc’s crude protein content (<i>p</i> &lt; 0.05). The treatment with the highest C/N ratio (21) demonstrated the most efficient floc formation, achieving a floc volume of 247.5 ml/L, a biomass of 34.21 g/L, and a floc size of 339 µm within 4 days. Furthermore, the crude protein content in the floc increased from 18.37% in the waste to 39.75%. The floc composition predominantly included <i>Bacillus</i> spp., <i>Chlorella</i> sp., and <i>Alona</i> sp. across all treatments, while at the highest a C/N ratio (T4), unique featured microorganisms including <i>Melosira</i> sp., <i>Paramecium</i> sp., and <i>Chroococcus</i> sp. were observed. These findings suggest that shrimp aquaculture waste can be effectively transformed into a highly nutritious material, with the potential to be developed as a sustainable protein source for aquafeed.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9924354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm Quality, Seminal Characteristics, and Their Relationship in the Bulatmai Barbel (Luciobarbus capito) Following First and Second Stripping During the Breeding Season","authors":"Erfan Akbari Nargesi,&nbsp;Danial Gorouhi","doi":"10.1155/are/9981404","DOIUrl":"https://doi.org/10.1155/are/9981404","url":null,"abstract":"<div>\u0000 <p>The present study investigated the effects of multiple sperm collections on sperm quality, seminal plasma ionic and biochemical parameters, and the relationship between measured variables in the Bulatmai barbel (<i>Luciobarbus capito</i>) during the breeding season. For this purpose, the sperm of males (<i>n</i> = 10) with a mean body weight of 1761.5 ± 385.3 g and an average total length of 55.6 ± 2.8 cm were collected through the first and second stripping process at a 1-week interval. According to the results, sperm quality parameters, including sperm volume (SV), spermatocrit, spermatozoa concentration, total sperm production (TSP), and total motility (%) significantly decreased at the second sperm sampling (<i>p</i> &lt; 0.05). The seminal plasma from the first sperm sampling had higher cholesterol and sodium levels (<i>p</i> &lt; 0.05). Besides, the osmolality of the first stripping was notably higher than comparison of the second stripping (<i>p</i> &lt; 0.05). The first and second strippings revealed significant correlations among SV–TSP, spermatocrit–TSP, spermatocrit–spermatozoa concentration, and TSP–spermatozoa concentration. Unique correlations include motility percentage–motility duration (MD), glucose-calcium (negative), glucose–MD, cholesterol–potassium, and triglyceride–magnesium in the first stripping and SV–MD, SV–spermatozoa concentration, spermatocrit–MD, TSP–MD, and total protein-osmolality in the second. The results of this study recommend that a single stripping method could be more suitable for controlled reproduction programs of Bulatmai barbel males at the hatchery centers to ensure optimal sperm viability and enhance reproductive success.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9981404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hazard Index of Microplastics Contamination in Commercial Marine Fish Species and Mussels in the Southern Marmara Sea, Turkey","authors":"Saadet Hacısalihoğlu","doi":"10.1155/are/6690338","DOIUrl":"https://doi.org/10.1155/are/6690338","url":null,"abstract":"<div>\u0000 <p>Microplastic (MP) pollution is an increasing concern in marine ecosystems due to the risk it poses to marine organisms and human health. This study aims to investigate the type, abundance, and properties of MPs in five commercially important fish species (<i>Engraulis encrasicolus</i>, <i>Trachurus trachurus</i>, <i>Sardina pilchardus</i>, <i>Sarda sarda</i>, and <i>Dicentrarchus labrax</i>) and Mediterranean mussels (<i>Mytilus galloprovincialis</i>). In the study, samples were collected from five different regions in the Southern Marmara Sea of Turkey. In addition, the polymer composition of MPs identified in the study was determined and evaluated according to the associated hazard index to determine the potential risks to marine life and human consumers. A total of 1734 MPs were identified in the gastrointestinal tracts (GITs) of fish samples (<i>n</i> = 660), while 650 MPs were identified in mussel samples (<i>n</i> = 50). The analyses revealed that there was significant interspecific and spatial variability in MP abundance, size, shape, and color. The most common MPs are those smaller than 0.3 mm in diameter. These are predominantly in the form of lines and beads in all species. Fourier transform infrared (FTIR) spectroscopy was used to identify the types of polymers present. The types of polymers identified in the study were polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polyethylene terephthalate (PET), and ethylene vinyl acetate (EVA). The most common polymers were PE and PP, which showed hazard indices classified as “hazard” and “high risk,” respectively. The study identifies potential health risks associated with MPs, particularly in marine organisms, including apex predators such as <i>D. labrax</i> and filter feeders such as mussels. These findings highlight the need for effective waste management strategies, stricter regulations, and increased public awareness to reduce pollution of marine environments with plastic.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6690338","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case Study Documenting Growth and Welfare of Atlantic Salmon Reared to Market Size in a Novel Commercial Hybrid Flow-Through System in Norway: A Comparison With Fish Performance in Traditional Sea Sites","authors":"Enrique Pino-Martinez,&nbsp;Sigurd O. Handeland","doi":"10.1155/are/4018594","DOIUrl":"https://doi.org/10.1155/are/4018594","url":null,"abstract":"<div>\u0000 <p>Production of harvest-size Atlantic salmon in land-based hybrid flow-through systems (HFS) has recently emerged as an alternative to overcome sustainability challenges linked to both the production of postsmolts in recirculation aquaculture systems (RAS) and the grow-out phase at sea. In the present case study, we assessed growth, stress and welfare, key performance indicators (KPIs), and harvest results in a commercial seawater HFS facility, where Atlantic salmon were reared from smolt (~92 g) to market size (~5 kg) between June 2023 and May/June 2024. This was compared with the performance of salmon transferred from the HFS to open net pens at 910 g (to Site A) and 1900 g (to Site B). Salmon in HFS displayed better growth (over 80% higher body weight in May 2024), welfare and stress indicators (lower incidence of skin ulcers, fin damage, and lower plasma cortisol), better KPIs of growth, feeding efficiency and mortality, and better harvest results than in traditional sea Sites A and B. This positive effect could be linked to the absence of severe disturbances and stressful operations in HFS (i.e., well-boat transport or de-lousings) and to the provision of a high-quality rearing environment with large amounts of new fresh seawater, but isolated from external stressors like parasites or pathogens. HFS, thus, appears as a plausible alternative to produce fast-growing harvest-size salmon with high welfare standards, which may raise the interest of more salmon producers.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4018594","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity-Induced Gene Expressions in Pangasius nasutus (Bleeker, 1863)","authors":"Mohamad Ali Nabilah,&nbsp;Victor Tosin Okomoda,&nbsp;Danish Daniel Abdullah Muhd,&nbsp;Asma Ariffin Nur,&nbsp;Sheriff Md. Shahreza","doi":"10.1155/are/4058733","DOIUrl":"https://doi.org/10.1155/are/4058733","url":null,"abstract":"<div>\u0000 <p>This study was designed to evaluate the molecular responses of <i>Pangasius nasutus</i> to salinity exposure of varying concentrations. Juveniles of <i>P. nasutus</i> from similar breeding history (average size of 35 ± 0.54 cm and 152 ± 1.82 g) were obtained from a known source in Terengganu. The salinity range used for this study (i.e., 0, 10, 15, and 20 ppt) was determined through a range finding test, and the juveniles of <i>P. nasutus</i> were exposed in triplicates to these selected salinity ranges for 2 weeks under laboratory conditions. Thereafter, muscle tissues were collected from 10 biological replicates for molecular analysis. Differential display reverse transcriptase PCR was used to identify the expressed cDNA fragments, while real-time PCR was used to determine the regulation of selected genes. The results showed that the 14 differentially expressed genes (DEGs) identified were homologous to nine known genes in GenBank. These proteins include <i>fructose biphosphate aldolase A (ALDOA)</i>, <i>troponin I (TnI)</i>, <i>myo</i>sin <i>heavy chain (MYH)</i>, <i>myo</i>sin <i>light chain 1a(MLC 1a)</i>, <i>creatine kinase (CK)</i>, <i>ATPase ii subunit 8 (ATP8) and 6 (ATP6)</i>, <i>parvalbumin(PV)</i>, <i>ribosomal protein L26(RPL26)</i>, and <i>L11 (RPL11)</i>. Real-time quantitative PCR (qPCR) analysis of <i>TnI</i>, <i>MYH</i>, and <i>PV</i> showed that the highest expression (p  &lt; 0.05) occurred in the 20 ppt salinity treatment group, i.e., 8.79, 3.76, and 1.79 fold more induction than in the control group, respectively. However, the expression of the growth hormone (GH) gene was higher in the 10 ppt salinity treatment than in the control group (p  &lt; 0.05). This study confirmed that salinity exposure induces the expression of several genes in <i>P. nasutus</i>, however, future studies need to unravel the importance of these genes for fish performance.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4058733","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Chlorpyriphos as an Organophosphate Pesticide on Hematology, Blood Biochemistry, Antioxidant Capacity, Oxidative Stress, and Histopathological Changes in Juvenile Silver Barb (Barbonymus gonionotus)","authors":"Zakir Hossain,&nbsp;Rabeya Akter,&nbsp;Maria Binte Moin,&nbsp;Shaon Kumar Mondol,&nbsp;Tutul Kumar Saha,&nbsp;Israt Jahan Tumpa,&nbsp;Anika Tabassum,&nbsp;Sadia Ibnat,&nbsp;Mst. Arzu Pervin,&nbsp;Halima Jahan,&nbsp;Taesun Min,&nbsp;Mohammad Moniruzzaman","doi":"10.1155/are/7426390","DOIUrl":"https://doi.org/10.1155/are/7426390","url":null,"abstract":"<div>\u0000 <p>The freshwater fish population in Bangladesh is adversely affected by the use of pesticides in agriculture. Furthermore, pesticide consumption is increasing daily due to the introduction of high-yielding crop varieties. This study aimed to examine the impact of the organophosphate pesticide chlorpyriphos on various pathological alterations and acute toxicity tests in juvenile silver barb (<i>Barbonymus gonionotus</i>). This was achieved by elucidating multiple biomarkers in a time- and dose-dependent manner. Treatment of the fish with chlorpyriphos at a sublethal dose (0.005 ppm) compared to the control group led to changes in the hematopoietic parameters, organ-specific histo-morphologies, and enzymatic activities in fish. Among the notable abnormalities were gill clubbing, missing gill lamellae, hyperplasia, nuclear hypertrophy, vacuolation, glomerular expansion, increased renal tubular width, hemorrhage, necrosis, and pyknosis in fish. Large lymphocytes, dead cells, fused cells, binucleated cells, tear-shaped cells, ghost cells, senile cells, and aberrant cell shapes in peripheral nuclear erythrocytes were also observed. Impact of chlorpyriphos in silver barb exhibited a significant decrease in the parameters such as hemoglobin (g/dL), hematocrit/packed cell volume (PCV) (%), red blood cell (× 10⁶ mm³), white blood cell (× 10³/mm³), mean corpuscular hemoglobin concentration (%), and blood glucose level (nmol/L). However, the mean cell volume (µm³) and mean cell hemoglobin (pg) counts exhibited an opposite order. Furthermore, fish exposed to chlorpyriphos enhanced the serum glucose, glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), indicating liver toxicity in fish; however, brain acetylcholinesterase enzyme activity was significantly reduced than in the control, indicating neurodegeneration in fish. The superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione S-transferase (GST) were examined as critical components of the antioxidant defense system in a time-dependent manner relative to the control group. It can be stated that organophosphate pesticides produce oxidative stress, resulting in the generation of free radicals and alterations in antioxidants, oxygen free radicals, the scavenging enzyme system, and lipid peroxidation. Overall, the results demonstrated that the chlorpyriphos pesticide negatively affected the enzymatic activities, hematology, and organ-specific histology in juvenile silver barb.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7426390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}