Aquaculture Research最新文献

{"title":"Exploring the Coexposure Effects of Pyrogallol and Microplastic on the Red Swamp Crayfish Procambarus clarkii","authors":"Rashad E. M. Said,&nbsp;Mohamed Hamed,&nbsp;Walaa M. Shaalan,&nbsp;Heba Allah M. Elbaghdady,&nbsp;Alaa El-Din H. Sayed","doi":"10.1155/are/6084150","DOIUrl":"https://doi.org/10.1155/are/6084150","url":null,"abstract":"<div>\u0000 <p>Investigating the impact of two or more toxic chemicals on aquatic organisms is one of the main objectives of toxicological research. Microplastics (MPs) are identified as a carrier or vector for other pollutants. Therefore, the current study investigated the effects of both pyrogallol (PG) and MPs, individually and in combination, on freshwater crayfish (<i>Procambarus clarkii</i>). Following a 15-day exposure to 10 mg/L of PG and100 mg/L MPs and their combination (10 mg/L PG + 100 mg/L MPs), the hemocyte count, hepatopancreatic parameters, antioxidant variables, and histopathological markers were all assessed in the crayfish. The results revealed that PG and MPs potentially individually or combined altered the hemocyte count (including granular and semigranular) compared to control group (0.001 ≥ <i>p</i> &lt; 0.0001). The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and protein showed significant elevation (<i>p</i> &lt; 0.05) in PG–MP-exposed fish compared to control ones. In comparison to control <i>P. clarkii</i>, the measured oxidation indicators, superoxide dismutase (SOD), glutathione (GSH), and total antioxidant capacity (TAC), showed a significant reduction after single exposure to PG and MPs (0.001 &gt; <i>p</i> &lt; 0.0001), while catalase (CAT) and malondialdehyde (MDA) elevated drastically (0.05 &gt; <i>p</i> &lt; 0.0001). The histology of the hepatopancreas has shown many deformities and abnormalities after individual or combined toxicity, such as vacuolations, degraded hepatopancreatic tubules, eosinophilic deposits, hemocytic infiltrations, and aberrant tubules. Accordingly, there is a synergistic relationship between PG and MP toxicity to crayfish. Therefore, it is crucial to routinely check and assess the discharge of waste into waterways.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6084150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Protective Immunological Effects of Pandanus tectorius Leaf Extract Against Pathogenic Vibrio campbellii Using Gnotobiotic Brine Shrimp Model System","authors":"Patabandi PPSK,&nbsp;Mohamad Asmidar,&nbsp;Yosie Andriani,&nbsp;Parisa Norouzitallab,&nbsp;Yeong Yik Sung,&nbsp;Kartik Baruah","doi":"10.1155/are/8215825","DOIUrl":"https://doi.org/10.1155/are/8215825","url":null,"abstract":"<div>\u0000 <p>This study investigated the protective effects of <i>Pandanus tectorius</i> leaf extract (PLE) on the tolerance of the brine shrimp <i>Artemia franciscana</i> against the pathogen <i>Vibrio campbellii</i>. Axenic <i>Artemia</i> nauplii were hatched and exposed to PLE for 2 h, after which their resistance to <i>V. campbellii</i> was examined. Protective responses were evaluated by measuring nauplii survival and changes in the expression of key immune-related genes, such as heat shock protein 70 (<i>hsp70</i>), <i>hsp60</i>, <i>hsp90</i>, high mobility group box 1 (<i>hmgb1</i>), prophenoloxidase (<i>proPO</i>), and transglutaminase (<i>tgase</i>) upon <i>V. campbellii</i> challenge. Additionally, a <i>Vibrio</i> colonization assay was conducted to assess PLE’s antimicrobial potential. The results revealed that 2 h pretreatment with PLE at a concentration of 1 g/L significantly improved survival against <i>V. campbellii</i>. Immune-related gene expression was induced at different time points (e.g., 12–24 h) during the <i>Vibrio</i> challenge and the colonization assay confirmed PLE’s antimicrobial properties. The observed protection could likely be due to a combination of immune gene activation, antioxidant activity, and the antimicrobial effect of the extract. This study highlights the significance of PLE by indicating its potential to serve as a protective agent in aquaculture, enhancing the resilience of aquatic organisms against biotic stressors like vibrios.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8215825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and Morphology Analysis Among the F1 Hybrid Chinese Soft-Shelled Turtle (Pelodiscus sinensis Huangsha ♂×Pelodiscus sinensis Japanese Strains ♀) and the Parental Varieties","authors":"Yixin Liang,&nbsp;Pei Wang,&nbsp;Hewei Xiao,&nbsp;Lizhong Jin,&nbsp;Changqing Huang,&nbsp;Qin Qin,&nbsp;Shuting Xiong,&nbsp;Fanjian Meng,&nbsp;Xiaoqing Wang,&nbsp;Yazhou Hu","doi":"10.1155/are/7076743","DOIUrl":"https://doi.org/10.1155/are/7076743","url":null,"abstract":"<div>\u0000 <p>Hybridization is an effective method for the genetic improvement of breeding varieties, while establishing standardized management systems and identification methods is an important foundation for obtaining stable hybrid new varieties with desirable traits. This study conducted measurements and analyses of eight morphological indicators of three Chinese soft-shelled turtle (<i>Pelodiscus sinensis</i>) populations: the Huangsha turtle (<i>P. sinensis</i> Huangsha, HS), Japanese turtle (<i>P</i>. <i>sinensis</i> Japanese strains, JP), and their hybrid F1 generation (HS ♂ × JP ♀, HJ). Based on several important indicators, a discriminant function was established, and after cross-validation, the comprehensive discriminant accuracy was 86.4%. In addition, we used super-genotyping-by-sequencing (GBS) sequencing technology to analyze the genetic structure and genetic diversity of the three populations. The results showed that the HJ population had a closer genetic relationship with the JP population as the maternal parent and a farther genetic relationship with the HS population. The genetic diversity was between the HS and JP populations, and the genetic distance between the HS and JP populations was far. Through fingerprint analysis technology, core SNP markers for germplasm identification were screened, and a total of 148 core SNP marker loci were obtained. One SNP marker that could distinguish the HJ population from the HS population was developed. This study will provide reference for the hybrid breeding and genetic management of Chinese soft-shelled turtle.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7076743","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm Quality, Milt Biochemistry, and Cellular Ultrastructure in Fresh and Cryopreserved Sperm of Brycon amazonicus","authors":"Owens José Barros-Barrios,&nbsp;Roger Oswaldo Suárez-Martínez,&nbsp;Edwin Gómez-Ramírez,&nbsp;Diana Nathalie Guaje-Ramírez,&nbsp;Víctor Mauricio Medina-Robles","doi":"10.1155/are/6671651","DOIUrl":"https://doi.org/10.1155/are/6671651","url":null,"abstract":"<div>\u0000 <p>Milt cryopreservation facilitates native fish’s constant production of commercial interest. However, it can damage spermatozoa (sptz) due to the osmotic stress of diluents and low temperatures. This study compares spermatic quality in fresh (SF) and cryopreserved (CS) milt in <i>B. amazonicus</i> for 24 h, 1 month, and 3 months. Eight sexually mature males were induced with carp hypophysis extract. Milt was diluted in one proportion in a glucose solution (5.5%), egg yolk (12%), and dimethyl sulfoxide (10%). An evaluation of parameters such as motility, viability, morphology, total antioxidant capacity (TAC), and adenosine triphosphate (ATP) contents was performed at 24 h, 1 month, and 3 months, and the fertility test was conducted at 24 months of cryopreservation. The motility percentage was higher (<i>p</i> ≤ 0.05) in SF (95.5 ± 2.9%). Motility duration of SF was significantly higher (113.6 ± 22.6 s) than that of CS at 3 months (71 ± 12.2 s). The spermatic viability of SF was higher (97.1 ± 1%) in comparison with CS treatments. Sperm anomalies in SF were lower (12.4 ± 3.2%) than in all other treatments. ATP contents of (1.74 ± 4.5 nM × 10⁸ sptz) were superior only (<i>p</i> ≤ 0.05) in CS of 1 month (1.16 ± 1.2 nM × 10⁸ sptz). TAC was higher (<i>p</i> ≤ 0.05) in SF (26.46 ± 1.2 mM of Trolox [6-hydroxy-2,5,7,8-tetramethilcromane-acid 2-carboxylic]) compared to other CS treatments. However, there were not any significant differences in this parameter between cryopreservation times (<i>p</i> ≤ 0.05). Fertility rates were the same for all treatments. In terms of cellular ultrastructure, it was observed that <i>B. amazonicus</i> sptz had an oval-shaped head without an acrosome, middle part, and only one flagellum.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6671651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Body Weight, Temperature, and Daily Time Periods on Oxygen Consumption Rate of Juvenile Silver Pomfret (Pampus argenteus)","authors":"Yu Liu,&nbsp;Minhai Liu,&nbsp;Jieyi Chen,&nbsp;Daning Jiang,&nbsp;Jingjie Hu,&nbsp;Zhihua Lin","doi":"10.1155/are/1513694","DOIUrl":"https://doi.org/10.1155/are/1513694","url":null,"abstract":"<div>\u0000 <p>Oxygen consumption rate (OCR), which reflects the metabolism requirement of fish, is regulated by body weight and temperature. However, the priority of body weight and temperature in determining the OCR of fish is not clear. The optimal temperature and feeding time for the commercially important silver pomfret (<i>Pampus argenteus</i>) in China were evaluated using two sizes of fish (15.8 ± 2.7 g, S; 35.3 ± 6.1 g, L) at four different water temperatures (21, 24, 27, 30°C) and four time periods (5:00–6:00, 11:00–12:00, 17:00–18:00, 23:00–24:00) in static respirometers. The results showed that the OCR of fish was significantly higher at 21 and 24°C than that at 27 and 30°C (<i>p</i> &lt; 0.05). The <i>Q</i>₁₀ increased from range of 21 and 24°C to range of 24–27°C but decreased from range of 24–27°C to range of 27–30°C. The body weight and water temperature significantly affected the OCR of fish (<i>p</i> &lt; 0.05). The OCR of S was significantly higher than that of L (<i>p</i> &lt; 0.05), while the OCR of fish increased linearly with increasing temperature. The OCR decreased with increasing fish body weight, and the coefficient (<i>a</i> – 1) in the linear regression equation of the logarithmic plot ranged from −0.568 to −0.191 (11.2–47.4 g, 21–30°C), with the mean value was −0.346. Overall, a two-factor regression equation for OCR of silver pomfret was established as OCR = 0.0558 × <i>W –</i>0.0081 × <i>T</i> + 0.0341 (<i>N</i> = 120, <i>R</i>² = 0.5626, <i>p</i> &lt; 0.05). The OCR was relatively higher at 5:00–6:00 compared to other time periods of the day. These results suggested that body weight exerted a more significant influence on the metabolic rate of silver pomfret compared to temperature. The optimal temperature for this fish species was between 27 and 30°C based on <i>Q</i>₁₀, and the recommended feeding time was at 5:00–6:00 for maximizing growth for silver pomfret.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1513694","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Exogenous Dietary Protease and a Humic Substance on Growth and Microbial Composition of Nile Tilapia (Oreochromis niloticus) and Romaine Lettuce (Lactuca sativa) in a Biofloc-Integrated Decoupled Aquaponics System","authors":"Uthpala M. Padeniya,&nbsp;Dorcas Lukwesa,&nbsp;Jacob W. Bledsoe,&nbsp;D. Allen Davis,&nbsp;Daniel E. Wells,&nbsp;Benjamin H. Beck,&nbsp;Anita M. Kelly,&nbsp;Luke A. Roy,&nbsp;Timothy J. Bruce","doi":"10.1155/are/4062437","DOIUrl":"https://doi.org/10.1155/are/4062437","url":null,"abstract":"<div>\u0000 <p>Aquaponics and biofloc-based aquaculture systems are environmentally sustainable food production systems. When biofloc technology is combined with hydroponic systems, it expands economic diversity by producing additional value-added products. Dietary additives were incorporated into fish feeds in this trial to improve food production in decoupled aquaponic systems. In this decoupled aquaponic system, the biofloc aquaculture system consisted of nine cylindrical tanks of 3750 L with 170 juvenile Nile tilapia (9.99 ± 0.54 g) stocked in each tank. The fish were randomly assigned to one of three treatment groups: fish fed a commercial feed (Com), commercial diet + protease complex (PC; AG175 by Jefo Nutrition; 175 g 1000 kg⁻¹), and commercial feed + humic substance (HS; MFG50 by Kent Nutrition; 2500 g 1000 kg⁻¹). The feeding trial was conducted for 60 days. A deep-water culture hydroponic system with nine 109 L troughs was used. Each trough corresponded to each tank and contained 15 romaine lettuce plants. The first 4 weeks were fish growth trials, and the last four were fish and plant growth trials. Fish and plant growth parameters showed no differences between treatments. However, the leaf greenness in romaine lettuce plants in the PC treatment had significantly higher chlorophyll content than the other treatments (<i>p</i> = 0.002). When microbial communities were analyzed, significant differences were found in the Shannon diversity indices (fecal, water, and root <i>p</i> &lt; 0.001), Chao1 (fecal <i>p</i> &lt; 0.002, water <i>p</i> &lt; 0.001, and root <i>p</i> &lt; 0.001), and observed species (feca; <i>p</i> = 0.046, water <i>p</i> = 0.017, and root <i>p</i> = 0.015). When beta diversity was analyzed through the Bray–Curtis dissimilarity index, fecal samples (<i>p</i> = 0.001) and root samples (<i>p</i> = 0.002) showed clear differences. The most abundant phyla found in all samples were Pseudomonadota. Overall, this study concluded that these additives did not affect the growth of Nile tilapia and romaine lettuce but influenced the bacterial composition of fish feces, water, and root samples and the chlorophyll content of the plants.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4062437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mortality Risk of Juvenile Japanese Sea Cucumber Apostichopus japonicus by the Sympatrically Occurring Hermit Crab Paguristes ortmanni","authors":"Nobuharu Inaba,&nbsp;Takuma Matsumoto,&nbsp;Yuji Anaguchi,&nbsp;Kohei Matsuno","doi":"10.1155/are/5245463","DOIUrl":"https://doi.org/10.1155/are/5245463","url":null,"abstract":"<div>\u0000 <p>The density of the hermit crab, <i>Paguristes ortmanni</i>, in the artificial reef of juvenile <i>Apostichopus japonicus</i> increased almost threefold from June to December 2018. Calcareous ossicles of <i>A. japonicus</i> were identified from the stomach contents in 28.8% of the hermit crabs (18.6% in males and 10.2% in females) in December; season hatchery-produced juveniles are frequently introduced into the wild in Hokkaido, Japan. The mortality rate of juvenile <i>A. japonicus</i> by <i>P. ortmanni</i> was estimated to be 2.5 ± 2.4 individuals day⁻¹ based on laboratory predation experiments. Interestingly, 3% to 5% of individuals survived despite being attacked and injured in all trials, escaping on the shells of hermit crabs. Over 50% of females in the ossicle-not-detected group had shield lengths (SLs) smaller than the smallest individual in the ossicle-detected group. The average SL of the ossicle-detected group in females was significantly higher (<i>p</i>&lt; 0.01) than that of the not-detected group, indicating an increased predation risk for <i>A. japonicus</i> juveniles when larger female <i>P. ortmanni</i> were present. The present study offers new insights into the predatory behavior of <i>P. ortmanni</i> toward <i>A. japonicus</i> juveniles, showing that these sympatric hermit crabs present a considerably high mortality risk to <i>A. japonicus</i> juveniles. It also emphasizes the importance of implementing appropriate measures to protect juveniles from predators during the release process, providing an essential viewpoint for enhancing and rebuilding the wild population of commercially important endangered <i>A. japonicus</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5245463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling Gastric Evacuation Rates in Fish With a General Power Function: A Step-by-Step Guide to Parameter Estimation and Analysis Using R Statistical Software","authors":"Ömerhan Dürrani","doi":"10.1155/are/7309111","DOIUrl":"https://doi.org/10.1155/are/7309111","url":null,"abstract":"<div>\u0000 <p>Accurate modelling of gastric evacuation (GE) is a prerequisite for optimising feeding strategies in aquaculture and improving predator–prey interaction models in ecological studies. However, GE analysis is challenging because of the interdependencies of the variables influencing the evacuation process. To overcome this, a general power function was developed in 1992 and refined in 1998 using non-linear regression techniques. The proposed method is robust for identifying the best-fit evacuation function while simultaneously assessing the effects of potential predictor variables. This study demonstrated the application of R statistical software for parameter estimation of the general power function to characterise the influence of fish size, meal size and temperature on GE rate (GER) in brown trout (<i>Salmo trutta</i> Linnaeus, 1758) and rainbow trout (<i>Oncorhynchus mykiss</i> Walbaum, 1792). The statistical methods presented are remarkably adaptable and applicable to a wide range of GE datasets. Furthermore, they can be extended to incorporate additional variables influencing GE, making them integral tools for advancing research in fisheries management, ecological modelling and aquaculture.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7309111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Seawater Temperature on the Performance of the Tropical Sea Urchin Lytechinus variegatus (Lamarck, 1816)","authors":"A. M. Lemus-Granados,&nbsp;M. A. Olivo-Cantillo,&nbsp;K. Medina-Lambraño,&nbsp;A. Rodríguez-Forero","doi":"10.1155/are/4003916","DOIUrl":"https://doi.org/10.1155/are/4003916","url":null,"abstract":"<div>\u0000 <p>As seawater temperatures rise in the ocean due to global temperature change, altered mean precipitation, and weather patterns, native species face uncertain futures. The alteration in thermal tolerance was assessed in wild-captured tropical sea urchins (<i>Lytechinus variegatus</i>) from the Caribbean Sea and then maintained under controlled conditions. Three experiments were performed: (1) favorable growth temperature: after maintaining wild sea urchins at three different seawater temperatures (22, 24 and 26°C) for 70 days, it was observed that 22°C was the best temperature for growth performance. Sea urchins showed daytime behavior (higher activity at 9:00−10:00 h), during this time period; (2) thermal preference (TP): determined with the help of a horizontal camera with a thermal gradient (19–30°C), it showed that the acclimation temperature influenced the TP of sea urchins, since a significantly higher number of animals opted for the temperature at which they had been acclimated; and (3) critical thermal maximum (CTmax) and critical thermal minimum (CTmin): sea urchins exposed to a progressive temperature change, starting from the acclimation temperature, tolerated the increase in temperature (up to 14°C) better than the decrease (up to 12°C). These results thus contribute to our knowledge of sea urchin thermal biology and provide information about the assessment of thermal comfort conditions in sea urchin farming.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4003916","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Bacillus Probiotics: An Approach for Vibrio Pathogen Control and Organic Sludge Management in Shrimp Aquaculture","authors":"Harikumar Sampath,&nbsp;Guruchandran Veerasamy,&nbsp;Rajalekshmi Mukkalil","doi":"10.1155/are/2767593","DOIUrl":"https://doi.org/10.1155/are/2767593","url":null,"abstract":"<div>\u0000 <p>Probiotics are essential in shrimp aquaculture as they help improve water and soil quality, ultimately enhancing productivity. This study assesses the probiotic potential of six <i>Bacillus</i> strains by evaluating various functional traits. The inhibitory activity of culture supernatants from each strain was tested against two <i>Vibrio parahaemolyticus</i> strains and <i>V. vulnificus</i> using a growth kinetics assay. Additionally, the ability of these strains to degrade soil organic matter was evaluated by observing their growth in liquid media containing shrimp pond sludge. The strains were characterized by 16S rRNA sequencing and evaluated for carbohydrate fermentation profiles using an analytical profile index (API) system. Safety assessments included hemolysis, antibiotic sensitivity, and a toxicity assay using <i>Artemia salina</i>. Salinity tolerance was assessed through growth kinetics at varying salt concentrations. <i>Bacillus</i> strains PS1, PS2, and PS5 exhibited significant inhibition against <i>V. parahaemolyticus</i>, while PS1, PS6, and PS5 effectively inhibited <i>V. vulnificus</i>. Strains PS1, PS2, PS5, and PS6 showed strong potential for sludge degradation. Identified as <i>Bacillus pumilus</i> PS1, <i>B. licheniformis</i> PS2, <i>B. subtilis</i> PS5, and <i>B. subtilis</i> PS6, these strains were non-hemolytic and sensitive to common antibiotics, including ampicillin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. High survival rates of <i>A. salina</i> in the presence of these <i>Bacillus</i> strains confirmed their safety for shrimp use. Additionally, these strains demonstrated growth across a wide salinity range (5–55 practical salinity units). This study supports the suitability and safety of <i>B. pumilus</i> PS1, <i>B. licheniformis</i> PS2, <i>B. subtilis</i> PS5, and <i>B. subtilis PS6</i> for shrimp aquaculture applications.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/2767593","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}