Aquaculture Research最新文献

{"title":"A Literature Review of Tilapia/Lettuce Aquaponics—Production Status, Varieties, and Research Gaps","authors":"Aya S. Hussain,&nbsp;Paul B. Brown","doi":"10.1155/2024/2642434","DOIUrl":"https://doi.org/10.1155/2024/2642434","url":null,"abstract":"<div>\u0000 <p>Tilapia and lettuce are the most frequently used combination among aquaponics producers, hobbyists, and educators. Therefore, this literature review aims to aggregate the knowledge on the current status of tilapia/lettuce production and identify research gaps. Among the 40 reviewed publications, 72.5% used Nile tilapia, 17.5% used red, 2.5% used red Mozambique, 5% used rocky mountain strain, and 5% of the articles stated that they used tilapia without reporting the species. Tilapia initial density used ranged from 0.34 to 28.4 kg/m³, which depended on the purpose of the study, experimental size, and design. For the hydroponic unit, 55% of the publications utilized deep water culture (DWC), 35% utilized nutrient film technique (NFT), and 10% utilized media bed (MB). The optimum ratio between fish feeding rate and plant growing area (60 g of feed/day/m²) was originally calculated at the University of Virgin Island and published in 1988 using Bibb lettuce. This recommended ratio has been used by farmers and researchers as a rule of thumb for years. However, this recommended ratio did not take into account other factors such as fish species, protein content of the diet, plant species/variety, plant density, biological filtration, and airflow in the grow bed. Hence, the current review suggests that the fish-to-plant ratio, fish density, and flow rate in the aquaponic systems needs to be reevaluated. As there is a thermal preference mismatch between tilapia and lettuce, there is a need to evaluate the optimum temperature for both to obtain the highest growth performance. Nowadays, it is believed that the farmed stocks’ genetic quality needs to be regularly enhanced and protected. Consequently, feeding frequency, feeding amount, and stocking densities in aquaponic systems should be continuously reevaluated with the improved strains of tilapia as well as heat-tolerant varieties of lettuce.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/2642434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Starvation on the Serum Physiology, Transcriptome and Intestinal Microbiota of Juvenile Largemouth Bass (Micropterus salmoides)","authors":"Huan Wang,&nbsp;Huaxing Zhou,&nbsp;He Jiang,&nbsp;Yuting Hu,&nbsp;Tong Li,&nbsp;Guoqing Duan","doi":"10.1155/2024/8961174","DOIUrl":"https://doi.org/10.1155/2024/8961174","url":null,"abstract":"<div>\u0000 <p>Starvation is common in fish, particularly during the juvenile period, and is typically caused by environmental changes. However, the molecular mechanisms of juvenile <i>Micropterus salmoides</i> in response to starvation are still poorly understood. We elucidated changes in the serum physiology, transcriptome, and intestinal microbiome of <i>M. salmoides</i> after 14 days of starvation. Body weight and serum concentrations of glucose, cholesterol, triglycerides, and alkaline phosphatase significantly decreased during the starvation period. However, serum concentrations of aspartate transaminase and alanine transaminase were significantly elevated. Transcriptome analysis of liver tissues from commonly fed fish and starved fish revealed 1069 differentially expressed genes (DEGs), of which 211 were up-regulated. In total, 586 DEGs were identified in the intestinal tissues, of which 166 were up-regulated and 420 were down-regulated. KEGG analysis revealed that starvation affected multiple pathways, particularly those related to energy, metabolism and immunity. Starvation limited fatty acid biosynthesis and elongation in the liver, down-regulated immunity-related genes in the intestine and promoted fatty acid degradation. The 16S rDNA analysis revealed that starvation altered intestinal microbiota homeostasis and increased intestinal Shannon diversity. Two opposite patterns of dominant genera emerged in commonly fed fish (most abundant genus is <i>Mycoplasma</i>) and in starved fish (most abundant genus is <i>Cetobacterium</i>). Starvation also increased the abundance of pathogens, including <i>Plesiomonas</i> and <i>Aeromonas</i>. Our study will provide the theoretical basis for assessing the starvation levels and nutritional status of juvenile fish and guidance for further studies on starvation in the context of fish physiology and health.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8961174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142174291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Mysis diluviana as a Substitute for Fishmeal in Formulated Aquafeed for Fingerling Rainbow Trout","authors":"Louis A. Gosselin,&nbsp;Brian A. Heise,&nbsp;Ravinder Sappal,&nbsp;Austin N. Pietramala,&nbsp;Kingsley K. Donkor","doi":"10.1155/2024/3774463","DOIUrl":"https://doi.org/10.1155/2024/3774463","url":null,"abstract":"<div>\u0000 <p>The present study explored the potential of wild <i>Mysis diluviana</i>, a widespread freshwater crustacean that is also invasive in many lakes, to serve as a substitute for fishmeal in the formulation of salmonid aquafeed. <i>M. diluviana</i>, harvested from Okanagan Lake in British Columbia, Canada, had a high protein content, with crude protein making up 53% of its dry body weight; lipids formed 32% of dry <i>M. diluviana</i> body weight. Fingerlings from two distinct strains of rainbow trout were fed for 60 days with one of three diet treatments: experimental feeds in which 25% or 50% of the fishmeal ingredient was replaced with <i>M. diluviana</i> and a feed containing no <i>M. diluviana</i>. Feeds containing <i>M. diluviana</i> supported the same survivorship and similar or better fingerling growth as the fishmeal-only feed, including fingerlings of a trout strain that are highly selective feeders. The amino acid and fatty acid composition of fingerling tissues at the end of the experiment was mostly indistinguishable among the three feed treatments. In addition, the tissue composition of fingerlings, such as the EAA : NEAA ratio and the amounts of SFA, MUFA, and PUFA, did not fully match the biochemical composition of their feeds, likely due in part to the capacity of freshwater salmonids for bioconversion of some amino acids into other less available amino acids. <i>M. diluviana</i> was therefore found to be an effective source of protein and lipid for fingerling rainbow trout and an effective substitute for marine fishmeal in trout aquafeed.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3774463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Dietary Etlingera elatior (Jack) Bud Flower Powder against Edwardsiella tarda Infection in African Catfish, Clarias gariepinus","authors":"Seong Wei Lee,&nbsp;Vui Kien Liew,&nbsp;Zulhisyam Abdul Kari,&nbsp;Muhammad Anamul Kabir,&nbsp;M. N. Azra,&nbsp;Martina Irwan Khoo,&nbsp;Wendy Wee","doi":"10.1155/2024/1754005","DOIUrl":"https://doi.org/10.1155/2024/1754005","url":null,"abstract":"<div>\u0000 <p>This study investigates the impacts of dietary <i>Etlingera elatior</i> (Jack) bud flower powder (EE) supplementation on the growth and health status of African catfish, <i>Clarias gariepinus</i>. Each treatment group received different formulated diets: basal diet without EE (control), basal diet + 1% EE (EE1), basal diet + 2% EE (EE2), basal diet + 3% EE (EE3), and basal diet + 4% EE (EE4). After an 8-week feeding trial, fish supplemented with dietary EE exhibited significantly improved growth performance, such as weight gain (WG; 1,251.4%–1,604.3%; <i>p</i> &lt; 0.0001), specific growth rate (SGR; 2.02%–2.20%; <i>p</i> &lt; 0.0001), and final weight (FW; 141.7–178.9 g; <i>p</i> &lt; 0.0001) than the control group (WG: 1,192.0%, SGR: 1.98%, and FW: 136.1 g), particularly EE2 and EE3. Furthermore, EE-treated fish recorded significantly lower feed conversion rate (FCR; 1.19–1.53; <i>p</i> &lt; 0.0001), viscerosomatic index (VSI; 3.40%–4.50%; <i>p</i> &lt; 0.0001), and hepatosomatic index (HSI; 3.13%–4.40%; <i>p</i> &lt; 0.0001) than the control (FCR: 1.59, VSI: 5.48%, and HSI: 4.75%), particularly EE2 and EE3. The EE-treated fish also had significantly higher white blood cell (WBC) count (124.6–148.6/<i>µ</i>L; <i>p</i> &lt; 0.0400), red blood cell (RBC) count (2.43–4.03 × 10³/<i>µ</i>L; <i>p</i> &lt; 0.0002), hemoglobin (HGB) concentration (6.27–7.87 g/dL; <i>p</i> &lt; 0.0160), and hematocrit (HCT; 26.8%–38.7%; <i>p</i> &lt; 0.0200) compared to the control (WBC count: 112.1/<i>µ</i>L, RBC count: 2.17 ×10³/<i>µ</i>L, HGB concentration: 5.60 g/dL, and HCT: 23.7%), with the highest being EE2 and EE3. Dietary EE diets enhanced digestive enzyme activities, including amylase (<i>p</i> &lt; 0.0090), protease (<i>p</i> &lt; 0.0040), and lipase (<i>p</i> &lt; 0.0060), significantly (<i>p</i> &lt; 0.05) than the control, where EE2 and EE3 demonstrated the highest activities. The EE supplementation also significantly improved the fish’s antioxidative responses, particularly catalase (CAT; <i>p</i> &lt; 0.0100), glutathione peroxidase (GPx; <i>p</i> &lt; 0.0300), and superoxide dismutase (SOD; <i>p</i> &lt; 0.0100) in EE2 and EE3. Similarly, the cumulative survival rate of EE2 (66.7% ± 5.77%) and EE3 (66.7% ± 5.77%) were significantly (<i>p</i> &lt; 0.0001) higher than other groups post-<i>Edwardsiella tarda</i> challenge. Therefore, this study findings highlighted the potential benefits of EE as a feed additive to boost the production in African catfish farming.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/1754005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity and Population Structure of Eight Cultured Hypophthalmichthys molitrix in Hubei Province, China, Inferred from Mitochondrial COI Gene","authors":"Heng Ge,&nbsp;Honghui Guo,&nbsp;Yajun Wang,&nbsp;Hang Sha,&nbsp;Guiwei Zou,&nbsp;Yi Chai,&nbsp;Hongwei Liang","doi":"10.1155/2024/8492751","DOIUrl":"https://doi.org/10.1155/2024/8492751","url":null,"abstract":"<div>\u0000 <p>Silver carp’s (<i>Hypophthalmichthys molitrix</i>) traits and genetic structure are being impacted by artificial proliferation and restocking enhancement. A clear genetic background of cultured silver carp is helpful for exploitation and utilization. Limited research reported the germplasm resources of cultured silver carp. This study was conducted to investigate the genetic diversity of cultured silver carp. Two hundred thirty-three silver carps were sampled from eight cultured populations in Hubei province, and their population structures were analyzed by mitochondrial COI gene. Average contents of bases T, C, A, and G in the 659 bp COI gene sequence were 30.03%, 26.62%, 26.08% and 17.32%, respectively. And 18 haplotypes were defined from 77 variable nucleotides in COI gene. The haplotypes and nucleotide diversities were 0.604 and 0.00325, respectively. Meanwhile, the highest genetic diversity and lowest genetic diversity were detected in cultured populations from Jianli population (Hd: 0.883 and <i>π</i>: 0.00699) and Yaowan population (Hd: 0.186 and <i>π</i>: 0.00085), respectively. Pairwise fixation index (<i>Fst</i>) analysis revealed that the level of genetic diversity was moderate (<i>Fst</i>: 0.06). The genetic distance between and within populations were 0.00353 and 0.00329, respectively. And the genetic variation occurred mainly within populations (93.42%), but genetic variation between the population was only 6.58%. Therefore, moderate-level genetic diversity was observed with high haplotype diversity and low nucleotide diversity, suggesting that inbreeding should be avoided among the eight cultured populations in Hubei.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8492751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Starvation and Refeeding on the Compensatory Growth of Juvenile Epinephelus coioides","authors":"Yukai Yang,&nbsp;Wei Yu,&nbsp;Yafei Duan,&nbsp;Heizhao Lin,&nbsp;Zhong Huang,&nbsp;Xiaolin Huang,&nbsp;Tao Li","doi":"10.1155/2024/9986750","DOIUrl":"https://doi.org/10.1155/2024/9986750","url":null,"abstract":"<div>\u0000 <p>The purpose of this study was to investigate the adaptive strategies and compensatory growth mechanisms of juvenile <i>Epinephelus coioides</i> under starvation stress. The fish with an initial body weight of 8.88 ± 0.06 g were starved for 0 day (F0, control), 3 days (F3), 6 days (F6), 9 days (F9), 12 days (F12), and 15 days (F15), followed by feeding periods of 30 days, 27 days, 24 days, 21 days, 18 days, and 15 days, respectively. The results are shown as follows: (1) <i>E. coioides</i> could achieve complete or partial compensatory growth by improving both feeding rate and feed conversion efficiency. (2) The viscerosomatic index and the hepatosomatic index of the test fish decreased linearly with the duration of starvation (<i>P</i> &lt; 0.05), while the condition factor did not significantly decrease (<i>P</i> &gt; 0.05). At the end of feeding, all the physical body indicators of the test fish recovered or exceeded the control levels. (3) The lipid and glycogen contents of the test fish decreased continuously throughout the entire starvation process, with noticeable protein consumption occurring in the later stages of starvation (<i>P</i> &lt; 0.05). At the end of the feeding period, apart from a significant reduction in crude lipid contents in the F12 and F15 groups compared to the control group (<i>P</i> &lt; 0.05), the biochemical composition of the test fish was restored or even surpassed that of the control group. (4) The activity of digestive enzymes could be stimulated by starvation, enabling the efficient utilization of energy storage substances. In conclusion, it is advisable to limit the period of starvation for juvenile <i>E. coioides</i> to 12 days, which means that the ratio of starvation period to feeding period should not exceed two-thirds.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/9986750","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of Domesticated Tiger Shrimp Postlarvae (Penaeus monodon) and Mud Crab (Scylla paramamosain) in Extensive Rice-Shrimp Ponds","authors":"Tung Hoang,&nbsp;Binh Thai Nguyen,&nbsp;Quang Viet Huynh","doi":"10.1155/2024/5773446","DOIUrl":"https://doi.org/10.1155/2024/5773446","url":null,"abstract":"<div>\u0000 <p>This study assessed the performance of domesticated tiger shrimp <i>Penaeus monodon postlarvae</i> and mud crab <i>Scylla paramamosain</i> co-cultured in 90 extensive rice-shrimp ponds in the Mekong River Delta of Vietnam from 2022 to Sep 2023. Limited supplementary feeding was applied for only 20% of the studied ponds. Results show that ponds stocked with domesticated postlarvae produced significantly larger shrimps at harvest and had better postlarvae usage efficiency (<i>P</i> &lt; 0.05) compared with those stocked with older postlarvae produced by wild-caught broodstock. Furthermore, a combination of stocking domesticated postlarvae and using organic fertilizer for pond preparation and during the crop was found to significantly improve productivity by 28% and final harvest size by 67% (<i>P</i> &lt; 0.05). Larger shrimps fetched 54% higher farm-gate prices, thus significantly increasing gross revenue for farmers. Co-culture of mud crab in rice-shrimp ponds at low densities (0.2–0.7 pcs/m²) was popular and profitable. Productivity was 116–187 kg/ha/crop with a profit margin up to 92.8%. Income from mud crab was very similar to that from shrimp. The results of this study suggest that domesticated postlarvae of <i>P. monodon</i> should be used for stocking extensive systems, preferably in combination of using organic fertilizer to enhance natural foods. Further improvement of income and profit can be achieved if more attention is paid on crablet quality and quality improvement.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5773446","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Healthy Liver of Largemouth Bass (Micropterus salmoides) in the Short Term by Steroidal Saponins before Heat Season Comes","authors":"Tao Cheng,&nbsp;Jiandong Chen,&nbsp;Xiangdong Shen,&nbsp;Guiqiong Chen,&nbsp;Beiping Tan,&nbsp;Shuyan Chi","doi":"10.1155/2024/3496627","DOIUrl":"https://doi.org/10.1155/2024/3496627","url":null,"abstract":"<div>\u0000 <p>Spotlighting the current state of largemouth bass (<i>Micropterus salmoides</i>) aquaculture, this study exhibited the effects of steroidal saponins (SS) on the liver health of largemouth bass under high-temperature stress. The study consisted of two experiments: the first trial was designed to evaluate the growth performance of fish fed the experimental diets containing SS 0% and 0.1% (SS₀ and SS_0.1) for 30 days. And then, the second trial was used to evaluate the health of the livers of these fish under high-temperature (34°C) stress which lasts for 1 week, and the groups were named as SS₀, HSS₀, SS_0.1, and HSS_0.1, respectively. In the first feeding trial, except for the higher feeding rate observed in the SS_0.1 group (<i>P</i> &lt; 0.05), no significant difference was observed between the groups SS₀ and SS_0.1 in survival rate, weight gain rate, specific growth rate, and feed conversion rate, respectively. After 1 week of high-temperature stress, the specific growth rate and weight gain rate of fish were significantly lowered in the HSS₀ and HSS_0.1 groups (<i>P</i> &lt; 0.05). In terms of feeding rate, the SS_0.1 and HSS_0.1 groups were significantly higher than the other two groups (<i>P</i> &lt; 0.05). Under high-temperature stress, gene expressions of <i>caspase-3</i>, <i>caspase-8</i>, <i>caspase-9</i>, <i>tnf-α</i>, <i>il-8</i>, and <i>hsp70</i> were significantly downregulated in the liver of largemouth bass in the HSS_0.1 group (<i>P</i> &lt; 0.05). The activities of antioxidant enzymes, including catalase, total superoxide dismutase, and glutathione peroxidase in the liver, were significantly increased, while the serum enzyme activities of aspartate aminotransferase and alanine aminotransferase were significantly decreased in the HSS_0.1 group (<i>P</i> &lt; 0.05). Compared to the HSS₀ group, hepatic vacuolization areas of fish in the HSS_0.1 group were improved (<i>P</i> &lt; 0.05). Therefore, the results of the study supported that the 0.1% SS used in largemouth bass diet for 30 days could strengthen the liver and body health and help fish resist heat stress.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3496627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Cryorepository for Coral Larvae: Safeguarding Corals for Future Generations","authors":"Kanokpron Loeslakwiboon,&nbsp;Wen-Chung Hsieh,&nbsp;Cheng-Liang Huang,&nbsp;Sujune Tsai,&nbsp;Chiahsin Lin","doi":"10.1155/2024/4887191","DOIUrl":"https://doi.org/10.1155/2024/4887191","url":null,"abstract":"<div>\u0000 <p>Research on the development of innovative cryobanking techniques can aid coral restoration and conservation. This study applied a customized freezing device and “cryojig” along with vitrification and laser warming techniques to create the first cryorepository for pelagic-phase larvae of the corals <i>Seriatopora caliendrum</i>, <i>Pocillopora verrucosa</i>, <i>Stylophora pistillata</i>, and <i>Pocillopora acuta</i>. Three vitrification solutions (VSs) were prepared, with each composed of two of four cryoprotective agents. These included VS1: 2 M ethylene glycol (EG) and 1 M propylene glycol; VS2: 2 M EG and 1 M glycerol; VS3: 2 M dimethyl sulfoxide and 1 M EG. Larvae were then mixed with the VSs, 40% (w/v) Ficoll, and 10% (v/v) gold nanoparticles (GNPs) at a final concentration of 1.2 × 10¹⁸ particles/m³ and using our customized device for vitrification and laser warming. Over a thousand coral larvae (576 <i>S. caliendrum</i>, 376 <i>P. verrucosa</i>, 238 <i>S. pistillata</i>, and 524 <i>P. acuta</i>) were successfully stored in the cryorepository and the proportions of coral larvae that were noted to be actively swimming or settled after vitrification and laser warming. This is the first project to establish a coral larvae cryorepository, and the methods applied could represent a critical tool for reef restoration and maintenance of marine biodiversity.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4887191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between Water Quality Parameters and Parasite Infestation in Farmed Oreochromis niloticus in Selected Rift Valley Counties, Kenya","authors":"Finnan O. Ageng’o,&nbsp;Robert M. Waruiru,&nbsp;Daniel W. Wanja,&nbsp;Philip N. Nyaga,&nbsp;Mercy M. Hamisi,&nbsp;Cidee N. Khasake,&nbsp;Jacob M. Wainaina,&nbsp;Beatrice M. Munde,&nbsp;Paul G. Mbuthia,&nbsp;Nichodemus M. Kamuti,&nbsp;Shimaa E. Ali,&nbsp;Saraya Tavornpanich,&nbsp;Edith A. Keya,&nbsp;Mohan V. Chadag","doi":"10.1155/2024/6139798","DOIUrl":"https://doi.org/10.1155/2024/6139798","url":null,"abstract":"<div>\u0000 <p>Globally, parasitic infections hinder aquaculture productivity and sustainability. Improper water quality parameters reduce fish immunity, make them susceptible to parasites, and enhance parasitic proliferation in culture systems. Subsequently, this study aimed to determine the prevalence and intensity of ecto- and endo-parasite infesting <i>Oreochromis niloticus</i> in varying water quality in aquaculture systems in Kericho, Bomet, and Nakuru counties. A total of 220 <i>Oreochromis niloticus</i> samples were captured from 11 liner ponds, eight earthen ponds, two concrete ponds, and one plastic tank from 22 farms in the three counties. External and internal organs were examined for parasite infestation. Skin scrapings and squash preparation of fish eyes were also examined to reveal the organisms through microscopic examination. A Pearson’s correlation analysis between physicochemical parameters and parasitic prevalence was used to determine the strength and direction of correlation coefficients with significance at 95%. Overall parasitic prevalence infestation was 39.6% (87/220). Ten parasite genera were recovered, with <i>Dactylogyrus</i> species dominating overall at 15.9% (35/220), followed by <i>Trichodina</i> spp. (7.7%; 17/220). <i>Epistylis</i> species had the highest mean intensity of 12.8 ± 35.7 (total number of <i>Epistylis</i> parasites (141)/total of infested fish (11)). Earthen ponds had a significantly higher (36.2%, 29/80) prevalence of <i>Dactylogyrus</i> sp. than liner ponds (3.6%, 4/110) (<i>p</i> &lt; 0.001). Dissolved oxygen levels were 16.9 ± 0.7 mgL⁻¹ in Bomet, 14.9 ± 0.3 mgL⁻¹ in Kericho, and 12.5 ± 0.5 mgL⁻¹ in Nakuru which were above desirable range for fish culture. The electrical conductivity in Bomet was 76 ± 5 <i>µ</i>S/cm and Kericho 52 ± 4 <i>µ</i>S/cm below the desirable range. Temperature, pH, phosphate, nitrates, and nitrites were within the desirable limit for fish farming. Electrical conductivity and pH had positive correlation of 1 and 0.97, respectively, with the prevalence of <i>Acanthocephalus</i>, <i>Euclinostomum</i>, and <i>Trichodina</i> species. Temperature and nitrate positively correlated between 0.80–0.89 and 0.90–0.99, respectively, with the prevalence of <i>Dactylogyrus</i>, <i>Riboscyphidia</i>, <i>Epistylis</i>, <i>Camallanus</i>, and <i>Paracamallanus</i> species. The study demonstrates that water quality significantly influences parasitic prevalence in aquaculture systems. Therefore, it is crucial for farmers to monitor water quality parameters to mitigate parasitic infestations.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6139798","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142077812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}