Hao-ran Yang, Herbert Brightmore Munyaradzia, Wen-bin Zhu, Zai-jie Dong, Lan-mei Wang
{"title":"Effects of Cold Stress on Skin Color, Immuno-Antioxidant Function, Muscle Compositions, and Intestinal Microbiota of Red Tilapia During Overwintering","authors":"Hao-ran Yang, Herbert Brightmore Munyaradzia, Wen-bin Zhu, Zai-jie Dong, Lan-mei Wang","doi":"10.1155/are/9910096","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Cold stress due to overwintering is considered a major issue limiting the development of the red tilapia (<i>Oreochromis</i> spp.) industry. This study was the first to systematically investigate the effects of cold stress on skin color, immuno-antioxidant function, muscle compositions, and intestinal microbiota of red tilapia during overwintering. Fish (initial weight: 72.71 ± 1.32 g) were divided into the cold stress group (cold) and the control group. In the control group, the water temperature (WT) was maintained at 20°C, which is consistent to with the overwintering WT indoors or greenhouse in the local area. In the cold group, the WT was decreased from 20°C to 8°C by 2°C per day during the experiment. At the end of the experiment, we found that the <i>L</i><sup>∗</sup> value of fish ventral skin in the cold group was lower than that in the control group (<i>p</i> < 0.05). The superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), and total antioxidant capacity (T-AOC) activities were higher, but complement 3 (C3) and complement 4 (C4) activities were lower in the liver of cold group fish than those in the control group (<i>p</i> < 0.05). For the gill tissue, the SOD, MDA, and GSH activities were higher, but the C3 activity was lower in the cold group than those in the control group (<i>p</i> < 0.05). The total amino acid (TAA) content in the muscle of fish in the cold group was higher than that in the control group (<i>p</i> < 0.05). 16 s rRNA analysis showed that the intestinal microbial abundance and alpha diversity of fish in the cold group decreased (<i>p</i> < 0.05). Component analysis revealed a significantly higher relative abundance of <i>Cetobacterium</i> spp. in the fish of the cold group. The findings of this study will provide a valuable understanding of fish cold stress during overwintering, which will contribute to facilitating the breeding of new cold-resistant varieties of red tilapia in the future.</p>\n </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9910096","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Research","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/are/9910096","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
Cold stress due to overwintering is considered a major issue limiting the development of the red tilapia (Oreochromis spp.) industry. This study was the first to systematically investigate the effects of cold stress on skin color, immuno-antioxidant function, muscle compositions, and intestinal microbiota of red tilapia during overwintering. Fish (initial weight: 72.71 ± 1.32 g) were divided into the cold stress group (cold) and the control group. In the control group, the water temperature (WT) was maintained at 20°C, which is consistent to with the overwintering WT indoors or greenhouse in the local area. In the cold group, the WT was decreased from 20°C to 8°C by 2°C per day during the experiment. At the end of the experiment, we found that the L∗ value of fish ventral skin in the cold group was lower than that in the control group (p < 0.05). The superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), and total antioxidant capacity (T-AOC) activities were higher, but complement 3 (C3) and complement 4 (C4) activities were lower in the liver of cold group fish than those in the control group (p < 0.05). For the gill tissue, the SOD, MDA, and GSH activities were higher, but the C3 activity was lower in the cold group than those in the control group (p < 0.05). The total amino acid (TAA) content in the muscle of fish in the cold group was higher than that in the control group (p < 0.05). 16 s rRNA analysis showed that the intestinal microbial abundance and alpha diversity of fish in the cold group decreased (p < 0.05). Component analysis revealed a significantly higher relative abundance of Cetobacterium spp. in the fish of the cold group. The findings of this study will provide a valuable understanding of fish cold stress during overwintering, which will contribute to facilitating the breeding of new cold-resistant varieties of red tilapia in the future.
期刊介绍:
International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.