Aquaculture Research最新文献

{"title":"Brain Transcriptome Profiles of Greater Amberjack (Seriola dumerili) Juveniles Under Long-Term Hypo- and Hypersaline Stress","authors":"Xiaoying Ru,&nbsp;Yang Huang,&nbsp;Tong Zhou,&nbsp;Tonglin Yang,&nbsp;Peipei Chen,&nbsp;Jiahui Yang,&nbsp;Chunhua Zhu,&nbsp;Hongjuan Shi","doi":"10.1155/are/2157396","DOIUrl":"https://doi.org/10.1155/are/2157396","url":null,"abstract":"<div>\u0000 <p>The fish brain plays a crucial role in regulating growth, reproduction, development, and adaptation to environmental stress. However, there are few studies that have examined the entire fish brain transcriptome and its responses to long-term hypo- and hyper-salinity stress. Greater amberjack (<i>Seriola dumerili</i>) has a high commercial value in mariculture worldwide due to its high growth rate and excellent flesh quality. Consequently, high-throughput RNA-Seq was employed to elucidate the molecular regulatory mechanisms underlying salinity adaptation by identifying gene expression changes in the brain of greater amberjack exposed to elevated and/or reduced salinity environments. We subjected individuals to salinity levels of 20, 30, and 40 ppt (parts per thousand) for 30 days. A total of 272 (198 up-regulated and 74 down-regulated) differentially expressed genes (DEGs) were identified in the B30 vs. B20 group, 21 (10 up-regulated and 11 down-regulated) DEGs in the B30 vs. B40 group, and 343 (119 up-regulated and 224 down-regulated) DEGs in the B20 vs. B40 group. Transcriptomic analysis revealed that salinity stress influenced the expression of genes associated with amino acid metabolism and transport (<i>gpt</i>, <i>arg2</i>, <i>LOC111237759</i>, <i>slc3a2</i>, and <i>slc7a5</i>), carbohydrate metabolism (<i>aldob</i>, <i>ldhba</i>, and <i>gapdh</i>), and signal transduction (<i>map3k8</i>, <i>map3k2</i>, <i>map2k7</i>, and <i>lepr</i>). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that the DEGs were significantly enriched in metabolism pathways, especially in amino acid and carbohydrate metabolism, transcription, translation, et cetera. Furthermore, gene set enrichment analysis (GSEA) demonstrated that the metabolism, signal transduction, translation, immune system, and transport and catabolism pathways were more active in the brain. These findings provide a foundation for further studies to clarify the molecular mechanisms of salinity adaptation and transcriptional regulation in the brain of marine fish.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/2157396","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143113618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Salinity on Nursery Bi-Culture of Pacific White Leg Shrimp (Litopenaeus vannamei) and Giant Prawn (Macrobrachium rosenbergii) in a Biofloc System","authors":"Md. Abdul Halim,&nbsp;Dania Aziz,&nbsp;Aziz Arshad,&nbsp;Nur Leena W. S. Wong,&nbsp;Murni Karim,&nbsp;Md. Ariful Islam,&nbsp;Fadhil Syukri,&nbsp;Md. Lifat Rahi","doi":"10.1155/are/5368636","DOIUrl":"https://doi.org/10.1155/are/5368636","url":null,"abstract":"<div>\u0000 <p>The current study was carried out to examine the effects of four different experimental salinities (T₁ = 0‰, T₂ = 5‰, T₃ = 10‰, and T₄ = 15‰) on growth, water quality, proximate composition, total bacterial (TB), and hemocyte counts of white leg shrimp (<i>Litopenaeus vannamei</i>) and giant prawn (<i>Macrobrachium rosenbergii</i>) in biofloc based nursery bi-culture system for 6 weeks. A total of 12 cylindrical plastic tanks (125 L) filled up 100 L water for rearing <i>L. vannamei</i> and <i>M. rosenbergii</i> post-larvae (PLs) at an equal ratio: (50 <i>L. vannamei</i>: 50 <i>M. rosenbergii</i>). At the end of the experiment, for <i>L. vannamei</i>, the significantly higher (<i>p</i> &lt; 0.05) growth rate was recorded in T₄ (15‰) compared to the other treatments. For <i>M. rosenbergii</i>, a significantly higher (<i>p</i> &lt; 0.05) growth rate was recorded in T₂ (5‰) than in other treatments. Similar to growth, the best (<i>p</i> &lt; 0.05) feed conversion ratio (FCR) for -<i>L. vannamei</i> was found at T₄ (15‰) while it was at T₂ (5‰) for<i>M. rosenbergii</i>. Gross return, net profit, and benefit–cost ratio (BCR) analysis revealed higher profit T₄ (15‰) than T₃ (10‰), T₂ (5‰), and T₁ (0‰). TB counts were found to be significantly greater (<i>p</i> &lt; 0.05) in T₄ than other treatments. Hemocyte counts for <i>L. vannamei</i> were significantly higher (<i>p</i> &lt; 0.05) in T₄ (15‰) than T₃ (10‰), T₂ (5‰), and T₁ (0‰) and for <i>M. rosenbergii</i> hemocyte was significantly higher (<i>p</i> &lt; 0.05) in T₁ (0‰) than T₂ (5 ‰), T₃ (10 ‰), and T₄ (15‰). Therefore, it can be suggested that 15‰ salinity will be the best condition for the nursery bi-culture of white leg shrimp (<i>L. vannamei</i>) and giant prawn (<i>M. rosenbergii</i>) in the biofloc system.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5368636","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Different Dried Chlorella pyrenoidosa Preparations on Fatty Acid Profiles and Reproductive Outcomes in Daphnia carinata","authors":"Jiaqi Jin,&nbsp;Feng Zhang,&nbsp;Tian Yang,&nbsp;Yinan Jiang,&nbsp;Wenzhi Wei","doi":"10.1155/are/3917802","DOIUrl":"https://doi.org/10.1155/are/3917802","url":null,"abstract":"<div>\u0000 <p><i>Daphnia carinata</i> is an important biological live feed in aquaculture. Conventionally, <i>D. carinata</i> is cultivated and fed with fresh <i>Chlorella pyrenoidosa</i>, which is inconvenient for storage and transportation. After undergoing vacuum freeze-drying or spray-drying, <i>C. pyrenoidosa</i> exhibits advantages such as reduced weight and easier storage and transport. This study investigated the effects of feeding freeze-dried and spray-dried <i>Chlorella</i> on the reproductive performance and fatty acid composition of <i>D. carinata</i>. The results indicated that there was no significant difference in reproductive performance between the two groups of <i>D. carinata</i> fed with freeze-dried <i>Chlorella</i> and fresh <i>Chlorella</i>. However, a significant decline in reproductive performance was observed in the group fed with spray-dried <i>Chlorella</i>. The contents of crude fat and unsaturated fatty acids in freeze-dried <i>Chlorella</i> were significantly higher than in spray-dried <i>Chlorella</i>. Additionally, <i>D. carinata</i> fed with freeze-dried <i>Chlorella</i> exhibited significantly higher levels of unsaturated fatty acids (especially ALA and EPA) than those fed with spray-dried <i>Chlorella</i>. In conclusion, freeze-dried <i>Chlorella</i> can be used as an alternative to fresh <i>Chlorella</i> for cultivating <i>D. carinata</i>. Unsaturated fatty acids are a key factor influencing the reproductive performance of <i>D. carinata</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3917802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and Comparison Germplasm Resource of Two Species of Macrobrachium in China (M. hainanense and M. nipponense)","authors":"Khadraoui Djamel Eddine,&nbsp;Zijian Gao,&nbsp;Peter Daka,&nbsp;Wenyi Zhang,&nbsp;Sufei Jiang,&nbsp;Yiwei Xiong,&nbsp;Hui Qiao,&nbsp;Hongtuo Fu","doi":"10.1155/are/5531870","DOIUrl":"https://doi.org/10.1155/are/5531870","url":null,"abstract":"<div>\u0000 <p>The <i>Macrobrachium</i> genus thrives in freshwater and brackish environments. <i>M. hainanense</i>, a nocturnal freshwater prawn, is economically significant in China due to its delicious meat and rich nutrition. Its distribution is limited to regions like Zhejiang, Guangdong, Guangxi, Fujian, and Hainan due to breeding habits near estuaries and low-temperature tolerance. Despite its economic importance, there are limited studies on <i>M. hainanense</i>, particularly regarding its germplasm resources. This study investigates the growth traits, nutritional composition, enzyme activity, and genetic diversity and structure of seven <i>M. nipponense</i> populations and three <i>M. hainanense</i> populations to inform sustainable aquaculture and conservation efforts, comparing them with the new hybrid varieties of <i>M. nipponense</i>, which successfully cultivated, Because of its fast growth, high adaptability, and strong reproductive capacity was chosen as a control sample due to its well-established quality, economic value, reputation, ensuring competitive comparison. The amino acids, fatty acids, and physiological and biochemical indicators among <i>M. hainanense</i> populations and <i>M. nipponense</i> populations showed significant differentiation. <i>M. hainanense</i> populations showed minor differences in nutritional components except for total sugar and astaxanthin content that were significantly different, whereas <i>M. nipponense</i> populations showed almost no differences. Essential amino acids in <i>M. hainanense</i> comprised 34.42%–37.53% of the total amino acid content. Differences in glycine, isoleucine, and cystine were notable in <i>M. hainanense</i>, while glycine and proline differed in <i>M. nipponense</i>. Fatty acid components varied among the populations studied. Genetic diversity analysis revealed that the polymorphism information content (PIC) values for <i>M. hainanense</i> were higher than those for <i>M. nipponense</i>. The Changhua River (CH) and Nandu River (ND) populations of <i>M. hainanense</i> had the lowest nucleotide diversity, while the Oujiang River (OU) population had the highest. The OU population also exhibited the greatest genetic diversity, with the lowest inbreeding coefficient, while the CH population had the highest. There was slight genetic differentiation among <i>M. hainanense</i> populations, with geographical isolation and artificial selection contributing to genetic structure differences. This study is the first to examine population-wide genetic variability in <i>M. hainanense</i>, highlighting the need for comprehensive conservation and breeding strategies to maintain and enhance genetic variability in this species. The study concludes that both <i>M. hainanense</i> and <i>M. nipponense</i> have rich protein content and low-fat content, with varied genetic relationships and differentiation among populations.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5531870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Muscle Necrosis in Cultured Penaeus vannamei in Latin America","authors":"Pablo Intriago,&nbsp;Bolivar Montiel,&nbsp;Mauricio Valarezo,&nbsp;Kelly Arteaga,&nbsp;Xavier Romero,&nbsp;Alejandra Montenegro","doi":"10.1155/are/9919229","DOIUrl":"https://doi.org/10.1155/are/9919229","url":null,"abstract":"<div>\u0000 <p>A pathology characterized by multifocal reddish patches on the cuticle of shrimp has been reported by shrimp farmers in Latin America. These red patches are commonly associated with small black necrotic spots and white muscle necrosis. One significant impact of this pathology is the reduction in shrimp prices due to the appearance of the animals before and after cooking. The disease is more prevalent in areas with salinities above 5 ppt, with one farm reporting a 45% incidence in one pond. Histopathological examination revealed infiltration of hemocytes in the muscle tissue, melanosis, muscle necrosis, and the presence of Gram-negative rods in the muscle tissue. Given the presence of bacteria and negative polymerase chain reaction (PCR) results for muscle-infecting viruses such as infectious myonecrosis virus (IMNV) and <i>Penaeus vannamei</i> nodavirus (PvNV), this pathology is likely due to bacterial infection, though environmental stressors cannot be ruled out as contributing factors in this complex condition. Several <i>Vibrio</i> strains were isolated from the affected tissue and identified using API 20E and PCR. Two strains were selected for in vivo experimentation: <i>Vibrio parahaemolyticus</i> (C8Cg, PirAB toxin-positive) and <i>V. alginolyticus</i> (C3Ver, PirAB toxin-negative). Shrimp injected with both live and autoclaved bacteria showed similar histopathological changes, suggesting that the pathology is due to virulence factors within the bacterial cells that can still provoke a strong immune or toxic response even in the absence of live, actively replicating bacteria.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9919229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Efficacy and Immunogenicity of a Nanoencapsulated ECP-Enriched Aeromonas hydrophila Vaccine in Common Carp (Cyprinus carpio)","authors":"Mojtaba Alishahi,&nbsp;Alireza Behroozian,&nbsp;Elham Osroush,&nbsp;Rahim Peyghan","doi":"10.1155/2024/3249381","DOIUrl":"https://doi.org/10.1155/2024/3249381","url":null,"abstract":"<div>\u0000 <p>Recently, there has been increasing attention regarding the potential application of biodegradable nanoparticles (NPs) as adjuvants in injectable vaccines. In this study, encapsulated antigens of <i>Aeromonas hydrophila</i> in alginate/chitosan polymers were used as injectable vaccines in common carp. Formalin-killed bacteria and extracellular products (ECPs) of <i>A. hydrophila</i> were encapsulated via emulsification method. In total, 480 juvenile common carp (32.1 ± 3.64 g, mean ± SD) were randomly divided into eight triplicate groups (20 fish for each replicate). Groups 1–3 were injected with bacterin, ECP, and bacterin + ECP, respectively. Groups 4–6 were injected with encapsulated bacterin, encapsulated ECP, and encapsulated bacterin + ECP, respectively. Groups 7 and 8 as controls were injected with alginate/chitosan and phosphate-buffered saline (PBS), respectively. Blood samples were collected on days 0, 30, and 60 of the experiment. Immunological parameters, including antibody titer against <i>A. hydrophila</i>, serum lysozyme, bactericidal and complement activities, and nitro blue tetrazolium (NBT) reduction, as well as serum globulin and protein levels, were compared among the groups. Hematological parameters (red blood cell [RBC], white blood cell [WBC], hemoglobin [Hb], and hematocrit (Hct) were evaluated too. Remained fish were challenged with severe <i>A. hydrophila</i>, and mortality was recorded for 10 days. Results showed that anti-<i>A. hydrophila</i> antibody titer, survival rate following bacterial challenge, NBT reduction, serum lysozyme activity, globulin, and protein levels were significantly higher in encapsulated bacterin and encapsulated bacterin + ECP groups compared with the control group (<i>p</i>  &lt; 0.05). Serum bactericidal and complement activity showed no significant change in different groups of sampling points. Then, it can be concluded that the encapsulation of bacterin and bacterin + ECP of <i>A. hydrophila</i> using chitosan/alginate NPs enhanced the protection and immune responses in common carp and this method is a promising candidate for a vaccine adjuvant in fish.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3249381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Supplementation With DDGS in Juvenile of Rainbow Trout (Oncorhynchus mykiss): Effects on Growth Parameters, Digestive Physiology, and Gene Expression","authors":"Şükrü Yıldırım,&nbsp;Ebru Yılmaz,&nbsp;Mehmet Güler,&nbsp;Sema Midilli,&nbsp;Cüneyt Suzer,&nbsp;Deniz Çoban","doi":"10.1155/2024/6890227","DOIUrl":"https://doi.org/10.1155/2024/6890227","url":null,"abstract":"<div>\u0000 <p>In this study, the effects of using the distiller’s dried grains with solubles (DDGS) instead of soybean meal on rainbow trout (<i>Oncorhynchus mykiss</i>) were investigated. Four different experimental diets were prepared with isonitrogenous (43%) and isolipidic (19%) values, incorporating DDGS at levels of 0%, 10%, 20%, and 30%. Fish were weighed (18.5–20.2 g) and randomly distributed in 12 tanks (140 L water) with 22 fish in each tank. The feeding trial lasted 60 days and was conducted in triplicate. The best weight gain (WG), specific growth rate (SGR), and feed conversion ratio (FCR) were observed in the DDGS20 experimental group. While viscerosomatic index (VSI) and hepatosomatic index (HSI) values were found to be the highest in the DDGS30 experimental group, the spleen-somatic index (SSI) was found to be the highest in the DDGS10 experimental group (<i>p</i>  &lt; 0.05). It was found that the nutrient digestibility ratio values and fish meat chemical composition values of the experimental diets were similar between the groups (<i>p</i>  &gt; 0.05). Additionally, it was analyzed that the use of DDGS protein sources in diets did not negatively affect the coloration of the experimental fish. While DDGS percentage increased in the diet, hepatocyte nucleus diameter was decreasing (<i>p</i>  &lt; 0.05). In the distal intestine, the DDGS10 and DDGS20 groups gave results that are more likely to be digestible, which depends on villi length. The results of the DDGS30 groups had no negative effects on both liver and distal intestine. The genes interferon gamma (<i>IFN-γ</i>), tumor necrosis factor alpha (<i>TNF-α</i>), interleukin 8 (<i>IL-8</i>), and superoxide dismutase 2 (<i>SOD2</i>) exhibited higher fold changes when DDGS2 contribution was present in the liver, whereas the interleukin 1 beta (<i>IL-1β</i>) gene exhibited lower fold change. Furthermore, the mean fold changes increased with the proportion of the DDGS additive in the immunoglobulin M (<i>IgM</i>) heavy chain and immunoglobulin T (<i>IgT</i>) genes but decreased in the catalase (<i>CAT</i>) and glutathione peroxidase 1 (<i>GPX1</i>) genes. <i>TNF-α</i> and <i>SOD2</i> genes showed the lowest fold changes in the head kidney in the presence of DDGS20, whereas <i>IL-1β</i> and <i>GPX1</i> genes exhibited the highest fold changes in the presence of DDGS10. Moreover, the utilization of feed additives extracted from DDGS in feed formulations resulted in cost reductions, thereby positively influencing the production cost per kilogram of feed. Among the trials, the incorporation of DDGS20 had the most beneficial impact on the cost per kilogram of fish due to its comparatively lower cost.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6890227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Macrobrachium nipponense on Phytoplankton Communities and Water Environmental Factors in Macrobrachium rosenbergii Culture","authors":"Ye Xu,&nbsp;Yaming Feng,&nbsp;Shuanglin Zhang,&nbsp;Zhijing Yang,&nbsp;Wentao Xu,&nbsp;Jinhua Gong,&nbsp;Hailong Gu","doi":"10.1155/are/4218312","DOIUrl":"https://doi.org/10.1155/are/4218312","url":null,"abstract":"<div>\u0000 <p>Excessive feed input led to eutrophication and Cyanobacterial blooms in the culture of giant freshwater prawn (GFP, <i>Macrobrachium rosenbergii</i>). In order to improve the water quality and control the Cyanobacterial blooms, we tried the mixed culture of oriental river prawn (ORP, <i>Macrobrachium nipponense</i>), which can feed on plant and organic debris. In this study, the culture trial began on June 20th and ended on September 10th. The control group (CG) was GFPs monoculture, while the experimental group (EG) cultured GFPs and ORPs. Water samples were collected from the ponds for five times to compare the changes of water environmental factors and phytoplankton communities in CG and EG. Phosphate, nitrite, sulfide, and ammonia nitrogen in CG were significantly higher than those in EG at the end of culture (<i>p</i> &lt; 0.05). In the middle stage of culture, the Margalef index, Pielou index, and Shannon–Wiener index of EG were significantly higher than those of CG (<i>p</i> &lt; 0.05). The biomass and density of phytoplankton in EG were lower than those in CG, and the occurrence of Cyanobacterial blooms in EG was later. The results indicated that ORPs in the mixed culture model effectively transformed and stored part of the energy by consuming the residual bait, which reduced the degree of eutrophication and Cyanobacterial blooms.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4218312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Different Carbon Sources on Water Quality, Physiology, Growth, and Intestinal Microbiota in Cultured Sea Cucumber (Apostichopus japonicus) With Biofloc Technology","authors":"Haoran Xiao,&nbsp;Qi Ye,&nbsp;Zitong Wang,&nbsp;Weiyan Li,&nbsp;Shuchao Ruan,&nbsp;Lingshu Han,&nbsp;Kaihao Zhao,&nbsp;Xiaonan Li,&nbsp;Wanrong Tian,&nbsp;Yaqing Chang,&nbsp;Jun Ding","doi":"10.1155/are/6294937","DOIUrl":"https://doi.org/10.1155/are/6294937","url":null,"abstract":"<div>\u0000 <p>The biofloc technology (BFT) in sea cucumber (<i>Apostichopus japonicus</i>) farming utilizes nutrient cycling, driven by heterotrophic microorganisms, to improve water quality and promote sustainable aquaculture. This study explored the potential of sodium bicarbonate and sodium acetate as novel carbon sources, which may offer advantages in maintaining water stability and enhancing microbial diversity compared to traditional carbon sources. Juvenile sea cucumbers were cultured in a 64-day trial and assigned to five treatment groups: S (sucrose), SSa (S + sodium acetate), SSb (S + sodium bicarbonate), Nc (no carbon source), and Cg (control group), each treatment group was repeated three times (<i>n</i> = 3). The inclusion of sodium bicarbonate and sodium acetate significantly improved water quality parameters. The group SSb exhibited a higher pH (8.28), and significantly lower ammonia nitrogen (0.501 mg L⁻¹), and nitrite levels (0.031 mg L⁻¹) compared to other groups. Additionally, the biofloc volume (BFV; 17 mL L⁻¹), total suspended solids (TSSs; 238 mg L⁻¹), and protein content (13.9%) in the group SSb were all within the tolerance range for sea cucumbers. Group SSb also showed the significantly highest weight gain rate (WGR; 78.5%) and specific growth rate (SGR; 0.91%), alongside increased enzyme activities, including superoxide dismutase (SOD; 179 U mL⁻¹) and peroxidase (POD; 1.59 U mL⁻¹), which were significantly higher than those in other groups. Furthermore, the group SSb demonstrated enhanced gut microbiota diversity, characterized by a higher abundance of beneficial <i>Bacillus</i> and lower levels of harmful <i>Vibrio</i> and <i>Pseudomonas</i>. The addition of SSb significantly improved water quality by maintaining optimal pH and reducing harmful nitrogen compounds, which in turn supported enhanced physiological growth and increased gut microbiota diversity, indicating its potential as a superior carbon source in biofloc systems.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6294937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142868752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Insights Into the Incorporation of Common Hop (Humulus lupulus L.) Into the Fish Feed: Effect on Growth Performance, Whole-Body Proximate Composition, Fillet Fatty Acid Composition, Blood Biochemical, and Antioxidant Properties of Common Carp (Cyprinus carpio L.)","authors":"Faranak Dadras,&nbsp;Hadiseh Dadras,&nbsp;Latifeh Chupani,&nbsp;Josef Velisek,&nbsp;Alzbeta Stara,&nbsp;Ales Tomcala,&nbsp;Eliska Zuskova","doi":"10.1155/are/9290844","DOIUrl":"https://doi.org/10.1155/are/9290844","url":null,"abstract":"<div>\u0000 <p>The present study assessed the effect of common hop (<i>Humulus lupulus</i> L.) extract on common carp (<i>Cyprinus carpio</i> L.). Fish (initial weight of 32.22 ± 0.65 g) were divided into three groups and fed diets containing 0 (control), 0.75 g (H_0.75), and 1.5 g (H_1.5) of common hop extract per kg of isonitrogenous, isolipidic, and isoenergetic commercial diet for 8 weeks. No significant differences in growth performance across treatments were observed (<i>p</i> &gt; 0.05). Protein content in whole-body proximate composition increased significantly in H_1.5 compared to the control (<i>p</i> &lt; 0.05). The H_0.75 showed a significant increase in lipid peroxidation (LPO) and reduced glutathione (GSH) levels compared to the control (<i>p</i>  &lt; 0.05). While H_1.5 had higher acetylcholinesterase (AChE) than group H_0.75 (<i>p</i>  &lt; 0.05), no significant change was observed in AChE, catalase (CAT), and superoxide dismutase (SOD) activities in treated groups compared to the control (<i>p</i>  &gt; 0.05). Blood biochemical indices, alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were significantly lower in H_0.75 and H_1.5 than in the control (<i>p</i> &lt; 0.05), while lactate dehydrogenase (LDH) increased significantly in H_1.5 compared to the control (<i>p</i> &lt; 0.05). Cholesterol, triglyceride (TG), and the number of white blood cells (WBCs) showed no significant differences across treatments (<i>p</i> &gt; 0.05). H_0.75 significantly increased the proportion of polyunsaturated fatty acids (PUFAs) like α-linolenic acid, docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA; <i>p</i> &lt; 0.05), and both H_0.75 and H_1.5 raised considerably the value of oleic acid and eicosatrienoic acid compared with the control (<i>p</i> &lt; 0.05); in contrast, eicosapentaenoic acid (EPA) was decreased significantly by H_1.5 compared to the control (<i>p</i> &lt; 0.05). Supplementation of the common hop at 0.75 and 1.5 g kg⁻¹ positively affected farmed carp.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9290844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142861330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}