Aquaculture Research最新文献

{"title":"Pathogen Screening and Genetic Analysis of Brazilian Black Tiger Shrimp (Penaeus monodon) Populations for Enhancing Aquaculture Stocks","authors":"Thales Passos de Andrade,&nbsp;Amanda Rafaela Cunha Gomes,&nbsp;Francisco Rodrigues Norberto Junior,&nbsp;James Kijas,&nbsp;Melony Sellars,&nbsp;Jeremy Brawner","doi":"10.1155/are/9086659","DOIUrl":"https://doi.org/10.1155/are/9086659","url":null,"abstract":"<div>\u0000 <p>Black tiger prawn (shrimp) (<i>Penaeus monodon</i>) populations that have established in the wild across the north of Brazil provide an alternative for shrimp aquaculture systems that are primarily based on pacific white-leg shrimp (<i>Litopenaeus vannamei</i>). A population of <i>P. monodon</i> that was sourced from the state of Ceará in Brazil, used to evaluate husbandry and breeding systems, and was assessed for pathogen incidence and genotyped to evaluate inbreeding, genetic diversity, and population structure after 10 generations of domestication. Ninety-six <i>P. monodon</i> were screened for 19 pathogens listed by WOAH, MAPA, and other significant pathogens. All 1133 qPCR/RT-PCR tests were negative, and the histopathology of 42 samples showed no significant morphological changes when inspected for general health status. Genotype data from other populations of <i>P. monodon</i> collected and genotyped with the same DNA marker panel were used to estimate inbreeding coefficients and population diversity estimates. The Brazilian population’s inbreeding coefficient estimate was low (0.03) compared to most comparison populations. Genetic distance estimates (<i>F</i>_st) showed Brazilian broodstock were genetically similar to both domesticated populations from Vietnam and wild populations from Australia, while being genetically different to populations originating from the Mozambique Channel region. The absence of pathogens and lack of inbreeding indicate that the population is well adapted to this region, making it suitable for the development of a structured breeding program to provide a reliable domestic source of genetically improved <i>P. monodon</i>. Such a program would contribute significantly to the long-term sustainability, resilience, and productivity of the shrimp industry in north/northeast of Brazil, reducing dependence on imported stocks and enhance local aquaculture development.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9086659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Thermal Sensitivity Varies Among Full Sibling Families of Atlantic Salmon","authors":"Andrew W. Griffith,&nbsp;Erin L. Legacki,&nbsp;Danielle Boyd,&nbsp;Brian C. Peterson","doi":"10.1155/are/3405436","DOIUrl":"https://doi.org/10.1155/are/3405436","url":null,"abstract":"<div>\u0000 <p>Atlantic salmon production constitutes a growing industry in the United States and is largely reliant upon cage-culture in which fish are cultivated within enclosed nets or pens in environmental settings that are prone to natural variation. Temperature fluctuation in particular can substantially inhibit or accelerate production. Here we investigate the larval respiratory capacity (respiratory <i>Q</i>₁₀) among pedigreed families of Atlantic salmon (<i>Salmo salar</i>) spawned in a hatchery facility exposed to acute thermal stress. Individual larvae from full-sibling families were incubated at three temperatures along a 10°C thermal gradient (6, 12, and 16°C) in calibrated respiration chambers and linear declines in oxygen content were used to determine individual respiration rates and family-based <i>Q</i>₁₀. Metabolites from the tricarboxylic acid (TCA) pathway, a key pathway involved in cellular energy supply, were quantified in preserved whole tissue from each larval family using liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify potential biochemical markers associated with thermal sensitivity. Clear family × environment interactions were observed, whereby some larval families exhibited negative rate-increases with warmer environments as opposed to others that exhibited enhanced respiration rates. Neither total metabolite content nor the abundance of single metabolite(s) were associated with family-specific trends in respiration rate with temperature. Our findings highlight the use for identifying contrasting phenotypes among pedigreed families of Atlantic salmon and their potential in selective breeding programs for tolerance to dynamic environmental conditions.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3405436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Transcriptomic Profile of Mucosal Immune Responses in the Intestine of Japanese Puffer (Takifugu rubripes) Infected With Vibrio harveyi","authors":"Yihao Li,&nbsp;Hongli Ma,&nbsp;Zhixin Guo,&nbsp;Xiaocen Lu,&nbsp;Peilin Zhou,&nbsp;Yanhong Cui,&nbsp;Shigen Ye","doi":"10.1155/are/9921481","DOIUrl":"https://doi.org/10.1155/are/9921481","url":null,"abstract":"<div>\u0000 <p>Japanese puffer (<i>Takifugu rubripes</i>) is an important mariculture species, but the development of the aquaculture industry has been accompanied by serious disease problems. <i>Vibrio harveyi</i> is one of the most important pathogens in <i>T. rubripes</i>. To gain a more comprehensive understanding of the molecular mechanism responsible for intestinal mucosal immunity of <i>T. rubripes</i> during <i>V. harveyi</i> infection, we established a transcriptome of the posterior intestine of <i>T. rubripes</i> after 0, 3, and 24 h of infection with <i>V. harveyi</i>. 12 fish (weighing 300 ± 5.5 g) were randomly selected from 60 <i>T. rubripes</i> and four were assigned to each time point. Transcriptomic analysis revealed 3,042 and 3,342 differentially expressed genes (DEGs) at 3 h and 24 h, respectively, compared with those at 0 h. Enrichment analysis of these DEGs revealed significant mucosal immune-related pathways, including cytokine–cytokine receptor interaction, phagosome, and cell adhesion molecules (CAMs) signaling pathways. In addition, the intestinal histopathological changes were explored after <i>V. harveyi</i> infection. Further analysis showed that the structure of infected intestines was still integrated, with the majority of the pathological trauma occurring in the submucosa. Our results provided new insights for further study of the mucosal immune defense mechanisms of fish against <i>V. harveyi</i>, promoting our understanding of this severe disease.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9921481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catch-Based Stock Assessment of Silver Croaker (Pennahia argentata) Based on Different Fishing Indicators in the East China Sea","authors":"Shiqing Zhao,&nbsp;Richard Kindong,&nbsp;Chunxia Gao,&nbsp;Zhiwei Liu,&nbsp;Siquan Tian","doi":"10.1155/are/1916662","DOIUrl":"https://doi.org/10.1155/are/1916662","url":null,"abstract":"<div>\u0000 <p>Data limitations in multispecies fisheries make traditional stock assessments challenging for many developing coastal countries. Despite China’s large marine ecosystems, most fish stocks remain unassessed. This study evaluates the stock status of silver croaker (<i>Pennahia argentata</i>) in the East China Sea (ECS) from 2003 to 2022 using catch-based methods and data from the China Fisheries Statistical Yearbook (CFSY). Three models indicate that the 2022 catch is below the estimated maximum sustainable yield (MSY), suggesting a healthy stock status. The study also tests four catch per unit effort (CPUE) indicators, finding that combining CPUE2 and CPUE4 in the just another Bayesian biomass assessment (JABBA) model yields the best fit. Sensitivity analysis highlights the importance of initial biomass settings in JABBA. Given the limitations of catch-based models like catch-MSY plus plus (CMSY++) and the absence of survey data, a more conservative management approach is recommended. Additionally, the catch-to-trawl vessel horsepower ratio is suggested as a reliable CPUE indicator. These findings support more accurate assessments and sustainable management of silver croaker in the ECS.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1916662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and Characterization of Adiponectin Receptors in Rana amurensis During Aeromonas hydrophila Infection","authors":"Yugang Sun,&nbsp;Dongmei Xu,&nbsp;Jinhan A,&nbsp;Huimin Ren,&nbsp;Hina Hassan,&nbsp;Yufen Liu,&nbsp;Peng Liu,&nbsp;Wenge Zhao","doi":"10.1155/are/3562469","DOIUrl":"https://doi.org/10.1155/are/3562469","url":null,"abstract":"<div>\u0000 <p>This study describes the cloning and characterization of adiponectin receptors (<i>AdipoR1</i> and <i>AdipoR2</i>), as well as the analysis of their mRNA and protein expression in <i>Rana amurensis</i> at different time points after <i>Aeromonas hydrophila</i> (<i>Ah</i>) infection. The open reading frame (ORF) sequences of <i>AdipoR1</i> (1137 bp) and <i>AdipoR2</i> (1164 bp) were successfully identified. The deduced amino acid sequences of <i>AdipoR1</i> and <i>AdipoR2</i> exhibited a closer genetic affinity to amphibians such as <i>Rana temporaria</i> and <i>Bufo gargarizan</i>s than to nonamphibian species like <i>Mus musculus</i> and <i>Danio rerio</i>. For uninfected, clinically healthy <i>R. amurensis</i>, the expression levels of <i>AdipoR1</i> and <i>AdipoR2</i> mRNA was significantly higher in the kidney, muscle, and stomach (<i>p</i> &lt; 0.05). Under <i>Ah</i> stress, the relative expression levels of <i>AdipoR1</i> mRNA in the liver, lung, muscle, and stomach peaked at 16 h postinfection; however, the <i>AdipoR2</i> mRNA in the lung, muscle, and stomach reached the maximum at 72 h. Our study suggests that <i>AdipoR1</i> and <i>AdipoR2</i> were localized on the surface of the cell membrane; at the same time, the expression of <i>AdipoR1</i> and <i>AdipoR2</i> in <i>R. amurensis</i> varied across different time points and tissues during <i>Ah</i> infection. This study is the first to investigate the <i>AdipoRs</i> genes of <i>R. amurensis</i> in cold high-latitude areas of China. It highlights the importance of <i>AdipoR1</i> and <i>AdipoR2</i> immune function and provides valuable insights for the conservation and sustainable utilization of <i>R. amurensis</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3562469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Bacteriophage on Antibacterial Properties, Nonspecific Immune Responses, and Gut Microbiota in Litopenaeus vannamei Post Vibrio parahaemolyticus Infection","authors":"Dongdong Song,&nbsp;Baojun Shi,&nbsp;Jinlu Huang,&nbsp;Jian Wang","doi":"10.1155/are/7731145","DOIUrl":"https://doi.org/10.1155/are/7731145","url":null,"abstract":"<div>\u0000 <p>Effective immune regulation and balanced gut microbiota play important roles in preventing pathogen infections in <i>Litopenaeus vannamei</i> farming. Bacteriophages are a promising candidate in pathogen control for their specific antibacterial properties. While previous studies focused on the direct antibacterial effects of phages, their effects on nonspecific immune responses and gut microbiota after infection remains to be less explored. In this study, a lytic <i>Vibrio parahaemolyticus</i> phage was isolated from wastewater with a broad host range (66.7% lytic efficiency), low multiplicity of infection (MOI; 0.1), and high environmental tolerance (pH: 3–11; temperature: 4–60°C). Whole genome analysis revealed a 93,814 bp double-stranded linear DNA molecule with 45.1% GC. Both the in vitro cocultivation (24 h) and in vivo shrimp cultivation trails (7 days) demonstrated that phage could effectively reduce the quantities of <i>Vibrio</i> (&gt;99%). The in vivo phage fed shrimp exhibited elevated levels of nonspecific immune-related enzymes like alkaline phosphatase (AKP), catalase (CAT), superoxide dismutase (SOD), phenoloxidase (PO), and lysozyme (LZM) and upregulated immune-related gene expression including those of antimicrobial peptides, pattern recognition receptors (PRRs), and pattern recognition proteins. Additionally, phage treatment improved the diversity of the gut microbiota (Shannon-10 index) after <i>Vibrio</i> infection, indicating restored microbial balance in shrimp. These results suggest that phage therapy promotes nonspecific immune responses and repair intestinal dysbacteriosis in shrimp after <i>Vibrio</i> infection, elucidating a promising strategy to treat pathogenic <i>Vibrio</i> in shrimp aquaculture.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7731145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial Replacement of Fish Meal With Shrimp Waste Meal: Effects on Growth, Digestibility, and Immunity in Juvenile Beluga Sturgeon (Huso huso)","authors":"Amirsoheil Taheri,&nbsp;Abdolmohammad Abedian Kenari,&nbsp;Maryam Aftabgard","doi":"10.1155/are/5469830","DOIUrl":"https://doi.org/10.1155/are/5469830","url":null,"abstract":"<div>\u0000 <p>This study aimed to investigate the effects of replacing different levels of dietary shrimp waste meal (SWM) with fish meal (FM) on growth performance (GP), carcass composition (CC), apparent digestibility (AD), and innate immunity of juvenile beluga sturgeon (<i>Huso huso</i>). A total of 180 juvenile beluga with an average weight of 130.0 ± 6.5 g were assigned to five treatments (with three replicates each): SWM0 (control group), SWM5, SWM10, SWM15, and SWM20, corresponding to 0%, 5%, 10%, 15%, and 20% inclusion of SWM in place of FM in their diets for 8 weeks. The results showed that GP and protein AD declined in the SWM5 group (<i>p</i> &lt; 0.05), while no significant differences were observed in GP and AD across the other treatments compared to the control (<i>p</i> &gt; 0.05). Regarding CC, no significant differences were found among the treatments. The fatty acid (FA) profile of the muscles in juvenile beluga fed with SWM5–20 was similar to that of the control group, with the only notable difference being a decrease in eicosapentaenoic acid (EPA) as dietary SWM levels increased (<i>p</i> &lt; 0.05). The essential amino acid (EAA) content also decreased in the SWM20 group (<i>p</i> &lt; 0.05), although the EAA-to-nonessential amino acid (NEAA) ratio in the SWM5–20 treatments was not significantly different from that of the control group (<i>p</i> &gt; 0.05). The highest levels of serum lysozyme (LYZ) and alternative complement hemolytic (ACH50) activity were observed in the SWM15 group, with the lowest levels found in the control group (<i>p</i> &lt; 0.05). The results showed that substituting FM with SWM at a rate of 15%–20% in the diet had no significant and negative impact on GP, nutritional value, or digestibility. On the contrary, it boosted the immune system of juvenile beluga sturgeon (<i>H. huso</i>). The positive immunostimulation appears to be related to the beneficial effects of amino acid (AA) or imino acids (IAs) and chitin as well as their recognition by mannose/fucose receptors attached to white blood cells.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5469830","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Acute and Long-Term Hypoxia and Hyperoxia on Antioxidant Metabolism and Gene Expression in Juvenile Rainbow Trout (Oncorhynchus mykiss)","authors":"Abdullah Tunç,&nbsp;Orhan Erdoğan,&nbsp;Onur Vural,&nbsp;Ercüment Aksakal","doi":"10.1155/are/6628284","DOIUrl":"https://doi.org/10.1155/are/6628284","url":null,"abstract":"<div>\u0000 <p>Aquatic organisms experience oxidative stress due to environmental stressors that promote oxygen exchange, such as eutrophication, algal photosynthetic activity, or changes in water temperature. This study aimed to clarify the effects of oxidative stress on the liver of juvenile rainbow trout (<i>Oncorhynchus mykiss</i>) exposed to hypoxia and hyperoxia by examining the physiological roles of hydrogen peroxide, glutathione (GSH) redox status, malondialdehyde (MDA) concentration, antioxidant enzyme activity, and antioxidant gene expressions. Dissolved oxygen levels were maintained at 4.0 ± 0.5 mg/L for hypoxia, 7.5 ± 0.5 mg/L for normoxia, and 12 ± 1.2 mg/L for hyperoxia. Liver samples were collected from each experimental group following exposure (6, 12, 24, 48, and 72 h) and chronic exposure (28 days). Under both hypoxia and hyperoxia conditions, reduced GSH levels and the oxidative stress index (OSI) decreased compared to normoxia (control group), whereas oxidized glutathione (GSSG) levels, the GSH/GSSG ratio, and MDA concentrations increased. Hydrogen peroxide levels were unstable. Superoxide dismutase (SOD) activity remained consistently lower than that of the control group. Catalase (CAT) activity decreased with chronic exposure to hyperoxia. Glutathione peroxidase (GPx) activity fluctuated with varying oxygen treatments. Glutathione-S-transferase (GST) activity increased after 12 h of hypoxia exposure but decreased with acute hyperoxia; however, chronic hyperoxia exposure eventually increased GST activity. Glutathione reductase (GR) activity is generally reduced under hypoxia. The expressions of SOD and CAT increased at 24 h but decreased at other times. GPx expression increased under chronic hypoxia but decreased under hyperoxia, while GST expression decreased with chronic treatments. Hypoxia and hyperoxia influence the antioxidant defense system in the fish liver through different pathways. While a coordinated relationship between gene expression and enzyme activities is observed under acute exposures, this coordination diminishes during chronic exposures, leading to the depletion of defense mechanisms. This suggests that the capacity of aquatic organisms to adapt to oxidative stress is limited and that post-transcriptional mechanisms play a significant role in regulating antioxidant responses.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6628284","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Analysis of the Growth Performance, Morphological Indicators, and Transcriptome Between Two Coastal Regions of China in the Mud Crab (Scylla paramamosain)","authors":"Yue Wang,&nbsp;Xiao-Shan Wang,&nbsp;Sheng-Yu Liu,&nbsp;Ben-Jian Wang,&nbsp;Zhi-Xing Su,&nbsp;Xiao-Kang Lv,&nbsp;Jia-Yuan Xu,&nbsp;Xue-Feng Song,&nbsp;Bian-Bian Zhang,&nbsp;Li-Guo Yang","doi":"10.1155/are/6508890","DOIUrl":"https://doi.org/10.1155/are/6508890","url":null,"abstract":"<div>\u0000 <p>The mud crab (<i>Scylla paramamosain</i>), a commercially important species in China, is widely distributed along the southeastern coast. This study compares the growth performance, morphological traits, and transcriptomic profiles of two geographically distinct populations from Sanmen County, Zhejiang (SMC) and Zhangpu County, Fujian (ZPC). Juvenile crabs collected from both regions were reared in ponds under identical conditions for 7 months. Key results showed that the SMC population exhibited significantly higher daily weight gain, specific growth rate (SGR), and propodus-to-cheliped length ratio compared to ZPC (<i>p</i> &lt; 0.01 for both sexes). Transcriptomic analysis revealed 44 consistently upregulated differentially expressed genes (DEGs) in SMC individuals. Among these, <i>Otop</i> (Otopetrin 1), <i>Smyd</i> (SET and MYND domain containing), <i>AM1159</i> (<i>Cancer pagurus</i> Cuticle protein AM1159), <i>PPAF1</i> (PPAR gamma coactivator 1 <i>α</i>), and <i>NARG2</i> (NMDA receptor regulated 2) were functionally linked to molting and growth regulation in crustaceans. Notably, two mTOR pathway-associated genes (<i>QDPR</i> [Quinoid dihydropteridine reductase] and <i>NLRC 3</i> [NLR family CARD domain containing 3]) and several energy/nutrient metabolism genes were also upregulated in SMC. Enrichment analysis identified four active KEGG pathways in SMC: “Melanogenesis,” “Riboflavin metabolism,” “Choline metabolism,” and “Ribosome biogenesis.” These findings suggest that the superior growth performance of SMC crabs may stem from enhanced molting efficiency and nutrient allocation mechanisms. While <i>QDPR</i> and <i>NLRC3</i> are hypothesized to mediate mTOR-driven metabolic regulation, further functional validation is required to establish their causative roles. This study provides molecular markers (<i>Otop</i>, <i>Smyd</i>, etc.) for selective breeding programs and a transcriptomic framework for understanding regional adaptation in marine crustaceans.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6508890","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detailed Early Life History of the Giant Muricid Snail Chicoreus ramosus in the Southern China Sea","authors":"Gan Wang,&nbsp;Yu Zhong,&nbsp;Yanping Qin,&nbsp;Qishuai Wang,&nbsp;Jingyue Huang,&nbsp;Chao Yue,&nbsp;Weitao Wan,&nbsp;Haitao Ma,&nbsp;Jun Li,&nbsp;Xiangyong Yu,&nbsp;Yuehuan Zhang","doi":"10.1155/are/4342005","DOIUrl":"https://doi.org/10.1155/are/4342005","url":null,"abstract":"<div>\u0000 <p>Tropical marine life is highly diverse, but the development and utilization of many species remain insufficient. The giant muricid snail <i>Chicoreus ramosus</i>, notable for its large size and high-quality meat, holds significant potential for aquaculture. Previous descriptions of its seedling production were incomplete and sometimes inaccurate. This study details the early life history and aquaculture potential of <i>C. ramosus</i>. Experiments conducted from December 2023 to October 2024 covered parental cultivation, spawning, and developmental tracking. Key findings include female parents spawning 191–531 egg capsules per event, each containing 189–520 fertilized eggs, with up to three spawnings and decreasing capsule numbers in subsequent events. The early life stages are: (1) Inner egg capsule development period: Fertilized eggs progress through stages including polar body release, two-cell, four-cell, eight-cell, morula, blastocyst, gastrula, and veliger larvae stages; (2) Outer egg capsule development: Postveliger larvae emerge and transition to juvenile and youth stages. Specifically, eggs develop into middle veliger larvae in about 4 weeks, followed by a 1–2 week postveliger stage, juvenile metamorphosis around 2 weeks later, and growth into the youth stage in 2–3 weeks. This study provides a successful framework for artificial incubation and seedling production, advancing the potential aquaculture applications of <i>C. ramosus</i>.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4342005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}