Aquaculture Research最新文献

{"title":"Effect of Dietary Arachidonic Acid on the Broodstock Productive Performance and Egg Quality of Largemouth Bass (Micropterus nigricans)","authors":"Hanlin Xu,&nbsp;Biao Yun,&nbsp;Zexia Gao,&nbsp;Jian Wang","doi":"10.1155/are/3091729","DOIUrl":"https://doi.org/10.1155/are/3091729","url":null,"abstract":"<div>\u0000 <p>The influence of dietary arachidonic acid (ARA) on the reproductive performance of broodstock fish is a critical area of aquaculture research. This study investigates the impact of ARA levels in broodstock diets on broodstock hormone, fecundity, egg quality, and larval survival of largemouth bass (<i>Micropterus nigricans</i>). Experimental diets containing 0.9%, 2.0%, and 3.6% ARA as a percentage of total fatty acids (TFAs) were formulated and administered to broodstock over 84 days. Results indicated that a dietary ARA level of 3.6% significantly increased vitellogenin content in broodstock serum while reducing serum estradiol levels. Despite this hormonal shift, reproductive performance metrics, including fecundity, egg hatch rates, and the number of viable eggs until mouth opening, were not significantly affected by the ARA content in the broodstock diet. However, the 3.6% ARA group showed notable improvements in egg size and higher concentrations of ARA and docosahexaenoic acid in the eggs. Based on these results, 0.9% ARA in TFA content in broodstock diet could be sufficient for the reproduction of largemouth bass. A level of 3.6% ARA of TFAs can be added to the diet of largemouth bass broodstock to enhance offspring potential growth performance and healthy status.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3091729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Analysis of Embryonic Development of Embryos From the Crossing of Two Species of Siluriform","authors":"Yasmin Santos dos Reis,&nbsp;Stella Indira Rocha Lobato,&nbsp;Laicia Carneiro-Leite,&nbsp;Lais Pedroso Borges,&nbsp;Lorena Pacheco da Silva,&nbsp;Rodrigo Yutaka Dichoff Kasai,&nbsp;Rosicleire Veríssimo-Silveira,&nbsp;Alexandre Ninhaus-Silveira","doi":"10.1155/are/5566265","DOIUrl":"https://doi.org/10.1155/are/5566265","url":null,"abstract":"<div>\u0000 <p>Based on the need for knowledge about the biology of hybrid fish specimens currently being produced by commercial fish farmers, this study aimed to study the initial ontogenesis up to the fifth day after larval hatching of the Hybrid LP produced by crossing <i>Leiarius marmoratus</i> (♀) and <i>Pseudoplatystoma reticulatum</i> (♂), thus producing important data for fish farming, as well as for ichthyoplankton studies. The embryogenesis of the Hybrid LP was typical of teleosts but with differences and similarities in relation to other species and their parents, such as duration of development and moment of mesoderm segmentation, among others. Five stages of embryonic development were defined, from the moment of fertilization until larval hatching: zygote, cleavage, gastrulation, organogenesis, and hatching. The embryogenesis period was 13 h, at an average temperature of 27.98°C. The embryonic development stages established for the hybrid were like those of its parental species <i>L. marmoratus</i> and <i>P. reticulatum</i>. Thus, through this research, it was possible to conclude that the embryonic development of the Hybrid LP originating from interspecific crosses between females of <i>L. marmoratus</i> with males of <i>P. reticulatum</i> occurred in a development period similar to that found in studies of its parental species as well as for the inverse hybrid (PL), originating from females of <i>P. reticulatum</i> with males of <i>L. marmoratus</i>, under similar temperatures, not being possible to morphologically differentiate the embryos and larvae until the fifth day after hatching, neither of the parental species nor the Hybrid PL.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/5566265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity and Nutritional Quality Comparison in Schizothorax waltoni, Schizothorax o’connori, and Their Different Maternal Natural Hybrids","authors":"Bin Xu,&nbsp;Liang Peng,&nbsp;Tianyi Zhao,&nbsp;Benhe Zeng,&nbsp;Liqiao Zhong,&nbsp;Huke He,&nbsp;Yali Yu,&nbsp;Kaijin Wei,&nbsp;Baoshan Ma","doi":"10.1155/are/8812358","DOIUrl":"https://doi.org/10.1155/are/8812358","url":null,"abstract":"<div>\u0000 <p>Natural hybridization plays an important role in species evolution, and its heterosis also provides inspiration for breeding. The present study is to compare the genetic diversity and muscle nutritional quality of <i>Schizothorax waltoni</i>, <i>Schizothorax o’connori</i>, and their natural hybrids that are naturally distributed in the Yarlung Zangbo River. Based on the cluster analysis, the genetic relationship between 13 hybrids (namely <i>S. w. ♀</i> hybrid) and <i>S. waltoni</i> was found to be closely related, whereas 24 hybrids (namely <i>S. o. ♀</i> hybrid) were closely related to <i>S. o’connori</i>. The haplotype polymorphisms across the four populations varied between 0.847 and 0.936, while the nucleotide polymorphisms were 0.00305–0.00418. The genetic diversity of the two hybrids was higher than that of <i>S. waltoni</i> and <i>S. o’connori</i>. The crude fat content of the two natural hybrids is significantly higher than that of <i>S. waltoni</i> and <i>S. o’connori</i>. The total amino acid (TAA), essential amino acid (EAA), and delicious amino acid (DAA) contents and the EAA index (EAAI) in the two hybrids were greater than those in <i>S. waltoni</i> and <i>S. o’connori</i>. Compare to <i>S. waltoni</i> (22) and <i>S. o’connori</i> (18), the two natural hybrids (both 24) showed the richer types of fatty acids. The highest contents of polyunsaturated fatty acids (PUFAs), <i>ω</i>-3 PUFAs, and <i>ω</i>-6 PUFAs were observed in <i>S. waltoni</i>, and <i>ω</i>-6 PUFA within the two hybrids possessed intermediate characteristics. In conclusion, the hybrids of <i>S. waltoni</i> and <i>S. o’connori</i> showed advantages of growth rate, genetic diversity, and nutrition quality, therefore appearing to have a potential of breeding for further development and utilization.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8812358","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Sequencing and Functional Analysis of Klebsiella Strain mr-1S Originating From Macrobrachium rosenbergii","authors":"Yang Xu,&nbsp;Ying Zhao,&nbsp;Haihua Cheng,&nbsp;Fei Peng,&nbsp;Qiang Gao","doi":"10.1155/are/3407093","DOIUrl":"https://doi.org/10.1155/are/3407093","url":null,"abstract":"<div>\u0000 <p><b>Background:</b><i>Klebsiella</i> mr-1S, a newly emerging pathogen that poses a substantial threat to public health by inducing severe infectious diseases, has been detected in <i>Macrobrachium rosenbergii</i> for the first time in our prior investigation. Given its potential for environmental adaptation and genomic evolution, this study aims to delve into these aspects to better understand its pathogenicity and evolutionary trajectory.</p>\u0000 <p><b>Methods:</b> In this study, the <i>Klebsiella</i> isolates were subjected to routine cultivation in a tryptic soya broth (TSB) medium. Subsequently, the genomic DNA of each isolate was individually extracted and analyzed. Prior to genomic sequencing, the integrity and concentration of the DNA samples were meticulously evaluated to ensure the accuracy and reliability of the subsequent sequencing process.</p>\u0000 <p><b>Results:</b> The genomic sequence of <i>Klebsiella</i> mr-1S was deciphered, revealing a length of 5,143,806 base pairs with a GC content of 54.97%. Remarkably, the genome encompasses a multitude of putative mobile genetic elements (MGEs), including 43 genomic islands (GIs) and 2 prophages. These elements confer upon the bacterium crucial adaptive attributes such as resistance, virulence, and metabolic capabilities. Notably, the identification of prophage-associated clusters originating from the genus Pseudomonas suggests a potential horizontal gene transfer (HGT) mechanism mediated by phages within Pseudomonas, highlighting the complex genetic interactions between different bacterial species.</p>\u0000 <p><b>Conclusion:</b> In addition, the presence of two genes encoding CRISPR-Cas proteins within the <i>Klebsiella</i> mr-1S genome indicates the existence of a functional CRISPR-Cas system in this bacterium. This finding implies that during its evolutionary history, Klebsiella mr-1S may have developed mechanisms to evade host immune recognition, thereby facilitating efficient HGT and enhancing its adaptability and survival capabilities in diverse environments. Overall, this study provides novel insights into the environmental adaptability and genomic plasticity of <i>Klebsiella</i> from <i>Macrobrachium rosenbergii</i>, underscoring its potential as an emerging pathogen in aquatic ecosystems and paving the way for future research on its pathogenic mechanisms and potential control strategies.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/3407093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastic Pollution in Indigenous Fish From the Padma River, Bangladesh: A Case Study","authors":"Md. Tarek Mondal,&nbsp;Md. Aslam Khan,&nbsp;M. Golam Mortuza","doi":"10.1155/are/9917204","DOIUrl":"https://doi.org/10.1155/are/9917204","url":null,"abstract":"<div>\u0000 <p>Microplastics (MPs) are prevalent in aquatic ecosystems; however, inland water bodies have received relatively little attention. These ecosystems provide essential habitat for various fish species, which contribute to ecological equilibrium and support the livelihoods and food security of millions. Fish demonstrate increased vulnerability to microplastic ingestion, making them essential bioindicators of ecosystem health and contamination levels. This study evaluates the presence of MPs in the intestine, gills, and muscle tissue of 30 individuals belonging to five commonly found fish species (<i>Rhinomugil corsula</i>, <i>Mystus tengara</i>, <i>Ompok pabda</i>, <i>Labeo bata</i>, and <i>Cirrhinus mrigala</i>) in the Padma River. A total of 252 MP items of different shapes were recorded in 100% of individuals investigated, exhibiting an average frequency of 8.27 ± 2.16 items per individual. Among the fish species, <i>Ompok pabda</i> exhibited the highest ingestion of MPs, with an average of 11.5 ± 1.87 items per individual, followed by other species. Organ-wise analysis of MPs trend was found to be: intestine &gt; gill &gt; muscle. Fiber was recognized as the most commonly consumed MP type (72%), followed by fragment (22%), pellet (4%), and foam (2%). The white-color MP (24%) was predominant, followed by other colored MPs. The majority of MP particles were found in the size class 1–5 mm (41.10% ± 12.34%), followed by &lt;500 μm (34.18% ± 16.34%) and 500 μm–1 mm (24.72% ± 11.51%). Fourier transform infrared (FT-IR) spectroscopy investigation confirms both high- and low-density polyethylene polymeric compositions of MPs. The study assesses the prevalence of microplastics in Padma River fish, highlighting the immediate necessity for focused conservation initiatives, ongoing monitoring, and evaluation of microplastic pollution to protect aquatic ecosystems and human health.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/9917204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Coexposure Effects of Pyrogallol and Microplastic on the Red Swamp Crayfish Procambarus clarkii","authors":"Rashad E. M. Said,&nbsp;Mohamed Hamed,&nbsp;Walaa M. Shaalan,&nbsp;Heba Allah M. Elbaghdady,&nbsp;Alaa El-Din H. Sayed","doi":"10.1155/are/6084150","DOIUrl":"https://doi.org/10.1155/are/6084150","url":null,"abstract":"<div>\u0000 <p>Investigating the impact of two or more toxic chemicals on aquatic organisms is one of the main objectives of toxicological research. Microplastics (MPs) are identified as a carrier or vector for other pollutants. Therefore, the current study investigated the effects of both pyrogallol (PG) and MPs, individually and in combination, on freshwater crayfish (<i>Procambarus clarkii</i>). Following a 15-day exposure to 10 mg/L of PG and100 mg/L MPs and their combination (10 mg/L PG + 100 mg/L MPs), the hemocyte count, hepatopancreatic parameters, antioxidant variables, and histopathological markers were all assessed in the crayfish. The results revealed that PG and MPs potentially individually or combined altered the hemocyte count (including granular and semigranular) compared to control group (0.001 ≥ <i>p</i> &lt; 0.0001). The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and protein showed significant elevation (<i>p</i> &lt; 0.05) in PG–MP-exposed fish compared to control ones. In comparison to control <i>P. clarkii</i>, the measured oxidation indicators, superoxide dismutase (SOD), glutathione (GSH), and total antioxidant capacity (TAC), showed a significant reduction after single exposure to PG and MPs (0.001 &gt; <i>p</i> &lt; 0.0001), while catalase (CAT) and malondialdehyde (MDA) elevated drastically (0.05 &gt; <i>p</i> &lt; 0.0001). The histology of the hepatopancreas has shown many deformities and abnormalities after individual or combined toxicity, such as vacuolations, degraded hepatopancreatic tubules, eosinophilic deposits, hemocytic infiltrations, and aberrant tubules. Accordingly, there is a synergistic relationship between PG and MP toxicity to crayfish. Therefore, it is crucial to routinely check and assess the discharge of waste into waterways.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6084150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Protective Immunological Effects of Pandanus tectorius Leaf Extract Against Pathogenic Vibrio campbellii Using Gnotobiotic Brine Shrimp Model System","authors":"Patabandi PPSK,&nbsp;Mohamad Asmidar,&nbsp;Yosie Andriani,&nbsp;Parisa Norouzitallab,&nbsp;Yeong Yik Sung,&nbsp;Kartik Baruah","doi":"10.1155/are/8215825","DOIUrl":"https://doi.org/10.1155/are/8215825","url":null,"abstract":"<div>\u0000 <p>This study investigated the protective effects of <i>Pandanus tectorius</i> leaf extract (PLE) on the tolerance of the brine shrimp <i>Artemia franciscana</i> against the pathogen <i>Vibrio campbellii</i>. Axenic <i>Artemia</i> nauplii were hatched and exposed to PLE for 2 h, after which their resistance to <i>V. campbellii</i> was examined. Protective responses were evaluated by measuring nauplii survival and changes in the expression of key immune-related genes, such as heat shock protein 70 (<i>hsp70</i>), <i>hsp60</i>, <i>hsp90</i>, high mobility group box 1 (<i>hmgb1</i>), prophenoloxidase (<i>proPO</i>), and transglutaminase (<i>tgase</i>) upon <i>V. campbellii</i> challenge. Additionally, a <i>Vibrio</i> colonization assay was conducted to assess PLE’s antimicrobial potential. The results revealed that 2 h pretreatment with PLE at a concentration of 1 g/L significantly improved survival against <i>V. campbellii</i>. Immune-related gene expression was induced at different time points (e.g., 12–24 h) during the <i>Vibrio</i> challenge and the colonization assay confirmed PLE’s antimicrobial properties. The observed protection could likely be due to a combination of immune gene activation, antioxidant activity, and the antimicrobial effect of the extract. This study highlights the significance of PLE by indicating its potential to serve as a protective agent in aquaculture, enhancing the resilience of aquatic organisms against biotic stressors like vibrios.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/8215825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143836026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and Morphology Analysis Among the F1 Hybrid Chinese Soft-Shelled Turtle (Pelodiscus sinensis Huangsha ♂×Pelodiscus sinensis Japanese Strains ♀) and the Parental Varieties","authors":"Yixin Liang,&nbsp;Pei Wang,&nbsp;Hewei Xiao,&nbsp;Lizhong Jin,&nbsp;Changqing Huang,&nbsp;Qin Qin,&nbsp;Shuting Xiong,&nbsp;Fanjian Meng,&nbsp;Xiaoqing Wang,&nbsp;Yazhou Hu","doi":"10.1155/are/7076743","DOIUrl":"https://doi.org/10.1155/are/7076743","url":null,"abstract":"<div>\u0000 <p>Hybridization is an effective method for the genetic improvement of breeding varieties, while establishing standardized management systems and identification methods is an important foundation for obtaining stable hybrid new varieties with desirable traits. This study conducted measurements and analyses of eight morphological indicators of three Chinese soft-shelled turtle (<i>Pelodiscus sinensis</i>) populations: the Huangsha turtle (<i>P. sinensis</i> Huangsha, HS), Japanese turtle (<i>P</i>. <i>sinensis</i> Japanese strains, JP), and their hybrid F1 generation (HS ♂ × JP ♀, HJ). Based on several important indicators, a discriminant function was established, and after cross-validation, the comprehensive discriminant accuracy was 86.4%. In addition, we used super-genotyping-by-sequencing (GBS) sequencing technology to analyze the genetic structure and genetic diversity of the three populations. The results showed that the HJ population had a closer genetic relationship with the JP population as the maternal parent and a farther genetic relationship with the HS population. The genetic diversity was between the HS and JP populations, and the genetic distance between the HS and JP populations was far. Through fingerprint analysis technology, core SNP markers for germplasm identification were screened, and a total of 148 core SNP marker loci were obtained. One SNP marker that could distinguish the HJ population from the HS population was developed. This study will provide reference for the hybrid breeding and genetic management of Chinese soft-shelled turtle.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/7076743","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm Quality, Milt Biochemistry, and Cellular Ultrastructure in Fresh and Cryopreserved Sperm of Brycon amazonicus","authors":"Owens José Barros-Barrios,&nbsp;Roger Oswaldo Suárez-Martínez,&nbsp;Edwin Gómez-Ramírez,&nbsp;Diana Nathalie Guaje-Ramírez,&nbsp;Víctor Mauricio Medina-Robles","doi":"10.1155/are/6671651","DOIUrl":"https://doi.org/10.1155/are/6671651","url":null,"abstract":"<div>\u0000 <p>Milt cryopreservation facilitates native fish’s constant production of commercial interest. However, it can damage spermatozoa (sptz) due to the osmotic stress of diluents and low temperatures. This study compares spermatic quality in fresh (SF) and cryopreserved (CS) milt in <i>B. amazonicus</i> for 24 h, 1 month, and 3 months. Eight sexually mature males were induced with carp hypophysis extract. Milt was diluted in one proportion in a glucose solution (5.5%), egg yolk (12%), and dimethyl sulfoxide (10%). An evaluation of parameters such as motility, viability, morphology, total antioxidant capacity (TAC), and adenosine triphosphate (ATP) contents was performed at 24 h, 1 month, and 3 months, and the fertility test was conducted at 24 months of cryopreservation. The motility percentage was higher (<i>p</i> ≤ 0.05) in SF (95.5 ± 2.9%). Motility duration of SF was significantly higher (113.6 ± 22.6 s) than that of CS at 3 months (71 ± 12.2 s). The spermatic viability of SF was higher (97.1 ± 1%) in comparison with CS treatments. Sperm anomalies in SF were lower (12.4 ± 3.2%) than in all other treatments. ATP contents of (1.74 ± 4.5 nM × 10⁸ sptz) were superior only (<i>p</i> ≤ 0.05) in CS of 1 month (1.16 ± 1.2 nM × 10⁸ sptz). TAC was higher (<i>p</i> ≤ 0.05) in SF (26.46 ± 1.2 mM of Trolox [6-hydroxy-2,5,7,8-tetramethilcromane-acid 2-carboxylic]) compared to other CS treatments. However, there were not any significant differences in this parameter between cryopreservation times (<i>p</i> ≤ 0.05). Fertility rates were the same for all treatments. In terms of cellular ultrastructure, it was observed that <i>B. amazonicus</i> sptz had an oval-shaped head without an acrosome, middle part, and only one flagellum.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/6671651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Body Weight, Temperature, and Daily Time Periods on Oxygen Consumption Rate of Juvenile Silver Pomfret (Pampus argenteus)","authors":"Yu Liu,&nbsp;Minhai Liu,&nbsp;Jieyi Chen,&nbsp;Daning Jiang,&nbsp;Jingjie Hu,&nbsp;Zhihua Lin","doi":"10.1155/are/1513694","DOIUrl":"https://doi.org/10.1155/are/1513694","url":null,"abstract":"<div>\u0000 <p>Oxygen consumption rate (OCR), which reflects the metabolism requirement of fish, is regulated by body weight and temperature. However, the priority of body weight and temperature in determining the OCR of fish is not clear. The optimal temperature and feeding time for the commercially important silver pomfret (<i>Pampus argenteus</i>) in China were evaluated using two sizes of fish (15.8 ± 2.7 g, S; 35.3 ± 6.1 g, L) at four different water temperatures (21, 24, 27, 30°C) and four time periods (5:00–6:00, 11:00–12:00, 17:00–18:00, 23:00–24:00) in static respirometers. The results showed that the OCR of fish was significantly higher at 21 and 24°C than that at 27 and 30°C (<i>p</i> &lt; 0.05). The <i>Q</i>₁₀ increased from range of 21 and 24°C to range of 24–27°C but decreased from range of 24–27°C to range of 27–30°C. The body weight and water temperature significantly affected the OCR of fish (<i>p</i> &lt; 0.05). The OCR of S was significantly higher than that of L (<i>p</i> &lt; 0.05), while the OCR of fish increased linearly with increasing temperature. The OCR decreased with increasing fish body weight, and the coefficient (<i>a</i> – 1) in the linear regression equation of the logarithmic plot ranged from −0.568 to −0.191 (11.2–47.4 g, 21–30°C), with the mean value was −0.346. Overall, a two-factor regression equation for OCR of silver pomfret was established as OCR = 0.0558 × <i>W –</i>0.0081 × <i>T</i> + 0.0341 (<i>N</i> = 120, <i>R</i>² = 0.5626, <i>p</i> &lt; 0.05). The OCR was relatively higher at 5:00–6:00 compared to other time periods of the day. These results suggested that body weight exerted a more significant influence on the metabolic rate of silver pomfret compared to temperature. The optimal temperature for this fish species was between 27 and 30°C based on <i>Q</i>₁₀, and the recommended feeding time was at 5:00–6:00 for maximizing growth for silver pomfret.</p>\u0000 </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2025 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/1513694","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}