Animal Biotechnology最新文献

Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds. 不同牛品种CUBN与MIA3基因拷贝数变异及生长性状的关系

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-28 DOI: 10.1080/10495398.2025.2450355

Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu

{"title":"Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds.","authors":"Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu","doi":"10.1080/10495398.2025.2450355","DOIUrl":"10.1080/10495398.2025.2450355","url":null,"abstract":"Copy number variations (CNV) are important genetic variations. The endogenous factors cobalamin receptor (CUBN) and MIA SH3 domain ER-derived factor 3 (MIA3) are associated with bone/muscle development and intramuscular fat deposition. There have been no reports on the effects of CUBN and MIA3 CNVs on growth traits of Chinese cattle. This study aimed to determine the correlation between the CUBN and MIA3 CNVs and growth traits in Chinese cattle. qRT-PCR was used to detect the distribution of CUBN and MIA3 CNV and the expression levels of their mRNA, and correlation analysis was conducted between CNV and growth traits. The CUBN was differentially expressed in different breeds of cattle, and CUBN CNV correlated significantly with body height, hip height, body slanting length, and hip width of Grassland Red cattle (CYH); eye muscle area of Yanbian cattle (YB) and Yan Yellow cattle (YH). MIA3 showed no CNV in CYH and YB cattle, and only one deletion type occurred in YH cattle. CUBN and MIA3 mRNA have different expression patterns in different cattle breeds and tissues. In conclusion, CUBN CNV is correlated significantly with growth traits in Chinese cattle and is a novel molecular marker that could be exploited in cattle breeding.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450355"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep. INSL3和WNT2B基因多态性对新疆齐拉黑羊、哈萨克羊和多朗羊季节繁殖性状的影响

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-03 DOI: 10.1080/10495398.2025.2498677

Jingdong Bi, Sulaiman Yiming, Jiaqi Li, Quanfeng Wang, Manjun Zhai, Shaoqi Cao, Mengting Zhu, Hua Yang

{"title":"Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep.","authors":"Jingdong Bi, Sulaiman Yiming, Jiaqi Li, Quanfeng Wang, Manjun Zhai, Shaoqi Cao, Mengting Zhu, Hua Yang","doi":"10.1080/10495398.2025.2498677","DOIUrl":"https://doi.org/10.1080/10495398.2025.2498677","url":null,"abstract":"The purpose of this study was to investigate the polymorphism and genetic correlation of INSL3 and WNT2B genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of INSL3 and WNT2B genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in INSL3 gene and there were three genotypes in SNP2 (INSL3 (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the WNT2B gene and there were three genotypes in SNP4 (WNT2B (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (P > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (P < 0.05). Further, AT genotype of SNP2 (INSL3 (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The WNT2B significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. INSL3 (A100T) and WNT2B (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2498677"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome wide landscaping of copy number variations for horse inter-breed variability. 马种间变异拷贝数变异的全基因组景观分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-10 DOI: 10.1080/10495398.2024.2446251

Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai

{"title":"Genome wide landscaping of copy number variations for horse inter-breed variability.","authors":"Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai","doi":"10.1080/10495398.2024.2446251","DOIUrl":"https://doi.org/10.1080/10495398.2024.2446251","url":null,"abstract":"Copy number variations (CNVs) have become widely acknowledged as a significant source of genomic variability and phenotypic variance. To understand the genetic variants in horses, CNVs from six Indian horse breeds, namely, Manipuri, Zanskari, Bhutia, Spiti, Kathiawari and Marwari were discovered using Axiom™ Equine Genotyping Array. These breeds differed in agro-climatic adaptation with distinct phenotypic characters. A total of 2668 autosomal CNVs and 381 CNV regions (CNVRs) were identified with PennCNV tool. DeepCNV was employed to re-validate to get 883 autosomal CNVs, of which 9.06% were singleton type. A total of 180 CNVRs were identified after DeepCNV filtering with the estimated length of 3.12 Kb-4.90 Mb. The functional analysis showed the majority of the CNVRs genes enriched for sensory perception and olfactory receptor activity. An Equine CNVs database, EqCNVdb (http://backlin.cabgrid.res.in/eqcnvdb/) was developed which catalogues detailed information on the horse CNVs, CNVRs and gene content within CNVRs. Also, three random CNVRs were validated with real-time polymerase chain reaction. These findings will aid in the understanding the horse genome and serve as a preliminary foundation for future CNV association research with commercially significant equine traits. The identification of CNVs and CNVRs would lead to better insights into genetic basis of important traits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2446251"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced susceptibility of porcine muscle-derived mesenchymal stem cells to Aujeszky's virus compared Vero cells. 与Vero细胞相比，猪肌肉源性间充质干细胞对奥耶斯基病毒的易感性增强。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-20 DOI: 10.1080/10495398.2025.2479677

Na-Yeon Gu, Gwang Sik Ryu, Gyu-Nam Park, Ju-Yeon Lee, Yun Sang Cho, Dong-Kun Yang, Hye Jeong Lee

{"title":"Enhanced susceptibility of porcine muscle-derived mesenchymal stem cells to Aujeszky's virus compared Vero cells.","authors":"Na-Yeon Gu, Gwang Sik Ryu, Gyu-Nam Park, Ju-Yeon Lee, Yun Sang Cho, Dong-Kun Yang, Hye Jeong Lee","doi":"10.1080/10495398.2025.2479677","DOIUrl":"10.1080/10495398.2025.2479677","url":null,"abstract":"Mesenchymal stem cells (MSCs) can self-renew and differentiate into several lineages and can be isolated from different tissues such as bone marrow, adipose tissue, umbilical cord blood, and muscle. Herein, we established MSCs derived from miniature pig muscle (MpMu-MSCs) and assessed their response to Aujeszky's virus. We characterized the MpMu-MSCs based on their cellular morphology, proliferation properties, cell surface marker expression, and mesodermal differentiation potential. MpMu-MSCs demonstrated a fibroblast-like spindle shape and formed a homogeneous monolayer. They showed a considerable increase in cell proliferation over 16 passages. The cells expressed surface markers CD29, CD44, CD90, and CD105 and demonstrated mesodermal lineage differentiation capabilities. MpMu-MSCs demonstrated faster cytopathic effects than the Vero cells when infected with Aujeszky's virus. The virus titer in MpMu-MSCs was initiated at 101.4 TCID50/ml at 12 h post-infection (hpi) and increased to 106.6 TCID50/ml at 72 hpi. In Vero cells, it was initiated at 102.3 TCID50/ml at 48 hpi and increased to 103.8 TCID50/ml at 72 hpi. This study showed that the stem cells procured from miniature pig muscles exhibit MSC characteristics and that the established cells demonstrate higher susceptibility and virus titer to Aujeszky's virus than Vero cells, indicating their potential use in virus research.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2479677"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits. 肌生成抑制素缺乏家兔骨骼肌发育中circrna和lncrna参与的综合分析

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-26 DOI: 10.1080/10495398.2025.2465624

Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan

{"title":"Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits.","authors":"Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan","doi":"10.1080/10495398.2025.2465624","DOIUrl":"10.1080/10495398.2025.2465624","url":null,"abstract":"Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three WT and four MSTN KO rabbits, we analyzed the expression profiles of circRNAs and lncRNAs. After identifying the differently expressed RNAs, the biological pathways implicated were ascertained by performing enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). We identified differences in the expression of 251 circRNAs (79 upregulated and 172 downregulated), 176 lncRNAs (53 upregulated and 123 downregulated), and 1178 mRNAs (408 upregulated and 770 downregulated) between WT and MSTN KO rabbits. Target genes were significantly enriched in pathways associated with protein synthesis and catabolism, such as oxidative phosphorylation, ubiquitin-mediated proteolysis, the FoxO signaling pathway, and the pentose phosphate pathway, as identified through GO and KEGG enrichment analyses. The constructed network indicates that a class of circRNAs and lncRNAs is engaged in MSTN-mediated regulation of skeletal muscle development. These findings provide valuable insights for innovative therapeutic, diagnostic, and preventive approaches to muscle disorders.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2465624"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eucommia ulmoides and its inhibitory effects on prevotella in piglet gut microbiome through metagenomic and metabolomic analysis. 杜仲及其对仔猪肠道微生物群普氏菌的抑制作用。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/10495398.2025.2503753

Shanshan Han, Qiang Zhang, Hongyan Zhang, Jian Ma

{"title":"Eucommia ulmoides and its inhibitory effects on prevotella in piglet gut microbiome through metagenomic and metabolomic analysis.","authors":"Shanshan Han, Qiang Zhang, Hongyan Zhang, Jian Ma","doi":"10.1080/10495398.2025.2503753","DOIUrl":"https://doi.org/10.1080/10495398.2025.2503753","url":null,"abstract":"Eucommia ulmoides (EU) is a traditional medicinal plant widely cultivated across China. The combination of EU and feed significantly affects the growth performance, intestinal microbiota composition, and metabolic characteristics of weaned piglets. Forty Landrace x Yorkshire piglets were randomly assigned to four groups: a control group receiving a basal diet, three treatment groups receiving a basal diet supplemented with EU and EU with mix energy (EU+ME), and EU with high protein and energy (EU+HPE), respectively. Growth performance was monitored over a 25-day feeding period, and fecal samples were collected for subsequent metagenomic sequencing and metabolomic analysis. Piglets supplemented with EU, EU+ME, and EU+HPE exhibited significantly improved growth performance, compared to the control group. Metagenomic analysis revealed significant alterations in gut microbiota composition, with increased beneficial bacterial classes and suppression of Prevotella spp. Metabolomic profiling demonstrated distinct metabolic alterations among the treatment groups, with pathway impact analysis highlighting enhanced protein synthesis and energy metabolism. Furthermore, EU supplementation did not affect porcine epidemic diarrhea virus activity in vitro but reduced LPS-induced intestinal inflammation. These findings suggest that EU could be a promising natural additive for improving piglet health and growth, with potential implications for managing post-weaning challenges in swine production.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503753"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization and insights into the belted coat pattern of a local, reconstituted pig population. 基因组表征和见解到一个地方，重组猪群的带状被毛模式。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/10495398.2025.2515462

Samira Giovannini, Maria Giuseppina Strillacci, Raffaella Milanesi, Caterina Altissimi, Massimo Biagetti, Francesca Maria Sarti

{"title":"Genomic characterization and insights into the belted coat pattern of a local, reconstituted pig population.","authors":"Samira Giovannini, Maria Giuseppina Strillacci, Raffaella Milanesi, Caterina Altissimi, Massimo Biagetti, Francesca Maria Sarti","doi":"10.1080/10495398.2025.2515462","DOIUrl":"https://doi.org/10.1080/10495398.2025.2515462","url":null,"abstract":"The Cinghiato pig population originates from a breeding project aimed at reconstituting an extinct local swine breed, historically depicted in frescoes in Umbria, Central Italy. The selection strategy employed a reconstruction breeding program, choosing mating pairs based on the unique coat phenotype represented in these artworks. Unlike the traditional Cinta Senese breed, Cinghiato pigs exhibit a white belt encircling the trunk, while their forelimbs remain black. This study explores the genetic background of the belted coat pattern observed in the heterogeneous reconstituted population. Twenty-two pigs were genotyped using the Porcine GGP 80K SNP BeadChip. Genetic analyses were conducted to assess population structure and diversity, with comparisons made to other Italian pig breeds and wild boars. Findings reveal moderate genetic diversity within the Cinghiato population. Runs of Homozygosity patterns suggest historical inbreeding events. Moreover, several genomic regions were associated with traits relevant to niche pork production, including feed intake, leg conformation, and fat deposition. Polymorphisms were detected in 10 coat color-related genes (KIT, MC1R, ASIP, EDNRB, KITLG, MITF, OCA2, PAX3, SOX10, and TYRP1). Although some candidate variants were identified, this preliminary study highlights the need for further research to clarify the genetic mechanisms underlying the phenotypic variability of belted coat patterns.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2515462"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL14 and WTAP play a crucial role in the regulation of bovine preadipocyte differentiation. METTL14和WTAP在牛前脂肪细胞的分化调控中起重要作用。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2476531

Jia Liu, Chicheng Ma, Yu Cheng, Minzhi Wang, Guoqing Zhao, Liwei Huang, Ruigao Song, Xi Wang, Hongxia Li

引用次数: 0

Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing. 基于全基因组重测序的莆田黑鸭蛋重性状候选基因鉴定

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-17 DOI: 10.1080/10495398.2025.2503754

Yinhua Yang, Weilong Lin, Huihuang Li, Fan Yang, Xinguo Bao, Chengfu Pan, Lianjie Lai, Weimin Lin, Ruiyi Lin

{"title":"Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing.","authors":"Yinhua Yang, Weilong Lin, Huihuang Li, Fan Yang, Xinguo Bao, Chengfu Pan, Lianjie Lai, Weimin Lin, Ruiyi Lin","doi":"10.1080/10495398.2025.2503754","DOIUrl":"https://doi.org/10.1080/10495398.2025.2503754","url":null,"abstract":"Egg weight is a primary economic trait in poultry breeding. Putian Black duck, an excellent local laying duck breed in Fujian Province, includes two different strains, black feather strain and white feather strain. The white feather strain of Putian Black duck is also known as Putian White duck. Except for the different feather colors, these two strains differ in egg weight. In this study, whole-genome resequencing was conducted on Putian Black duck and Putian White duck to explore the differences in the genetic mechanism of egg weight. LRP8, VLDLR, and LPL were identified as key candidate genes affecting egg weight. Mass spectrometry was used to detect the SNPs of LRP8, VLDLR, and LPL. Result indicates that the SNPs of LRP8, VLDLR, and LPL in both populations exhibited moderate polymorphism, and Putian Black duck possessed higher genetic variation and potential selectivity. Association analysis indicated that in Putian Black duck, four SNPs in the LRP8 gene were significantly associated with egg weight. These loci can be used as molecular markers for improving egg weight in Putian Black duck.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503754"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat. 南江黄山羊IGF2BP1第二内含子snp的鉴定及其与生长性状的关系

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/10495398.2025.2461176

Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao

{"title":"Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat.","authors":"Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao","doi":"10.1080/10495398.2025.2461176","DOIUrl":"10.1080/10495398.2025.2461176","url":null,"abstract":"Insulin-like Growth Factor 2 mRNA-binding Protein 1 (IGF2BP1) is a candidate gene of significant interest for modulating economically important traits in livestock and poultry. The second intron of IGF2BP1 has been implicated in growth-related traits, though its precise mechanistic role remains elusive. Initial resequencing analyses in our laboratory indicated strong selective pressures on the IGF2BP1 genomic region, prompting the selection and identification of several single nucleotide polymorphisms (SNPs). Seven SNPs were mapped to the conserved region of the second intron, necessitating further investigation into their functional relevance and association with growth traits. In this study, 348 Nanjiang Yellow goats were analyzed, and the association analysis via the GLM program in SAS 9.4 identified five SNPs significantly correlated with growth traits. Notably, rs652062749(A > G) emerged as a critical locus influencing later-stage growth traits. Furthermore, strong linkage disequilibrium was observed among three SNPs, with the rs638185407 (T > A) variant markedly enhancing luciferase activity in H293T cells. Combination genotypes TTAACT, TTCCCC, and ATCACT were identified as superior for growth traits, offering theoretical insights for genetic co-breeding. This study underscores the potential utility of IGF2BP1 as a functional genetic marker in Nanjiang Yellow goat breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2461176"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0