Animal Biotechnology最新文献

Genome wide landscaping of copy number variations for horse inter-breed variability. 马种间变异拷贝数变异的全基因组景观分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-10 DOI: 10.1080/10495398.2024.2446251

Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai

{"title":"Genome wide landscaping of copy number variations for horse inter-breed variability.","authors":"Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai","doi":"10.1080/10495398.2024.2446251","DOIUrl":"https://doi.org/10.1080/10495398.2024.2446251","url":null,"abstract":"Copy number variations (CNVs) have become widely acknowledged as a significant source of genomic variability and phenotypic variance. To understand the genetic variants in horses, CNVs from six Indian horse breeds, namely, Manipuri, Zanskari, Bhutia, Spiti, Kathiawari and Marwari were discovered using Axiom™ Equine Genotyping Array. These breeds differed in agro-climatic adaptation with distinct phenotypic characters. A total of 2668 autosomal CNVs and 381 CNV regions (CNVRs) were identified with PennCNV tool. DeepCNV was employed to re-validate to get 883 autosomal CNVs, of which 9.06% were singleton type. A total of 180 CNVRs were identified after DeepCNV filtering with the estimated length of 3.12 Kb-4.90 Mb. The functional analysis showed the majority of the CNVRs genes enriched for sensory perception and olfactory receptor activity. An Equine CNVs database, EqCNVdb (http://backlin.cabgrid.res.in/eqcnvdb/) was developed which catalogues detailed information on the horse CNVs, CNVRs and gene content within CNVRs. Also, three random CNVRs were validated with real-time polymerase chain reaction. These findings will aid in the understanding the horse genome and serve as a preliminary foundation for future CNV association research with commercially significant equine traits. The identification of CNVs and CNVRs would lead to better insights into genetic basis of important traits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2446251"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-28 DOI: 10.1080/10495398.2025.2450355

Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu

{"title":"Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds.","authors":"Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu","doi":"10.1080/10495398.2025.2450355","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450355","url":null,"abstract":"Copy number variations (CNV) are important genetic variations. The endogenous factors cobalamin receptor (CUBN) and MIA SH3 domain ER-derived factor 3 (MIA3) are associated with bone/muscle development and intramuscular fat deposition. There have been no reports on the effects of CUBN and MIA3 CNVs on growth traits of Chinese cattle. This study aimed to determine the correlation between the CUBN and MIA3 CNVs and growth traits in Chinese cattle. qRT-PCR was used to detect the distribution of CUBN and MIA3 CNV and the expression levels of their mRNA, and correlation analysis was conducted between CNV and growth traits. The CUBN was differentially expressed in different breeds of cattle, and CUBN CNV correlated significantly with body height, hip height, body slanting length, and hip width of Grassland Red cattle (CYH); eye muscle area of Yanbian cattle (YB) and Yan Yellow cattle (YH). MIA3 showed no CNV in CYH and YB cattle, and only one deletion type occurred in YH cattle. CUBN and MIA3 mRNA have different expression patterns in different cattle breeds and tissues. In conclusion, CUBN CNV is correlated significantly with growth traits in Chinese cattle and is a novel molecular marker that could be exploited in cattle breeding.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450355"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-26 DOI: 10.1080/10495398.2025.2465624

Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan

{"title":"Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits.","authors":"Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan","doi":"10.1080/10495398.2025.2465624","DOIUrl":"https://doi.org/10.1080/10495398.2025.2465624","url":null,"abstract":"Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three WT and four MSTN KO rabbits, we analyzed the expression profiles of circRNAs and lncRNAs. After identifying the differently expressed RNAs, the biological pathways implicated were ascertained by performing enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). We identified differences in the expression of 251 circRNAs (79 upregulated and 172 downregulated), 176 lncRNAs (53 upregulated and 123 downregulated), and 1178 mRNAs (408 upregulated and 770 downregulated) between WT and MSTN KO rabbits. Target genes were significantly enriched in pathways associated with protein synthesis and catabolism, such as oxidative phosphorylation, ubiquitin-mediated proteolysis, the FoxO signaling pathway, and the pentose phosphate pathway, as identified through GO and KEGG enrichment analyses. The constructed network indicates that a class of circRNAs and lncRNAs is engaged in MSTN-mediated regulation of skeletal muscle development. These findings provide valuable insights for innovative therapeutic, diagnostic, and preventive approaches to muscle disorders.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2465624"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced susceptibility of porcine muscle-derived mesenchymal stem cells to Aujeszky's virus compared Vero cells.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-20 DOI: 10.1080/10495398.2025.2479677

Na-Yeon Gu, Gwang Sik Ryu, Gyu-Nam Park, Ju-Yeon Lee, Yun Sang Cho, Dong-Kun Yang, Hye Jeong Lee

{"title":"Enhanced susceptibility of porcine muscle-derived mesenchymal stem cells to Aujeszky's virus compared Vero cells.","authors":"Na-Yeon Gu, Gwang Sik Ryu, Gyu-Nam Park, Ju-Yeon Lee, Yun Sang Cho, Dong-Kun Yang, Hye Jeong Lee","doi":"10.1080/10495398.2025.2479677","DOIUrl":"https://doi.org/10.1080/10495398.2025.2479677","url":null,"abstract":"Mesenchymal stem cells (MSCs) can self-renew and differentiate into several lineages and can be isolated from different tissues such as bone marrow, adipose tissue, umbilical cord blood, and muscle. Herein, we established MSCs derived from miniature pig muscle (MpMu-MSCs) and assessed their response to Aujeszky's virus. We characterized the MpMu-MSCs based on their cellular morphology, proliferation properties, cell surface marker expression, and mesodermal differentiation potential. MpMu-MSCs demonstrated a fibroblast-like spindle shape and formed a homogeneous monolayer. They showed a considerable increase in cell proliferation over 16 passages. The cells expressed surface markers CD29, CD44, CD90, and CD105 and demonstrated mesodermal lineage differentiation capabilities. MpMu-MSCs demonstrated faster cytopathic effects than the Vero cells when infected with Aujeszky's virus. The virus titer in MpMu-MSCs was initiated at 101.4 TCID50/ml at 12 h post-infection (hpi) and increased to 106.6 TCID50/ml at 72 hpi. In Vero cells, it was initiated at 102.3 TCID50/ml at 48 hpi and increased to 103.8 TCID50/ml at 72 hpi. This study showed that the stem cells procured from miniature pig muscles exhibit MSC characteristics and that the established cells demonstrate higher susceptibility and virus titer to Aujeszky's virus than Vero cells, indicating their potential use in virus research.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2479677"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL14 and WTAP play a crucial role in the regulation of bovine preadipocyte differentiation.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2476531

Jia Liu, Chicheng Ma, Yu Cheng, Minzhi Wang, Guoqing Zhao, Liwei Huang, Ruigao Song, Xi Wang, Hongxia Li

引用次数: 0

Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-24 DOI: 10.1080/10495398.2025.2450364

Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

{"title":"Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.","authors":"Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2450364","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450364","url":null,"abstract":"Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets. Additionally, Madakhlasht sheep, which sometimes produce twins, and Balkhi sheep, which produce only singletons, were used as references. The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. Notably, two point mutations and a six-nucleotide insertion were found upstream of the initiation codon in twin-producing Kari ewes, potentially affecting ZP3 expression and LS. Two missense mutations (I101L in exon 1 and R408H in exon 8) were heterozygous in twin-producing Kari ewes but homozygous in other groups, correlating with LS. Protein modelling suggested that the I101L mutation alters the binding site, potentially impacting protein function. These findings indicate that ZP3 gene variations influence reproductive efficiency and LS in sheep, with specific variants serving as potential markers for selective breeding to enhance LS.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450364"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on immortalization of Mongolian sheep fibroblast cells.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-07 DOI: 10.1080/10495398.2025.2459915

Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao

{"title":"Study on immortalization of Mongolian sheep fibroblast cells.","authors":"Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao","doi":"10.1080/10495398.2025.2459915","DOIUrl":"https://doi.org/10.1080/10495398.2025.2459915","url":null,"abstract":"This study aims to establish an immortalized fibroblast cell line from Mongolian sheep. Primary Mongolian sheep fibroblasts (SSF) were isolated using tissue explant and enzymatic digestion methods, followed by microscopic observation, growth curve plotting, and karyotype analysis. The results confirmed the successful isolation of SSF. Human (hTERT) and sheep (sTERT) telomerase reverse transcriptase vectors were separately introduced into SSF, with cells passaged up to 36 generations following G418 selection. Microscopic examination and qRT-PCR results demonstrated that TERT transfection did not alter the morphology of SSF and led to stable, high levels of TERT expression (P < 0.01). Cell counting and flow cytometry revealed that TERT-transfected cells had higher viability and lower apoptosis rates compared to SSF (P < 0.05). Karyotype and soft agar colony formation assays indicated that hTERT and sTERT-transfected cells maintained normal characteristics without malignant transformation. β-galactosidase staining indicated that TERT transfection significantly reduced cellular senescence (P < 0.001). Additionally, sTERT-transfected cells exhibited higher TERT expression, enhanced viability, proliferation, and anti-senescence effects compared to hTERT-transfected cells (P < 0.05). In summary, the introduction of hTERT and sTERT effectively extends the lifespan of SSF, with sTERT demonstrating a more pronounced effect. This study provides critical evidence for preserving Mongolian sheep genetic resources and developing immortalized cell lines.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2459915"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-17 DOI: 10.1080/10495398.2025.2461176

Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao

{"title":"Identification of SNPs in the second intron of IGF2BP1 and their Association with growth traits in Nanjiang Yellow goat.","authors":"Shuheng Chen, Liang Xu, Junchen Leng, Zitong Chen, Yu Chen, Li Li, Hongping Zhang, Mingzhou Li, Jiaxue Cao","doi":"10.1080/10495398.2025.2461176","DOIUrl":"https://doi.org/10.1080/10495398.2025.2461176","url":null,"abstract":"Insulin-like Growth Factor 2 mRNA-binding Protein 1 (IGF2BP1) is a candidate gene of significant interest for modulating economically important traits in livestock and poultry. The second intron of IGF2BP1 has been implicated in growth-related traits, though its precise mechanistic role remains elusive. Initial resequencing analyses in our laboratory indicated strong selective pressures on the IGF2BP1 genomic region, prompting the selection and identification of several single nucleotide polymorphisms (SNPs). Seven SNPs were mapped to the conserved region of the second intron, necessitating further investigation into their functional relevance and association with growth traits. In this study, 348 Nanjiang Yellow goats were analyzed, and the association analysis via the GLM program in SAS 9.4 identified five SNPs significantly correlated with growth traits. Notably, rs652062749(A > G) emerged as a critical locus influencing later-stage growth traits. Furthermore, strong linkage disequilibrium was observed among three SNPs, with the rs638185407 (T > A) variant markedly enhancing luciferase activity in H293T cells. Combination genotypes TTAACT, TTCCCC, and ATCACT were identified as superior for growth traits, offering theoretical insights for genetic co-breeding. This study underscores the potential utility of IGF2BP1 as a functional genetic marker in Nanjiang Yellow goat breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2461176"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2471785

Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao

{"title":"The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.","authors":"Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao","doi":"10.1080/10495398.2025.2471785","DOIUrl":"https://doi.org/10.1080/10495398.2025.2471785","url":null,"abstract":"In order to study the distribution characteristics of intestinal microbiota and antibiotic resistance genes (ARGs) in Duzang pigs after adding stems and leaves of Panax notoginseng to the feed, the characteristics of intestinal microbiota were explored by metagenomic sequencing, and 14 ARGs and 2 integrase genes were detected by qPCR. The results showed that the addition of stems and leaves of P. notoginseng increased the relative abundance of Firmicutes, Lactobacillus and Pediococcus in the cecum of Duzang pigs. A total of 10 ARGs and 2 integrase genes were detected in the cecal contents of pigs. The addition of stems and leaves of P. notoginseng reduced the relative abundance of total ARGs, ermB, tetO and tetW in the cecum of Duzang pigs. The results of network analysis showed that multiple genera were potential hosts of ARGs. The addition of stems and leaves of P. notoginseng may reduce the relative abundance of ARGs by reducing the relative abundance of genera such as Corynebacterium and Flavonifractor, thereby reducing the risk of ARGs spread. This study provides a theoretical basis for the rational use of stems and leaves of P. notoginseng to control ARGs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2471785"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats. 阴阳补充对奶山羊繁殖性能、抗氧化性和免疫力的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/10495398.2025.2450349

Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai

{"title":"Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats.","authors":"Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai","doi":"10.1080/10495398.2025.2450349","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450349","url":null,"abstract":"This study aims to explore the effects of Yin and Yang Double Supplement Compound Chinese Medicine Preparations (YYSBFF) on the reproductive performance, antioxidant levels, and immunity of dairy goats. For the experiment, 36 Alps milk goats were selected and randomly divided into an experimental group and a control group, with 18 goats in each group. The ewes in the experimental group were fed with YYSBFF for 14 d prior to breeding and farrowing. The results of the experiment showed that the estrus rate, embryo acceptance rate, and lamb birth weight in the experimental group were higher than those in the control group, and the weak lamb rate was significantly lower. Moreover, the experimental group exhibited higher levels of reproductive hormones (FSH, LH), antioxidant factors (T-SOD, GSH-Px, MDA), and immunoglobulins (IgA, IgM, IgG) compared to the control group. There were no significant differences in liver and kidney function indicators (ALT, AST, TP, ALB, CREA, UREA) between the experimental and control groups (p > .05). These findings indicate that YYSBFF can enhance the reproductive performance of dairy goats by regulating the level of sex hormones, while also improving the body's antioxidant and immune abilities.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450349"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0