Animal Biotechnology最新文献

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Genome wide landscaping of copy number variations for horse inter-breed variability. 马种间变异拷贝数变异的全基因组景观分析。
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-10 DOI: 10.1080/10495398.2024.2446251
Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai
{"title":"Genome wide landscaping of copy number variations for horse inter-breed variability.","authors":"Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T K Bhattacharya, Dinesh Kumar, Anil Rai","doi":"10.1080/10495398.2024.2446251","DOIUrl":"https://doi.org/10.1080/10495398.2024.2446251","url":null,"abstract":"<p><p>Copy number variations (CNVs) have become widely acknowledged as a significant source of genomic variability and phenotypic variance. To understand the genetic variants in horses, CNVs from six Indian horse breeds, <i>namely,</i> Manipuri, Zanskari, Bhutia, Spiti, Kathiawari and Marwari were discovered using Axiom<sup>™</sup> Equine Genotyping Array. These breeds differed in agro-climatic adaptation with distinct phenotypic characters. A total of 2668 autosomal CNVs and 381 CNV regions (CNVRs) were identified with PennCNV tool. DeepCNV was employed to re-validate to get 883 autosomal CNVs, of which 9.06% were singleton type. A total of 180 CNVRs were identified after DeepCNV filtering with the estimated length of 3.12 Kb-4.90 Mb. The functional analysis showed the majority of the CNVRs genes enriched for sensory perception and olfactory receptor activity. An Equine CNVs database, EqCNVdb (http://backlin.cabgrid.res.in/eqcnvdb/) was developed which catalogues detailed information on the horse CNVs, CNVRs and gene content within CNVRs. Also, three random CNVRs were validated with real-time polymerase chain reaction. These findings will aid in the understanding the horse genome and serve as a preliminary foundation for future CNV association research with commercially significant equine traits. The identification of CNVs and CNVRs would lead to better insights into genetic basis of important traits.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2446251"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds.
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-28 DOI: 10.1080/10495398.2025.2450355
Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu
{"title":"Relationship between the <i>CUBN</i> and the <i>MIA3</i> gene copy number variation and growth traits in different cattle breeds.","authors":"Yue Han, Jiwei Liu, Congcong Zhang, Ming Sun, Xuanyu Li, Hongliang Liu, Shengnan Li, Yongchao Zhu, Ruidong Li, Xiaotong Luo, Yumin Zhao, Jian Wu","doi":"10.1080/10495398.2025.2450355","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450355","url":null,"abstract":"<p><p>Copy number variations (CNV) are important genetic variations. The endogenous factors cobalamin receptor (<i>CUBN</i>) and MIA SH3 domain ER-derived factor 3 (<i>MIA3</i>) are associated with bone/muscle development and intramuscular fat deposition. There have been no reports on the effects of <i>CUBN</i> and <i>MIA3</i> CNVs on growth traits of Chinese cattle. This study aimed to determine the correlation between the <i>CUBN</i> and <i>MIA3</i> CNVs and growth traits in Chinese cattle. qRT-PCR was used to detect the distribution of <i>CUBN</i> and <i>MIA3</i> CNV and the expression levels of their mRNA, and correlation analysis was conducted between CNV and growth traits. The <i>CUBN</i> was differentially expressed in different breeds of cattle, and <i>CUBN</i> CNV correlated significantly with body height, hip height, body slanting length, and hip width of Grassland Red cattle (CYH); eye muscle area of Yanbian cattle (YB) and Yan Yellow cattle (YH). <i>MIA3</i> showed no CNV in CYH and YB cattle, and only one deletion type occurred in YH cattle. <i>CUBN</i> and <i>MIA3</i> mRNA have different expression patterns in different cattle breeds and tissues. In conclusion, <i>CUBN</i> CNV is correlated significantly with growth traits in Chinese cattle and is a novel molecular marker that could be exploited in cattle breeding.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450355"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-24 DOI: 10.1080/10495398.2025.2450364
Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni
{"title":"Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.","authors":"Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2450364","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450364","url":null,"abstract":"<p><p>Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets. Additionally, Madakhlasht sheep, which sometimes produce twins, and Balkhi sheep, which produce only singletons, were used as references. The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. Notably, two point mutations and a six-nucleotide insertion were found upstream of the initiation codon in twin-producing Kari ewes, potentially affecting ZP3 expression and LS. Two missense mutations (I101L in exon 1 and R408H in exon 8) were heterozygous in twin-producing Kari ewes but homozygous in other groups, correlating with LS. Protein modelling suggested that the I101L mutation alters the binding site, potentially impacting protein function. These findings indicate that ZP3 gene variations influence reproductive efficiency and LS in sheep, with specific variants serving as potential markers for selective breeding to enhance LS.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450364"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats. 阴阳补充对奶山羊繁殖性能、抗氧化性和免疫力的影响。
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/10495398.2025.2450349
Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai
{"title":"Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats.","authors":"Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai","doi":"10.1080/10495398.2025.2450349","DOIUrl":"https://doi.org/10.1080/10495398.2025.2450349","url":null,"abstract":"<p><p>This study aims to explore the effects of Yin and Yang Double Supplement Compound Chinese Medicine Preparations (YYSBFF) on the reproductive performance, antioxidant levels, and immunity of dairy goats. For the experiment, 36 Alps milk goats were selected and randomly divided into an experimental group and a control group, with 18 goats in each group. The ewes in the experimental group were fed with YYSBFF for 14 d prior to breeding and farrowing. The results of the experiment showed that the estrus rate, embryo acceptance rate, and lamb birth weight in the experimental group were higher than those in the control group, and the weak lamb rate was significantly lower. Moreover, the experimental group exhibited higher levels of reproductive hormones (FSH, LH), antioxidant factors (T-SOD, GSH-Px, MDA), and immunoglobulins (IgA, IgM, IgG) compared to the control group. There were no significant differences in liver and kidney function indicators (ALT, AST, TP, ALB, CREA, UREA) between the experimental and control groups (<i>p</i> > .05). These findings indicate that YYSBFF can enhance the reproductive performance of dairy goats by regulating the level of sex hormones, while also improving the body's antioxidant and immune abilities.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450349"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation. 槲皮素通过调节肝脏脂质代谢、氧化应激和炎症来缓解代谢相关的脂肪肝疾病。
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2024-12-24 DOI: 10.1080/10495398.2024.2442351
Ling Jiang, Rong Yi, Huan Chen, Shuwu Wu
{"title":"Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation.","authors":"Ling Jiang, Rong Yi, Huan Chen, Shuwu Wu","doi":"10.1080/10495398.2024.2442351","DOIUrl":"https://doi.org/10.1080/10495398.2024.2442351","url":null,"abstract":"<p><p>The natural flavonoid quercetin, which exhibits a range of biological activities, has been implicated in liver disease resistance in recent research. <i>In vivo</i> study attesting to quercetin's protective effect against metabolic-associated fatty liver disease (MAFLD) is inadequate, however. Here, our investigation explored the potential benefits of quercetin in preventing MAFLD in C57BL/6 mice fed a high-fat diet (HFD). The results revealed that quercetin ameliorated the aberrant enhancement of body and liver weight. The hepatic histological anomalie induced by MAFLD were also mitigated by quercetin. HFD-induced imbalance in serum LDL, HDL, AST, ALT, TG, and LDH was mitigated by quercetin. Mechanically, we found that quercetin improved lipid metabolism by reducing lipogenesis proteins including ACC, FASN, and SREBP-1c and enhancing β-oxidation proteins including PPARα and CPT1A. <i>In vitro</i> study demonstrated that quercetin regulated hepatic lipid metabolism by targeting SREBP-1c and PPARα. Additionally, quercetin enhanced the antioxidant capacity in HFD-treated mice by downregulating Nrf2 and HO-1 expressions and upregulating SOD and GPX1 expressions. The hyper-activation of inflammation was also restored by quercetin via eliminating the phosphorylation of IκBα and NF-κB p65. Collectively, our observations highlight that quercetin exerts hepatoprotective properties in MAFLD mice by regulating hepatic lipid metabolism, oxidative stress and inflammatory response.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2442351"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exposure to Imidacloprid under variable conditions disturbs the muscle fatty acid profile of a fresh water non target fish: Labeo rohita. 在不同条件下接触吡虫啉会扰乱淡水非目标鱼类的肌肉脂肪酸谱:Labeo rohita。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-23 DOI: 10.1080/10495398.2024.2307020
Shazia Qadir, Muhammad Latif, Wen-Feng Wu, Fengqin Feng, Wadi B Alonazi, Arwah Amjad, Chien-Chin Chen, Zia Ur Rehman, Adil Khan, Furhan Iqbal
{"title":"Exposure to Imidacloprid under variable conditions disturbs the muscle fatty acid profile of a fresh water non target fish: <i>Labeo rohita</i>.","authors":"Shazia Qadir, Muhammad Latif, Wen-Feng Wu, Fengqin Feng, Wadi B Alonazi, Arwah Amjad, Chien-Chin Chen, Zia Ur Rehman, Adil Khan, Furhan Iqbal","doi":"10.1080/10495398.2024.2307020","DOIUrl":"10.1080/10495398.2024.2307020","url":null,"abstract":"<p><p>Economy of Pakistan is heavily dependent upon agriculture and extensive use of pesticide is quiet common to enhance the crop yield. Imidacloprid is among the first choice pesticides in Pakistan and it has been reported that through run off along with water it ends up in water bodies affecting non target aquatic fauna. Through the present investigation, we are reporting the effects of Imidacloprid on the fatty acids composition of a non-target, commercially important carp: <i>Labeo rohita</i>. Fish were exposed to sub lethal concentration of Imidacloprid (120 mgL<sup>1</sup>) for 2, 4 and 8 days (short term) as well as for 16, 32 and 64 days (long term experimental conditions). Pesticide untreated controls were also maintained for each treatment. Following the specific Imidacloprid exposure, fatty acid composition (%) was determined in the muscle of all experimental groups by using gas chromatography. Fish exposed to Imidacloprid for 8 days had reduced Palmitic acid (<i>p</i> = 0.02) and elevated muscle Arachidic acid (<i>p</i> < 0.001) than control group. <i>Labeo rohita</i> exposed to the pesticide for 32 days had elevated muscle Oleic (<i>p</i> = 0.02) and Linoleic acid (<i>p</i> = 0.02) while fish exposed to Imidacloprid to 64 days had reduced muscle Palmitic (<i>p</i> = 0.04) and Oleic acid (<i>p</i> = 0.03). In conclusion, we are reporting that the exposure to sub lethal concentration of Imidacloprid disturb the muscle fatty acid composition of <i>Labeo rohita</i> that may affect its food quality. The effects were more pronounced under long term experimental conditions and were probably due to potentiating lipid peroxidation and disturbed fish metabolism upon Imidacloprid exposure.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2307020"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139519254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity assessment of a lesser known buffalo population from Central India and its comparative evaluation reveals presence of sufficient genetic variation and absence of selection. 对印度中部一个鲜为人知的水牛种群进行的多样性评估及其比较评价显示,该种群存在足够的遗传变异,而且没有经过选择。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-25 DOI: 10.1080/10495398.2024.2305550
Rajesh Kumar Gahlyan, Vikas Vohra, Supriya Chhotaray, R S Kataria
{"title":"Diversity assessment of a lesser known buffalo population from Central India and its comparative evaluation reveals presence of sufficient genetic variation and absence of selection.","authors":"Rajesh Kumar Gahlyan, Vikas Vohra, Supriya Chhotaray, R S Kataria","doi":"10.1080/10495398.2024.2305550","DOIUrl":"10.1080/10495398.2024.2305550","url":null,"abstract":"<p><p>Planned breeding and conservation strategies for a lesser-known population require an assessment of complete genetic diversity and population structure analysis in addition to its morphometric characteristics. In the present study, a comparative analysis of the genetic structure of a rare buffalo population, namely Chhattisgarhi, was extensively studied using a panel of FAO-recommended microsatellite markers along with well-established breeds namely Murrah, Nili-Ravi, Gojri, Kalahandi, and Nagpuri. Mode shift analysis indicated the absence of genetic bottleneck in the recent past. Assessment of genetic diversity indices across all loci indicated the presence of sufficient genetic variation within and between populations. Analysis of molecular variance between the six different buffalo populations attributed 19.05% of the variations to between-population differentiation. Cluster analyses using DAPC and Bayesian approach along with the phylogenetic tree based on UPGMA grouped six populations into three groups. The Chhattisgarhi population was revealed to be genetically closer to Nagpuri and Kalahandi populations. The study reveals the presence of sufficient genetic diversity within the Chhattisgarhi population and indicates the absence of a systematic selection program. We suggest improvement and conservation programs should be planned for this breed in the near future through short-term selection.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2305550"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139545339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens. 水溶液微生物溶菌酶对肉鸡的益生菌后作用、抗炎作用和免疫调节作用。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-02-07 DOI: 10.1080/10495398.2024.2309955
Mustafa Bastamy, Ismail Raheel, Ahmed Elbestawy, Mohamed Diab, Enas Hammad, Lamiaa Elebeedy, Amal M El-Barbary, Ghadeer M Albadrani, Mohamed M Abdel-Daim, Mervat A Abdel-Latif, Ahmed Orabi
{"title":"Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens.","authors":"Mustafa Bastamy, Ismail Raheel, Ahmed Elbestawy, Mohamed Diab, Enas Hammad, Lamiaa Elebeedy, Amal M El-Barbary, Ghadeer M Albadrani, Mohamed M Abdel-Daim, Mervat A Abdel-Latif, Ahmed Orabi","doi":"10.1080/10495398.2024.2309955","DOIUrl":"10.1080/10495398.2024.2309955","url":null,"abstract":"<p><p>Lysozymes, efficient alternative supplements to antibiotics, have several benefits in poultry production. In the present study, 120, one-day-old, Ross 308 broiler chickens of mixed sex, were allocated into 2 equal groups, lysozyme treated group (LTG) and lysozyme free group (LFG), to evaluate the efficacy of lysozyme (Lysonir<sup>®</sup>) usage via both drinking water (thrice) and spray (once). LTG had better (<i>p</i> = 0.042) FCR, and higher European production efficiency factor compared to LFG (<i>p</i> = 0.042). The intestinal integrity score of LTG was decreased (<i>p</i> = 0.242) compared to that of LFG; 0.2 vs. 0.7. Higher (<i>p</i> ≤ 0.001) intestinal Lactobacillus counts were detected in chickens of LTG. Decreased (<i>p</i> ≤ 0.001) IL-1β and CXCL8 values were reported in LTG. The cellular immune modulation showed higher (<i>p</i> ≤ 0.001) opsonic activity (MΦ and phagocytic index) in LTG vs. LFG at 25 and 35 days. Also, higher (<i>p</i> ≤ 0.001) local, IgA, and humoral, HI titers, for both Newcastle, and avian influenza H5 viruses were found in LTG compared to LFG. In conclusion, microbial lysozyme could improve feed efficiency, intestinal integrity, <i>Lactobacillus</i> counts, anti-inflammatory, and immune responses in broiler chickens.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2309955"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139696820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Whole genome resequencing revealed genomic variants and functional pathways related to adaptation in Indian yak populations. 全基因组重测序揭示了与印度牦牛种群适应相关的基因组变异和功能途径。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-11-25 DOI: 10.1080/10495398.2023.2282723
Amod Kumar, Mahesh Dige, Saket Kumar Niranjan, Sonika Ahlawat, Reena Arora, Aneet Kour, Ramesh Kumar Vijh
{"title":"Whole genome resequencing revealed genomic variants and functional pathways related to adaptation in Indian yak populations.","authors":"Amod Kumar, Mahesh Dige, Saket Kumar Niranjan, Sonika Ahlawat, Reena Arora, Aneet Kour, Ramesh Kumar Vijh","doi":"10.1080/10495398.2023.2282723","DOIUrl":"10.1080/10495398.2023.2282723","url":null,"abstract":"<p><p>The present study aims to identify genomic variants through a whole genome sequencing (WGS) approach and uncover biological pathways associated with adaptation and fitness in Indian yak populations. A total of 30 samples (10 from each population) were included from Arunachali, Himachali and Ladakhi yak populations. WGS analysis revealed a total of 32171644, 27260825, and 32632460 SNPs and 4865254, 4429941, and 4847513 Indels in the Arunachali, Himachali, and Ladakhi yaks, respectively. Genes such as <i>RYR2</i>, <i>SYNE2</i>, <i>BOLA</i>, <i>HF1</i>, and the novel transcript <i>ENSBGRG00000011079</i> were found to have the maximum number of high impact variants in all three yak populations, and might play a major role in local adaptation. Functional enrichment analysis of genes harboring high impact SNPs revealed overrepresented pathways related to response to stress, immune system regulation, and high-altitude adaptation. This study provides comprehensive information about genomic variants and their annotation in Indian yak populations, thus would serve as a data resource for researchers working on the yaks. Furthermore, it could be well exploited for better yak conservation strategies by estimating population genetics parameters <i>viz</i>., effective population size, inbreeding, and observed and expected heterozygosity.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2282723"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138433038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence and molecular characterization of multi-resistant Escherichia coli isolates from clinical bovine mastitis in China. 中国临床牛乳腺炎中分离出的多重耐药大肠埃希菌的流行情况和分子特征。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-03-13 DOI: 10.1080/10495398.2024.2322541
Hongxia Zhao, Hailan Ma, Chen Song, Shuting Fan, Hongliang Fan, Weiguang Zhou, Jinshan Cao
{"title":"Prevalence and molecular characterization of multi-resistant <i>Escherichia coli</i> isolates from clinical bovine mastitis in China.","authors":"Hongxia Zhao, Hailan Ma, Chen Song, Shuting Fan, Hongliang Fan, Weiguang Zhou, Jinshan Cao","doi":"10.1080/10495398.2024.2322541","DOIUrl":"10.1080/10495398.2024.2322541","url":null,"abstract":"<p><p>Different antibiotics are used to treat mastitis in dairy cows that is caused by <i>Escherichia coli (E. coli).</i> Antimicrobial resistance in food-producing animals in China has been monitored since 2000. Surveillance data have shown that the prevalence of multiresistant <i>E. coli</i> in animals has increased significantly. This study aimed to investigate the occurrence and molecular characteristics of resistance determinants in <i>E. coli</i> strains (<i>n</i> = 105) obtained from lactating cows with clinical bovine mastitis (CBM) in China. A total of 220 cows with clinical mastitis, which has swollen mammary udder with reduced and red or gangrenous milk, were selected from 5000 cows. The results showed 94.3% of the isolates were recognized as multidrug resistant. The isolates (30.5%) were positive for the class I integrase gene along with seven gene cassettes that were accountable for resistance to trimethoprim resistance (<i>dfrA17, dfr2d</i> and <i>dfrA1</i>), aminoglycosides resistance (<i>aadA1</i> and <i>aadA5</i>) and chloramphenicol resistance (<i>catB3</i> and <i>catB2</i>), respectively. The <i>bla</i><sub>TEM</sub> gene was present in all the isolates, and these carried the <i>bla</i><sub>CTX</sub> gene. A double mutation in <i>gyrA</i> (i.e., Ser83Leu and Asp87Asn) was observed in all fluoroquinolone-resistant isolates. In total, nine fluoroquinolone-resistant <i>E. coli</i> isolates were identified with five different types of mutations in <i>parC</i>. In four (44.4%) isolates, Ser458Ala was present in parE, and in all nine (9/9) fluoroquinolone-resistant isolates, Pro385Ala was present in gyrB. Meanwhile, fluoroquinolone was observed as highly resistant, especially in isolates with <i>gyrA</i> and <i>parC</i> mutations. In summary, the findings of this research recognize the fluoroquinolone resistance mechanism and disclose integron prevalence and ESBLs in <i>E. coli</i> isolates from lactating cattle with CBM.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2322541"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140118581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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