Animal Biotechnology最新文献_第2页

Enhanced susceptibility of porcine muscle-derived mesenchymal stem cells to Aujeszky's virus compared Vero cells. 与Vero细胞相比，猪肌肉源性间充质干细胞对奥耶斯基病毒的易感性增强。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-20 DOI: 10.1080/10495398.2025.2479677

Na-Yeon Gu, Gwang Sik Ryu, Gyu-Nam Park, Ju-Yeon Lee, Yun Sang Cho, Dong-Kun Yang, Hye Jeong Lee

{"title":"Enhanced susceptibility of porcine muscle-derived mesenchymal stem cells to Aujeszky's virus compared Vero cells.","authors":"Na-Yeon Gu, Gwang Sik Ryu, Gyu-Nam Park, Ju-Yeon Lee, Yun Sang Cho, Dong-Kun Yang, Hye Jeong Lee","doi":"10.1080/10495398.2025.2479677","DOIUrl":"10.1080/10495398.2025.2479677","url":null,"abstract":"Mesenchymal stem cells (MSCs) can self-renew and differentiate into several lineages and can be isolated from different tissues such as bone marrow, adipose tissue, umbilical cord blood, and muscle. Herein, we established MSCs derived from miniature pig muscle (MpMu-MSCs) and assessed their response to Aujeszky's virus. We characterized the MpMu-MSCs based on their cellular morphology, proliferation properties, cell surface marker expression, and mesodermal differentiation potential. MpMu-MSCs demonstrated a fibroblast-like spindle shape and formed a homogeneous monolayer. They showed a considerable increase in cell proliferation over 16 passages. The cells expressed surface markers CD29, CD44, CD90, and CD105 and demonstrated mesodermal lineage differentiation capabilities. MpMu-MSCs demonstrated faster cytopathic effects than the Vero cells when infected with Aujeszky's virus. The virus titer in MpMu-MSCs was initiated at 101.4 TCID50/ml at 12 h post-infection (hpi) and increased to 106.6 TCID50/ml at 72 hpi. In Vero cells, it was initiated at 102.3 TCID50/ml at 48 hpi and increased to 103.8 TCID50/ml at 72 hpi. This study showed that the stem cells procured from miniature pig muscles exhibit MSC characteristics and that the established cells demonstrate higher susceptibility and virus titer to Aujeszky's virus than Vero cells, indicating their potential use in virus research.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2479677"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits. 肌生成抑制素缺乏家兔骨骼肌发育中circrna和lncrna参与的综合分析

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-26 DOI: 10.1080/10495398.2025.2465624

Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan

{"title":"Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits.","authors":"Ling Li, Ting Zhang, Muhammad Farhab, Xiao-Xiao Xia, Abu Musa Md Talimur Reza, Paing Oo Kyaw, Fenglei Chen, Esraa Aly Sayed Ismail, Gang Xue, Ping Zhong, Yong Cheng, Yu-Guo Yuan","doi":"10.1080/10495398.2025.2465624","DOIUrl":"10.1080/10495398.2025.2465624","url":null,"abstract":"Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three WT and four MSTN KO rabbits, we analyzed the expression profiles of circRNAs and lncRNAs. After identifying the differently expressed RNAs, the biological pathways implicated were ascertained by performing enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). We identified differences in the expression of 251 circRNAs (79 upregulated and 172 downregulated), 176 lncRNAs (53 upregulated and 123 downregulated), and 1178 mRNAs (408 upregulated and 770 downregulated) between WT and MSTN KO rabbits. Target genes were significantly enriched in pathways associated with protein synthesis and catabolism, such as oxidative phosphorylation, ubiquitin-mediated proteolysis, the FoxO signaling pathway, and the pentose phosphate pathway, as identified through GO and KEGG enrichment analyses. The constructed network indicates that a class of circRNAs and lncRNAs is engaged in MSTN-mediated regulation of skeletal muscle development. These findings provide valuable insights for innovative therapeutic, diagnostic, and preventive approaches to muscle disorders.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2465624"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eucommia ulmoides and its inhibitory effects on prevotella in piglet gut microbiome through metagenomic and metabolomic analysis. 杜仲及其对仔猪肠道微生物群普氏菌的抑制作用。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/10495398.2025.2503753

Shanshan Han, Qiang Zhang, Hongyan Zhang, Jian Ma

{"title":"Eucommia ulmoides and its inhibitory effects on prevotella in piglet gut microbiome through metagenomic and metabolomic analysis.","authors":"Shanshan Han, Qiang Zhang, Hongyan Zhang, Jian Ma","doi":"10.1080/10495398.2025.2503753","DOIUrl":"10.1080/10495398.2025.2503753","url":null,"abstract":"Eucommia ulmoides (EU) is a traditional medicinal plant widely cultivated across China. The combination of EU and feed significantly affects the growth performance, intestinal microbiota composition, and metabolic characteristics of weaned piglets. Forty Landrace x Yorkshire piglets were randomly assigned to four groups: a control group receiving a basal diet, three treatment groups receiving a basal diet supplemented with EU and EU with mix energy (EU+ME), and EU with high protein and energy (EU+HPE), respectively. Growth performance was monitored over a 25-day feeding period, and fecal samples were collected for subsequent metagenomic sequencing and metabolomic analysis. Piglets supplemented with EU, EU+ME, and EU+HPE exhibited significantly improved growth performance, compared to the control group. Metagenomic analysis revealed significant alterations in gut microbiota composition, with increased beneficial bacterial classes and suppression of Prevotella spp. Metabolomic profiling demonstrated distinct metabolic alterations among the treatment groups, with pathway impact analysis highlighting enhanced protein synthesis and energy metabolism. Furthermore, EU supplementation did not affect porcine epidemic diarrhea virus activity in vitro but reduced LPS-induced intestinal inflammation. These findings suggest that EU could be a promising natural additive for improving piglet health and growth, with potential implications for managing post-weaning challenges in swine production.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503753"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization and insights into the belted coat pattern of a local, reconstituted pig population. 基因组表征和见解到一个地方，重组猪群的带状被毛模式。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/10495398.2025.2515462

Samira Giovannini, Maria Giuseppina Strillacci, Raffaella Milanesi, Caterina Altissimi, Massimo Biagetti, Francesca Maria Sarti

{"title":"Genomic characterization and insights into the belted coat pattern of a local, reconstituted pig population.","authors":"Samira Giovannini, Maria Giuseppina Strillacci, Raffaella Milanesi, Caterina Altissimi, Massimo Biagetti, Francesca Maria Sarti","doi":"10.1080/10495398.2025.2515462","DOIUrl":"10.1080/10495398.2025.2515462","url":null,"abstract":"The Cinghiato pig population originates from a breeding project aimed at reconstituting an extinct local swine breed, historically depicted in frescoes in Umbria, Central Italy. The selection strategy employed a reconstruction breeding program, choosing mating pairs based on the unique coat phenotype represented in these artworks. Unlike the traditional Cinta Senese breed, Cinghiato pigs exhibit a white belt encircling the trunk, while their forelimbs remain black. This study explores the genetic background of the belted coat pattern observed in the heterogeneous reconstituted population. Twenty-two pigs were genotyped using the Porcine GGP 80K SNP BeadChip. Genetic analyses were conducted to assess population structure and diversity, with comparisons made to other Italian pig breeds and wild boars. Findings reveal moderate genetic diversity within the Cinghiato population. Runs of Homozygosity patterns suggest historical inbreeding events. Moreover, several genomic regions were associated with traits relevant to niche pork production, including feed intake, leg conformation, and fat deposition. Polymorphisms were detected in 10 coat color-related genes (KIT, MC1R, ASIP, EDNRB, KITLG, MITF, OCA2, PAX3, SOX10, and TYRP1). Although some candidate variants were identified, this preliminary study highlights the need for further research to clarify the genetic mechanisms underlying the phenotypic variability of belted coat patterns.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2515462"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-genome selective sweep analysis of Danish Large White and Chinese indigenous pig populations. 丹麦大白猪和中国本土猪群的全基因组选择性扫描分析。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-01 DOI: 10.1080/10495398.2025.2467411

Xudong Wu, Decai Xiang, Bofang Duan, Wei Zhang, Mo Li, Yueyun Ding, Zilong Zhao, Guiying Zhao, Zongjun Yin

{"title":"Whole-genome selective sweep analysis of Danish Large White and Chinese indigenous pig populations.","authors":"Xudong Wu, Decai Xiang, Bofang Duan, Wei Zhang, Mo Li, Yueyun Ding, Zilong Zhao, Guiying Zhao, Zongjun Yin","doi":"10.1080/10495398.2025.2467411","DOIUrl":"10.1080/10495398.2025.2467411","url":null,"abstract":"The pig industry is an important component of Chinese agriculture. Chinese indigenous and Western commercial pig breeds provide valuable genetic resources for sustainable development of the pig industry, and selective signal analysis can advance our understanding of artificial and natural selective processes. In this study, we used whole genome re-sequencing data to analyze selection signatures in 43 Danish Large White [LW] pigs and 60 Chinese indigenous pigs (24 Anqing six-end white [AQ], 6 Asian wild [SS] pigs, 15 Diqing Tibetan [DQZ], and 15 Diannan small-ear [DN] pigs). We employed two calculation methods (FST and π ratio) to identify the selection signals of LW and Chinese indigenous pigs (top 1%). Among the selective sweep regions, 15 and 117 candidate genes were identified in Chinese indigenous pigs and LW pigs, respectively. The PIK3IP1, TUG1, and SELENOM genes were related to environmental adaptation in Chinese indigenous pigs; ALDH1A2, APC2, PTBP1, APQ9, and FGF7 were related to reproductive performance in LW pigs. Our findings provide insights into the genetic basis of economic traits of LW and Chinese indigenous pigs and offer a useful reference for future pig breeding and production.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2467411"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145720429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL14 and WTAP play a crucial role in the regulation of bovine preadipocyte differentiation. METTL14和WTAP在牛前脂肪细胞的分化调控中起重要作用。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2476531

Jia Liu, Chicheng Ma, Yu Cheng, Minzhi Wang, Guoqing Zhao, Liwei Huang, Ruigao Song, Xi Wang, Hongxia Li

引用次数: 0

Production of immune receptor knockout chickens via direct in vivo transfection of primordial germ cells. 通过直接在体内转染原始生殖细胞生产免疫受体敲除鸡。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-26 DOI: 10.1080/10495398.2025.2523027

Kristie Jenkins, Daniel Layton, Tamara Gough, Terri O'Neil, Luis Malaver Otega, Ketan Mishra, Kerri Bruce, Kirsten Morris, Terry Wise, Arjun Challagulla, Tim Doran, Andrew Bean

{"title":"Production of immune receptor knockout chickens via direct in vivo transfection of primordial germ cells.","authors":"Kristie Jenkins, Daniel Layton, Tamara Gough, Terri O'Neil, Luis Malaver Otega, Ketan Mishra, Kerri Bruce, Kirsten Morris, Terry Wise, Arjun Challagulla, Tim Doran, Andrew Bean","doi":"10.1080/10495398.2025.2523027","DOIUrl":"10.1080/10495398.2025.2523027","url":null,"abstract":"The advancement of genetic engineering in chickens has enabled significant advancement in developmental biology, bioreactors, and disease resilience. The development of CRISPR/Cas9 genome engineering technology has further expanded the potential applications of genetic engineering in poultry. In this study we aimed to evaluate the efficacy of a direct in vivo transfection method, previously demonstrated to produce transgenic chickens, in generating gene knockout (KO) chickens. Specifically, we targeted the Interferon-α/β Receptor 1 (IFNAR1) and Interleukin 1 receptor, type I (IL1R1), both critical pathways in the inflammatory and antiviral responses. We designed guide RNAs targeting the genes and validated their efficiency in vivo via microinjection into the developing embryos. PCR analysis confirmed the presence of gene deletions in chimeric roosters, which were subsequently bred to produce G1 germline heterozygote KO offspring. Homozygous KO chickens were generated and subjected to phenotypic and functional analyses. Our results demonstrated successful generation of functional knockouts of both IFNAR1 and IL1R1 using a direct in vivo transfection. Overall, this study demonstrates that direct in vivo transfection provides a robust and predictable method for generating KO chickens, facilitating further research into avian immune responses and the development of antiviral strategies.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2523027"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing. 基于全基因组重测序的莆田黑鸭蛋重性状候选基因鉴定

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-17 DOI: 10.1080/10495398.2025.2503754

Yinhua Yang, Weilong Lin, Huihuang Li, Fan Yang, Xinguo Bao, Chengfu Pan, Lianjie Lai, Weimin Lin, Ruiyi Lin

{"title":"Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing.","authors":"Yinhua Yang, Weilong Lin, Huihuang Li, Fan Yang, Xinguo Bao, Chengfu Pan, Lianjie Lai, Weimin Lin, Ruiyi Lin","doi":"10.1080/10495398.2025.2503754","DOIUrl":"10.1080/10495398.2025.2503754","url":null,"abstract":"Egg weight is a primary economic trait in poultry breeding. Putian Black duck, an excellent local laying duck breed in Fujian Province, includes two different strains, black feather strain and white feather strain. The white feather strain of Putian Black duck is also known as Putian White duck. Except for the different feather colors, these two strains differ in egg weight. In this study, whole-genome resequencing was conducted on Putian Black duck and Putian White duck to explore the differences in the genetic mechanism of egg weight. LRP8, VLDLR, and LPL were identified as key candidate genes affecting egg weight. Mass spectrometry was used to detect the SNPs of LRP8, VLDLR, and LPL. Result indicates that the SNPs of LRP8, VLDLR, and LPL in both populations exhibited moderate polymorphism, and Putian Black duck possessed higher genetic variation and potential selectivity. Association analysis indicated that in Putian Black duck, four SNPs in the LRP8 gene were significantly associated with egg weight. These loci can be used as molecular markers for improving egg weight in Putian Black duck.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503754"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key factors causing ketosis in dairy cows with low feed intake. 低采食量奶牛酮症发生的关键因素分析。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-04 DOI: 10.1080/10495398.2025.2487089

Xue Feng, Qi Feng, Sayed Haidar Abbas Raza, Fen Li, Yun Ma

{"title":"Identification of key factors causing ketosis in dairy cows with low feed intake.","authors":"Xue Feng, Qi Feng, Sayed Haidar Abbas Raza, Fen Li, Yun Ma","doi":"10.1080/10495398.2025.2487089","DOIUrl":"10.1080/10495398.2025.2487089","url":null,"abstract":"Ketosis is a common metabolic disease in high-yield dairy cows. Key genes affecting ketosis need to be further explored by new methods. The gene expression profiling and clinical data of GSE92398, GSE104079, and GSE4304 were obtained from the gene expression omnibus (GEO) database. Core modules and genes associated with RFI (residual feed intake) and ADF (alternate day fasting) were identified by weighted gene co-expression network analysis (WGCNA). Subsequently, the key genes related to ketosis and RFI were determined by protein-protein interaction (PPI) networks, ROC curves, functional enrichment, and differential expression analysis, respectively. The results showed that the genes of ACACA, ELOVL6 and XPO7 could be used as regulators of ketosis induced by low feed intake in dairy cows. At the same time, three genes (HRFI, STAT3 and IFNAR1) were retained as additional RFI biomarkers that could be considered. We identified three key factors as candidate genes and biomarkers of ketosis and RFI, respectively. These factors may provide a theoretical basis for targeted therapy of ketosis in dairy cows.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2487089"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep. Kari羊透明带-3 （ZP3）基因的遗传变异及其与产仔数变异的关系

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-24 DOI: 10.1080/10495398.2025.2450364

Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

{"title":"Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.","authors":"Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2450364","DOIUrl":"10.1080/10495398.2025.2450364","url":null,"abstract":"Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets. Additionally, Madakhlasht sheep, which sometimes produce twins, and Balkhi sheep, which produce only singletons, were used as references. The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. Notably, two point mutations and a six-nucleotide insertion were found upstream of the initiation codon in twin-producing Kari ewes, potentially affecting ZP3 expression and LS. Two missense mutations (I101L in exon 1 and R408H in exon 8) were heterozygous in twin-producing Kari ewes but homozygous in other groups, correlating with LS. Protein modelling suggested that the I101L mutation alters the binding site, potentially impacting protein function. These findings indicate that ZP3 gene variations influence reproductive efficiency and LS in sheep, with specific variants serving as potential markers for selective breeding to enhance LS.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450364"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0