Animal Biotechnology最新文献_第2页

The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs. 三七茎叶通过调节都藏猪肠道菌群，降低抗生素耐药基因的丰度。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/10495398.2025.2471785

Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao

{"title":"The stems and leaves of Panax notoginseng reduce the abundance of antibiotic resistance genes by regulating intestinal microbiota in Duzang pigs.","authors":"Wenjie Cheng, Lanlan Yi, Taojie Xu, Yuxiao Xie, Junhong Zhu, Xuancheng Guan, Qiuyan Li, Ying Huang, Yanguang Zhao, Sumei Zhao","doi":"10.1080/10495398.2025.2471785","DOIUrl":"10.1080/10495398.2025.2471785","url":null,"abstract":"In order to study the distribution characteristics of intestinal microbiota and antibiotic resistance genes (ARGs) in Duzang pigs after adding stems and leaves of Panax notoginseng to the feed, the characteristics of intestinal microbiota were explored by metagenomic sequencing, and 14 ARGs and 2 integrase genes were detected by qPCR. The results showed that the addition of stems and leaves of P. notoginseng increased the relative abundance of Firmicutes, Lactobacillus and Pediococcus in the cecum of Duzang pigs. A total of 10 ARGs and 2 integrase genes were detected in the cecal contents of pigs. The addition of stems and leaves of P. notoginseng reduced the relative abundance of total ARGs, ermB, tetO and tetW in the cecum of Duzang pigs. The results of network analysis showed that multiple genera were potential hosts of ARGs. The addition of stems and leaves of P. notoginseng may reduce the relative abundance of ARGs by reducing the relative abundance of genera such as Corynebacterium and Flavonifractor, thereby reducing the risk of ARGs spread. This study provides a theoretical basis for the rational use of stems and leaves of P. notoginseng to control ARGs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2471785"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on immortalization of Mongolian sheep fibroblast cells. 蒙古羊成纤维细胞永生化的研究。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-07 DOI: 10.1080/10495398.2025.2459915

Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao

{"title":"Study on immortalization of Mongolian sheep fibroblast cells.","authors":"Bin Liu, Shichao Wang, Fanhua Meng, Bei Wu, Yanru Zhang, Junwei Cao","doi":"10.1080/10495398.2025.2459915","DOIUrl":"10.1080/10495398.2025.2459915","url":null,"abstract":"This study aims to establish an immortalized fibroblast cell line from Mongolian sheep. Primary Mongolian sheep fibroblasts (SSF) were isolated using tissue explant and enzymatic digestion methods, followed by microscopic observation, growth curve plotting, and karyotype analysis. The results confirmed the successful isolation of SSF. Human (hTERT) and sheep (sTERT) telomerase reverse transcriptase vectors were separately introduced into SSF, with cells passaged up to 36 generations following G418 selection. Microscopic examination and qRT-PCR results demonstrated that TERT transfection did not alter the morphology of SSF and led to stable, high levels of TERT expression (P < 0.01). Cell counting and flow cytometry revealed that TERT-transfected cells had higher viability and lower apoptosis rates compared to SSF (P < 0.05). Karyotype and soft agar colony formation assays indicated that hTERT and sTERT-transfected cells maintained normal characteristics without malignant transformation. β-galactosidase staining indicated that TERT transfection significantly reduced cellular senescence (P < 0.001). Additionally, sTERT-transfected cells exhibited higher TERT expression, enhanced viability, proliferation, and anti-senescence effects compared to hTERT-transfected cells (P < 0.05). In summary, the introduction of hTERT and sTERT effectively extends the lifespan of SSF, with sTERT demonstrating a more pronounced effect. This study provides critical evidence for preserving Mongolian sheep genetic resources and developing immortalized cell lines.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2459915"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep. Kari羊透明带-3 （ZP3）基因的遗传变异及其与产仔数变异的关系

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-24 DOI: 10.1080/10495398.2025.2450364

Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

{"title":"Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.","authors":"Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2450364","DOIUrl":"10.1080/10495398.2025.2450364","url":null,"abstract":"Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets. Additionally, Madakhlasht sheep, which sometimes produce twins, and Balkhi sheep, which produce only singletons, were used as references. The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. Notably, two point mutations and a six-nucleotide insertion were found upstream of the initiation codon in twin-producing Kari ewes, potentially affecting ZP3 expression and LS. Two missense mutations (I101L in exon 1 and R408H in exon 8) were heterozygous in twin-producing Kari ewes but homozygous in other groups, correlating with LS. Protein modelling suggested that the I101L mutation alters the binding site, potentially impacting protein function. These findings indicate that ZP3 gene variations influence reproductive efficiency and LS in sheep, with specific variants serving as potential markers for selective breeding to enhance LS.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450364"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primordial follicle activation related genes screening and differential expressed analysis by transcriptome sequencing in buffalo ovary after PI3K and mTOR stimulators activated. PI3K和mTOR刺激物激活后水牛卵巢原始卵泡激活相关基因筛选及差异表达分析

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-28 DOI: 10.1080/10495398.2025.2510320

Jingyuan Liang, Peng Zhu, Qiao Lv, Yu Pan, Ziyan Gao, Qinghua Xing, Ruimen Zhang, Yanfei Deng, Sufang Yang

{"title":"Primordial follicle activation related genes screening and differential expressed analysis by transcriptome sequencing in buffalo ovary after PI3K and mTOR stimulators activated.","authors":"Jingyuan Liang, Peng Zhu, Qiao Lv, Yu Pan, Ziyan Gao, Qinghua Xing, Ruimen Zhang, Yanfei Deng, Sufang Yang","doi":"10.1080/10495398.2025.2510320","DOIUrl":"https://doi.org/10.1080/10495398.2025.2510320","url":null,"abstract":"Buffaloes are mono-ovulating animals, with only about 5% of primordial follicles being fully capable of maturation into primary oocytes throughout their reproductive years. In vitro primordial follicle activation technology provides a new way to manipulate and utilize oocyte resources. However, in vitro activation of buffalo primordial follicles has not been reported. In this study, buffalo cortical strips were cultured in vitro and activated with PI3K and mTOR stimulators, and the proportion of activated and developed follicles was evaluated and compared between groups . Furthermore, the key genes involved in primordial follicle activation were screened using RNA sequencing. Results showed that buffalo ovarian cortex can be well preserved by being cultured in vitro for at least 7 days and maintain its tissue properties and follicular morphology. In vitro, treatment with PI3K and mTOR pathway stimulators significantly enhanced the activation efficiency of the primordial follicles. In addition, several differentially expressed genes related to follicular development, such as IDO1, CXCL10 and CXCL6 were significantly up-regulated after stimulation. Our findings demonstrate that PI3K and mTOR stimulators can significantly promote the activation and development of buffalo follicles, and providing technical support and a theoretical basis for the optimization of in vitro culture technology of buffalo preantral follicles .","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2510320"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key factors causing ketosis in dairy cows with low feed intake. 低采食量奶牛酮症发生的关键因素分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-04 DOI: 10.1080/10495398.2025.2487089

Xue Feng, Qi Feng, Sayed Haidar Abbas Raza, Fen Li, Yun Ma

引用次数: 0

Transcriptome-wide study of mRNAs modified by m⁶A RNA methylation in the testis development of dairy goats. m6A RNA甲基化修饰的mrna在奶山羊睾丸发育中的转录组研究。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-30 DOI: 10.1080/10495398.2025.2496641

Xinyang Ren, Yingxin Qu, Akang Shari, Guang Li

{"title":"Transcriptome-wide study of mRNAs modified by m6A RNA methylation in the testis development of dairy goats.","authors":"Xinyang Ren, Yingxin Qu, Akang Shari, Guang Li","doi":"10.1080/10495398.2025.2496641","DOIUrl":"https://doi.org/10.1080/10495398.2025.2496641","url":null,"abstract":"N6-methyladenosine (m6A) is an important epigenetic modification in RNA, playing a crucial role in regulating the production and aging of animal testicular sperm. This study extracted mRNA from the testicular tissue of male goats before and after sexual maturity, generating a methylation map through preliminary experiments and methylation immunoprecipitation sequencing. The results showed that during the development of dairy goats, the expression levels of marker genes related to testicular development and methylation-related enzymes changed significantly. A total of 36,602 peaks and 11,223 genes were identified in the two groups, including 2989 differential peaks (427 upregulated and 2562 downregulated) and 1457 differentially expressed genes (833 upregulated and 624 downregulated). The abundance of m6A was positively correlated with gene expression levels. This study reports for the first time the mRNA profiles of m6A modifications across the entire transcriptome during testicular development in Guanzhong dairy goats, providing a new perspective for genetic improvement in goats.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2496641"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying sex-based disparities in porcine mitochondrial function. 确定猪线粒体功能的性别差异。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-10 DOI: 10.1080/10495398.2025.2488068

Hao Liu, Wenshu Shi, Xing Zhang, Xinmiao He, Xingbo Zhao

{"title":"Identifying sex-based disparities in porcine mitochondrial function.","authors":"Hao Liu, Wenshu Shi, Xing Zhang, Xinmiao He, Xingbo Zhao","doi":"10.1080/10495398.2025.2488068","DOIUrl":"https://doi.org/10.1080/10495398.2025.2488068","url":null,"abstract":"In pigs, the effect of sex on production and reproductive traits has been largely reported, however, whether sex exerts its influence through regulating mitochondrial function is still unclear. In this study, we constructed 15 male cells and 15 female fibroblasts derived from 35-day and 50-day fetuses, newborn piglets and 1-year-old pigs to identify the sex effect on mitochondrial functions. Results indicated significant differences on cellular and molecular characteristics between male and female cells, including energy metabolic trait, mitochondrial DNA (mtDNA) replication and transcription, and mRNA expressions of mitochondrial biogenesis genes and mitoprotease genes. Referring to sex, males exhibited significantly higher oxygen consumption rate productions, levels of reactive oxygen species (ROS) and mtDNA copy numbers than those with females in muscle and ear fibroblasts. And the expressions of mtDNA, mitochondrial biogenesis genes (POLG, PPARGC1A, TFAM and TWNK) and XPNPEP3 were higher in males than females in ear fibroblasts derived from 1-year-old adult pigs (EFA cells). While, the cell proliferation and expressions of genes related to ROS metabolism were not influenced by sex. The results highlight the effect of sex on mitochondrial function and gene expression, and provide important data for a comprehensive understanding of the mechanisms underlying sex regulation of energy metabolism-related traits in pigs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2488068"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats. 阴阳补充对奶山羊繁殖性能、抗氧化性和免疫力的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/10495398.2025.2450349

Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai

{"title":"Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats.","authors":"Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai","doi":"10.1080/10495398.2025.2450349","DOIUrl":"10.1080/10495398.2025.2450349","url":null,"abstract":"This study aims to explore the effects of Yin and Yang Double Supplement Compound Chinese Medicine Preparations (YYSBFF) on the reproductive performance, antioxidant levels, and immunity of dairy goats. For the experiment, 36 Alps milk goats were selected and randomly divided into an experimental group and a control group, with 18 goats in each group. The ewes in the experimental group were fed with YYSBFF for 14 d prior to breeding and farrowing. The results of the experiment showed that the estrus rate, embryo acceptance rate, and lamb birth weight in the experimental group were higher than those in the control group, and the weak lamb rate was significantly lower. Moreover, the experimental group exhibited higher levels of reproductive hormones (FSH, LH), antioxidant factors (T-SOD, GSH-Px, MDA), and immunoglobulins (IgA, IgM, IgG) compared to the control group. There were no significant differences in liver and kidney function indicators (ALT, AST, TP, ALB, CREA, UREA) between the experimental and control groups (p > .05). These findings indicate that YYSBFF can enhance the reproductive performance of dairy goats by regulating the level of sex hormones, while also improving the body's antioxidant and immune abilities.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450349"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance. 卵巢和肝脏植物性细胞保护功能的营养基因组学证据为提高蛋鸡生产性能提供了机制支持。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-13 DOI: 10.1080/10495398.2025.2463995

Ioannis Brouklogiannis, Konstantinos C Mountzouris

{"title":"Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance.","authors":"Ioannis Brouklogiannis, Konstantinos C Mountzouris","doi":"10.1080/10495398.2025.2463995","DOIUrl":"10.1080/10495398.2025.2463995","url":null,"abstract":"The study assessed the inclusion level effects of a phytogenic blend (PB) on performance and critical molecular biomarkers related to detoxification (Aryl hydrocarbon receptor; AhR) and antioxidant (Nuclear factor-erythroid 2-related factor 2; Nrf2) responses in layers' ovary and liver. Layers (n = 385; 21-week-old; Hy-Line Brown) were allotted to 5 treatments with 7 replicates of 11 hens each, for a 12-week feeding trial. Treatments were: control (CON) without PB or supplementation with PB at 250 (PB250), 750 (PB750), 1000 (PB1000) and 1500 mg/kg diet (PB1500), respectively. Performance was determined weekly for the 12-week experimental period. At the 6th and 12th experimental week ovarian and liver samples were collected for gene expression analysis. Increasing PB inclusion level, improved linearly and quadratically overall laying rate, egg mass and FCR, with optimal (P ≤ 0.001) performance notable in the PB750 compared to CON. The nutrigenomic analysis revealed that PB inclusion resulted (P ≤ 0.05) in consistent beneficial modulation of the AhR/Nrf2 pathway-related genes assessed at the 6th and 12th experimental week, both in the ovary and the liver of laying hens. In conclusion, PB beneficially modulated the ovarian and hepatic adaptive cytoprotection and supported the laying performance improvements recorded, with PB750 displaying the optimal benefits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2463995"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peony seed meal supplementation enhances semen quality in aged Qinchuan bulls. 添加牡丹籽粕可提高秦川老年公牛精液质量。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-17 DOI: 10.1080/10495398.2025.2503752

Shaojie Cao, Shiwei Li, Kaixue Lu, Juntai Fu, Chengwei Yang, Jiahui Qu, Juanjuan Li, Xianlin Zhao

{"title":"Peony seed meal supplementation enhances semen quality in aged Qinchuan bulls.","authors":"Shaojie Cao, Shiwei Li, Kaixue Lu, Juntai Fu, Chengwei Yang, Jiahui Qu, Juanjuan Li, Xianlin Zhao","doi":"10.1080/10495398.2025.2503752","DOIUrl":"https://doi.org/10.1080/10495398.2025.2503752","url":null,"abstract":"Healthy Qinchuan bulls aged 8.5-9.5 years were selected and randomly divided into 6 groups based on 0%, 2%, 4%, 6%, 8%, and 10% dietary supplementation of peony seed meal for three months. Overall, linear motility (LM), progressive motility (PM), straight-line velocity (VSL), curvilinear velocity (VCL), and the percentage increase of all sperm grades (A to D) were affected (p<0.05) by the dietary supplementation. LM (29.5 vs. 24.1%), PM (69.4 vs. 60.0%), VLS (38.0 vs. 33.3 μm/s), and VCL (48.3 vs. 44.3 μm/s) were consistently higher in the 6% peony seed meal dietary supplementation groups than in the control group, respectively. A similar pattern was observed in the 8% group. Inversely to sperm grades C and D, grades A and B were higher in all treated groups than the control group, except for the 2% group in sperm grade A. Moreover, the levels of the enzymes Superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR; except for 2% peony seed group), and glutathione peroxidase (GSH-px) were higher than the control group. The level of malondialdehyde (MDA) was lower in all treated groups than in the control group. Dietary supplementation of 2-8% peony seed meal improves the semen quality of aged Qinchuan bulls.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503752"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0