Kari羊透明带-3 (ZP3)基因的遗传变异及其与产仔数变异的关系

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-24 DOI:10.1080/10495398.2025.2450364
Izaz Ali, Muhammad Ibrahim, Sohail Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Ibrahim A Alhidary, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni
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引用次数: 0

摘要

绵羊产仔数(LS)的变化与遗传因素有关,包括透明带-3 (ZP3)基因,该基因在绵羊的繁殖过程中起作用。本研究探讨了Kari羊ZP3基因变异与LS的关系。研究人员分析了两组160只Kari母羊:一组一直产单胎,另一组产双胞胎,偶尔产三胞胎。此外,有时产双胞胎的Madakhlasht羊和只产单胎的Balkhi羊被用作参考。用PCR扩增整个ZP3基因,用Next Generation Sequencing进行30倍测序。生物信息学分析确定了三个品种的70个变异,位于上游区域,内含子和外显子。值得注意的是,在双生Kari母羊的起始密码子上游发现了两个点突变和一个6个核苷酸的插入,这可能会影响ZP3的表达和LS。两个错义突变(外显子1的I101L和外显子8的R408H)在产双的Kari母羊中是杂合的,而在其他群体中是纯合的,与LS相关。蛋白质模型表明,I101L突变改变了结合位点,可能影响蛋白质功能。这些结果表明,ZP3基因的变异影响绵羊的繁殖效率和LS,特定的变异可以作为选择性育种提高LS的潜在标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.

Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets. Additionally, Madakhlasht sheep, which sometimes produce twins, and Balkhi sheep, which produce only singletons, were used as references. The entire ZP3 gene was amplified using PCR and sequenced at 30× with Next Generation Sequencing. Bioinformatics analysis identified 70 variants across the three breeds, located in upstream regions, introns, and exons. Notably, two point mutations and a six-nucleotide insertion were found upstream of the initiation codon in twin-producing Kari ewes, potentially affecting ZP3 expression and LS. Two missense mutations (I101L in exon 1 and R408H in exon 8) were heterozygous in twin-producing Kari ewes but homozygous in other groups, correlating with LS. Protein modelling suggested that the I101L mutation alters the binding site, potentially impacting protein function. These findings indicate that ZP3 gene variations influence reproductive efficiency and LS in sheep, with specific variants serving as potential markers for selective breeding to enhance LS.

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来源期刊
Animal Biotechnology
Animal Biotechnology 工程技术-奶制品与动物科学
CiteScore
2.90
自引率
5.40%
发文量
230
审稿时长
>12 weeks
期刊介绍: Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes
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