Animal Biotechnology最新文献_第3页

Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats. 阴阳补充对奶山羊繁殖性能、抗氧化性和免疫力的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/10495398.2025.2450349

Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai

{"title":"Effects of Yin and Yang supplement on reproductive performance, antioxidant and immunity of dairy goats.","authors":"Kang Wang, Zhi Yang, Fumei Yang, Guanzong Li, Yulin Sun, Gang Duan, Jun He, Wang Sun, Ke Zhou, Zhihao Xiong, Feiyan Dai","doi":"10.1080/10495398.2025.2450349","DOIUrl":"10.1080/10495398.2025.2450349","url":null,"abstract":"This study aims to explore the effects of Yin and Yang Double Supplement Compound Chinese Medicine Preparations (YYSBFF) on the reproductive performance, antioxidant levels, and immunity of dairy goats. For the experiment, 36 Alps milk goats were selected and randomly divided into an experimental group and a control group, with 18 goats in each group. The ewes in the experimental group were fed with YYSBFF for 14 d prior to breeding and farrowing. The results of the experiment showed that the estrus rate, embryo acceptance rate, and lamb birth weight in the experimental group were higher than those in the control group, and the weak lamb rate was significantly lower. Moreover, the experimental group exhibited higher levels of reproductive hormones (FSH, LH), antioxidant factors (T-SOD, GSH-Px, MDA), and immunoglobulins (IgA, IgM, IgG) compared to the control group. There were no significant differences in liver and kidney function indicators (ALT, AST, TP, ALB, CREA, UREA) between the experimental and control groups (p > .05). These findings indicate that YYSBFF can enhance the reproductive performance of dairy goats by regulating the level of sex hormones, while also improving the body's antioxidant and immune abilities.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2450349"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance. 卵巢和肝脏植物性细胞保护功能的营养基因组学证据为提高蛋鸡生产性能提供了机制支持。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-02-13 DOI: 10.1080/10495398.2025.2463995

Ioannis Brouklogiannis, Konstantinos C Mountzouris

{"title":"Nutrigenomic evidence of phytogenic cytoprotective functions in the ovary and liver provides mechanistic support for improved laying hen performance.","authors":"Ioannis Brouklogiannis, Konstantinos C Mountzouris","doi":"10.1080/10495398.2025.2463995","DOIUrl":"10.1080/10495398.2025.2463995","url":null,"abstract":"The study assessed the inclusion level effects of a phytogenic blend (PB) on performance and critical molecular biomarkers related to detoxification (Aryl hydrocarbon receptor; AhR) and antioxidant (Nuclear factor-erythroid 2-related factor 2; Nrf2) responses in layers' ovary and liver. Layers (n = 385; 21-week-old; Hy-Line Brown) were allotted to 5 treatments with 7 replicates of 11 hens each, for a 12-week feeding trial. Treatments were: control (CON) without PB or supplementation with PB at 250 (PB250), 750 (PB750), 1000 (PB1000) and 1500 mg/kg diet (PB1500), respectively. Performance was determined weekly for the 12-week experimental period. At the 6th and 12th experimental week ovarian and liver samples were collected for gene expression analysis. Increasing PB inclusion level, improved linearly and quadratically overall laying rate, egg mass and FCR, with optimal (P ≤ 0.001) performance notable in the PB750 compared to CON. The nutrigenomic analysis revealed that PB inclusion resulted (P ≤ 0.05) in consistent beneficial modulation of the AhR/Nrf2 pathway-related genes assessed at the 6th and 12th experimental week, both in the ovary and the liver of laying hens. In conclusion, PB beneficially modulated the ovarian and hepatic adaptive cytoprotection and supported the laying performance improvements recorded, with PB750 displaying the optimal benefits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2463995"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peony seed meal supplementation enhances semen quality in aged Qinchuan bulls. 添加牡丹籽粕可提高秦川老年公牛精液质量。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-17 DOI: 10.1080/10495398.2025.2503752

Shaojie Cao, Shiwei Li, Kaixue Lu, Juntai Fu, Chengwei Yang, Jiahui Qu, Juanjuan Li, Xianlin Zhao

{"title":"Peony seed meal supplementation enhances semen quality in aged Qinchuan bulls.","authors":"Shaojie Cao, Shiwei Li, Kaixue Lu, Juntai Fu, Chengwei Yang, Jiahui Qu, Juanjuan Li, Xianlin Zhao","doi":"10.1080/10495398.2025.2503752","DOIUrl":"https://doi.org/10.1080/10495398.2025.2503752","url":null,"abstract":"Healthy Qinchuan bulls aged 8.5-9.5 years were selected and randomly divided into 6 groups based on 0%, 2%, 4%, 6%, 8%, and 10% dietary supplementation of peony seed meal for three months. Overall, linear motility (LM), progressive motility (PM), straight-line velocity (VSL), curvilinear velocity (VCL), and the percentage increase of all sperm grades (A to D) were affected (p<0.05) by the dietary supplementation. LM (29.5 vs. 24.1%), PM (69.4 vs. 60.0%), VLS (38.0 vs. 33.3 μm/s), and VCL (48.3 vs. 44.3 μm/s) were consistently higher in the 6% peony seed meal dietary supplementation groups than in the control group, respectively. A similar pattern was observed in the 8% group. Inversely to sperm grades C and D, grades A and B were higher in all treated groups than the control group, except for the 2% group in sperm grade A. Moreover, the levels of the enzymes Superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR; except for 2% peony seed group), and glutathione peroxidase (GSH-px) were higher than the control group. The level of malondialdehyde (MDA) was lower in all treated groups than in the control group. Dietary supplementation of 2-8% peony seed meal improves the semen quality of aged Qinchuan bulls.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2503752"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in conventional and non-conventional semen characteristics of selected rabbit breeds. 选定兔种常规和非常规精液特征的变化。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-08-22 DOI: 10.1080/10495398.2025.2548300

Eva Tvrdá, Filip Benko, Michal Ďuračka, Anton Kováčik, Jakub Vozaf, Andrea Svoradová, Jaromír Vašíček, Andrej Baláži, Peter Supuka, Simona Kunová, Jana Žiarovská, Miroslava Kačániová, Peter Chrenek

{"title":"Variations in conventional and non-conventional semen characteristics of selected rabbit breeds.","authors":"Eva Tvrdá, Filip Benko, Michal Ďuračka, Anton Kováčik, Jakub Vozaf, Andrea Svoradová, Jaromír Vašíček, Andrej Baláži, Peter Supuka, Simona Kunová, Jana Žiarovská, Miroslava Kačániová, Peter Chrenek","doi":"10.1080/10495398.2025.2548300","DOIUrl":"https://doi.org/10.1080/10495398.2025.2548300","url":null,"abstract":"This article strove to characterize and compare biochemical, oxidative, bacteriological and immunological semen properties in Zemplin, Liptov Baldspotted and New Zealand rabbits. Besides, bacteria were characterized using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oxidative profile of semen was assessed using chemiluminescent and colorimetric protocols. Levels of pro-inflammatory cytokines were evaluated with the enzyme-linked immunosorbent assay. Seminal plasma biochemistry was assessed with an automated clinical chemistry analyser. The lowest sperm concentration and motility were found in the Liptov Baldspotted ejaculates which also presented with significantly (p < 0.01) elevated levels of tumour necrosis factor alpha (TNF-α) and interleukin 1, free radicals (p < 0.0001) and malondialdehyde (p < 0.0001) in comparison to New Zealand rabbits. The prevailing bacterial genera in semen were Stenotrophomonas spp., Staphylococcus spp., Micrococcus spp. and Acinetobacter spp. Significantly increased levels of alanine transaminase and creatinine (Crea) were found in New Zealand rabbits in comparison to the Liptov Baldspotted breed (p < 0.001). Overall, Liptov Baldspotted rabbits produced semen of lower quality than Zemplin and New Zealand rabbits, suggesting that this breed may be more predisposed to a higher susceptibility to internal and external stresses which may interfere with male fertility.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2548300"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel polymorphism at ARHGAP24 gene and its association with growth traits in Hu sheep. 湖羊ARHGAP24基因新多态性及其与生长性状的关系。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-06-11 DOI: 10.1080/10495398.2025.2513958

Huili Shan, Liang Yong Guo, Xin Huang, Xiaowei Zhang, Yongqing Jiang, Sangang He, Junfang Jiang

{"title":"Novel polymorphism at ARHGAP24 gene and its association with growth traits in Hu sheep.","authors":"Huili Shan, Liang Yong Guo, Xin Huang, Xiaowei Zhang, Yongqing Jiang, Sangang He, Junfang Jiang","doi":"10.1080/10495398.2025.2513958","DOIUrl":"https://doi.org/10.1080/10495398.2025.2513958","url":null,"abstract":"It is essential to identify potential genes and genetic variants associated with growth traits to enhance sheep breeding. Previous studies have suggested that the Rho GTPase-activating protein 24 (ARHGAP24) gene may influence the growth of some animals. However, no association has been established between polymorphisms in the ARHGAP24 gene and sheep growth traits. In this study, single-nucleotide polymorphisms (SNPs) in the ARHGAP24 gene of Hu sheep were detected, and functional SNPs associated with Hu sheep growth traits were identified. Twenty-six SNPs were discovered in Hu sheep. Association analysis revealed NC_056059.1:g.455981A > G was significantly associated with average daily gain from six months to one year of age, while NC_056059.1:g.456083 A > G was significantly associated with average daily gain from weaning to six months of age. The relative fluorescence activity (firefly luciferase and sea kidney luciferase) of haplotype GGGG was significantly lower than that of haplotypes GGAA, AAAA and AAGG. Furthermore, ARHGAP24 mRNA expression in the longissimus dorsi muscle of six-months-old sheep was significantly lower than birth sheep. The results showed that NC_056059.1:g.455981A > G and g NC_056059.1:g.456083A > G of ARHGAP24 gene were related to some growth traits of Hu sheep, and ARHGAP24 mRNA was differentially expressed in longissimus dorsi muscle of Hu sheep at different months of age, which could be used as a candidate gene for molecular marker-assisted selection of Hu sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2513958"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candidate gene search for milk production and composition, milk coagulation properties, and milk protein profile in dairy sheep from a New Zealand flock. 候选基因搜索的牛奶产量和组成，牛奶凝固特性，牛奶蛋白谱从新西兰羊群。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-11 DOI: 10.1080/10495398.2025.2516688

A C Marshall, N Lopez-Villalobos, V Vigolo, M De Marchi, S M Loveday, M Weeks, W McNabb

{"title":"Candidate gene search for milk production and composition, milk coagulation properties, and milk protein profile in dairy sheep from a New Zealand flock.","authors":"A C Marshall, N Lopez-Villalobos, V Vigolo, M De Marchi, S M Loveday, M Weeks, W McNabb","doi":"10.1080/10495398.2025.2516688","DOIUrl":"https://doi.org/10.1080/10495398.2025.2516688","url":null,"abstract":"The objective of this study was to perform genome-wide association analysis and thus search for candidate genes for milk production and composition, milk coagulation properties (MCP), and milk protein profile in dairy sheep from a New Zealand flock. After quality control, 45,801 single nucleotide polymorphisms (SNPs) were included in the analysis, 147 ewes, and 470 individual records. A total of 87 SNPs and 55 candidate genes were found across Ovis aries autosomes (OAR) 2, 3, 6, 16, 18, 20, 25, and 26. Of particular importance, were the candidate genes PDZRN4 for milk yield, and BMP2K for contents of αs1- and αs2-caseins. No SNPs were found in the casein genes, LALBA or PAEP. Only one SNP was significant for MCP, and overall, the genetic architecture of MCP was similar to that of ratio of casein to calcium, pH, lactose, and the ratio of casein to protein. Further studies with larger flocks and with genomic imputation are required to validate the findings of this study before incorporating markers or genes into breeding programmes.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2516688"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples. 使用氮同位素分馏作为猪血液和毛发样本饲料转化效率的生物标志物。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI: 10.1080/10495398.2025.2473494

Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki

{"title":"The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples.","authors":"Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki","doi":"10.1080/10495398.2025.2473494","DOIUrl":"10.1080/10495398.2025.2473494","url":null,"abstract":"This study aimed to 1) Establish relationships between feed conversion efficiency (FCE; live weight gain/intake) and several biomarkers in pigs using blood and hair samples, and 2) Investigate the relative FCE performance of pigs from maternal vs. terminal genetic lines fed high vs. low energy diets. 80 male pigs (Large White x Landrace) were fed for 56 days. The terminal genetic line and pigs fed the high energy diet had 5% and 15% higher FCE than the maternal genetic line and pigs fed the low energy diet, respectively. Blood nitrogen isotopic fractionation (Δ15N; animal δ15N - feed δ15N) explained 34% more variation of FCE, compared with the blood insulin‑like growth factor‑1 (IGF‑1). The turnover rate of δ15N in plasma was faster than δ15N in blood, suggesting that blood and plasma δ15N can predict long‑term versus short‑term FCE changes. Pigs fed the high energy diets showed 13% higher live weight gain, 16% greater high standard carcass weight, and 38% higher carcass backfat than those on low‑energy diets. In conclusion, Δ15N is a more effective FCE biomarker for pigs compared to IGF‑1. Hair samples were less predictive of FCE than blood‑derived biomarkers, suggesting further refinement in the methodology of harvesting hair samples.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2473494"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between the expression levels of myogenic regulatory factor genes and carcass characteristics in Kivircik and Hungarian Merino lambs. 基维尔西克和匈牙利美利奴羔羊产肌调节因子基因表达水平与胴体性状的关系。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-23 DOI: 10.1080/10495398.2025.2479690

Korhan Arslan, Fadime Daldaban, Hulya Yalcintan, Pembe Dilara Kecici, Bekir Ozturk, Bulent Ekiz, Bilal Akyuz

{"title":"Relationship between the expression levels of myogenic regulatory factor genes and carcass characteristics in Kivircik and Hungarian Merino lambs.","authors":"Korhan Arslan, Fadime Daldaban, Hulya Yalcintan, Pembe Dilara Kecici, Bekir Ozturk, Bulent Ekiz, Bilal Akyuz","doi":"10.1080/10495398.2025.2479690","DOIUrl":"10.1080/10495398.2025.2479690","url":null,"abstract":"This study aimed to investigate the expression profiles of the myogenic regulatory genes MYOD1, MYOG, MYF5, MYF6, and MSTN in longissimus dorsi muscle, as well as the correlation of the expression levels of these genes with carcass characteristics and growth performance in the Kivircik and Hungarian Merino sheep breeds. The expression levels of the MYF5, MYF6, and MYOG genes were found to be significantly correlated with the rib proportion, the expression level of the MYOG gene was identified as being the main determinant of variations in the rib proportion in the Kivircik lambs. The regression analysis results revealed that the expression levels of the MYF5 and MSTN genes played an essential role in determining the cold carcass dressing percentage in Hungarian Merino lambs. Further, as a result of the regression analysis, the model including the expression level of the MYF6 gene demonstrated that this gene could be responsible for 36.4% of the differences observed in cold carcass weight. In conclusion, the findings of this study suggest that the expression levels of the MYF5, MYF6, and MYOG genes were associated with various carcass traits, particularly in the Kivircik breed, and these genes hold potential as markers for enhancing breed productivity.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2479690"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology of bovine leukemia virus in cattle and phylogenetic analysis for determining its prevailing genotype in Khyber Pukhtunkhwa, Pakistan. 巴基斯坦开伯尔普赫图赫瓦省牛白血病病毒的分子流行病学和确定其流行基因型的系统发育分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-04-17 DOI: 10.1080/10495398.2025.2486029

Farida Tahir, Umer Sadique, Farkhanda Tahir, Mikhlid H Almutairi, Abdulwahed Fahad Alrefaei, Shabana Naz, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni

{"title":"Molecular epidemiology of bovine leukemia virus in cattle and phylogenetic analysis for determining its prevailing genotype in Khyber Pukhtunkhwa, Pakistan.","authors":"Farida Tahir, Umer Sadique, Farkhanda Tahir, Mikhlid H Almutairi, Abdulwahed Fahad Alrefaei, Shabana Naz, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2486029","DOIUrl":"https://doi.org/10.1080/10495398.2025.2486029","url":null,"abstract":"This research focused on assessing the molecular prevalence of Bovine Leukemia Virus (BLV) in different cattle farms throughout Khyber Pakhtunkhwa and characterizing the dominant BLV genotypes by analyzing partial sequences of the gp51 gene. A total of 1,250 blood samples were collected from cattle of both sexes, various age groups (<1 year, 1-3 years, 3-5 years, and >5 years), and different breeds (Friesian, Jersey, Sahiwal, Achai, and crossbred) from multiple cattle farms. Of the 1,250 samples tested, BLV was detected in 136 (10.88%) using nested PCR. Risk factor analysis revealed a significantly higher prevalence of BLV in exotic breeds and older cattle. To confirm the findings and genotype the BLV isolates, four PCR-positive samples were sequenced. Phylogenetic analysis identified the isolates as belonging to genotype I, closely related to GI BLV isolates from Japan. Furthermore, the isolates in this study formed a tightly clustered group, suggesting a common origin from an earlier virus introduced into the host population in the study area.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2486029"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality and microbial community analysis of solid-state fermented feed with mixed bacteria from corn silage. 玉米青贮混合菌固态发酵饲料品质及微生物群落分析。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-05-29 DOI: 10.1080/10495398.2025.2507905

Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao

{"title":"Quality and microbial community analysis of solid-state fermented feed with mixed bacteria from corn silage.","authors":"Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao","doi":"10.1080/10495398.2025.2507905","DOIUrl":"https://doi.org/10.1080/10495398.2025.2507905","url":null,"abstract":"The efficacy of microbial fermented feeds (FF) is highly dependent on microbial composition, underscoring the need for locally sourced, cost-effective formulations to advance sustainable livestock production. In this study, bacteria from corn silage (CSB) were screened for the preparation of solid-state fermented feed (SFF). In addition, for the purpose of comparison, purchased marketed fermentation bacteria (PMB) for SFF were also tested using the same methodology. Anaerobic fermentation was carried out by adding 5% CSB, PMB, and saline to conventional feeds at 37 °C, respectively. Then 10 g of fermentation samples was collected on days 0, 3, and 5, respectively. The composition and diversity of the microbial community were subsequently analyzed using high-throughput sequencing. The pH, titratable acidity (TA), and nutrient index of the feeds before and after fermentation were determined. High-throughput sequencing results showed that the dominant genera of CSB were Weissella, Pseudomonas, and Bacteroidetes, whereas the dominant genera of PMB were Bacteroidetes, Bacillus, Pseudomonas, and Enterococcus. The dominant genera in unfermented feeds (CK) were Chlorophyta, Mitochondria, and Pseudomonas. In addition, the dominant genera in SFF supplemented with CSB and PMB were Weissella and Bacteroidetes, respectively. Compared with CK, pH of SFF supplemented with CSB and PMB decreased gradually, and TA increased significantly and then decreased gradually during fermentation. Similarly, Crude fiber content was higher than CK in both CSB and PMB, while crude fat content was lower than CK for all of them. The study illustrates that CSB can be used to ferment porcine solid feeds and that CSB solid feed microorganisms may promote intestinal absorption of complex polysaccharides in pigs, which lays a certain theoretical foundation for the application of CSB to SFF.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2507905"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0