贾尼牦牛瘤胃菌群的组成和多样性:微生物生态学和功能研究

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Biotechnology Pub Date : 2025-12-01 Epub Date: 2025-03-12 DOI:10.1080/10495398.2025.2476539
Yangji Cidan, Jia Wang, Hongzhuang Wang, Chang Xu, Yanbin Zhu, Muhammad Kasib Khan, Wangdui Basang, Kun Li
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引用次数: 0

摘要

本研究旨在利用ITS测序技术探讨14 - (PLf)和15 - rib (DLf)贾尼牦牛瘤胃真菌菌群的多样性和功能。PLf和DLf组分别获得1,079,105和1,086,799个筛选序列,其中491个asv为两者共有。两组菌群α -多样性无显著差异。而β -多样性分析表明,PLf组中有15个属的丰度与DLf组中有2个属的丰度存在显著差异(p <;0.05)。16S rRNA测序结果显示,在门水平上,14头肋牦牛的子囊菌群、担子菌群和橄榄菌群存在显著差异,而15头肋牦牛的Neocallimastigomycota、Mortierellomycota和Rozellomycota存在显著差异(p)。在属水平上,14头肋牦牛的红霉菌属(Rhodotorula)、Kluyveromyces、Comoclathris、Arthrinium、Cladophialophora、Seimatosporium、Lambertella和Sphacelotheca, 15头肋牦牛的Orpinomyces、Ustilago、Fusarium、Aspergillus、Caecomyces、Alternaria、Trichoderma和Acremonium差异显著(p)。基于15肋牦牛(DLf)瘤胃真菌DNA序列的预测功能分析表明,参与能量代谢的基因上调。本研究为遗传变异如何影响贾尼牦牛肠道菌群提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Composition and diversity of rumen mycobiota in Jiani yaks (Bos grunniens jiani): insights into microbial ecology and functions.

This study aimed to explore the diversity and functions of rumen mycobiota in 14‑ (PLf) and 15‑rib (DLf) Jiani yaks using ITS sequencing. A total of 1,079,105 and 1,086,799 filtered sequences were obtained for the PLf and DLf groups, respectively, with 491 ASVs common to both. No significant difference regarding the α‑diversity of mycobiota within the two groups was observed. While β‑diversity analysis indicated that the abundance of fifteen (15) genera in the PLf group and two (2) genera in the DLf group was found to be significantly different (p < 0.05). 16S rRNA sequencing results indicated that at the phylum level, in 14 ribs yaks Ascomycota, Basidiomycota, and Olpidiomycota, while in 15 rib yaks, Neocallimastigomycota, Mortierellomycota, and Rozellomycota were found to be significantly different (p < 0.05). At the genus level, Rhodotorula, Kluyveromyces, Comoclathris, Arthrinium, Cladophialophora, Seimatosporium, Lambertella, and Sphacelotheca in 14 rib yaks, and Orpinomyces, Ustilago, Fusarium, Aspergillus, Caecomyces, Alternaria, Trichoderma and Acremonium in 15 rib yaks were found to be significantly (p < 0.05) different. Predictive functional analysis based on ruminal fungal DNA sequences from 15‑rib yaks (DLf) demonstrated that genes involved in energy metabolism were upregulated. This study sheds novel insights into how genetic variations influence gut mycobiota in Jiani yak.

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来源期刊
Animal Biotechnology
Animal Biotechnology 工程技术-奶制品与动物科学
CiteScore
2.90
自引率
5.40%
发文量
230
审稿时长
>12 weeks
期刊介绍: Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes
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