Animal Biotechnology最新文献_第10页

Caffeic acid improves intestinal barrier functions by regulating colonic bacteria and tight junction protein expression and alleviating inflammation in weaning piglets. 咖啡酸通过调节结肠细菌和紧密连接蛋白的表达，改善肠道屏障功能，缓解断奶仔猪的炎症。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-04-17 DOI: 10.1080/10495398.2023.2200441

Xiaoling Chen, Tianlei Qiao, Zhengyu Mao, Gang Jia, Hua Zhao, Guangmang Liu, Zhiqing Huang

{"title":"Caffeic acid improves intestinal barrier functions by regulating colonic bacteria and tight junction protein expression and alleviating inflammation in weaning piglets.","authors":"Xiaoling Chen, Tianlei Qiao, Zhengyu Mao, Gang Jia, Hua Zhao, Guangmang Liu, Zhiqing Huang","doi":"10.1080/10495398.2023.2200441","DOIUrl":"10.1080/10495398.2023.2200441","url":null,"abstract":"The experiment investigated the effect of caffeic acid on bacteria, short-chain fatty acids (SCFA), and the expression of tight junction protein and inflammation related genes in the colon of weaning piglets. Thirty-six weaning piglets were allocated to three treatment groups, which were fed with a basal diet, a basal diet supplemented with 250 mg/kg or 500 mg/kg caffeic acid for 28 days. The results showed that caffeic acid treatment increased the contents of acetate acid, propionate acid and total SCFA. Moreover, real-time quantitative PCR showed that the number of Bifidobacterium (p < 0.05) and Lactobacillus (p < 0.05) were increased and the number of Escherichia coli (p < 0.05) was decreased by caffeic acid in colonic mucosa. Real-time quantitative PCR also showed that the mRNA levels of zonula occludens-1 (p < 0.01), claudin-1 (p < 0.01), occludin (p < 0.01), mucin 1 (MUC1) (p < 0.01), MUC2 (p < 0.01), interleukin 4 (IL-4) (p < 0.01) and IL-10 (p < 0.05) were increased, while the mRNA expression levels of histone deacetylases (p < 0.01), IL-1 (p < 0.01), IL-6 (p < 0.01) and tumor necrosis factor-α (TNF-α) (p < 0.01) were decreased, by caffeic acid in colonic mucosa. These results suggested that caffeic acid could improve intestinal barrier function in weaned pigs, which might be mediated by regulating colonic bacteria and tight junction protein expression and alleviating inflammation.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"3693-3699"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9310624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary zinc-chitosan nanoparticles addition influences on growth performance, apparent total tract digestibility, carcass indices, and immune function in weaned rabbits. 日粮中添加纳米锌壳聚糖对断奶兔生长性能、表观总消化率、胴体指数和免疫功能的影响

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-04-13 DOI: 10.1080/10495398.2023.2197467

Fawzia A Hassan, Nabila E M Elkassas, E A El-Bltagy, Manal S Mohamed, Samia Mobarez, Ibrahim H Salim, M M Abdel-Aal

{"title":"Dietary zinc-chitosan nanoparticles addition influences on growth performance, apparent total tract digestibility, carcass indices, and immune function in weaned rabbits.","authors":"Fawzia A Hassan, Nabila E M Elkassas, E A El-Bltagy, Manal S Mohamed, Samia Mobarez, Ibrahim H Salim, M M Abdel-Aal","doi":"10.1080/10495398.2023.2197467","DOIUrl":"10.1080/10495398.2023.2197467","url":null,"abstract":"The aim of this study was to study the effect of zinc oxide-chitosan nanoparticles (Zn-CNPs) on growth performance, plasma constituents, carcass indices, and immune function in rabbit diets. Eighty weaned V-line males rabbits at 5 weeks of age were divided into four dietary groups of control diet; 50 Zn-CNPs; 75 Zn-CNPs;100 Zn-CNPs. Supplementation of Zn-CNPs did not affect final live body weight and average daily weight gain. 100 ppm Zn-CNPs recorded (p < 0.05) higher digestibility of DM, OM, CP, EE, and NFE. Dietary groups of 75 and 100 ppm were higher (p < 0.05) in DCP, TDN (%), and DE (kcal/kg). Zn-CNPs supplementation was higher (p < 0.05) in hot carcass and spleen weights %. The addition of Zn-CNPs in diets promoted dressing%. Significant increases (p < 0.05) in plasma total protein and albumin levels for 75 and 100 Zn-CNPs. Zn-CNPs decreased (p < 0.05) glucose, total cholesterol, LDL concentrations and increased (p < 0.05) plasma zinc levels, IgG, IgM. Plasma HDL level increased (p < 0.05) with 75 and 100 ppm Zn-CNPs. In conclusion, Zn-CNPs supplementations can use safely as a zinc source in rabbits diets without any detrimental effects on growth performance, plasma constituents, and carcass indices. Moreover, 50, 75, and 100 ppm Zn-CNPs enhanced the immune functions.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"4819-4827"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9343335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A missense mutation of the WNK1 gene affects cold tolerance in Chinese domestic cattle. WNK1基因的错义突变影响中国家牛的耐寒性

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-04-20 DOI: 10.1080/10495398.2023.2196316

Xin Liu, Jialei Chen, Xinlong Xu, Jianyong Liu, Jicai Zhang, Haijian Cheng, Zulfiqar Ahmed, Bizhi Huang, Chuzhao Lei

{"title":"A missense mutation of the WNK1 gene affects cold tolerance in Chinese domestic cattle.","authors":"Xin Liu, Jialei Chen, Xinlong Xu, Jianyong Liu, Jicai Zhang, Haijian Cheng, Zulfiqar Ahmed, Bizhi Huang, Chuzhao Lei","doi":"10.1080/10495398.2023.2196316","DOIUrl":"10.1080/10495398.2023.2196316","url":null,"abstract":"Inclement weather conditions, especially cold stress, have threatened the cattle industry. Cattle exposed to cold environments for a longer time suffer developmental delay, immunity decline, and eventually death. WNK1 is a member of With-no-lysine kinases (WNKs), widely expressed in animal organs and tissues. WNK1 and WNK4 are expressed in adipose tissue, and WNK4 promotes adipogenesis. WNK1 does not directly affect adipogenesis but has been shown to promote WNK4 expression in several tissues or organs. One missense mutation NC_037346.1:g.107692244, A > G, rs208265410 in the WNK1 gene was detected from the database of bovine genomic variation (BGVD). Here, we collected 328 individuals of 17 breeds representing four groups of Chinese cattle, northern group cattle, southern group cattle, central group cattle, and special group cattle (Tibetan cattle). We also collected the temperature and humidity data records from their relative locations. The frequencies of the G allele in Chinese breeds increased from northern China to southern China, and the frequencies of the A allele showed an opposite trend. Our results indicate that the WNK1 gene might be a candidate gene marker associated with cold tolerance.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"4803-4808"},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9384515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic association using principal components of morphometric traits in horses: identification of genes related to bone growth. 利用马的形态特征主成分进行基因组关联：鉴定与骨骼生长有关的基因。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-05-15 DOI: 10.1080/10495398.2023.2209795

Marisa Silva Bastos, Iara Del Pilar Solar Diaz, Jackeline Santos Alves, Louise Sarmento Martins de Oliveira, Chiara Albano de Araújo de Oliveira, Fernanda Nascimento de Godói, Gregório Miguel Ferreira de Camargo, Raphael Bermal Costa

{"title":"Genomic association using principal components of morphometric traits in horses: identification of genes related to bone growth.","authors":"Marisa Silva Bastos, Iara Del Pilar Solar Diaz, Jackeline Santos Alves, Louise Sarmento Martins de Oliveira, Chiara Albano de Araújo de Oliveira, Fernanda Nascimento de Godói, Gregório Miguel Ferreira de Camargo, Raphael Bermal Costa","doi":"10.1080/10495398.2023.2209795","DOIUrl":"10.1080/10495398.2023.2209795","url":null,"abstract":"The measurement of morphometric traits in horses is important for determining breed qualification and is one of the main selection criteria for the species. The development of an index (HPC) that consists of principal components weighted by additive genetic values allows to explore the most relevant relationships using a reduced number of variables that explain the greatest amount of variation in the data. Genome-wide association studies (GWAS) using HPC are a relatively new approach that permits to identify regions related to a set of traits. The aim of this study was to perform GWAS using HPC for 15 linear measurements as the explanatory variable in order to identify associated genomic regions and to elucidate the biological mechanisms linked to this index in Campolina horses. For GWAS, weighted single-step GBLUP was applied to HPC. The eight genomic windows that explained the highest proportion of additive genetic variance were identified. The sum of the additive variance explained by the eight windows was 95.89%. Genes involved in bone and cartilage development were identified (SPRY2, COL9A2, MIR30C, HEYL, BMP8B, LTBP1, FAM98A, and CRIM1). They represent potential positional candidates for the HPC of the linear measurements evaluated. The HPC is an efficient alternative to reduce the 15 usually measured traits in Campolina horses. Moreover, candidate genes inserted in region that explained high additive variance of the HPC were identified and might be fine-mapped for searching putative mutation/markers.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"4921-4926"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tryptophan alleviates intestinal damage through regulating necroptosis and TLR4/NOD signaling pathways in pigs after lipopolysaccharide challenge. 色氨酸通过调节猪在脂多糖挑战后的坏死和TLR4/NOD信号通路，减轻肠道损伤。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2023-05-18 DOI: 10.1080/10495398.2023.2213278

Jingyuan Tao, Guangmang Liu, Ke Gu, Gang Jia, Hua Zhao, Xiaoling Chen, Gang Tian, Jingyi Cai, Jing Wang

{"title":"Tryptophan alleviates intestinal damage through regulating necroptosis and TLR4/NOD signaling pathways in pigs after lipopolysaccharide challenge.","authors":"Jingyuan Tao, Guangmang Liu, Ke Gu, Gang Jia, Hua Zhao, Xiaoling Chen, Gang Tian, Jingyi Cai, Jing Wang","doi":"10.1080/10495398.2023.2213278","DOIUrl":"10.1080/10495398.2023.2213278","url":null,"abstract":"This study aimed to test the hypothesis that necroptosis, toll-like receptor 4 (TLR4)/nucleotide-binding oligomerization domain (NOD) signaling pathway in the jejunum of lipopolysaccharide (LPS)-challenged piglets are involved in the alleviation of intestinal injury and inflammation by tryptophan supplementation. Tryptophan supplementation has improved intestinal morphology. Also, tryptophan has been found to increase the mRNA and protein expression of tight junction proteins and decrease the expression of pro-inflammatory cytokines. Dietary tryptophan decreased the mRNA expression of heat shock protein 70, TLR4, NOD1, NOD2, myeloid differentiation primary response gene 88, interleukin 1 receptor-associated kinase 1, TNF receptor-associated factor 6, receptor-interacting serine/threonine-protein kinase 2-like, nuclear factor-kappaB transcription factor P65 in the jejunum of piglets. Tryptophan alleviated LPS-induced necroptosis and decreased the mRNA expression of mixed lineage kinase domain-like, receptor-interacting serine/threonine kinase 1, receptor-interacting serine/threonine-protein kinase 3-like, Fas (TNFRSF6)-associated via death domain, PGAM family member 5. Collectively, our results suggest that tryptophan supplementation helps in the attenuation of intestinal injury and inflammation by alleviating necroptosis and TLR4/NOD in lipopolysaccharide-challenged pigs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"4938-4946"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9484548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages. 不同繁殖阶段雌羊结肠旁和下丘脑转录组的特征。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2022-12-19 DOI: 10.1080/10495398.2022.2155174

Kenza Lakhssassi, Irene Ureña, Belén Marín, María Pilar Sarto, Belén Lahoz, José Luis Alabart, Jorge Hugo Calvo, Malena Serrano

{"title":"Characterization of the pars tuberalis and hypothalamus transcriptome in female sheep under different reproductive stages.","authors":"Kenza Lakhssassi, Irene Ureña, Belén Marín, María Pilar Sarto, Belén Lahoz, José Luis Alabart, Jorge Hugo Calvo, Malena Serrano","doi":"10.1080/10495398.2022.2155174","DOIUrl":"10.1080/10495398.2022.2155174","url":null,"abstract":"For understanding the molecular events underlying the follicular (F) and luteal (L) phases of estrous cycle, and anestrous (A) phase, the pars tuberalis (PT), and hypothalamus (HT) transcriptomes of 21 ewes were studied. In HT, 72 and 3 differential expression genes (DEGs) were found when comparing F vs. A and L vs. A, respectively. In PT, 6 and 4 DEGs were found in F vs. A and L vs. A comparisons, respectively. Enrichment analysis for DEGs between the F and A phases in the HT revealed significant clusters, mainly associated with actin-binding, and cytoskeleton, that are related to neural plasticity modulated by gonadal steroid hormones, as well as with oxytocin signaling. We found that DEGs in PT had higher differences in expression levels than those found in HT. In this sense, the ITLN was highly upregulated in the F and L vs. A phases, being MRPL57 and IRX4 highly downregulated in L vs. A comparison. The DDC gene in PT, related to LH regulation, was upregulated in the F phase. The gene set enrichment analysis (GSEA) revealed multiple pathways related to neurotransmission and neuronal plasticity. Our study reveals new candidate genes involved in the reproductive stages' transitions in seasonal sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"3461-3474"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10442420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of sugarcane bagasse on in vitro degradability, rumen characteristics, nutrients digestibility, blood parameters and milk production of lactating buffaloes. 甘蔗渣对泌乳水牛体外降解性、瘤胃特征、营养物质消化率、血液参数和产奶量的影响

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2022-12-19 DOI: 10.1080/10495398.2022.2149546

Abdel-Alim Abd El-Mola, Shaaban S Elnesr

{"title":"Influence of sugarcane bagasse on in vitro degradability, rumen characteristics, nutrients digestibility, blood parameters and milk production of lactating buffaloes.","authors":"Abdel-Alim Abd El-Mola, Shaaban S Elnesr","doi":"10.1080/10495398.2022.2149546","DOIUrl":"10.1080/10495398.2022.2149546","url":null,"abstract":"This study aimed to evaluate the use of sugarcane bagasse (SCB) as exclusive roughage in lactating buffaloes on digestibility, milk production and composition, and microbial protein. In vitro dry matter digestion (IVDMD) and organic matter digestion (IVOMD) for SCB as a replacement for barley straw (BS) of the control ration have been determined. In vivo experiment, 55 lactating buffaloes were randomly assigned into five groups. First group was fed the control ration (60% concentrate feed mixture (CFM) and 40% BS), second group was fed 60% CFM and 30% BS + 10% SCB, third group was fed 60% CFM and 20% BS + 20% SCB, fourth group was fed 60% CFM and 10% BS + 30% SCB and fifth group was fed 60% CFM and 40% SCB. Results indicated that IVDMD% and IVOMD% degradability were increased with the inclusion SCB in rations compared with the control. Full replacement of BS by SCB 40% significantly (p < 0.05) increased nutrients digestibility coefficient with improving ruminal basic parameters. Buffaloes fed SCB40 had higher milk component yields, 4% fat corrected milk and plasma proteins, and lower plasma creatinine and cholesterol than control buffaloes (p < 0.05). Finally, the inclusion of SCB up to 40% in lactating buffaloes rations favorably affected rumen fermentation characteristics (in vitro) and improved nutrients digestibility and milk production (in vivo).","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"3378-3386"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10442423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activated macrophages of CD 163 gene edited pigs generated by direct cytoplasmic microinjection with CRISPR gRNA/Cas9 mRNA are resistant to PRRS virus assault. 通过细胞质直接显微注射CRISPR gRNA/Cas9 mRNA产生的cd163基因编辑猪活化巨噬细胞可抵抗PRRS病毒的攻击。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2022-05-04 DOI: 10.1080/10495398.2022.2062602

Shao-Wen Hung, Chin-Kai Chuang, Chi-Hong Wong, Chon-Ho Yen, Shu-Hui Peng, Chieh Yang, Ming-Cheng Chen, Tien-Shuh Yang, Ching-Fu Tu

{"title":"Activated macrophages of CD 163 gene edited pigs generated by direct cytoplasmic microinjection with CRISPR gRNA/Cas9 mRNA are resistant to PRRS virus assault.","authors":"Shao-Wen Hung, Chin-Kai Chuang, Chi-Hong Wong, Chon-Ho Yen, Shu-Hui Peng, Chieh Yang, Ming-Cheng Chen, Tien-Shuh Yang, Ching-Fu Tu","doi":"10.1080/10495398.2022.2062602","DOIUrl":"10.1080/10495398.2022.2062602","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) infects placental and lung macrophages, causing a global epidemic with economic loss. Attempts to develop an effective vaccine to control the disease have not been effective. Currently, developing PRRSV disease-resistant pigs via a gene editing (GE) strategy to mutate the PRRSV receptor or to delete the binding domain on the macrophage appears promising. In this study, we used the strategy of Edinburg University to construct two guide RNAs (gRNAs) located on the proximal front and post sites of exon 7. Directive microinjection of two gRNAs and Cas9 mRNA into the cytoplasm of pronuclear zygotes efficiently generated four piglets confirmed as CD163 knockout (KO) and/or CD163 exon 7 deleted (CD163ΔE7). In four GE piglets, three pigs carried two chromosome CD163 KO or ΔE7. Peripheral blood mononuclear cells (PBMCs) from three GE and wild-type (WT) pigs were activated into macrophages for in vitro transfection. The results showed that the activated macrophages from all GE pigs were significantly more viable than those from WT pig. Current results suggest that we have successfully generated PRRSV-resistant pigs, although in vivo challenge is needed to validate that the pigs are PRRSV resistant.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"1 1","pages":"4196-4209"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46713355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of mitochondrial 12S rRNA gene of yellow-striped chevrotain (Moschiola kathygre) and white-spotted chevrotain (Moschiola meminna) and development of a PCR-RFLP marker for the unambiguous identification of the species. 黄条纹人轮蛋白（Moschiola kathygre）和白斑人轮蛋白的线粒体12S rRNA基因的鉴定以及用于明确鉴定该物种的PCR-RFLP标记的开发。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2022-05-03 DOI: 10.1080/10495398.2022.2070183

Tharanya Sugumar, Danushka S Weerasekera, Kithsiri B Ranawana, Nadeeka U Jayawardana

{"title":"Characterization of mitochondrial 12S rRNA gene of yellow-striped chevrotain (Moschiola kathygre) and white-spotted chevrotain (Moschiola meminna) and development of a PCR-RFLP marker for the unambiguous identification of the species.","authors":"Tharanya Sugumar, Danushka S Weerasekera, Kithsiri B Ranawana, Nadeeka U Jayawardana","doi":"10.1080/10495398.2022.2070183","DOIUrl":"10.1080/10495398.2022.2070183","url":null,"abstract":"Tragulids hold a significant place in the evolutionary history of mammals since they represent the basal branch of ruminants. Only three genera of tragulids are being extant to date such as Tragulus, Hyemoschus and Moschiola. In the genus Moschiola, Sri Lankan chevrotains (Moschiola meminna and Moschiola kathygre) are endemic to Sri Lanka while Moschiola indica is present in India. Sri Lankan chevrotains lack information on their population structure, distribution and molecular evidence on species identification. This leads to possible threats including habitat destruction, poaching and illegal hunting and trading under different names. In this study, genomic DNA from hair follicles was isolated from M. meminna and M. kathygre and 12S rRNA mitochondrial gene was amplified using universal primers. The PCR products were sequenced and phylogenetic analysis was done on Sri Lankan chevrotains for the first time. The sequences of Sri Lankan chevrotains share 99.7% similarity as they differ only in a single InDel present in M. meminna. In silico analysis of 12S rRNA region revealed that PCR-RFLP approach can be used to differentiate the Sri Lankan chevrotains from Indian chevrotain using the restriction enzymes; Rsa I, Sca I, Hinf I and Hind III. M. kathygre and M. meminna can be differentiated from each other by using Dra I.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"34 7","pages":"2065-2072"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41098217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of storage duration and short periods of incubation during egg storage on embryonic development and hatching traits of hybrid chicken strain. 鸡蛋贮藏期间贮藏时间和短孵化期对杂交鸡胚胎发育和孵化特性的影响。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2023-12-01 Epub Date: 2024-01-30 DOI: 10.1080/10495398.2023.2260840

Samuel Addo Ansah, Edna Mariam Ackah, Michael Boateng, Latifah Nurudeen, Fredrick Nyarko, Kwaku Adusei Acheampong, Prince Sasu, Jacob Alhassan Hamidu

{"title":"Impact of storage duration and short periods of incubation during egg storage on embryonic development and hatching traits of hybrid chicken strain.","authors":"Samuel Addo Ansah, Edna Mariam Ackah, Michael Boateng, Latifah Nurudeen, Fredrick Nyarko, Kwaku Adusei Acheampong, Prince Sasu, Jacob Alhassan Hamidu","doi":"10.1080/10495398.2023.2260840","DOIUrl":"10.1080/10495398.2023.2260840","url":null,"abstract":"The study assessed chicken egg storage duration and short periods of incubation during egg storage (SPIDES) on embryo development. Eggs samples from 310 eggs from 72-week-old hybrid layer breeder flocks were divided and stored at 16 °C and 75% RH for 1, 4, 7, 10, 14, 17, and 21 days (D). Some eggs were also divided between 14D and 21D alone and stored. One subgroup received heat application for 6 h during storage resulting in 0-h × 14D (control); 0-h × 21D (control); 6-h × 14D (SPIDES) and 6-h × 21D (SPIDES). Storage durations between 1D and 21D did not influence most egg quality parameters. The interaction of storage duration and SPIDES affected initial, final egg weights and egg weight loss. SPIDES eggs recorded heavier wet embryos than non-SPIDES eggs at embryonic days (ED) 4, ED7, ED11, and dry embryo weight at ED11. SPIDES resulted in longer embryos at ED4 and ED11. In 21D stored eggs hatchability was higher in SPIDES compared to non-SPIDES treatments (66.7 vs. 48.3%). The reverse effect of SPIDES occurred in 14D stored eggs. In conclusion, the study shows positive effect of SPIDES in longer stored eggs and could be due to a positive impact on embryo.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"4081-4093"},"PeriodicalIF":3.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41109097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0