Animal Biotechnology最新文献_第10页

Weighted single step GWAS reveals genomic regions associated with economic traits in Murrah buffaloes. 加权单步 GWAS 发现了与 Murrah 水牛经济性状相关的基因组区域。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-03-04 DOI: 10.1080/10495398.2024.2319622

Linda George, Rani Alex, Gopal Gowane, Vikas Vohra, Pooja Joshi, Ravi Kumar, Archana Verma

{"title":"Weighted single step GWAS reveals genomic regions associated with economic traits in Murrah buffaloes.","authors":"Linda George, Rani Alex, Gopal Gowane, Vikas Vohra, Pooja Joshi, Ravi Kumar, Archana Verma","doi":"10.1080/10495398.2024.2319622","DOIUrl":"10.1080/10495398.2024.2319622","url":null,"abstract":"The objective of the present study was to identify genomic regions influencing economic traits in Murrah buffaloes using weighted single step Genome Wide Association Analysis (WssGWAS). Data on 2000 animals, out of which 120 were genotyped using a double digest Restriction site Associated DNA (ddRAD) sequencing approach. The phenotypic data were collected from NDRI, India, on growth traits, viz., body weight at 6M (month), 12M, 18M and 24M, production traits like 305D (day) milk yield, lactation length (LL) and dry period (DP) and reproduction traits like age at first calving (AFC), calving interval (CI) and first service period (FSP). The biallelic genotypic data consisted of 49353 markers post-quality check. The heritability estimates were moderate to high, low to moderate, low for growth, production, reproduction traits, respectively. Important genomic regions explaining more than 0.5% of the total additive genetic variance explained by 30 adjacent SNPs were selected for further analysis of candidate genes. In this study, 105 genomic regions were associated with growth, 35 genomic regions with production and 42 window regions with reproduction traits. Different candidate genes were identified in these genomic regions, of which important are OSBPL8, NAP1L1 for growth, CNTNAP2 for production and ILDR2, TADA1 and POGK for reproduction traits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2319622"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140020805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copy number variations within fibroblast growth factor 13 gene influence growth traits and alternative splicing in cattle. 成纤维细胞生长因子 13 基因拷贝数变异对牛生长特性和替代剪接的影响

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-03-01 DOI: 10.1080/10495398.2024.2314104

Hanfang Cai, Xin Li, Xinran Niu, Jing Li, Xianyong Lan, Chuzhao Lei, Yongzhen Huang, Huifen Xu, Ming Li, Hong Chen

{"title":"Copy number variations within fibroblast growth factor 13 gene influence growth traits and alternative splicing in cattle.","authors":"Hanfang Cai, Xin Li, Xinran Niu, Jing Li, Xianyong Lan, Chuzhao Lei, Yongzhen Huang, Huifen Xu, Ming Li, Hong Chen","doi":"10.1080/10495398.2024.2314104","DOIUrl":"10.1080/10495398.2024.2314104","url":null,"abstract":"Previous researches revealed a copy number variation (CNV) region in the bovine fibroblast growth factor 13 (FGF13) gene. However, its effects remain unknown. This study detected the various copy number types in seven Chinese cattle breeds and analysed their population genetic characteristics and effects on growth traits and transcription levels. Copy number Loss was more frequent in Caoyuan Red cattle and Xianan cattle than in the other breeds. Association analysis between CNV and growth traits of Qinchuan indicated that the CNV was significantly related to chest depth, hip width and hucklebone width (P < 0.05). Additionally, the growth traits of individuals with copy number Loss were significantly inferior to those with copy number Gain or Median (P < 0.05). Besides, we found two splicing isoforms, AS1 and AS2, in FGF13 gene, which resulted from alternative 5' splicing sites of intron 1. These isoforms showed varied expression levels in various tissues. Moreover, CNV was significantly and negatively associated with the mRNA expression of AS1 (r = -0.525, P < 0.05). The CNVs in bovine FGF13 gene negatively regulated growth traits and gene transcription. These observations provide new insights into bovine FGF13 gene, delivering potentially useful information for future Chinese cattle breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2314104"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of miR-361-5p promotes the induced activation of SHF-stem cells through FOXM1 mediated Wnt/β-catenin pathway in cashmere goats. 敲除 miR-361-5p 可通过 FOXM1 介导的 Wnt/β-catenin 通路促进羊绒山羊 SHF 干细胞的诱导活化。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-28 DOI: 10.1080/10495398.2024.2356110

Ruqing Xu, Man Bai, Yixing Fan, Yubo Zhu, Zeying Wang, Taiyu Hui, Qi Zhang, Xingwang Liu, Jialiang Zhang, Jincheng Shen, Wenlin Bai

{"title":"Knockdown of miR-361-5p promotes the induced activation of SHF-stem cells through FOXM1 mediated Wnt/β-catenin pathway in cashmere goats.","authors":"Ruqing Xu, Man Bai, Yixing Fan, Yubo Zhu, Zeying Wang, Taiyu Hui, Qi Zhang, Xingwang Liu, Jialiang Zhang, Jincheng Shen, Wenlin Bai","doi":"10.1080/10495398.2024.2356110","DOIUrl":"10.1080/10495398.2024.2356110","url":null,"abstract":"The inducing activation event of secondary hair follicle (SHF)-stem cells is considered a key biological process in the SHF regeneration, and the morphogenesis of cashmere fiber in cashmere goats. The miR-361-5p was essentially implicated in the induced activation of SHF-stem cells of cashmere goats, but its functional mechanisms are unclear. Here, we confirmed miR-361-5p was significantly downregulated in anagen SHF bugle of cashmere goats compared with that at telogen, and miR-361-5p expression was significantly lower in SHF-stem cells after activation than its counterpart before activation. Further, we found that miR-361-5p could negatively regulate the induced activation event of SHF-stem cells in cashmere goats. Mechanistically, through dual-luciferase reporter assays, miR-361-5p specifically bound with FOXM1 mRNA in SHF-stem cells of cashmere goats and negatively regulated the expression of FOXM1 gene. Also, through overexpression/knockdown analysis of FOXM1 gene, our results indicated that FOXM1 upregulated the expression of Wnt/β-catenin pathway related genes in SHF-stem cells. Moreover, based on TOP/FOP-flash Wnt report assays, the knockdown of miR-361-5p promotes the Wnt/β-catenin pathway activation through upregulating the FOXM1 expression in SHF-stem cells. Finally, we demonstrated that miR-361-5p negatively regulated the induced activation of SHF-stem cells through FOXM1 mediated Wnt/β-catenin pathway in cashmere goats.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2356110"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysing the genetic diversity of three sheep breeds in Turkey and nearby countries using 50 K SNPs data. 利用 50 K SNPs 数据分析土耳其及附近国家三个绵羊品种的遗传多样性。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-03-18 DOI: 10.1080/10495398.2024.2329106

Mervan Bayraktar

{"title":"Analysing the genetic diversity of three sheep breeds in Turkey and nearby countries using 50 K SNPs data.","authors":"Mervan Bayraktar","doi":"10.1080/10495398.2024.2329106","DOIUrl":"10.1080/10495398.2024.2329106","url":null,"abstract":"This study analysed the genetic diversity and population structure of eight sheep breeds in Turkey and nearby countries. Moderate genetic diversity was observed, with the Sakiz (SKZ) exhibiting the highest diversity based on heterozygosity and allelic richness (AR) values. Genetic distances revealed differentiation between the populations, with the most significant divergence between the Cyprus Fat Tail (CFT) and SKZ breeds. PCA demonstrated SKZ and Chios (CHI) clustering together, indicating genetic similarity. Karakas (KRS), Norduz (NDZ), Afshari (AFS), Moghani (MOG) and others showed overlap, reflecting genetic relationships. Ancestry analysis found that KRS was predominantly inherited from the second ancestral population, while SKZ and NDZ were primarily derived from the first and second ancestral lineages. This illustrated the populations' diverse origins. Most genetic variation (96.84%) was within, not between, populations. The phi-statistic (PhiPT) indicated moderate differentiation overall. Phylogenetic analysis further demonstrated the genetic distinctiveness of the SKZ breed. ROH and FROH analyses showed that SKZ exhibited the highest homozygosity and inbreeding, while KRS displayed the lowest. This study elucidates these breeds' genetic diversity, structure and relationships. Key findings include moderate diversity, evidence of differentiation between breeds, diverse ancestral origins and distinct ROH patterns. This provides insights into the population's genetic characteristics and conservation requirements.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2329106"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140142599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature adaptation patterns in Chinese cattle revealed by TRPM2 gene mutation analysis. 通过TRPM2基因突变分析揭示中国牛的温度适应模式

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-02 DOI: 10.1080/10495398.2023.2299944

Dekai Liu, Yifan Yang, Zhefu Chen, Yijie Fan, Jianyong Liu, Yibing Xu, Zulfiqar Ahmed, Jicai Zhang, Fuqiang Li, Xingshan Qi, Weiru Song, Kaixia Zhu, Jiangcai Gongque, Guomei Li, Bizhi Huang, Chuzhao Lei

{"title":"Temperature adaptation patterns in Chinese cattle revealed by TRPM2 gene mutation analysis.","authors":"Dekai Liu, Yifan Yang, Zhefu Chen, Yijie Fan, Jianyong Liu, Yibing Xu, Zulfiqar Ahmed, Jicai Zhang, Fuqiang Li, Xingshan Qi, Weiru Song, Kaixia Zhu, Jiangcai Gongque, Guomei Li, Bizhi Huang, Chuzhao Lei","doi":"10.1080/10495398.2023.2299944","DOIUrl":"10.1080/10495398.2023.2299944","url":null,"abstract":"Cattle are sensitive to temperature fluctuations but adapt well to inclement weather conditions. When environmental temperatures exceed specific thresholds, heat stress becomes a critical concern for cattle. The TRPM2 gene, which resides on cattle chromosome 1 encodes a TRP channel protein, holding a unique capacity to sense temperature changes and facilitate rapid response to avoid heat stress. Here, we utilized the Bovine Genome Variation Database (BGVD) (http://animal.omics.pro/code/index.php/BosVar), and identified a missense mutation site, c.805A > G: p. Met269Val (rs527146862), within the TRPM2 gene. To elucidate the functional assessment of this mutation in temperature adaptation attributes of Chinese cattle, we genotyped 407 samples from 20 distinct breeds representing diverse climatic zones across China. The association analysis incorporates three temperature parameters and revealed compelling insights in terms of allele frequency. Interestingly, the prevalence of the wild-type allele A was notably higher among northern cattle breeds and this trend diminished gradually as observed in southern cattle populations. Conversely, the mutant-type allele G demonstrated a contrasting trend. Moreover, southern cattle exhibited markedly higher frequencies of GG and GA genotypes (P < 0.01). The presence of heterozygous and homozygous mutations appears to confer an enhanced capacity for adaptation to elevated temperatures. These results provide unequivocal correlation evidence between TRPM2 genotypes (AA, GA, GG) and environmental temperature parameters and comprehend the genetic mechanisms governing temperature adaptation in cattle. This provides valuable insights for strategic breed selection across diverse climatic regions, thereby aiding livestock production amid evolving climate challenges.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2299944"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

InDel mutations within the bovine PER2 gene are significantly associated with reproductive traits. 牛 PER2 基因中的 InDel 突变与繁殖性状有显著相关性。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI: 10.1080/10495398.2024.2397806

Yuanzhe Yang, Jiajun Song, Siyuan Shen, Yongsheng Wang, Chuanying Pan, Jiyao Wu, Xianyong Lan

{"title":"InDel mutations within the bovine PER2 gene are significantly associated with reproductive traits.","authors":"Yuanzhe Yang, Jiajun Song, Siyuan Shen, Yongsheng Wang, Chuanying Pan, Jiyao Wu, Xianyong Lan","doi":"10.1080/10495398.2024.2397806","DOIUrl":"10.1080/10495398.2024.2397806","url":null,"abstract":"Functioning as a key regulator of circadian rhythms, the PER2 gene exerts a substantial impact on the reproductive traits of animals. However, the effect of the PER2 gene on ovarian development remains unclear. In order to examine the relationship between bovine reproductive trait and the PER2 gene, a total of 901 ovarian samples were collected, categorized into different oestrus cycles (proestrus, oestrus, post-oestrus, anoestrous), and subjected to analysis for two potential insertion/deletions (InDels) in the PER2 gene. Through agarose gel electrophoresis and DNA sequencing, two polymorphic deletion mutations (P2-D5-bp, P3-D13-bp) were identified. Furthermore, a significant association between mature follicle diameter and P2-D5-bp was found (P < 0.05). Additionally, several significant correlations with ovarian length, width, height, and white body diameter were found for P3-D13-bp (P < 0.05). These findings suggested that the bovine PER2 gene plays an important role in above-mentioned reproductive traits, offering new avenues for improving cow fertility through marker-assisted selection (MAS).","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2397806"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of supplemental Glycyrrhiza polysaccharide on growth performance and intestinal health in weaned piglets. 补充甘草多糖对断奶仔猪生长性能和肠道健康的影响

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-06-11 DOI: 10.1080/10495398.2024.2362640

Xueying Wang, Pengli Zhao, Cai Zhang, Chenxu Li, Yanbo Ma, Shucheng Huang

{"title":"Effects of supplemental Glycyrrhiza polysaccharide on growth performance and intestinal health in weaned piglets.","authors":"Xueying Wang, Pengli Zhao, Cai Zhang, Chenxu Li, Yanbo Ma, Shucheng Huang","doi":"10.1080/10495398.2024.2362640","DOIUrl":"10.1080/10495398.2024.2362640","url":null,"abstract":"In this study, we investigated the effects of supplemental Glycyrrhiza polysaccharide (GCP) on growth performance and intestinal health of weaned piglets. Ninety piglets weaned at 28 days of age were randomly allocated to three groups with five replicates per treatment. Piglets were fed the following diets for 28 days: (1) CON (control group), basal diet; (2) G500, CON + 500 mg/kg GCP; (3) G1000, CON + 1000 mg/kg GCP. The results showed that supplementation with 1000 mg/kg GCP increased the average daily gain (ADG) and decreased the feed-to-gain ratio (F/G) (P < 0.05). Serum diamine oxidase (DAO) and D-lactic acid (DL-A) levels were lower in the G1000 group (P < 0.05). Dietary GCP 1000 mg/kg improved mucosal trypsin activity in the duodenum, jejunum and ileum and increased lipase and amylase activity in the jejunum (P < 0.05). Moreover, in the G1000 group, ZO-1, claudin 1 and occludin levels were increased in the jejunum mucosa, whereas interleukin-1β (IL-1β) and IL-6 levels were decreased (P < 0.05). The 16S rRNA gene analysis indicated that dietary 1000 mg/kg GCP altered the jejunal microbial community, with increased relative abundances of beneficial bacteria. In conclusion, dietary GCP 1000 mg/kg can improve growth performance, digestive enzyme activity, intestinal immunity, barrier function and microbial community in weaned piglets.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2362640"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory properties of Bacillus pumilus TS1 in lipopolysaccharide-induced inflammatory damage in broilers. 鼠疫杆菌 TS1 在脂多糖诱发肉鸡炎症损伤中的抗炎特性

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-10-25 DOI: 10.1080/10495398.2024.2418516

Yinkun Liu, Sirui Liu, Shuangshuang Wan, Zixin Li, Hao Li, Shu Tang

{"title":"Anti-inflammatory properties of Bacillus pumilus TS1 in lipopolysaccharide-induced inflammatory damage in broilers.","authors":"Yinkun Liu, Sirui Liu, Shuangshuang Wan, Zixin Li, Hao Li, Shu Tang","doi":"10.1080/10495398.2024.2418516","DOIUrl":"10.1080/10495398.2024.2418516","url":null,"abstract":"This study investigates whether Bacillus pumilus TS1 improves growth performance and alleviates inflammatory damage in broilers and explored its feasibility as an antibiotic alternative. We divided 240 one-day-old AA308 white-finned broilers into five groups (con, LPS, TS1L + LPS, TS1M + LPS and TS1H + LPS). The TS1L + LPS, TS1M + LPS and TS1H + LPS groups were fed TS1 for 15 days by gavage. The LPS, TS1L + LPS, TS1M + LPS and TS1H + LPS groups were injected intraperitoneally with 1 mg/kg LPS for three days. We investigated the probiotic and anti-inflammatory activities by measuring body weight, sequencing the intestinal flora and examining the structure of tissues by using pathological stain, real-time PCR, Western blotting and immunohistochemical detection. TS1 could improve growth performance and intestinal flora composition, also reduced different organ damage and inflammatory cytokine expression in serum and organs. The mechanism may involve upregulating HSP60 and HSP70 expression, targeting and regulating Nrf2 and P38 MAPK and modulating NF-κB and HO-1 expression at the transcriptional level in different organs. B. pumilus TS1 alleviated Inflammatory injury caused by LPS and attenuated the inflammatory response in broilers, and these effects were achieved through MAPK and Nrf2 regulation of HSPs/HO-1 in different organs. The above results suggested broilers fed with TS1 could release the LPS caused organ damage, and the most suggested dosage was 1.4 × 108 CFU/mL.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2418516"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide. 对脂多糖诱导的牛乳腺上皮细胞进行转录组测序分析。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-23 DOI: 10.1080/10495398.2023.2290527

Jingjing Liu, Yingkui Gao, Xing Zhang, Zhonghua Hao, Huaqiang Zhang, Rong Gui, Fang Liu, Chao Tong, Xuebing Wang

{"title":"Transcriptome sequencing analysis of bovine mammary epithelial cells induced by lipopolysaccharide.","authors":"Jingjing Liu, Yingkui Gao, Xing Zhang, Zhonghua Hao, Huaqiang Zhang, Rong Gui, Fang Liu, Chao Tong, Xuebing Wang","doi":"10.1080/10495398.2023.2290527","DOIUrl":"10.1080/10495398.2023.2290527","url":null,"abstract":"Mastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic bacteria, playing an essential role in immune defense. To investigate the mechanism of bovine mammary epithelial cells in the inflammatory process, we treated the cells with LPS for 12 hours and analyzed the changes in mRNA by transcriptome sequencing. The results showed that compared to the control group, the LPS treatment group had 121 up-regulated genes and 18 down-regulated genes. GO and KEGG enrichment analysis revealed that these differential genes were mainly enriched in the IL-17 signaling pathway, Legionellosis, Cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, and other signaling pathways. Furthermore, the expression of GRO1 and CXCL3 mRNAs increased significantly after LPS treatment. These findings provide new insights for the treatment of mastitis in cows in the future.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2290527"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138883895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PI3K/AKT/mTORC1 signalling pathway regulates MMP9 gene activation via transcription factor NF-κB in mammary epithelial cells of dairy cows. PI3K/AKT/mTORC1 信号通路通过转录因子 NF-κB 调节奶牛乳腺上皮细胞中 MMP9 基因的激活。

IF 1.8 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-02-12 DOI: 10.1080/10495398.2024.2314100

Yongjin Mao, Chen Su, Huilin Yang, Xiaocong Ma, Feng Zhao, Bo Qu, Yang Yang, Xiaoming Hou, Bing Zhao, Yingjun Cui

{"title":"PI3K/AKT/mTORC1 signalling pathway regulates MMP9 gene activation via transcription factor NF-κB in mammary epithelial cells of dairy cows.","authors":"Yongjin Mao, Chen Su, Huilin Yang, Xiaocong Ma, Feng Zhao, Bo Qu, Yang Yang, Xiaoming Hou, Bing Zhao, Yingjun Cui","doi":"10.1080/10495398.2024.2314100","DOIUrl":"10.1080/10495398.2024.2314100","url":null,"abstract":"Matrix metalloproteinase 9 (MMP9) plays a pivotal role in mammary ductal morphogenesis, angiogenesis and glandular tissue architecture remodeling. However, the molecular mechanism of MMP9 expression in mammary epithelial cells of dairy cows remains unclear. This study aimed to explore the underlying mechanism of MMP9 expression. In this study, to determine whether the PI3K/AKT/mTORC1/NF-κB signalling pathway participates in the regulation of MMP9 expression, we treated mammary epithelial cells with specific pharmacological inhibitors of PI3K (LY294002), mTORC1 (Rapamycin) or NF-κB (Celastrol), respectively. Western blotting results indicated that LY294002, Rapamycin and Celastrol markedly decreased MMP9 expression and P65 nuclear translocation. Furthermore, we found that NF-κB (P65) overexpression resulted in elevated expression of MMP9 protein and activation of MMP9 promoter. In addition, we observed that Celastrol markedly decreases P65-overexpression-induced MMP9 promoter activity. Moreover, the results of the promoter assay indicated that the core regulation sequence for MMP9 promoter activation may be located at -420 ∼ -80 bp downstream from the transcription start site. These observations indicated that the PI3K/AKT/mTORC1 signalling pathway is involved in MMP9 expression by regulating MMP9 promoter activity via NF-κB in the mammary epithelial cells of dairy cows.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2314100"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139721247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0