Animal Biotechnology最新文献_第10页

Discover QTLs for the level of blood components in Shaoxing duck using GWAS and haplotype sharing analysis. 利用 GWAS 和单倍型共享分析发现绍兴鸭血液成分水平的 QTLs。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-08-13 DOI: 10.1080/10495398.2024.2390940

Wenwu Xu, Zhenzhen Wang, Zeng Tao, Kui Li, Lizhi Lu

{"title":"Discover QTLs for the level of blood components in Shaoxing duck using GWAS and haplotype sharing analysis.","authors":"Wenwu Xu, Zhenzhen Wang, Zeng Tao, Kui Li, Lizhi Lu","doi":"10.1080/10495398.2024.2390940","DOIUrl":"https://doi.org/10.1080/10495398.2024.2390940","url":null,"abstract":"Blood composition is indicative of health-related traits such as immunity and metabolism. The use of molecular genetics to investigate alterations in these attributes in laying ducks is a novel approach. Our objective was to employ genome - wide association studies (GWAS) and haplotype - sharing analysis to identify genomic regions and potential genes associated with 11 blood components in Shaoxing ducks. Our findings revealed 35 SNPs and 1 SNP associated with low-density lipoprotein cholesterol (LDL) and globulin (GLB), respectively. We identified 36 putative candidate genes for the LDL trait in close proximity to major QTLs and key loci. Based on their biochemical and physiological properties, TRA2A, NPY, ARHGEF26, DHX36, and AADAC are the strongest putative candidate genes. Through linkage disequilibrium analysis and haplotype sharing analysis, we identified three haplotypes and one haplotype, respectively, that were significantly linked with LDL and GLB. These haplotypes could be selected as potential candidate haplotypes for molecular breeding of Shaoxing ducks. Additionally, we utilized a bootstrap test to verify the reliability of GWAS with small experimental samples. The test can be accessed at https://github.com/xuwenwu24/Bootstrap-test.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2390940"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down-regulation of miR-29 improves lipid metabolism in fatty liver of dairy cows. 下调 miR-29 可改善奶牛脂肪肝的脂质代谢。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-08-29 DOI: 10.1080/10495398.2024.2396414

Xueying Wang, Cai Zhang, Rishun Li, Yan Qiu, Yanbo Ma, Shuai Wang, Yuanxiao Li, Shuai Guo, Chenxu Li

{"title":"Down-regulation of miR-29 improves lipid metabolism in fatty liver of dairy cows.","authors":"Xueying Wang, Cai Zhang, Rishun Li, Yan Qiu, Yanbo Ma, Shuai Wang, Yuanxiao Li, Shuai Guo, Chenxu Li","doi":"10.1080/10495398.2024.2396414","DOIUrl":"10.1080/10495398.2024.2396414","url":null,"abstract":"In this study, we conducted a thorough investigation into the mechanisms by which miR-29 influences lipid metabolism. Thirty-two cows were selected and categorized into distinct groups based on their liver triglyceride (TG) content: healthy, mild fatty liver, and moderate fatty liver groups. Dairy cows with moderate fatty liver showed higher levels of hepatic lipid accumulation, MDA content and serum AST, ALT and ALP contents and lower hepatic catalase CAT and SOD activities. Subsequently, hepatocytes isolated from healthy calves were exposed to sodium oleate (SO) in the presence or absence of pre-incubation with miR-29 inhibitor or inhibitor NC. Pre-transfection with miR-29 inhibitor resulted in reduced hepatocyte lipid accumulation and MDA levels, as well as decreased levels of AST, ALT, and ALP in the supernatant. In the miR-29 inhibitor + SO group, there was an increase in the expression of SREBP-1, FAS, SCD1, and Sirt1. Meanwhile, the expression of PPARα, CPT1, CPT2, PGC-1α, NRF-1, UCP2, and miR-29 were observed to be decreased. In comparison to the miR-29 inhibitor + SO group, some of the measured indicators showed partial reversal in the miR-29 inhibitor + siSirt1 + SO group. Collectively, these findings provide evidence that miR-29 may play a crucial role in the pathogenesis of fatty liver in dairy cows.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2396414"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle. 通过对汉宇牛背阔肌和腰大肌的转录组进行比较分析，揭示其分子特征多样性。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-07-25 DOI: 10.1080/10495398.2024.2379883

Sunirmal Sheet, Sun Sik Jang, Jin-A Lim, Woncheoul Park, Dahye Kim

{"title":"Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle.","authors":"Sunirmal Sheet, Sun Sik Jang, Jin-A Lim, Woncheoul Park, Dahye Kim","doi":"10.1080/10495398.2024.2379883","DOIUrl":"10.1080/10495398.2024.2379883","url":null,"abstract":"This study investigates the transcriptome-level alterations that influence production traits and early fattening stage myogenesis in Hanwoo cattle, specifically focusing on the highly prized Longissimus dorsi (LD) and Psoas major (PM) skeletal muscles, which hold significant commercial value. We conducted RNA sequencing analysis on LD and PM muscles from 14 Hanwoo steers (n = 7, each group) at the age of 10 months, all fed the same diet. Our results unveiled a total of 374 and 206 up-regulated differentially expressed genes (DEGs) in LD and PM muscles, respectively, with statistical significance (p < 0.05) and a log2fold change ≥ 1. Genes governing muscle development processes, such as PAX3, MYL3, COL11A1, and MYL6B, were found to be expressed at higher levels in both tissues. Conversely, genes regulating lipid metabolism, including FABP3, FABP4, LEP, ADIPOQ, and PLIN1, exhibited higher expression in the PM muscle. Functional enrichment analysis revealed a tissue-specific response, as PM muscle showed increased lipid metabolism allied pathways, including the PPAR signaling pathway and regulation of lipolysis in adipocytes, while LD was characterized by growth and proliferative processes. Our findings validate the presence of a muscle-dependent transcription and co-expression pattern that elucidates the transcriptional landscape of bovine skeletal muscle.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2379883"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep. 初步调查揭示了妊娠印度绵羊内分泌腺感染蓝舌病毒-1的新病理后果。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-10-18 DOI: 10.1080/10495398.2023.2269428

Rohit Singh, Karam Pal Singh, Rajendra Singh, Vidya Singh, Pawan Kumar, Rajat Varshney, Akanksha Yadav, Akash Mote, Mukesh Gangwar, N Babu Prasath

{"title":"Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep.","authors":"Rohit Singh, Karam Pal Singh, Rajendra Singh, Vidya Singh, Pawan Kumar, Rajat Varshney, Akanksha Yadav, Akash Mote, Mukesh Gangwar, N Babu Prasath","doi":"10.1080/10495398.2023.2269428","DOIUrl":"10.1080/10495398.2023.2269428","url":null,"abstract":"Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7th days postinoculation (DPI) and in thyroid and pituitary glands at 15th DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2269428"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41231836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional analysis of the GPAT4 gene mutation predicted to affect splicing. 预测影响剪接的GPAT4基因突变的功能分析。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-10-31 DOI: 10.1080/10495398.2023.2269210

Sergey A Bursakov, Anastasia V Kovaleva, Artyom V Brigida, Oleg G Zaripov

{"title":"Functional analysis of the GPAT4 gene mutation predicted to affect splicing.","authors":"Sergey A Bursakov, Anastasia V Kovaleva, Artyom V Brigida, Oleg G Zaripov","doi":"10.1080/10495398.2023.2269210","DOIUrl":"10.1080/10495398.2023.2269210","url":null,"abstract":"The GPAT4 gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the GPAT4 gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence variations (rs442541537), transfection experiments in a wild type and mutant cell line model system demonstrated that the investigated mutation in the second intron of the GPAT4 gene was responsible for the presence of a second exon in mature messenger RNA (mRNA). The cases of its absence in the spliced mature mRNA transcript resulted in a truncated dysfunctional protein due to the appearance of a stop codon. Thus, the discovered SNP led to alternative splicing in pre-mRNA by the 'cassette exon' ('exon skipping') mechanism. The studied mutation can potentially be a molecular genetic marker for alternative splicing for the GPAT4 gene and, therefore contributes to economic benefits in cattle breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2269210"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71419925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size. 根据卵泡大小，双相体外成熟（CAPA-IVM）对青春期前山羊卵母细胞的表皮生长因子受体和胚胎发育的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-11-10 DOI: 10.1080/10495398.2024.2422316

Mònica Ferrer-Roda, Ana Gil, Maria-Teresa Paramio, Dolors Izquierdo

{"title":"Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size.","authors":"Mònica Ferrer-Roda, Ana Gil, Maria-Teresa Paramio, Dolors Izquierdo","doi":"10.1080/10495398.2024.2422316","DOIUrl":"https://doi.org/10.1080/10495398.2024.2422316","url":null,"abstract":"Oocytes spontaneously resume meiosis following their liberation from follicles, preventing full competence acquisition. Biphasic IVM (CAPA-IVM) maintains oocytes in meiotic arrest to improve developmental competence, and it specially affects poorly developed oocytes. We assessed the effect of CAPA-IVM on oocytes from small (<3mm) and large (>3mm) follicles of prepubertal goats. Oocytes were cultured for 6h in pre-IVM with C-type natriuretic peptide (CNP) and estradiol as meiotic inhibitors, and germinal vesicle (GV) rate and chromatin configuration were assessed. Then, oocytes were cultured in conventional IVM (c-IVM) or CAPA-IVM (pre-IVM + c-IVM) and EGF receptor (EGFR) protein expression, intra-oocyte ROS and blastocyst development were assessed. GV rate was higher in CNP groups than control (69% vs 28%, and 67% vs 31%, small and large follicles, respectively), but GV chromatin configuration was similar. In large follicles, EGFR expression was higher in oocytes and cumulus cells after CAPA-IVM, and ROS levels were lower. In small follicles these differences were not observed. c-IVM and CAPA-IVM produced similar blastocyst rates in small (3.7% vs 2.6%, respectively) and large follicles (8.3% vs 2.5%). Overall, CAPA-IVM enhanced EGFR expression for EGF peptide signalling and antioxidant capacity in oocytes from large follicles but oocytes from small follicles were too immature to benefit from it.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2422316"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels. 多组织基因表达谱分析具有低和高牛奶尿素水平遗传易感性的奶牛。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-03-01 DOI: 10.1080/10495398.2024.2322542

Henry Reyer, Hanne Honerlagen, Michael Oster, Siriluck Ponsuksili, Björn Kuhla, Klaus Wimmers

{"title":"Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels.","authors":"Henry Reyer, Hanne Honerlagen, Michael Oster, Siriluck Ponsuksili, Björn Kuhla, Klaus Wimmers","doi":"10.1080/10495398.2024.2322542","DOIUrl":"10.1080/10495398.2024.2322542","url":null,"abstract":"Milk urea (MU) concentration is proposed as an indicator trait for breeding toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 German Holstein cows based on MU breeding values, with 10 cows each having low (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we characterized these cows to unravel molecular pathways governing post-absorptive body N pools focusing on renal filtration and reabsorption of nitrogenous compounds, hepatic urea formation and mammary gland N excretion. While we observed minor adjustments in cellular energy metabolism in different tissues associated with different MU levels, no transcriptional differences in liver ammonia detoxification were detected, despite significant differences in MU between the groups. Differential expression of AQP3 and SLC38A2 in the kidney provides evidence for higher urea concentration in the collecting duct of LMU cows than HMU cows. The mammary gland exhibited the most significant differences, particularly in tricarboxylic acid (TCA) cycle genes, amino acid transport, tRNA binding, and casein synthesis. These findings suggest that selecting for lower MU could lead to altered urinary urea (UU) handling and changes in milk protein synthesis. However, given the genetic variability in N metabolism components, the long-term effectiveness of MU-based selection in reducing N emissions remains uncertain.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2322542"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of AGO2 and PLEC genes polymorphisms in Hu sheep and their relationship with body size traits. 胡羊 AGO2 和 PLEC 基因多态性的鉴定及其与体型特征的关系

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-27 DOI: 10.1080/10495398.2023.2295926

Jia Liu, Wenxin Zheng, Weimin Wang, Xiaobin Yang, Yongliang Huang, Panpan Cui, Zongwu Ma, Xiwen Zeng, Rui Zhai, Xiuxiu Weng, Weiwei Wu, Xiaoxue Zhang

{"title":"Identification of AGO2 and PLEC genes polymorphisms in Hu sheep and their relationship with body size traits.","authors":"Jia Liu, Wenxin Zheng, Weimin Wang, Xiaobin Yang, Yongliang Huang, Panpan Cui, Zongwu Ma, Xiwen Zeng, Rui Zhai, Xiuxiu Weng, Weiwei Wu, Xiaoxue Zhang","doi":"10.1080/10495398.2023.2295926","DOIUrl":"10.1080/10495398.2023.2295926","url":null,"abstract":"The body size traits are major traits in livestock, which intuitively displays the development of the animal's bones and muscles. This study used PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR) to analyze the Single-nucleotide polymorphism and expression characteristics of Argonaute RISC catalytic component 2 (AGO2) and Plectin (PLEC) genes in Hu sheep. Two intron mutations were found in Hu sheep, which were AGO2 g.51700 A > C and PLEC g.23157 C > T, respectively. Through association analysis of two mutation sites and body size traits, it was found that AGO2 g.51700 A > C mainly affects the chest and cannon circumference of Hu sheep of while PLEC g.23157 C mainly affects body height and body length. The combined genotypes of AGO2 and PLEC genes with body size traits showed SNPs at the AGO2 g.51700 A > C and PLEC g.23157 C > T loci significantly improved the body size traits of Hu sheep. In addition, the AGO2 gene has the highest expression levels in the heart, rumen, and tail fat, and the PLEC gene is highly expressed in the heart. These two loci can provide new research ideas for improving the body size traits of Hu sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2295926"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of selenium-enriched yeast dietary supplementation on egg quality, gut morphology and caecal microflora of laying hens. 富硒酵母膳食补充剂对蛋鸡鸡蛋质量、肠道形态和盲肠微生物区系的影响

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10495398.2023.2258188

Ruili Li, Jiewei Liu, Minxiao Liu, Mingzhi Liang, Zengguang Wang, Yufen Sha, Huiwen Ma, Yafeng Lin, Baohua Li, Jinming You, Lei Zhang, Ming Qin

{"title":"Effects of selenium-enriched yeast dietary supplementation on egg quality, gut morphology and caecal microflora of laying hens.","authors":"Ruili Li, Jiewei Liu, Minxiao Liu, Mingzhi Liang, Zengguang Wang, Yufen Sha, Huiwen Ma, Yafeng Lin, Baohua Li, Jinming You, Lei Zhang, Ming Qin","doi":"10.1080/10495398.2023.2258188","DOIUrl":"10.1080/10495398.2023.2258188","url":null,"abstract":"Selenium (Se) is an essential micronutrient for humans and animals and is a powerful antioxidant that can promote reproductive and immune functions. The purpose of this study was to evaluate the effects of supplemental dietary selenium-enriched yeast (SeY) on egg quality, gut morphology and microflora in laying hens. In total, 100 HY-Line Brown laying hens (45-week old) were randomly allocated to two groups with 10 replicates and fed either a basal diet (without Se supplementation) or a basal diet containing 0.2 mg/kg Se in the form of SeY for 8 weeks. The Se supplementation did not have a significant effect on egg quality and intestinal morphology of laying hens. Based on the 16S rRNA sequencing, SeY dietary supplementation effectively modulated the cecal microbiota structure. An alpha diversity analysis demonstrated that birds fed 100 mg/kg SeY had a higher cecal bacterial diversity. SeY dietary addition elevated Erysipelotrichia (class), Lachnospiraceae (family), Erysipelotrichaceae (family) and Ruminococcus_torques_group (genus; p < .05). Analysis of microbial community-level phenotypes revealed that SeY supplementation decreased the microorganism abundance of facultatively anaerobic and potentially pathogenic phenotypes. Overall, SeY supplementation cannot significantly improve intestinal morphology; however, it modulated the composition of cecal microbiota toward a healthier gut.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2258188"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes. OCT4和PGP9.5基因在水牛产前和产后睾丸中的表达分析。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-11-25 DOI: 10.1080/10495398.2023.2285509

Ashritha Q Carlo, Devendra Pathak, Ratan K Choudhary, Opinder Singh, Neelam Bansal

{"title":"Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (Bubalus bubalis) testes.","authors":"Ashritha Q Carlo, Devendra Pathak, Ratan K Choudhary, Opinder Singh, Neelam Bansal","doi":"10.1080/10495398.2023.2285509","DOIUrl":"10.1080/10495398.2023.2285509","url":null,"abstract":"This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative expression of the OCT4 gene was down-regulated in the adult testes compared to its expression during prepubertal and neonatal life. The relative expression of the PGP9.5 gene was up-regulated in the neonatal testes and down-regulated in the prepubertal and adult testes. The spermatogonia were round, oval-to-ellipsoidal cells lying over the basement membrane (BM) with a round-to-oval nucleus. Based on the immunoexpression of the putative SSC markers, OCT4 and PGP9.5, we concluded that the proportion of stem cells was highest during the neonatal stage, followed by the prepubertal and prenatal stages. This finding sheds light on the dynamics of spermatogonial stem cells in buffalo testes at different developmental stages, providing valuable insights into these cells' regulation and potential applications.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2285509"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138440222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0