Animal Biotechnology最新文献

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The impact of genetic variants related to the fatty acid metabolic process pathway on milk production traits in Jersey cows. 与脂肪酸代谢过程途径有关的遗传变异对泽西奶牛产奶特性的影响。
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI: 10.1080/10495398.2024.2396421
Sena Ardicli, Nursen Senturk, Berkay Bozkurt, Huseyn Babayev, Tuğçe Selvi, Stephen Skolnick, Hivdanur Ter, Beyza Aktas, Ayse Isık, Ozgur Toprak Ay, Ozge Ardicli, Ozden Cobanoglu
{"title":"The impact of genetic variants related to the fatty acid metabolic process pathway on milk production traits in Jersey cows.","authors":"Sena Ardicli, Nursen Senturk, Berkay Bozkurt, Huseyn Babayev, Tuğçe Selvi, Stephen Skolnick, Hivdanur Ter, Beyza Aktas, Ayse Isık, Ozgur Toprak Ay, Ozge Ardicli, Ozden Cobanoglu","doi":"10.1080/10495398.2024.2396421","DOIUrl":"10.1080/10495398.2024.2396421","url":null,"abstract":"<p><p>The synthesis of fatty acids plays a critical role in shaping milk production characteristics in dairy cattle. Thus, identifying effective haplotypes within the fatty acid metabolism pathway will provide novel and robust insights into the genetics of dairy cattle. This study aimed to comprehensively examine the individual and combined impacts of fundamental genes within the fatty acid metabolic process pathway in Jersey cows. A comprehensive phenotypic dataset was compiled, considering milk production traits, to summarize a cow's productivity across three lactations. Genotyping was conducted through PCR-RFLP and Sanger sequencing, while the association between genotype and phenotype was quantified using linear mixed models. Moderate biodiversity and abundant variation suitable for haplotype analysis were observed across all examined markers. The individual effects of the <i>FABP3</i>, <i>LTF</i> and <i>ANXA9</i> genes significantly influenced both milk yield and milk fat production. Additionally, this study reveals novel two-way interactions between genes in the fatty acid metabolism pathway that directly affect milk fat properties. Notably, we identified that the GGAAGG haplotype in <i>FABP3</i>×<i>LTF</i>×<i>ANXA9</i> interaction may be a robust genetic marker concerning both milk fat yield and percentage. Consequently, the genotype combinations highlighted in this study serve as novel and efficient markers for assessing the fat content in cow's milk.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2396421"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methylome and transcriptome joint analyses identify differentially expressed genes based on purebred and crossbred Tianjin-monkey Chicken. 基于纯种和杂交天津猴鸡的甲基组和转录组联合分析鉴定差异表达基因
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-09-18 DOI: 10.1080/10495398.2024.2397812
Shuli Xia, Guohui Li, Xianghua Zhao, Chenghao Zhou, Haitao Yu, Wei Han
{"title":"Methylome and transcriptome joint analyses identify differentially expressed genes based on purebred and crossbred Tianjin-monkey Chicken.","authors":"Shuli Xia, Guohui Li, Xianghua Zhao, Chenghao Zhou, Haitao Yu, Wei Han","doi":"10.1080/10495398.2024.2397812","DOIUrl":"https://doi.org/10.1080/10495398.2024.2397812","url":null,"abstract":"<p><p>Tianjin-monkey Chicken is a locally bred naked neck poultry with limited population size. Herein, we intended to identify potential breed-related genes based on methylome and transcriptome analyses. Tianjin-monkey Chicken and Hy-line Brown Chicken were crossbred and the individuals were divided into three groups: PN (Purebred naked neck chicken(Tianjin-monkey Chicken)) group (n = 10); CN (Crossbred naked neck chicken) group (n = 10); CF (Crossbred feathered chicken) group (n = 10). These 30 individuals were subjected to whole genome bisulfite sequencing (WGBS) and transcriptome sequencing. Differential methylated regions were detected by WGBS. No significant difference existed in naked-neck-related traits between PN and CN chicken. CpG methylation level of the promoter region differed in PN, CN vs. CF chicken. By integrating methylome and transcriptome results, four genes were identified between PN and CN groups, and 24 key genes were identified between CN and CF groups, with great potential in breeding. The 24 genes were enriched on 32 GO terms and 3 KEGG pathways, such as ion transport. The promoter region CpG methylation level was distinct between feathered and naked neck groups. We identified 24 potential genes for future breeding, valuable for targeted breeding and genetic enhancement in poultry production.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2397812"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction between adipocytes and macrophages participates in chick subcutaneous adipose tissue angiogenesis under cold stress conditions. 脂肪细胞和巨噬细胞之间的相互作用参与了冷应激条件下小鸡皮下脂肪组织的血管生成。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-09-17 DOI: 10.1080/10495398.2024.2400212
Yuelang Zhang,Jingxuan Li,Shanhe Wang,Mingli Wu,Haidong Zhao
{"title":"Interaction between adipocytes and macrophages participates in chick subcutaneous adipose tissue angiogenesis under cold stress conditions.","authors":"Yuelang Zhang,Jingxuan Li,Shanhe Wang,Mingli Wu,Haidong Zhao","doi":"10.1080/10495398.2024.2400212","DOIUrl":"https://doi.org/10.1080/10495398.2024.2400212","url":null,"abstract":"Previous studies have shown that subcutaneous adipose tissue is an important energy supply organ for chicks before and after birth, except yolk. So far, the significance of large deposits of subcutaneous adipose tissue in chicks is unclear. Therefore, this study takes the information interaction between adipocytes and macrophages as the starting point to explore whether adipocytes and macrophages could participate in adipose tissue fibrosis, angiogenesis, adaptive thermogenesis and other related functions in a specific metabolic environment. Under cold stress, the expression levels of genes related to lipidolysis, lipid transport and fatty acid oxidation in adipose tissue of chicks were significantly increased, but the expression levels of genes related to mitochondrial uncoupling were not significantly changed. Through Masson staining of adipose tissue of chicks under cold stress, it was found that the level of vascularization in adipose tissue of chicks was significantly increased. We found that the interaction between adipocyte and macrophage could participate in the angiogenesis related process of adipocytes in chicks through the HIF1A-VEGFA pathway. The analysis of lipid metabolism in subcutaneous adipose tissue of chicks from the perspective of cell heterogeneity will expand the understanding of lipid metabolism in chicks and provide a theoretical basis for chick rearing.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"53 1","pages":"2400212"},"PeriodicalIF":3.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sequencing and characterization of complete mitogenome DNA of worldwide turkey (Meleagris gallopavo) populations. 全球火鸡(Meleagris gallopavo)种群完整有丝分裂基因组 DNA 的测序和特征描述。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10495398.2024.2397682
Carlotta Ferrari,Stefano P Marelli,Alessandro Bagnato,Silvia Cerolini,Maria G Strillacci
{"title":"Sequencing and characterization of complete mitogenome DNA of worldwide turkey (Meleagris gallopavo) populations.","authors":"Carlotta Ferrari,Stefano P Marelli,Alessandro Bagnato,Silvia Cerolini,Maria G Strillacci","doi":"10.1080/10495398.2024.2397682","DOIUrl":"https://doi.org/10.1080/10495398.2024.2397682","url":null,"abstract":"The history of turkey (Meleagris gallopavo) domestication can be traced back to the period between 700 and 200 BC in Mexico. This process involved multiple contributors and resulted in the development of modern local turkey breeds. This research investigates the complete mitochondrial diversity across a diverse range of local turkeys. Seventy-three turkeys were sampled from various populations, including autochthonous Italian breeds, an American breed (Narragansett), as well as wild turkeys from the USA and Mexico. The mitochondrial DNA (mtDNA) was employed as a powerful tool for biodiversity and breed phylogeny investigation. An analysis of the entire mtDNA was conducted to identify breed-specific unique traits, mitochondrial-specific characteristics, and the phylogenetic relationship among turkey populations. A total of 44 polymorphic sites were identified. Brianzolo and Narragansett birds were characterized as genetically homogeneous populations. Thirty-two different haplotypes were identified when our samples were compared with mtDNA D-loop of 96 online available turkeys from various geographical countries. H1 and H2, differing for one mutation, were the most abundant, comprising 132 of the 185 sequences. H1 included samples coming from every region, while H2 was predominantly characterized by Italian samples. USA and Mexican samples appear to be more variable in their mtDNA than the other populations.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"10 1","pages":"2397682"},"PeriodicalIF":3.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142201141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular identification of haemoparasites in animals using blood lysate PCR: a quick and inexpensive alternative to purified whole genomic DNA. 利用血液裂解液 PCR 对动物血液寄生虫进行分子鉴定:一种快速、廉价的全基因组 DNA 纯化替代方法。
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-08-09 Epub Date: 2024-08-13 DOI: 10.1080/10495398.2024.2390935
Binod Kumar, Nilima N Brahmbhatt, Bhupendrakumar Thakre, Biswa Ranjan Maharana, Vijay L Parmar, Manoj Kumar
{"title":"Molecular identification of haemoparasites in animals using blood lysate PCR: a quick and inexpensive alternative to purified whole genomic DNA.","authors":"Binod Kumar, Nilima N Brahmbhatt, Bhupendrakumar Thakre, Biswa Ranjan Maharana, Vijay L Parmar, Manoj Kumar","doi":"10.1080/10495398.2024.2390935","DOIUrl":"https://doi.org/10.1080/10495398.2024.2390935","url":null,"abstract":"<p><p>Haemoparasitic diseases constitute a significant constraint to economic livestock farming. Diagnostic techniques that are inexpensive, rapid, reliable, and precise are crucial for the management of diseases. In this context, PCR assays are very valuable yet expensive since the samples must be processed before being included in the PCR reaction. Accordingly, the goal of the current study was to lower the PCR costs without jeopardizing the assay's sensitivity and specificity. For that purpose, the alkaline solution was optimized for low cost and quick DNA extraction (blood lysate), and PCR reagents were modified for optimum reaction. In comparison to purified whole blood genomic DNA, the currently developed and optimized blood lysate method was found to be 95.5% less expensive, as well as being equally sensitive and specific for the molecular detection (PCR) of haemoparasites like <i>Babesia, Theileria</i>, <i>Trypanosoma</i> and rickettsiales in cattle, buffaloes, horses, and dogs. The assay was also demonstrated to be quick, less likely to cross-contaminate, and appropriate for use in laboratories with limited resources. Therefore, the currently developed and optimized blood lysate method could serve as a viable alternative to purified whole blood genomic DNA for molecular detection (PCR) of haemoparasites in animals particularly in resource-limited settings.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2390935"},"PeriodicalIF":1.7,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ASReml-based estimation of early genetic parameters in hybrid mutton sheep populations. 基于 ASReml 的杂交羊群早期遗传参数估计。
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-07-22 Epub Date: 2024-08-02 DOI: 10.1080/10495398.2024.2383261
Haifeng Wang, Shanning Jin, Zhenfei Xu, Jinxia Zhang, Xuejiao An, Rui Zhang, Yanyan Hao, Lina Zhu, Yinchun Wang, Junni Jin, Zhiguang Geng, Chenglan Li, Jianye Li, Yaojing Yue
{"title":"ASReml-based estimation of early genetic parameters in hybrid mutton sheep populations.","authors":"Haifeng Wang, Shanning Jin, Zhenfei Xu, Jinxia Zhang, Xuejiao An, Rui Zhang, Yanyan Hao, Lina Zhu, Yinchun Wang, Junni Jin, Zhiguang Geng, Chenglan Li, Jianye Li, Yaojing Yue","doi":"10.1080/10495398.2024.2383261","DOIUrl":"https://doi.org/10.1080/10495398.2024.2383261","url":null,"abstract":"<p><p>The aim of this study was to analyze the effects of non-genetic factors on the estimation of genetic parameters of early growth traits in hybrid mutton sheep using ASReml software, in order to provide theoretical basis for screening the optimal hybriding combinations and accelerating the breeding process of new breeds of specialized housed-feeding mutton sheep. We selected the wellgrown hybrid Southhu (Southdown × Hu sheep) and Dorhu (Dorset × Hu sheep) sheep as the research objects, constructed weight correction formulae for SH and DH sheep at 60 and 180 days; and used ASReml software to investigate the effects of non-genetic factors on the estimation of genetic parameters of early growth traits in hybrid sheep. The results showed that the birth month and birth type were found significant for all traits (<i>p</i> < 0.001); the heritability of maternal effects ranged from 0.0709 to 0.1859. It was found that both SH and DH sheep emerged the potential for rapid early growth and development, early growth traits are significantly affected by maternal genetic effects, thereby the maternal effect should be taken into consideration for the purpose of improving accuracy in parameter estimations and therefore increasing the success of breeding programs.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2383261"},"PeriodicalIF":1.7,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A meta-analysis approach for annotation and identification of lncRNAs controlling perirenal fat deposition in suckling lambs. 控制哺乳羔羊肾周脂肪沉积的lncRNA的注释和鉴定的荟萃分析方法
IF 1.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-06-28 Epub Date: 2024-07-14 DOI: 10.1080/10495398.2024.2374328
María Alonso-García, Beatriz Gutiérrez-Gil, Rocío Pelayo, Pablo A S Fonseca, Héctor Marina, Juan José Arranz, Aroa Suárez-Vega
{"title":"A meta-analysis approach for annotation and identification of lncRNAs controlling perirenal fat deposition in suckling lambs.","authors":"María Alonso-García, Beatriz Gutiérrez-Gil, Rocío Pelayo, Pablo A S Fonseca, Héctor Marina, Juan José Arranz, Aroa Suárez-Vega","doi":"10.1080/10495398.2024.2374328","DOIUrl":"10.1080/10495398.2024.2374328","url":null,"abstract":"<p><p>Long non-coding RNAs (lncRNAs) are being studied in farm animals due to their association with traits of economic interest, such as fat deposition. Based on the analysis of perirenal fat transcriptomes, this research explored the relevance of these regulatory elements to fat deposition in suckling lambs. To that end, meta-analysis techniques have been implemented to efficiently characterize and detect differentially expressed transcripts from two different RNA-seq datasets, one including samples of two sheep breeds that differ in fat deposition features, Churra and Assaf (<i>n</i> = 14), and one generated from Assaf suckling lambs with different fat deposition levels (<i>n</i> = 8). The joint analysis of the 22 perirenal fat RNA-seq samples with the <i>FEELnc</i> software allowed the detection of 3953 novel lncRNAs. After the meta-analysis, 251 differentially expressed genes were identified, 21 of which were novel lncRNAs. Additionally, a co-expression analysis revealed that, in suckling lambs, lncRNAs may play a role in controlling angiogenesis and thermogenesis, processes highlighted in relation to high and low fat deposition levels, respectively. Overall, while providing information that could be applied for the improvement of suckling lamb carcass traits, this study offers insights into the biology of perirenal fat deposition regulation in mammals.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2374328"},"PeriodicalIF":1.7,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retinoic acid signalling inhibits myogenesis by blocking MYOD translation in pig skeletal muscle cells. 维甲酸信号通过阻断猪骨骼肌细胞中 MYOD 的翻译来抑制肌生成。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-05-08 Epub Date: 2024-05-16 DOI: 10.1080/10495398.2024.2351973
Changying Wang, Ruige Liu, Wenzhe Luo, Pengxiang Zhao, Heng Wang
{"title":"Retinoic acid signalling inhibits myogenesis by blocking MYOD translation in pig skeletal muscle cells.","authors":"Changying Wang, Ruige Liu, Wenzhe Luo, Pengxiang Zhao, Heng Wang","doi":"10.1080/10495398.2024.2351973","DOIUrl":"10.1080/10495398.2024.2351973","url":null,"abstract":"<p><p>Vitamin A is an essential nutrient in animals, playing important roles in animal health. In the pig industry, proper supplementation of vitamin A in the feed can improve pork production performance, while deficiency or excessive intake can lead to growth retardation or disease. However, the specific molecular mechanisms through which vitamin A operates on pig skeletal muscle growth as well as muscle stem cell function remain unexplored. Therefore, in this study, we isolated the pig primary skeletal muscle stem cells (pMuSCs) and treated with retinoic acid (RA), the natural metabolite of vitamin A, and then examined the myogenic capacity of pMuSCs via immunostaining, real-time PCR, CCK8 and western-blot analysis. Unexpectedly, the RA caused a significant decrease in the proliferation and differentiation of pMuSCs. Mechanistically, the RA addition induced the activation of retinoic acid receptor gamma (RARγ), which inhibited the myogenesis through the blockage of protein translation of the master myogenic regulator myogenic differentiation 1 gene (MYOD). Specifically, RARγ inactivate AKT kinase (AKT) signalling and lead to dephosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (eIF4EBP1), which in turn repress the eukaryotic translation initiation factor 4E (eIF4E) complex and block mRNA translation of MYOD. Inhibition of AKT could rescue the myogenic defects of RA-treated pMuSCs. Our findings revealed that retinoid acid signalling inhibits the skeletal muscle stem cell proliferation and differentiation in pigs. Therefore, the vitamin A supplement in the feedstuff should be cautiously optimized to avoid the potential adverse consequences on muscle development associated with the excessive levels of retinoic acid.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2351973"},"PeriodicalIF":3.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Variation in the AA-NAT gene G203A is associated with Awassi and Hamdani sheep fertility. AA-NAT 基因 G203A 的变异与阿瓦西羊和哈姆达尼羊的繁殖力有关。
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-05-06 Epub Date: 2024-05-16 DOI: 10.1080/10495398.2024.2352771
Waleed A Abd Al-Jabar, Tahreer M Al-Thuwaini
{"title":"Variation in the <i>AA-NAT</i> gene G203A is associated with Awassi and Hamdani sheep fertility.","authors":"Waleed A Abd Al-Jabar, Tahreer M Al-Thuwaini","doi":"10.1080/10495398.2024.2352771","DOIUrl":"10.1080/10495398.2024.2352771","url":null,"abstract":"<p><p>Arylalkylamine-N-acetyl-transferase (<i>AA-NAT</i>) is one of several genes that influence sheep reproduction. Thus, the objective of this study was to investigate whether genetic variability within the <i>AA-NAT</i> gene influenced the reproductive performance of Awassi and Hamdani ewes. A total of 99 twin and 101 single-progeny ewes were analyzed for genomic DNA. Polymerase chain reaction (PCR) was used to produce amplicons of 300, 313, and 287 bp from exons 1, 2, and 3 of the <i>AA-NAT</i> gene. A 300-bp amplicon was genotyped, resulting in two genotypes: GG and GA. Through sequence analysis, a mutation 203 G > A was identified in the GA genotype. The statistical analysis revealed a strong correlation between the single nucleotide polymorphism (SNP) 203 G > A and reproductive performance. Ewes carrying this mutation showed significantly increased litter sizes, twinning rates, lambing rates, and fewer days to lambing compared to those carrying GG. These findings demonstrate that the presence of the 203 G > A SNP variant has a significant positive impact on litter sizes and enhances the fertility of Awassi and Hamdani sheep.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2352771"},"PeriodicalIF":3.7,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak 阿什丹牦牛睾丸发育过程中 LYZL4、LYZL6 和 PCNA 的表达和定位随年龄的变化
IF 3.7 3区 农林科学
Animal Biotechnology Pub Date : 2024-04-26 DOI: 10.1080/10495398.2024.2344213
Yongfu La, Zhongbang Li, Xiaoming Ma, Pengjia Bao, Min Chu, Xian Guo, Chunnian Liang, Ping Yan
{"title":"Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak","authors":"Yongfu La, Zhongbang Li, Xiaoming Ma, Pengjia Bao, Min Chu, Xian Guo, Chunnian Liang, Ping Yan","doi":"10.1080/10495398.2024.2344213","DOIUrl":"https://doi.org/10.1080/10495398.2024.2344213","url":null,"abstract":"Lysozyme like 4 (LYZL4), lysozyme like 6 (LYZL6) and proliferating cell nuclear antigen (PCNA) are implicated in the regulation of testicular function, but there was no research reported available ...","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140838366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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