Polymorphism in IGFALS gene and its association with scrotal circumference in Hu lambs.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-04 DOI:10.1080/10495398.2023.2295928

Zongwu Ma, Weimin Wang, Deyin Zhang, Xinji Wang, Shirong Li, Liming Zhao, Yukun Zhang, Yuan Zhao, Xiaolong Li, Changchun Lin, Jianghui Wang, Jiangbo Cheng, Dan Xu, Xiaobin Yang, Yongliang Huang, Panpan Cui, Jia Liu, Xiwen Zeng, Rui Zhai, Zhiqiang Huang, Xiuxiu Weng, Xiaoxue Zhang

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引用次数: 0

Abstract

Scrotal circumference is an important reproductive index of breeding rams, which has a high genetic correlation with ejaculation volume and semen quality. In this study, the scrotal circumference of 1353 male Hu sheep at different stages of development was measured and descriptive statistical analysis was performed. The results showed that the coefficient of variation of scrotal circumference at each stage was greater than 10%, and its heritability were moderately to high, ranging from 0.318 to 0.719. We used PCR amplification and Sanger sequencing to scan the polymorphisms of the IGFALS gene, and performed association analysis with the circumference of the scrotum at different stages. We identified a synonymous mutation g.918 G > C in exon 1 of the IGFALS gene, and this mutation was significantly associated with scrotal circumference at 100, 120, 140, 160 and 180 days (p < 0.05). Therefore, IGFALS gene polymorphism can be used as a molecular marker affecting scrotal circumference of Hu sheep, which can provide a reference for future molecular marker-assisted selection of scrotal circumference in sheep.

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IGFALS基因的多态性及其与胡羔羊阴囊周长的关系

阴囊周长是种公羊的一项重要繁殖指标，与射精量和精液质量有很高的遗传相关性。本研究测量了 1353 只雄性胡羊在不同发育阶段的阴囊周长，并进行了描述性统计分析。结果表明，各阶段阴囊周长的变异系数均大于 10%，遗传率为 0.318 至 0.719，属于中高遗传率。我们利用 PCR 扩增和 Sanger 测序技术扫描了 IGFALS 基因的多态性，并进行了与不同阶段阴囊周长的关联分析。我们在 IGFALS 基因外显子 1 中发现了一个 g.918 G > C 的同义突变，该突变与绵羊 100、120、140、160 和 180 日龄的阴囊周长显著相关（p IGFALS 基因多态性可作为影响胡羊阴囊周长的分子标记，为将来分子标记辅助选择绵羊阴囊周长提供参考）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Biotechnology 工程技术-奶制品与动物科学

CiteScore

2.90

自引率

5.40%

发文量

230

审稿时长

>12 weeks

期刊介绍： Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes