Animal Biotechnology最新文献

{"title":"Dietary ginger (<i>Zingiber officinale</i>) enhances performance traits, biochemical and haematological indices of Turkey targeting mRNA gene expression.","authors":"Eman A Manaa, Mervat A Abdel-Latif, Samya E Ibraheim, Abdelaziz M Sakr, Hanaa M Ghanem, Rania M Waheed, Ghadeer M Albadrani, Mohamed M Abdel-Daim, Amr El Zawily, Basant M Shafik","doi":"10.1080/10495398.2024.2425656","DOIUrl":"https://doi.org/10.1080/10495398.2024.2425656","url":null,"abstract":"<p><p>Ginger rich in polyphenols, possesses various biomedical properties. Researchers investigated the effects of dietary ginger supplementation on turkey performance traits, biochemical parameters, haematological parameters and mRNA gene expression. Ginger root powder was administered at different doses (0, 10, 20 and 40 g/kg) to the turkeys. Notably, the 20 g/kg group exhibited improved performance traits and a higher broiler production efficiency factor (BPEF). Importantly, ginger was found to be safe for turkeys based on serum indices. Furthermore, the expression of several growth-related genes, including growth hormone receptor (GHR), insulin-like growth factor 1 (IGF-1), adenine nucleotide translocase (ANT), cyclooxygenase 3 (COX-3) and uncoupling protein 3 (UCP-3), was upregulated in the 20 g/kg enhancing their growth performance and economic efficiency in addition to keeping their health status safe. Therefore, Ginger root powder can be supplemented for turkey at a concentration of 2% as the addition of ginger powder is a long-term process.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2425656"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus.","authors":"Chongfa Yang, Yahua Yang, Bingzhu Zhao, Enyu Gao, Hao Chen, Yang Li, Junyuan Ma, Jine Wang, Songming Hu, Xiaochen Song, Ying Chen, Gengsacairang Yang, Shengdong Huo, Wenxue Luo","doi":"10.1080/10495398.2024.2427757","DOIUrl":"10.1080/10495398.2024.2427757","url":null,"abstract":"<p><p>Since most yaks have a long postpartum anestrus period, postpartum anestrus is the main factor affecting the reproductive efficiency of yaks. In this study, the third-generation sequencing technology was used to successfully screen differentially expressed genes (DEGs) and differentially expressed transcripts (DETs) in the ovarian tissues of yaks during estrus and anestrus. The functional references of DEGs and DETs were Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Clusters of Orthologous Genes database. A total of 1149 DEGs and 2294 DETs were successfully identified. These DEGs and DETs were mainly related to biological processes such as \"reproduction\", \"reproductive process\", \"metabolic process\" and \"rhythmic process\". Kisspeptin-G protein-coupled receptor was found to be involved in regulating the reproductive cycle of yaks. DEGs and DETs were also related to gonadotropin-releasing hormone (GnRH) signaling pathways such as oocyte meiosis, estrogen signaling pathway, and progesterone-mediated induced oocyte maturation. The results showed that <i>SIRT1</i>, <i>CSNK1A1</i>, <i>SLIT3</i>, <i>INHBA</i>, <i>INSL3</i>, <i>ZP2</i>, <i>Clock</i>, <i>BMP15</i>, <i>Bmal1</i>, <i>KISS1</i>, and <i>LCHGR</i> regulate the postpartum quiescent state and the reproductive cycle of yaks. This study will help to further clarify the reproductive mechanism of yaks at the molecular level and provide certain assistance for the development of animal husbandry.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2427757"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring aptamers for targeted enrichment of X sperm in bovine: unraveling selective potential.","authors":"Sumit Kumar Singh, Rohit Kumar, Manya Mathur, Himanshu Kamboj, Jai Kumar Kaushik, Ashok Kumar Mohanty, Sudarshan Kumar","doi":"10.1080/10495398.2024.2323592","DOIUrl":"https://doi.org/10.1080/10495398.2024.2323592","url":null,"abstract":"<p><p>Nucleic acid aptamers have been used in the past for the development of diagnostic methods against a number of targets such as bacteria, pesticides, cancer cells etc. In the present study, six rounds of Cell-SELEX were performed on a ssDNA aptamer library against X-enriched sperm cells from Sahiwal breed cattle. Sequencing was used to examine the aptamer sequences that shown affinity for sperm carrying the X chromosome in order to find any possible X-sperm-specific sequences. Out of 35 identified sequences, 14 were selected based on bioinformatics analysis like G-Score and Mfold structures. Further validation of their specificity was done via fluorescence microscopy. The interaction of biotinylated-aptamer with sperm was also determined by visualizing the binding of streptavidin coated magnetic beads on the head region of the sperm under bright field microscopy. Finally, a real-time experiment was designed for the validation of X-sperm enrichment by synthesized aptamer sequences. Among the studied sequences, aptamer 29a exhibited a higher affinity for X sperm compared to Y sperm in a mixed population of sperm cells. By using aptamer sequence 29a, we obtained an enrichment of 70% for X chromosome bearing sperm cells.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2323592"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular cloning of <i>TPM3</i> gene in qinchuan cattle and its effect on myoblast proliferation and differentiation.","authors":"Juntao Guo, Jianfang Wang, Ke Zhang, Zhimei Yang, Bingzhi Li, Yueting Pan, Hengwei Yu, Shengchen Yu, Sayed Haidar Abbas Raza, Belete Kuraz Abebea, Linsen Zan","doi":"10.1080/10495398.2024.2345238","DOIUrl":"10.1080/10495398.2024.2345238","url":null,"abstract":"<p><p>Tropomyosin 3 (<i>TPM3</i>) plays a significant role as a regulatory protein in muscle contraction, affecting the growth and development of skeletal muscles. Despite its importance, limited research has been conducted to investigate the influence of <i>TPM3</i> on bovine skeletal muscle development. Therefore, this study revealed the role of <i>TPM3</i> in bovine myoblast growth and development. This research involved conducting a thorough examination of the Qinchuan cattle <i>TPM3</i> gene using bioinformatics tools to examine its sequence and structural characteristics. Furthermore, <i>TPM3</i> expression was evaluated in various bovine tissues and cells using quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that the coding region of <i>TPM3</i> spans 855 bp, with the 161st base being the T base, encoding a protein with 284 amino acids and 19 phosphorylation sites. This protein demonstrated high conservation across species while displaying a predominant α-helix secondary structure despite being an unstable acidic protein. Notably, a noticeable increase in <i>TPM3</i> expression was observed in the longissimus dorsi muscle and myocardium of calves and adult cattle. Expression patterns varied during different stages of myoblast differentiation. Functional studies that involved interference with <i>TPM3</i> in Qinchuan cattle myoblasts revealed a very significantly decrease in S-phase cell numbers and EdU-positive staining (<i>P</i> < 0.01), and disrupted myotube morphology. Moreover, interference with <i>TPM3</i> resulted in significantly (<i>P</i> < 0.05) or highly significantly (<i>P</i> < 0.01) decreased mRNA and protein levels of key proliferation and differentiation markers, indicating its role in the modulation of myoblast behavior. These findings suggest that <i>TPM3</i> plays an essential role in bovine skeletal muscle growth by influencing myoblast proliferation and differentiation. This study provides a foundation for further exploration into the mechanisms underlying <i>TPM3</i>-mediated regulation of bovine muscle development and provides valuable insights that could guide future research directions as well as potential applications for livestock breeding and addressing muscle-related disorders.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2345238"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of miR-23b-3p on regulating GH by targeting POU1F1 in Yanbian yellow cattle.","authors":"Lu Xu, Taihua Jin, Angang Lou, Jiuyang Guan, Xinglin Zhang, Hui Wang, Lizeng Guan","doi":"10.1080/10495398.2024.2346808","DOIUrl":"10.1080/10495398.2024.2346808","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of miR-23b-3p on growth hormone (GH) in pituitary cells of Yanbian yellow cattle. The mRNA and protein levels of GH and miR-23b-3p target genes were measured by real time fluorescence quantitative PCR (qPCR) and Western blot, respectively. The target relationship of miR-23b-3p was validated by double luciferase reporter gene system. The results showed that GH mRNA and protein levels in pituitary cells of Yanbian yellow cattle were significantly lower in the miR-23b-3p-mi group than in the NC group (<i>P</i><0.01), while GH mRNA and protein levels were higher in the miR-23b-3p-in group than in the iNC group (<i>P</i><0.05). The result of bioinformatics analysis and double luciferase reporter gene system validation proved that miR-23b-3p targeted 3'UTR of pituitary specific transcription factor 1 (POU1F1). POU1F1 mRNA and protein levels were lower miR-23b-3p-mi group than in the NC group (<i>P</i><0.01), while POU1F1 mRNA and protein levels were higher in the miR-23b-3p-in group than in the iNC group (<i>P</i><0.01). These results demonstrated that miR-23b-3p could regulate GH expression in pituitary cells by regulating <i>POU1F1</i> gene.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2346808"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a garlic drench on Galectin gene expression in ovine whole blood.","authors":"SreeNavya Inupala, Md Rasel Uzzaman, Priyanka Pande, Sowmya Jagana, Mulumebet Worku","doi":"10.1080/10495398.2024.2344208","DOIUrl":"10.1080/10495398.2024.2344208","url":null,"abstract":"<p><p>Garlic, known for its immune-modulating and antibiotic properties, contains lectins that possess antimicrobial and immunomodulatory effects. Galectins (Gals), which bind β-galactosides, play a role in modulating immunity and pathological processes. It is hypothesized that garlic's lectin components interfere with animal lectins. St. Croix sheep, known for their resistance to parasites and adaptability, are influenced by dietary supplements for innate immunity. This study evaluated the impact of garlic drench on Galectin gene expression in St. Croix sheep. Adult non-lactating ewes received either garlic juice concentrate or sterile distilled water for four weeks. Blood samples were collected, and plasma and whole blood cells were separated. Galectin secretion was assessed using a Sheep-specific ELISA, while Galectin gene transcription was analyzed through real-time PCR. Garlic administration upregulated LGALS-3 gene expression and significantly increased total plasma protein concentration. Garlic supplementation also affected Galectin secretion, with Gal-1, Gal-3, and Gal-9 showing differential effects.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2344208"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphism in <i>IGFALS</i> gene and its association with scrotal circumference in Hu lambs.","authors":"Zongwu Ma, Weimin Wang, Deyin Zhang, Xinji Wang, Shirong Li, Liming Zhao, Yukun Zhang, Yuan Zhao, Xiaolong Li, Changchun Lin, Jianghui Wang, Jiangbo Cheng, Dan Xu, Xiaobin Yang, Yongliang Huang, Panpan Cui, Jia Liu, Xiwen Zeng, Rui Zhai, Zhiqiang Huang, Xiuxiu Weng, Xiaoxue Zhang","doi":"10.1080/10495398.2023.2295928","DOIUrl":"10.1080/10495398.2023.2295928","url":null,"abstract":"<p><p>Scrotal circumference is an important reproductive index of breeding rams, which has a high genetic correlation with ejaculation volume and semen quality. In this study, the scrotal circumference of 1353 male Hu sheep at different stages of development was measured and descriptive statistical analysis was performed. The results showed that the coefficient of variation of scrotal circumference at each stage was greater than 10%, and its heritability were moderately to high, ranging from 0.318 to 0.719. We used PCR amplification and Sanger sequencing to scan the polymorphisms of the <i>IGFALS</i> gene, and performed association analysis with the circumference of the scrotum at different stages. We identified a synonymous mutation g.918 G > C in exon 1 of the <i>IGFALS</i> gene, and this mutation was significantly associated with scrotal circumference at 100, 120, 140, 160 and 180 days (<i>p</i> < 0.05). Therefore, <i>IGFALS</i> gene polymorphism can be used as a molecular marker affecting scrotal circumference of Hu sheep, which can provide a reference for future molecular marker-assisted selection of scrotal circumference in sheep.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2295928"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139085566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive study on the longissius dorsi muscle of Ashdan yaks under different feeding regimes based on transcriptomic and metabolomic analyses.","authors":"Tong Wang, Xiaoming Ma, Qingbo Zheng, Chaofan Ma, Zhilong Zhang, Heping Pan, Xian Guo, Xiaoyun Wu, Min Chu, Chunnian Liang, Ping Yan","doi":"10.1080/10495398.2023.2294785","DOIUrl":"10.1080/10495398.2023.2294785","url":null,"abstract":"<p><p>Yak is an important dominant livestock species at high altitude, and the growth performance of yak has obvious differences under different feeding methods. This experiment was conducted to compare the effects of different feeding practices on growth performance and meat quality of yaks through combined transcriptomic and metabolomic analyses. In terms of yak growth performance, compared with traditional grazing, in-house feeding can significantly improve the average daily weight gain, carcass weight and net meat weight of yaks; in terms of yak meat quality, in-house feeding can effectively improve the quality of yak meat. A combined transcriptomic and metabolomic analysis revealed 31 co-enriched pathways, among which arginine metabolism, proline metabolism and glycerophospholipid metabolism may be involved in the development of the longissimus dorsi muscle of yak and the regulation of meat quality-related traits. The experimental results increased our understanding of yak meat quality and provided data materials for subsequent deep excavation of the mechanism of yak meat quality.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2294785"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxic stress caused apoptosis of MDBK cells by p53/BCL6-mitochondrial apoptosis pathway.","authors":"Bin Li, Yangjin Baima, Ji De, Dongxu Wen, Yang Liu, Zhuzha Basang, Nan Jiang","doi":"10.1080/10495398.2023.2299241","DOIUrl":"10.1080/10495398.2023.2299241","url":null,"abstract":"<p><p>Hypoxia is an important characteristic of Tibetan plateau environment. It can lead to apoptosis, but the mechanism of apoptosis caused by hypoxic stress needs further clarification. Here, cattle kidney cell MDBK were used as cell model. The effect of hypoxic stress on apoptosis and its molecular mechanism were explored. MDBK cells were treated with hypoxic stress, apoptosis and mitochondrial apoptotic pathway were significantly increased, and the expression of B-cell lymphoma 6 (BCL6) was significantly decreased. Overexpressing or inhibiting BCL6 demonstrated that BCL6 inhibited the apoptosis. And the increase of apoptosis controlled by hypoxic stress was blocked by BCL6 overexpressing. MDBK cells were treated with hypoxic stress, the expression and the nuclear localization of p53 were significantly increased. Overexpressing or inhibiting p53 demonstrated that hypoxic stress suppressed the expression of BCL6 through p53. Together, these results indicated that hypoxic stress induced the apoptosis of MDBK cells, and BCL6 was an important negative factor for this regulation process. In MDBK cells, hypoxic stress suppressed the expression of BCL6 through p53/BCL6-mitochondrial apoptotic pathway. This study enhanced current understanding of the molecular mechanisms underlying the regulation of apoptosis by hypoxic stress in MDBK cells.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2299241"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139097151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genomic regions affecting nitrogen excretion intensity in Brown Swiss dairy cows.","authors":"Žan Pečnik, Daša Jevšinek Skok","doi":"10.1080/10495398.2024.2434097","DOIUrl":"https://doi.org/10.1080/10495398.2024.2434097","url":null,"abstract":"<p><p>Dairy cows with a lower nitrogen excretion intensity (N_exi) excrete less nitrogen, ammonia (NH₃) and nitrous oxide (N₂O), a highly potent greenhouse gas (GHG), per kg of milk produced and therefore represent a lower environmental impact while maintaining food security. To date, the genomics background of N_exi is unknown. Here we performed a genetic association study, overlap analysis and functional enrichment analysis for N_exi in 875 genotyped dairy cows with 2,147 lactations from 200 herds. We identified 1456 single nucleotide polymorphisms (SNPs) that significantly affect N_exi. We found 140 SNPs overlapping with 148 protein-coding genes. The <i>MAN1A1</i> gene is a strong candidate gene for N_exi. Genotype CC of rs42786248, the most significantly associated SNP located in the <i>MAN1A1</i> gene, had higher N_exi than genotypes AA (<i>p</i> < 0.001) and AC (<i>p</i> < 0.001). We identified 33 genes involved in biological processes related to nitrogen metabolism. Our results form the basis for further research on the genomics background of N_exi. The identified SNPs serve as potential targets for selective breeding programs, aimed at reducing N_exi and associated NH₃ and N₂O emissions in cattle production, thus contributing to more environmentally sustainable milk production.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2434097"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}