Animal Biotechnology最新文献

{"title":"Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry and <i>in-silico</i> annotation.","authors":"Vanya Bhushan, Syed Azmal Ali, Abhishek Parashar, Sudarshan Kumar, Ashok Kumar Mohanty","doi":"10.1080/10495398.2024.2428402","DOIUrl":"10.1080/10495398.2024.2428402","url":null,"abstract":"<p><p>Proteomic analysis of sperm cells offers significant insights into proteins' structural, functional, and localization aspects within biological systems. Sahiwal, a native Indian cattle breed, is well known for its disease resistance, calving ease, and resilience to drought. This study addressed the gap in Sahiwal's comprehensive sperm proteome profiling data. The research involved the global in-silico quantitative high-resolution mass spectrometry-based protein profiling of Indian Zebu sperm, identifying 4651 sperm proteins. Beyond mere identification, the study characterized these proteins at a sub-organellar level to facilitate a better understanding of their functional attributes. Gene Ontology analysis of sperm proteins facilitated the segregation of proteins based on their function, localization, and mode of action. The study revealed that despite the limited number of organelles, sperm cells encapsulate a wide array of crucial proteins, compensating for the deficiency of organelles through the presence of multifunctional proteins. Most identified sperm proteins actively participate in spermatogenesis, motility, acrosome reaction, capacitation, and seminal plasma binding, directly or indirectly. Notably, the results not only present the highest number of identified bovine sperm proteins but also hold the potential to pave the way for empirical research on sperm functionality, egg-sperm interaction, sperm-sex sorting biomarkers, sperm quality, and bull fertility.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2428402"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens.","authors":"Ezgi Dinçtürk","doi":"10.1080/10495398.2023.2299733","DOIUrl":"10.1080/10495398.2023.2299733","url":null,"abstract":"<p><p>The intensive labour and time required for conventional methods to identify bacterial fish pathogens have revealed the need to develop alternative methods. Raman spectroscopy has been used in the rapid optical identification of bacterial pathogens in recent years as an alternative method in microbiology. Strains of bacterial fish pathogens <i>(Vibrio anguillarum, Lactococcus garvieae</i> and <i>Yersinia ruckeri)</i> that often cause infectious diseases in fish were here identified and analyzed in terms of their biochemical structures in different media and at different incubation times, and the data were specified by using Raman spectroscopy. The results demonstrated that Raman spectroscopy presents species-specific Raman spectra of each disease-causing bacteria and that it would be more appropriate to choose general microbiological media over selective media for routine studies. Additionally, it was found that species-specific band regions did not differ in 24- and 48-hour cultures, but there could be a difference in peak intensity which may lead to difficult characterization of spectrum. The current study, conducted for the first time with bacterial fish pathogens under different incubation conditions, is believed to provide a basis for the routine use of Raman spectroscopy for quick pathogen identification and the precise determination of the methodology for further research.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2299733"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139080440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide selection reveals candidate genes associated with multiple teats in Hu sheep.","authors":"Wen Zhou, Cheng-Long Zhang, Zhipeng Han, Xiaopeng Li, Xinyu Bai, Jieru Wang, Ruizhi Yang, Shudong Liu","doi":"10.1080/10495398.2024.2380766","DOIUrl":"10.1080/10495398.2024.2380766","url":null,"abstract":"<p><p>Increasing the number of teats in sheep helps to improve the survival rate of sheep lambs after birth. In order to analyze the candidate genes related to the formation of multiple teats in Hu sheep, the present study was conducted to investigate the genetic pattern of multiple teats in Hu sheep. In this study, based on genome-wide data from 157 Hu sheep, Fst, xp-EHH, Pi and iHS signaling were performed, and the top 5% signal regions of each analyzed result were annotated based on the Oar_v4.0 for sheep. The results show that a total of 142 SNP loci were selected. We found that <i>PTPRG, TMEM117</i> and <i>LRP1B</i> genes were closely associated with polypodium formation in Hu sheep, in addition, among the candidate genes related to polypodium we found genes such as <i>TMEM117, SLC25A21</i> and <i>NCKAP5</i> related to milk traits. The present study screened out candidate genes for the formation of multiple teats at the genomic level in Hu sheep.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2380766"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome profile analysis of granulosa cells from porcine ovarian follicles during early atresia.","authors":"Jinbi Zhang, Xinxin Qin, Caixia Wang, Yuge Zhang, Yizhe Dou, Shiyong Xu, Jingge Liu, Zengxiang Pan","doi":"10.1080/10495398.2023.2282090","DOIUrl":"10.1080/10495398.2023.2282090","url":null,"abstract":"<p><p>At various stages of ovarian follicular development, more than 99% of follicles will be eliminated through a degenerative process called atresia. The regulatory mechanisms of atresia have been elucidated to some extent, involving hormones, growth factors, cytokines, and other factors. However, the stimuli initiating atresia in follicular granulosa cells remain unknown. In this study, we isolated the granulosa cells from porcine ovarian follicles (3-5 mm diameter) divided into healthy follicles (HFs) and early atretic follicles (EAFs). We applied high-throughput RNA sequencing to identify and compare differentially expressed genes (DEGs) between HFs and EAFs. A total of 31,694 genes were detected, of which 21,806 were co-expressed in six samples, and 243 genes (<i>p</i> < 0.05; FDR < 0.05) were differentially expressed (DEGs), including 123 downregulated and 120 upregulated in EAFs. GO analysis highlighted hormone metabolism, plasma membrane localization, and transporter activity. The pathway analysis indicated that 51 DEGs, involved in steroidogenesis, cell adhesion molecules, and TGF-beta signaling pathways, were highly related to atresia. Additionally, the interaction network of DEGs (<i>p</i> < 0.01; FDR < 0.05) using STRING highlighted LHR, ACACB, and CXCR4 as central nodes. In summary, this transcriptome analysis enriched our knowledge of the shifted mechanisms in granulosa cells during early atresia and provided novel perspectives into the atresia initiation.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2282090"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138440223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Orychophragmus violaceus</i> and/or chicory forage affects performance, egg quality, sensory evaluation and antioxidative properties in native laying hens.","authors":"W Y Zhao, Q Q Zhang, Y F Zhao, C Chang, X Wang, A L Geng","doi":"10.1080/10495398.2023.2286610","DOIUrl":"10.1080/10495398.2023.2286610","url":null,"abstract":"<p><p><i>Orychophragmus violaceus</i> (OV) and chicory (<i>Cichorium intybus L.,</i> CC) can be used as fresh or dry forage for animals. To determine whether OV and/or CC have beneficial effects on performance and egg quality, a total of 1212 28-wk-old Beijing You Chicken (BYC) laying hens with similar performance were randomly allocated to 4 groups with 3 replicate pens per group, and 101 birds per pen. The birds were fed a basal diet (control), the basal diet + OV (3.507 kg/d/pen), the basal diet + CC (2.525 kg/d/pen), and the basal diet + OV + CC (OVC, 1.7535 kg/d/pen OV + 1.2625 kg/d/pen CC) for 3 wks after one wk of adaptation. The results showed that egg-laying rate was not affected by OV, CC and OVC (<i>p</i> > 0.05), but weekly average egg mass was significantly increased by OV and CC (<i>p</i> < 0.05). The feed egg ratio in the CC group (2.82) was significantly lower than that in the other three groups (<i>p</i> < 0.05). The eggshell thickness (EST), albumen height (AH) and Haugh unit (HU) were decreased by OV and CC (<i>p</i> < 0.05); while yolk color (YC) was increased in the CC and OVC groups (<i>p</i> < 0.05). Egg grade was decreased by OV (<i>p</i> < 0.05). Sensory evaluation showed that there was a trend for increased YC in OV, CC and OVC (<i>p</i> = 0.089). Serum total protein was significantly lower in OV group than those in the control and CC group (<i>p</i> < 0.05); serum albumin content was significantly decreased in OV, CC and OVC groups (<i>p</i> = 0.006). Serum glutathione peroxidase activity in CC and OVC groups was significantly higher than that in the control group (<i>p</i> < 0.05). In conclusion, the present study suggests that CC had a better effect on the performance of the native laying hens than OV. The OV and CC affected egg quality, while YC was increased in CC and OVC groups. The OVC improved YC and serum antioxidative properties of native laying hens without affecting the performance.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2286610"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138440221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpometric and molecular characterization of Surguli goat through CO1 gene in district Kohat.","authors":"Muhammad Munir Khan, Syed Muhammad Suhail, Hafiz Abdul Majid, Ijaz Ahmad, Umer Sadique, Rajwali Khan, Iftikhar Ahmad, Asim Ijaz, Khalid Khan, Farhad Ali, Muhammad Saeed Khan, Ahmed A El-Mansi","doi":"10.1080/10495398.2023.2290528","DOIUrl":"10.1080/10495398.2023.2290528","url":null,"abstract":"<p><p>The present study was designed with the aim to study morphometric characterization as well as phylogeny and diversity of the local Surguli goat at their breeding tract district Kohat through mitochondrial DNA region, i.e., Cytochrome C Oxidase Subunit One (CO1) gene. Morphometric data and blood samples were collected from thirty (30) pure goats. Morphometric analysis showed that sex had significant effect (<i>p</i> < 0.05) on body weight, body length, hearth girth and horn length while no significant effect (<i>p</i> > 0.05) was observed for other characteristics. The results also indicated that age had significant effect (<i>p</i> < 0.05) on height at rump, ear length, horn length and tail length while no significant effect (<i>p</i> > 0.05) was observed for other characteristics. The phylogenetic analysis through CO1 nucleotide sequences within nucleotide range 1-767 showed nine polymorphic sites segregating into eight haplotypes. The mean intraspecific diversity and mean interspecific diversity were calculated as 0.23 and 2.36%, respectively. Phylogenetic tree revealed that Capra Ibex and native Surguli goat have common ancestors. The morphometric and molecular results obtained from the present study can be exploited as a selection tool for breeding and overall improvement.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2290528"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association analysis of the sorting nexin 29 (SNX29) gene copy number variations with growth traits in Diannan small-ear (DSE) pigs.","authors":"Long Yang, Xiaoding Lin, Yuhan Chen, Peiya Peng, Qun Lan, Heng Zhao, Hongjiang Wei, Yulong Yin, Mei Liu","doi":"10.1080/10495398.2024.2309956","DOIUrl":"10.1080/10495398.2024.2309956","url":null,"abstract":"<p><p><i>SNX29</i> is a potential functional gene associated with meat production traits. Previous studies have shown that <i>SNX29</i> copy number variation (CNV) could be implicated with phenotype in goats. However, in Diannan small-ear (DSE) pigs, the genetic impact of <i>SNX29</i> CNV on growth traits remains unclear. Therefore, this study investigated the associations between <i>SNX29</i> CNVs (CNV10810 and CNV10811) and growth traits in 415 DSE pigs. The results revealed that the CNV10810 mutation was significantly associated with backfat thickness in DSE pigs at 12 and 15 months old (<i>P</i> < 0.05), while the CNV10811 mutation had significant effects on various growth traits at 6 and 12 months old, particularly for body weight, body height, back height and backfat thickness (<i>P</i> < 0.05 or <i>P</i> < 0.001). In conclusion, our results confirm that <i>SNX29</i> CNV plays a role in regulating growth and development in pigs, thus suggesting its potential application for pig breeding programmes.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2309956"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139690976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of chicken <i>Relaxin3</i> gene: mRNA expression and response to reproductive hormone treatment in ovarian granulosa cells, and single nucleotide polymorphisms associated with egg laying traits in hens.","authors":"Chunfeng Zhang, Yi Sun, Li Kang, Yunliang Jiang","doi":"10.1080/10495398.2024.2370810","DOIUrl":"10.1080/10495398.2024.2370810","url":null,"abstract":"<p><p>As a protein structurally similar to insulin, relaxin3 (RLN3) plays a role in promoting arousal, suppressing depressive or anxious behaviors. Two studies revealed the increase of RLN3 expression during chicken follicle selection. In this study, by real-time quantitative PCR and luciferase assay, mRNA expression and single nucleotide polymorphisms (SNPs) of chicken <i>RLN3</i> were investigated. The mRNA expression of chicken <i>RLN3</i> was higher in the granulosa cell of hierarchal follicles (Post-GCs) than that of pre-hierarchal follicles (Pre-GCs). In Pre-GCs, the mRNA expression of chicken <i>RLN3</i> was stimulated by FSH and progesterone; in Post-GCs, it was stimulated by higher concentration of estrogen and FSH, however, was inhibited by progesterone. Four SNPs including g.-655G > C, g-592G > A, g.-372T > A and g.-282G > C were identified in the critical promoter region from -1291 bp to -207 bp of chicken <i>RLN3</i>, among which g.-655G > C, and g-592G > A were associated with age at first laying and clutch size, respectively, in Zaozhuang Sunzhi chickens. At g.-655G > C and g-592G > A, allele <i>C</i> and allele <i>A</i> had higher transcriptional activity, respectively. These data suggest that RLN3 plays an important role in chicken follicle development and SNPs in its promoter region are potential DNA markers for improving egg production traits.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2370810"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of miRNA29a gene inhibits proliferation and promotes apoptosis of jejunal epithelial cells in yak.","authors":"Jianlei Jia, Erwei Zuo, Ning Li, Siyuan Kong, Pengjia Bao, Qian Chen, Ping Yan","doi":"10.1080/10495398.2024.2391520","DOIUrl":"10.1080/10495398.2024.2391520","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) were identified to be involved in various biological functions by regulating the degradation or suppressing the translation of their downstream target genes. Recent studies have identified miR-29a play a key role in functions of mammal cell differentiation, proliferation, apoptosis, and signal transduction. However, the underlying functions for miR-29a in jejunal epithelial cells biological function still to be investigated. In order to explore the yak jejunal epithelial cells proliferation and barrier dysfunction with over expression of miR-29a gene, three 0-day-old Pamir male yaks were randomly selected and slaughtered in present study, and the jejunal epithelial cells were isolated and cultured to determine yak jejunal epithelial cells proliferation and protein composition on differential expression of miR-29a gene in Pamir plateau. Here, we demonstrated that the overexpression of miR-29a gene could inhibit the proliferation of Pamir yaks jejunum epithelial cells, and contribute to the apoptosis of Pamir yaks jejunal epithelial cells with some extent. A total of 133 differentially expressed proteins were identified in different expression of miR-29a groups by label-free Mass Spectrometry (MS), which could be concluded to two predominant themes: cell proliferation and inflammatory response. Interestingly, GPR41, as a bridge protein, was contacted two predominant themes to involved in Pamir Yaks jejunal mechanical barrier PPI network, and the target proteins displayed strong mutual interactions in the complex PPI network. Overall, our study suggested that the over-expression miR-29a inhibited the jejunal epithelial cells proliferation and the expressions of specific proteins, which damaged jejunal barrier function to slow down the intestine structure and function advanced mature development during young livestock period for influence the enhanced performance of production efficiency.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2391520"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive cognition of yak (<i>Bos grunniens</i>) <i>AIFM2</i> gene and its anti-ferroptosis role in bisphenol A-induced fetal fibroblast model.","authors":"Yueyue Li, Hongmei Xu, Zifeng Ma, Qiao Li, Yan Xiong, Xianrong Xiong, Jian Li, Daoliang Lan, Wei Fu","doi":"10.1080/10495398.2024.2377209","DOIUrl":"10.1080/10495398.2024.2377209","url":null,"abstract":"<p><p>Apoptosis-inducing factor mitochondrion-associated 2 (<i>AIFM2</i>) has been identified as a gene with anti-ferroptosis properties. To explore whether <i>AIFM2</i> exerts anti-ferroptosis role in yaks (<i>Bos grunniens</i>), we cloned yak <i>AIFM2</i> gene and analyzed its biological characteristics. The coding region of <i>AIFM2</i> had 1122 bp and encoded 373 amino acids, which was conserved in mammals. Next, RT-qPCR results showed an extensive expression of <i>AIMF2</i> in yak tissues. Furthermore, we isolated yak skin fibroblasts (YSFs) and established a bisphenol A (BPA)-induced ferroptosis model to further investigate the role of <i>AIFM2</i>. BPA elevated oxidative stress (reactive oxygen species, ROS) and lipid peroxidation (malondialdehyde, MDA and BODIPY), and reduced cell viability and antioxidant capacity (glutathione, GSH), with the severity depending on the dosage. Of note, a supplement of Ferrostatin-1 (Fer), an inhibitor of ferroptosis, restored the previously mentioned indicators. Subsequently, we constructed an <i>AIFM2</i> overexpression vector and designed <i>AIFM2</i> specific interfering siRNAs, which were transfected into YSFs. The results showed that overexpressing <i>AIFM2</i> alleviated ferroptosis, characterizing by significant changes of cell viability, ROS, BODIPY, MDA and GSH. Meanwhile, interfering <i>AIFM2</i> aggravated ferroptosis, demonstrating the critical anti-ferroptosis role of the yak <i>AIFM2</i> gene. This study shed light on further exploring the molecular mechanism of <i>AIFM2</i> in plateau adaptability.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2377209"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}