Animal Biotechnology最新文献_第8页

Whole genome resequencing-based analysis of plateau adaptation in Meiren yak (Bos grunniens). 基于全基因组重测序的美仁牦牛（Bos grunniens）高原适应性分析。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI: 10.1080/10495398.2023.2298406

Tong Wang, XiaoMing Ma, ChaoFan Ma, XiaoYun Wu, Ta ZhaXi, LiXin Yin, WeiGuo Li, YuFei Li, ChunNian Liang, Ping Yan

{"title":"Whole genome resequencing-based analysis of plateau adaptation in Meiren yak (Bos grunniens).","authors":"Tong Wang, XiaoMing Ma, ChaoFan Ma, XiaoYun Wu, Ta ZhaXi, LiXin Yin, WeiGuo Li, YuFei Li, ChunNian Liang, Ping Yan","doi":"10.1080/10495398.2023.2298406","DOIUrl":"10.1080/10495398.2023.2298406","url":null,"abstract":"The Meiren yak is an important genetic resource in Gansu Province, China. In this study, we aimed to explore the evolutionary history and population structure of the genetic resource of Meiren yak and to mine the characteristic genes of Meiren yak. We analysed a total of 93 yaks of eight yak breeds based on whole genome resequencing combined with population genomics and used θπ ratio and Fst method to screen the selected sites in the genome region. The results proved that Meiren yak can be used as a potential genetic resource in Gansu Province. The genes in Meiren yak with positive selection in selection signal analysis were subjected to the Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses, which indicated that the genes were related to the adaptability to high altitude and hypoxic environment. By analysing the genetic variation of Meiren yak at the genome-wide level, this study provided a theoretical basis for genetic improvement of Meiren yak and for the development of high-quality yak resources.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2298406"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory impacts of PPARGC1A gene expression on milk production and cellular metabolism in buffalo mammary epithelial cells. PPARGC1A 基因表达对水牛乳腺上皮细胞产奶量和细胞代谢的调控作用。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-24 DOI: 10.1080/10495398.2024.2344210

Seyed Mahdi Hosseini, Ye Tingzhu, Ran Zaohong, Farman Ullah, Aixin Liang, Guohua Hua, Liguo Yang

{"title":"Regulatory impacts of PPARGC1A gene expression on milk production and cellular metabolism in buffalo mammary epithelial cells.","authors":"Seyed Mahdi Hosseini, Ye Tingzhu, Ran Zaohong, Farman Ullah, Aixin Liang, Guohua Hua, Liguo Yang","doi":"10.1080/10495398.2024.2344210","DOIUrl":"10.1080/10495398.2024.2344210","url":null,"abstract":"The PPARGC1A gene plays a fundamental role in regulating cellular energy metabolism, including adaptive thermogenesis, mitochondrial biogenesis, adipogenesis, gluconeogenesis, and glucose/fatty acid metabolism. In a previous study, our group investigated seven SNPs in Mediterranean buffalo associated with milk production traits, and the current study builds on this research by exploring the regulatory influences of the PPARGC1A gene in buffalo mammary epithelial cells (BuMECs). Our findings revealed that knockdown of PPARGC1A gene expression significantly affected the growth of BuMECs, including proliferation, cell cycle, and apoptosis. Additionally, we observed downregulated triglyceride secretion after PPARGC1A knockdown. Furthermore, the critical genes related to milk production, including the STATS, BAD, P53, SREBF1, and XDH genes were upregulated after RNAi, while the FABP3 gene, was downregulated. Moreover, Silencing the PPARGC1A gene led to a significant downregulation of β-casein synthesis in BuMECs. Our study provides evidence of the importance of the PPARGC1A gene in regulating cell growth, lipid, and protein metabolism in the buffalo mammary gland. In light of our previous research, the current study underscores the potential of this gene for improving milk production efficiency and overall dairy productivity in buffalo populations.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2344210"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial-transcriptome integrative analysis of heat stress effects on amino acid metabolism and lipid peroxidation in poultry jejunum. 热应激对家禽空肠氨基酸代谢和脂质过氧化作用的微生物转录组综合分析

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-03-22 DOI: 10.1080/10495398.2024.2331179

Young-Jun Seo, Chiwoong Lim, Byeonghwi Lim, Jun-Mo Kim

{"title":"Microbial-transcriptome integrative analysis of heat stress effects on amino acid metabolism and lipid peroxidation in poultry jejunum.","authors":"Young-Jun Seo, Chiwoong Lim, Byeonghwi Lim, Jun-Mo Kim","doi":"10.1080/10495398.2024.2331179","DOIUrl":"10.1080/10495398.2024.2331179","url":null,"abstract":"Despite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host-microbiome interactions using a predicted metabolome data-based approach to understand the impact of heat stress on poultry. After the analysis, the host functional enrichment analysis revealed that pathways related to lipid and protein metabolism were elevated under heat stress conditions. In contrast, pathways related to the cell cycle were suppressed under normal environmental temperatures. In line with the transcriptome analysis, the microbial analysis results indicate that taxonomic changes affect lipid degradation. Heat stress engendered statistically significant difference in the abundance of 11 microorganisms, including Bacteroides and Peptostreptococcacea. Together, integrative approach analysis suggests that microbiota-induced metabolites affect host fatty acid peroxidation metabolism, which is correlated with the gene families of Acyl-CoA dehydrogenase long chain (ACADL), Acyl-CoA Oxidase (ACOX) and Acetyl-CoA Acyltransferase (ACAA). This integrated approach provides novel insights into heat stress problems and identifies potential biomarkers associated with heat stress.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2331179"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140189464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AMP-activated protein kinase mediates (-)-epigallocatechin-3-gallate (EGCG) to promote lipid synthesis in mastitis cows. AMP激活蛋白激酶介导(-)-表没食子儿茶素-3-棓酸盐（EGCG）促进乳腺炎奶牛的脂质合成。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-08-01 DOI: 10.1080/10495398.2024.2381080

Chunli Hu, Wenli Dou, Xuehu Ma, Yanhao An, Dezhi Wang, Yanfen Ma

{"title":"AMP-activated protein kinase mediates (-)-epigallocatechin-3-gallate (EGCG) to promote lipid synthesis in mastitis cows.","authors":"Chunli Hu, Wenli Dou, Xuehu Ma, Yanhao An, Dezhi Wang, Yanfen Ma","doi":"10.1080/10495398.2024.2381080","DOIUrl":"https://doi.org/10.1080/10495398.2024.2381080","url":null,"abstract":"Mastitis, a serious threat to the health and milk production function of dairy cows decreases milk quality. Blood from three healthy cows and three mastitis cows were collected in this study and their transcriptome was sequenced using the Illumina HiSeq platform. Differentially expressed genes (DEGs) were screened according to the |log2FoldChange| > 1 and P-value < 0.05 criteria. Pathway enrichment and functional annotation were performed through KEGG and GO analyses. Finally, the mechanism of the AMP-activated protein kinase (AMPK) mediation of (-)-epigallocatechin-3-gallate (EGCG) to promote lipid metabolism in mastitis cows was analyzed in bovine mammary epithelial cells (BMECs). Transcriptome analysis revealed a total of 825 DEGs, with 474 genes showing increased expression and 351 genes showing decreased expression. The KEGG analysis of DEGs revealed that they were mainly linked to tumour necrosis factor, nuclear factor-κB signalling pathway, and lipid metabolism-related signalling pathway, whereas GO functional annotation found that DEGs were enriched in threonine and methionine kinase activity, cellular metabolic processes, and cytoplasm. AMPK expression, which is involved in several lipid metabolism pathways, was downregulated in mastitis cows. The results of in vitro experiments showed that the inhibition of AMPK promoted the expression of lipid synthesis genes in lipopolysaccharide-induced BMECs and that EGCG could promote lipid synthesis by decreasing the expression of AMPK and downregulating the expression of inflammatory factors in inflammatory BMECs. In conclusion, our study demonstrated that AMPK mediated EGCG to inhabit of inflammatory responses and promote of lipid synthesis in inflammatory BMECs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2381080"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of stimbiotic on growth performance, nutrient digestibility, oocyst shedding, blood profiles, and intestinal microbiota in necrotic enteritis-challenged broiler. 刺激素对坏死性肠炎挑战肉鸡生长性能、营养消化率、卵囊脱落、血液特征和肠道微生物群的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-08-16 DOI: 10.1080/10495398.2024.2390936

Seyeon Chang, Woogi Kwak, Jihwan Lee, Seonwoong Kim, Dongcheol Song, Jaewoo An, Sehyun Park, Kyeongho Jeon, Hyuck Kim, Jinho Cho

{"title":"Effect of stimbiotic on growth performance, nutrient digestibility, oocyst shedding, blood profiles, and intestinal microbiota in necrotic enteritis-challenged broiler.","authors":"Seyeon Chang, Woogi Kwak, Jihwan Lee, Seonwoong Kim, Dongcheol Song, Jaewoo An, Sehyun Park, Kyeongho Jeon, Hyuck Kim, Jinho Cho","doi":"10.1080/10495398.2024.2390936","DOIUrl":"https://doi.org/10.1080/10495398.2024.2390936","url":null,"abstract":"This experiment was conducted to investigate the effect of stimbiotic (STB) in broilers with necrotic enteritis (NE). A total of 180 one-day-old Arbor Acres (initial body weight of 34.81 ± 1.04 g) were used in this experiment for 32 days. All broilers were randomly allocated into six treatments, and each experimental group had 10 replicate cages with three broilers per cage. The experiment was conducted in a 2 × 3 factorial design consisting of two levels of challenge (challenge and non-challenge) and three levels of STB (0, 0.05, and 0.1%). The NE challenge significantly decreased (P < 0.05) growth performance, heterophil levels in blood, and intestinal lesion scores compared to the non-challenge group. Supplementation of 0.05% STB significantly decreased (P < 0.05) feed conversion ratio and the number of oocysts per gram of feces compared to the supplementation of 0 and 0.1% STB. At the genus level, the supplementation of 0.05% STB significantly decreased (P < 0.05) the abundance of Enterobacterales compared to the other groups on d 32. In conclusion, supplementation with 0.05% STB in a diet could positively regulate the fecal microflora and alleviate the decline in growth performance and nutrient digestibility caused by NE.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2390936"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141992210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of genetic variants related to the fatty acid metabolic process pathway on milk production traits in Jersey cows. 与脂肪酸代谢过程途径有关的遗传变异对泽西奶牛产奶特性的影响。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI: 10.1080/10495398.2024.2396421

Sena Ardicli, Nursen Senturk, Berkay Bozkurt, Huseyn Babayev, Tuğçe Selvi, Stephen Skolnick, Hivdanur Ter, Beyza Aktas, Ayse Isık, Ozgur Toprak Ay, Ozge Ardicli, Ozden Cobanoglu

{"title":"The impact of genetic variants related to the fatty acid metabolic process pathway on milk production traits in Jersey cows.","authors":"Sena Ardicli, Nursen Senturk, Berkay Bozkurt, Huseyn Babayev, Tuğçe Selvi, Stephen Skolnick, Hivdanur Ter, Beyza Aktas, Ayse Isık, Ozgur Toprak Ay, Ozge Ardicli, Ozden Cobanoglu","doi":"10.1080/10495398.2024.2396421","DOIUrl":"10.1080/10495398.2024.2396421","url":null,"abstract":"The synthesis of fatty acids plays a critical role in shaping milk production characteristics in dairy cattle. Thus, identifying effective haplotypes within the fatty acid metabolism pathway will provide novel and robust insights into the genetics of dairy cattle. This study aimed to comprehensively examine the individual and combined impacts of fundamental genes within the fatty acid metabolic process pathway in Jersey cows. A comprehensive phenotypic dataset was compiled, considering milk production traits, to summarize a cow's productivity across three lactations. Genotyping was conducted through PCR-RFLP and Sanger sequencing, while the association between genotype and phenotype was quantified using linear mixed models. Moderate biodiversity and abundant variation suitable for haplotype analysis were observed across all examined markers. The individual effects of the FABP3, LTF and ANXA9 genes significantly influenced both milk yield and milk fat production. Additionally, this study reveals novel two-way interactions between genes in the fatty acid metabolism pathway that directly affect milk fat properties. Notably, we identified that the GGAAGG haplotype in FABP3×LTF×ANXA9 interaction may be a robust genetic marker concerning both milk fat yield and percentage. Consequently, the genotype combinations highlighted in this study serve as novel and efficient markers for assessing the fat content in cow's milk.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2396421"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction between adipocytes and macrophages participates in chick subcutaneous adipose tissue angiogenesis under cold stress conditions. 脂肪细胞和巨噬细胞之间的相互作用参与了冷应激条件下小鸡皮下脂肪组织的血管生成。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-09-17 DOI: 10.1080/10495398.2024.2400212

Yuelang Zhang,Jingxuan Li,Shanhe Wang,Mingli Wu,Haidong Zhao

{"title":"Interaction between adipocytes and macrophages participates in chick subcutaneous adipose tissue angiogenesis under cold stress conditions.","authors":"Yuelang Zhang,Jingxuan Li,Shanhe Wang,Mingli Wu,Haidong Zhao","doi":"10.1080/10495398.2024.2400212","DOIUrl":"https://doi.org/10.1080/10495398.2024.2400212","url":null,"abstract":"Previous studies have shown that subcutaneous adipose tissue is an important energy supply organ for chicks before and after birth, except yolk. So far, the significance of large deposits of subcutaneous adipose tissue in chicks is unclear. Therefore, this study takes the information interaction between adipocytes and macrophages as the starting point to explore whether adipocytes and macrophages could participate in adipose tissue fibrosis, angiogenesis, adaptive thermogenesis and other related functions in a specific metabolic environment. Under cold stress, the expression levels of genes related to lipidolysis, lipid transport and fatty acid oxidation in adipose tissue of chicks were significantly increased, but the expression levels of genes related to mitochondrial uncoupling were not significantly changed. Through Masson staining of adipose tissue of chicks under cold stress, it was found that the level of vascularization in adipose tissue of chicks was significantly increased. We found that the interaction between adipocyte and macrophage could participate in the angiogenesis related process of adipocytes in chicks through the HIF1A-VEGFA pathway. The analysis of lipid metabolism in subcutaneous adipose tissue of chicks from the perspective of cell heterogeneity will expand the understanding of lipid metabolism in chicks and provide a theoretical basis for chick rearing.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"53 1","pages":"2400212"},"PeriodicalIF":3.7,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequencing and characterization of complete mitogenome DNA of worldwide turkey (Meleagris gallopavo) populations. 全球火鸡（Meleagris gallopavo）种群完整有丝分裂基因组 DNA 的测序和特征描述。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-09-12 DOI: 10.1080/10495398.2024.2397682

Carlotta Ferrari,Stefano P Marelli,Alessandro Bagnato,Silvia Cerolini,Maria G Strillacci

{"title":"Sequencing and characterization of complete mitogenome DNA of worldwide turkey (Meleagris gallopavo) populations.","authors":"Carlotta Ferrari,Stefano P Marelli,Alessandro Bagnato,Silvia Cerolini,Maria G Strillacci","doi":"10.1080/10495398.2024.2397682","DOIUrl":"https://doi.org/10.1080/10495398.2024.2397682","url":null,"abstract":"The history of turkey (Meleagris gallopavo) domestication can be traced back to the period between 700 and 200 BC in Mexico. This process involved multiple contributors and resulted in the development of modern local turkey breeds. This research investigates the complete mitochondrial diversity across a diverse range of local turkeys. Seventy-three turkeys were sampled from various populations, including autochthonous Italian breeds, an American breed (Narragansett), as well as wild turkeys from the USA and Mexico. The mitochondrial DNA (mtDNA) was employed as a powerful tool for biodiversity and breed phylogeny investigation. An analysis of the entire mtDNA was conducted to identify breed-specific unique traits, mitochondrial-specific characteristics, and the phylogenetic relationship among turkey populations. A total of 44 polymorphic sites were identified. Brianzolo and Narragansett birds were characterized as genetically homogeneous populations. Thirty-two different haplotypes were identified when our samples were compared with mtDNA D-loop of 96 online available turkeys from various geographical countries. H1 and H2, differing for one mutation, were the most abundant, comprising 132 of the 185 sequences. H1 included samples coming from every region, while H2 was predominantly characterized by Italian samples. USA and Mexican samples appear to be more variable in their mtDNA than the other populations.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"10 1","pages":"2397682"},"PeriodicalIF":3.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142201141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of different mating ratios of broiler breeder on reproductive performance during post moult phase. 肉种鸡不同交配比例对换羽后阶段繁殖性能的影响

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-09-02 DOI: 10.1080/10495398.2024.2398707

Mohammad A Elsagheer, Omar A Mohamed, Ibrahim M Khattab, Marian K Wadea

{"title":"Impact of different mating ratios of broiler breeder on reproductive performance during post moult phase.","authors":"Mohammad A Elsagheer, Omar A Mohamed, Ibrahim M Khattab, Marian K Wadea","doi":"10.1080/10495398.2024.2398707","DOIUrl":"https://doi.org/10.1080/10495398.2024.2398707","url":null,"abstract":"Studies comparing mating ratio after forced molting are important for improving the efficiency of broiler breeder flocks. This study examined the effect of mating ratios on Egg production, chick weight, fertility, hatchability and embryonic mortality rate in Arbor Acres Plus broiler breeders post-moult phase. A total of 195 birds (62 weeks old), consisting of 15 cocks and 180 hens were randomly allocated with uniform body weight in a Completely Randomized Design into three groups based on the mating ratio (cock to hen). The groups included ratios of 1:10, 1:12, and 1:14, with each group replicated 5 times. Eggs were collected twice a day, and stored for 7 days at room temperature before placing them in the incubator. Results showed that mating ratios 1:10 and 1:12 had a positive effect on fertility rate and total hatchability compared to the 1:14 ratio. However, mating ratios did not affect laying rate, egg weight, egg mass, chick weight, hatchability of fertile eggs, and embryonic mortality rate. In conclusion, to reduce the cost of raising and caring cocks, a mating ratio of one cock to every twelve hens can be used for broiler breeders after forced molting to obtain the best fertility and hatching results.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2398707"},"PeriodicalIF":1.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

InDel mutations within the bovine PER2 gene are significantly associated with reproductive traits. 牛 PER2 基因中的 InDel 突变与繁殖性状有显著相关性。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-09-02 DOI: 10.1080/10495398.2024.2397806

Yuanzhe Yang, Jiajun Song, Siyuan Shen, Yongsheng Wang, Chuanying Pan, Jiyao Wu, Xianyong Lan

{"title":"InDel mutations within the bovine PER2 gene are significantly associated with reproductive traits.","authors":"Yuanzhe Yang, Jiajun Song, Siyuan Shen, Yongsheng Wang, Chuanying Pan, Jiyao Wu, Xianyong Lan","doi":"10.1080/10495398.2024.2397806","DOIUrl":"https://doi.org/10.1080/10495398.2024.2397806","url":null,"abstract":"Functioning as a key regulator of circadian rhythms, the PER2 gene exerts a substantial impact on the reproductive traits of animals. However, the effect of the PER2 gene on ovarian development remains unclear. In order to examine the relationship between bovine reproductive trait and the PER2 gene, a total of 901 ovarian samples were collected, categorized into different oestrus cycles (proestrus, oestrus, post-oestrus, anoestrous), and subjected to analysis for two potential insertion/deletions (InDels) in the PER2 gene. Through agarose gel electrophoresis and DNA sequencing, two polymorphic deletion mutations (P2-D5-bp, P3-D13-bp) were identified. Furthermore, a significant association between mature follicle diameter and P2-D5-bp was found (P < 0.05). Additionally, several significant correlations with ovarian length, width, height, and white body diameter were found for P3-D13-bp (P < 0.05). These findings suggested that the bovine PER2 gene plays an important role in above-mentioned reproductive traits, offering new avenues for improving cow fertility through marker-assisted selection (MAS).","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2397806"},"PeriodicalIF":1.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0