Animal Biotechnology最新文献

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Spatial distribution of maternal factors in pig mature oocytes. 猪成熟卵母细胞中母体因子的空间分布。
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-08-26 DOI: 10.1080/10495398.2024.2394692
Fuquan Zhu, Meng Yang, Dayu Wang, Yuan Jiang, Chao Jia, Yanfeng Fu, Aochen Yu, Huijun Liu, Meixia Wang, Tingzhang Wang, Honglin Liu, Juan Li
{"title":"Spatial distribution of maternal factors in pig mature oocytes.","authors":"Fuquan Zhu, Meng Yang, Dayu Wang, Yuan Jiang, Chao Jia, Yanfeng Fu, Aochen Yu, Huijun Liu, Meixia Wang, Tingzhang Wang, Honglin Liu, Juan Li","doi":"10.1080/10495398.2024.2394692","DOIUrl":"10.1080/10495398.2024.2394692","url":null,"abstract":"<p><p>It is known that asymmetrical maternal transcripts play an important role in the cell fate of the early embryo, but few studies are available in mammal oocytes especially in pig. To investigate the spatial factors in pig oocytes, the oriented bisection was established for collecting karyoplasts (NSOs) and cytoplasts (SSOs) with more than 95% efficiency. Subsequently, RNA-Seq and LC-MS/MS analysis were performed on NSOs and SSOs. Although no differentially expressed genes (DEGs) could be detected between NSOs and SSOs, 89 of the differentially expressed proteins (DEPs) were detected, that 58 proteins higher expressed but 31 proteins lower expressed in NSOs compared with SSOs. These DEPs mainly participated in the 'cell cycle' and 'ribosome' pathway, while the up-regulated DEPs were mainly GO in 'spindle' and 'positive regulation of translation', and the down-regulated DEPs were in 'cytosolic small ribosomal subunit' and 'mRNA binding'. The up-regulated DEP SIRT5 which are related to the regulation of gene expression, epigenetic were further detected and revealed. A spatial asymmetry of maternal factors at the protein level was firstly detected in pig mature oocytes.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2394692"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuron-derived neurotrophic factor promotes the differentiation of intramuscular and subcutaneous adipocytes in goat. 神经元衍生神经营养因子促进山羊肌肉和皮下脂肪细胞的分化
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-13 DOI: 10.1080/10495398.2024.2346223
An Li, Youli Wang, Ruiwen Li, Yaqiu Lin, Yanyan Li, Yong Wang, Wei Liu, Xiong Yan
{"title":"Neuron-derived neurotrophic factor promotes the differentiation of intramuscular and subcutaneous adipocytes in goat.","authors":"An Li, Youli Wang, Ruiwen Li, Yaqiu Lin, Yanyan Li, Yong Wang, Wei Liu, Xiong Yan","doi":"10.1080/10495398.2024.2346223","DOIUrl":"10.1080/10495398.2024.2346223","url":null,"abstract":"<p><p>Adipocyte play an important role in human health and meat quality by influencing the tenderness, flavor, and juiciness of mutton It has been shown that neuron-derived neurotrophic factor (<i>NENF</i>) is closely related to energy metabolism and adipocyte differentiation in bovine. However, the role of <i>NENF</i> in the goats remains unclear. The aim of this study was to detect the expression of <i>NENF</i> in goat subcutaneous and intramuscular adipocytes, temporal expression profiles of the <i>NENF</i>, and overexpressed <i>NENF</i> on the differentiation of different adipocytes. In this study, PCR amplification successfully cloned the goat <i>NENF</i> gene with a fragment length of 521 bp. In addition, the time point of highest expression of <i>NENF</i> differed between these two adipocytes differentiation processes. Overexpression of NENF in intramuscular and subcutaneous adipocytes promoted the expression levels of differentiation markers <i>CEBPβ</i> and <i>SREBP</i>, which in turn promoted the differentiation of intramuscular and subcutaneous adipocytes. This study will provide basic data for further study of the role of goats in goat adipocyte differentiation and for the final elucidation of its molecular mechanisms in regulating goat adipocyte deposition.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2346223"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian granulosa cells. β-catenin和SF1之间的协同合作调节蛋鸡卵巢颗粒细胞中孕酮的合成。
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-14 DOI: 10.1080/10495398.2024.2351975
Xueying Ma, Xu Han, Qin Zhang, Wenwen Wang, Hui Tang
{"title":"Synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian granulosa cells.","authors":"Xueying Ma, Xu Han, Qin Zhang, Wenwen Wang, Hui Tang","doi":"10.1080/10495398.2024.2351975","DOIUrl":"10.1080/10495398.2024.2351975","url":null,"abstract":"<p><p>The development of ovarian follicles in poultry is a key factor affecting the performance of egg production. Ovarian follicle development is regulated via the Wnt/β-catenin signaling pathway, and β-catenin, encoded by <i>CTNNB1,</i> is a core component of this pathway. In this study, using ovary GCs from laying hens, we investigated the regulatory role of <i>CTNNB1</i> in steroid synthesis. We found that <i>CTNNB1</i> significantly regulates the expression of <i>StAR</i> and <i>CYP11A1</i> (key genes related to progesterone synthesis) and the secretion of progesterone (P4). Furthermore, simultaneous overexpression of CTNNB1 and SF1 resulted in significantly higher levels of <i>CYP11A1</i> and secretion of P4 than in cells overexpressing CTNNB1 or SF1 alone. We also found that in GCs overexpressing <i>SF1</i>, levels of <i>CYP11A1</i> and secreted P4 were significantly greater than in controls. Silencing of <i>CYP11A1</i> resulted in the inhibition of P4 secretion while overexpression of <i>SF1</i> in <i>CYP11A1-</i>silenced cells restored P4 secretion to normal levels. Together, these results indicate that synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian hierarchical granulosa cells to promote <i>CYP11A1</i> expression.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2351975"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and characterization of biosurfactant-producing microbes isolated from the gastrointestinal system of broiler birds fed a commercial diet. 从商业日粮肉鸡胃肠系统中分离出的产生生物表面活性剂的微生物的分离和表征。
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-10-10 DOI: 10.1080/10495398.2023.2263771
Ngavaite Chigede, Zedias Chikwambi, Irvin D T Mpofu, James Madzimure
{"title":"Isolation and characterization of biosurfactant-producing microbes isolated from the gastrointestinal system of broiler birds fed a commercial diet.","authors":"Ngavaite Chigede, Zedias Chikwambi, Irvin D T Mpofu, James Madzimure","doi":"10.1080/10495398.2023.2263771","DOIUrl":"10.1080/10495398.2023.2263771","url":null,"abstract":"<p><p>Antimicrobial drug resistance (AMR) from improper use of antibiotics in various livestock products is a growing hazard for humans worldwide, with current death rate in excess of 700,000 per annum linked to the problem. Microorganisms are a rich source of structurally distinct bioactive compounds designed to protect the microbes and can offset AMR challenge. A study was conducted at Chinhoyi University of Technology to isolate, identify and characterize biosurfactant secreting microbes from broiler bird's gastrointestinal tract. Analysis of variance was performed in Genstat software. 16S rRNA technique was used to identify the DNA of isolates, annotated by similarity using BLASTn analysis against the NCBI nucleotide database. Phylogenetic analysis was performed on the BLASTn outcome to have an appreciation of the evolutionary genetic relationships. Small intestine-derived samples had a wider hemolytic activity of 5.6 mm, with a 39% emulsification index. At 98.29% sequence similarity, the bacterium producing biosurfactants was identified as an <i>Escherichia coli</i> strain similar to the 7.1994/NIST 0056 strain. The biosurfactant substance is a derivative of decane with beta lactams, tetracyclines and sulfa drugs properties which were responsible for the observed antibacterial activity. We recommend endogenous biosurfactant production optimization experiments and <i>in-vivo</i> trials to evaluate the potential impacts of a biosurfactant based feed additive in broilers.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2263771"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41181837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets. 仔猪饥饿应激诱导的小肠转录变化的 RNA-seq 分析。
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-26 DOI: 10.1080/10495398.2023.2295931
Yijia Ma, Tong Guo, Jianqin Ding, Zhiling Dong, Yifei Ren, Chang Lu, Yan Zhao, Xiaohong Guo, Guoqing Cao, Bugao Li, Pengfei Gao
{"title":"RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets.","authors":"Yijia Ma, Tong Guo, Jianqin Ding, Zhiling Dong, Yifei Ren, Chang Lu, Yan Zhao, Xiaohong Guo, Guoqing Cao, Bugao Li, Pengfei Gao","doi":"10.1080/10495398.2023.2295931","DOIUrl":"10.1080/10495398.2023.2295931","url":null,"abstract":"<p><p>Piglets may experience a variety of stress injuries, but the molecular regulatory mechanisms underlying these injuries are not well understood. In this study, we analysed the ileum of Large White (LW) and Mashen (MS) piglets at different times of starvation using chemical staining and transcriptome analysis. The intestinal barrier of piglets was damaged after starvation stress, but the intestinal antistress ability of MS piglets was stronger than LW piglets. A total of 8021 differentially expressed genes (DEGs) were identified in two breeds. Interestingly, the immune capacity (<i>CHUK</i>, <i>TLR3</i>) of MS piglets increased significantly after short-term starvation stress, while energy metabolism (<i>NAGS</i>, <i>PLA2G12B</i>, <i>AGCG8</i>) was predominant in LW piglets. After long-term starvation stress, the level of energy metabolism (<i>PLIN5</i>, <i>PLA2G12B</i>) was significantly increased in MS piglets. The expression of immune (<i>HLA-DQB1</i>, <i>IGHG4</i>, <i>COL3A1</i>, <i>CD28</i>, <i>LAT</i>) and disease (<i>HSPA1B</i>, <i>MINPPI</i>, <i>ADH1C</i>, <i>GAL3ST1</i>) related genes were significantly increased in two breeds of piglets. These results suggest that short-term stress mainly enhances immunity and energy metabolism in piglets, while long-term starvation produces greater stress on piglets, making it difficult for them to compensate for the damage to their bodies through self-regulation. This information can help improve the stress resistance of piglets through molecular breeding.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2295931"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of different mating ratios of broiler breeder on reproductive performance during post moult phase. 肉种鸡不同交配比例对换羽后阶段繁殖性能的影响
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI: 10.1080/10495398.2024.2398707
Mohammad A Elsagheer, Omar A Mohamed, Ibrahim M Khattab, Marian K Wadea
{"title":"Impact of different mating ratios of broiler breeder on reproductive performance during post moult phase.","authors":"Mohammad A Elsagheer, Omar A Mohamed, Ibrahim M Khattab, Marian K Wadea","doi":"10.1080/10495398.2024.2398707","DOIUrl":"10.1080/10495398.2024.2398707","url":null,"abstract":"<p><p>Studies comparing mating ratio after forced molting are important for improving the efficiency of broiler breeder flocks. This study examined the effect of mating ratios on Egg production, chick weight, fertility, hatchability and embryonic mortality rate in Arbor Acres Plus broiler breeders post-moult phase. A total of 195 birds (62 weeks old), consisting of 15 cocks and 180 hens were randomly allocated with uniform body weight in a Completely Randomized Design into three groups based on the mating ratio (cock to hen). The groups included ratios of 1:10, 1:12, and 1:14, with each group replicated 5 times. Eggs were collected twice a day, and stored for 7 days at room temperature before placing them in the incubator. Results showed that mating ratios 1:10 and 1:12 had a positive effect on fertility rate and total hatchability compared to the 1:14 ratio. However, mating ratios did not affect laying rate, egg weight, egg mass, chick weight, hatchability of fertile eggs, and embryonic mortality rate. In conclusion, to reduce the cost of raising and caring cocks, a mating ratio of one cock to every twelve hens can be used for broiler breeders after forced molting to obtain the best fertility and hatching results.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2398707"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Aeromonas hydrophila infection on leptin receptor overlapping transcript expression in Rana amurensis. 嗜水气单胞菌感染对金眼蛙瘦素受体重叠转录本表达的影响
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-10-14 DOI: 10.1080/10495398.2024.2410742
Yugang Sun, Tong Wu, Zhaodong Chen, Huimin Ren, Yufen Liu, Peng Liu, Wenge Zhao
{"title":"Effect of <i>Aeromonas hydrophila</i> infection on leptin receptor overlapping transcript expression in <i>Rana amurensis</i>.","authors":"Yugang Sun, Tong Wu, Zhaodong Chen, Huimin Ren, Yufen Liu, Peng Liu, Wenge Zhao","doi":"10.1080/10495398.2024.2410742","DOIUrl":"10.1080/10495398.2024.2410742","url":null,"abstract":"<p><p>The leptin receptor overlapping transcript (LepROT) has been suggested to play several roles in immunomodulatory mechanisms; however, the understanding of its role in <i>Rana amurensis</i> immunity is still very limited. Here, we performed hematoxylin-eosin staining, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), immunofluorescence and western blotting to investigate the roles of LepROT in the immunomodulatory mechanism and the influence of its expression on the nuclear factor-kappa B (NF-κB) signaling pathway, such as the activation of IκB kinase and NF-кB, in amphibian resistance to infection with <i>Aeromonas hydrophila</i> (<i>Ah</i>). After <i>Ah</i> infection, the liver, lung, kidney, skin, muscle, and stomach of <i>R. amurensis</i> showed cell structure disturbance, bleeding, and texture abnormalities. In addition, the relative expression levels of LepROT, NF-кB, IKKα, and IKKβ were all upregulated after <i>Ah</i> infection; however, they showed time-dependent differential expression. The NF-кB signaling pathway exhibited robust expression levels, which might be explained by the positive feedback regulation function of LepROT. Overall, this study provides a basis for further assessment of the biological functions of LepROT and highlights its role in the regulation of immune mechanisms.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2410742"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype. 甲状腺转录组图谱揭示了与FecB BB基因型小尾寒羊多产有关的lncRNA和mRNA的不同调控。
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-02-29 DOI: 10.1080/10495398.2024.2312393
Cheng Chang, Xiaoyun He, Ran Di, Xiangyu Wang, Miaoceng Han, Chen Liang, Mingxing Chu
{"title":"Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with <i>FecB BB</i> genotype.","authors":"Cheng Chang, Xiaoyun He, Ran Di, Xiangyu Wang, Miaoceng Han, Chen Liang, Mingxing Chu","doi":"10.1080/10495398.2024.2312393","DOIUrl":"10.1080/10495398.2024.2312393","url":null,"abstract":"<p><strong>Background: </strong>The thyroid gland is an important endocrine gland in animals that secretes thyroid hormones and acts on various organs throughout the body. lncRNAs are long non-coding RNAs that play an important role in animal reproduction; however, there is a lack of understanding of their expression patterns and potential roles in the thyroid gland of the Small Tail Han (STH) sheep. In this study, we used RNA-Seq technology to examine the transcriptome expression pattern of the thyroid from the luteal phase (LP) and follicular phase (FP) of FecB BB (MM) STH sheep.</p><p><strong>Results: </strong>We identified a total of 122 and 1287 differential expression lncRNAs (DELs) and differential expression mRNAs (DEGs), respectively, which were significantly differentially expressed. These DELs target genes and DEGs can be enriched in several signalling pathways related to the animal reproduction process.</p><p><strong>Conclusions: </strong>The expression profiles of DELs and DEGs in thyroid glands provide a more comprehensive resource for elucidating the reproductive regulatory mechanisms of STH sheep.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2312393"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of forages on the microbiota of crossed sheep on cold Plateau. 饲草对寒带高原杂交绵羊微生物群的影响
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-06-10 DOI: 10.1080/10495398.2024.2362639
Yue Ren, Renzeng Ciwang, Khalid Mehmood, Kun Li
{"title":"Effects of forages on the microbiota of crossed sheep on cold Plateau.","authors":"Yue Ren, Renzeng Ciwang, Khalid Mehmood, Kun Li","doi":"10.1080/10495398.2024.2362639","DOIUrl":"10.1080/10495398.2024.2362639","url":null,"abstract":"<p><p>Diet is an important component to influence microbiota, there are less data available about the microbiome of Suffolk cross with Tibetan (SCT) animals with different fodders. The current study was conducted for comparing the fungi microbiota in SCT sheep fed with different forages. Sequencing of ileum samples from sheep groups of AH (alfalfa and oat grass), BH (mixture of grass and concentrated feeds), CH (concentrated feed I), DH (concentrated feed II) and EH (concentrated feed III) achieved 3,171,271 raw and 2,719,649 filtered sequences. Concentrated feeds changed fungi microbiota in SCT sheep with three phyla and 47 genera significantly different among the groups. Genera include positive genus of <i>Scytalidium</i> and negative fungi of <i>Sarocladium</i>, <i>Kazachstania</i>, <i>Gibberella</i>, <i>Scytalidium</i>, <i>Candida</i>, <i>Wickerhamomyces</i>. The findings of our study will contribute to efficient feeding of SCT sheep at cold plateau areas.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2362639"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Core promoter identification and transcriptional regulation of porcine ACSL3 gene. 猪 ACSL3 基因的核心启动子识别和转录调控。
IF 1.8 3区 农林科学
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-11-25 DOI: 10.1080/10495398.2024.2430383
Xiaomin Li, Zijiao Guo, Xueying Ma, Huixin Liu, Wenwen Wang, Hui Tang
{"title":"Core promoter identification and transcriptional regulation of porcine <i>ACSL3</i> gene.","authors":"Xiaomin Li, Zijiao Guo, Xueying Ma, Huixin Liu, Wenwen Wang, Hui Tang","doi":"10.1080/10495398.2024.2430383","DOIUrl":"10.1080/10495398.2024.2430383","url":null,"abstract":"<p><p>Intramuscular fat (IMF) content is an important factor that affects the edible and processing quality of pork. Studying the transcriptional regulation mechanisms of genes affecting intramuscular fat deposition can provide theoretical support for genetic improvement in pigs. Long-chain fatty acyl-CoA synthase 3 (<i>ACSL3</i>), as a key enzyme in the process of lipid synthesis in mammals. However, no information about the core promoter of the <i>ACSL3</i> gene and its transcriptional regulation has been reported so far. In this experiment, we successfully cloned 3112 bp of the porcine <i>ACSL3</i> gene promoter region. In order to find out the core promoter of the <i>ACSL3</i> gene. The results indicated that the core promoter region of the <i>ACSL3</i> gene is located from -111 bp to -59 bp upstream of the transcription initiation site (TSS). To identify the interaction between SP1 and the <i>ACSL3</i> gene promoter, we mutated the predicted binding sites of <i>ACSL3</i> gene promoter. The results showed that the activity of the promoter was decreased by site-specific mutagenesis of the SP1 transcription factor binding site, while overexpression of SP1 increased the expression of the <i>ACSL3</i> gene. In summary, our study identified a core promoter region of the porcine <i>ACSL3</i> gene, and the SP1 binding site is responsible for the promoter activity.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2430383"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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