Animal Biotechnology最新文献

{"title":"Gene co-expression network and differential expression analyses reveal key genes for weaning weight in Simmental-Holstein crossbred cattle.","authors":"Saina Yan, Fen Pei, Jingfnag Si, Md Yousuf Ali Khan, Sihai Ou, Yang Yang, Zongsheng Zhao, Alfredo Pauciullo, Yi Zhang","doi":"10.1080/10495398.2024.2404042","DOIUrl":"https://doi.org/10.1080/10495398.2024.2404042","url":null,"abstract":"<p><p>Weaning weight is a key indicator of the early growth performance of cattle. An understanding of the genetic mechanisms underlying weaning weight will help increase the accuracy of selection of breeding animals. In order to identify candidate genes associated with weaning weight in Simmental-Holstein crossbred cattle, this study generated RNA-Sequencing (RNA-seq) data from 86 crossbred calves (37 males and 49 famales) and measured their weaning weight and body size traits (wither height, body length, chest girth, rump width, and rump length). Differential gene expression analysis and weighted gene co-expression network analysis (WGCNA) were performed. A total of 498 differentially expressed genes (DEGs) were identified between the low weaning weight (LWW) group and the high weaning weight (HWW) group. Weaning weight was transcriptionally correlated (FDR < 0.05) with four of the eleven co-expression gene modules. By intersecting DEGs and hub genes of the four modules, we identified a final set of 37 candidate genes enriched in growth, development, or immune-related processes. In addition, one co-expression module was significantly correlated with all the five body size traits (P < 0.05), from which <i>MX1</i> was identified as a key candidate gene through protein-protein interaction (PPI) analysis of hub genes. Further evidence from cattle transcriptome-wide association study analysis (TWAS) and human phenome-wide association study (PheWAS) validated significant associations of <i>CACNA1S</i>, <i>SEMA7A</i>, <i>VCAN</i>, <i>CD101</i>, <i>CD19</i>, and <i>CSF2RB</i> with growth and development traits (P < 0.05). Notably, <i>CACNA1S</i> and <i>CD19</i> were also associated with typical immune traits such as B cell proliferation, differentiation, and activation. In conclusion, this study reveals new candidate genes significantly associated with weaning weight in Simmental-Holstein crossbred cattle, providing a basis for further exploration of the genetic mechanisms behind growth traits of cattle.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2404042"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full-length 16S rRNA gene amplicon analysis of gut microbiota in pigs fed with different diets in growing and finishing stages.","authors":"Han-Sheng Wang, Sra-Yh Shih, Yu-Ling Huang, Chia-Chieh Chang, HsinYuan Tsai","doi":"10.1080/10495398.2024.2414299","DOIUrl":"https://doi.org/10.1080/10495398.2024.2414299","url":null,"abstract":"<p><p>The present study utilized full-length 16S rRNA gene sequencing to investigate the impact of dietary protein content on the composition and function of gut microbiota, and to analyze the gut microbiota of pigs in the growing (30 kg) and finishing (120 kg) stages under different feeding conditions. The results indicated that the gut microbiota was significantly different between pigs fed high- and low-protein diets. Comparing fecal samples from pigs at 30 and 120 kg, pigs at 30 kg showed a significant increase in the relative abundance of <i>Clostridium butyricum</i>, whereas at 120 kg, the abundance of <i>Lactobacillus reuteri</i> and <i>Lactobacillus johnsonii</i> decreased. To access the functional profiles and metabolic pathways based on amplicon sequence variants (ASVs), the microbiome of the 120 kg exhibited significant enrichments in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to metabolism-related category, including Alanine, aspartate and glutamate metabolism, Tyrosine and Thiamin metabolism, and Inositol phosphate metabolism. Meanwhile, analysis using the MetaCyc database showed that the metabolic pathways of the 30 kg group were significantly distinct when compared to the 120 kg of fecal samples. Overall, the findings indicated that the gut microbiota composition and function in the 30 and 120 kg fecal samples were markedly shaped by different dietary protein levels.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2414299"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of genomic regions affecting nitrogen excretion intensity in Brown Swiss dairy cows.","authors":"Žan Pečnik, Daša Jevšinek Skok","doi":"10.1080/10495398.2024.2434097","DOIUrl":"https://doi.org/10.1080/10495398.2024.2434097","url":null,"abstract":"<p><p>Dairy cows with a lower nitrogen excretion intensity (N_exi) excrete less nitrogen, ammonia (NH₃) and nitrous oxide (N₂O), a highly potent greenhouse gas (GHG), per kg of milk produced and therefore represent a lower environmental impact while maintaining food security. To date, the genomics background of N_exi is unknown. Here we performed a genetic association study, overlap analysis and functional enrichment analysis for N_exi in 875 genotyped dairy cows with 2,147 lactations from 200 herds. We identified 1456 single nucleotide polymorphisms (SNPs) that significantly affect N_exi. We found 140 SNPs overlapping with 148 protein-coding genes. The <i>MAN1A1</i> gene is a strong candidate gene for N_exi. Genotype CC of rs42786248, the most significantly associated SNP located in the <i>MAN1A1</i> gene, had higher N_exi than genotypes AA (<i>p</i> < 0.001) and AC (<i>p</i> < 0.001). We identified 33 genes involved in biological processes related to nitrogen metabolism. Our results form the basis for further research on the genomics background of N_exi. The identified SNPs serve as potential targets for selective breeding programs, aimed at reducing N_exi and associated NH₃ and N₂O emissions in cattle production, thus contributing to more environmentally sustainable milk production.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2434097"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discover QTLs for the level of blood components in Shaoxing duck using GWAS and haplotype sharing analysis.","authors":"Wenwu Xu, Zhenzhen Wang, Zeng Tao, Kui Li, Lizhi Lu","doi":"10.1080/10495398.2024.2390940","DOIUrl":"https://doi.org/10.1080/10495398.2024.2390940","url":null,"abstract":"<p><p>Blood composition is indicative of health-related traits such as immunity and metabolism. The use of molecular genetics to investigate alterations in these attributes in laying ducks is a novel approach. Our objective was to employ genome - wide association studies (GWAS) and haplotype - sharing analysis to identify genomic regions and potential genes associated with 11 blood components in Shaoxing ducks. Our findings revealed 35 SNPs and 1 SNP associated with low-density lipoprotein cholesterol (LDL) and globulin (GLB), respectively. We identified 36 putative candidate genes for the LDL trait in close proximity to major QTLs and key loci. Based on their biochemical and physiological properties, <i>TRA2A</i>, <i>NPY</i>, <i>ARHGEF26</i>, <i>DHX36</i>, and <i>AADAC</i> are the strongest putative candidate genes. Through linkage disequilibrium analysis and haplotype sharing analysis, we identified three haplotypes and one haplotype, respectively, that were significantly linked with LDL and GLB. These haplotypes could be selected as potential candidate haplotypes for molecular breeding of Shaoxing ducks. Additionally, we utilized a bootstrap test to verify the reliability of GWAS with small experimental samples. The test can be accessed at https://github.com/xuwenwu24/Bootstrap-test.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2390940"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin C enhances the <i>in vitro</i> development of early porcine embryos by improving mitochondrial function.","authors":"Lei Wang, Liu She, Peng Qiu, Meiyun Lv, Yunchuan Zhang, Yunjia Qi, Qin Han, Deshun Shi, Chan Luo","doi":"10.1080/10495398.2024.2404043","DOIUrl":"https://doi.org/10.1080/10495398.2024.2404043","url":null,"abstract":"<p><p>Mammalian embryos often suffer from oxidative stress in vitro, as the oxygen in the atmosphere is higher than that in the oviductal environment. Vitamin C (Vc) has been proven to enhance early embryonic development <i>in vitro</i>, but the underlying mechanism remains unclear. In this study, we investigated the pathways of action by which Vc promotes the <i>in vitro</i> development of porcine embryos. Comparative analysis of <i>in vitro</i> and <i>in vivo</i> gene expression profiles of morula found that most of the differentially expressed genes were enriched in pathways related to mitochondrial function. The addition of 12.5 μg/mL Vc to the culture medium significantly increased blastocyst production in a dose- and duration-dependent manner. Moreover, ROS levels were significantly higher in embryos cultured in the air (21% oxygen) than cultured in a hypoxic condition (5% oxygen) and were reduced by Vc supplementation. Vc also significantly increased the mitochondrial membrane potential levels and the expression levels of mitochondrial function-related genes (<i>MFN1</i> and <i>OPA1</i>) and TCA cycle-related genes (<i>PDHA1</i> and <i>OGDH</i>) in embryos cultured <i>in vitro</i>. These results suggest that the addition of Vc to the <i>in vitro</i> culture medium can increase the developmental potential and improve the mitochondrial function of early porcine embryos.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2404043"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary ginger (<i>Zingiber officinale</i>) enhances performance traits, biochemical and haematological indices of Turkey targeting mRNA gene expression.","authors":"Eman A Manaa, Mervat A Abdel-Latif, Samya E Ibraheim, Abdelaziz M Sakr, Hanaa M Ghanem, Rania M Waheed, Ghadeer M Albadrani, Mohamed M Abdel-Daim, Amr El Zawily, Basant M Shafik","doi":"10.1080/10495398.2024.2425656","DOIUrl":"https://doi.org/10.1080/10495398.2024.2425656","url":null,"abstract":"<p><p>Ginger rich in polyphenols, possesses various biomedical properties. Researchers investigated the effects of dietary ginger supplementation on turkey performance traits, biochemical parameters, haematological parameters and mRNA gene expression. Ginger root powder was administered at different doses (0, 10, 20 and 40 g/kg) to the turkeys. Notably, the 20 g/kg group exhibited improved performance traits and a higher broiler production efficiency factor (BPEF). Importantly, ginger was found to be safe for turkeys based on serum indices. Furthermore, the expression of several growth-related genes, including growth hormone receptor (GHR), insulin-like growth factor 1 (IGF-1), adenine nucleotide translocase (ANT), cyclooxygenase 3 (COX-3) and uncoupling protein 3 (UCP-3), was upregulated in the 20 g/kg enhancing their growth performance and economic efficiency in addition to keeping their health status safe. Therefore, Ginger root powder can be supplemented for turkey at a concentration of 2% as the addition of ginger powder is a long-term process.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2425656"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus.","authors":"Chongfa Yang, Yahua Yang, Bingzhu Zhao, Enyu Gao, Hao Chen, Yang Li, Junyuan Ma, Jine Wang, Songming Hu, Xiaochen Song, Ying Chen, Gengsacairang Yang, Shengdong Huo, Wenxue Luo","doi":"10.1080/10495398.2024.2427757","DOIUrl":"10.1080/10495398.2024.2427757","url":null,"abstract":"<p><p>Since most yaks have a long postpartum anestrus period, postpartum anestrus is the main factor affecting the reproductive efficiency of yaks. In this study, the third-generation sequencing technology was used to successfully screen differentially expressed genes (DEGs) and differentially expressed transcripts (DETs) in the ovarian tissues of yaks during estrus and anestrus. The functional references of DEGs and DETs were Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Clusters of Orthologous Genes database. A total of 1149 DEGs and 2294 DETs were successfully identified. These DEGs and DETs were mainly related to biological processes such as \"reproduction\", \"reproductive process\", \"metabolic process\" and \"rhythmic process\". Kisspeptin-G protein-coupled receptor was found to be involved in regulating the reproductive cycle of yaks. DEGs and DETs were also related to gonadotropin-releasing hormone (GnRH) signaling pathways such as oocyte meiosis, estrogen signaling pathway, and progesterone-mediated induced oocyte maturation. The results showed that <i>SIRT1</i>, <i>CSNK1A1</i>, <i>SLIT3</i>, <i>INHBA</i>, <i>INSL3</i>, <i>ZP2</i>, <i>Clock</i>, <i>BMP15</i>, <i>Bmal1</i>, <i>KISS1</i>, and <i>LCHGR</i> regulate the postpartum quiescent state and the reproductive cycle of yaks. This study will help to further clarify the reproductive mechanism of yaks at the molecular level and provide certain assistance for the development of animal husbandry.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2427757"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring aptamers for targeted enrichment of X sperm in bovine: unraveling selective potential.","authors":"Sumit Kumar Singh, Rohit Kumar, Manya Mathur, Himanshu Kamboj, Jai Kumar Kaushik, Ashok Kumar Mohanty, Sudarshan Kumar","doi":"10.1080/10495398.2024.2323592","DOIUrl":"https://doi.org/10.1080/10495398.2024.2323592","url":null,"abstract":"<p><p>Nucleic acid aptamers have been used in the past for the development of diagnostic methods against a number of targets such as bacteria, pesticides, cancer cells etc. In the present study, six rounds of Cell-SELEX were performed on a ssDNA aptamer library against X-enriched sperm cells from Sahiwal breed cattle. Sequencing was used to examine the aptamer sequences that shown affinity for sperm carrying the X chromosome in order to find any possible X-sperm-specific sequences. Out of 35 identified sequences, 14 were selected based on bioinformatics analysis like G-Score and Mfold structures. Further validation of their specificity was done via fluorescence microscopy. The interaction of biotinylated-aptamer with sperm was also determined by visualizing the binding of streptavidin coated magnetic beads on the head region of the sperm under bright field microscopy. Finally, a real-time experiment was designed for the validation of X-sperm enrichment by synthesized aptamer sequences. Among the studied sequences, aptamer 29a exhibited a higher affinity for X sperm compared to Y sperm in a mixed population of sperm cells. By using aptamer sequence 29a, we obtained an enrichment of 70% for X chromosome bearing sperm cells.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2323592"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141069584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular cloning of <i>TPM3</i> gene in qinchuan cattle and its effect on myoblast proliferation and differentiation.","authors":"Juntao Guo, Jianfang Wang, Ke Zhang, Zhimei Yang, Bingzhi Li, Yueting Pan, Hengwei Yu, Shengchen Yu, Sayed Haidar Abbas Raza, Belete Kuraz Abebea, Linsen Zan","doi":"10.1080/10495398.2024.2345238","DOIUrl":"10.1080/10495398.2024.2345238","url":null,"abstract":"<p><p>Tropomyosin 3 (<i>TPM3</i>) plays a significant role as a regulatory protein in muscle contraction, affecting the growth and development of skeletal muscles. Despite its importance, limited research has been conducted to investigate the influence of <i>TPM3</i> on bovine skeletal muscle development. Therefore, this study revealed the role of <i>TPM3</i> in bovine myoblast growth and development. This research involved conducting a thorough examination of the Qinchuan cattle <i>TPM3</i> gene using bioinformatics tools to examine its sequence and structural characteristics. Furthermore, <i>TPM3</i> expression was evaluated in various bovine tissues and cells using quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that the coding region of <i>TPM3</i> spans 855 bp, with the 161st base being the T base, encoding a protein with 284 amino acids and 19 phosphorylation sites. This protein demonstrated high conservation across species while displaying a predominant α-helix secondary structure despite being an unstable acidic protein. Notably, a noticeable increase in <i>TPM3</i> expression was observed in the longissimus dorsi muscle and myocardium of calves and adult cattle. Expression patterns varied during different stages of myoblast differentiation. Functional studies that involved interference with <i>TPM3</i> in Qinchuan cattle myoblasts revealed a very significantly decrease in S-phase cell numbers and EdU-positive staining (<i>P</i> < 0.01), and disrupted myotube morphology. Moreover, interference with <i>TPM3</i> resulted in significantly (<i>P</i> < 0.05) or highly significantly (<i>P</i> < 0.01) decreased mRNA and protein levels of key proliferation and differentiation markers, indicating its role in the modulation of myoblast behavior. These findings suggest that <i>TPM3</i> plays an essential role in bovine skeletal muscle growth by influencing myoblast proliferation and differentiation. This study provides a foundation for further exploration into the mechanisms underlying <i>TPM3</i>-mediated regulation of bovine muscle development and provides valuable insights that could guide future research directions as well as potential applications for livestock breeding and addressing muscle-related disorders.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2345238"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of miR-23b-3p on regulating GH by targeting POU1F1 in Yanbian yellow cattle.","authors":"Lu Xu, Taihua Jin, Angang Lou, Jiuyang Guan, Xinglin Zhang, Hui Wang, Lizeng Guan","doi":"10.1080/10495398.2024.2346808","DOIUrl":"10.1080/10495398.2024.2346808","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of miR-23b-3p on growth hormone (GH) in pituitary cells of Yanbian yellow cattle. The mRNA and protein levels of GH and miR-23b-3p target genes were measured by real time fluorescence quantitative PCR (qPCR) and Western blot, respectively. The target relationship of miR-23b-3p was validated by double luciferase reporter gene system. The results showed that GH mRNA and protein levels in pituitary cells of Yanbian yellow cattle were significantly lower in the miR-23b-3p-mi group than in the NC group (<i>P</i><0.01), while GH mRNA and protein levels were higher in the miR-23b-3p-in group than in the iNC group (<i>P</i><0.05). The result of bioinformatics analysis and double luciferase reporter gene system validation proved that miR-23b-3p targeted 3'UTR of pituitary specific transcription factor 1 (POU1F1). POU1F1 mRNA and protein levels were lower miR-23b-3p-mi group than in the NC group (<i>P</i><0.01), while POU1F1 mRNA and protein levels were higher in the miR-23b-3p-in group than in the iNC group (<i>P</i><0.01). These results demonstrated that miR-23b-3p could regulate GH expression in pituitary cells by regulating <i>POU1F1</i> gene.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2346808"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}