Animal Biotechnology最新文献

{"title":"Discover QTLs for the level of blood components in Shaoxing duck using GWAS and haplotype sharing analysis.","authors":"Wenwu Xu, Zhenzhen Wang, Zeng Tao, Kui Li, Lizhi Lu","doi":"10.1080/10495398.2024.2390940","DOIUrl":"https://doi.org/10.1080/10495398.2024.2390940","url":null,"abstract":"<p><p>Blood composition is indicative of health-related traits such as immunity and metabolism. The use of molecular genetics to investigate alterations in these attributes in laying ducks is a novel approach. Our objective was to employ genome - wide association studies (GWAS) and haplotype - sharing analysis to identify genomic regions and potential genes associated with 11 blood components in Shaoxing ducks. Our findings revealed 35 SNPs and 1 SNP associated with low-density lipoprotein cholesterol (LDL) and globulin (GLB), respectively. We identified 36 putative candidate genes for the LDL trait in close proximity to major QTLs and key loci. Based on their biochemical and physiological properties, <i>TRA2A</i>, <i>NPY</i>, <i>ARHGEF26</i>, <i>DHX36</i>, and <i>AADAC</i> are the strongest putative candidate genes. Through linkage disequilibrium analysis and haplotype sharing analysis, we identified three haplotypes and one haplotype, respectively, that were significantly linked with LDL and GLB. These haplotypes could be selected as potential candidate haplotypes for molecular breeding of Shaoxing ducks. Additionally, we utilized a bootstrap test to verify the reliability of GWAS with small experimental samples. The test can be accessed at https://github.com/xuwenwu24/Bootstrap-test.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2390940"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary investigation reveals novel pathological consequences of bluetongue virus-1 infection in the endocrine glands of pregnant Indian sheep.","authors":"Rohit Singh, Karam Pal Singh, Rajendra Singh, Vidya Singh, Pawan Kumar, Rajat Varshney, Akanksha Yadav, Akash Mote, Mukesh Gangwar, N Babu Prasath","doi":"10.1080/10495398.2023.2269428","DOIUrl":"10.1080/10495398.2023.2269428","url":null,"abstract":"<p><p>Bluetongue virus (BTV), a major peril to the sheep industry, infects a wide range of the cells in the infected animals including mononuclear, dendritic and epithelial cells. However, little is known about its tropism for the secretory epithelial cells of endocrine glands and the pathogenesis it induces. The aim of the study was to assess the BTV load, antigen distribution in the tissue of the pituitary, thyroid as well as adrenal glands and associated histopathological consequences. BTV antigens were localized using immunohistochemistry in the thyroid's epithelial cells, zona fasciculata and zona reticularis cells and the anterior pituitary epithelial cells. The real-time PCR portrayed the high viral load in adrenals at 7^th days postinoculation (DPI) and in thyroid and pituitary glands at 15^th DPI. Serum examination revealed variation in the T-3 and T-4 of infected animals in comparison to the control group. Caspase-3 immunolocalization revealed BTV-1 induces apoptosis in the affected cells of endocrine gland of infected animals. Further, this study signifies the tropism of BTV in the novel sites (endocrine glands) of the host that might be one of the reasons for the poor performance of infected animals.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2269428"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41231836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of the <i>GPAT4</i> gene mutation predicted to affect splicing.","authors":"Sergey A Bursakov, Anastasia V Kovaleva, Artyom V Brigida, Oleg G Zaripov","doi":"10.1080/10495398.2023.2269210","DOIUrl":"10.1080/10495398.2023.2269210","url":null,"abstract":"<p><p>The <i>GPAT4</i> gene is considered as a potential functional candidate for single nucleotide polymorphism (SNP) studies in dairy cattle breeding due to its association with dairy performance in cattle by encoding an enzyme responsible for the presence of diacylglycerols and triacylglycerols in milk. Using the example of the <i>GPAT4</i> gene, we applied the minigene splicing assay to analyze the functional consequences of its variant that was predicted to affect normal splicing. The results of functional analysis revealed the sequence variations (rs442541537), transfection experiments in a wild type and mutant cell line model system demonstrated that the investigated mutation in the second intron of the <i>GPAT4</i> gene was responsible for the presence of a second exon in mature messenger RNA (mRNA). The cases of its absence in the spliced mature mRNA transcript resulted in a truncated dysfunctional protein due to the appearance of a stop codon. Thus, the discovered SNP led to alternative splicing in pre-mRNA by the 'cassette exon' ('exon skipping') mechanism. The studied mutation can potentially be a molecular genetic marker for alternative splicing for the <i>GPAT4</i> gene and, therefore contributes to economic benefits in cattle breeding programs.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2269210"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71419925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size.","authors":"Mònica Ferrer-Roda, Ana Gil, Maria-Teresa Paramio, Dolors Izquierdo","doi":"10.1080/10495398.2024.2422316","DOIUrl":"https://doi.org/10.1080/10495398.2024.2422316","url":null,"abstract":"<p><p>Oocytes spontaneously resume meiosis following their liberation from follicles, preventing full competence acquisition. Biphasic IVM (CAPA-IVM) maintains oocytes in meiotic arrest to improve developmental competence, and it specially affects poorly developed oocytes. We assessed the effect of CAPA-IVM on oocytes from small (<3mm) and large (>3mm) follicles of prepubertal goats. Oocytes were cultured for 6h in pre-IVM with C-type natriuretic peptide (CNP) and estradiol as meiotic inhibitors, and germinal vesicle (GV) rate and chromatin configuration were assessed. Then, oocytes were cultured in conventional IVM (c-IVM) or CAPA-IVM (pre-IVM + c-IVM) and EGF receptor (EGFR) protein expression, intra-oocyte ROS and blastocyst development were assessed. GV rate was higher in CNP groups than control (69% vs 28%, and 67% vs 31%, small and large follicles, respectively), but GV chromatin configuration was similar. In large follicles, EGFR expression was higher in oocytes and cumulus cells after CAPA-IVM, and ROS levels were lower. In small follicles these differences were not observed. c-IVM and CAPA-IVM produced similar blastocyst rates in small (3.7% vs 2.6%, respectively) and large follicles (8.3% vs 2.5%). Overall, CAPA-IVM enhanced EGFR expression for EGF peptide signalling and antioxidant capacity in oocytes from large follicles but oocytes from small follicles were too immature to benefit from it.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2422316"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-tissue gene expression profiling of cows with a genetic predisposition for low and high milk urea levels.","authors":"Henry Reyer, Hanne Honerlagen, Michael Oster, Siriluck Ponsuksili, Björn Kuhla, Klaus Wimmers","doi":"10.1080/10495398.2024.2322542","DOIUrl":"10.1080/10495398.2024.2322542","url":null,"abstract":"<p><p>Milk urea (MU) concentration is proposed as an indicator trait for breeding toward reduced nitrogen (N) emissions and leaching in dairy. We selected 20 German Holstein cows based on MU breeding values, with 10 cows each having low (LMUg) and high (HMUg) MU genetic predisposition. Using RNA-seq, we characterized these cows to unravel molecular pathways governing post-absorptive body N pools focusing on renal filtration and reabsorption of nitrogenous compounds, hepatic urea formation and mammary gland N excretion. While we observed minor adjustments in cellular energy metabolism in different tissues associated with different MU levels, no transcriptional differences in liver ammonia detoxification were detected, despite significant differences in MU between the groups. Differential expression of <i>AQP3</i> and <i>SLC38A2</i> in the kidney provides evidence for higher urea concentration in the collecting duct of LMU cows than HMU cows. The mammary gland exhibited the most significant differences, particularly in tricarboxylic acid (TCA) cycle genes, amino acid transport, tRNA binding, and casein synthesis. These findings suggest that selecting for lower MU could lead to altered urinary urea (UU) handling and changes in milk protein synthesis. However, given the genetic variability in N metabolism components, the long-term effectiveness of MU-based selection in reducing N emissions remains uncertain.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2322542"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139995296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of OCT4 and PGP9.5 gene expression in prenatal and postnatal buffalo (<i>Bubalus bubalis</i>) testes.","authors":"Ashritha Q Carlo, Devendra Pathak, Ratan K Choudhary, Opinder Singh, Neelam Bansal","doi":"10.1080/10495398.2023.2285509","DOIUrl":"10.1080/10495398.2023.2285509","url":null,"abstract":"<p><p>This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of <i>OCT4</i> and <i>PGP9.5</i>. The relative expression of the <i>OCT4</i> gene was down-regulated in the adult testes compared to its expression during prepubertal and neonatal life. The relative expression of the <i>PGP9.5</i> gene was up-regulated in the neonatal testes and down-regulated in the prepubertal and adult testes. The spermatogonia were round, oval-to-ellipsoidal cells lying over the basement membrane (BM) with a round-to-oval nucleus. Based on the immunoexpression of the putative SSC markers, OCT4 and PGP9.5, we concluded that the proportion of stem cells was highest during the neonatal stage, followed by the prepubertal and prenatal stages. This finding sheds light on the dynamics of spermatogonial stem cells in buffalo testes at different developmental stages, providing valuable insights into these cells' regulation and potential applications.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2285509"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138440222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of <i>AGO2</i> and <i>PLEC</i> genes polymorphisms in Hu sheep and their relationship with body size traits.","authors":"Jia Liu, Wenxin Zheng, Weimin Wang, Xiaobin Yang, Yongliang Huang, Panpan Cui, Zongwu Ma, Xiwen Zeng, Rui Zhai, Xiuxiu Weng, Weiwei Wu, Xiaoxue Zhang","doi":"10.1080/10495398.2023.2295926","DOIUrl":"10.1080/10495398.2023.2295926","url":null,"abstract":"<p><p>The body size traits are major traits in livestock, which intuitively displays the development of the animal's bones and muscles. This study used PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR) to analyze the Single-nucleotide polymorphism and expression characteristics of Argonaute RISC catalytic component 2 (<i>AGO2</i>) and Plectin (<i>PLEC</i>) genes in Hu sheep. Two intron mutations were found in Hu sheep, which were <i>AGO2</i> g.51700 A > C and <i>PLEC</i> g.23157 C > T, respectively. Through association analysis of two mutation sites and body size traits, it was found that <i>AGO2</i> g.51700 A > C mainly affects the chest and cannon circumference of Hu sheep of while <i>PLEC</i> g.23157 C mainly affects body height and body length. The combined genotypes of <i>AGO2</i> and <i>PLEC</i> genes with body size traits showed SNPs at the <i>AGO2</i> g.51700 A > C and <i>PLEC</i> g.23157 C > T loci significantly improved the body size traits of Hu sheep. In addition, the <i>AGO2</i> gene has the highest expression levels in the heart, rumen, and tail fat, and the <i>PLEC</i> gene is highly expressed in the heart. These two loci can provide new research ideas for improving the body size traits of Hu sheep.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2295926"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of selenium-enriched yeast dietary supplementation on egg quality, gut morphology and caecal microflora of laying hens.","authors":"Ruili Li, Jiewei Liu, Minxiao Liu, Mingzhi Liang, Zengguang Wang, Yufen Sha, Huiwen Ma, Yafeng Lin, Baohua Li, Jinming You, Lei Zhang, Ming Qin","doi":"10.1080/10495398.2023.2258188","DOIUrl":"10.1080/10495398.2023.2258188","url":null,"abstract":"<p><p>Selenium (Se) is an essential micronutrient for humans and animals and is a powerful antioxidant that can promote reproductive and immune functions. The purpose of this study was to evaluate the effects of supplemental dietary selenium-enriched yeast (SeY) on egg quality, gut morphology and microflora in laying hens. In total, 100 HY-Line Brown laying hens (45-week old) were randomly allocated to two groups with 10 replicates and fed either a basal diet (without Se supplementation) or a basal diet containing 0.2 mg/kg Se in the form of SeY for 8 weeks. The Se supplementation did not have a significant effect on egg quality and intestinal morphology of laying hens. Based on the 16S rRNA sequencing, SeY dietary supplementation effectively modulated the cecal microbiota structure. An alpha diversity analysis demonstrated that birds fed 100 mg/kg SeY had a higher cecal bacterial diversity. SeY dietary addition elevated <i>Erysipelotrichia</i> (class), <i>Lachnospiraceae</i> (family), <i>Erysipelotrichaceae</i> (family) and <i>Ruminococcus_torques_group</i> (genus; <i>p</i> < .05). Analysis of microbial community-level phenotypes revealed that SeY supplementation decreased the microorganism abundance of facultatively anaerobic and potentially pathogenic phenotypes. Overall, SeY supplementation cannot significantly improve intestinal morphology; however, it modulated the composition of cecal microbiota toward a healthier gut.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2258188"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation the effect of dietary vitamin E, sesamin and thymoquinone bioactive compounds on immunological response, intestinal traits and MUC-2 gene expression in broiler Japanese quails (<i>Coturnix japonica</i>).","authors":"Yaser Rahimian, Farshid Kheiri, Mostafa Faghani","doi":"10.1080/10495398.2023.2259437","DOIUrl":"10.1080/10495398.2023.2259437","url":null,"abstract":"<p><p>The current study was performed to determine the effect of dietary vitamin E, sesamin and thymoquinone bioactive lignans derived from sesame and black seed on immunological response, intestinal traits and Mucin₂ gene expression in broiler quails. Three hundred and fifty (one days-old) quails were allotted to seven dietary treatments with five replicates as an experimental randomized design study. Treatments were basal diet as a control, control +100 and +200 mg of vitamin E, sesamin and thymoquinone per each kg of diet respectively. At 35 d of age, two quails from each pen were chosen, weighted, slaughtered, eviscerated and lymphoid organ relative weights were measured. Anti-body titers against Newcastle disease (ND), Sheep red blood cell (SRBC), and infectious bronchitis virus (IBV) and Avian influenza (AI) vaccination were determined. The serum activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum antioxidant activates such as superoxide dismutase (SOD),glutathione peroxidase(GPX), catalase (CAT) and total antioxidant capacity (TAC) were examined. The cell mediated immunity by dinitrochlorobenzene (DNCB) and phytohemagglutinin (PHA) challenges were assessed. The microflora populations of ileum, morphological traits of jejunum and mucin₂ gene expression were analyzed. Data showed that the lymphoid organ (thymus, spleen and Bursa) relative weights and antibody titer against HI, AI, SRBC and IB vaccination were increased compared to the control (<i>p</i> ≤ 0.05). Serum activities of ALP, ALT and AST were decreased under influences of dietary treatments (<i>p</i> ≤ 0.05). The serum antioxidant activates of GPX,SOD,CAT and TAC were increased and Increasing in mean skin thickness after DNCB challenge and decrease wing web swelling response to PHA mitojen injection were observed (<i>p</i> ≤ 0.05). <i>Salmonella enterica, E-coli</i> and <i>Coliforms</i> colonies were decrease and <i>Lactobacillus</i> colonies increased instead (<i>p</i> ≤ 0.05). The villus height and surface, crypt depth and goblet cells density were increased compared to the control (<i>p</i> ≤ 0.05). The expression of MUC₂ gene increased under influnces of vitamin E, sesamin and thymoquinone supplemented diets (<i>p</i> ≤ 0.05).</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2259437"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41095508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome resequencing-based analysis of plateau adaptation in Meiren yak (<i>Bos grunniens</i>).","authors":"Tong Wang, XiaoMing Ma, ChaoFan Ma, XiaoYun Wu, Ta ZhaXi, LiXin Yin, WeiGuo Li, YuFei Li, ChunNian Liang, Ping Yan","doi":"10.1080/10495398.2023.2298406","DOIUrl":"10.1080/10495398.2023.2298406","url":null,"abstract":"<p><p>The Meiren yak is an important genetic resource in Gansu Province, China. In this study, we aimed to explore the evolutionary history and population structure of the genetic resource of Meiren yak and to mine the characteristic genes of Meiren yak. We analysed a total of 93 yaks of eight yak breeds based on whole genome resequencing combined with population genomics and used θπ ratio and Fst method to screen the selected sites in the genome region. The results proved that Meiren yak can be used as a potential genetic resource in Gansu Province. The genes in Meiren yak with positive selection in selection signal analysis were subjected to the Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses, which indicated that the genes were related to the adaptability to high altitude and hypoxic environment. By analysing the genetic variation of Meiren yak at the genome-wide level, this study provided a theoretical basis for genetic improvement of Meiren yak and for the development of high-quality yak resources.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2298406"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139401518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}