Animal Biotechnology最新文献

{"title":"Composition and diversity of rumen mycobiota in Jiani yaks (<i>Bos grunniens</i> jiani): insights into microbial ecology and functions.","authors":"Yangji Cidan, Jia Wang, Hongzhuang Wang, Chang Xu, Yanbin Zhu, Muhammad Kasib Khan, Wangdui Basang, Kun Li","doi":"10.1080/10495398.2025.2476539","DOIUrl":"10.1080/10495398.2025.2476539","url":null,"abstract":"<p><p>This study aimed to explore the diversity and functions of rumen mycobiota in 14‑ (PLf) and 15‑rib (DLf) Jiani yaks using ITS sequencing. A total of 1,079,105 and 1,086,799 filtered sequences were obtained for the PLf and DLf groups, respectively, with 491 ASVs common to both. No significant difference regarding the α‑diversity of mycobiota within the two groups was observed. While β‑diversity analysis indicated that the abundance of fifteen (15) genera in the PLf group and two (2) genera in the DLf group was found to be significantly different (p &lt; 0.05). 16S rRNA sequencing results indicated that at the phylum level, in 14 ribs yaks Ascomycota, Basidiomycota, and Olpidiomycota, while in 15 rib yaks, Neocallimastigomycota, Mortierellomycota, and Rozellomycota were found to be significantly different (<i>p < 0.05</i>). At the genus level, <i>Rhodotorula</i>, <i>Kluyveromyces</i>, <i>Comoclathris</i>, <i>Arthrinium</i>, <i>Cladophialophora</i>, <i>Seimatosporium</i>, <i>Lambertella,</i> and <i>Sphacelotheca</i> in 14 rib yaks, and <i>Orpinomyces, Ustilago</i>, <i>Fusarium, Aspergillus, Caecomyces, Alternaria, Trichoderma</i> and <i>Acremonium</i> in 15 rib yaks were found to be significantly (<i>p < 0.05</i>) different. Predictive functional analysis based on ruminal fungal DNA sequences from 15‑rib yaks (DLf) demonstrated that genes involved in energy metabolism were upregulated. This study sheds novel insights into how genetic variations influence gut mycobiota in Jiani yak.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2476539"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cefoxitin inhibits the formation of biofilm involved in antimicrobial resistance MDR <i>Escherichia coli</i>.","authors":"Hailan Ma, Dacheng Liu, Chen Song, Hongliang Fan, Weiguang Zhou, Hongxia Zhao","doi":"10.1080/10495398.2025.2480176","DOIUrl":"10.1080/10495398.2025.2480176","url":null,"abstract":"<p><p>The study investigates the relationship between biofilm formation and antibiotic resistance in <i>Escherichia coli</i> (<i>E. coli</i>) isolated from calves. Using biochemical and molecular methods, we identified the isolates and assessed their biofilm-forming ability through an improved crystal violet staining method. The minimum inhibitory concentrations (MICs) of 18 antibiotics against the isolates were determined using the broth microdilution method. The impact of cefoxitin on biofilm formation was analyzed using laser scanning confocal microscopy (LSCM). Additionally, qRT-PCR was employed to evaluate the expression levels of biofilm-related genes (<i>luxS, motA, fliA, pfs, and csgD</i>) in response to varying cefoxitin concentrations. Results indicated a significant correlation between antimicrobial resistance (AMR) and biofilm formation ability. Cefoxitin effectively reduced biofilm formation of multidrug-resistant <i>E. coli</i> isolates at 1/2 and 1 MIC, with enhanced inhibition at higher concentrations. The QS-related genes <i>luxS, pfs, motA,</i> and <i>fliA</i> were downregulated, leading to decreased <i>csgD</i> expression. At 1/2 MIC, <i>csgD</i> expression was significantly reduced. In conclusion, cefoxitin inhibits biofilm formation in multidrug-resistant <i>E. coli</i> by down-regulating key genes, offering a potential strategy to mitigate resistance and control infections in calves caused by biofilm-positive <i>E. coli</i> isolates.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2480176"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perilipin1 mediates milk fat synthesis in bovine mammary epithelial cells through SREBP1 phosphorylation.","authors":"Benshun Yang, Meng Wang, Zhangqing Wu, Jianbing Tan, Yanhong Meng, Taoping Zhang, Linsen Zan, Wucai Yang","doi":"10.1080/10495398.2025.2497915","DOIUrl":"10.1080/10495398.2025.2497915","url":null,"abstract":"<p><p>This study investigates the role of Perilipin1 (PLIN1) in milk fat synthesis in bovine mammary epithelial cells (BMECs) and its regulatory mechanisms, aiming to provide a foundation for improving milk fat content through molecular breeding. BMECs were used as a model to analyze the effects of PLIN1 overexpression (OE-PLIN1) and interference (si-PLIN1) on milk fat synthesis and lipid-related gene expression using RT-qPCR, Western blot, and Oil Red O staining. Results show that OE-PLIN1 significantly enhances triglyceride (TAG) accumulation in BMECs (P < 0.01), upregulates lipid synthesis-related genes (such as PPARγ, C/EBPα, C/EBPβ, FABP4, FASN) (P < 0.05), and downregulates the mRNA expression of lipid breakdown-related genes (HSL, ATGL) (P < 0.05). Conversely, si-PLIN1 significantly reduces TAG accumulation (P < 0.05) and lowers the expression of lipid synthesis and breakdown genes (P < 0.05). Additionally, OE-PLIN1 combined with SREBP1 siRNA interference (si-SREBP1) did not have a significant impact on the mRNA and protein levels of SREBP1, but it significantly altered SREBP1's phosphorylation, indicating that SREBP1 interference inhibits PLIN1's effect on milk fat synthesis. This study suggests that PLIN1 promotes milk fat synthesis in BMECs via regulating SREBP1 activity, offering a new strategy for enhancing milk fat content in dairy cattle.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2497915"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis for cattle breeding improvement, progress and future perspectives in Peru: a review.","authors":"Deyanira Figueroa, Yolanda Romero, Lizeth A Heredia-Vilchez, Carlos Poemape, Wigoberto Alvarado, Carlos Quilcate","doi":"10.1080/10495398.2025.2547344","DOIUrl":"10.1080/10495398.2025.2547344","url":null,"abstract":"<p><p>Genomics offers a promising solution by enabling precise cattle selection and breeding to boost productivity and sustainability. In Peru, livestock plays a crucial role in the economy and food security. Despite its importance, the sector faces significant challenges, including poor pasture quality, limited conservation practices, a shortage of trained professionals, minimal use of genomic tools, and an incomplete understanding of the genetic potential of both native and introduced breeds. Since the 1940s, Peru has advanced in genetic improvement through artificial insemination, improved semen preservation, the establishment of a National Semen Bank, and the introduction of new breeds. Key developments have included embryo transfer, in vitro fertilization, and pioneering cloning efforts. Future perspectives for livestock genomics in Peru involve expanding bioinformatics capacity, improving genomic infrastructure, and integrating genomic selection into national breeding strategies. This review discusses the history, current status, challenges, and future perspectives of livestock genomics in Peru.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2547344"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome resequencing reveals new mutations in candidate genes for Beichuan-white goat prolificacya.","authors":"Aimin Zhou, Yi Ding, Xiaohui Zhang, Yugang Zhou, Yadong Liu, Tingjian Li, Long Xiao","doi":"10.1080/10495398.2023.2258166","DOIUrl":"10.1080/10495398.2023.2258166","url":null,"abstract":"<p><p>In this study, we evaluated the copy number variation in the genomes of two groups of Beichuan-white goat populations with large differences in litter size by <i>F</i>_ST method, and identified 1739 genes and 485 missense mutations in the genes subject to positive selection. Through functional enrichment, <i>ITGAV, LRP4, CDH23, TPRN, RYR2</i> and <i>CELSR1</i> genes, involved in <i>embryonic morphogenesis</i>, were essential for litter size trait, which received intensive attention. In addition, some mutation sites of these genes have been proposed (<i>ITGAV</i>: c.38C > T; <i>TPRN</i>: c.133A > T, c.1192A > G, c.1250A > C; <i>CELSR1</i>: c.7640T > C), whose allele frequencies were significantly changed in the high fecundity goat group. Besides, we found that new mutations at these sites altered the hydrophilicity and 3D structure of the protein. Candidate genes related to litter size in this study and their missense mutation sites were identified. These candidate genes are helpful to understand the genetic mechanism of fecundity in Beichuan white goat, and have important significance for future goat breeding.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2258166"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41105444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of alfalfa supplementary change dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) ratio on rumen fermentation and microbial function in Gansu alpine fine wool sheep (<i>Ovis aries</i>).","authors":"Qian Chen, Yun-Feng Cui, Zhao-Xi Zhang, Fu-Cheng Jiang, Xiang-Yu Meng, Jin-Jin Li, Da-Yong Cui, Jian-Lei Jia","doi":"10.1080/10495398.2023.2262539","DOIUrl":"10.1080/10495398.2023.2262539","url":null,"abstract":"<p><p>Bodyweight loss and rumen microbial dysfunction of grazing sheep was a challenge for the sheep production industry during cold season, which were considered to correlated with under-roughage-feeding. Alfalfa is a good roughage supplementary for ruminants, which can improve grazing sheep bodyweight-loss and rumen microbial dysfunction during grass-withering period. This study evaluated the effects of alfalfa hay supplementary change dietary non-fibrous carbohydrate/neutral detergent fiber (NFC/NDF) ratios on rumen fermentation and microbial function of Gansu alpine fine wool sheep during extreme cold season. 120 ewes (3-4 yrs) with an average body weight of 28.71 ± 1.22 kg were allocated randomly into three treatments, and fed NFC/NDF of 1.92 (H group), 1.11 (M group), and 0.68 (L group), respectively. This study was conducted for 107 d, including 7 d of adaption to the diets. The rumen fermentation parameters and microbial characteristics were measured after the end of feeding trials. The results showed that the concentrations of sheep body weight, nitrogen components (Total-N, Soluble protein-N and Ammonia-N), blood biochemical indices (LDH, BUN and CHO) and ruminal volatile fatty acids (TVFA and propionate) significantly increased with an increase in the proportion of NFC/NDF ratios (<i>p</i> < .05), and the acetate and acetate/propionat ratio presented a contrary decreasing trend (<i>p</i> < .05). A total of 1018 OTUs were obtained with 97% consistency. Ruminococcus, Ruminococcaceae and Prevotella were observed as the predominant phyla in ruminal fluid microbiota. Higher NFC/NDF ratios with Alfalfa supplementary increased the richness and diversity of ruminal fluid microbiota, and decreased ruminal fluid microbiota beta-diversity. Using clusters of orthologous groups (COG), the ruminal fluid microbiota of alfalfa supplementary feeding showed low immune pathway and high carbohydrate metabolism pathway. In summary, the study suggested that there was an increasing tendency in dietary NFC/NDF ratio of 1.92 in body weight, ruminal fermentation, microbial community composition and fermentation characteristics through developing alfalfa supplementary system.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2262539"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41100866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome resequencing revealed genomic variants and functional pathways related to adaptation in Indian yak populations.","authors":"Amod Kumar, Mahesh Dige, Saket Kumar Niranjan, Sonika Ahlawat, Reena Arora, Aneet Kour, Ramesh Kumar Vijh","doi":"10.1080/10495398.2023.2282723","DOIUrl":"10.1080/10495398.2023.2282723","url":null,"abstract":"<p><p>The present study aims to identify genomic variants through a whole genome sequencing (WGS) approach and uncover biological pathways associated with adaptation and fitness in Indian yak populations. A total of 30 samples (10 from each population) were included from Arunachali, Himachali and Ladakhi yak populations. WGS analysis revealed a total of 32171644, 27260825, and 32632460 SNPs and 4865254, 4429941, and 4847513 Indels in the Arunachali, Himachali, and Ladakhi yaks, respectively. Genes such as <i>RYR2</i>, <i>SYNE2</i>, <i>BOLA</i>, <i>HF1</i>, and the novel transcript <i>ENSBGRG00000011079</i> were found to have the maximum number of high impact variants in all three yak populations, and might play a major role in local adaptation. Functional enrichment analysis of genes harboring high impact SNPs revealed overrepresented pathways related to response to stress, immune system regulation, and high-altitude adaptation. This study provides comprehensive information about genomic variants and their annotation in Indian yak populations, thus would serve as a data resource for researchers working on the yaks. Furthermore, it could be well exploited for better yak conservation strategies by estimating population genetics parameters <i>viz</i>., effective population size, inbreeding, and observed and expected heterozygosity.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2282723"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138433038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to Imidacloprid under variable conditions disturbs the muscle fatty acid profile of a fresh water non target fish: <i>Labeo rohita</i>.","authors":"Shazia Qadir, Muhammad Latif, Wen-Feng Wu, Fengqin Feng, Wadi B Alonazi, Arwah Amjad, Chien-Chin Chen, Zia Ur Rehman, Adil Khan, Furhan Iqbal","doi":"10.1080/10495398.2024.2307020","DOIUrl":"10.1080/10495398.2024.2307020","url":null,"abstract":"<p><p>Economy of Pakistan is heavily dependent upon agriculture and extensive use of pesticide is quiet common to enhance the crop yield. Imidacloprid is among the first choice pesticides in Pakistan and it has been reported that through run off along with water it ends up in water bodies affecting non target aquatic fauna. Through the present investigation, we are reporting the effects of Imidacloprid on the fatty acids composition of a non-target, commercially important carp: <i>Labeo rohita</i>. Fish were exposed to sub lethal concentration of Imidacloprid (120 mgL¹) for 2, 4 and 8 days (short term) as well as for 16, 32 and 64 days (long term experimental conditions). Pesticide untreated controls were also maintained for each treatment. Following the specific Imidacloprid exposure, fatty acid composition (%) was determined in the muscle of all experimental groups by using gas chromatography. Fish exposed to Imidacloprid for 8 days had reduced Palmitic acid (<i>p</i> = 0.02) and elevated muscle Arachidic acid (<i>p</i> < 0.001) than control group. <i>Labeo rohita</i> exposed to the pesticide for 32 days had elevated muscle Oleic (<i>p</i> = 0.02) and Linoleic acid (<i>p</i> = 0.02) while fish exposed to Imidacloprid to 64 days had reduced muscle Palmitic (<i>p</i> = 0.04) and Oleic acid (<i>p</i> = 0.03). In conclusion, we are reporting that the exposure to sub lethal concentration of Imidacloprid disturb the muscle fatty acid composition of <i>Labeo rohita</i> that may affect its food quality. The effects were more pronounced under long term experimental conditions and were probably due to potentiating lipid peroxidation and disturbed fish metabolism upon Imidacloprid exposure.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2307020"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139519254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens.","authors":"Mustafa Bastamy, Ismail Raheel, Ahmed Elbestawy, Mohamed Diab, Enas Hammad, Lamiaa Elebeedy, Amal M El-Barbary, Ghadeer M Albadrani, Mohamed M Abdel-Daim, Mervat A Abdel-Latif, Ahmed Orabi","doi":"10.1080/10495398.2024.2309955","DOIUrl":"10.1080/10495398.2024.2309955","url":null,"abstract":"<p><p>Lysozymes, efficient alternative supplements to antibiotics, have several benefits in poultry production. In the present study, 120, one-day-old, Ross 308 broiler chickens of mixed sex, were allocated into 2 equal groups, lysozyme treated group (LTG) and lysozyme free group (LFG), to evaluate the efficacy of lysozyme (Lysonir^®) usage via both drinking water (thrice) and spray (once). LTG had better (<i>p</i> = 0.042) FCR, and higher European production efficiency factor compared to LFG (<i>p</i> = 0.042). The intestinal integrity score of LTG was decreased (<i>p</i> = 0.242) compared to that of LFG; 0.2 vs. 0.7. Higher (<i>p</i> ≤ 0.001) intestinal Lactobacillus counts were detected in chickens of LTG. Decreased (<i>p</i> ≤ 0.001) IL-1β and CXCL8 values were reported in LTG. The cellular immune modulation showed higher (<i>p</i> ≤ 0.001) opsonic activity (MΦ and phagocytic index) in LTG vs. LFG at 25 and 35 days. Also, higher (<i>p</i> ≤ 0.001) local, IgA, and humoral, HI titers, for both Newcastle, and avian influenza H5 viruses were found in LTG compared to LFG. In conclusion, microbial lysozyme could improve feed efficiency, intestinal integrity, <i>Lactobacillus</i> counts, anti-inflammatory, and immune responses in broiler chickens.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2309955"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139696820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock.","authors":"An Zongqi, Ana C Marshall, J M D R Jayawardana, Mike Weeks, Simon M Loveday, Warren McNabb, Nicolas Lopez-Villalobos","doi":"10.1080/10495398.2024.2379897","DOIUrl":"10.1080/10495398.2024.2379897","url":null,"abstract":"<p><p>The objectives of this study were to estimate genetic parameters for citric acid content (CA) and lactic acid content (LA) in sheep milk and to identify the associated candidate genes in a New Zealand dairy sheep flock. Records from 165 ewes were used. Heritability estimates based on pedigree records for CA and LA were 0.65 and 0.33, respectively. The genetic and phenotypic correlations between CA and LA were strong-moderate and negative. Estimates of genomic heritability for CA and LA were also high (0.85, 0.51) and the genomic correlation between CA and LA was strongly negative (-0.96 ± 0.11). No significant associations were found at the Bonferroni level. However, one intragenic SNP in C1QTNF1 (chromosome 11) was associated with CA, at the chromosomal significance threshold. Another SNP associated with CA was intergenic (chromosome 15). For LA, the most notable SNP was intragenic in CYTH1 (chromosome 11), the other two SNPs were intragenic in MGAT5B and TIMP2 (chromosome 11), and four SNPs were intergenic (chromosomes 1 and 24). The functions of candidate genes indicate that CA and LA could potentially be used as biomarkers for energy balance and clinical mastitis. Further research is recommended to validate the present results.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2379897"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}