Animal Biotechnology最新文献_第6页

Spatial distribution of maternal factors in pig mature oocytes. 猪成熟卵母细胞中母体因子的空间分布。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-08-26 DOI: 10.1080/10495398.2024.2394692

Fuquan Zhu, Meng Yang, Dayu Wang, Yuan Jiang, Chao Jia, Yanfeng Fu, Aochen Yu, Huijun Liu, Meixia Wang, Tingzhang Wang, Honglin Liu, Juan Li

{"title":"Spatial distribution of maternal factors in pig mature oocytes.","authors":"Fuquan Zhu, Meng Yang, Dayu Wang, Yuan Jiang, Chao Jia, Yanfeng Fu, Aochen Yu, Huijun Liu, Meixia Wang, Tingzhang Wang, Honglin Liu, Juan Li","doi":"10.1080/10495398.2024.2394692","DOIUrl":"https://doi.org/10.1080/10495398.2024.2394692","url":null,"abstract":"It is known that asymmetrical maternal transcripts play an important role in the cell fate of the early embryo, but few studies are available in mammal oocytes especially in pig. To investigate the spatial factors in pig oocytes, the oriented bisection was established for collecting karyoplasts (NSOs) and cytoplasts (SSOs) with more than 95% efficiency. Subsequently, RNA-Seq and LC-MS/MS analysis were performed on NSOs and SSOs. Although no differentially expressed genes (DEGs) could be detected between NSOs and SSOs, 89 of the differentially expressed proteins (DEPs) were detected, that 58 proteins higher expressed but 31 proteins lower expressed in NSOs compared with SSOs. These DEPs mainly participated in the 'cell cycle' and 'ribosome' pathway, while the up-regulated DEPs were mainly GO in 'spindle' and 'positive regulation of translation', and the down-regulated DEPs were in 'cytosolic small ribosomal subunit' and 'mRNA binding'. The up-regulated DEP SIRT5 which are related to the regulation of gene expression, epigenetic were further detected and revealed. A spatial asymmetry of maternal factors at the protein level was firstly detected in pig mature oocytes.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2394692"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of DHCR7 on adipocyte differentiation in goats. DHCR7 对山羊脂肪细胞分化的影响

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-29 DOI: 10.1080/10495398.2023.2298399

Zhibin Li, Tingting Hu, Ruiwen Li, Jinlan Li, Youli Wang, Yanyan Li, Yaqiu Lin, Yong Wang, Xing Jiani

{"title":"Effect of DHCR7 on adipocyte differentiation in goats.","authors":"Zhibin Li, Tingting Hu, Ruiwen Li, Jinlan Li, Youli Wang, Yanyan Li, Yaqiu Lin, Yong Wang, Xing Jiani","doi":"10.1080/10495398.2023.2298399","DOIUrl":"10.1080/10495398.2023.2298399","url":null,"abstract":"Cholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of DHCR7 in goat adipocytes remains unknown. Here, the effect of DHCR7 on the formation of subcutaneous and intramuscular fat in goats was investigated in vitro, and the result indicated that the mRNA level of DHCR7 showed a gradual downward trend in subcutaneous adipogenesis, but an opposite trend in intramuscular adipogenesis. In the process of subcutaneous preadipocytes differentiation, overexpression of DHCR7 inhibited the expression of adipocytes differentiation marker genes (CEBP/α, CEBP/β, SREBP1 and AP2), lipid metabolism-related genes (AGPAT6, FASN, SCD1 and LPL), and the lipid accumulation. However, in intramuscular preadipocyte differentiation, DHCR7 overexpression showed a promoting effect on adipocyte differentiation marker genes (CEBP/α, CEBP/β, PPARγ and SREBP1) and lipid metabolism-related genes (GPAM, AGPAT6, DGAT1 and SCD1) expression, and on lipid accumulation. In summary, our work demonstrated that DHCR7 played an important role in regulating adipogenic differentiation and lipid metabolism in preadipocytes in goats, which is of great significance for uncovering the underlying molecular mechanism of adipocyte differentiation and improving goat meat quality.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2298399"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exome-wide comparative analyses revealed differentiating genomic regions for performance traits in Indian native buffaloes. 全外显子组比较分析揭示了印度本地水牛性能性状的不同基因组区域。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-11-07 DOI: 10.1080/10495398.2023.2277376

Vishakha Uttam, Vikas Vohra, Supriya Chhotaray, Ameya Santhosh, Vikas Diwakar, Vaibhav Patel, Rajesh Kumar Gahlyan

{"title":"Exome-wide comparative analyses revealed differentiating genomic regions for performance traits in Indian native buffaloes.","authors":"Vishakha Uttam, Vikas Vohra, Supriya Chhotaray, Ameya Santhosh, Vikas Diwakar, Vaibhav Patel, Rajesh Kumar Gahlyan","doi":"10.1080/10495398.2023.2277376","DOIUrl":"10.1080/10495398.2023.2277376","url":null,"abstract":"In India, 20 breeds of buffalo have been identified and registered, yet limited studies have been conducted to explore the performance potential of these breeds, especially in the Indian native breeds. This study is a maiden attempt to delineate the important variants and unique genes through exome sequencing for milk yield, milk composition, fertility, and adaptation traits in Indian local breeds of buffalo. In the present study, whole exome sequencing was performed on Chhattisgarhi (n = 3), Chilika (n = 4), Gojri (n = 3), and Murrah (n = 4) buffalo breeds and after stringent quality control, 4333, 6829, 4130, and 4854 InDels were revealed, respectively. Exome-wide FST along 100-kb sliding windows detected 27, 98, 38, and 35 outlier windows in Chhattisgarhi, Chilika, Gojri, and Murrah, respectively. The comparative exome analysis of InDels and subsequent gene ontology revealed unique breed specific genes for milk yield (CAMSAP3), milk composition (CLCN1, NUDT3), fertility (PTGER3) and adaptation (KCNA3, TH) traits. Study provides insight into mechanism of how these breeds have evolved under natural selection, the impact of these events on their respective genomes, and their importance in maintaining purity of these breeds for the traits under study. Additionally, this result will underwrite to the genetic acquaintance of these breeds for breeding application, and in understanding of evolution of these Indian local breeds.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2277376"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71477252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel 22-bp InDel within FGF7 gene is significantly associated with growth traits in goat. FGF7基因中一个新的22bp的InDel与山羊的生长性状显著相关。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-10-23 DOI: 10.1080/10495398.2023.2262537

Xian-Feng Wu, Yuan Liu, Ying-Gang Wang, Fu Zhang, Wen-Yang Li

{"title":"A novel 22-bp InDel within FGF7 gene is significantly associated with growth traits in goat.","authors":"Xian-Feng Wu, Yuan Liu, Ying-Gang Wang, Fu Zhang, Wen-Yang Li","doi":"10.1080/10495398.2023.2262537","DOIUrl":"10.1080/10495398.2023.2262537","url":null,"abstract":"Fibroblast growth factor 7 (FGF7) is involved in lipid metabolism, which is considered as a candidate gene with close relation with muscle development by eGWAs and RNA-Seq analyses. To date, limited research has been conducted on the relationship between FGF7 gene and growth traits. The main objective of this work was to further investigate the association between novel InDel within FGF7 gene and growth traits in goat. Herein, FGF7 mRNA expression levels were investigated in various Fuqing goat tissues. We found that FGF7 gene was expressed in six adult goat tissues with the highest mRNA levels in adipose tissue. This result suggested that FGF7 gene might play a critical role in fat deposition. We also detected potential polymorphisms in Fuqing, Nubian and Jianyang Daer breeds. A 22-bp InDel polymorphism in FGF7 gene was detected in 396 goats and the three genotypes were designated as II, ID, and DD. Correlation analysis revealed that InDel polymorphism was significantly associated with growth traits (P < 0.05). Goats with genotypes ID and/or II had superior growth traits compared to those with genotype DD. In summary, our findings suggested that the 22-bp InDel within FGF7 gene could act as a molecular marker to improve the growth traits of goats in breeding programs.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2262537"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49688454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuron-derived neurotrophic factor promotes the differentiation of intramuscular and subcutaneous adipocytes in goat. 神经元衍生神经营养因子促进山羊肌肉和皮下脂肪细胞的分化

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-13 DOI: 10.1080/10495398.2024.2346223

An Li, Youli Wang, Ruiwen Li, Yaqiu Lin, Yanyan Li, Yong Wang, Wei Liu, Xiong Yan

{"title":"Neuron-derived neurotrophic factor promotes the differentiation of intramuscular and subcutaneous adipocytes in goat.","authors":"An Li, Youli Wang, Ruiwen Li, Yaqiu Lin, Yanyan Li, Yong Wang, Wei Liu, Xiong Yan","doi":"10.1080/10495398.2024.2346223","DOIUrl":"10.1080/10495398.2024.2346223","url":null,"abstract":"Adipocyte play an important role in human health and meat quality by influencing the tenderness, flavor, and juiciness of mutton It has been shown that neuron-derived neurotrophic factor (NENF) is closely related to energy metabolism and adipocyte differentiation in bovine. However, the role of NENF in the goats remains unclear. The aim of this study was to detect the expression of NENF in goat subcutaneous and intramuscular adipocytes, temporal expression profiles of the NENF, and overexpressed NENF on the differentiation of different adipocytes. In this study, PCR amplification successfully cloned the goat NENF gene with a fragment length of 521 bp. In addition, the time point of highest expression of NENF differed between these two adipocytes differentiation processes. Overexpression of NENF in intramuscular and subcutaneous adipocytes promoted the expression levels of differentiation markers CEBPβ and SREBP, which in turn promoted the differentiation of intramuscular and subcutaneous adipocytes. This study will provide basic data for further study of the role of goats in goat adipocyte differentiation and for the final elucidation of its molecular mechanisms in regulating goat adipocyte deposition.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2346223"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140915649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian granulosa cells. β-catenin和SF1之间的协同合作调节蛋鸡卵巢颗粒细胞中孕酮的合成。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-14 DOI: 10.1080/10495398.2024.2351975

Xueying Ma, Xu Han, Qin Zhang, Wenwen Wang, Hui Tang

{"title":"Synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian granulosa cells.","authors":"Xueying Ma, Xu Han, Qin Zhang, Wenwen Wang, Hui Tang","doi":"10.1080/10495398.2024.2351975","DOIUrl":"10.1080/10495398.2024.2351975","url":null,"abstract":"The development of ovarian follicles in poultry is a key factor affecting the performance of egg production. Ovarian follicle development is regulated via the Wnt/β-catenin signaling pathway, and β-catenin, encoded by CTNNB1, is a core component of this pathway. In this study, using ovary GCs from laying hens, we investigated the regulatory role of CTNNB1 in steroid synthesis. We found that CTNNB1 significantly regulates the expression of StAR and CYP11A1 (key genes related to progesterone synthesis) and the secretion of progesterone (P4). Furthermore, simultaneous overexpression of CTNNB1 and SF1 resulted in significantly higher levels of CYP11A1 and secretion of P4 than in cells overexpressing CTNNB1 or SF1 alone. We also found that in GCs overexpressing SF1, levels of CYP11A1 and secreted P4 were significantly greater than in controls. Silencing of CYP11A1 resulted in the inhibition of P4 secretion while overexpression of SF1 in CYP11A1-silenced cells restored P4 secretion to normal levels. Together, these results indicate that synergistic cooperation between the β-catenin and SF1 regulates progesterone synthesis in laying hen ovarian hierarchical granulosa cells to promote CYP11A1 expression.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2351975"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of biosurfactant-producing microbes isolated from the gastrointestinal system of broiler birds fed a commercial diet. 从商业日粮肉鸡胃肠系统中分离出的产生生物表面活性剂的微生物的分离和表征。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-10-10 DOI: 10.1080/10495398.2023.2263771

Ngavaite Chigede, Zedias Chikwambi, Irvin D T Mpofu, James Madzimure

{"title":"Isolation and characterization of biosurfactant-producing microbes isolated from the gastrointestinal system of broiler birds fed a commercial diet.","authors":"Ngavaite Chigede, Zedias Chikwambi, Irvin D T Mpofu, James Madzimure","doi":"10.1080/10495398.2023.2263771","DOIUrl":"10.1080/10495398.2023.2263771","url":null,"abstract":"Antimicrobial drug resistance (AMR) from improper use of antibiotics in various livestock products is a growing hazard for humans worldwide, with current death rate in excess of 700,000 per annum linked to the problem. Microorganisms are a rich source of structurally distinct bioactive compounds designed to protect the microbes and can offset AMR challenge. A study was conducted at Chinhoyi University of Technology to isolate, identify and characterize biosurfactant secreting microbes from broiler bird's gastrointestinal tract. Analysis of variance was performed in Genstat software. 16S rRNA technique was used to identify the DNA of isolates, annotated by similarity using BLASTn analysis against the NCBI nucleotide database. Phylogenetic analysis was performed on the BLASTn outcome to have an appreciation of the evolutionary genetic relationships. Small intestine-derived samples had a wider hemolytic activity of 5.6 mm, with a 39% emulsification index. At 98.29% sequence similarity, the bacterium producing biosurfactants was identified as an Escherichia coli strain similar to the 7.1994/NIST 0056 strain. The biosurfactant substance is a derivative of decane with beta lactams, tetracyclines and sulfa drugs properties which were responsible for the observed antibacterial activity. We recommend endogenous biosurfactant production optimization experiments and in-vivo trials to evaluate the potential impacts of a biosurfactant based feed additive in broilers.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2263771"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41181837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Aeromonas hydrophila infection on leptin receptor overlapping transcript expression in Rana amurensis. 嗜水气单胞菌感染对金眼蛙瘦素受体重叠转录本表达的影响

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-10-14 DOI: 10.1080/10495398.2024.2410742

Yugang Sun, Tong Wu, Zhaodong Chen, Huimin Ren, Yufen Liu, Peng Liu, Wenge Zhao

{"title":"Effect of Aeromonas hydrophila infection on leptin receptor overlapping transcript expression in Rana amurensis.","authors":"Yugang Sun, Tong Wu, Zhaodong Chen, Huimin Ren, Yufen Liu, Peng Liu, Wenge Zhao","doi":"10.1080/10495398.2024.2410742","DOIUrl":"https://doi.org/10.1080/10495398.2024.2410742","url":null,"abstract":"The leptin receptor overlapping transcript (LepROT) has been suggested to play several roles in immunomodulatory mechanisms; however, the understanding of its role in Rana amurensis immunity is still very limited. Here, we performed hematoxylin-eosin staining, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), immunofluorescence and western blotting to investigate the roles of LepROT in the immunomodulatory mechanism and the influence of its expression on the nuclear factor-kappa B (NF-κB) signaling pathway, such as the activation of IκB kinase and NF-кB, in amphibian resistance to infection with Aeromonas hydrophila (Ah). After Ah infection, the liver, lung, kidney, skin, muscle, and stomach of R. amurensis showed cell structure disturbance, bleeding, and texture abnormalities. In addition, the relative expression levels of LepROT, NF-кB, IKKα, and IKKβ were all upregulated after Ah infection; however, they showed time-dependent differential expression. The NF-кB signaling pathway exhibited robust expression levels, which might be explained by the positive feedback regulation function of LepROT. Overall, this study provides a basis for further assessment of the biological functions of LepROT and highlights its role in the regulation of immune mechanisms.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2410742"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype. 甲状腺转录组图谱揭示了与FecB BB基因型小尾寒羊多产有关的lncRNA和mRNA的不同调控。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-02-29 DOI: 10.1080/10495398.2024.2312393

Cheng Chang, Xiaoyun He, Ran Di, Xiangyu Wang, Miaoceng Han, Chen Liang, Mingxing Chu

{"title":"Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype.","authors":"Cheng Chang, Xiaoyun He, Ran Di, Xiangyu Wang, Miaoceng Han, Chen Liang, Mingxing Chu","doi":"10.1080/10495398.2024.2312393","DOIUrl":"10.1080/10495398.2024.2312393","url":null,"abstract":"Background: The thyroid gland is an important endocrine gland in animals that secretes thyroid hormones and acts on various organs throughout the body. lncRNAs are long non-coding RNAs that play an important role in animal reproduction; however, there is a lack of understanding of their expression patterns and potential roles in the thyroid gland of the Small Tail Han (STH) sheep. In this study, we used RNA-Seq technology to examine the transcriptome expression pattern of the thyroid from the luteal phase (LP) and follicular phase (FP) of FecB BB (MM) STH sheep.Results: We identified a total of 122 and 1287 differential expression lncRNAs (DELs) and differential expression mRNAs (DEGs), respectively, which were significantly differentially expressed. These DELs target genes and DEGs can be enriched in several signalling pathways related to the animal reproduction process.Conclusions: The expression profiles of DELs and DEGs in thyroid glands provide a more comprehensive resource for elucidating the reproductive regulatory mechanisms of STH sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2312393"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets. 仔猪饥饿应激诱导的小肠转录变化的 RNA-seq 分析。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-26 DOI: 10.1080/10495398.2023.2295931

Yijia Ma, Tong Guo, Jianqin Ding, Zhiling Dong, Yifei Ren, Chang Lu, Yan Zhao, Xiaohong Guo, Guoqing Cao, Bugao Li, Pengfei Gao

{"title":"RNA-seq analysis of small intestine transcriptional changes induced by starvation stress in piglets.","authors":"Yijia Ma, Tong Guo, Jianqin Ding, Zhiling Dong, Yifei Ren, Chang Lu, Yan Zhao, Xiaohong Guo, Guoqing Cao, Bugao Li, Pengfei Gao","doi":"10.1080/10495398.2023.2295931","DOIUrl":"10.1080/10495398.2023.2295931","url":null,"abstract":"Piglets may experience a variety of stress injuries, but the molecular regulatory mechanisms underlying these injuries are not well understood. In this study, we analysed the ileum of Large White (LW) and Mashen (MS) piglets at different times of starvation using chemical staining and transcriptome analysis. The intestinal barrier of piglets was damaged after starvation stress, but the intestinal antistress ability of MS piglets was stronger than LW piglets. A total of 8021 differentially expressed genes (DEGs) were identified in two breeds. Interestingly, the immune capacity (CHUK, TLR3) of MS piglets increased significantly after short-term starvation stress, while energy metabolism (NAGS, PLA2G12B, AGCG8) was predominant in LW piglets. After long-term starvation stress, the level of energy metabolism (PLIN5, PLA2G12B) was significantly increased in MS piglets. The expression of immune (HLA-DQB1, IGHG4, COL3A1, CD28, LAT) and disease (HSPA1B, MINPPI, ADH1C, GAL3ST1) related genes were significantly increased in two breeds of piglets. These results suggest that short-term stress mainly enhances immunity and energy metabolism in piglets, while long-term starvation produces greater stress on piglets, making it difficult for them to compensate for the damage to their bodies through self-regulation. This information can help improve the stress resistance of piglets through molecular breeding.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2295931"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0