Animal Biotechnology最新文献

{"title":"Single-step genome-wide association study of milk somatic cell scores across multi-cattle breeds in Ethiopia.","authors":"Tegegn Fantahun Chernet, Getinet Mekuriaw Tarekegn, Okeyo Mwai Ally, Selam Meseret, Raphael Mrode, Enyew Negussie, Zewdu Edea Bedada, Gebregziabher Gebreyohanes, Chinyere Ekine-Dzivenu, Asrat Tera, Tesfaye Sisay Tessema","doi":"10.1080/10495398.2025.2586262","DOIUrl":"10.1080/10495398.2025.2586262","url":null,"abstract":"<p><p>Mastitis, an inflammation of the bovine mammary gland, reduces dairy productivity and poses significant health risks to Ethiopian dairy cattle. This study aimed to identify genomic regions associated with milk somatic cell score (SCS) and estimate its genetic parameters. The dataset included 1647 phenotypic cows, 6964 genotyped animals, and 39,976 single nucleotide polymorphism (SNP) markers. A single-step genome-wide association study (ssGWAS) was conducted, accounting for fixed effects of parity, genomic breed composition, altitude, and lactation stage, and random effects of herd-year-calving-season and permanent environment. Genetic variance using 20 adjacent SNPs sliding windows explaining ≥1% of the total genetic variance were used for candidate genes and quantitative trait loci (QTL) identifications. The estimated heritability of SCS was 0.11 ± 0.06. Genomic regions on BTA 15, 19, and 26 were identified associated with SCS, encompassing 116 genes, including MPP7, MPP8, MMP13, BIRC2, BIRC3, BTRC, SRSF1, and MPO, which are involved in immunity, inflammation, apoptosis, antimicrobial defense, and tissue remodeling. Gene enrichment analysis revealed that the genes are involved collagen catabolic process and IL-17 signaling pathway. These findings provide insights into genomic regions that could be targeted in genomic selection to improve mastitis resistance in Ethiopian dairy cattle.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2586262"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12698049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of nitrogen isotopic fractionation as a biomarker for feed conversion efficiency in pigs using blood and hair samples.","authors":"Long Cheng, R J Smits, F R Dunshea, R J Dewhurst, J J Cottrell, S S Chauhan, J Luo, H Khanaki","doi":"10.1080/10495398.2025.2473494","DOIUrl":"10.1080/10495398.2025.2473494","url":null,"abstract":"<p><p>This study aimed to 1) Establish relationships between feed conversion efficiency (FCE; live weight gain/intake) and several biomarkers in pigs using blood and hair samples, and 2) Investigate the relative FCE performance of pigs from maternal vs. terminal genetic lines fed high vs. low energy diets. 80 male pigs (Large White x Landrace) were fed for 56 days. The terminal genetic line and pigs fed the high energy diet had 5% and 15% higher FCE than the maternal genetic line and pigs fed the low energy diet, respectively. Blood nitrogen isotopic fractionation (Δ¹⁵N; animal δ¹⁵N - feed δ¹⁵N) explained 34% more variation of FCE, compared with the blood insulin‑like growth factor‑1 (IGF‑1). The turnover rate of δ¹⁵N in plasma was faster than δ¹⁵N in blood, suggesting that blood and plasma δ¹⁵N can predict long‑term versus short‑term FCE changes. Pigs fed the high energy diets showed 13% higher live weight gain, 16% greater high standard carcass weight, and 38% higher carcass backfat than those on low‑energy diets. In conclusion, Δ¹⁵N is a more effective FCE biomarker for pigs compared to IGF‑1. Hair samples were less predictive of FCE than blood‑derived biomarkers, suggesting further refinement in the methodology of harvesting hair samples.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2473494"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology of bovine leukemia virus in cattle and phylogenetic analysis for determining its prevailing genotype in Khyber Pukhtunkhwa, Pakistan.","authors":"Farida Tahir, Umer Sadique, Farkhanda Tahir, Mikhlid H Almutairi, Abdulwahed Fahad Alrefaei, Shabana Naz, Rifat Ullah Khan, Naseer Khan Momand, Marco Ragni","doi":"10.1080/10495398.2025.2486029","DOIUrl":"10.1080/10495398.2025.2486029","url":null,"abstract":"<p><p>This research focused on assessing the molecular prevalence of Bovine Leukemia Virus (BLV) in different cattle farms throughout Khyber Pakhtunkhwa and characterizing the dominant BLV genotypes by analyzing partial sequences of the gp51 gene. A total of 1,250 blood samples were collected from cattle of both sexes, various age groups (<1 year, 1-3 years, 3-5 years, and >5 years), and different breeds (Friesian, Jersey, Sahiwal, Achai, and crossbred) from multiple cattle farms. Of the 1,250 samples tested, BLV was detected in 136 (10.88%) using nested PCR. Risk factor analysis revealed a significantly higher prevalence of BLV in exotic breeds and older cattle. To confirm the findings and genotype the BLV isolates, four PCR-positive samples were sequenced. Phylogenetic analysis identified the isolates as belonging to genotype I, closely related to GI BLV isolates from Japan. Furthermore, the isolates in this study formed a tightly clustered group, suggesting a common origin from an earlier virus introduced into the host population in the study area.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2486029"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality and microbial community analysis of solid-state fermented feed with mixed bacteria from corn silage.","authors":"Lanlan Zhang, Shiyu Yang, Zhihong Yan, Tianling Fu, Can Xiulan, Ping Li, Ceng Gong, Lili Cao","doi":"10.1080/10495398.2025.2507905","DOIUrl":"10.1080/10495398.2025.2507905","url":null,"abstract":"<p><p>The efficacy of microbial fermented feeds (FF) is highly dependent on microbial composition, underscoring the need for locally sourced, cost-effective formulations to advance sustainable livestock production. In this study, bacteria from corn silage (CSB) were screened for the preparation of solid-state fermented feed (SFF). In addition, for the purpose of comparison, purchased marketed fermentation bacteria (PMB) for SFF were also tested using the same methodology. Anaerobic fermentation was carried out by adding 5% CSB, PMB, and saline to conventional feeds at 37 °C, respectively. Then 10 g of fermentation samples was collected on days 0, 3, and 5, respectively. The composition and diversity of the microbial community were subsequently analyzed using high-throughput sequencing. The pH, titratable acidity (TA), and nutrient index of the feeds before and after fermentation were determined. High-throughput sequencing results showed that the dominant genera of CSB were <i>Weissella</i>, <i>Pseudomonas</i>, and <i>Bacteroidetes</i>, whereas the dominant genera of PMB were <i>Bacteroidetes</i>, <i>Bacillus</i>, <i>Pseudomonas</i>, and <i>Enterococcus</i>. The dominant genera in unfermented feeds (CK) were <i>Chlorophyta</i>, <i>Mitochondria</i>, and <i>Pseudomonas</i>. In addition, the dominant genera in SFF supplemented with CSB and PMB were <i>Weissella</i> and <i>Bacteroidetes</i>, respectively. Compared with CK, pH of SFF supplemented with CSB and PMB decreased gradually, and TA increased significantly and then decreased gradually during fermentation. Similarly, Crude fiber content was higher than CK in both CSB and PMB, while crude fat content was lower than CK for all of them. The study illustrates that CSB can be used to ferment porcine solid feeds and that CSB solid feed microorganisms may promote intestinal absorption of complex polysaccharides in pigs, which lays a certain theoretical foundation for the application of CSB to SFF.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2507905"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction statement.","authors":"","doi":"10.1080/10495398.2025.2528007","DOIUrl":"10.1080/10495398.2025.2528007","url":null,"abstract":"","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2528007"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary probiotic supplementation with <i>Lactococcus lactis</i> and <i>Bacillus velezensis</i> enhances growth performance, meat quality, blood profiles, and cecal and feed microbiota in growing rabbits.","authors":"Nada Elbaz, Elwy A Ashour, Samar S Bassiony, Ahmed I Elsherbeni, Samir A Mahgoub, Ali O Osman, Islam Sabike, Naif A Al-Gabri, Mahmoud Moustafa, Mohammed Al-Shehri, Mohamed E Abd El-Hack","doi":"10.1080/10495398.2025.2577947","DOIUrl":"10.1080/10495398.2025.2577947","url":null,"abstract":"<p><p>This study explored the effects of dietary supplementation with a probiotic blend of <i>Lactococcus lactis</i> subsp. <i>lactis</i> and <i>Bacillus velezensis</i> on the performance and health of growing rabbits. Ninety-six rabbits were allocated into three groups and fed for eight weeks on diets containing either no probiotic (control), a low-dose probiotic (1 ml/kg of each probiotic strain), or a high-dose probiotic (2 ml/kg of each probiotic strain). Probiotic inclusion, particularly at the higher level, significantly enhanced final body weight, feed conversion ratio, and overall performance. Improvements were also noted in carcass characteristics, with greater yields in both forequarters and hindquarters. Blood biochemical analysis revealed elevated antioxidant enzyme activity and immunoglobulin levels, alongside reductions in lipid fractions and better liver and kidney function indicators. Meat quality also benefited, as shown by lower pH, reduced water holding capacity and cooking loss, and more favorable color metrics. Microbial analysis of cecal and feed samples indicated a notable increase in beneficial bacteria and a decline in pathogenic species in the probiotic-treated groups. These findings suggest that combined probiotic supplementation is a promising strategy for enhancing growth performance, meat quality, and gut health in rabbit production systems to produce a good final meat product.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2577947"},"PeriodicalIF":2.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145420898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of supplementation with different dietary energy sources on semen quality in a cold-stressed boar model.","authors":"Jiayi Li, Yujie Wu, Min Yang, Zhengfeng Fang, Lianqiang Che, Shengyu Xu, Bin Feng, Yong Zhuo, Jian Li, Lun Hua, De Wu, Yan Lin","doi":"10.1080/10495398.2025.2536482","DOIUrl":"10.1080/10495398.2025.2536482","url":null,"abstract":"<p><p>Cold ambient temperatures decrease semen quality. However, the effects of dietary energy sources on semen quality under cold stress conditions remain unclear. In the present study, purebred Yorkshire boars (572 days old) were fed diets supplemented with either fat (FAT), amino acid complex (AA), or dietary fiber beet pulp (BP) during the winter. The experiment was conducted for 84 days. Results showed that BP supplementation increased semen volume, sperm count, plasma immunoglobulin M level and the relative abundance of fecal <i>norank_f_Erysipelotrichaceae</i> (positively correlated with sperm viability), while decreasing the levels of interleukin-1β and lipopolysaccharide-binding protein (<i>p</i> < 0.05). AA supplementation increased sperm density, progressive motility, and sperm velocity during weeks 7-12 (<i>p</i> < 0.05), while no significant effect was observed during weeks 1-6. The relative abundance of fecal <i>Lachnospiraceae_UCG-006</i> (positively correlated with sperm velocity) also was increased in the AA group. FAT supplementation had a minor effect on sperm progressive motility and average curvilinear velocity, which may be related to the increased relative abundance of fecal <i>T2WK15B57</i> (negatively correlated with semen quality). In summary, daily supplementation with BP and AA improves sperm quality under cold stress conditions, likely through improvements in inflammation, immunity and intestinal microflora.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2536482"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145720407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin alleviates metabolic-associated fatty liver disease by tuning hepatic lipid metabolism, oxidative stress and inflammation.","authors":"Ling Jiang, Rong Yi, Huan Chen, Shuwu Wu","doi":"10.1080/10495398.2024.2442351","DOIUrl":"10.1080/10495398.2024.2442351","url":null,"abstract":"<p><p>The natural flavonoid quercetin, which exhibits a range of biological activities, has been implicated in liver disease resistance in recent research. <i>In vivo</i> study attesting to quercetin's protective effect against metabolic-associated fatty liver disease (MAFLD) is inadequate, however. Here, our investigation explored the potential benefits of quercetin in preventing MAFLD in C57BL/6 mice fed a high-fat diet (HFD). The results revealed that quercetin ameliorated the aberrant enhancement of body and liver weight. The hepatic histological anomalie induced by MAFLD were also mitigated by quercetin. HFD-induced imbalance in serum LDL, HDL, AST, ALT, TG, and LDH was mitigated by quercetin. Mechanically, we found that quercetin improved lipid metabolism by reducing lipogenesis proteins including ACC, FASN, and SREBP-1c and enhancing β-oxidation proteins including PPARα and CPT1A. <i>In vitro</i> study demonstrated that quercetin regulated hepatic lipid metabolism by targeting SREBP-1c and PPARα. Additionally, quercetin enhanced the antioxidant capacity in HFD-treated mice by downregulating Nrf2 and HO-1 expressions and upregulating SOD and GPX1 expressions. The hyper-activation of inflammation was also restored by quercetin via eliminating the phosphorylation of IκBα and NF-κB p65. Collectively, our observations highlight that quercetin exerts hepatoprotective properties in MAFLD mice by regulating hepatic lipid metabolism, oxidative stress and inflammatory response.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2442351"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and microbiological effects of a microencapsulated probiotic consortium on the ruminal microbiota <i>in vivo and in vitro</i> systems.","authors":"Johanna Marcela Urán Velásquez, Mauricio Agudelo Rendón, Mariana Zapata, Wilyer García Arboleda, Sara Echeverri, Juan Camilo Arroyave Manco, Koen Venema, Juan Vasquez","doi":"10.1080/10495398.2025.2547345","DOIUrl":"10.1080/10495398.2025.2547345","url":null,"abstract":"<p><p>Fistulated animals and rumen simulation systems are essential for evaluating the effects of ingredients like probiotics, proposed as sustainable alternatives to growth-promoting antibiotics in ruminant nutrition. This study assessed the impact of microencapsulated probiotics on the structure and functionality of an initial ruminal microbial community using <i>in vivo</i> (IVV) and <i>in vitro</i> (IVT) systems. The IVT system was inoculated with rumen fluid obtained from the cattle animal used as IVV system. Over time, both systems were analysed for short-chain fatty acid (SCFA) production, microbial composition and functionality using next-generation sequencing. Physicochemical parameters were consistent across both systems and the inoculum, with an increase in propionate concentration observed. Although the microbial composition of IVV and IVT systems was highly similar (Pearson correlation of 0.869), significant differences in B-diversity were noted (<i>p</i> value = 0.023). The systems also exhibited high similarity in enzymatic profiles (correlation: 0.971) and metabolic pathways (correlation: 0.938), despite differences in functional B-diversity. Both systems showed increased production of fibrolytic enzymes, enhancing feed efficiency. The use of microencapsulated probiotics induced both taxonomic and functional changes in the initial microbial community of the IVT and IVV systems, which can be linked to the zootechnical effects of using probiotics as additives in ruminal animal nutrition.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2547345"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12674296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variation and mRNA expression of the <i>ELOVL6</i> and <i>CRTC2</i> genes in Kalmyk cattle.","authors":"Nurlybay Kazhgaliyev, Kaster Nurgulsim, Aizhan Makhanbetova, Dulat Ibrayev, Kymbat Shaikenova, Zhandos Hasen, Saltanat Amantay, Saniya Zhumagaziyeva, Ilmira Mukhametzharova, Akkair Bakytzhan, Elmira Adilbekova","doi":"10.1080/10495398.2025.2583795","DOIUrl":"10.1080/10495398.2025.2583795","url":null,"abstract":"<p><p>Kalmyk cattle are an important meat breed in Kazakhstan, valued for their strong physique, genetic stability, and adaptability. In this study, we investigated the mRNA expression of ELOVL fatty acid elongase 6 (<i>ELOVL6</i>) and CREB-regulated transcription coactivator 2 (<i>CRTC2</i>) across multiple tissues, and further examined their genetic variations and associations with growth and carcass traits in 200 Kalmyk cattle. Expression analysis showed that <i>CRTC2</i> was most highly expressed in the heart and liver, whereas <i>ELOVL6</i> was predominantly expressed in the spleen and large intestine, highlighting their tissue-specific expression patterns. In addition, we identified a polymorphic SNP (g.16511290A > G) in the 3'UTR of <i>ELOVL6</i>, with three genotypes (AA, AG, GG) and the G allele being dominant (0.520). Polymorphism information content (PIC) analysis indicated high genetic diversity at this locus. Importantly, this SNP was significantly associated with live weight and body oblique length (<i>p</i> < 0.05), and individuals carrying heterozygous AG genotype showed higher body weight and length. Collectively, these findings suggest that g.16511290A > G within <i>ELOVL6</i> may serve as useful molecular markers for body measurements and meat traits, providing valuable resources for marker-assisted selection in beef cattle breeding programs.</p>","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"36 1","pages":"2583795"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12875334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}