Animal Biotechnology最新文献_第5页

Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry and in-silico annotation. 利用高分辨率质谱法和内部注释绘制印度斑马（Sahiwal）精子的蛋白质组图谱。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-11-20 DOI: 10.1080/10495398.2024.2428402

Vanya Bhushan, Syed Azmal Ali, Abhishek Parashar, Sudarshan Kumar, Ashok Kumar Mohanty

{"title":"Mapping the proteome landscape of Indian Zebu (Sahiwal) spermatozoa using high-resolution mass spectrometry and in-silico annotation.","authors":"Vanya Bhushan, Syed Azmal Ali, Abhishek Parashar, Sudarshan Kumar, Ashok Kumar Mohanty","doi":"10.1080/10495398.2024.2428402","DOIUrl":"https://doi.org/10.1080/10495398.2024.2428402","url":null,"abstract":"Proteomic analysis of sperm cells offers significant insights into proteins' structural, functional, and localization aspects within biological systems. Sahiwal, a native Indian cattle breed, is well known for its disease resistance, calving ease, and resilience to drought. This study addressed the gap in Sahiwal's comprehensive sperm proteome profiling data. The research involved the global in-silico quantitative high-resolution mass spectrometry-based protein profiling of Indian Zebu sperm, identifying 4651 sperm proteins. Beyond mere identification, the study characterized these proteins at a sub-organellar level to facilitate a better understanding of their functional attributes. Gene Ontology analysis of sperm proteins facilitated the segregation of proteins based on their function, localization, and mode of action. The study revealed that despite the limited number of organelles, sperm cells encapsulate a wide array of crucial proteins, compensating for the deficiency of organelles through the presence of multifunctional proteins. Most identified sperm proteins actively participate in spermatogenesis, motility, acrosome reaction, capacitation, and seminal plasma binding, directly or indirectly. Notably, the results not only present the highest number of identified bovine sperm proteins but also hold the potential to pave the way for empirical research on sperm functionality, egg-sperm interaction, sperm-sex sorting biomarkers, sperm quality, and bull fertility.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2428402"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Core promoter identification and transcriptional regulation of porcine ACSL3 gene. 猪 ACSL3 基因的核心启动子识别和转录调控。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-11-25 DOI: 10.1080/10495398.2024.2430383

Xiaomin Li, Zijiao Guo, Xueying Ma, Huixin Liu, Wenwen Wang, Hui Tang

{"title":"Core promoter identification and transcriptional regulation of porcine ACSL3 gene.","authors":"Xiaomin Li, Zijiao Guo, Xueying Ma, Huixin Liu, Wenwen Wang, Hui Tang","doi":"10.1080/10495398.2024.2430383","DOIUrl":"https://doi.org/10.1080/10495398.2024.2430383","url":null,"abstract":"Intramuscular fat (IMF) content is an important factor that affects the edible and processing quality of pork. Studying the transcriptional regulation mechanisms of genes affecting intramuscular fat deposition can provide theoretical support for genetic improvement in pigs. Long-chain fatty acyl-CoA synthase 3 (ACSL3), as a key enzyme in the process of lipid synthesis in mammals. However, no information about the core promoter of the ACSL3 gene and its transcriptional regulation has been reported so far. In this experiment, we successfully cloned 3112 bp of the porcine ACSL3 gene promoter region. In order to find out the core promoter of the ACSL3 gene. The results indicated that the core promoter region of the ACSL3 gene is located from -111 bp to -59 bp upstream of the transcription initiation site (TSS). To identify the interaction between SP1 and the ACSL3 gene promoter, we mutated the predicted binding sites of ACSL3 gene promoter. The results showed that the activity of the promoter was decreased by site-specific mutagenesis of the SP1 transcription factor binding site, while overexpression of SP1 increased the expression of the ACSL3 gene. In summary, our study identified a core promoter region of the porcine ACSL3 gene, and the SP1 binding site is responsible for the promoter activity.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2430383"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of different mating ratios of broiler breeder on reproductive performance during post moult phase. 肉种鸡不同交配比例对换羽后阶段繁殖性能的影响

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI: 10.1080/10495398.2024.2398707

Mohammad A Elsagheer, Omar A Mohamed, Ibrahim M Khattab, Marian K Wadea

{"title":"Impact of different mating ratios of broiler breeder on reproductive performance during post moult phase.","authors":"Mohammad A Elsagheer, Omar A Mohamed, Ibrahim M Khattab, Marian K Wadea","doi":"10.1080/10495398.2024.2398707","DOIUrl":"10.1080/10495398.2024.2398707","url":null,"abstract":"Studies comparing mating ratio after forced molting are important for improving the efficiency of broiler breeder flocks. This study examined the effect of mating ratios on Egg production, chick weight, fertility, hatchability and embryonic mortality rate in Arbor Acres Plus broiler breeders post-moult phase. A total of 195 birds (62 weeks old), consisting of 15 cocks and 180 hens were randomly allocated with uniform body weight in a Completely Randomized Design into three groups based on the mating ratio (cock to hen). The groups included ratios of 1:10, 1:12, and 1:14, with each group replicated 5 times. Eggs were collected twice a day, and stored for 7 days at room temperature before placing them in the incubator. Results showed that mating ratios 1:10 and 1:12 had a positive effect on fertility rate and total hatchability compared to the 1:14 ratio. However, mating ratios did not affect laying rate, egg weight, egg mass, chick weight, hatchability of fertile eggs, and embryonic mortality rate. In conclusion, to reduce the cost of raising and caring cocks, a mating ratio of one cock to every twelve hens can be used for broiler breeders after forced molting to obtain the best fertility and hatching results.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2398707"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype. 甲状腺转录组图谱揭示了与FecB BB基因型小尾寒羊多产有关的lncRNA和mRNA的不同调控。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-02-29 DOI: 10.1080/10495398.2024.2312393

Cheng Chang, Xiaoyun He, Ran Di, Xiangyu Wang, Miaoceng Han, Chen Liang, Mingxing Chu

{"title":"Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype.","authors":"Cheng Chang, Xiaoyun He, Ran Di, Xiangyu Wang, Miaoceng Han, Chen Liang, Mingxing Chu","doi":"10.1080/10495398.2024.2312393","DOIUrl":"10.1080/10495398.2024.2312393","url":null,"abstract":"Background: The thyroid gland is an important endocrine gland in animals that secretes thyroid hormones and acts on various organs throughout the body. lncRNAs are long non-coding RNAs that play an important role in animal reproduction; however, there is a lack of understanding of their expression patterns and potential roles in the thyroid gland of the Small Tail Han (STH) sheep. In this study, we used RNA-Seq technology to examine the transcriptome expression pattern of the thyroid from the luteal phase (LP) and follicular phase (FP) of FecB BB (MM) STH sheep.Results: We identified a total of 122 and 1287 differential expression lncRNAs (DELs) and differential expression mRNAs (DEGs), respectively, which were significantly differentially expressed. These DELs target genes and DEGs can be enriched in several signalling pathways related to the animal reproduction process.Conclusions: The expression profiles of DELs and DEGs in thyroid glands provide a more comprehensive resource for elucidating the reproductive regulatory mechanisms of STH sheep.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2312393"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of chicken Relaxin3 gene: mRNA expression and response to reproductive hormone treatment in ovarian granulosa cells, and single nucleotide polymorphisms associated with egg laying traits in hens. 鸡 Relaxin3 基因的特征：卵巢颗粒细胞中 mRNA 的表达和对生殖激素处理的反应，以及与母鸡产蛋性状相关的单核苷酸多态性。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-06-28 DOI: 10.1080/10495398.2024.2370810

Chunfeng Zhang, Yi Sun, Li Kang, Yunliang Jiang

{"title":"Characterization of chicken Relaxin3 gene: mRNA expression and response to reproductive hormone treatment in ovarian granulosa cells, and single nucleotide polymorphisms associated with egg laying traits in hens.","authors":"Chunfeng Zhang, Yi Sun, Li Kang, Yunliang Jiang","doi":"10.1080/10495398.2024.2370810","DOIUrl":"10.1080/10495398.2024.2370810","url":null,"abstract":"As a protein structurally similar to insulin, relaxin3 (RLN3) plays a role in promoting arousal, suppressing depressive or anxious behaviors. Two studies revealed the increase of RLN3 expression during chicken follicle selection. In this study, by real-time quantitative PCR and luciferase assay, mRNA expression and single nucleotide polymorphisms (SNPs) of chicken RLN3 were investigated. The mRNA expression of chicken RLN3 was higher in the granulosa cell of hierarchal follicles (Post-GCs) than that of pre-hierarchal follicles (Pre-GCs). In Pre-GCs, the mRNA expression of chicken RLN3 was stimulated by FSH and progesterone; in Post-GCs, it was stimulated by higher concentration of estrogen and FSH, however, was inhibited by progesterone. Four SNPs including g.-655G > C, g-592G > A, g.-372T > A and g.-282G > C were identified in the critical promoter region from -1291 bp to -207 bp of chicken RLN3, among which g.-655G > C, and g-592G > A were associated with age at first laying and clutch size, respectively, in Zaozhuang Sunzhi chickens. At g.-655G > C and g-592G > A, allele C and allele A had higher transcriptional activity, respectively. These data suggest that RLN3 plays an important role in chicken follicle development and SNPs in its promoter region are potential DNA markers for improving egg production traits.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2370810"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of miR-361-5p promotes the induced activation of SHF-stem cells through FOXM1 mediated Wnt/β-catenin pathway in cashmere goats. 敲除 miR-361-5p 可通过 FOXM1 介导的 Wnt/β-catenin 通路促进羊绒山羊 SHF 干细胞的诱导活化。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-28 DOI: 10.1080/10495398.2024.2356110

Ruqing Xu, Man Bai, Yixing Fan, Yubo Zhu, Zeying Wang, Taiyu Hui, Qi Zhang, Xingwang Liu, Jialiang Zhang, Jincheng Shen, Wenlin Bai

{"title":"Knockdown of miR-361-5p promotes the induced activation of SHF-stem cells through FOXM1 mediated Wnt/β-catenin pathway in cashmere goats.","authors":"Ruqing Xu, Man Bai, Yixing Fan, Yubo Zhu, Zeying Wang, Taiyu Hui, Qi Zhang, Xingwang Liu, Jialiang Zhang, Jincheng Shen, Wenlin Bai","doi":"10.1080/10495398.2024.2356110","DOIUrl":"10.1080/10495398.2024.2356110","url":null,"abstract":"The inducing activation event of secondary hair follicle (SHF)-stem cells is considered a key biological process in the SHF regeneration, and the morphogenesis of cashmere fiber in cashmere goats. The miR-361-5p was essentially implicated in the induced activation of SHF-stem cells of cashmere goats, but its functional mechanisms are unclear. Here, we confirmed miR-361-5p was significantly downregulated in anagen SHF bugle of cashmere goats compared with that at telogen, and miR-361-5p expression was significantly lower in SHF-stem cells after activation than its counterpart before activation. Further, we found that miR-361-5p could negatively regulate the induced activation event of SHF-stem cells in cashmere goats. Mechanistically, through dual-luciferase reporter assays, miR-361-5p specifically bound with FOXM1 mRNA in SHF-stem cells of cashmere goats and negatively regulated the expression of FOXM1 gene. Also, through overexpression/knockdown analysis of FOXM1 gene, our results indicated that FOXM1 upregulated the expression of Wnt/β-catenin pathway related genes in SHF-stem cells. Moreover, based on TOP/FOP-flash Wnt report assays, the knockdown of miR-361-5p promotes the Wnt/β-catenin pathway activation through upregulating the FOXM1 expression in SHF-stem cells. Finally, we demonstrated that miR-361-5p negatively regulated the induced activation of SHF-stem cells through FOXM1 mediated Wnt/β-catenin pathway in cashmere goats.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2356110"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive cognition of yak (Bos grunniens) AIFM2 gene and its anti-ferroptosis role in bisphenol A-induced fetal fibroblast model. 牦牛（Bos grunniens）AIFM2基因的全面认知及其在双酚A诱导的胎儿成纤维细胞模型中的抗败血作用

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-07-22 DOI: 10.1080/10495398.2024.2377209

Yueyue Li, Hongmei Xu, Zifeng Ma, Qiao Li, Yan Xiong, Xianrong Xiong, Jian Li, Daoliang Lan, Wei Fu

{"title":"Comprehensive cognition of yak (Bos grunniens) AIFM2 gene and its anti-ferroptosis role in bisphenol A-induced fetal fibroblast model.","authors":"Yueyue Li, Hongmei Xu, Zifeng Ma, Qiao Li, Yan Xiong, Xianrong Xiong, Jian Li, Daoliang Lan, Wei Fu","doi":"10.1080/10495398.2024.2377209","DOIUrl":"https://doi.org/10.1080/10495398.2024.2377209","url":null,"abstract":"Apoptosis-inducing factor mitochondrion-associated 2 (AIFM2) has been identified as a gene with anti-ferroptosis properties. To explore whether AIFM2 exerts anti-ferroptosis role in yaks (Bos grunniens), we cloned yak AIFM2 gene and analyzed its biological characteristics. The coding region of AIFM2 had 1122 bp and encoded 373 amino acids, which was conserved in mammals. Next, RT-qPCR results showed an extensive expression of AIMF2 in yak tissues. Furthermore, we isolated yak skin fibroblasts (YSFs) and established a bisphenol A (BPA)-induced ferroptosis model to further investigate the role of AIFM2. BPA elevated oxidative stress (reactive oxygen species, ROS) and lipid peroxidation (malondialdehyde, MDA and BODIPY), and reduced cell viability and antioxidant capacity (glutathione, GSH), with the severity depending on the dosage. Of note, a supplement of Ferrostatin-1 (Fer), an inhibitor of ferroptosis, restored the previously mentioned indicators. Subsequently, we constructed an AIFM2 overexpression vector and designed AIFM2 specific interfering siRNAs, which were transfected into YSFs. The results showed that overexpressing AIFM2 alleviated ferroptosis, characterizing by significant changes of cell viability, ROS, BODIPY, MDA and GSH. Meanwhile, interfering AIFM2 aggravated ferroptosis, demonstrating the critical anti-ferroptosis role of the yak AIFM2 gene. This study shed light on further exploring the molecular mechanism of AIFM2 in plateau adaptability.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":"35 1","pages":"2377209"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide selection reveals candidate genes associated with multiple teats in Hu sheep. 全基因组选择揭示了与胡羊多乳头相关的候选基因。

IF 1.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-07-21 DOI: 10.1080/10495398.2024.2380766

Wen Zhou, Cheng-Long Zhang, Zhipeng Han, Xiaopeng Li, Xinyu Bai, Jieru Wang, Ruizhi Yang, Shudong Liu

引用次数: 0

Morpometric and molecular characterization of Surguli goat through CO1 gene in district Kohat. 通过 CO1 基因对科哈特地区的苏尔古力山羊进行形态计量和分子鉴定。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-12-23 DOI: 10.1080/10495398.2023.2290528

Muhammad Munir Khan, Syed Muhammad Suhail, Hafiz Abdul Majid, Ijaz Ahmad, Umer Sadique, Rajwali Khan, Iftikhar Ahmad, Asim Ijaz, Khalid Khan, Farhad Ali, Muhammad Saeed Khan, Ahmed A El-Mansi

{"title":"Morpometric and molecular characterization of Surguli goat through CO1 gene in district Kohat.","authors":"Muhammad Munir Khan, Syed Muhammad Suhail, Hafiz Abdul Majid, Ijaz Ahmad, Umer Sadique, Rajwali Khan, Iftikhar Ahmad, Asim Ijaz, Khalid Khan, Farhad Ali, Muhammad Saeed Khan, Ahmed A El-Mansi","doi":"10.1080/10495398.2023.2290528","DOIUrl":"10.1080/10495398.2023.2290528","url":null,"abstract":"The present study was designed with the aim to study morphometric characterization as well as phylogeny and diversity of the local Surguli goat at their breeding tract district Kohat through mitochondrial DNA region, i.e., Cytochrome C Oxidase Subunit One (CO1) gene. Morphometric data and blood samples were collected from thirty (30) pure goats. Morphometric analysis showed that sex had significant effect (p < 0.05) on body weight, body length, hearth girth and horn length while no significant effect (p > 0.05) was observed for other characteristics. The results also indicated that age had significant effect (p < 0.05) on height at rump, ear length, horn length and tail length while no significant effect (p > 0.05) was observed for other characteristics. The phylogenetic analysis through CO1 nucleotide sequences within nucleotide range 1-767 showed nine polymorphic sites segregating into eight haplotypes. The mean intraspecific diversity and mean interspecific diversity were calculated as 0.23 and 2.36%, respectively. Phylogenetic tree revealed that Capra Ibex and native Surguli goat have common ancestors. The morphometric and molecular results obtained from the present study can be exploited as a selection tool for breeding and overall improvement.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2290528"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens. 不同培养条件下拉曼光谱的测定：鱼类细菌病原体的初步研究。

IF 3.7 3区农林科学

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-03 DOI: 10.1080/10495398.2023.2299733

Ezgi Dinçtürk

{"title":"Determination of Raman spectrum under different culture conditions: preliminary research on bacterial fish pathogens.","authors":"Ezgi Dinçtürk","doi":"10.1080/10495398.2023.2299733","DOIUrl":"10.1080/10495398.2023.2299733","url":null,"abstract":"The intensive labour and time required for conventional methods to identify bacterial fish pathogens have revealed the need to develop alternative methods. Raman spectroscopy has been used in the rapid optical identification of bacterial pathogens in recent years as an alternative method in microbiology. Strains of bacterial fish pathogens (Vibrio anguillarum, Lactococcus garvieae and Yersinia ruckeri) that often cause infectious diseases in fish were here identified and analyzed in terms of their biochemical structures in different media and at different incubation times, and the data were specified by using Raman spectroscopy. The results demonstrated that Raman spectroscopy presents species-specific Raman spectra of each disease-causing bacteria and that it would be more appropriate to choose general microbiological media over selective media for routine studies. Additionally, it was found that species-specific band regions did not differ in 24- and 48-hour cultures, but there could be a difference in peak intensity which may lead to difficult characterization of spectrum. The current study, conducted for the first time with bacterial fish pathogens under different incubation conditions, is believed to provide a basis for the routine use of Raman spectroscopy for quick pathogen identification and the precise determination of the methodology for further research.","PeriodicalId":7836,"journal":{"name":"Animal Biotechnology","volume":" ","pages":"2299733"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139080440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0