Deciphering the miRNA transcriptome of granulosa cells from dominant and subordinate follicles at first follicular wave in goat.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2023-09-26 DOI:10.1080/10495398.2023.2259967

Jinzhu Meng, Yuanyuan Zhao, Xingchao Song, Qingming An, Zhenyang Wu

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Abstract

In goats, most follicles in the ovaries will be atresia and only a few dominant follicles (DFs) may eventually mature and ovulate at a follicular wave. To investigate the potential microRNAs (miRNAs) that regulate the expression of genes associated with follicular dominance or atresia, small RNA sequencing was performed on granulosa cells of DF and subordinate follicle at the first follicular wave in goats. A total of 108 differentially expressed miRNAs were detected in the two types of follicle granulosa cells: 16 upregulated miRNAs and 92 downregulated miRNAs. Kyoto Encyclopedia of Genes and Genomes analysis of the target genes showed that TKTL1, LOC102187810, LOC102184409 and ALDOA are closely associated with follicle dominance and are involved in the pentose phosphate pathway. Furthermore, a coexpression network of miRNAs and follicular dominance-related genes was constructed. The qPCR results well correlated with the small RNA sequencing data. Our findings provide new insight for exploring the molecular mechanism of miRNAs in regulating follicular development in goats.

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山羊第一次卵泡波时显性和次级卵泡颗粒细胞的miRNA转录组的破译。

在山羊中，卵巢中的大多数卵泡将是闭锁的，只有少数优势卵泡（DF）可能最终成熟并以卵泡波排卵。为了研究调节卵泡显性或闭锁相关基因表达的潜在微小RNA（miRNA），在山羊的第一个卵泡波对DF和次级卵泡的颗粒细胞进行了小RNA测序。在两种类型的卵泡颗粒细胞中共检测到108个差异表达的miRNA：16个上调的miRNA和92个下调的miRNA。京都基因和基因组百科全书对靶基因的分析表明，TKTL1、LOC102187810、LOC102184409和ALDOA与卵泡优势密切相关，并参与磷酸戊糖途径。此外，构建了miRNA和卵泡优势相关基因的共表达网络。qPCR结果与小RNA测序数据有很好的相关性。我们的发现为探索miRNA调节山羊卵泡发育的分子机制提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animal Biotechnology 工程技术-奶制品与动物科学

CiteScore

2.90

自引率

5.40%

发文量

230

审稿时长

>12 weeks

期刊介绍： Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes