Effect of a garlic drench on Galectin gene expression in ovine whole blood.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-13 DOI:10.1080/10495398.2024.2344208

SreeNavya Inupala, Md Rasel Uzzaman, Priyanka Pande, Sowmya Jagana, Mulumebet Worku

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Abstract

Garlic, known for its immune-modulating and antibiotic properties, contains lectins that possess antimicrobial and immunomodulatory effects. Galectins (Gals), which bind β-galactosides, play a role in modulating immunity and pathological processes. It is hypothesized that garlic's lectin components interfere with animal lectins. St. Croix sheep, known for their resistance to parasites and adaptability, are influenced by dietary supplements for innate immunity. This study evaluated the impact of garlic drench on Galectin gene expression in St. Croix sheep. Adult non-lactating ewes received either garlic juice concentrate or sterile distilled water for four weeks. Blood samples were collected, and plasma and whole blood cells were separated. Galectin secretion was assessed using a Sheep-specific ELISA, while Galectin gene transcription was analyzed through real-time PCR. Garlic administration upregulated LGALS-3 gene expression and significantly increased total plasma protein concentration. Garlic supplementation also affected Galectin secretion, with Gal-1, Gal-3, and Gal-9 showing differential effects.

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大蒜淋洗对绵羊全血中 Galectin 基因表达的影响

大蒜以其免疫调节和抗生素特性而闻名，它含有具有抗菌和免疫调节作用的凝集素。大蒜凝集素（Gals）与 β-半乳糖苷结合，在调节免疫和病理过程中发挥作用。据推测，大蒜的凝集素成分会干扰动物凝集素。圣克罗伊绵羊以抵抗寄生虫和适应性强而著称，其先天性免疫受到膳食补充剂的影响。本研究评估了大蒜淋洗对圣克罗伊绵羊凝集素基因表达的影响。成年非哺乳期母羊连续四周服用浓缩大蒜汁或无菌蒸馏水。采集血液样本，分离血浆和全血细胞。使用绵羊特异性酶联免疫吸附试验（ELISA）评估大蒜素分泌情况，并通过实时 PCR 分析大蒜素基因转录情况。大蒜能上调 LGALS-3 基因表达，并显著增加血浆总蛋白浓度。补充大蒜也会影响胰凝乳蛋白的分泌，其中Gal-1、Gal-3和Gal-9表现出不同的影响。

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来源期刊

Animal Biotechnology 工程技术-奶制品与动物科学

CiteScore

2.90

自引率

5.40%

发文量

230

审稿时长

>12 weeks

期刊介绍： Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes